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Journal articles on the topic 'Viral tracings'

Author: Grafiati

Published: 23 September 2022

Last updated: 19 July 2025

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1

El-Gohary, Yousef, John Wiersch, Sidhartha Tulachan, et al. "Intraislet Pancreatic Ducts Can Give Rise to Insulin-Positive Cells." Endocrinology 157, no. 1 (2016): 166–75. http://dx.doi.org/10.1210/en.2015-1175.

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Abstract A key question in diabetes research is whether new β-cells can be derived from endogenous, nonendocrine cells. The potential for pancreatic ductal cells to convert into β-cells is a highly debated issue. To date, it remains unclear what anatomical process would result in duct-derived cells coming to exist within preexisting islets. We used a whole-mount technique to directly visualize the pancreatic ductal network in young wild-type mice, young humans, and wild-type and transgenic mice after partial pancreatectomy. Pancreatic ductal networks, originating from the main ductal tree, wer

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Toma, Letitia, Adriana Mercan Stanciu, Anca Zgura, Nicolae Bacalbasa, Camelia Diaconu, and Laura Iliescu. "Electrocardiographic Changes in Liver Cirrhosis—Clues for Cirrhotic Cardiomyopathy." Medicina 56, no. 2 (2020): 68. http://dx.doi.org/10.3390/medicina56020068.

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Background and Objectives: Cirrhotic cardiomyopathy is a chronic cardiac dysfunction associated with liver cirrhosis, in patients without previous heart disease, irrespective of the etiology of cirrhosis. Electrocardiography (ECG) is an important way to evaluate patients with cirrhosis and may reveal significant changes associated with liver disease. Our study aimed to evaluate ECG changes in patients with diagnosed liver cirrhosis and compare them to patients with chronic hepatitis. Materials and Methods: We evaluated laboratory findings and ECG tracings in 63 patients with cirrhosis and 54 p

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3

Jansen, A., and A. Loewy. "Viral tracing of innervation." Science 265, no. 5168 (1994): 121–22. http://dx.doi.org/10.1126/science.8016646.

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4

Bevins, Sarah. "Tracing the Viral Network." BioScience 65, no. 11 (2015): 1100–1101. http://dx.doi.org/10.1093/biosci/biv113.

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5

Ugolini, Gabriella. "Advances in viral transneuronal tracing." Journal of Neuroscience Methods 194, no. 1 (2010): 2–20. http://dx.doi.org/10.1016/j.jneumeth.2009.12.001.

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Standish, Amelia, Lynn W. Enquist, and James S. Schwaber. "Response : Viral Tracing of Innervation." Science 265, no. 5168 (1994): 121–22. http://dx.doi.org/10.1126/science.265.5168.121.b.

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Standish, Amelia, Lynn W. Enquist, and James S. Schwaber. "Response : Viral Tracing of Innervation." Science 265, no. 5168 (1994): 121–22. http://dx.doi.org/10.1126/science.265.5168.121-b.

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8

Larsen, Philip Just. "Tracing autonomic innervation of the rat pineal gland using viral transneuronal tracing." Microscopy Research and Technique 46, no. 4-5 (1999): 296–304. http://dx.doi.org/10.1002/(sici)1097-0029(19990815/01)46:4/5<296::aid-jemt6>3.0.co;2-c.

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Lanciego, Jose L., and Floris G. Wouterlood. "Neuroanatomical tract-tracing techniques that did go viral." Brain Structure and Function 225, no. 4 (2020): 1193–224. http://dx.doi.org/10.1007/s00429-020-02041-6.

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10

Lynn W., Sun. "Viral and Non-viral Tracing of Cerebellar Corticonuclear and Vestibulorubral Projections in the Mouse." Open Journal of Neuroscinece 3, no. 1 (2013): 1. http://dx.doi.org/10.13055/ojns_3_1_3.130430.

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Ohara, Shinya, Ken-ichi Inoue, Masahiro Yamada, Ken-Ichiro Tsutsui, and Toshio Iijima. "Dual viral transneuronal tracing using recombinant rabies virus vectors." Neuroscience Research 58 (January 2007): S242. http://dx.doi.org/10.1016/j.neures.2007.06.596.

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Prasad, J. A., and Y. Chudasama. "Viral Tracing Identifies Parallel Disynaptic Pathways to the Hippocampus." Journal of Neuroscience 33, no. 19 (2013): 8494–503. http://dx.doi.org/10.1523/jneurosci.5072-12.2013.

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Sizemore, Rachel J., Sonja Seeger-Armbruster, Stephanie M. Hughes, and Louise C. Parr-Brownlie. "Viral vector-based tools advance knowledge of basal ganglia anatomy and physiology." Journal of Neurophysiology 115, no. 4 (2016): 2124–46. http://dx.doi.org/10.1152/jn.01131.2015.

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Viral vectors were originally developed to deliver genes into host cells for therapeutic potential. However, viral vector use in neuroscience research has increased because they enhance interpretation of the anatomy and physiology of brain circuits compared with conventional tract tracing or electrical stimulation techniques. Viral vectors enable neuronal or glial subpopulations to be labeled or stimulated, which can be spatially restricted to a single target nucleus or pathway. Here we review the use of viral vectors to examine the structure and function of motor and limbic basal ganglia (BG)

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14

Willhite, D. C., K. T. Nguyen, A. V. Masurkar, C. A. Greer, G. M. Shepherd, and W. R. Chen. "Viral tracing identifies distributed columnar organization in the olfactory bulb." Proceedings of the National Academy of Sciences 103, no. 33 (2006): 12592–97. http://dx.doi.org/10.1073/pnas.0602032103.

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15

Rinaman, L. "Anterograde Transneuronal Viral Tracing of Central Viscerosensory Pathways in Rats." Journal of Neuroscience 24, no. 11 (2004): 2782–86. http://dx.doi.org/10.1523/jneurosci.5329-03.2004.

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16

Braz, João M., Lynn W. Enquist, and Allan I. Basbaum. "Inputs to serotonergic neurons revealed by conditional viral transneuronal tracing." Journal of Comparative Neurology 514, no. 2 (2009): 145–60. http://dx.doi.org/10.1002/cne.22003.

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17

Mazzotti, Giorgia, Luca Bianco, Enrico Lavezzo, Martina Bado, Stefano Toppo, and Paolo Fontana. "Viral Network Analyzer (VirNA): A Novel Minimum Spanning Networks Algorithm for Investigating Viral Evolution." International Journal of Molecular Sciences 26, no. 5 (2025): 2008. https://doi.org/10.3390/ijms26052008.

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Next Generation Sequencing technologies are essential in public health surveillance for tracking pathogen evolution, spread, and the emergence of new variants. However, the extensive sequencing of viral genomes during recent pandemics has highlighted the limitations of traditional molecular phylogenetic algorithms in capturing fine-grained evolutionary details when analyzed sequences are highly similar and datasets are large-scale. VirNA (Viral Network Analyzer) addresses this challenge by reconstructing detailed mutation patterns and tracing pathogen evolutionary routes in specific geographic

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18

Liu, Junfang, Minhong Su, Xin Chen, Zhongli Li, Zekui Fang, and Li Yi. "Lipid-mediated biosynthetic labeling strategy for in vivo dynamic tracing of avian influenza virus infection." Journal of Biomaterials Applications 36, no. 9 (2022): 1689–99. http://dx.doi.org/10.1177/08853282211063298.

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Monitoring the infection behavior of avian influenza viruses is crucial for understanding viral pathogenesis and preventing its epidemics among people. A number of viral labeling methods have been utilized for tracking viral infection process, but most of them are laborious or decreasing viral activity. Herein we explored a lipid biosynthetic labeling strategy for dynamical tracking the infection of H5N1 pseudotype virus (H5N1p) in host. Biotinylated lipids (biotinyl Cap-PE) were successfully incorporated into viral envelope when it underwent budding process by taking advantage of host cell-de

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19

Loeb, Mark, Douglas MacPherson, Michele Barton, and Jan Olde. "Implementation of the Canadian Contingency Plan for a Case of Suspected Viral Hemorrhagic Fever." Infection Control & Hospital Epidemiology 24, no. 4 (2003): 280–83. http://dx.doi.org/10.1086/502202.

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AbstractObjective:To describe the implementation of the Canadian contingency plan for viral hemorrhagic fever (VHF) in response to a suspected case.Setting:A 300-bed, tertiary-care, university-affiliated hospital.Participants:A 32-year-old Congolese woman admitted to the hospital with suspected VHF in February 2001. Contact evaluation included hospital healthcare workers and laboratory staff.Intervention:Enhanced isolation precautions were implemented in the patient care setting to prevent nosocomial transmission. Contact tracing and evaluation of close and high-risk contacts with symptoms was

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20

Taylor, Richard, Callist Tindimugaya, John Barker, David Macdonald, and Robinah Kulabako. "Convergent Radial Tracing of Viral and Solute Transport in Gneiss Saprolite." Ground Water 48, no. 2 (2010): 284–94. http://dx.doi.org/10.1111/j.1745-6584.2008.00547.x.

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21

Wilmink, Gerald, Ilyssa Summer, David Marsyla, et al. "Real-Time Digital Contact Tracing: Development of a System to Control COVID-19 Outbreaks in Nursing Homes and Long-Term Care Facilities." JMIR Public Health and Surveillance 6, no. 3 (2020): e20828. http://dx.doi.org/10.2196/20828.

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Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can spread rapidly in nursing homes and long-term care (LTC) facilities. Symptoms-based screening and manual contact tracing have limitations that render them ineffective for containing the viral spread in LTC facilities. Symptoms-based screening alone cannot identify asymptomatic people who are infected, and the viral spread is too fast in confined living quarters to be contained by slow manual contact tracing processes. Objective We describe the development of a digital contact tracing system that LTC facilities can use

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22

Kuzmin, A. G., Y. A. Titiov, and A. Y. Zaitceva. "MASS SPECTROMETRIC DIAGNOSIS OF RECOVERY AFTER RESPIRATORY ILLNESS USING MACHINE LEARNING METHODS." BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES 1, no. 2022-20 (2022): 74–77. http://dx.doi.org/10.37747/2312-640x-2022-20-74-77.

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The express methodology of evaluation of mass-spectrometric parameters of gas composition of exhaled air for differential diagnostics of acute respiratory viral infections and tracing of dynamics of recovery after the disease was developed.

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23

Mullokandov, Gavriel, Gayathri Vijayakumar, Paul Leon, et al. "High-complexity extracellular barcoding using a viral hemagglutinin." Proceedings of the National Academy of Sciences 117, no. 6 (2020): 2767–69. http://dx.doi.org/10.1073/pnas.1919182117.

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While single-cell sequencing technologies have revealed tissue heterogeneity, resolving mixed cellular libraries into cellular clones is essential for many pooled screens and clonal lineage tracing. Fluorescent proteins are limited in number, while DNA barcodes can only be read after cell lysis. To overcome these limitations, we used influenza virus hemagglutinins to engineer a genetically encoded cell-surface protein barcoding system. Using antibodies paired to hemagglutinins carrying combinations of escape mutations, we developed an exponential protein barcoding system which can label 128 cl

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Clark, Iain C., Cristina Gutiérrez-Vázquez, Michael A. Wheeler, et al. "Barcoded viral tracing of single-cell interactions in central nervous system inflammation." Science 372, no. 6540 (2021): eabf1230. http://dx.doi.org/10.1126/science.abf1230.

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Cell-cell interactions control the physiology and pathology of the central nervous system (CNS). To study astrocyte cell interactions in vivo, we developed rabies barcode interaction detection followed by sequencing (RABID-seq), which combines barcoded viral tracing and single-cell RNA sequencing (scRNA-seq). Using RABID-seq, we identified axon guidance molecules as candidate mediators of microglia-astrocyte interactions that promote CNS pathology in experimental autoimmune encephalomyelitis (EAE) and, potentially, multiple sclerosis (MS). In vivo cell-specific genetic perturbation EAE studies

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25

Metts, Brent A., Galen D. Kaufman, and Adrian A. Perachio. "Polysynaptic inputs to vestibular efferent neurons as revealed by viral transneuronal tracing." Experimental Brain Research 172, no. 2 (2006): 261–74. http://dx.doi.org/10.1007/s00221-005-0328-z.

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26

Wickersham, Ian R., and Heather A. Sullivan. "Rabies Viral Vectors for Monosynaptic Tracing and Targeted Transgene Expression in Neurons." Cold Spring Harbor Protocols 2015, no. 4 (2015): pdb.prot072389. http://dx.doi.org/10.1101/pdb.prot072389.

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27

Rosani, Umberto, Maxwell Shapiro, Paola Venier, and Bassem Allam. "A Needle in A Haystack: Tracing Bivalve-Associated Viruses in High-Throughput Transcriptomic Data." Viruses 11, no. 3 (2019): 205. http://dx.doi.org/10.3390/v11030205.

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Bivalve mollusks thrive in environments rich in microorganisms, such as estuarine and coastal waters, and they tend to accumulate various particles, including viruses. However, the current knowledge on mollusk viruses is mainly centered on few pathogenic viruses, whereas a general view of bivalve-associated viromes is lacking. This study was designed to explore the viral abundance and diversity in bivalve mollusks using transcriptomic datasets. From analyzing RNA-seq data of 58 bivalve species, we have reconstructed 26 nearly complete and over 413 partial RNA virus genomes. Although 96.4% of t

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28

Banfield, Bruce W., Jessica D. Kaufman, Jessica A. Randall, and Gary E. Pickard. "Development of Pseudorabies Virus Strains Expressing Red Fluorescent Proteins: New Tools for Multisynaptic Labeling Applications." Journal of Virology 77, no. 18 (2003): 10106–12. http://dx.doi.org/10.1128/jvi.77.18.10106-10112.2003.

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ABSTRACT The transsynaptic retrograde transport of the pseudorabies virus Bartha (PRV-Bartha) strain has become an important neuroanatomical tract-tracing technique. Recently, dual viral transneuronal labeling has been introduced by employing recombinant strains of PRV-Bartha engineered to express different reporter proteins. Dual viral transsynaptic tracing has the potential of becoming an extremely powerful method for defining connections of single neurons to multiple neural circuits in the brain. However, the present use of recombinant strains of PRV expressing different reporters that are

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29

Lin, Xiaoxiao, Michelle Amalraj, Crisylle Blanton, et al. "Noncanonical projections to the hippocampal CA3 regulate spatial learning and memory by modulating the feedforward hippocampal trisynaptic pathway." PLOS Biology 19, no. 12 (2021): e3001127. http://dx.doi.org/10.1371/journal.pbio.3001127.

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The hippocampal formation (HF) is well documented as having a feedforward, unidirectional circuit organization termed the trisynaptic pathway. This circuit organization exists along the septotemporal axis of the HF, but the circuit connectivity across septal to temporal regions is less well described. The emergence of viral genetic mapping techniques enhances our ability to determine the detailed complexity of HF circuitry. In earlier work, we mapped a subiculum (SUB) back projection to CA1 prompted by the discovery of theta wave back propagation from the SUB to CA1 and CA3. We reason that thi

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30

Caini, Saverio, Chiara Martinoli, Carlo La Vecchia, et al. "SARS-CoV-2 Circulation in the School Setting: A Systematic Review and Meta-Analysis." International Journal of Environmental Research and Public Health 19, no. 9 (2022): 5384. http://dx.doi.org/10.3390/ijerph19095384.

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The contribution of children to viral spread in schools is still debated. We conducted a systematic review and meta-analysis of studies to investigate SARS-CoV-2 transmission in the school setting. Literature searches on 15 May 2021 yielded a total of 1088 publications, including screening, contact tracing, and seroprevalence studies. MOOSE guidelines were followed, and data were analyzed using random-effects models. From screening studies involving more than 120,000 subjects, we estimated 0.31% (95% confidence interval (CI) 0.05–0.81) SARS-CoV-2 point prevalence in schools. Contact tracing st

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31

Boccia, Angelo, Rossella Tufano, Veronica Ferrucci, et al. "SARS-CoV-2 Pandemic Tracing in Italy Highlights Lineages with Mutational Burden in Growing Subsets." International Journal of Molecular Sciences 23, no. 8 (2022): 4155. http://dx.doi.org/10.3390/ijms23084155.

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Tracing the appearance and evolution of virus variants is essential in the management of the COVID-19 pandemic. Here, we focus on SARS-CoV-2 spread in Italian patients by using viral sequences deposited in public databases and a tracing procedure which is used to monitor the evolution of the pandemic and detect the spreading, within the infected population of emergent sub-clades with a potential positive selection. Analyses of a collection of monthly samples focused on Italy highlighted the appearance and evolution of all the main viral sub-trees emerging at the end of the first year of the pa

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Schwarz, Lindsay A., Kazunari Miyamichi, Xiaojing J. Gao, et al. "Viral-genetic tracing of the input–output organization of a central noradrenaline circuit." Nature 524, no. 7563 (2015): 88–92. http://dx.doi.org/10.1038/nature14600.

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33

Sylvester, C. M., K. E. Krout, and A. D. Loewy. "Suprachiasmatic nucleus projection to the medial prefrontal cortex: a viral transneuronal tracing study." Neuroscience 114, no. 4 (2002): 1071–80. http://dx.doi.org/10.1016/s0306-4522(02)00361-5.

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Standish, A., L. Enquist, and J. Schwaber. "Innervation of the heart and its central medullary origin defined by viral tracing." Science 263, no. 5144 (1994): 232–34. http://dx.doi.org/10.1126/science.8284675.

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35

Song, C. Kay, Gary J. Schwartz, and Timothy J. Bartness. "Anterograde transneuronal viral tract tracing reveals central sensory circuits from white adipose tissue." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 296, no. 3 (2009): R501—R511. http://dx.doi.org/10.1152/ajpregu.90786.2008.

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The origins of the sympathetic nervous system (SNS) innervation of white adipose tissue (WAT) have been defined using the transneuronal viral retrograde tract tracer, pseudorabies virus. Activation of this SNS innervation is acknowledged as the principal initiator of WAT lipolysis. The central control of WAT lipolysis may require neural feedback to a brain-SNS-WAT circuit via WAT afferents. Indeed, conventional tract tracing studies have demonstrated that peripheral pseudounipolar dorsal root ganglion (DRG) sensory cells innervate WAT. The central nervous system projections of WAT afferents re

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36

Viney, Tim James, Kamill Balint, Daniel Hillier, et al. "Local Retinal Circuits of Melanopsin-Containing Ganglion Cells Identified by Transsynaptic Viral Tracing." Current Biology 17, no. 11 (2007): 981–88. http://dx.doi.org/10.1016/j.cub.2007.04.058.

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37

Anaclerio, Federico, Rossella Ferrante, Domitilla Mandatori, et al. "Different Strategies for the Identification of SARS-CoV-2 Variants in the Laboratory Practice." Genes 12, no. 9 (2021): 1428. http://dx.doi.org/10.3390/genes12091428.

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A considerable effort has been devoted in all countries to react to the COVID-19 pandemic by tracing infected individuals, containing the spread of the disease, identifying therapies, and producing and distributing vaccines. Currently, a significant concern is the appearance of variants of the virus that may frustrate these efforts by showing increased transmissibility, increased disease severity, reduced response to therapy or vaccines, and ability to escape diagnosis. All countries have therefore devoted a massive attempt to the identification and tracking of these variants, which requires a

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38

Etherington, Graham J., Susan M. Ring, Michael A. Charleston, Jo Dicks, Vic J. Rayward-Smith, and Ian N. Roberts. "Tracing the origin and co-phylogeny of the caliciviruses." Journal of General Virology 87, no. 5 (2006): 1229–35. http://dx.doi.org/10.1099/vir.0.81635-0.

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Caliciviruses infect a wide range of mammalian hosts and include the genus Norovirus, the major cause of food-borne viral gastroenteritis in humans. Using publicly available sequence data and phylogenetic analysis tools, the origins and virus–host co-phylogeny of these viruses were investigated. Here, evidence is presented in support of host switching by caliciviruses, but showing that zoonotic transfer does not appear to have occurred in the history of these viruses. The age or demography of the caliciviruses cannot yet be estimated with any firm degree of support, but further studies of this

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Mosa, Alexander I. "CRISPR-Based Diagnostics for Point-of-Care Viral Detection." International Journal of Translational Medicine 2, no. 2 (2022): 198–203. http://dx.doi.org/10.3390/ijtm2020017.

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Point-of-care detection of viral infection is required for effective contact-tracing, epidemiological surveillance, and linkage to care. Traditional diagnostic platforms relying on either antigen detection or nucleic amplification are limited by sensitivity and the need for costly laboratory infrastructure, respectively. Recently, CRISPR-based diagnostics have emerged as an alternative, combining equipment light workflows with high specificity and sensitivity. However, as a nascent technology, several outstanding challenges to widespread field deployment remain. These include the need for pre-

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40

Xiao, Ping-Jie, Chengwen Li, Aaron Neumann, and R. Jude Samulski. "Quantitative 3D Tracing of Gene-delivery Viral Vectors in Human Cells and Animal Tissues." Molecular Therapy 20, no. 2 (2012): 317–28. http://dx.doi.org/10.1038/mt.2011.250.

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Lappalainen, M. "Molecular epidemiology of viral pathogens and tracing of transmission routes: hepatitis-, calici- and hantaviruses." Journal of Clinical Virology 21, no. 3 (2001): 177–85. http://dx.doi.org/10.1016/s1386-6532(00)00162-1.

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Yamawaki, Takuma, Shinya Ohara, Ken-ichi Inoue, et al. "Dual viral tracing in the rat entorhinal-hippocampal circuit by recombinant rabies virus vectors." Neuroscience Research 65 (January 2009): S196. http://dx.doi.org/10.1016/j.neures.2009.09.1067.

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Cong, Wei, Yun Shi, Yanqing Qi, Jinyun Wu, Ling Gong, and Miao He. "Viral approaches to study the mammalian brain: Lineage tracing, circuit dissection and therapeutic applications." Journal of Neuroscience Methods 335 (April 2020): 108629. http://dx.doi.org/10.1016/j.jneumeth.2020.108629.

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Rouiller, Eric M., Mauricette Capt, Michel Dolivo, and Francois De Ribaupierre. "Tensor tympani reflex pathways studied with retrograde horseradish peroxidase and transneuronal viral tracing techniques." Neuroscience Letters 72, no. 3 (1986): 247–52. http://dx.doi.org/10.1016/0304-3940(86)90521-5.

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David, D., B. A. Yakobson, L. Gershkovich, and S. Gayer. "Tracing the regional source of rabies infection in an Israeli dog by viral analysis." Veterinary Record 155, no. 16 (2004): 496–97. http://dx.doi.org/10.1136/vr.155.16.496.

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Gerendai, I., I. E. Tóth, Z. Boldogkői, I. Medveczky, and B. Halász. "Central Nervous System Structures Labelled from the Testis Using the Transsynaptic Viral Tracing Technique." Journal of Neuroendocrinology 12, no. 11 (2001): 1087–95. http://dx.doi.org/10.1046/j.1365-2826.2000.00560.x.

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47

Gehrlach, Daniel A., Caroline Weiand, Thomas N. Gaitanos, et al. "A whole-brain connectivity map of mouse insular cortex." eLife 9 (September 17, 2020). http://dx.doi.org/10.7554/elife.55585.

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The insular cortex (IC) plays key roles in emotional and regulatory brain functions and is affected across psychiatric diseases. However, the brain-wide connections of the mouse IC have not been comprehensively mapped. Here, we traced the whole-brain inputs and outputs of the mouse IC across its rostro-caudal extent. We employed cell-type-specific monosynaptic rabies virus tracings to characterize afferent connections onto either excitatory or inhibitory IC neurons, and adeno-associated viral tracings to label excitatory efferent axons. While the connectivity between the IC and other cortical

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Cervantes, Orlando, Melissa R. Berg, Siddhartha G. Kapnadak, et al. "Testing pulmonary physiology in ventilated non‐human primates." Journal of Medical Primatology 53, no. 2 (2024). http://dx.doi.org/10.1111/jmp.12694.

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AbstractBackgroundAnimal models of respiratory viral infections are essential for investigating disease pathogenesis and the efficacy of antivirals and vaccine candidates. A major limitation in the research of respiratory diseases in animal models is correlating clinically relevant changes in pulmonary physiology with cellular and molecular mechanistic studies. Few animal models have captured and correlated physiologic changes in lung function and immune response within same experiment, which is critical given the heterogeneous nature of lung disease due to viral infections. In ventilated huma

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Liu, Qing, Yang Wu, Huadong Wang, Fan Jia, and Fuqiang Xu. "Viral Tools for Neural Circuit Tracing." Neuroscience Bulletin, September 22, 2022. http://dx.doi.org/10.1007/s12264-022-00949-z.

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50

Brown, Brandon L., Rachel M. Zalla, Courtney T. Shepard, et al. "Dual-Viral Transduction Utilizing Highly Efficient Retrograde Lentivirus Improves Labeling of Long Propriospinal Neurons." Frontiers in Neuroanatomy 15 (March 22, 2021). http://dx.doi.org/10.3389/fnana.2021.635921.

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The nervous system coordinates pathways and circuits to process sensory information and govern motor behaviors. Mapping these pathways is important to further understand the connectivity throughout the nervous system and is vital for developing treatments for neuronal diseases and disorders. We targeted long ascending propriospinal neurons (LAPNs) in the rat spinal cord utilizing Fluoro-Ruby (FR) [10kD rhodamine dextran amine (RDA)], and two dual-viral systems. Dual-viral tracing utilizing a retrograde adeno-associated virus (retroAAV), which confers robust labeling in the brain, resulted in a

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