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Journal articles on the topic 'Viral Vector Technology'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Metzner, Christoph, and Marianne Zaruba. "On the Relationship of Viral Particles and Extracellular Vesicles: Implications for Viral Vector Technology." Viruses 13, no. 7 (2021): 1238. http://dx.doi.org/10.3390/v13071238.

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Gene therapy vectors derived from different viral species have become a fixture in biomedicine, both for direct therapeutic intervention and as tools to facilitate cell-based therapies, such as chimeric antigen receptor-based immunotherapies. On the contrary, extracellular vesicles have only recently gained a massive increase in interest and, concomitantly, knowledge in the field has drastically risen. Viral infections and extracellular vesicle biology overlap in many ways, both with pro- and antiviral outcomes. In this review, we take a closer look at these interactions for the most prominent

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Stone, D., A. David, F. Bolognani, PR Lowenstein, and MG Castro. "Viral vectors for gene delivery and gene therapy within the endocrine system." Journal of Endocrinology 164, no. 2 (2000): 103–18. http://dx.doi.org/10.1677/joe.0.1640103.

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The transfer of genetic material into endocrine cells and tissues, both in vitro and in vivo, has been identified as critical for the study of endocrine mechanisms and the future treatment of endocrine disorders. Classical methods of gene transfer, such as transfection, are inefficient and limited mainly to delivery into actively proliferating cells in vitro. The development of viral vector gene delivery systems is beginning to circumvent these initial setbacks. Several kinds of viruses, including retrovirus, adenovirus, adeno-associated virus, and herpes simplex virus, have been manipulated f

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Sizemore, Rachel J., Sonja Seeger-Armbruster, Stephanie M. Hughes, and Louise C. Parr-Brownlie. "Viral vector-based tools advance knowledge of basal ganglia anatomy and physiology." Journal of Neurophysiology 115, no. 4 (2016): 2124–46. http://dx.doi.org/10.1152/jn.01131.2015.

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Viral vectors were originally developed to deliver genes into host cells for therapeutic potential. However, viral vector use in neuroscience research has increased because they enhance interpretation of the anatomy and physiology of brain circuits compared with conventional tract tracing or electrical stimulation techniques. Viral vectors enable neuronal or glial subpopulations to be labeled or stimulated, which can be spatially restricted to a single target nucleus or pathway. Here we review the use of viral vectors to examine the structure and function of motor and limbic basal ganglia (BG)

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Koshimizu, Yoshinori, Kaoru Isa, Kenta Kobayashi, and Tadashi Isa. "Double viral vector technology for selective manipulation of neural pathways with higher level of efficiency and safety." Gene Therapy 28, no. 6 (2021): 339–50. http://dx.doi.org/10.1038/s41434-020-00212-y.

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AbstractPathway-selective gene delivery would be critical for future gene therapy against neuropsychiatric disorders, traumatic neuronal injuries, or neurodegenerative diseases, because the impaired functions depend on neural circuits affected by the insults. Pathway-selective gene delivery can be achieved by double viral vector techniques, which combine an injection of a retrograde transport viral vector into the projection area of the target neurons and that of an anterograde viral vector into their somas. In this study, we tested the efficiency of gene delivery with different combinations o

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Hosseinkhani, Hossein, Abraham J. Domb, Ghorbanali Sharifzadeh, and Victoria Nahum. "Gene Therapy for Regenerative Medicine." Pharmaceutics 15, no. 3 (2023): 856. http://dx.doi.org/10.3390/pharmaceutics15030856.

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The development of biological methods over the past decade has stimulated great interest in the possibility to regenerate human tissues. Advances in stem cell research, gene therapy, and tissue engineering have accelerated the technology in tissue and organ regeneration. However, despite significant progress in this area, there are still several technical issues that must be addressed, especially in the clinical use of gene therapy. The aims of gene therapy include utilising cells to produce a suitable protein, silencing over-producing proteins, and genetically modifying and repairing cell fun

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Kreppel, Florian, and Claudia Hagedorn. "Episomes and Transposases—Utilities to Maintain Transgene Expression from Nonviral Vectors." Genes 13, no. 10 (2022): 1872. http://dx.doi.org/10.3390/genes13101872.

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The efficient delivery and stable transgene expression are critical for applications in gene therapy. While carefully selected and engineered viral vectors allowed for remarkable clinical successes, they still bear significant safety risks. Thus, nonviral vectors are a sound alternative and avoid genotoxicity and adverse immunological reactions. Nonviral vector systems have been extensively studied and refined during the last decades. Emerging knowledge of the epigenetic regulation of replication and spatial chromatin organisation, as well as new technologies, such as Crispr/Cas, were employed

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Zhu, Zihe. "Overview of CRISPR/Cas Gene Editing System and Its Carrier System." Highlights in Science, Engineering and Technology 73 (November 29, 2023): 406–12. http://dx.doi.org/10.54097/hset.v73i.14030.

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The CRISPR/Cas9 system, which is currently extensively employed in gene editing-related engineering, is a flexible immune system in bacteria and archaea that reacts to viruses, bacteriophages, and foreign DNA. Cas proteins can cut foreign DNA into small molecule fragments, which are then integrated into CRISPR arrays; When the relevant DNA invades again, it can be accurately identified and cut off. The discovery of the CRISPR system is a major breakthrough in biology because it operates more efficiently and concisely than the previous two generations of technology, providing a powerful tool fo

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8

Schnödt, Maria, Marco Schmeer, Barbara Kracher, et al. "DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations." Molecular Therapy - Nucleic Acids 5 (2016): e355. http://dx.doi.org/10.1038/mtna.2016.60.

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Saadi, Hogir. "Gene Therapy Approaches." Qubahan Academic Journal 1, no. 1 (2021): 52–56. http://dx.doi.org/10.48161/qaj.v1n1a35.

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Gene therapy can be described broadly as the transfer of genetic material to control a disease or at least to enhance a patient's clinical status. The transformation of viruses into genetic shuttles is one of the core principles of gene therapy, which will introduce the gene of interest into the target tissue and cells. To do this, safe strategies have been invented, using many viral and non-viral vector delivery. Two major methods have emerged: modification in vivo and modification ex vivo. For gene therapeutic approaches which are focused on lifelong expression of the therapeutic gene, retro

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Santos, Lana C., Antônio Márcio Santana Fernandes, Izabel Almeida Alves, et al. "Trends in Viral Vector-Based Vaccines for Tuberculosis: A Patent Review (2010–2023)." Vaccines 12, no. 8 (2024): 876. http://dx.doi.org/10.3390/vaccines12080876.

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Tuberculosis (TB) is an ancient global public health problem. Several strategies have been applied to develop new and more effective vaccines against TB, from attenuated or inactivated mycobacteria to recombinant subunit or genetic vaccines, including viral vectors. This review aimed to evaluate patents filed between 2010 and 2023 for TB vaccine candidates. It focuses on viral vector-based strategies. A search was carried out in Espacenet, using the descriptors “mycobacterium and tuberculosis” and the classification A61K39. Of the 411 patents preliminarily identified, the majority were related

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Cheng, Hao, Feng Zhang, and Yang Ding. "CRISPR/Cas9 Delivery System Engineering for Genome Editing in Therapeutic Applications." Pharmaceutics 13, no. 10 (2021): 1649. http://dx.doi.org/10.3390/pharmaceutics13101649.

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The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) systems have emerged as a robust and versatile genome editing platform for gene correction, transcriptional regulation, disease modeling, and nucleic acids imaging. However, the insufficient transfection and off-target risks have seriously hampered the potential biomedical applications of CRISPR/Cas9 technology. Herein, we review the recent progress towards CRISPR/Cas9 system delivery based on viral and non-viral vectors. We summarize the CRISPR/Cas9-inspired clinical trials and analyze th

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Pei, Duoji. "Comparative analysis of efficacy and safety of ChAdOx1 nCov-19 and AD26.COV2.S." Theoretical and Natural Science 21, no. 1 (2023): 195–202. http://dx.doi.org/10.54254/2753-8818/21/20230863.

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Vaccine technology has been going through some radical and incremental advancement over the years for its importance as the most crucial tool in the prevention and elimination of transmissive diseases. Since the outbreak of COVID-19, several vaccine platforms were being studied, developed, and entered the market. Among the platforms, vector viral vaccines attracted particular attention for the cooperation of viral vectors to deliver genetic material of the pathogens. While the mechanism has improved the flexibility of vaccine design and efficacy, the adverse events after vaccination were worth

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Sakuma, Toshie, Michael A. Barry, and Yasuhiro Ikeda. "Lentiviral vectors: basic to translational." Biochemical Journal 443, no. 3 (2012): 603–18. http://dx.doi.org/10.1042/bj20120146.

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More than two decades have passed since genetically modified HIV was used for gene delivery. Through continuous improvements these early marker gene-carrying HIVs have evolved into safer and more effective lentiviral vectors. Lentiviral vectors offer several attractive properties as gene-delivery vehicles, including: (i) sustained gene delivery through stable vector integration into host genome; (ii) the capability of infecting both dividing and non-dividing cells; (iii) broad tissue tropisms, including important gene- and cell-therapy-target cell types; (iv) no expression of viral proteins af

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Grimm, Dirk, Shangzhen Zhou, Hiroyuki Nakai, et al. "Preclinical in vivo evaluation of pseudotyped adeno-associated virus vectors for liver gene therapy." Blood 102, no. 7 (2003): 2412–19. http://dx.doi.org/10.1182/blood-2003-02-0495.

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Abstract We report the generation and use of pseudotyped adeno-associated viral (AAV) vectors for the liver-specific expression of human blood coagulation factor IX (hFIX). Therefore, an AAV-2 genome encoding the hfIX gene was cross-packaged into capsids of AAV types 1 to 6 using efficient, large-scale technology for particle production and purification. In immunocompetent mice, the resultant vector particles expressed high hFIX levels ranging from 36% (AAV-4) to more than 2000% of normal (AAV-1, -2, and -6), which would exceed curative levels in patients with hemophilia. Expression was dose-

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Seclì, Laura, Guido Leoni, Valentino Ruzza, et al. "Personalized Cancer Vaccines Go Viral: Viral Vectors in the Era of Personalized Immunotherapy of Cancer." International Journal of Molecular Sciences 24, no. 23 (2023): 16591. http://dx.doi.org/10.3390/ijms242316591.

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The aim of personalized cancer vaccines is to elicit potent and tumor-specific immune responses against neoantigens specific to each patient and to establish durable immunity, while minimizing the adverse events. Over recent years, there has been a renewed interest in personalized cancer vaccines, primarily due to the advancement of innovative technologies for the identification of neoantigens and novel vaccine delivery platforms. Here, we review the emerging field of personalized cancer vaccination, with a focus on the use of viral vectors as a vaccine platform. The recent advancements in vir

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Nouri, Shahideh, Nidá Salem, Jared C. Nigg, and Bryce W. Falk. "Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics." Journal of Virology 90, no. 5 (2015): 2434–45. http://dx.doi.org/10.1128/jvi.02793-15.

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ABSTRACTThe Asian citrus psyllid,Diaphorina citri, is the natural vector of the causal agent of Huanglongbing (HLB), or citrus greening disease. Together; HLB andD. citrirepresent a major threat to world citrus production. As there is no cure for HLB, insect vector management is considered one strategy to help control the disease, andD. citriviruses might be useful. In this study, we used a metagenomic approach to analyze viral sequences associated with the global population ofD. citri. By sequencing small RNAs and the transcriptome coupled with bioinformatics analysis, we showed that the viru

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Hebben, Matthias. "Collaboration, technology & innovation in the scale-up of viral vector manufacturing." Cell and Gene Therapy Insights 08, no. 08 (2022): 961–65. http://dx.doi.org/10.18609/cgti.2022.142.

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Apolonia, Luis. "The Old and the New: Prospects for Non-Integrating Lentiviral Vector Technology." Viruses 12, no. 10 (2020): 1103. http://dx.doi.org/10.3390/v12101103.

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Lentiviral vectors have been developed and used in multiple gene and cell therapy applications. One of their main advantages over other vectors is the ability to integrate the genetic material into the genome of the host. However, this can also be a disadvantage as it may lead to insertional mutagenesis. To address this, non-integrating lentiviral vectors (NILVs) were developed. To generate NILVs, it is possible to introduce mutations in the viral enzyme integrase and/or mutations on the viral DNA recognised by integrase (the attachment sites). NILVs are able to stably express transgenes from

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Fung, Hua, Andrew E. Sloan, Jane Reese, et al. "Viral Insertion Safety in Patients with Glioblastoma Who Received a Novel Lentiviral MGMT-P140K Gene Therapy to Protect Bone Marrow from Chemotherapy: No Dominant Clonal Evolution Observed with Chemo-Selection." Blood 124, no. 21 (2014): 4801. http://dx.doi.org/10.1182/blood.v124.21.4801.4801.

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Abstract INTRODUCTION: To protect normal bone marrow from chemotherapy in glioblastoma patients, we have developed a novel strategy by introducing a strong DNA repair protein, mutant (P140K) of human methylguanine methyltransferase (MGMT), into patients’ CD34+ hematopoietic progenitors (HPC) by lentiviral gene transfer leading to selective expansion of drug-resistant P140K-MGMT CD34+ cells and their myeloid and immune cell progeny. METHODS: To achieve long-term stable expression of the P140K-MGMT gene, we used a lentiviral vector which integrates into the host genome. However, viral insertion

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May, Mike. "Viral Vector Industry Boosts Product Quality and Quantity." Genetic Engineering & Biotechnology News 40, no. 3 (2020): 42–44. http://dx.doi.org/10.1089/gen.40.03.11.

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Macdonald, Gareth John. "Viral Vector Production Evolves to Meet Surging Demand." Genetic Engineering & Biotechnology News 43, no. 3 (2023): 38–40. http://dx.doi.org/10.1089/gen.43.03.12.

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Ferreira, Rafael G., Neal F. Gordon, Rick Stock, and Demetri Petrides. "Adenoviral Vector COVID-19 Vaccines: Process and Cost Analysis." Processes 9, no. 8 (2021): 1430. http://dx.doi.org/10.3390/pr9081430.

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The COVID-19 pandemic has motivated the rapid development of numerous vaccines that have proven effective against SARS-CoV-2. Several of these successful vaccines are based on the adenoviral vector platform. The mass manufacturing of these vaccines poses great challenges, especially in the context of a pandemic where extremely large quantities must be produced quickly at an affordable cost. In this work, two baseline processes for the production of a COVID-19 adenoviral vector vaccine, B1 and P1, were designed, simulated and economically evaluated with the aid of the software SuperPro Designer

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Fomina, E. G., E. E. Grigorieva, and A. S. Vladyko. "Recombinant Retroviral Particles: Technology of Poduction and Application as Positive Controls for PCR Diagnostics of Dangerous Viral Infections." Problems of Particularly Dangerous Infections, no. 2 (July 12, 2020): 115–21. http://dx.doi.org/10.21055/0370-1069-2020-2-115-121.

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Objective. Construction of positive control samples based on recombinant retroviral particles and their application in RT-PCR diagnostic assays for RNA detection of agents of dangerous and particularly dangerous viral infections.Materials and methods. Molecular biological, genetic engineering, and immunological methods were used: polymerase chain reaction, restriction, ligation, cloning, transformation, transfection, flow cytometry.Results and discussion. Technology of positive control samples producing based on recombinant virions has been developed and tested. It includes construction of ret

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Suvorov, A. N., Т. A. Kramskaya, T. V. Gupalova, Yu A. Desheva, and G. F. Leontieva. "Mucosal Vaccines against Bacterial and Viral Pathogens." Epidemiology and Vaccinal Prevention 22, no. 4 (2023): 4–11. http://dx.doi.org/10.31631/2073-3046-2023-22-4-4-11.

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The mucosal membranes of the human body play a crucial role in the development, maintenance, and regulation of barrier functions and immune homeostasis, representing an integral component of the overall immune system. Mucosal vaccines elicit immune processes in the lymphoid tissue associated with the mucosal membranes. A critical objective of mucosal immunization is the identification of an antigen delivery vector capable of ensuring optimal vaccine efficacy. The authors of this article have conducted extensive research on the probiotic properties of enterococci over an extended period. They e

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Yang Zhou, Jordi, Keittisak Suwan, and Amin Hajitou. "Initial Steps for the Development of a Phage-Mediated Gene Replacement Therapy Using CRISPR-Cas9 Technology." Journal of Clinical Medicine 9, no. 5 (2020): 1498. http://dx.doi.org/10.3390/jcm9051498.

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p53 gene (TP53) replacement therapy has shown promising results in cancer gene therapy. However, it has been hampered, mostly because of the gene delivery vector of choice. CRISPR-Cas9 technology (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) can knock out the mutated TP53 (mutTP53), but due to its large size, many viral vectors are not suitable or require implemented strategies that lower the therapeutic efficiency. Here, we introduced a bacteriophage or phage-based vector with the ability to target cancer cells and aimed to investigate the feasibility

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Davis, Jennifer, Margaret V. Westfall, Dewayne Townsend, et al. "Designing Heart Performance by Gene Transfer." Physiological Reviews 88, no. 4 (2008): 1567–651. http://dx.doi.org/10.1152/physrev.00039.2007.

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The birth of molecular cardiology can be traced to the development and implementation of high-fidelity genetic approaches for manipulating the heart. Recombinant viral vector-based technology offers a highly effective approach to genetically engineer cardiac muscle in vitro and in vivo. This review highlights discoveries made in cardiac muscle physiology through the use of targeted viral-mediated genetic modification. Here the history of cardiac gene transfer technology and the strengths and limitations of viral and nonviral vectors for gene delivery are reviewed. A comprehensive account is gi

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Kaynarcalidan, Onur, Sara Moreno Mascaraque, and Ingo Drexler. "Vaccinia Virus: From Crude Smallpox Vaccines to Elaborate Viral Vector Vaccine Design." Biomedicines 9, no. 12 (2021): 1780. http://dx.doi.org/10.3390/biomedicines9121780.

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Various vaccinia virus (VACV) strains were applied during the smallpox vaccination campaign to eradicate the variola virus worldwide. After the eradication of smallpox, VACV gained popularity as a viral vector thanks to increasing innovations in genetic engineering and vaccine technology. Some VACV strains have been extensively used to develop vaccine candidates against various diseases. Modified vaccinia virus Ankara (MVA) is a VACV vaccine strain that offers several advantages for the development of recombinant vaccine candidates. In addition to various host-restriction genes, MVA lacks seve

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May, Mike. "Gene Therapy Dollar Is Waiting on Viral Vector Dime." Genetic Engineering & Biotechnology News 40, no. 2 (2020): 36–38. http://dx.doi.org/10.1089/gen.40.02.12.

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Tatineni, Satyanarayana, Lucy R. Stewart, Hélène Sanfaçon, Xiaofeng Wang, Jesús Navas-Castillo, and M. Reza Hajimorad. "Fundamental Aspects of Plant Viruses−An Overview on Focus Issue Articles." Phytopathology® 110, no. 1 (2020): 6–9. http://dx.doi.org/10.1094/phyto-10-19-0404-fi.

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Given the importance of and rapid research progress in plant virology in recent years, this Focus Issue broadly emphasizes advances in fundamental aspects of virus infection cycles and epidemiology. This Focus Issue comprises three review articles and 18 research articles. The research articles cover broad research areas on the identification of novel viruses, the development of detection methods, reverse genetics systems and functional genomics for plant viruses, vector and seed transmission studies, viral population studies, virus–virus interactions and their effect on vector transmission, a

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Wei, Mingshan, and Yitong Wu. "Application of CRISPR-Cas9 technology in the treatment of Triple Negative Breast Cancer." Theoretical and Natural Science 75, no. 1 (2025): 115–25. https://doi.org/10.54254/2753-8818/2024.la19360.

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Since its discovery in bacterial cells, the CRISPR-Cas9 system has gained popularity as a practical approach to modifying the genomes. The ability of CRISPR-Cas9 to perform precise gene editing made it a potent therapeutic option for cancer treatment. This article will provide a basic overview of the working mechanism of CRISPR-Cas9, the different variants of CRISPR systems, and the present application of CRISPR-Cas9 in cancer treatment. The role of CRISPR-Cas9 mediated gene therapy for triple-negative breast cancer (TNBC) one of the most aggressive types of cancer is investigated by focusing

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Mahmood, Muhammad Arslan, Rubab Zahra Naqvi, Saleem Ur Rahman, Imran Amin, and Shahid Mansoor. "Plant Virus-Derived Vectors for Plant Genome Engineering." Viruses 15, no. 2 (2023): 531. http://dx.doi.org/10.3390/v15020531.

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Advances in genome engineering (GE) tools based on sequence-specific programmable nucleases have revolutionized precise genome editing in plants. However, only the traditional approaches are used to deliver these GE reagents, which mostly rely on Agrobacterium-mediated transformation or particle bombardment. These techniques have been successfully used for the past decades for the genetic engineering of plants with some limitations relating to lengthy time-taking protocols and transgenes integration-related regulatory concerns. Nevertheless, in the era of climate change, we require certain fas

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Fernandez-Garcia, Laura, Olga Pacios, Mónica González-Bardanca, et al. "Viral Related Tools against SARS-CoV-2." Viruses 12, no. 10 (2020): 1172. http://dx.doi.org/10.3390/v12101172.

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At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2. As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to fight against it. Here we summarize the recent research on viruses employed as therapy and diagnostic of COVID-19: (i) viral-vector vaccines both in clinical trials and pre-clinical phases; (ii) the use of bacteriophages to find antibodies specific to this virus and some studies of how to use the bacteriophages themselves as a treatment against

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Su, Cheng-Huang, Yih-Jer Wu, Hsueh-Hsiao Wang, and Hung-I. Yeh. "Nonviral gene therapy targeting cardiovascular system." American Journal of Physiology-Heart and Circulatory Physiology 303, no. 6 (2012): H629—H638. http://dx.doi.org/10.1152/ajpheart.00126.2012.

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The goal of gene therapy is either to introduce a therapeutic gene into or replace a defective gene in an individual's cells and tissues. Gene therapy has been urged as a potential method to induce therapeutic angiogenesis in ischemic myocardium and peripheral tissues after extensive investigation in recent preclinical and clinical studies. A successful gene therapy mainly relies on the development of the gene delivery vector. Developments in viral and nonviral vector technology including cell-based gene transfer will further improve transgene delivery and expression efficiency. Nonviral appro

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Wang, Haoran, Jiaxin Tian, Jing Zhao, Ye Zhao, Huiming Yang, and Guozhong Zhang. "Current Status of Poultry Recombinant Virus Vector Vaccine Development." Vaccines 12, no. 6 (2024): 630. http://dx.doi.org/10.3390/vaccines12060630.

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Inactivated and live attenuated vaccines are the mainstays of preventing viral poultry diseases. However, the development of recombinant DNA technology in recent years has enabled the generation of recombinant virus vector vaccines, which have the advantages of preventing multiple diseases simultaneously and simplifying the vaccination schedule. More importantly, some can induce a protective immune response in the presence of maternal antibodies and offer long-term immune protection. These advantages compensate for the shortcomings of traditional vaccines. This review describes the constructio

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Belousova, Natalya, Galina Mikheeva, Juri Gelovani, and Victor Krasnykh. "Modification of Adenovirus Capsid with a Designed Protein Ligand Yields a Gene Vector Targeted to a Major Molecular Marker of Cancer." Journal of Virology 82, no. 2 (2007): 630–37. http://dx.doi.org/10.1128/jvi.01896-07.

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ABSTRACT The future of genetic interventions in humans critically depends on the selectivity and efficiency of gene transfer to target tissues. The viral gene vectors explored to date cannot selectively transduce the desired targets. While substantial progress has been made in developing targeting strategies for adenovirus (Ad) vectors, future advances in this direction are severely limited by the shortage of naturally existing molecules available for use as targeting ligands. This shortage is due to fundamental and irresolvable differences at the level of both posttranslational modifications

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Karhinen, Minna, Elisa M. Nurminen, Piia Valonen, et al. "706. Consistent Viral Vector Manufacturing for Phase III Using iCELLis 500 Fixed-Bed Technology." Molecular Therapy 24 (May 2016): S279. http://dx.doi.org/10.1016/s1525-0016(16)33514-6.

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Karpe, Shubham, Khushi Gupta, Ganesh Vyas, Princy Rana, Fahad Khan, and Rajesh Kumar. "Virosome: A vector in vaccine delivery." BIO Web of Conferences 86 (2024): 01020. http://dx.doi.org/10.1051/bioconf/20248601020.

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These days vaccines are considered the best economical and effective technique for preventing and handling bacterial infections, like human papillomavirus (HPV) or meningitis, allergies, autoimmune Relevant Disorders, Microbial Infections, and many more viral diseases. Annually millions of lives are saved from death-causing infections and other relevant disorders with the help of proper immunization with the help of vaccines. But still, many diseases are not yet preventable by vaccines and there is a chance of the improvement of current vaccines with help of many approaches. One of these is a

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Khakhar, Arjun, Cecily Wang, Ryan Swanson, et al. "VipariNama: RNA viral vectors to rapidly elucidate the relationship between gene expression and phenotype." Plant Physiology 186, no. 4 (2021): 2222–38. http://dx.doi.org/10.1093/plphys/kiab197.

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Abstract Synthetic transcription factors have great promise as tools to help elucidate relationships between gene expression and phenotype by allowing tunable alterations of gene expression without genomic alterations of the loci being studied. However, the years-long timescales, high cost, and technical skill associated with plant transformation have limited their use. In this work, we developed a technology called VipariNama (ViN) in which vectors based on the tobacco rattle virus are used to rapidly deploy Cas9-based synthetic transcription factors and reprogram gene expression in planta. W

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Labant, MaryAnn. "The Next Generation of Cell Factories for Viral Vector Production." Genetic Engineering & Biotechnology News 41, S2 (2021): S10—S13. http://dx.doi.org/10.1089/gen.41.s2.04.

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Lisziewicz, Julianna, Dmitry I. Gabrilovich, Georg Varga, et al. "Induction of Potent Human Immunodeficiency Virus Type 1-Specific T-Cell-Restricted Immunity by Genetically Modified Dendritic Cells." Journal of Virology 75, no. 16 (2001): 7621–28. http://dx.doi.org/10.1128/jvi.75.16.7621-7628.2001.

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ABSTRACT A novel technology combining replication- and integration-defective human immunodeficiency virus type 1 (HIV-1) vectors with genetically modified dendritic cells was developed in order to induce T-cell immunity. We introduced the vector into dendritic cells as a plasmid DNA using polyethylenimine as the gene delivery system, thereby circumventing the problem of obtaining viral vector expression in the absence of integration. Genetically modified dendritic cells (GMDC) presented viral epitopes efficiently, secreted interleukin 12, and primed both CD4+ and CD8+ HIV-specific T cells capa

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Jenke, Andreas C. W., Andreas D. Wilhelm, Valerie Orth, Hans Joachim Lipps, Ulrike Protzer, and Stefan Wirth. "Long-Term Suppression of Hepatitis B Virus Replication by Short Hairpin RNA Expression Using the Scaffold/Matrix Attachment Region-Based Replicating Vector System pEPI-1." Antimicrobial Agents and Chemotherapy 52, no. 7 (2008): 2355–59. http://dx.doi.org/10.1128/aac.00067-08.

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ABSTRACT Since the emergence of viral resistance of hepatitis B virus (HBV) during treatment is becoming an important issue even with newer drugs, there is a need for alternative treatment options such as, for example, RNA interference (RNAi) technology. While short-term suppression of HBV replication is easily achieved with small interfering RNA oligonucleotides, this is not the case for long-term suppression due to the lack of an optimal vector system. Based on the nonviral scaffold/matrix attachment region (S/MAR)-based vector system pEPI-1, which is free of common side effects and is stabl

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Purwono, Purwono, Annastasya Nabila Elsa Wulandari, and Novieta Hardeani Sari. "Virus Host Prediction with Metagenomic Features using Support Vector Machine Algorithm and Grid Search Cross Validation Optimization." Journal of Advanced Health Informatics Research 2, no. 3 (2024): 127–37. https://doi.org/10.59247/jahir.v2i3.298.

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Viruses and bacteria continue to evolve alongside humans. Viruses are spreading too fast and causing a huge loss of life in the world. Viruses play an important role as dangerous pathogens that continue to spread various infectious diseases. Metegenomics is the application of large sequencing technology to genetic material obtained directly from one or more environmental samples, resulting in at least 50Mb random samples and multiple long sequences. It is important to identify the origin of the virus to prevent the spread of outbreaks. Understanding the biology of these viruses and how they af

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Evans, C. H. "ARTHRITIS GENE THERAPY: FROM CONCEPT TO CLINICAL TRIALS." Orthopaedic Proceedings 106-B, SUPP_1 (2024): 1. http://dx.doi.org/10.1302/1358-992x.2024.1.001.

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Intra-articular injection is a common way to deliver biologics to joints, but their effectiveness is limited by rapid clearance from the joint space. This barrier can be overcome by genetically modifying cells within the joint such that they produce anti-arthritic gene products endogenously, thereby achieving sustained, therapeutic, intra-articular concentrations of the transgene products without re-dosing. A variety of non-viral and viral vectors have been subjected to preclinical testing to evaluate their suitability for delivering genes to joints. The first transfer of a gene to a human joi

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Sayitoglu, Ece Canan, Didem Ozkazanc, Aydan Sarac, et al. "Identification and strategic targeting of key anti-viral defense mechanisms in Natural Killer cells." Journal of Immunology 200, no. 1_Supplement (2018): 169.19. http://dx.doi.org/10.4049/jimmunol.200.supp.169.19.

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Abstract While genetic modification of lymphocytes proves to be an efficient approach in immunotherapy, commonly used lentiviral vectors are haunted by the activity of intrinsic restriction factors in immune cells. Natural Killer (NK) cells are members of the innate immune system that possess strikingly high resistance to viral infections, presenting a challenge to virus-based gene delivery methods. In this study, high-throughput analytical approaches were used for comprehensive characterization of NK cell anti-viral defense mechanisms resulting in the observed resistance of NK cells to lentiv

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Stevanovic, Marta, Elena Piotter, Michelle E. McClements, and Robert E. MacLaren. "CRISPR Systems Suitable for Single AAV Vector Delivery." Current Gene Therapy 22, no. 1 (2022): 1–14. http://dx.doi.org/10.2174/1566523221666211006120355.

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Abstract: CRISPR (clustered regularly interspaced short palindromic repeats)/Cas gene editing is a revolutionary technology that can enable the correction of genetic mutations in vivo, providing great promise as a therapeutic intervention for inherited diseases. Adeno-associated viral (AAV) vectors are a potential vehicle for delivering CRISPR/Cas. However, they are restricted by their limited packaging capacity. Identifying smaller Cas orthologs that can be packaged, along with the required guide RNA elements, into a single AAV would be an important optimization for CRISPR/- Cas gene editing.

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McVey, Duncan, Mohammed Zuber, Douglas E. Brough, and Imre Kovesdi. "Adenovirus vector library: an approach to the discovery of gene and protein function." Journal of General Virology 84, no. 12 (2003): 3417–22. http://dx.doi.org/10.1099/vir.0.19446-0.

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A method was developed to generate a complex cDNA expression library within an adenovirus type 5 (Ad5)-based vector backbone, termed AdLibrary. Construction of the AdLibrary entailed the conversion of an Ad5 genome-containing cosmid to infectious virus particles. The Ad5 genome was modified by replacing the E1A and E1B genes with a Rous sarcoma virus-driven expression cassette. Conversion was accomplished by liberating the viral genome by restriction enzyme digestion and transfection in HEK 293 cells, which support the growth of E1A/E1B-deficient virus. A test AdLibrary demonstrated the possib

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Dohogne, Yael, Florence Collignon, Jean-Christophe Drugmand, et al. "Transferring Viral Vector Production from Plasticware to a Fixed-Bed Bioreactor." Genetic Engineering & Biotechnology News 39, no. 2 (2019): 60–62. http://dx.doi.org/10.1089/gen.39.02.19.

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Chaubal, Akshay S., and Andrew L. Zydney. "Single-Pass Tangential Flow Filtration (SPTFF) of Nanoparticles: Achieving Sustainable Operation with Dilute Colloidal Suspensions for Gene Therapy Applications." Membranes 13, no. 4 (2023): 433. http://dx.doi.org/10.3390/membranes13040433.

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Recent approval of several viral-vector-based therapeutics has led to renewed interest in the development of more efficient bioprocessing strategies for gene therapy products. Single-Pass Tangential Flow Filtration (SPTFF) can potentially provide inline concentration and final formulation of viral vectors with enhanced product quality due. In this study, SPTFF performance was evaluated using a suspension of 100 nm nanoparticles that mimics a typical lentivirus system. Data were obtained with flat-sheet cassettes having 300 kDa nominal molecular weight cutoff, either in full recirculation or si

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Dong, Chunyan, Zhuang Li, Dejiang Tan, et al. "Research and Clinical Progress of Therapeutic Tumor Vaccines." Vaccines 13, no. 7 (2025): 672. https://doi.org/10.3390/vaccines13070672.

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Therapeutic cancer vaccines are a new growth point of biomedicine with broad industrial prospects in the post-COVID-19 era. Many large international pharmaceutical companies and emerging biotechnology companies are deploying different tumor therapeutic cancer vaccine projects, focusing on promoting their clinical transformation, and the vaccine industry has strong momentum for development. Such vaccines are also the core engine and pilot site for the development of new vaccine targets, new vectors, new adjuvants, and new technologies, which play a key role in promoting the innovation and devel

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Gao, Jing, Lingxin Kong, Ruijie Luo, et al. "Research Progress of Virus-Induced Gene Silencing and its Application in Gramineae." Scholars Journal of Engineering and Technology 11, no. 07 (2023): 147–51. http://dx.doi.org/10.36347/sjet.2023.v11i07.001.

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With the proliferation of genome sequence information, biological science research has entered the era of big data, but how to annotate the function of genome information has become an important research goal. Virus-induced gene silencing (VIGS) is a powerful tool that has been used to study key genes in multiple plant growth and development processes. This technology uses the innate antiviral defense system of plants. And generates small interfering RNA (siRNA) in the plant by inserting the target gene fragment into the viral vector, so that the transcript of the endogenous gene of the plant

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