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Journal articles on the topic 'Virology – Technique'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

Fritz, Blayne, Stanley J. Naides, and Kenneth Moore. "A combined pseudoreplica-immunocytochemical technique for research and diagnostic virology." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 900–901. http://dx.doi.org/10.1017/s0424820100162065.

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The pseudoreplica method of staining viral particles for visualization by transmission electron microscopy is a very popular technique. The ability to concentrate clinical specimens while semi-embedding viral particles makes it especially well suited for morphologic and diagnostic virology. Immunolabelling viral particles with colloidal gold is a technique frequently employed by both research and diagnostic virologists. We have characterized a procedure which provides the advantage of both by modifying and combining pseudoreplica staining and immunogold labelling.Modification of specimen retri

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2

Davoodi, zohreh, jahangir heydarnejad, and Hossein Masoomi. "Next Generation Sequencing Technique and Its Application in Plant Virology." Plant Pathology Science 8, no. 2 (2019): 77–85. http://dx.doi.org/10.29252/pps.8.2.77.

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3

Fritz, Blayne, Kenneth Moore, and Stanley J. Naides. "A combined pseudoreplica-immunochemical technique for research and diagnostic Virology." Microscopy Research and Technique 21, no. 1 (1992): 59–64. http://dx.doi.org/10.1002/jemt.1070210109.

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4

Burton, Thomas D., and Nicholas S. Eyre. "Applications of Deep Mutational Scanning in Virology." Viruses 13, no. 6 (2021): 1020. http://dx.doi.org/10.3390/v13061020.

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Several recently developed high-throughput techniques have changed the field of molecular virology. For example, proteomics studies reveal complete interactomes of a viral protein, genome-wide CRISPR knockout and activation screens probe the importance of every single human gene in aiding or fighting a virus, and ChIP-seq experiments reveal genome-wide epigenetic changes in response to infection. Deep mutational scanning is a relatively novel form of protein science which allows the in-depth functional analysis of every nucleotide within a viral gene or genome, revealing regions of importance,

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5

Jerant-Patic, Vera. "Virology today and tomorrow." Srpski arhiv za celokupno lekarstvo 136, Suppl. 2 (2008): 171–81. http://dx.doi.org/10.2298/sarh08s2171j.

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Virus infections are the most common human diseases, particularly acute respiratory infections (mostly in children and young adults). Virus infections of the upper respiratory tract in children are proved in 77.4%, viral bronchitis in 58.6%, viral pneumonia in 47.6% and febrile conditions with lymphadenopathy in 44.1% patients. Current studies show that virus infections can cause not only acute, but also persistent virus diseases. A certain number of viruses are able to incorporate their nucleic acid into the genome of the host-cell, leading to rearrangement of the cell genes and formation of

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6

Basgall, Edward J., Gail Scherba, and Howard B. Gelberg. "Diagnostic virology in veterinary pathology: techniques for negative staining." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 366–67. http://dx.doi.org/10.1017/s0424820100103899.

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Negative staining of virus suspensions is the quickest, easiest diagnostic technique currently available to the electron microscopist. Virus sampling and staining from directly collected fluids is inherently rapid, simple, and straightforward. Virions in a sample can be stained directly on a formvar coated grid then examined in a transmission electron microscope (TEM).The collection, detection, and identification of enteric viruses is more involved. Intestinal contents are often presented in a variety of states, from clear fluid to solid. In a diagnostic setting, a routinely reliable protocol

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7

Panno, Stefano, Slavica Matić, Antonio Tiberini, et al. "Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology." Plants 9, no. 4 (2020): 461. http://dx.doi.org/10.3390/plants9040461.

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In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to seve

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8

Quemin, Emmanuelle R. J., Emily A. Machala, Benjamin Vollmer, et al. "Cellular Electron Cryo-Tomography to Study Virus-Host Interactions." Annual Review of Virology 7, no. 1 (2020): 239–62. http://dx.doi.org/10.1146/annurev-virology-021920-115935.

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Viruses are obligatory intracellular parasites that reprogram host cells upon infection to produce viral progeny. Here, we review recent structural insights into virus-host interactions in bacteria, archaea, and eukaryotes unveiled by cellular electron cryo-tomography (cryoET). This advanced three-dimensional imaging technique of vitreous samples in near-native state has matured over the past two decades and proven powerful in revealing molecular mechanisms underlying viral replication. Initial studies were restricted to cell peripheries and typically focused on early infection steps, analyzin

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9

Ho, W.-Z., E. Gönczöl, A. Srinivasan, S. D. Douglas, and S. A. Plotkin. "Minitransfection: a simple, fast technique for transfections." Journal of Virological Methods 32, no. 1 (1991): 79–88. http://dx.doi.org/10.1016/0166-0934(91)90187-5.

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10

Reuter, Jon D., Daniel Gomez, Janet L. Brandsma, John K. Rose, and Anjeanette Roberts. "Optimization of cottontail rabbit papilloma virus challenge technique." Journal of Virological Methods 98, no. 2 (2001): 127–34. http://dx.doi.org/10.1016/s0166-0934(01)00370-6.

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11

Garrett, A. J., and D. E. Warren. "A simple technique for endonuclease mapping of cytomegaloviruses." Journal of Virological Methods 10, no. 3 (1985): 187–94. http://dx.doi.org/10.1016/0166-0934(85)90059-x.

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12

Wagih, Elsayed, Hassan Melouk, and John Sherwood. "Mirror image in vivo electroblotting technique, a new technique for visualizing virus particles electrophoretically transferred from infected leaves to nitrocellulose membranes." Journal of Virological Methods 48, no. 2-3 (1994): 145–53. http://dx.doi.org/10.1016/0166-0934(94)90114-7.

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13

Mah Lee Ng, Foong May Yeong, and Suat Hoon Tan. "Cryosubstitution technique reveals new morphology of flavivirus-induced structures." Journal of Virological Methods 49, no. 3 (1994): 305–14. http://dx.doi.org/10.1016/0166-0934(94)90145-7.

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14

McCausland, Megan M., and Shane Crotty. "Quantitative PCR technique for detecting lymphocytic choriomeningitis virus in vivo." Journal of Virological Methods 147, no. 1 (2008): 167–76. http://dx.doi.org/10.1016/j.jviromet.2007.08.025.

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15

Chomel, J. J., Y. Robin, R. Durdilly, D. Thouvenot, M. Langlois, and M. Aymard. "Rapid direct diagnosis of mumps meningitis by ELISA capture technique." Journal of Virological Methods 68, no. 1 (1997): 97–104. http://dx.doi.org/10.1016/s0166-0934(97)00113-4.

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16

Bourinbaiar, Aldar S., Vanaja R. Zacharopoulos, and David M. Phillips. "Microwave irradiation-accelerated in situ hybridization technique for HIV detection." Journal of Virological Methods 35, no. 1 (1991): 49–58. http://dx.doi.org/10.1016/0166-0934(91)90084-d.

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17

Miller, Sara E. "Negative Staining: a Valuable Technique for Studying Subcellular Components." Microscopy and Microanalysis 3, S2 (1997): 341–42. http://dx.doi.org/10.1017/s143192760000859x.

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Negative staining is the most frequently used procedure for preparing particulate specimens, e.g., cell organelles, macromolecules, and viruses, for electron microscopy (Figs. 1-4). The main advantage is that it is rapid, requiring only minutes of preparation time. Another is that it avoids some of the harsh chemicals, e.g., organic solvents, used in thin sectioning. Also, it does not require advanced technical skill. It is widely used in virology, both in classification of viruses as well as diagnosis of viral diseases. Notwithstanding the necessity for fairly high particle counts, virus iden

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18

Jääskeläinen, Anne J., and Leena Maunula. "Applicability of microarray technique for the detection of noro- and astroviruses." Journal of Virological Methods 136, no. 1-2 (2006): 210–16. http://dx.doi.org/10.1016/j.jviromet.2006.05.015.

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19

Boltovets, P. M., V. R. Boyko, I. Yu Kostikov, N. S. Dyachenko, B. A. Snopok, and Yu M. Shirshov. "Simple method for plant virus detection: effect of antibody immobilization technique." Journal of Virological Methods 105, no. 1 (2002): 141–46. http://dx.doi.org/10.1016/s0166-0934(02)00098-8.

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20

El-Ghorr, A. A., D. R. Snodgrass, and F. M. M. Scott. "Evaluation of an immunogold electron microscopy technique for detecting bovine coronavirus." Journal of Virological Methods 19, no. 3-4 (1988): 215–23. http://dx.doi.org/10.1016/0166-0934(88)90016-x.

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21

van der Heijden, Mitzi, Michel de Vries, Frank G. van Steenbeek, et al. "Sequence-independent VIDISCA-454 technique to discover new viruses in canine livers." Journal of Virological Methods 185, no. 1 (2012): 152–55. http://dx.doi.org/10.1016/j.jviromet.2012.05.019.

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22

Zou, Xiao-Hui, Zhi-Xiang Bi, Xiao-Juan Guo, et al. "☆DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus." Journal of Virological Methods 257 (July 2018): 85–92. http://dx.doi.org/10.1016/j.jviromet.2018.04.001.

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23

Killough, Rosemary, Paul E. Klapper, Andrew S. Bailey, Ian R. Sharp, A. Tullo, and Shiriey J. Richmond. "An immune dot-blot technique for the diagnosis of ocular adenovirus infection." Journal of Virological Methods 30, no. 2 (1990): 197–203. http://dx.doi.org/10.1016/0166-0934(90)90020-g.

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24

Vorndam, Vance, Goro Kuno, and Noemí Rosado. "A PCR-restriction enzyme technique for determining dengue virus subgroups within serotypes." Journal of Virological Methods 48, no. 2-3 (1994): 237–44. http://dx.doi.org/10.1016/0166-0934(94)90122-8.

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25

Calicó, I., E. Balada, A. Cortés Borra, and E. Mercader. "A simplified technique for determining the sensitivity of cytomegalovirus strains to ganciclovir." Journal of Virological Methods 60, no. 1 (1996): 59–64. http://dx.doi.org/10.1016/0166-0934(96)02045-9.

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26

Meents, A., and M. O. Wiedorn. "Virus Structures by X-Ray Free-Electron Lasers." Annual Review of Virology 6, no. 1 (2019): 161–76. http://dx.doi.org/10.1146/annurev-virology-092818-015724.

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Until recently X-ray crystallography has been the standard technique for virus structure determinations. Available X-ray sources have continuously improved over the decades, leading to the realization of X-ray free-electron lasers (XFELs). They provide high-intensity femtosecond X-ray pulses, which allow for new kinds of experiments by making use of the diffraction-before-destruction principle. By overcoming classical dose constraints, they at least in principle allow researchers to perform X-ray virus structure determination for single particles at room temperature. Simultaneously, the availa

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27

Åsjö, B., J. Albert, F. Chiodi, and E. M. Fenyö. "Improved tissue culture technique for production of poorly replicating human immunodeficiency virus strains." Journal of Virological Methods 19, no. 3-4 (1988): 191–96. http://dx.doi.org/10.1016/0166-0934(88)90013-4.

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28

Lee, Spencer H. S., James E. Boutilier, Margaret A. MacDonald, and Kevin R. Forward. "Enhanced detection of respiratory viruses using the shell vial technique and monoclonal antibodies." Journal of Virological Methods 39, no. 1-2 (1992): 39–46. http://dx.doi.org/10.1016/0166-0934(92)90123-u.

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29

Ushijima, Hiroshi, Tatsuhiko Shinozaki, and Takashi Kitamura. "Detection of anti rotavirus coproantibodies by immunoblotting technique." Journal of Medical Virology 29, no. 2 (1989): 127–29. http://dx.doi.org/10.1002/jmv.1890290209.

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30

Aspholm, Robert, Shusheng Zuo, Jan Fohlman, Gun Frisk, Göran Friman, and Jonas Blomberg. "A novel serological technique: polymerase chain reaction enhanced immunoassay. Application to enterovirus IgM diagnosis." Journal of Virological Methods 80, no. 2 (1999): 187–96. http://dx.doi.org/10.1016/s0166-0934(99)00046-4.

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31

Cormier, Jiemin, and Marlene Janes. "A double layer plaque assay using spread plate technique for enumeration of bacteriophage MS2." Journal of Virological Methods 196 (February 2014): 86–92. http://dx.doi.org/10.1016/j.jviromet.2013.10.034.

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32

Moench, T. "Efficiency of in situ hybridization as a function of probe size and fixation technique." Journal of Virological Methods 11, no. 2 (1985): 119–30. http://dx.doi.org/10.1016/0166-0934(85)90035-7.

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33

Codd, A. A., and H. K. Narang. "An ion-exchange capture technique for routine identification of faecal viruses by electron microscopy." Journal of Virological Methods 14, no. 3-4 (1986): 229–35. http://dx.doi.org/10.1016/0166-0934(86)90024-8.

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34

Graddon, D. J., and J. W. Randles. "Single antibody dot immunoassay — A simple technique for rapid detection of a plant virus." Journal of Virological Methods 13, no. 1 (1986): 63–69. http://dx.doi.org/10.1016/0166-0934(86)90073-x.

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35

Bansal, R. P., B. Sharma, R. C. Joshi, and U. Chandra. "Application of solid phase aggregation of coated erythrocytes technique for detection of rinderpest antigen." Journal of Virological Methods 16, no. 4 (1987): 317–21. http://dx.doi.org/10.1016/0166-0934(87)90016-4.

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36

Dahling, Daniel R., and Betty A. Wright. "Optimization of suspended cell method and comparison with cell monolayer technique for virus assays." Journal of Virological Methods 20, no. 2 (1988): 169–79. http://dx.doi.org/10.1016/0166-0934(88)90150-4.

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37

Romano, Joseph W., Roxanne N. Shurtliff, M. G. Sarngadharan, and Ranajit Pal. "Detection of HIV-1 infection in vitro using NASBA: an isothermal RNA amplification technique." Journal of Virological Methods 54, no. 2-3 (1995): 109–19. http://dx.doi.org/10.1016/0166-0934(95)00031-o.

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38

Brockmann, Dieter, Barbara Tries, and Helmut Esche. "Isolation and characterization of novel adenovirus type 12 ElA mRNAs by cDNA PCR technique." Virology 179, no. 2 (1990): 585–90. http://dx.doi.org/10.1016/0042-6822(90)90125-b.

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39

Mempel, Martin, Bernd Günther, Edgar Müller, and Ludwig Grüter. "Detection and Measurement ofStaphylococcus epidermidisSlime Using an ELISA Technique." Microbiology and Immunology 40, no. 2 (1996): 115–19. http://dx.doi.org/10.1111/j.1348-0421.1996.tb03325.x.

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40

Wong, Anna J., Denny G. Constantine, Oscar Armstrong, Wanda Y. Wong, and Jean C. Comb. "A novel technique to eliminate cross-contamination when making wells on slides for rabies diagnosis." Journal of Virological Methods 115, no. 2 (2004): 117–22. http://dx.doi.org/10.1016/j.jviromet.2003.09.019.

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41

Kotecha, Abhay, Fuquan Zhang, Nicholas Juleff, et al. "Application of the thermofluor PaSTRy technique for improving foot-and-mouth disease virus vaccine formulation." Journal of General Virology 97, no. 7 (2016): 1557–65. http://dx.doi.org/10.1099/jgv.0.000462.

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42

V., Pavana Jyothi, Akila S., Malini K. Selvan, et al. "Direct typing of Canine parvovirus (CPV) from infected dog faeces by rapid mini sequencing technique." Journal of Virological Methods 238 (December 2016): 66–69. http://dx.doi.org/10.1016/j.jviromet.2016.09.012.

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43

Parkkinen, Sinikka. "Nucleic acid sandwich hybridization in detection of HPV 16 DNA: Technique and its clinical application." Journal of Virological Methods 19, no. 1 (1988): 69–77. http://dx.doi.org/10.1016/0166-0934(88)90008-0.

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44

Rawlinson, W. D., D. E. Dwyer, V. L. Gibbons, and A. L. Cunningham. "Rapid diagnosis of varicella-zoster virus infection with a monoclonal antibody based direct immunofluorescence technique." Journal of Virological Methods 23, no. 1 (1989): 13–18. http://dx.doi.org/10.1016/0166-0934(89)90084-0.

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45

Garnier, M., T. Candresse, and J. M. Bove. "Immunocytochemical localization of TYMV-coded structural and nonstructural proteins by the protein A-gold technique." Virology 151, no. 1 (1986): 100–109. http://dx.doi.org/10.1016/0042-6822(86)90107-8.

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46

Wang, Yunpeng. "Comparative study of different detection method on the virus titers." Journal of Applied Virology 2, no. 3 (2013): 6. http://dx.doi.org/10.21092/jav.v2i3.19.

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<p>The counting of the virions plays a significant role in the study of virology. The detection methods of the virus titers can be mainly divided into two kinds: that based on the experimental animals and that on the cell culture technique. The former can be applied to those viruses that possess the experimental animal model, and the latter, according to the pathological advances of the cells, can be further divided into direct observation of the pathological advances and the plaque detection. When it comes to those viruses, that either have no CPE symptom after having infected the cells

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47

Pawlotsky, Jean-Michel, Anne Bastie, Isabelle Lonjon, et al. "What technique should be used for routine detection and quantification of HBV DNA in clinical samples?" Journal of Virological Methods 65, no. 2 (1997): 245–53. http://dx.doi.org/10.1016/s0166-0934(97)02196-4.

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48

Chueh, Ling-Ling, Kan-Hung Lee, Chian-Ren Jeng, and Victor Fei Pang. "A sensitive fluorescence in situ hybridization technique for detection of porcine reproductive and respiratory syndrome virus." Journal of Virological Methods 79, no. 2 (1999): 133–40. http://dx.doi.org/10.1016/s0166-0934(99)00004-x.

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49

Cha, Seho, Jun Hyeong Jang, Yejin Kim, Lee Rang Hwang, and Taegun Seo. "Analysis of Kaposi's sarcoma-associated herpesvirus latent replication using a real-time polymerase chain reaction technique." Journal of Virological Methods 193, no. 2 (2013): 660–66. http://dx.doi.org/10.1016/j.jviromet.2013.07.061.

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50

Gibson, C. A., J. M. Wood, Jennifer Mumford, G. C. Schild, and Averil M. Bevan. "A single-radial haemolysis technique for measurement of antibody to influenza virus neuraminidase in equine sera." Journal of Virological Methods 11, no. 4 (1985): 299–308. http://dx.doi.org/10.1016/0166-0934(85)90023-0.

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