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Dissertations / Theses on the topic 'Virus detection method'

Author: Grafiati

Published: 28 May 2022

Last updated: 31 July 2025

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1

Milne, Sarah Amelia. "The development of a sensitive and reliable molecular method for the detection of human pathogenic viruses in bivalve molluscs." Thesis, University of Aberdeen, 2002. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU159700.

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The overall aim of this project was to develop a sensitive, specific and reliable molecular assay for the detection of human pathogenic viruses in shellfish. In initial studies, 'viral surrogates' were used to evaluate two different assay formats, the reverse transcriptase-polymerase chain reaction-enzyme-linked immunosorbent assay (RT-PCR-ELISA) and the enzyme-labelled deoxynucleic acid-enzyme-linked coagulation assay (EDNA-ELCA). Each format was tested for ease of use, reliability and sensitivity, when compared with ethidium bromide gel detection. The RT-PCR-ELISA proved to be a successful a

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2

Gallegos, Karen M. "Characterization of Pathogens for Potential Diagnostic Tests." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1367942509.

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3

PANDIAN, ARIVAZHAGAN. "Optimization of the Information Collection Rule’s Adsorption/Elution Method for Virus Detection and Enumeration." University of Cincinnati / OhioLINK, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=ucin975357716.

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4

Cha, Wonhee. "Application of multiplex branched DNA method for the detection and study of avian inlfuenza [i.e. influenza] virus." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1211475165.

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5

Walsh, Helen Ann. "Rapid Detection of Grapevine Leafroll-associated virus Type 3 using the reverse transcription loop-mediated amplification method." Diss., University of Pretoria, 2013. http://hdl.handle.net/2263/41018.

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Grapevine Leafroll disease (GLD), one of the most destructive diseases of grapevines, has been found in every country where grapevines are grown. Grapevine Leafroll associated virus type 3 (GLRaV-3), one of several viruses associated with GLD globally, is the most prevalent virus in South African grapevines and therefore control of GLRaV-3 takes high priority in any strategy aimed at control of GLD. GLD can be controlled through the use of an integrated strategy which includes using certified plant material, controlling insect vectors through use of systemic insecticides and the removal

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6

Payne, Holly Ann. "DEVELOPMENT OF A METHOD FOR POLIOVIRUS DETECTION IN FRESHWATER CLAMS (COLORADO RIVER, CORBICULA FLUMINEZ)." Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275315.

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7

Musial, Cora Estabrook. "Development of a method for the detection of Cryptosporidium in water and selected studies on hepatitis A virus." Diss., The University of Arizona, 1985. http://etd.library.arizona.edu/etd/GetFileServlet?file=file:///data1/pdf/etd/azu_e9791_1985_343_sip1_w.pdf&type=application/pdf.

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8

Plante-Driscoll, Michelle L. "Development of a novel method for the rapid concentration and detection of norovirus and hepatitis A virus in foods." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/27604.

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Noroviruses (NoV) and Hepatitis A viruses (HAV) are the most commonly implicated viruses in foodborne disease. Their transmission is mainly via the fecal-oral route and distribution of contaminated foods has lead to large outbreaks. Thus, it is crucial that contaminated foods be identified promptly. Detection by cell culture is not possible for these viruses so that researchers rely on the reverse transcription polymerase chain reaction (RT-PCR). Prior to detection, however, viruses must be isolated from foods and, as of yet, no one method has been found applicable to a large variety of food m

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9

Lemaire, Olivier. "Contribution a l'etude des proprietes biologiques des rna du virus de la rhizomanie (beet necrotic yellow vein virus) et de leur role dans l'etiologie de la maladie." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13115.

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10

BERNA, ANNE. "Les proteines non structurales du virus de la mosaique de la luzerne : immunodetection et role possible dans la multiplication du virus." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13039.

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Utilisation de la technique des immunoempreintes pour detecter les 3 proteines non structurales du virus de la mosaique de la luzerne dans des tabacs infectes. Ces proteines se trouvent dans la fraction membranaire contenant le complexe de replication viral. Les proteines p1 et p2 ont la meme cinetique d'apparition que la replicase au cours de la virose. Il est possique que p2 soit la polymerase virale; p1 ne participerait qu'a la synthese des brins. P3 pourrait etre impliquee dans la diffusion systemique du virus

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11

Zarski, Jean-Pierre. "Virus de l'hépatite B : mise en évidence du génome viral et étude des mutations pré-C." Université Joseph Fourier (Grenoble ; 1971-2015), 1996. http://www.theses.fr/1996GRE10217.

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Ce travail rapporte dans sa premiere partie, l'interet de la detection de l'adn du virus de l'hepatite b (vhb) dans le serum, par differentes methodes permettant une quantification de la viremie. Apres avoir mis au point une technique d'hybridation en phase liquide avec marquage a l'iode 131, l'auteur montre son interet dans le suivi des malades sous traitement antiviral. La technique de pcr est plus sensible et permet dans certains cas de differencier les vrais des faux-positifs. La technique d'hybride capture avec revelation en chemiluminescence, a une sensibilite equivalente et est plus fac

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12

DORE, PETIT-MAIRE ISABELLE. "Utilisation des anticorps monoclonaux en virologie vegetale : diagnostic et etudes structurales de quelques tobamovirus." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13097.

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Etude des epitopes reconnus par 18 anticorps monoclonaux diriges contre la proteine de l'enveloppe du virus de la mosaique du tabac, en mesurant leur reactivite vis-a-vis de differents mutants, virus et peptides synthetiques. Visualisation de la liaison entre anticorps monoclonaux et virus par microscopie electronique, dans differents tests elisa. Preparation d'anticorps monoclonaux permettant la detection du virus des taches annulaires de l'odontoglossum et du virus de la mosaique de la tomate

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13

Чумаченко, К. И. "Классификация вредоносного программного обеспечения с помощью методов машинного обучения". Thesis, Сумский государственный университет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/64341.

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В данной работе было проведено исследование, направленное на определение наиболее точного метода обнаружения вирусов, представленного в виде задач бинарной классификации (вирус / легитимная программа) и многоклассовой классификации. В качестве признаков были использованы успешные и безуспешные вызовы API, а также соответствующие коды возврата, которые были получены во время выполнения вирусов в изолированной виртуальной среде.

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Darwaish, Asim. "Adversary-aware machine learning models for malware detection systems." Electronic Thesis or Diss., Université Paris Cité, 2022. http://www.theses.fr/2022UNIP7283.

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La popularisation des smartphones et leur caractère indispensable les rendent aujourd'hui indéniables. Leur croissance exponentielle est également à l'origine de l'apparition de nombreux logiciels malveillants et fait trembler le prospère écosystème mobile. Parmi tous les systèmes d'exploitation des smartphones, Android est le plus ciblé par les auteurs de logiciels malveillants en raison de sa popularité, de sa disponibilité en tant que logiciel libre, et de sa capacité intrinsèque à accéder aux ressources internes. Les approches basées sur l'apprentissage automatique ont été déployées avec s

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15

Albrecht, Huguette. "Les produits des genes i et iv du virus de la mosaique du chou-fleur (camv)." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13178.

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Bajalan, Amanj. "Improved methods for virus detection and discovery in metagenomic sequence data." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-412478.

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17

Kienlen, Laura L. "Comparison of bioaerosol collection methods in the detection of airborne influenza virus." Thesis, University of Iowa, 2015. https://ir.uiowa.edu/etd/1658.

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Detection of airborne influenza virus is needed in order to determine exposure and prevent and control infections. Few researchers have successfully detected airborne influenza virus in environmental settings with current bioaerosol samplers. Therefore, new sampling strategies should be considered to increase the likelihood of detection. This study compared four bioaerosol samplers in collection of airborne influenza virus – the SKC Biosampler, NIOSH Biosampler, Andersen N6 single-stage impactor containing a liquid media, and the newly developed Next Generation Inhalable Aerosol Sampler (NGIAS

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18

McNaughton, Anna Lukats. "Mixed genotype hepatitis C virus infections : incidence in Scotland and methods for detection." Thesis, University of Glasgow, 2017. http://theses.gla.ac.uk/7848/.

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The diagnosis of mixed genotype hepatitis C virus (HCV) infection is rare and information on incidence in the UK, where genotypes 1a and 3 are the most prevalent, is sparse. Considerable variations in the efficacies of direct-acting antivirals (DAAs) for the HCV genotypes have been documented and the ability of DAAs to treat mixed genotype HCV infections remains unclear, with the possibility that genotype switching may occur. In order to estimate the prevalence of mixed genotype 1a/3 infections in Scotland, a cohort of 512 samples was compiled and then screened using a genotype-specific nested

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19

Elfaitouri, Amal. "Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7320.

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20

Wong, Pik-wa Linda, and 黃碧華. "Optimization of detection of avian influenza virus in formalin fixed tissues by immunohistochemical methods." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42905333.

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Wong, Pik-wa Linda. "Optimization of detection of avian influenza virus in formalin fixed tissues by immunohistochemical methods." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42905333.

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22

McKie, Anne. "Immunity surveillance of mumps and rubella : improved methods for the detection of virus-specific antibody." Thesis, University of Greenwich, 2003. http://gala.gre.ac.uk/6247/.

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The aim of these studies was to improve laboratory methods for the detection of virus-specific antibody to mumps and rubella. The presence of virus-specific antibody is indicative of immunity to disease so simple and effective antibody detection allows for the planning and monitoring of immunisation programmes. In facilitating antibody surveillance, oral fluid has advantages as a sample compared with blood. It is simple, safe and cheap to collect and being non-invasive encourages subject recruitment. In this study, an ‘IgG’ antibody capture enzyme-linked immunosorbent assay (GACELISA) was deve

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23

Felice, Viviana <1986&gt. "Insights on molecular characterization and on methods of detection of Infectious Bursal Disease virus and Avian Mycoplasmas." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2019. http://amsdottorato.unibo.it/8854/1/Felice_Viviana_Tesi.pdf.

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Among the diseases that threaten the health of poultry, immunosuppressive and respiratory infections play a major role. The first part of this thesis focuses on the phylogenetic analysis of an emerging genotype of the Infectious Bursal Disease virus (IBDV), denominated ITA. Sequence alignments revealed that the virus shared residues with either very virulent IBDVs or low-virulence strains, confirming its distinctive genetic characteristics. Moreover, no evidences of recombination and reassortant phenomena were found in the ITA genotype. Substantial economic losses are caused in poultry by

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24

BIBEAU, FREDERIC. "Analyse comparative des methodes de detection du virus d'epstein-barr dans la maladie de hodgkin : etude de l'expression des genes viraux." Toulouse 3, 1994. http://www.theses.fr/1994TOU31522.

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25

Thedell, T. Aron. "Comparisons of a novel industrial hygiene filter material, wash solutions, and extraction methods in the detection and quantification of influenza virus." Thesis, University of Iowa, 2017. https://ir.uiowa.edu/etd/5862.

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The collection and quantification of influenza virus is important in determining exposure, and in the control of infection. There are a number of bioaerosol samplers and analytical methods available to do this, however few researchers have been able to detect influenza virus in the environment. Therefore, new samplers and methods should be considered. This study compared influenza virus recovery from three filter types (PTFE, PVC, and PS) treated with sampling air or no air, using HBSS or PBS as wash buffers, and the QIAamp Viral RNA Mini Kit or Trizol method in the extraction of viral RNA. Tw

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26

Dekker, Elise. "Utilisation des anticorps monoclonaux pour l'etude de quelques tobamovirus et geminivirus." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13143.

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27

Чумаченко, К. И. "Классификация вредоносного программного обеспечения с помощью методов машинного обучения". Thesis, Сумский государственный университет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/65587.

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В данной работе было проведено исследование, направленное на определение наиболее точного метода обнаружения вирусов, представленного в виде задач бинарной классификации (вирус / легитимная программа) и многоклассовой классификации. В качестве признаков были использованы успешные и безуспешные вызовы API, а также соответствующие коды возврата, которые были получены во время выполнения вирусов в изолированной виртуальной среде.

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28

Chiou, Shih-Hao, and 邱士豪. "Development of Rapid Detection Method for Cymbidium Mosaic Virus." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/07778023135734307457.

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碩士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>95<br>Phalaenopsis sp., an ornamental crop belonging to the Orchidacea, is an important commercial crop in the floral industry. Taiwan is one of the biggest exporters of Phalaenopsis orchid in the world market. Cymbidium Mosaic virus（CymMV）is one of the most economically important virus of Phalaenopsis orchid. The yield and quality of orchid production are influenced by this virus. To solve this problem, the virus-free orchids that used for breeding and the management of viral disease in orchid nurseries need to be developed. Therefore, a reliable and effective

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Lu, Yu-Tsung, and 呂侑璁. "Development of A Sensitive Method for Novel Virus Detection." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/72653987402736388785.

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碩士<br>國立臺灣大學<br>醫學檢驗暨生物技術學研究所<br>97<br>In the past decades, the infections caused by newly emerging viruses increased rapidly. For the specimens containing potential unknown pathogens, in addition to the electron microscopy examination, microbe-microarray hybridization is usually used to identify the causative pathogens. To further increase the detection rate, several pre-analysis amplification methods were included for amplification of the nucleic acids of causative pathogens, such as the representational difference analysis (RDA), sequence-independent single primer amplification (SISPA), or

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Larsen, Sophie. "Development of a Method for the Detection of Aleutian Mink Disease Virus in Water Samples." 2013. http://hdl.handle.net/10222/21457.

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Aleutian mink disease virus (AMDV) causes significant loss to the mink industry in Nova Scotia (NS). Contaminated water is a speculated virus source therefore my objective was to develop a method for detection of AMDV in water samples. Initially, the quality and quantity of AMDV DNA extracted by four commercial kits was evaluated by PCR. Next, viral capture by adsorption-elution filtration was tested using spiked water samples. The 1MDS filter method was superior or comparable to the MgCl2 filter method and outperformed the AlCl3 method. Lastly, 16 watershed samples from Southwest NS and 11 sa

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Zong-HanLi and 李宗翰. "Development of a serological detection method for hepatitis B virus large surface antigen." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/c56h75.

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碩士<br>國立成功大學<br>醫學檢驗生物技術學系碩博士班<br>100<br>Hepatitis B virus (HBV) infection is the primary cause of hepatocellular carcinoma (HCC) in Taiwan. Since HBV may persist for decades as chronic infection before hepatic carcinogenesis. It is important to evaluate the HBV infection phases and monitor the risks of HCC carcinogenesis. According to previous studies, a pathological symptom of ground glass hepatocytes (GGHs) was identified to express different morphology of HBV surface antigens (HBS) in chronic HBV infection patients and HCC cases. Due to the pre-S region mutations that GGHs were primary cla

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Lin, Yu-Hsuan, and 林育萱. "Strain-differentiation of Citrus tatter leaf virus (CTLV) and development of a rapid method for CTLV detection." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/87337531614154677992.

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碩士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>92<br>Citrus tatter leaf disease is one of the important systemic diseases of citrus. It is caused by Citrus tatter leaf virus (CTLV), which is categorized into the genus Capillovirus consisting of a single-stranded, positive-sense RNA genome packaged in circa 650 nm flexuous particles. CTLV is transmitted by vegetative propagation and / or mechanical inoculation. The virus is usually latent in most commercialized citrus cultivars, but it induces dwarfing, distortion, mottling and “tatter leaf” in the infected leaves of susceptible citrus hosts such as Kalpi lim

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Chang, Chia-yang, and 張嘉洋. "Development of the immunological detection method for Macrobrachium rosenbergii nodavirus and extra small virus using recombinant protein preparation." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/80332154760065233982.

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碩士<br>國立高雄大學<br>生物科技研究所<br>99<br>Postlarvae of Macrobrachium rosenbergii infected with white tail disease (WTD) were previously reported in Taiwan. The causative agents have been identified as Macrobrachium rosenbergii nodavirus (MrNV) associated with extra small virus (XSV). In this study, the gene sequence encoding the capsid protein of MrNV and XSV were separately cloned into pGEX-4T-3 expression vector and transformed into Escherichia coli BL-21 strain. After induction, glutathione-S-transferase (GST) tagged MrNV and XSV were obtained with molecular mass of 68 and 43 KDa, respectively.

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Wu, Chung-Wei, and 吳崇維. "Detection of Human papillomavirus and Epstein- Bar virus in oral squamous cell carcinoma by using the polymerase chain reaction method." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/99136747208973921734.

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碩士<br>高雄醫學大學<br>牙醫學研究所<br>88<br>The purpose of this study was to understand the relationship between 50 specimens of oral squamous cell carcinoma or 20 specimens of gingiva and HPV-16 and EBV infection by using polymerase chain reaction method. Our results showed that HPV-16 infection rate of normal gingiva was 10%, and the EBV infection rate is also 10%. The oral cancer patients were divided into one group with betel nut chewing habit and the other group without betel nut chewing habit. HPV-16 DNA could be identified in 15 out of 44 (34%) oral cancer specimens from patients with betel nut che

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Wu, Hsin-huan, and 吳興煥. "Evaluation of a method for RT-PCR detection of cymbidium mosaic virus without RNA extraction and phylogenetic analysis of CyMV strain-type in Taiwan." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/apt28v.

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碩士<br>東吳大學<br>微生物學系<br>93<br>Phalaenopsis is one of the most important ornamental crop in Taiwan. Cymbidium mosaic virus (CyMV) is the economically most important virus that affects the health and quality of orchid plants. The key point of health inspection and quarantine is a fast and highly sensitive method for the diagnosis of virus. The objective of this study was to establish a rapid, simple and sensitive one-step RT-PCR method that detects CyMV without nucleic acid isolation. Lysine inhibits the activity of DNase in the sap for detection of CyMV by two steps RT-PCR. Leaf sap of infested

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Huang, Sz-Jia, and 黃偲佳. "Development of a rapid detection method for Banana streak virus and the survey of banana streak disease on Musa spp. germplasm collection in Taiwan." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/71797671388462843330.

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碩士<br>臺灣大學<br>植物病理與微生物學研究所<br>98<br>BSV belonging to the genus Badnavirus in the family Caulimoviridae, is a double-stranded DNA virus. Part of the BSV genome usually becomes endogenous pararetroviral sequences (EPRVs) that can be found in all banana varieties containing genome derived from Musa balbisiana. It indicated an integration event happened in Musa balbisiana. EPRV does not cause symptoms on bananas, but it will sometimes become active (episomal form) during vegetative propagation such as tissue culture and cause severe symptoms on plants. Currently 6 different strains of BSV have bee

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Huang, Pei-Ling, and 黃佩玲. "The detection of Apple chlorotic leaf spot virus from imported pear scions by the method of reverse transcription loop-mediated isothermal amplification (RT-LAMP)." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/g7nn9p.

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碩士<br>國立臺灣大學<br>植物醫學碩士學位學程<br>107<br>To produce high quality pear, branches with flower buds of pear grown in temperate region are imported from Japan and China to serves as scions to suckers of Hengshan pear in Taiwan. However, an important plant virus, Apple chlorotic leaf spot virus (ACLSV), commonly infects Rosaceae fruit trees and causes economic damages in foreign countries. Therefore, ACLSV has been listed as a quarantine pathogen. Currently, the detection of ACLSV is mainly conducted by use of enzyme-linked immunesorbent assay (ELISA) or reverse transcription polymerase chain reaction

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Wei-ShuoLing and 凌偉碩. "A pilot study of virus detection methods." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/74022229591007286534.

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碩士<br>國立成功大學<br>分子醫學研究所<br>101<br>Human immunodeficiency virus (HIV) has resulted in more than 25 million deaths worldwide since its discovery. Currently, approximately 34 million people are living with AIDS (UNAIDS, 2011 Global Report). To effectively track the disease, tools developed for highly sensitive HIV detection that are rapid, cost-friendly, and static of accuracy in diagnosing HIV early infection and screening of clinical/donor specimens are necessary. In this study, we developed two autocycling amplification-based detection methods (X and Y types) for detecting HIV-1 B subtype viru

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蔡雯莉. "Molecular characterization and development of detection methods for Glory lily mosaic virus." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/87000742219774924183.

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THIEN, NGUYEN PHUC, and 阮福善. "Assessment of the Methods for the Detection of Hop Stunt Virus and Yellow Speckle Virus Related Grapevine." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/9828xr.

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博士<br>大葉大學<br>環境工程學系碩士班<br>105<br>In this study a multiplexreverse-transcription polymerase chain reaction (mRT PCR) technique is used simultaneously to detect viroids in grapevine. Fifty grapevine leaf samples with yellowing or mosaic symptoms were collected from difference vineyards at Changhua County, Taiwan during May to June, 2015-2016. Specific primer pairs for the detection of Hop stunt viroid (HSVd), Australian grapevine viroid (AGVd), Grapevine yellow speckle viroid-1 (GYSVd-1), Grapevine yellow speckle viroid-2 (GYSVd-2) and Citrus exocortis viroid (CEVd) were selected from previous

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Lin, Da-Wei, and 林大為. "A study of two intrusion detection methods-from email virus detection to web content self-integrity measurement." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/9t5stc.

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博士<br>國立中央大學<br>資訊管理研究所<br>94<br>Security assurance is the basis for success on the Internet. Viruses and worms constitute a great threat. Many countermeasures have been applied to counteract these malicious threats. Signature-based detection method works well only for a known virus or worm. It is very difficult to defend against an unknown virus or worm. Anomaly detection has the potential to detect unknown attacks. In this thesis, we proposed an abnormal mail detection method based on user mailing behavior. In our observation, human communication would form many parties. This characteristic

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"Rapid and Cost-effective Virus Detection Methods using Molecular Sensors and Nano-devices." Doctoral diss., 2016. http://hdl.handle.net/2286/R.I.40268.

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abstract: Accurate virus detection is important for diagnosis in a timely manner to facilitate rapid interventions and treatments. RNA viruses affect an extensive amount of the world’s population, particularly in tropical countries where emerging infectious agents often arise. Current diagnostic methods have three main problems: they are time consuming, typically not field-portable, and expensive. My research goal is to develop rapid, field-portable and cost sensitive diagnostic methods for RNA viruses. Herein, two different approaches to detect RNA viruses were proposed: Conjugated gold nanop

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Munro, James Leslie. "The epidemiology of gill-associated virus in Penaeus monodon and the development of alternative detection methods." Thesis, 2006. https://researchonline.jcu.edu.au/1573/1/01front.pdf.

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The hypothesis for this project was that gill-associated virus (GAV) affects production of Penaeus monodon on Australian farms. The main objectives of the research were, firstly; to determine if there is a relationship between the degree of GAV infection and the production of P. monodon from the examined farms. Secondly, to develop low cost detection methods for GAV and by inference, yellow head virus (YHV) which are applicable to the prawn farming industry in both Australia and developing countries. The first objective was achieved by sampling a total of forty five prawn ponds from three pra

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Munro, James Leslie. "The epidemiology of gill-associated virus in Penaeus monodon and the development of alternative detection methods /." 2006. http://eprints.jcu.edu.au/1573.

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"Novel methods for specific detection and quantification of covalently closed circular DNA in sera and biopsies of hepatitis B patients." Thesis, 2011. http://library.cuhk.edu.hk/record=b6075203.

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In conclusion, two new methods of cccDNA quantitation were developed and validated. The two assays are complementary to each other and may be used in patients with extreme HBV DNA levels. These cccDNA assays should be further validated in larger studies and may become important tests for diagnostic, prognostic and treatment monitoring purposes.<br>Over 350 million people worldwide suffer from chronic hepatitis B virus (HBV) infection, which leads to many cases of cirrhosis and hepatocellular carcinoma. HBV covalently closed circular DNA (cccDNA) is a critical intracellular replicative intermed

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Wu, Hsuan-Yi, and 巫宣毅. "Development and Application of Highly Efficient Methods for Detection of Transgenic Papayas Resistant to Papaya ringspot virus." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/87731487733000917563.

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碩士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>95<br>This thesis was dedicated to develop various efficient methods with the DNA-based techniques for the identification of transgenic papayas resistant to PRSV. These developed methods contain (1) PCR detection：This was developed for detecting 35S promoter, PRSV coat protein gene, Nos terminator in transgenic papayas using our devised 35S-726, CP-739 and Nos-220 primer pairs. Nested-PCR assays were conducted using the 35S-348 and CP-372 primers to increase sensitivity. Both 35S-726 and Nos-220 primer pairs were used to perform multiplex PCR assays. Primer SC-6

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Lee, Shu-Chuan, and 李淑娟. "Development of detection methods for two important orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV), and construction and characterization of an ORSV infectious cDNA clone." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/06136206523460152237.

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博士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>96<br>Orchid is one of the most important commercial plants in Taiwan. Virus infection in the mass production of orchid may result in great economic loss. Detection and screening the virus infected plant will reduce the risk of mass production and increase the plant quality. Three detection methods were developed for the detection of two covalent and important orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). The first one was a multiplex RT-PCR method. Specific primers were designed based on the respective viral coat protei

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Lee, Shu-Chuan. "Development of detection methods for two important orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV), and construction and characterization of an ORSV infectious cDNA clone." 2008. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2605200817510100.

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