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Journal articles on the topic 'Virus detection methods'

Author: Grafiati

Published: 28 May 2022

Last updated: 31 July 2025

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1

Cubitt, D. "Molecular Methods for Virus Detection." Journal of Clinical Pathology 49, no. 2 (1996): 188. http://dx.doi.org/10.1136/jcp.49.2.188-b.

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2

Wiedbrauk, Danny L. "Molecular Methods for Virus Detection." Laboratory Medicine 23, no. 11 (1992): 737–42. http://dx.doi.org/10.1093/labmed/23.11.737.

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3

Kaul, Karen. "Molecular Methods for Virus Detection." American Journal of Clinical Pathology 104, no. 6 (1995): 702.1–702. http://dx.doi.org/10.1093/ajcp/104.6.702.

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4

Peterson, Powers, and Roger S. Keresztes. "Molecular Methods for Virus Detection." American Journal of Clinical Pathology 104, no. 6 (1995): 702.2–703. http://dx.doi.org/10.1093/ajcp/104.6.702a.

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5

Fink, C. G. "Molecular Methods for Virus Detection." Molecular Pathology 50, no. 2 (1997): 111. http://dx.doi.org/10.1136/mp.50.2.111-a.

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6

Abdellatif, Berkat. "Metamorphic computer virus detection by Case-Based Reasoning (CBR) methods." International Journal of Software Engineering & Applications (IJSEA) 2, no. 4 (2021): 1–10. https://doi.org/10.5281/zenodo.4730062.

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Metamorphic virus employs code obfuscation techniques to mutate itself. It absconds from signature based detection system by modifying internal structure without compromising original functionality. -In this paper, we propose a new method, for detecting metamorphic computer viruses, that is based on the technique of Case-Based Reasoning (CBR). In this method: -Can detect similar viruses with high probability. - The updating of the virus database is done automatically without connecting to the Internet. Whenever a new virus is detected, it will be automatically added to the database used by our

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7

Sharma, Pradakshina, Shariq Suleman, Asim Farooqui, et al. "Analytical methods for Ebola virus detection." Microchemical Journal 178 (July 2022): 107333. http://dx.doi.org/10.1016/j.microc.2022.107333.

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8

Takahara, Yusuke, Yuki Nakaya, Masato Yasuura, Hiroki Ashiba, Penmetcha K. R. Kumar, and Makoto Fujimaki. "Comparison of Influenza Virus Detection Methods." Sensors and Materials 31, no. 1 (2019): 79. http://dx.doi.org/10.18494/sam.2019.2062.

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9

Guliy, O. I., B. D. Zaitsev, O. S. Larionova, and I. A. Borodina. "Virus Detection Methods and Biosensor Technologies." Biophysics 64, no. 6 (2019): 890–97. http://dx.doi.org/10.1134/s0006350919060095.

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10

REBELO-de-ANDRADE, H., and M. C. ZAMBON. "Different diagnostic methods for detection of influenza epidemics." Epidemiology and Infection 124, no. 3 (2000): 515–22. http://dx.doi.org/10.1017/s0950268899003751.

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Linking continuous community-based morbidity recording of influenza-like illness (ILI) with virological sampling has consistently proved its value as one of the earliest indicators of circulating influenza activity. The clinical morbidity recording in the Portuguese national surveillance network, during a 7-year period, and the contribution of different diagnostic techniques, including virus isolation, multiplex RT–PCR, immunocapture enzyme linked immunoassay (EIA) and complement fixation tests (CFTs) for the detection of influenza in such a community-based setting is described and evaluated i

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11

Miao, Chun Yu, and Li Na Chen. "A Research of Virus Detection Combined Dynamic and Static Analysis Methods." Advanced Materials Research 187 (February 2011): 625–30. http://dx.doi.org/10.4028/www.scientific.net/amr.187.625.

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we present a virus detection system based on the D-S theory of evidence, in which the dynamic and static analysis methods are combined. The detection engine applies two types of classifier, support vector amchine and probabilistic neural network to detect the virus. For SVM classifier, we extract the feature vector by monitoring the samples. And the static feature of samples is used in the probabilistic neural network classifier. Finally, the D-S theory of evidence is used to combine the contribution of each individual classifier to give the final decision.experiments show the presented method

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12

Mammadov, P. "MODERN DIAGNOSTIC METHODS USED IN DETECTION OF CYTOMEGALOVIRUS." Danish scientific journal, no. 70 (March 27, 2023): 10–12. https://doi.org/10.5281/zenodo.7788709.

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<strong>Abstract</strong> Human cytomegalovirus (HCMV), a betaherpes virus, is a major cause of birth defects and an important pathogen in immunocompromised individuals. Clinical symptoms may appear after initial infection, reinfection, or reactivation. Human cytomegalovirus (HCMV) usually does not cause obvious symptoms. Modern diagnostic methods indicate that HCMV is a widespread opportunistic infection in fetuses, bone marrow transplant patients, and AIDS patients. In this article, we will review the diagnostic methods used for cytomeqalovirus.

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Hansen, A. J., R. Hamilton, R. Martin, and R. Stace-Smith. "IMPROVED METHODS FOR TREE FRUIT VIRUS DETECTION." Acta Horticulturae, no. 193 (November 1986): 229–32. http://dx.doi.org/10.17660/actahortic.1986.193.39.

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14

Modrof, J., A. Berting, and T. R. Kreil. "Parallel Evaluation of Broad Virus Detection Methods." PDA Journal of Pharmaceutical Science and Technology 68, no. 6 (2014): 572–78. http://dx.doi.org/10.5731/pdajpst.2014.01014.

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15

Yang, Kai-Hung, and Roger J. Narayan. "Analytical methods for detection of Zika virus." MRS Communications 7, no. 2 (2017): 121–30. http://dx.doi.org/10.1557/mrc.2017.20.

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16

Gentit, P. "Detection of Plum pox virus: biological methods." EPPO Bulletin 36, no. 2 (2006): 251–53. http://dx.doi.org/10.1111/j.1365-2338.2006.00982.x.

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17

Lino, Alexandra, Marita A. Cardoso, Helena M. R. Gonçalves, and Paula Martins-Lopes. "SARS-CoV-2 Detection Methods." Chemosensors 10, no. 6 (2022): 221. http://dx.doi.org/10.3390/chemosensors10060221.

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A fast and highly specific detection of COVID-19 infections is essential in managing the virus dissemination networks. The most relevant technologies developed for SARS-CoV-2 detection, along with their advantages and limitations, will be presented and fully explored. Additionally, some of the newest and emerging COVID-19 diagnosis tools, such as biosensing platforms, will also be introduced. Considering the extreme relevance that all these technologies assume in pandemic control, it is of the utmost relevance to have an intrinsic knowledge of the parameters that need to be taken into consider

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18

Gul, Ijaz, Changyue Liu, Xi Yuan, et al. "Current and Perspective Sensing Methods for Monkeypox Virus." Bioengineering 9, no. 10 (2022): 571. http://dx.doi.org/10.3390/bioengineering9100571.

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The outbreak of the monkeypox virus (MPXV) in non-endemic countries is an emerging global health threat and may have an economic impact if proactive actions are not taken. As shown by the COVID-19 pandemic, rapid, accurate, and cost-effective virus detection techniques play a pivotal role in disease diagnosis and control. Considering the sudden multicountry MPXV outbreak, a critical evaluation of the MPXV detection approaches would be a timely addition to the endeavors in progress for MPXV control and prevention. Herein, we evaluate the current MPXV detection methods, discuss their pros and co

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19

Simo, Fredy Brice Nemg, Felicity Jane Burt, and Nigel Aminake Makoah. "Chikungunya Virus Diagnosis: A Review of Current Antigen Detection Methods." Tropical Medicine and Infectious Disease 8, no. 7 (2023): 365. http://dx.doi.org/10.3390/tropicalmed8070365.

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Chikungunya is a mosquito-borne viral disease caused by the chikungunya virus (CHIKV). CHIKV is expanding at an alarming rate, potentially spreading and establishing endemicity in new areas where competent vectors are present. The dramatic spread of CHIKV in recent years highlights the urgent need to take precautionary measures and investigate options for control. It is crucial in developing nations where diagnostic tools are limited, and symptoms are similar to other prevalent diseases such as malaria and dengue. The most reliable method for diagnosing chikungunya virus is viral gene detectio

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Bao, Hongmei, Xiaoxiao Feng, Yong Ma, et al. "Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse Transcription–Loop-Mediated Isothermal Amplification Methods." Journal of Clinical Microbiology 53, no. 12 (2015): 3884–87. http://dx.doi.org/10.1128/jcm.02165-15.

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We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription–loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.

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Tarim, E. Alperay, Betul Karakuzu, Cemre Oksuz, et al. "Microfluidic-based virus detection methods for respiratory diseases." Emergent Materials 4, no. 1 (2021): 143–68. http://dx.doi.org/10.1007/s42247-021-00169-7.

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AbstractWith the recent SARS-CoV-2 outbreak, the importance of rapid and direct detection of respiratory disease viruses has been well recognized. The detection of these viruses with novel technologies is vital in timely prevention and treatment strategies for epidemics and pandemics. Respiratory viruses can be detected from saliva, swab samples, nasal fluid, and blood, and collected samples can be analyzed by various techniques. Conventional methods for virus detection are based on techniques relying on cell culture, antigen-antibody interactions, and nucleic acids. However, these methods req

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22

Reyes Negrete, Heidy Stefania, Álvaro Sebastián Ron Mora, and Gabriela Paola Valenzuela Sánchez. "Serological and molecular methods for the detection of hepatitis B virus infections." Salud, Ciencia y Tecnología 5 (January 1, 2025): 1221. https://doi.org/10.56294/saludcyt20251221.

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Introduction: the hepatitis B virus has the ability to severely infect the liver and cause both acute and chronic infections. It belongs to the Hepadnaviridae family, is composed of partially double-stranded DNA, and contains four open reading frames (ORFs): ORF S (surface), C (core), P (polymerase), and X (HBx). The diagnosis is primarily based on detecting the surface antigen (HBsAg) and human antibodies against these antigens through immunological methods. Similarly, molecular methods such as PCR, qPCR, and LAMP are currently used, offering higher sensitivity and specificity. Objective: to

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23

Stobnicka-Kupiec, Agata, and Rafał Górny. "Methods of virus detection in various work environment." Podstawy i Metody Oceny Środowiska Pracy 35, no. 3(97) (2018): 71–85. http://dx.doi.org/10.5604/01.3001.0012.4767.

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Viruses are harmful biological agents, which can pose a risk for workers from different occupational groups. Information regarding the occurrence of viruses in occupational environment is still scarce, primarily due to analytical difficulty of their routine detection. Hygiene control applied for microbiological contamination are not effective in case of viruses, so the occupational risk for workers exposed to biological agents is still underestimated. Such a state of affairs fundamentally precludes a proper management of occupational safety. The effectiveness of virus detection in the environm

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24

Byrnes, A. P., and H. Willkommen. "Breakout Session C Summary: Current Virus Detection Methods." PDA Journal of Pharmaceutical Science and Technology 65, no. 6 (2011): 660–62. http://dx.doi.org/10.5731/pdajpst.2011.00835.

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25

Dodd, Roger Y. "The human immunodeficiency virus and associated detection methods." Plasma Therapy and Transfusion Technology 9, no. 2 (1988): 151–59. http://dx.doi.org/10.1016/0278-6222(88)90048-4.

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26

Olesen, Ann Sofie, Thomas Bruun Rasmussen, Søren Saxmose Nielsen, et al. "A Multi-Laboratory Comparison of Methods for Detection and Quantification of African Swine Fever Virus." Pathogens 11, no. 3 (2022): 325. http://dx.doi.org/10.3390/pathogens11030325.

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African swine fever is a viral disease of the family Suidae. Methods to detect and quantify African swine fever virus (ASFV) include qPCR and virus infectivity assays. Individual laboratories often use in-house procedures for these assays, which can hamper the comparison of results. The objective of this study was to estimate the probability of ASFV detection using these assays, and to determine the inter-test correlations between results. This was achieved by testing a panel of 80 samples at three reference laboratories. Samples were analysed using nucleic acid extraction and qPCR, as well as

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27

Kalantar, Mahsa Kalantar, Ali Hossein Rezayan, and Hassan Hajghaseem. "Dengue Fever and Novel Detection Methods Based on Biosensors." Journal of Poultry Sciences and Avian Diseases 2, no. 4 (2024): 35–47. http://dx.doi.org/10.61838/kman.jpsad.2.4.6.

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Dengue fever, a mosquito-borne viral infection, poses a significant global health threat, and early diagnosis is crucial for effective disease management. Infection of humans usually occurs through infected Aedes mosquitoes such as Aedes aegypti or Aedes albopictus with the dengue fever virus. The spectrum of disease ranges from asymptomatic infection and mild febrile illness (dengue fever) to more serious symptoms such as dengue hemorrhagic fever and dengue shock syndrome. Dengue fever is characterized by severe headache, high fever, skin rash, muscle and joint pain, nausea, and vomiting. Den

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28

ZAINUDDIN, AHMAD ANWAR, ANIS NURASHIKIN NORDIN, and ROSMINAZUIN AB RAHIM. "RECENT TRENDS IN DENGUE DETECTION METHODS USING BIOSENSORS." IIUM Engineering Journal 19, no. 2 (2018): 134–53. http://dx.doi.org/10.31436/iiumej.v19i2.931.

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ABSTRACT: Dengue illness is an infectious tropical disease, transmitted by Aedes mosquitos, that poses a serious health threat to the tropical world. This disease causes widespread infection worldwide, with about 50 million cases of dengue occurring per annum out of which 500,000 recorded cases of dengue hemorrhagic fever and 22,000 deaths. Currently, there are no effective vaccines available to prevent the spread of the infection. Accurate and rapid laboratory diagnostic tests are required for early detection to reduce patient mortality rate. In this paper, common laboratory diagnosis methods

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29

Lu, Siming, Sha Lin, Hongrui Zhang, Liguo Liang, and Shien Shen. "Methods of Respiratory Virus Detection: Advances towards Point-of-Care for Early Intervention." Micromachines 12, no. 6 (2021): 697. http://dx.doi.org/10.3390/mi12060697.

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Respiratory viral infections threaten human life and inflict an enormous healthcare burden worldwide. Frequent monitoring of viral antibodies and viral load can effectively help to control the spread of the virus and make timely interventions. However, current methods for detecting viral load require dedicated personnel and are time-consuming. Additionally, COVID-19 detection is generally relied on an automated PCR analyzer, which is highly instrument-dependent and expensive. As such, emerging technologies in the development of respiratory viral load assays for point-of-care (POC) testing are

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Loeffelholz, Michael, and Tasnee Chonmaitree. "Advances in Diagnosis of Respiratory Virus Infections." International Journal of Microbiology 2010 (2010): 1–5. http://dx.doi.org/10.1155/2010/126049.

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The diagnosis of respiratory virus infections has evolved substantially in recent years, with the emergence of new pathogens and the development of novel detection methods. While recent advances have improved the sensitivity and turn-around time of diagnostic tests for respiratory viruses, they have also raised important issues such as cost, and the clinical significance of detecting multiple viruses in a single specimen by molecular methods. This article reviews recent advances in specimen collection and detection methods for diagnosis of respiratory virus infections, and discusses the perfor

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31

Ankur, Singh Bist*. "A WIDE SCALE SURVEY ON CLUSTERING TECHNIQUES FOR MALWARE DETECTION." INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & RESEARCH TECHNOLOGY 6, no. 4 (2017): 307–13. https://doi.org/10.5281/zenodo.546351.

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Use of internet has enhanced the problem of malwares. At the same time the advancement in technology has given rise to evolution of portable mobile devices. It has become the danger point of day today activities like mails, mobile banking etc. Our purpose is to trace various clustering techniques for malware detection.

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32

Jose, Shimi, and Radhika N. S. "Key Viruses of Pulses in Australia: General Detection and Management Methods." International Journal of Plant & Soil Science 37, no. 5 (2025): 389–98. https://doi.org/10.9734/ijpss/2025/v37i55462.

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Pulse crops, including chickpeas, lentils, faba beans, field peas etc., are vital to Australia’s agricultural economy, supporting domestic consumption, exports, and sustainable farming through nitrogen fixation. However, viral diseases pose a significant threat, causing substantial yield losses and compromising crop quality. This concise review synthesizes current knowledge on major viruses affecting Australian pulse crops, such as alfalfa mosaic virus (AMV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pea seed-borne mosaic virus (PSbMV), turnip mosaic virus (TuMV) and other

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Yuwen, Lihui, Shifeng Zhang, and Jie Chao. "Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection." Biosensors 13, no. 8 (2023): 822. http://dx.doi.org/10.3390/bios13080822.

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Virus-related infectious diseases are serious threats to humans, which makes virus detection of great importance. Traditional virus-detection methods usually suffer from low sensitivity and specificity, are time-consuming, have a high cost, etc. Recently, DNA biosensors based on DNA nanotechnology have shown great potential in virus detection. DNA nanotechnology, specifically DNA tiles and DNA aptamers, has achieved atomic precision in nanostructure construction. Exploiting the programmable nature of DNA nanostructures, researchers have developed DNA nanobiosensors that outperform traditional

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34

Toumasis, P., and G. Vrioni. "An overview of microbiological diagnostic methods in SARS-CoV-2 infection." ACTA MICROBIOLOGICA HELLENICA 66, no. 2-3 (2022): 121–32. https://doi.org/10.5281/zenodo.10077314.

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Since 2020, humanity is facing the pandemic of COVID-19 disease, with the scientific community looking for reliable ways to diagnose SARS-CoV-2 infection. The quiver of diagnostic methods includes three types of tests. In order to document acute SARS-CoV-2 virus infection, techniques for detecting the genetic material of the virus are used either by the reverse transcriptase polymerase chain reaction (rRT-PCR) method or by isothermal reaction techniques as well as antigens detection tests. Diagnostic tests aimed at detecting either specific binding or specific neutralizing antibodies to the SA

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35

Pan, Dequan, Wei Wang, and Tong Cheng. "Current Methods for the Detection of Antibodies of Varicella-Zoster Virus: A Review." Microorganisms 11, no. 2 (2023): 519. http://dx.doi.org/10.3390/microorganisms11020519.

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Infection with the varicella-zoster virus (VZV) causes chickenpox and shingles, which lead to significant morbidity and mortality globally. The detection of serum VZV-specific antibodies is important for the clinical diagnosis and sero-epidemiological research of VZV infection, and for assessing the effect of VZV vaccine immunization. Over recent decades, a variety of methods for VZV antibody detection have been developed. This review summarizes and compares the current methods for detecting VZV antibodies, and discussed future directions for this field.

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36

Petrović, Tamaš, Maja Velhner, Jelena Petrović, Igor Stojanov, Živoslav Grgić, and Sava Lazić. "MODERN LABORATORY DIAGNOSTIC METHODS IN VETERINARY MEDICINE AND THE POSSIBILITY OF ITS APPLICATION." Archives of Veterinary Medicine 3, no. 1 (2010): 39–61. http://dx.doi.org/10.46784/e-avm.v3i1.192.

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One of the greatest challenges of modern laboratory diagnosis is the selection of methods for fast, highly sensitive and specific detection of the infective agents. This challenge is present also in molecular diagnosing of causative agents in veterinary medicine. An example of molecular detection, the application of molecular diagnostic methods and procedures in the epizootiology of some viral infections is presented in the paper. The molecular methods play an important role in virus detection and surveillance. Out of a large number of molecular methods most frequently used are classical gel-b

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37

Halstead, D. C., S. Todd, and G. Fritch. "Evaluation of five methods for respiratory syncytial virus detection." Journal of Clinical Microbiology 28, no. 5 (1990): 1021–25. http://dx.doi.org/10.1128/jcm.28.5.1021-1025.1990.

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38

Zimmerman, Richard K., Charles R. Rinaldo, Mary Patricia Nowalk, et al. "Detection of Influenza Virus Infection Using Two PCR Methods." Advances in Virology 2014 (2014): 1–3. http://dx.doi.org/10.1155/2014/274679.

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Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This s

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39

Cambra, M., D. Boscia, A. Myrta, et al. "Detection and characterization of Plum pox virus: serological methods." EPPO Bulletin 36, no. 2 (2006): 254–61. http://dx.doi.org/10.1111/j.1365-2338.2006.00983.x.

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40

Olmos, A., N. Capote, and T. Candresse. "Detection and characterization of Plum pox virus: molecular methods." EPPO Bulletin 36, no. 2 (2006): 262–66. http://dx.doi.org/10.1111/j.1365-2338.2006.00984.x.

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Dovas, C. I., E. Hatziloukas, R. Salomon, E. Barg, Y. Shiboleth, and N. I. Katis. "Comparison of Methods for Virus Detection in Allium spp." Journal of Phytopathology 149, no. 11-12 (2001): 731–37. http://dx.doi.org/10.1046/j.1439-0434.2001.00705.x.

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Quint, W. G., R. A. Heijtink, J. Schirm, W. H. Gerlich, and H. G. Niesters. "Reliability of methods for hepatitis B virus DNA detection." Journal of clinical microbiology 33, no. 1 (1995): 225–28. http://dx.doi.org/10.1128/jcm.33.1.225-228.1995.

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Bosch, Albert, Gloria Sánchez, Morteza Abbaszadegan, et al. "Analytical Methods for Virus Detection in Water and Food." Food Analytical Methods 4, no. 1 (2010): 4–12. http://dx.doi.org/10.1007/s12161-010-9161-5.

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张, 力玲. "Progress of Hepatitis B Virus Biomarkers and Detection Methods." Medical Diagnosis 03, no. 04 (2013): 57–63. http://dx.doi.org/10.12677/md.2013.34013.

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45

Komínek, P. "Selection of RNA isolation method for molecular detection of grapevine viruses." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 267–70. http://dx.doi.org/10.17221/10463-pps.

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Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using Concert<sup>TM</sup> reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using Concert<sup>TM</sup> reagent. If this RNA was used

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46

Zhang, Yu, Shuxin Qu, and Liming Xu. "Progress in the study of virus detection methods: The possibility of alternative methods to validate virus inactivation." Biotechnology and Bioengineering 116, no. 8 (2019): 2095–102. http://dx.doi.org/10.1002/bit.27003.

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47

Singh, Rudra P. "Development of the molecular methods for potato virus and viroid detection and prevention." Genome 42, no. 4 (1999): 592–604. http://dx.doi.org/10.1139/g99-047.

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Potato is the fourth most important food crop in the world and it forms the diet of a billion consumers in developing countries, where potato production is increasing rapidly. However, potato virus diseases in developing countries are one of the major causes of lower yields. Their control requires the development of appropriate virus-detection and seed-production technologies for the region. Recent progress in developing nucleic acid based virus detection methods are reviewed. Refinements of the protocols applicable to the laboratories located in seed producing areas are discussed. Nucleic aci

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48

Rowhani, Adib, Lenka Biardi, Geoffrey Routh, Steve D. Daubert, and Deborah A. Golino. "Development of a Sensitive Colorimetric-PCR Assay for Detection of Viruses in Woody Plants." Plant Disease 82, no. 8 (1998): 880–84. http://dx.doi.org/10.1094/pdis.1998.82.8.880.

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Diagnostic methods employing the polymerase chain reaction (PCR) provide the most sensitive means currently available for detecting viruses in woody plants. A new technique has been tested that does not rely on gel electrophoresis or molecular hybridization to detect virus-specific PCR products. This colorimetric method for detection of PCR products from woody plants was demonstrated to be at least as sensitive as gel analysis. When combined with immunocapture of virions from plant sap, colorimetric detection provides a means to apply PCR technology to a large number of samples. Here, we repor

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LEGGITT, PARIS R., and LEE-ANN JAYKUS. "Detection Methods for Human Enteric Viruses in Representative Foods†." Journal of Food Protection 63, no. 12 (2000): 1738–44. http://dx.doi.org/10.4315/0362-028x-63.12.1738.

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Although viral foodborne disease is a significant problem, foods are rarely tested for viral contamination, and when done, testing is limited to shellfish commodities. In this work, we report a method to extract and detect human enteric viruses from alternative food commodities using an elution-concentration approach followed by detection using reverse transcription-polymerase chain reaction (RT-PCR). Fifty-gram lettuce or hamburger samples were artificially inoculated with poliovirus type 1 (PV1), hepatitis A virus (HAV), or the Norwalk virus and processed by the sequential steps of homogeniz

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Dhamad, Ahmed E., and Muna A. Abdal Rhida. "COVID-19: molecular and serological detection methods." PeerJ 8 (October 7, 2020): e10180. http://dx.doi.org/10.7717/peerj.10180.

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Abstract:

Since COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was declared as a pandemic disease by the World Health Organization in early 2020, many countries, organizations and companies have tried to find the best way to diagnose the virus and contain its spreading. SARS-CoV-2 is a positive-sense single RNA (+ssRNA) coronavirus and mainly spreads through droplets, respiratory secretions, and direct contact. The early detection of the virus plays a central role in lowering COVID19 incidents and mortality rates. Thus, finding a simple, accurate, cheap and quick detec

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