Relevant bibliographies by topics / Virus induced gene silencing

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Virus induced gene silencing'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 July 2025

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Journal articles on the topic "Virus induced gene silencing"

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Brigneti, Gianinna, Ana M. Martín-Hernández, Hailing Jin, et al. "Virus-induced gene silencing inSolanumspecies." Plant Journal 39, no. 2 (2004): 264–72. http://dx.doi.org/10.1111/j.1365-313x.2004.02122.x.

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Unver, Turgay, and Hikmet Budak. "Virus-Induced Gene Silencing, a Post Transcriptional Gene Silencing Method." International Journal of Plant Genomics 2009 (June 15, 2009): 1–8. http://dx.doi.org/10.1155/2009/198680.

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Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies have been advanced and modified for VIGS approach. Virus-derived inoculations are performed on host plants using different methods such as agro-infiltration and in vitro transcriptions. VIGS has many advan
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Liu, Yule, Michael Schiff, and S. P. Dinesh-Kumar. "Virus-induced gene silencing in tomato." Plant Journal 31, no. 6 (2002): 777–86. http://dx.doi.org/10.1046/j.1365-313x.2002.01394.x.

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Lu, R. "Virus-induced gene silencing in plants." Methods 30, no. 4 (2003): 296–303. http://dx.doi.org/10.1016/s1046-2023(03)00037-9.

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Gammelgård, Elin, Maradumane Mohan, and Jari P. T. Valkonen. "Potyvirus-induced gene silencing: the dynamic process of systemic silencing and silencing suppression." Journal of General Virology 88, no. 8 (2007): 2337–46. http://dx.doi.org/10.1099/vir.0.82928-0.

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Potato virus A (PVA; genus Potyvirus) was used for virus-induced gene silencing in a model system that included transgenic Nicotiana benthamiana (line 16c) expressing the gfp transgene for green fluorescent protein (GFP) and chimeric PVA (PVA–GFP) carrying gfp in the P1-encoding region. Infection of the 16c plants with PVA–GFP in five experiments resulted in a reproducible pattern of systemic gfp transgene silencing, despite the presence of the strong silencing-suppressor protein, HC-Pro, produced by the virus. PVA–GFP was also targeted by silencing, and virus-specific short interfering RNA ac
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Li, Hui-Liang, Dong Guo, Ying Wang, Jia-Hong Zhu, Long Qu, and Shi-Qing Peng. "Tobacco rattle virus–induced gene silencing in Hevea brasiliensis." Bioscience, Biotechnology, and Biochemistry 85, no. 3 (2021): 562–67. http://dx.doi.org/10.1093/bbb/zbaa085.

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ABSTRACT Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS in rubber tree has not yet been reported. In this study, we described the efficient gene silencing in rubber tree by VIGS. The gene encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified in rubber tree genome. Small interfering RNAs from HbPDS and the silencing gene fragment were predicted and a length of 399 bp was selected to be tested. We showed that the tobacco rattle virus (TRV)-VIGS could induce effective HbPDS silenc
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Nakano, Masahito, and Takafumi Mukaihara. "Virus-induced gene silencing in Solanum torvum." Journal of General Plant Pathology 88, no. 1 (2021): 10–16. http://dx.doi.org/10.1007/s10327-021-01040-7.

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Shao, Y., H. L. Zhu, H. Q. Tian, et al. "Virus-induced gene silencing in plant species." Russian Journal of Plant Physiology 55, no. 2 (2008): 168–74. http://dx.doi.org/10.1134/s1021443708020027.

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Burch-Smith, Tessa M., Michael Schiff, Yule Liu, and S. P. Dinesh-Kumar. "Efficient Virus-Induced Gene Silencing in Arabidopsis." Plant Physiology 142, no. 1 (2006): 21–27. http://dx.doi.org/10.1104/pp.106.084624.

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Fu, Da-Qi, Ben-Zhong Zhu, Hong-Liang Zhu, Wei-Bo Jiang, and Yun-Bo Luo. "Virus-induced gene silencing in tomato fruit." Plant Journal 43, no. 2 (2005): 299–308. http://dx.doi.org/10.1111/j.1365-313x.2005.02441.x.

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Dissertations / Theses on the topic "Virus induced gene silencing"

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Payne, Richard. "Gene discovery in Catharanthus roseus using virus induced gene silencing." Thesis, University of East Anglia, 2015. https://ueaeprints.uea.ac.uk/59379/.

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This thesis presents the use of Virus Induced Gene Silencing (VIGS) for the discovery of enzymes and transporters involved in monoterpene indole alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents in the treatment of a variety of cancers, however the complete biosynthetic pathway for these metabolites remains to be elucidated. Additionally, this metabolic pathway is subcellulary compartmented with the key branch point enzyme, strictosidine synthase, localised to the plant vacuole. There is th
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George, Gavin M. (Gavin Mager). "Virus induced gene silencing for the study of starch metabolism." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4024.

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Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Virus Induced Gene Silencing (VIGS) was optimized to allow for the study of starch metabolism. The plastidial inorganic pyrophosphatase gene, for which a mutant has never been identified, was studied using VIGS and it was found to have a broad role in this subcellular compartment. The accumulation of inorganic pyrophosphate limited the production of starch, carotenoids, chlorophyll, and increased the plants susceptibility to drought stress. These effects highlight the importance of this enzyme in m
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Starkus, Laura. "Virus-induced gene silencing of putative Diuraphis noxia (Kurdjumov) resistance genes in wheat." Thesis, Manhattan, Kan. : Kansas State University, 2010. http://hdl.handle.net/2097/4193.

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Jaggard, Daniel Andrew William. "The structure and function of RPW8.1 and RPW8.2, powdery mildew disease resistance proteins from Arabidopsis thaliana (L.) Heyhn." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251898.

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Kawai, Takashi. "Virus-induced gene silencing in Prunus fruit and nut tree species by Apple latent spherical virus vector." Kyoto University, 2017. http://hdl.handle.net/2433/217995.

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Kyoto University (京都大学)<br>0048<br>新制・論文博士<br>博士(農学)<br>乙第13073号<br>論農博第2843号<br>新制||農||1046(附属図書館)<br>学位論文||H29||N5029(農学部図書室)<br>33224<br>京都大学大学院農学研究科農学専攻<br>(主査)教授 北島 宣, 教授 土井 元章, 教授 田尾 龍太郎<br>学位規則第4条第2項該当
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Peart, Jack Robert. "The use of virus-induced gene silencing to identify genes required for N-mediated resistance against tobacco mosaic virus." Thesis, University of East Anglia, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247101.

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Demircan, Turan. "Application Of Virus Induced Gene Silencing Of Brachypodium Distachyon, A Model Organism For Crops." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12610649/index.pdf.

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Grass family is most important family in plant kingdom due to intensive usage of crops in agriculture. To date, molecular biology researches on grass family have had limitations because of inappropriate characteristics of barley and wheat to conduct experiments on them. Brachypodium distachyon that belongs to grass family has recently emerged as a model organism for crops. It shares common characteristics for a model plant due to its small genome, small physical plant size, a short lifecycle, and less demanding growth requirements<br>as other model organisms<br>Arabidopsis thaliana, Oryza s
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Lu, Rui. "High throughput virus induced gene silencing for the analysis of disease resistance in plants." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.398556.

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Lee, Jack Alexander. "The use of virus induced gene silencing to investigate Septoria leaf blotch in wheat." Thesis, Durham University, 2016. http://etheses.dur.ac.uk/11465/.

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Septoria leaf blotch, caused by the fungal pathogen Zymoseptoria tritici, is one of the most damaging diseases of wheat (Triticum aestivum), a crop plant of significant worldwide importance. Using the system of Virus-Induced Gene Silencing, to create transient knockdowns of target genes, a novel wheat gene, TaR1, was identified as playing a key role in the host response to this pathogen. Silencing this gene leads to the earlier onset of disease symptoms, but reduced reproduction of the causal pathogen. Sequence analysis, confocal microscopy and protein-protein interaction assays were used to d
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Bozhanaj, Kreshnik. "The Effect Of Virus Induced Gene Silencing Of Fas Associated Factor1 In Blumeria Graminis Infected Barley." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12611139/index.pdf.

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Cereal loss due to fungal pathogens is an ongoing setback in agriculture. Elucidating plant&rsquo<br>s resistance and susceptibility mechanisms against these cereal killers, promises progress in agriculture. In the way of understanding barley resistance against fungus Blumeria Graminis we silenced FAS-Associated Factor 1 (FAF1) gene in its mRNA level with Virus Induced Gene Silencing (VIGS) technique. Previous research in our lab had shown an augmentation in mRNA levels of FAF1 gene in fungus infected wheat, suggesting a role of this gene in the resistance mechanism. We hypothesized that the a
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Books on the topic "Virus induced gene silencing"

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Becker, Annette, ed. Virus-Induced Gene Silencing. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-278-0.

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Courdavault, Vincent, and Sébastien Besseau, eds. Virus-Induced Gene Silencing in Plants. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0751-0.

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Hayes, Ian MacDonald. Virus-induced changes in host gene expression in infected cowpeas. University of East Anglia, 1990.

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Plows, David John. Natural and induced variation in the fusion glycoprotein gene of human respiratory syncytial virus subgroup A. typescript, 1994.

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Becker, Annette. Virus-Induced Gene Silencing: Methods and Protocols. Humana Press, 2016.

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Virus-Induced Gene Silencing: Methods and Protocols. Humana Press, 2013.

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Besseau, Sébastien, and Vincent Courdavault. Virus-Induced Gene Silencing in Plants: Methods and Protocols. Springer, 2021.

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Courdavault, Vincent, and Sebastien Besseau. Virus-Induced Gene Silencing in Plants: Methods and Protocols. Springer, 2020.

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Munroe, Donald Gordon. Expression and mutation of the p53 gene in friend virus-induced murine erythroleukemia. 1991.

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Rij, Ronald P. van. Antiviral RNAi: Concepts, Methods, and Applications. Humana Press, 2016.

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Book chapters on the topic "Virus induced gene silencing"

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Kant, Ravi, and Indranil Dasgupta. "Virus-Induced Gene Silencing." In Plant Viruses. CRC Press, 2018. http://dx.doi.org/10.1201/9781315162287-21.

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Palmer, Lira, and Sarah E. O’Connor. "Virus-Induced Gene Silencing in Nepeta." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0751-0_9.

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Kachroo, Aardra, and Said Ghabrial. "Virus-Induced Gene Silencing in Soybean." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-882-5_19.

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Jiang, Cai-Zhong, Jen-Chih Chen, and Michael Reid. "Virus-Induced Gene Silencing in Ornamental Plants." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-123-9_6.

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Todd, Andrea T., Enwu Liu, and Jonathan E. Page. "cDNA Libraries for Virus-Induced Gene Silencing." In Plant Epigenetics. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-646-7_16.

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Yan, Huijun, Zhao Zhang, Jean-Louis Magnard, Benoît Boachon, Sylvie Baudino, and Kaixue Tang. "Virus-Induced Gene Silencing in Rose Flowers." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0751-0_16.

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Jia, Haifeng, and Yuanyue Shen. "Virus-Induced Gene Silencing in Strawberry Fruit." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-278-0_16.

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Bachan, Shawn, and Savithramma P. Dinesh-Kumar. "Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-882-5_6.

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Ricci, Angela, Silvia Sabbadini, Laura Miozzi, Bruno Mezzetti, and Emanuela Noris. "Host-induced gene silencing and spray-induced gene silencing for crop protection against viruses." In RNAi for plant improvement and protection. CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0008.

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Abstract Since the beginning of agriculture, plant virus diseases have been a strong challenge for farming. Following its discovery at the very beginning of the 1990s, the RNA interference (RNAi) mechanism has been widely studied and exploited as an integrative tool to obtain resistance to viruses in several plant species, with high target-sequence specificity. In this chapter, we describe and review the major aspects of host-induced gene silencing (HIGS), as one of the possible plant defence methods, using genetic engineering techniques. In particular, we focus our attention on the use of RNA
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Ricci, Angela, Silvia Sabbadini, Laura Miozzi, Bruno Mezzetti, and Emanuela Noris. "Host-induced gene silencing and spray-induced gene silencing for crop protection against viruses." In RNAi for plant improvement and protection. CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0072.

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Abstract Since the beginning of agriculture, plant virus diseases have been a strong challenge for farming. Following its discovery at the very beginning of the 1990s, the RNA interference (RNAi) mechanism has been widely studied and exploited as an integrative tool to obtain resistance to viruses in several plant species, with high target-sequence specificity. In this chapter, we describe and review the major aspects of host-induced gene silencing (HIGS), as one of the possible plant defence methods, using genetic engineering techniques. In particular, we focus our attention on the use of RNA
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Conference papers on the topic "Virus induced gene silencing"

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Fenwick, Ann, Rebecca L. Larson, Patrick A. Reeves, Christopher M. Richards, and Lee Panella. "Virus induced gene silencing of a gene repressing flowering in sugar beet." In American Society of Sugar Beet Technologist. ASSBT, 2007. http://dx.doi.org/10.5274/assbt.2007.30.

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Wintermantel, William M., and Laura L. Hladky. "Resistance to curly top viruses through virus induced gene silencing." In American Society of Sugarbeet Technologist. ASSBT, 2009. http://dx.doi.org/10.5274/assbt.2009.35.

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Болотина, А. А., А. В. Власова, А. В. Полховский та И. В. Киров. "ПРИМЕНЕНИЕ МЕТОДА VIGS С ИСПОЛЬЗОВАНИЕМ ВИРУСНЫХ СУПРЕССОРОВ ДЛЯ ПРЕОДОЛЕНИЯ РНК-ИНТЕРФЕРЕНЦИИ". У Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2023. http://dx.doi.org/10.48397/arriab.2023.23.xxiii.025.

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Достижения в технологии секвенирования следующего поколения привели к аккумулированию большого объема геномных данных, открыв новые перспективы для поиска функциональных генов. Для установления и изучения функций генов могут быть использованы методы обратной генетики, например с помощью сайленсинга их экспрессии. Вирус-индуцированнный сайленсинг генов (англ. Virus-Induced Gene Silencing, VIGS) – это метод, основанный на пост-транскрипционном подавлении генов (англ. - Post-Transcriptional Gene Silencing, PTGS), реализуемый с применением естественного механизма РНК-интерференции, используемого р
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Wintermantel, William M., Amy G. Anchieta, and Particia A. Nicely. "Development of novel sources of resistance to Beet curly top virus through virus-induced gene silencing." In American Society of Sugar Beet Technologist. ASSBT, 2007. http://dx.doi.org/10.5274/assbt.2007.35.

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Самарская, В. О., Н. А. Спеченкова, Н. В. Маркин, et al. "MECHANISMS INVOLVED IN THE PROTECTIVE ANTIVIRAL RESPONSE OF POTATO PLANTS DURING EXOGENOUS APPLICATION OF dsRNA AGAINST POTATO VIRUS Y." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2022. http://dx.doi.org/10.48397/arriab.2022.22.xxii.029.

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Технология опрыскивания растений экзогенной двуцепочечной РНК (дцРНК) или спрей-индуцированный сайленсинг генов применяется для индукции устойчивости растений к вирусам сельскохозяйственных культур [1]. В настоящей работе мы использовали этот подход для обработки растений картофеля Solanum tuberosum препаратами дцРНК, гомологичной консервативному фрагменту гена репликазы Y вируса картофеля (дцРНК-YВК), и изучили влияние экзогенной дцРНК на защитный ответ растений, основанный на механизмах специфической РНК интерференции (РНКи) [2] и неспецифического иммунитета, индуцируемого молекулярными патт
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Suprunova, T. P., N. V. Markin, A. N. Ignatov, A. G. Solovyov, N. O. Kalinina, and M. E. Talyansky. "Use of dsRNA-based antiviral compounds to protect potato plants." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-132.

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One of the most important food crops in the world, the potato (Solanum tuberosum L.) is infected with many viruses, of which the y virus (Potato virus Y, PVY) is the most important economically, causing significant crop losses. Several alternative methods of dsRNA delivery have been tested, with the most promising being spray - induced gene silencing (SIGS). The results showed a high effect of preventive use of dsRNA. Treatment with the initial working concentration of dsRNA protected 100% and 65% of plants from virus propagation for 14 and 21 days, respectively, and 65% of plants were protect
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"Enhancing plant defense: carbon dots for efficient spray-induced gene silencing." In Open-GPB. International Viticulture and Enology Society, 2024. http://dx.doi.org/10.58233/meanowhz.

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Kamarauli, E. D., A. V. Vlasova, D. V. Perevozchikov, and I. V. Kirov. "ACTIVATION MOBILE ELEMENTS TRANSCRIPTION AND TRANSPOSITION USING NOVEL APPROACH OF VIRUS-INDUCED TE-STORM." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-188.

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In this work, we represent a new TE-storm method for activation plant mobile elements. Our approach is based on transient suppression of the transposon silencing mechanisms using recombinant viral constructs. The development of TE-storm opens new possibilities for introducing mutations into the genome using the natural biological mutagens — mobile elements
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Polkhovskiy, A. V., M. V. Dmitrieva, and I. V. Kirov. "VIRUS-INDUCED GENE EDITING AS NEW BRANCH OF BIOLOGICAL MUTAGENESIS." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2024. https://doi.org/10.48397/v5286-2658-9360-t.

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Biological mutagenesis (BM) implies usage of natural mutagens, like transposable elements, to widen genetic variability of modern crops [1]. Virus-Induced Gene Editing (VIGE) is becoming yet another branch of BM.
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Schulman, ES, SC Pugliese, S. Ansaloni, et al. "RNA Interference-Induced Gene Silencing of Histidine Decarboxylase Produces Human Mast Cells Deficient in Histamine." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a3708.

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Reports on the topic "Virus induced gene silencing"

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Mawassi, Munir, Baozhong Meng, and Lorne Stobbs. Development of Virus Induced Gene Silencing Tools for Functional Genomics in Grapevine. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7613887.bard.

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Grapevine is perhaps the most widely grown fruit crop. To understand the genetic make-up so as to improve the yield and quality of grapes and grape products, researchers in Europe have recently sequenced the genomes of Pinot noir and its inbred. As expected, function of many grape genes is unknown. Functional genomics studies have become the major focus of grape researchers and breeders. Current genetic approaches for gene function studies include mutagenesis, crossing and genetic transformation. However, these approaches are difficult to apply to grapes and takes long periods of time to accom
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Aly, Radi, and John I. Yoder. Development of resistant crop plants to parasitic weeds based on trans-specific gene silencing. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7598146.bard.

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Broomrapes (Orobanche/Phelipanchespp.) are holo parasitic plants that subsist on the roots of a variety of agricultural crops and cause severe losses to the yield quality and quantity. Effective methods for controlling parasitic weeds are scarce, with only a few known cases of genetic resistance. In the current study, we proposed an improved strategy for the control of parasitic weeds based on trans-specific gene-silencing of three parasite genes at once. We used two strategies to express dsRNA containing selected sequences of three Phelipancheaegyptiacagenes PaACS, PaM6PR and PaPrx1 (pma): tr
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Gal-On, Amit, Shou-Wei Ding, Victor P. Gaba, and Harry S. Paris. role of RNA-dependent RNA polymerase 1 in plant virus defense. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597919.bard.

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Objectives: Our BARD proposal on the impact of RNA-dependent RNA polymerase 1 (RDR1) in plant defense against viruses was divided into four original objectives. 1. To examine whether a high level of dsRNA expression can stimulate RDR1 transcription independent of salicylic acid (SA) concentration. 2. To determine whether the high or low level of RDR1 transcript accumulation observed in virus resistant and susceptible cultivars is associated with viral resistance and susceptibility. 3. To define the biogenesis and function of RDR1-dependent endogenous siRNAs. 4. To understand why Cucumber mosai
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Whitham, Steven A., Amit Gal-On, and Tzahi Arazi. Functional analysis of virus and host components that mediate potyvirus-induced diseases. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7591732.bard.

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The mechanisms underlying the development of symptoms in response to virus infection remain to be discovered in plants. Insight into symptoms induced by potyviruses comes from evidence implicating the potyviral HC-Pro protein in symptom development. In particular, recent studies link the development of symptoms in infected plants to HC-Pro's ability to interfere with small RNA metabolism and function in plant hosts. Moreover, mutation of the highly conserved FRNK amino acid motif to FINK in the HC-Pro of Zucchini yellow mosaic virus (ZYMV) converts a severe strain into an asymptomatic strain,
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Mawassi, Munir, Adib Rowhani, Deborah A. Golino, Avichai Perl, and Edna Tanne. Rugose Wood Disease of Grapevine, Etiology and Virus Resistance in Transgenic Vines. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586477.bard.

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Rugose wood is a complex disease of grapevines, which occurs in all growing areas. The disease is spread in the field by vector transmission (mealybugs). At least five elongated-phloem- limited viruses are implicated in the various rugose wood disorders. The most fully characterized of these are Grapevine virus A (GV A) and GVB, members of a newly established genus, the vitivirus. GVC, a putative vitivirus, is much less well characterized than GV A or GVB. The information regarding the role of GVC in the etiology and epidemiology of rugose wood is fragmentary and no sequence data for GVC are a
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Meir, Shimon, Michael S. Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Senescence. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7592657.bard.

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Original objectives: To understand the regulation of abscission by exploring the nature of changes of auxin-related gene expression in tomato (Lycopersicon esculatumMill) abscission zones (AZs) following organ removal, and by analyzing the function of these genes. Our specific goals were: 1) To complete the microarray analyses in tomato flower and leaf AZs, for identifying genes whose expression changes early in response to auxin depletion; 2) To examine, using virus-induced gene silencing (VIGS), the effect of silencing target genes on ethylene sensitivity and abscission competence of the lea
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Wang, X. F., and M. Schuldiner. Systems biology approaches to dissect virus-host interactions to develop crops with broad-spectrum virus resistance. United States-Israel Binational Agricultural Research and Development Fund, 2020. http://dx.doi.org/10.32747/2020.8134163.bard.

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More than 60% of plant viruses are positive-strand RNA viruses that cause billion-dollar losses annually and pose a major threat to stable agricultural production, including cucumber mosaic virus (CMV) that infects numerous vegetables and ornamental trees. A highly conserved feature among these viruses is that they form viral replication complexes (VRCs) to multiply their genomes by hijacking host proteins and remodeling host intracellular membranes. As a conserved and indispensable process, VRC assembly also represents an excellent target for the development of antiviral strategies that can b
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Tucker, Mark L., Shimon Meir, Amnon Lers, Sonia Philosoph-Hadas, and Cai-Zhong Jiang. Elucidation of signaling pathways that regulate ethylene-induced leaf and flower abscission of agriculturally important plants. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597929.bard.

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The Problem: Abscission is a highly regulated process, occurring as a natural terminal stage of development, in which various organs are separated from the parent plant. In most plant species, the process is initiated by a decrease in active auxin in the abscission zone (AZ) and an increase in ethylene, and may be accelerated by postharvest or environmental stresses. Another potential key regulator in abscission is IDA (Inflorescence Deficient in Abscission), which was identified as an essential peptide signal for floral organ abscission in Arabidopsis. However, information is still lacking re
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Avni, Adi, and Kirankumar S. Mysore. Functional Genomics Approach to Identify Signaling Components Involved in Defense Responses Induced by the Ethylene Inducing Xyalanase Elicitor. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7697100.bard.

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Plant-microbe interactions involve a large number of global regulatory systems, which are essential for plants to protect themselves against pathogen attack. An ethylene-inducing xylanase (EIX) of Trichoderma viride is a potent elicitor of plant defense responses, like hypersensitive response (HR), in specific cultivars of tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum). The central goal of this proposal was to investigate the molecular mechanisms that allow plants to specifically activate defense responses after EIX treatment. We proposed to identify cellular signaling compon
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Meir, Shimon, Michael Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Abscission. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696523.bard.

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Original objectives: Understanding the regulation of abscission competence by exploring the nature and function of auxin-related gene expression changes in the leaf and pedicelAZs of tomato (as a model system), was the main goal of the previously submitted proposal. We proposed to achieve this goal by using microarray GeneChip analysis, to identify potential target genes for functional analysis by virus-induced gene silencing (VIGS). To increase the potential of accomplishing the objectives of the previously submitted proposal, we were asked by BARD to show feasibility for the use of these two
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