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1

Payne, Richard. "Gene discovery in Catharanthus roseus using virus induced gene silencing." Thesis, University of East Anglia, 2015. https://ueaeprints.uea.ac.uk/59379/.

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This thesis presents the use of Virus Induced Gene Silencing (VIGS) for the discovery of enzymes and transporters involved in monoterpene indole alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents in the treatment of a variety of cancers, however the complete biosynthetic pathway for these metabolites remains to be elucidated. Additionally, this metabolic pathway is subcellulary compartmented with the key branch point enzyme, strictosidine synthase, localised to the plant vacuole. There is th
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2

George, Gavin M. (Gavin Mager). "Virus induced gene silencing for the study of starch metabolism." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4024.

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Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Virus Induced Gene Silencing (VIGS) was optimized to allow for the study of starch metabolism. The plastidial inorganic pyrophosphatase gene, for which a mutant has never been identified, was studied using VIGS and it was found to have a broad role in this subcellular compartment. The accumulation of inorganic pyrophosphate limited the production of starch, carotenoids, chlorophyll, and increased the plants susceptibility to drought stress. These effects highlight the importance of this enzyme in m
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3

Starkus, Laura. "Virus-induced gene silencing of putative Diuraphis noxia (Kurdjumov) resistance genes in wheat." Thesis, Manhattan, Kan. : Kansas State University, 2010. http://hdl.handle.net/2097/4193.

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4

Jaggard, Daniel Andrew William. "The structure and function of RPW8.1 and RPW8.2, powdery mildew disease resistance proteins from Arabidopsis thaliana (L.) Heyhn." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251898.

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5

Kawai, Takashi. "Virus-induced gene silencing in Prunus fruit and nut tree species by Apple latent spherical virus vector." Kyoto University, 2017. http://hdl.handle.net/2433/217995.

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Kyoto University (京都大学)<br>0048<br>新制・論文博士<br>博士(農学)<br>乙第13073号<br>論農博第2843号<br>新制||農||1046(附属図書館)<br>学位論文||H29||N5029(農学部図書室)<br>33224<br>京都大学大学院農学研究科農学専攻<br>(主査)教授 北島 宣, 教授 土井 元章, 教授 田尾 龍太郎<br>学位規則第4条第2項該当
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6

Peart, Jack Robert. "The use of virus-induced gene silencing to identify genes required for N-mediated resistance against tobacco mosaic virus." Thesis, University of East Anglia, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247101.

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7

Demircan, Turan. "Application Of Virus Induced Gene Silencing Of Brachypodium Distachyon, A Model Organism For Crops." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12610649/index.pdf.

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Grass family is most important family in plant kingdom due to intensive usage of crops in agriculture. To date, molecular biology researches on grass family have had limitations because of inappropriate characteristics of barley and wheat to conduct experiments on them. Brachypodium distachyon that belongs to grass family has recently emerged as a model organism for crops. It shares common characteristics for a model plant due to its small genome, small physical plant size, a short lifecycle, and less demanding growth requirements<br>as other model organisms<br>Arabidopsis thaliana, Oryza s
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8

Lu, Rui. "High throughput virus induced gene silencing for the analysis of disease resistance in plants." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.398556.

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9

Lee, Jack Alexander. "The use of virus induced gene silencing to investigate Septoria leaf blotch in wheat." Thesis, Durham University, 2016. http://etheses.dur.ac.uk/11465/.

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Septoria leaf blotch, caused by the fungal pathogen Zymoseptoria tritici, is one of the most damaging diseases of wheat (Triticum aestivum), a crop plant of significant worldwide importance. Using the system of Virus-Induced Gene Silencing, to create transient knockdowns of target genes, a novel wheat gene, TaR1, was identified as playing a key role in the host response to this pathogen. Silencing this gene leads to the earlier onset of disease symptoms, but reduced reproduction of the causal pathogen. Sequence analysis, confocal microscopy and protein-protein interaction assays were used to d
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10

Bozhanaj, Kreshnik. "The Effect Of Virus Induced Gene Silencing Of Fas Associated Factor1 In Blumeria Graminis Infected Barley." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12611139/index.pdf.

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Cereal loss due to fungal pathogens is an ongoing setback in agriculture. Elucidating plant&rsquo<br>s resistance and susceptibility mechanisms against these cereal killers, promises progress in agriculture. In the way of understanding barley resistance against fungus Blumeria Graminis we silenced FAS-Associated Factor 1 (FAF1) gene in its mRNA level with Virus Induced Gene Silencing (VIGS) technique. Previous research in our lab had shown an augmentation in mRNA levels of FAF1 gene in fungus infected wheat, suggesting a role of this gene in the resistance mechanism. We hypothesized that the a
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11

Kim, Joonseog. "EFFECTS OF SILENCING CYC2-LIKE GENES ON FLORAL DEVELOPMENT IN SOLANUM LYCOPERSICUM L. AND NICOTIANA OBTUSIFOLIA M. MARTENS & GALEOTTI (SOLANACEAE)." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/5014.

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CYCLOIDEA (CYC) and DICHOTOMA (DICH) of the CYC2 clade of the TCP gene family have been shown to play a significant role in regulating the identity of the dorsal petals and abortion of the single dorsal stamen in Antirrhinum majus. It is believed that CYC2-like genes are responsible for the convergent evolution of floral zygomorphy, but their role in the development of actinomorphic flowers is still unknown. In Solanaceae, previous analysis has identified two paralogs of CYC2-like genes, CYC2A and CYC2B, resulting from a gene duplication that predates the origin the family. Virus-induced gene
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12

Lim, Gaik Wee Heidi Clinical School St Vincent's Hospital Faculty of Medicine UNSW. "Promoter targeted siRNAs induce transcriptional gene silencing (TGS) in Simian Immunodeficiency Virus (SIV)." Awarded By:University of New South Wales. Clinical School - St Vincent's Hospital, 2009. http://handle.unsw.edu.au/1959.4/44889.

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RNA interference is a phenomenon by which double-stranded RNA is processed into small interfering RNAs (siRNAs) that can cause gene silencing in plants, yeast, Drosophila (fruit fly) and human cells. Small interfering double stranded RNAs (siRNAs) can induce gene silencing via two pathways: post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS). PTGS involves siRNA triggering of sequence- specific degradation of mRNA takes place in the cell's cytoplasm. In contrast, siRNAs, which induce TGS have sequence complementary to regions within a gene's promoter and the act
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13

Mohammad, Hamza [Verfasser]. "Construction of a virus-induced gene silencing system based on Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV) / Hamza Mohammad." Hannover : Technische Informationsbibliothek (TIB), 2018. http://d-nb.info/1152965557/34.

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14

Du, Preez Jacques. "The development and characterisation of grapevine virus-based expression vectors." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4003.

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Thesis (PhD (Genetics))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Grapevine (Vitis vinifera L.) is a very important agricultural commodity that needs to be protected. To achieve this several in vivo tools are needed for the study of this crop and the pathogens that infect it. Recently the grapevine genome has been sequenced and the next important step will be gene annotation and function using these in vivo tools. In this study the use of Grapevine virus A (GVA), genus Vitivirus, family Flexiviridae, as transient expression and VIGS vector for heterologous protein expression
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15

Dagdas, Yasin Fatih. "Investigating The Roles Of Micrornas In Biotic Stress Responses And Functional Characterization Of A Novel Ztl-type F-box Protein Via Virus Induced Gene Silencing." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12610641/index.pdf.

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Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefore, molecular plant-pathogen interactions studies are starting to become the new tool to
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16

Sabbadini, Silvia. "Strategies for the genetic transformation, via organogenesis, of Prunus SPP to induce resistance to Plum Pox Virus (PPV) through post-transcriptional gene silencing." Doctoral thesis, Università Politecnica delle Marche, 2013. http://hdl.handle.net/11566/242763.

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Le drupacee, in par-colare il pesco (Prunus persica), sono tra le specie arboree più importan- col-vate nel bacino del Mediterraneo, tu9avia infezioni virali determinate in par-colare da Plum Pox Virus (PPV), agente eziologico della Sharka, sono la causa di notevoli perdite agronomiche ed economiche. Al momento non ci sono mezzi di lo9a dire9a contro tale infezione ma solo mezzi di prevenzione non sempre efficaci e con problemi di sostenibilità ambientale e di cos- per gli agricoltori. Per questo mo-vo mol- programmi di studi genomici e di breeding tradizionale sono finalizza- a conoscer
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17

Bunwaree, Heemee Devi. "Implementation of a genetic screen for the identification of resistances to beet virus yellows." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAJ042.

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Depuis l'interdiction des néonicotinoïdes dans l'Union Européenne, la production de betteraves sucrières est fortement menacée par des épidémies de jaunisses virales. Ces maladies sont causées par un complexe de plusieurs virus transmis par les pucerons. Parmi eux, les polérovirus, responsables de jaunisses modérées, tels que le beet mild yellowing virus (BMYV) et le beet chlorosis virus (BChV), sont particulièrement répandus. Afin d’améliorer le criblage des variétés de betteraves sucrières résistantes ou tolérantes aux jaunisses virales, nous avons mis au point un virus recombinant provoquan
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18

Ozturk, Ibrahim Kutay. "Elucidation Of The Role Of Gcn2 Gene In Response To Powdery Mildew Infection." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614563/index.pdf.

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Plant immune system is entirely based on the immunities of the individual cells in which systemic signals originate from the infection sites. Powdery mildew disease is one of the agents causing these infection sites, resulting in significant yield losses, if disease develops. Understanding the molecular basis of plant-pathogen interactions is the new trend for fighting against plant pathogens, since classical methods used in selection of resistant plants are becoming less and less efficient nowadays. Thus, finding out the genes which are responsible in plant&rsquo<br>s resistance is becoming v
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19

Krenz, Björn. "Gene Silencing und das Abutilon Mosaik Virus." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-32318.

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20

Thakare, Dhiraj, Jianwei Zhang, Rod A. Wing, Peter J. Cotty, and Monica A. Schmidt. "Aflatoxin-free transgenic maize using host-induced gene silencing." AMER ASSOC ADVANCEMENT SCIENCE, 2017. http://hdl.handle.net/10150/623199.

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Aflatoxins, toxic secondary metabolites produced by some Aspergillus species, are a universal agricultural economic problem and a critical health issue. Despite decades of control efforts, aflatoxin contamination is responsible for a global loss of millions of tons of crops each year. We show that host-induced gene silencing is an effective method for eliminating this toxin in transgenic maize. We transformed maize plants with a kernel-specific RNA interference (RNAi) gene cassette targeting the aflC gene, which encodes an enzyme in the Aspergillus aflatoxin biosynthetic pathway. After pathoge
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21

Hunter, B. C. "Genetic modifiers of hairpin-induced gene silencing in Arabidopsis thaliana." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604807.

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The Chalcone Synthase (<i>CHS</i>) gene codes for the first step of biosynthesis of the purple pigment anthocyanin. In this study, transgenic Arabidopsis plants with a hairpin construct homologous to part of the <i>CHS </i>coding sequence were used in a genetic screen to identify modifiers of hairpin silencing. Some putative genetic modifiers were identified, but these appear to be the result of transgene:transgene interaction based on shared promoter homology rather than being due to second site suppressors in the Arabidopsis genome. New transgenic Arabidopsis lines were made with hairpin con
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22

Wang, Huan. "Investigation of RNA-induced gene silencing in the fission yeast Schizosaccharomyces pombe." Thesis, Queen Mary, University of London, 2007. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1597.

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RNA interference (RNAi) is a gene regulating system to which can silence genes at both transcriptional and post-transcriptional levels. In this thesis, a ura4-based RNAi selective assay was developed in the fission yeast Schizosaccharomyces pombe: antisense & sense constructs of mutually inverted ura4 DNA fragments were inserted under the regulation of a thiamine-repressible promoter; when induced by omission of thiamine, these transcripts triggered S. pombe ura4 gene silencing at the molecular level with the efficiency of -30%. Although dozens of proteins involved in RNAi pathways has been id
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23

Choy, Yee-wai Elizabeth. "A study of Epstein-Barr virus-encoded small regulatory RNAs." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39557261.

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24

蔡依慧 and Yee-wai Elizabeth Choy. "A study of Epstein-Barr virus-encoded small regulatory RNAs." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39557261.

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25

au, J. Fosu@murdoch edu, and John Fosu-Nyarko. "Studies on Subterranean clover mottle virus towards development of a gene silencing vector." Murdoch University, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050421.123457.

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Subterranean clover mottle virus (SCMoV) is a positive sense, single-stranded RNA virus that infects subterranean clover (Trifolium subterraneum) and a number of related legume species. The ultimate aim of this research was to investigate aspects of SCMoV that would support its use as a gene silencing vector for legume species, since RNA (gene) silencing is now a potential tool for studylng gene function. The ability of viruses to induce an antiviral defense system is being explored by virus-induced gene silencing (VIGS), in which engmeered viral genomes are used as vectors to introduce genes
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Fosu-Nyarko, John. "Studies on Subterranean clover mottle virus towards development of a gene silencing vector." Thesis, Fosu-Nyarko, John (2005) Studies on Subterranean clover mottle virus towards development of a gene silencing vector. PhD thesis, Murdoch University, 2005. https://researchrepository.murdoch.edu.au/id/eprint/54/.

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Subterranean clover mottle virus (SCMoV) is a positive sense, single-stranded RNA virus that infects subterranean clover (Trifolium subterraneum) and a number of related legume species. The ultimate aim of this research was to investigate aspects of SCMoV that would support its use as a gene silencing vector for legume species, since RNA (gene) silencing is now a potential tool for studylng gene function. The ability of viruses to induce an antiviral defense system is being explored by virus-induced gene silencing (VIGS), in which engmeered viral genomes are used as vectors to introduce genes
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27

Fosu-Nyarko, John. "Studies on Subterranean clover mottle virus towards development of a gene silencing vector." Fosu-Nyarko, John (2005) Studies on Subterranean clover mottle virus towards development of a gene silencing vector. PhD thesis, Murdoch University, 2005. http://researchrepository.murdoch.edu.au/54/.

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Subterranean clover mottle virus (SCMoV) is a positive sense, single-stranded RNA virus that infects subterranean clover (Trifolium subterraneum) and a number of related legume species. The ultimate aim of this research was to investigate aspects of SCMoV that would support its use as a gene silencing vector for legume species, since RNA (gene) silencing is now a potential tool for studylng gene function. The ability of viruses to induce an antiviral defense system is being explored by virus-induced gene silencing (VIGS), in which engmeered viral genomes are used as vectors to introduce genes
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28

Lee, Hung-chiu. "Synthetic RNA interference against influenza A virus." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B35537814.

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29

Lee, Hung-chiu, and 李洪釗. "Synthetic RNA interference against influenza A virus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B35537814.

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30

Gammelgård, Elin. "Interactions of potato virus A with host plants : recombination, gene silencing and non-hypersensitive resistance /." Uppsala : Dept. of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/2007111.pdf.

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31

Wagner, Laura A. "Silencing mutant Huntingtin by RNA interference for the treatment of Huntington Disease." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/937.

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Huntington Disease (HD) is a dominantly inherited neurological disease attributed to a CAG expansion within the HD gene. The HD mutation gives rise to a polyglutamine expansion in exon 1 of the protein huntingtin (Htt). Since the discovery of the HD mutation in 1993, various HD gene mouse models have been developed to contain either fragments or full-length copies of the mutant HD gene. The existence of these HD mouse models enables focused therapeutic testing to develop potential treatments for HD. RNA interference (RNAi) therapy is a targeted gene silencing approach whereby synthetic RNA con
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32

Hayes, Ian MacDonald. "Virus-induced changes in host gene expression in infected cowpeas." Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.277172.

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33

Buchmann, Cody. "Reversal of RNA-mediated gene silencing pathways by geminivirus AL2 and L2 proteins." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1221847080.

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34

Van, Eeden C. (Christiaan). "The construction of gene silencing transformation vectors for the introduction of multiple-virus resistance in grapevines." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53764.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Viruses are some of the most important pathogens of grapevines. There are no effective chemical treatments, and no grapevine- or other natural resistance genes have been discovered against grapevine infecting viruses. The primary method of grapevine virus control is prevention by biological indexing and molecular- and serological screening of rootstocks and scions before propagation. Due to the spread of grapevine viruses through insect vectors, and in the case of GRSPaV the absence of serological screening, these metho
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Howard-Till, Rachel A. "Small RNA pathways and the roles of tudor nucleases in gene silencing and DNA deletion in Tetrahymena thermopila /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/5064.

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36

Dung, Le Phuong [Verfasser]. "Analysis of sense transgene-induced gene silencing in introgression lines reveals the presence of silencing modulators in Arabidopsis thaliana accession genomes / Le Phuong Dung." Halle, 2017. http://d-nb.info/1132840481/34.

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37

Deddouche, Safia. "Regulation and function of the virus induced gene Vago in drosophila." Strasbourg, 2009. http://www.theses.fr/2009STRA6263.

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Des infections virales continuent à être une cause majeure de morbidité et de mortalité dans le monde entier. En particulier, ces trois dernières décennies ont été témoin de l’apparition d'environ 25 nouvelles maladies virales. De plus, les récentes pandémies, comme celle associé au virus du Chikungunya à la Réunion, démontrent l'énorme problème de santé publique associé aux infections virales transmisses par les arthropodes. À cette étape, notre compréhension de la réponse du vecteur face à l'infection virale est très limitée. Le but de mes études de doctorat était d'utiliser la Drosophile me
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38

Hoffmann, Laurent. "Etude du métabolisme des phénylpropanoïdes; analyse de l'interaction de la caféoyl-coenzyme A 3-O-méthyltransférase (CCoAOMT) avec son substrat et caractérisation fonctionnelle d'une nouvelle acyltransférase, l'HydroxyCinnamoyl-CoA : shikimate/quinate hydroxycinnamoyl Transférase (HCT)." Phd thesis, Université Louis Pasteur - Strasbourg I, 2003. http://tel.archives-ouvertes.fr/tel-00003598.

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Le métabolisme des phénylpropanoïdes est un métabolisme secondaire spécifique au règne végétal. Il conduit, à partir de la phénylalanine, à la synthèse d'une grande variété de substances telles que les anthocyanes, les isoflavonoïdes, les stilbènes, des esters d'acides hydroxycinnamiques, ou encore à la lignine. Ces métabolites secondaires interviennent dans la pigmentation florale ou encore la protection des tissus végétaux contre divers stress biotiques et abiotiques. Quant à la lignine, elle assure rigidité aux parois cellulaires végétales et imperméabilité aux tissus conducteurs. La lignin
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Elmén, Joacim. "Nucleic acid based therapeutic approaches /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-047-8/.

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40

Pathi, Krishna [Verfasser]. "Establishment of maize resistance to fungal diseases by host-induced gene silencing and site-directed mutagenesis / Krishna Pathi." Hannover : Gottfried Wilhelm Leibniz Universität, 2021. http://d-nb.info/1235138437/34.

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41

Wong, Kam-wai, and 黃錦偉. "The molecular mechanism of mitotic arrest induced by a novel diterpenoid pseudolaric acid B and a novel gene encoding RNA-bindingprotein 22." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37679090.

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Wong, Kam-wai. "The molecular mechanism of mitotic arrest induced by a novel diterpenoid pseudolaric acid B and a novel gene encoding RNA-binding protein 22." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37679090.

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43

Chu, Chia-Ying. "Molecular Mechanism of RNA-Mediated Gene Silencing in Human Cells: A Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/388.

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Small non-coding RNAs regulate gene expression at posttranscriptional level in eukaryotic cells. Two classes of such small (~21-25 nt) RNAs that have been extensively studied in gene silencing are short interfering RNAs (siRNAs) and microRNAs (miRNAs). RNA interference (RNAi) is process whereby double-stranded RNA induces the sequence-specific degradation of homologous mRNA. The RNAi machinery can also be programmed in human cells by introducing 21-nt siRNA duplexes that are assembled into RNA-induced silencing complexes (RISC). In this dissertation, systematic analysis of siRNAs with deletion
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Nauth, Brittany J. "Soybean QTL Mapping and Candidate Gene Identification for Pythium irregulare and Phytophthora sojae Partial Resistance; and Root-Knot Nematode Induced Suppression of Gene Silencing." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406151869.

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Guan, Haoji, and 關浩基. "Double-stranded RNA induced gene silencing of neuropeptide genes in sand shrimp, Metapenaeus ensis and development of crustacean primarycell culture." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B36893626.

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Guan, Haoji. "Double-stranded RNA induced gene silencing of neuropeptide genes in sand shrimp, Metapenaeus ensis and development of crustacean primary cell culture /." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36893626.

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47

Rupp, Jessica Lynn Shoup. "RNA interference mediated virus resistance in transgenic wheat." Diss., Kansas State University, 2015. http://hdl.handle.net/2097/20387.

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Doctor of Philosophy<br>Plant Pathology<br>John P. Fellers<br>Harold N. Trick<br>Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are two viruses affecting wheat in the Great Plains region of the United States. Genetic resistance is severely limited, requiring management methods focusing on the deployment of resistant varieties and various cultural practices. Evaluation of resistance is complicated by the lack of a standard rating scale. The objective of this work was to develop new avenues to mitigate these challenges. A standardized virus symptom rating scale was developed
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48

Zhang, Chunquan. "GENETIC DIVERSITY OF BEAN POD MOTTLE VIRUS (BPMV) AND DEVELOPMENT OF BPMV AS A VECTOR FOR GENE EXPRESSION IN SOYBEAN." UKnowledge, 2005. http://uknowledge.uky.edu/gradschool_diss/437.

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Bean pod mottle virus (BPMV), a member of the genus Comovirus in the family Comoviridae, is widespread in the major soybean-growing areas in the United States. The complete nucleotide sequences of the genomic RNAs of the naturally occurring partial diploid strain IL-Cb1 were determined. Intermolecular RNA1 recombinants were isolated from strain IL-Cb1 and characterized at the molecular level. Structurally similar recombinant RNA1 was also generated after four passages in soybean derived from plants previously inoculated with a mixture of infectious RNA1 transcripts from two distinct strains. B
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49

Höfle, Lisa [Verfasser]. "Investigations on the mechanism of Host-Induced Gene Silencing of the fungal CYP51 genes in the Fusarium – Arabidopsis pathosystem / Lisa Höfle." Gießen : Universitätsbibliothek, 2018. http://d-nb.info/1163945307/34.

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50

Owor, Betty Elizabeth. "Maize streak virus (MSV) diversity in Uganda and the assessment of gene silencing as a tool for development of resistance to MSV." Doctoral thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/4315.

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Includes abstract.<br>Includes bibliographical references (leaves 158-197).<br>Maize streak virus (MSV: Family Geminiviridae, Genus Mastrevirus) is the causal agent of maize streak disease (MSD) that contributes significantly to low maize yields in Africa, thereby threatening food security of sub-Saharan Africa’s poorest people. In Uganda, MSD has been identified as one of the most important constraints to maize production. In order to have a better understanding of the disease in that country, this thesis set out to establish MSD levels in farmers’ fields; develop a new sampling and virus iso
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