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Journal articles on the topic 'Virus induced gene silencing'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Brigneti, Gianinna, Ana M. Martín-Hernández, Hailing Jin, et al. "Virus-induced gene silencing inSolanumspecies." Plant Journal 39, no. 2 (2004): 264–72. http://dx.doi.org/10.1111/j.1365-313x.2004.02122.x.

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2

Unver, Turgay, and Hikmet Budak. "Virus-Induced Gene Silencing, a Post Transcriptional Gene Silencing Method." International Journal of Plant Genomics 2009 (June 15, 2009): 1–8. http://dx.doi.org/10.1155/2009/198680.

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Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies have been advanced and modified for VIGS approach. Virus-derived inoculations are performed on host plants using different methods such as agro-infiltration and in vitro transcriptions. VIGS has many advan

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3

Liu, Yule, Michael Schiff, and S. P. Dinesh-Kumar. "Virus-induced gene silencing in tomato." Plant Journal 31, no. 6 (2002): 777–86. http://dx.doi.org/10.1046/j.1365-313x.2002.01394.x.

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4

Lu, R. "Virus-induced gene silencing in plants." Methods 30, no. 4 (2003): 296–303. http://dx.doi.org/10.1016/s1046-2023(03)00037-9.

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5

Gammelgård, Elin, Maradumane Mohan, and Jari P. T. Valkonen. "Potyvirus-induced gene silencing: the dynamic process of systemic silencing and silencing suppression." Journal of General Virology 88, no. 8 (2007): 2337–46. http://dx.doi.org/10.1099/vir.0.82928-0.

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Potato virus A (PVA; genus Potyvirus) was used for virus-induced gene silencing in a model system that included transgenic Nicotiana benthamiana (line 16c) expressing the gfp transgene for green fluorescent protein (GFP) and chimeric PVA (PVA–GFP) carrying gfp in the P1-encoding region. Infection of the 16c plants with PVA–GFP in five experiments resulted in a reproducible pattern of systemic gfp transgene silencing, despite the presence of the strong silencing-suppressor protein, HC-Pro, produced by the virus. PVA–GFP was also targeted by silencing, and virus-specific short interfering RNA ac

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6

Li, Hui-Liang, Dong Guo, Ying Wang, Jia-Hong Zhu, Long Qu, and Shi-Qing Peng. "Tobacco rattle virus–induced gene silencing in Hevea brasiliensis." Bioscience, Biotechnology, and Biochemistry 85, no. 3 (2021): 562–67. http://dx.doi.org/10.1093/bbb/zbaa085.

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ABSTRACT Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS in rubber tree has not yet been reported. In this study, we described the efficient gene silencing in rubber tree by VIGS. The gene encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified in rubber tree genome. Small interfering RNAs from HbPDS and the silencing gene fragment were predicted and a length of 399 bp was selected to be tested. We showed that the tobacco rattle virus (TRV)-VIGS could induce effective HbPDS silenc

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7

Nakano, Masahito, and Takafumi Mukaihara. "Virus-induced gene silencing in Solanum torvum." Journal of General Plant Pathology 88, no. 1 (2021): 10–16. http://dx.doi.org/10.1007/s10327-021-01040-7.

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8

Shao, Y., H. L. Zhu, H. Q. Tian, et al. "Virus-induced gene silencing in plant species." Russian Journal of Plant Physiology 55, no. 2 (2008): 168–74. http://dx.doi.org/10.1134/s1021443708020027.

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9

Burch-Smith, Tessa M., Michael Schiff, Yule Liu, and S. P. Dinesh-Kumar. "Efficient Virus-Induced Gene Silencing in Arabidopsis." Plant Physiology 142, no. 1 (2006): 21–27. http://dx.doi.org/10.1104/pp.106.084624.

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10

Fu, Da-Qi, Ben-Zhong Zhu, Hong-Liang Zhu, Wei-Bo Jiang, and Yun-Bo Luo. "Virus-induced gene silencing in tomato fruit." Plant Journal 43, no. 2 (2005): 299–308. http://dx.doi.org/10.1111/j.1365-313x.2005.02441.x.

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11

Corbin, Cyrielle, Florent Lafontaine, Liuda Johana Sepúlveda, et al. "Virus-induced gene silencing in Rauwolfia species." Protoplasma 254, no. 4 (2017): 1813–18. http://dx.doi.org/10.1007/s00709-017-1079-y.

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12

Martin, Ruth C., Kira Glover-Cutter, Robert R. Martin, and James E. Dombrowski. "Virus induced gene silencing in Lolium temulentum." Plant Cell, Tissue and Organ Culture (PCTOC) 113, no. 2 (2012): 163–71. http://dx.doi.org/10.1007/s11240-012-0257-z.

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13

Lentz, Ezequiel Matias, Joel-Elias Kuon, Adrian Alder, et al. "Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots." Plant Methods 14, no. 1 (2018): 73. https://doi.org/10.1186/s13007-018-0340-5.

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<strong>Aim: </strong>We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (<i>Manihot esculenta</i> Crantz) leaves and roots. The African cassava mosaic virus isolate from Nigeria (ACMV-[NOg]), which was initially cloned in a binary vector for agroinoculation assays, was modified for application as VIGS vector. The functionality of the VIGS vector was validated in <i>Nicotiana benthamiana</i> and subsequently applied in wild-type and transgenic cassava plants expressing the <i>uidA</i> gene under the control o

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14

Tian, Yue, Yao Fang, Kaixin Zhang, et al. "Applications of Virus-Induced Gene Silencing in Cotton." Plants 13, no. 2 (2024): 272. http://dx.doi.org/10.3390/plants13020272.

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Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become an effective tool to investigate gene function in plants. Cotton is one of the most important economic crops globally. In the past decade, VIGS has been successfully applied in cotton functional genomic studies, including those examining abiotic and biotic stress responses and vegetative and reproductive development. This article summarizes the traditional vectors used in the cotton VIGS system, the visible markers used for endogenous gene silencing, the applications of VIGS in cotton functional g

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15

Ruiz, M. Teresa, Olivier Voinnet, and David C. Baulcombe. "Initiation and Maintenance of Virus-Induced Gene Silencing." Plant Cell 10, no. 6 (1998): 937. http://dx.doi.org/10.2307/3870680.

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16

Liu, Haiping, Daqi Fu, Benzhong Zhu, et al. "Virus-induced Gene Silencing in Eggplant (Solanum melongena)." Journal of Integrative Plant Biology 54, no. 6 (2012): 422–29. http://dx.doi.org/10.1111/j.1744-7909.2012.01102.x.

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17

Meng, Lan-Huan, Rui-Heng Wang, Ben-Zhong Zhu, Hong-Liang Zhu, Yun-Bo Luo, and Da-Qi Fu. "Efficient Virus-Induced Gene Silencing in Solanum rostratum." PLOS ONE 11, no. 6 (2016): e0156228. http://dx.doi.org/10.1371/journal.pone.0156228.

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18

Ruiz, M. Teresa, Olivier Voinnet, and David C. Baulcombe. "Initiation and Maintenance of Virus-Induced Gene Silencing." Plant Cell 10, no. 6 (1998): 937–46. http://dx.doi.org/10.1105/tpc.10.6.937.

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19

Sung, Y. C., C. P. Lin, and J. C. Chen. "Optimization of virus-induced gene silencing inCatharanthus roseus." Plant Pathology 63, no. 5 (2014): 1159–67. http://dx.doi.org/10.1111/ppa.12186.

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20

Nishii, Kanae, Yue Fei, Andrew Hudson, Michael Möller, and Attila Molnar. "Virus-induced Gene Silencing in Streptocarpus rexii (Gesneriaceae)." Molecular Biotechnology 62, no. 6-7 (2020): 317–25. http://dx.doi.org/10.1007/s12033-020-00248-w.

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21

Zhang, Yao, Nazi Niu, Shijia Li, et al. "Virus-Induced Gene Silencing (VIGS) in Chinese Jujube." Plants 12, no. 11 (2023): 2115. http://dx.doi.org/10.3390/plants12112115.

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Virus-induced gene silencing (VIGS) is a fast and efficient method for assaying gene function in plants. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully practiced in some species such as cotton and tomato. However, little research of VIGS systems has been reported in woody plants, nor in Chinese jujube. In this study, the TRV-VIGS system of jujube was firstly investigated. The jujube seedlings were grown in a greenhouse with a 16 h light/8 h dark cycle at 23 °C. After the cotyledon was fully unfolded, Agrobacterium mixture containing pTRV1 and pTRV2-ZjC

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22

Wang, Zhiquan, Xiaoyang Xu, Longjie Ni, Jinbo Guo, and Chunsun Gu. "Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus." PeerJ 7 (August 12, 2019): e7505. http://dx.doi.org/10.7717/peerj.7505.

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Background Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. The genetic transformation H. hamabo is currently inefficient and laborious, restricting gene functional studies on this species. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods In this study, we tested the efficiency of a tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfilt

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23

Jaberolansar, N., J. Hayati, H. Rajabi-Memari, SA Hosseini-Tafreshi, and D. Nabati-Ahmadi. "Tomato and Tobacco Phytoene Desaturase Gene Silencing by Virus-Induced Gene Silencing (VIGS) Technique." Iranian Journal of Virology 4, no. 1 (2010): 7–11. http://dx.doi.org/10.21859/isv.4.1.7.

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24

Bruun-Rasmussen, Marianne, Christian Toft Madsen, Stine Jessing, and Merete Albrechtsen. "Stability of Barley stripe mosaic virus–Induced Gene Silencing in Barley." Molecular Plant-Microbe Interactions® 20, no. 11 (2007): 1323–31. http://dx.doi.org/10.1094/mpmi-20-11-1323.

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Virus-induced gene silencing (VIGS) can be used as a powerful tool for functional genomics studies in plants. With this approach, it is possible to target most genes and downregulate the messenger (m)RNA in a sequence-specific manner. Barley stripe mosaic virus (BSMV) is an established VIGS vector for barley and wheat; however, silencing using this vector is generally transient, with efficient silencing often being confined to the first two or three systemically infected leaves. To investigate this further, part of the barley Phytoene desaturase (PDS) gene was inserted into BSMV and the result

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25

Hiriart, Jean-Baptiste, Eva-Mari Aro, and Kirsi Lehto. "Dynamics of the VIGS-Mediated Chimeric Silencing of the Nicotiana benthamiana ChlH Gene and of the Tobacco Mosaic Virus Vector." Molecular Plant-Microbe Interactions® 16, no. 2 (2003): 99–106. http://dx.doi.org/10.1094/mpmi.2003.16.2.99.

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The ChlH gene, encoding for the H subunit of the magnesium chelatase enzyme, was silenced in Nicotiana bentahamiana plants by virus-induced gene silencing (VIGS), using tobacco mosaic virus (TMV) expression vector. Strong silencing of the ChlH target gene was initiated only in the apical tissues, in which the endogenous transcription level of the target gene and the level of TMV vector RNA were both very high. The virus vector was also targeted by VIGS, and its suppression was correlated with the silencing of the ChlH mRNA. In the apical tissues, the suppression of both the virus vector and th

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26

Zulfiqar, Sumer, Muhammad Awais Farooq, Tiantian Zhao, et al. "Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics." International Journal of Molecular Sciences 24, no. 6 (2023): 5608. http://dx.doi.org/10.3390/ijms24065608.

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Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that has evolved into an indispensable approach for analyzing the function of genes. It downregulates endogenous genes by utilizing the posttranscriptional gene silencing (PTGS) machinery of plants to prevent systemic viral infections. Based on recent advances, VIGS can now be used as a high-throughput tool that induces heritable epigenetic modifications in plants through the viral genome by transiently knocking down targeted gene expression. As a result of the progression of DNA methylation induced by VIGS, new

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27

Wu, Zetang, Yali Zhu, David M. Bisaro, and Deborah S. Parris. "Herpes Simplex Virus Type 1 Suppresses RNA-Induced Gene Silencing in Mammalian Cells." Journal of Virology 83, no. 13 (2009): 6652–63. http://dx.doi.org/10.1128/jvi.00260-09.

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ABSTRACT RNA-induced silencing is a potent innate antiviral defense strategy in plants, and suppression of silencing is a hallmark of pathogenic plant viruses. However, the impact of silencing as a mammalian antiviral defense mechanism and the ability of mammalian viruses to suppress silencing in natural host cells have remained controversial. The ability of herpes simplex virus type 1 (HSV-1) to suppress silencing was examined in a transient expression system that employed an imperfect hairpin to target degradation of transcripts encoding enhanced green fluorescent protein (EGFP). HSV-1 infec

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28

Romero, Irene, Yury Tikunov, and Arnaud Bovy. "Virus-induced gene silencing in detached tomatoes and biochemical effects of phytoene desaturase gene silencing." Journal of Plant Physiology 168, no. 10 (2011): 1129–35. http://dx.doi.org/10.1016/j.jplph.2010.12.020.

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29

Cheng, Guanghao, Xiaochun Shu, Zhong Wang, Ning Wang, and Fengjiao Zhang. "Establishing a Virus-Induced Gene Silencing System in Lycoris chinensis." Plants 12, no. 13 (2023): 2458. http://dx.doi.org/10.3390/plants12132458.

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Lycoris is an important plant with both medicinal and ornamental values. However, it does not have an efficient genetic transformation system, which makes it difficult to study gene function of the genus. Virus-induced gene silencing (VIGS) is an effective technique for studying gene functions in plants. In this study, we develop an efficient virus-induced gene-silencing (VIGS) system using the leaf tip needle injection method. The widely used TRV vector is constructed, and the Cloroplastos Alterados 1 (CLA1) and Phytoene Desaturase (PDS) genes are selected as visual indicators in the VIGS sys

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30

Zeng, Lingen, Hui Zhang, Jinju Guo, et al. "Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd." Horticulturae 10, no. 2 (2024): 110. http://dx.doi.org/10.3390/horticulturae10020110.

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The aim of this study is to facilitate the construction of virus-induced gene silencing vectors and to provide a reference or positive control for gene silencing in bitter gourd. A recombinant TRSV (tobacco ringspot virus) containing two components, pTRSV1 and pTRSV2, was used in this study. The fragment of the McPDS target was cloned into pTRSV2 via combined enzymic ligation during digestion. The TRSV components were agro-infiltrated into tobacco leaves to grow virus particles, which were then extracted and mechanically inoculated into the bitter gourd plants. The effect of TRSV-McPDS-mediate

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31

Senthil-Kumar, Muthappa, and Kirankumar S. Mysore. "Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana." Nature Protocols 9, no. 7 (2014): 1549–62. http://dx.doi.org/10.1038/nprot.2014.092.

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32

Chong, Xinran, Yue Wang, Xiaoyang Xu, et al. "Efficient Virus-Induced Gene Silencing in Ilex dabieshanensis Using Tobacco Rattle Virus." Forests 14, no. 3 (2023): 488. http://dx.doi.org/10.3390/f14030488.

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Ilex dabieshanensis is not only an important ornamental plant, but can also be used to produce Kuding tea, owing to its lipid-lowering and anti-inflammatory medicinal properties. The genetic transformation of I. dabieshanensis is currently difficult, which restricts functional gene studies and molecular breeding research on this species. Virus-induced gene silencing (VIGS) is a powerful tool for determining gene functions in plants. The present study reports the first application of VIGS mediated by a tobacco rattle virus (TRV) vector in I. dabieshanensis. We tested the efficiency of the VIGS

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33

Zhu, Feng, Yanping Che, Fei Xu, et al. "Simultaneous silencing of two target genes using virus-induced gene silencing technology in Nicotiana benthamiana." Zeitschrift für Naturforschung C 74, no. 5-6 (2019): 151–59. http://dx.doi.org/10.1515/znc-2018-0071.

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Abstract Virus-induced gene silencing (VIGS) is an effective strategy for rapid gene function analysis. It is well established that the NAC transcription factor and salicylic acid (SA) signal pathway play essential roles in response to biotic stresses. However, simultaneous silencing of two target genes using VIGS in plants has been rarely reported. Therefore, in this report, we performed VIGS to silence simultaneously the SA-binding protein 2 (NbSABP2) and NbNAC1 in Nicotiana benthamiana to investigate the gene silencing efficiency of simultaneous silencing of two genes. We first cloned the f

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34

Burton, Rachel A., David M. Gibeaut, Antony Bacic, et al. "Virus-Induced Silencing of a Plant Cellulose Synthase Gene." Plant Cell 12, no. 5 (2000): 691. http://dx.doi.org/10.2307/3870995.

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35

Liu, Na, Ke Xie, Qi Jia, et al. "Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants." Plant Physiology 171, no. 3 (2016): 1801–7. http://dx.doi.org/10.1104/pp.16.00010.

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36

Burton, Rachel A., David M. Gibeaut, Antony Bacic, et al. "Virus-Induced Silencing of a Plant Cellulose Synthase Gene." Plant Cell 12, no. 5 (2000): 691–705. http://dx.doi.org/10.1105/tpc.12.5.691.

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37

Scofield, Steven R., and Richard S. Nelson. "Resources for Virus-Induced Gene Silencing in the Grasses." Plant Physiology 149, no. 1 (2009): 152–57. http://dx.doi.org/10.1104/pp.108.128702.

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38

Vaistij, Fabián E., and Louise Jones. "Compromised Virus-Induced Gene Silencing in RDR6-Deficient Plants." Plant Physiology 149, no. 3 (2009): 1399–407. http://dx.doi.org/10.1104/pp.108.132688.

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39

Baulcombe, David C. "Fast forward genetics based on virus-induced gene silencing." Current Opinion in Plant Biology 2, no. 2 (1999): 109–13. http://dx.doi.org/10.1016/s1369-5266(99)80022-3.

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40

van Kammen, A. "Virus-induced gene silencing in infected and transgenic plants." Trends in Plant Science 2, no. 11 (1997): 409–11. http://dx.doi.org/10.1016/s1360-1385(97)01128-x.

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41

Ido, Yukari, Kenji S. Nakahara, and Ichiro Uyeda. "White clover mosaic virus-induced gene silencing in pea." Journal of General Plant Pathology 78, no. 2 (2012): 127–32. http://dx.doi.org/10.1007/s10327-012-0360-3.

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42

Hongzhi, Wang, Ma Rongcai, Li Ruifen, Wang Guoying, and Wei Jianhua. "Virus-induced silencing of a tobacco deoxyhypusine synthase gene." Chinese Science Bulletin 50, no. 23 (2005): 2707–13. http://dx.doi.org/10.1007/bf02899640.

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43

Wang, Changchun, Xinzhong Cai, Xuemin Wang, and Zhong Zheng. "Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis." Functional Plant Biology 33, no. 4 (2006): 347. http://dx.doi.org/10.1071/fp05096.

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Arabidopsis thaliana (L.) Heynh. is a model plant species in which to study plant gene functions. Recently developed virus-induced gene silencing (VIGS) offers a rapid and high-throughput technique platform for gene function analysis. In this paper we report optimisation of tobacco rattle virus (TRV)-induced gene silencing in Arabidopsis. The parameters potentially affecting the efficiency of VIGS in Arabidopsis were investigated. These included the concentration and pre-incubation of Agrobacterium inocula (agro-inocula), the concentration of acetosyringone included in agro-inocula, the Agroba

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44

Villanueva-Alonzo, Hernán de Jesús, Ana Paulina Haro-Álvarez, Arturo A. Alvarado-Segura, et al. "A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity." Applied Sciences 12, no. 11 (2022): 5329. http://dx.doi.org/10.3390/app12115329.

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Plants have evolved defense mechanisms to suppress viral transcription and replication by transcriptional and post-transcriptional gene silencing mediated by virus-derived small interfering RNAs (vsiRNAs). Based on this response, virus-induced gene silencing (VIGS)-based technology has been developed to silence target genes on either host plants or insect pests. This mechanism could also be used for the silencing of genes of interest in the medical field. We used the VIGS vector pEuMV-YP:Krt18, which was obtained by inserting the Mus musculus (M. musculus) Krt18 sequence into pEuMV-YP:ΔAV1. Th

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45

Zeng, Hongqiu, Yanwei Xie, Guoyin Liu, Yunxie Wei, Wei Hu, and Haitao Shi. "Agrobacterium-Mediated Gene Transient Overexpression and Tobacco Rattle Virus (TRV)-Based Gene Silencing in Cassava." International Journal of Molecular Sciences 20, no. 16 (2019): 3976. http://dx.doi.org/10.3390/ijms20163976.

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Agrobacterium-mediated transient expression and virus-induced gene silencing (VIGS) are very useful in functional genomics in plants. However, whether these methods are effective in cassava (Manihot esculenta), one of the most important tropical crops, remains elusive. In this study, we used green fluorescent protein (GFP) and β-glucuronidase (GUS) as reporter genes in a transient expression assay. GFP or GUS could be detected in the infiltrated leaves at 2 days postinfiltration (dpi) and were evidenced by visual GFP and GUS assays, reverse-transcription PCR, and Western blot. In addition, phy

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46

Lu, Hsiang-Chia, Ming-Hsien Hsieh, Cheng-En Chen, Hong-Hwa Chen, Hsiang-Iu Wang, and Hsin-Hung Yeh. "A High-Throughput Virus-Induced Gene-Silencing Vector for Screening Transcription Factors in Virus-Induced Plant Defense Response in Orchid." Molecular Plant-Microbe Interactions® 25, no. 6 (2012): 738–46. http://dx.doi.org/10.1094/mpmi-10-11-0266.

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The large number of species and worldwide spread of species of Orchidaceae indicates their successful adaptation to environmental stresses. Thus, orchids provide rich resources to study how plants have evolved to cope with stresses. This report describes our improvement of our previously reported orchid virus-induced gene silencing vector, pCymMV-pro60, with a modified Gateway cloning system which requires only one recombination and can be inoculated by agroinfiltration. We cloned 1,700 DNA fragments, including 187 predicted transcription factors derived from an established expression sequence

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47

Tahereh, Borna. "Using Heterologous Virus-Induced Gene Silencing in Ipomoea purpurea as a Sister Family of Solanceae." Journal of Plant Biochemistry & Physiology 11, no. 1 (2023): 5. https://doi.org/10.35248/2329-9029.23.11.271.

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This study was conducted to investigate the possibility of using existing vectors as an heterologous system to silence genes in other plant species. In this study I measured the effectiveness of pre-existing Virus Induced Gene Silencing (VIGS) vector as a tool for gene silencing in Ipomoea purpurea, Ocimum basilicum and Anethum graveolens. The results showed that TRV-based VIGS vector with heterologous Phytoene Desaturase (PDS) sequences from Nicotiana bentamiana silenced the endogenous PDS gene in Ipomoea purpurea but not in Ocimum basilicum and Anethum graveolens.

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48

Ko, Na Yeon, Hyoun Sub Lim, Yong Man Yu, and Young Nam Youn. "Construction of cDNA Library for Using Virus-induced Gene Silencing (VIGS) Vector with the Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)." Korean Journal of Applied Entomology 54, no. 2 (2015): 91–97. http://dx.doi.org/10.5656/ksae.2015.04.0.004.

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49

Meng, Chunying, Jun Chen, Jinrong Peng, and Sek-Man Wong. "Host-induced avirulence of hibiscus chlorotic ringspot virus mutants correlates with reduced gene-silencing suppression activity." Journal of General Virology 87, no. 2 (2006): 451–59. http://dx.doi.org/10.1099/vir.0.81578-0.

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Post-transcriptional gene silencing (PTGS) and virus-encoded gene-silencing suppressors are defence and counterdefence strategies developed by host and pathogens during evolution. Using a green fluorescence protein-based transient suppression system, the coat protein (CP) of Hibiscus chlorotic ringspot virus (HCRSV) was identified as a strong gene-silencing suppressor. CP suppressed sense RNA-induced but not dsRNA-induced local and systemic PTGS. This is different from another virus in the genus Carmovirus, Turnip crinkle virus (TCV), the CP of which strongly suppresses dsRNA-induced PTGS. HCR

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Deng, Xianbao, Jani Kelloniemi, Tuuli Haikonen, et al. "Modification of Tobacco rattle virus RNA1 to Serve as a VIGS Vector Reveals That the 29K Movement Protein Is an RNA Silencing Suppressor of the Virus." Molecular Plant-Microbe Interactions® 26, no. 5 (2013): 503–14. http://dx.doi.org/10.1094/mpmi-12-12-0280-r.

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Abstract:

Tobacco rattle virus (TRV) has a bipartite, positive-sense single-stranded RNA genome and is widely used for virus-induced gene silencing (VIGS) in plants. RNA1 of TRV that lacks the gene for the cysteine-rich 16K silencing-suppression protein infects plants systemically in the absence of RNA2. Here, we attempted to engineer RNA1 for use as a VIGS vector by inserting heterologous gene fragments to replace 16K. The RNA1 vector systemically silenced the phytoene desaturase (PDS) gene, although less efficiently than when the original VIGS vector system was used, which consists of wild-type RNA1 a

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