Relevant bibliographies by topics / Virus-induced genome editing

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Academic literature on the topic 'Virus-induced genome editing'

Author: Grafiati
Published: 5 June 2025
Last updated: 8 July 2025

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Journal articles on the topic "Virus-induced genome editing"

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Oh, Youngbin, Hyeonjin Kim, and Sang-Gyu Kim. "Virus-induced plant genome editing." Current Opinion in Plant Biology 60 (April 2021): 101992. http://dx.doi.org/10.1016/j.pbi.2020.101992.

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Zhang, Chao, Shanhe Liu, Xuan Li, Ruixuan Zhang, and Jun Li. "Virus-Induced Gene Editing and Its Applications in Plants." International Journal of Molecular Sciences 23, no. 18 (2022): 10202. http://dx.doi.org/10.3390/ijms231810202.

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CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of plant genomes, have revolutionized plant science and enabled the creation of germplasms with beneficial traits. In order to apply these technologies, CRISPR/Cas reagents must be delivered into plant cells; however, this is limited by tissue culture challenges. Recently, viral vectors have been used to deliver CRISPR/Cas reagents into plant cells. Virus-induced genome editing (VIGE) has emerged as a powerful method with several advantages, including high editing efficiency and a simplified process for generating gene-edited DNA-free plants. Here, we briefly describe CRISPR/Cas-based genome editing. We then focus on VIGE systems and the types of viruses used currently for CRISPR/Cas9 cassette delivery and genome editing. We also highlight recent applications of and advances in VIGE in plants. Finally, we discuss the challenges and potential for VIGE in plants.
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Uranga, Mireia, Marta Vazquez-Vilar, Diego Orzáez, and José-Antonio Darós. "CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors." CRISPR Journal 4, no. 5 (2021): 761–69. https://doi.org/10.1089/crispr.2021.0049.

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The use of viral vectors that can replicate and move systemically through the host plant to deliver bacterialCRISPR components enables genome editing at the whole-plant level and avoids the requirement for labor-intensive stable transformation. However, this approach usually relies on previously transformed plants thatstably express a CRISPR-Cas nuclease. Here, we describe successful DNA-free genome editing ofNicotiana ben-thamianausing two compatible RNA virus vectors derived from tobacco etch virus (TEV; genusPotyvirus) andpotato virus X (PVX; genusPotexvirus), which replicate in the same cells. The TEV and PVX vectors respectivelyexpress a Cas12a nuclease and the corresponding guide RNA. This novel two-virus vector system improvesthe toolbox for transformation-free virus-induced genome editing in plants and will advance efforts to breedmore nutritious, resistant, and productive crops.
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Uranga, Mireia, Verónica Aragonés, Sara Selma, Marta Vázquez-Vilar, Diego Orzáez, and José-Antonio Darós. "Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector." Plant Journal 106, no. 2 (2021): 555–65. https://doi.org/10.1111/tpj.15164.

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Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas strategies in plants rely on genetic transformation using&nbsp;<em>Agrobacterium tumefaciens</em>&nbsp;to supply the gene editing reagents, such as Cas nucleases or the synthetic guide RNA (sgRNA). While Cas nucleases are constant elements in editing approaches, sgRNAs are target-specific and a screening process is usually required to identify those most effective. Plant virus-derived vectors are an alternative for the fast and efficient delivery of sgRNAs into adult plants, due to the virus capacity for genome amplification and systemic movement, a strategy known as virus-induced genome editing. We engineered&nbsp;<em>Potato virus X</em>&nbsp;(PVX) to build a vector that easily expresses multiple sgRNAs in adult solanaceous plants. Using the PVX-based vector,&nbsp;<em>Nicotiana</em>&nbsp;<em>benthamiana</em>&nbsp;genes were efficiently targeted, producing nearly 80% indels in a transformed line that constitutively expresses&nbsp;<em>Streptococcus pyogenes</em>&nbsp;Cas9. Interestingly, results showed that the PVX vector allows expression of arrays of unspaced sgRNAs, achieving highly efficient multiplex editing in a few days in adult plant tissues. Moreover, virus-free edited progeny can be obtained from plants regenerated from infected tissues or infected plant seeds, which exhibit a high rate of heritable biallelic mutations. In conclusion, this new PVX vector allows easy, fast and efficient expression of sgRNA arrays for multiplex CRISPR-Cas genome editing and will be a useful tool for functional gene analysis and precision breeding across diverse plant species, particularly in&nbsp;<em>Solanaceae</em>&nbsp;crops.
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Mikhaylova, Elena. "Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution." International Journal of Molecular Sciences 26, no. 10 (2025): 4599. https://doi.org/10.3390/ijms26104599.

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There is currently a worldwide trend towards deregulating the use of genome-edited plants. Virus-induced genome editing (VIGE) is a novel technique that utilizes viral vectors to transiently deliver clustered regularly interspaced short palindromic repeat (CRISPR) components into plant cells. It potentially allows us to obtain transgene-free events in any plant species in a single generation without in vitro tissue culture. This technology has great potential for agriculture and is already being applied to more than 14 plant species using more than 20 viruses. The main limitations of VIGE include insufficient vector capacity, unstable expression of CRISPR-associated (Cas) protein, plant immune reaction, host specificity, and reduced viral activity in meristem. Various solutions to these problems have been proposed, such as fusion of mobile elements, RNAi suppressors, novel miniature Cas proteins, and seed-borne viruses, but the final goal has not yet been achieved. In this review, the mechanism underlying the ability of different classes of plant viruses to transiently edit genomes is explained. It not only focuses on the latest achievements in virus-induced editing of crops but also provides suggestions for improving the technology. This review may serve as a source of new ideas for those planning to develop new approaches in VIGE.
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Ghoshal, Basudev, Brandon Vong, Colette L. Picard, Suhua Feng, Janet M. Tam, and Steven E. Jacobsen. "A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana." PLOS Genetics 16, no. 12 (2020): e1008983. http://dx.doi.org/10.1371/journal.pgen.1008983.

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Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication and movement. Utilizing this concept, several viral-based guide RNA delivery platforms for CRISPR-Cas9 genome editing have been developed. The CRISPR-Cas9 system has also been adapted for epigenome editing. While systems have been developed for CRISPR-Cas9 based gene activation or site-specific DNA demethylation, viral delivery of guide RNAs remains to be developed for these purposes. To address this gap we have developed a tobacco rattle virus (TRV)-based single guide RNA delivery system for epigenome editing in Arabidopsis thaliana. Because tRNA-like sequences have been shown to facilitate the cell-to-cell movement of RNAs in plants, we used the tRNA-guide RNA expression system to express guide RNAs from the viral genome to promote heritable epigenome editing. We demonstrate that the tRNA-gRNA system with TRV can be used for both transcriptional activation and targeted DNA demethylation of the FLOWERING WAGENINGEN gene in Arabidopsis. We achieved up to ~8% heritability of the induced demethylation phenotype in the progeny of virus inoculated plants. We did not detect the virus in the next generation, indicating effective clearance of the virus from plant tissues. Thus, TRV delivery, combined with a specific tRNA-gRNA architecture, provides for fast and effective epigenome editing.
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Fondong, Vincent N., Ugrappa Nagalakshmi, and Savithramma P. Dinesh-Kumar. "Novel Functional Genomics Approaches: A Promising Future in the Combat Against Plant Viruses." Phytopathology® 106, no. 10 (2016): 1231–39. http://dx.doi.org/10.1094/phyto-03-16-0145-fi.

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Advances in functional genomics and genome editing approaches have provided new opportunities and potential to accelerate plant virus control efforts through modification of host and viral genomes in a precise and predictable manner. Here, we discuss application of RNA-based technologies, including artificial micro RNA, transacting small interfering RNA, and Cas9 (clustered regularly interspaced short palindromic repeat–associated protein 9), which are currently being successfully deployed in generating virus-resistant plants. We further discuss the reverse genetics approach, targeting induced local lesions in genomes (TILLING) and its variant, known as EcoTILLING, that are used in the identification of plant virus recessive resistance gene alleles. In addition to describing specific applications of these technologies in plant virus control, this review discusses their advantages and limitations.
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Surya Krishna, Sakthivel, S. R. Harish Chandar, Maruthachalam Ravi, et al. "Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges." Agronomy 13, no. 4 (2023): 1000. http://dx.doi.org/10.3390/agronomy13041000.

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Sugarcane (Saccharum spp.) is one of the most valuable food and industrial crops. Its production is constrained due to major biotic (fungi, bacteria, viruses and insect pests) and abiotic (drought, salt, cold/heat, water logging and heavy metals) stresses. The ever-increasing demand for sugar and biofuel and the rise of new pest and disease variants call for the use of innovative technologies to speed up the sugarcane genetic improvement process. Developing new cultivars through conventional breeding techniques requires much time and resources. The advent of CRISPR/Cas genome editing technology enables the creation of new cultivars with improved resistance/tolerance to various biotic and abiotic stresses. The presence of genome editing cassette inside the genome of genome-edited plants hinders commercial exploitation due to regulatory issues. However, this limitation can be overcome by using transgene-free genome editing techniques. Transgene-free genome editing approaches, such as delivery of the RNPs through biolistics or protoplast fusion, virus-induced genome editing (VIGE), transient expression of CRISPR/Cas reagents through Agrobacterium-mediated transformation and other approaches, are discussed. A well-established PCR-based assay and advanced screening systems such as visual marker system and Transgene killer CRISPR system (TKC) rapidly identify transgene-free genome edits. These advancements in CRISPR/Cas technology speed up the creation of genome-edited climate-smart cultivars that combat various biotic and abiotic stresses and produce good yields under ever-changing conditions.
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Akhmetollayeva, A. S., and Sh A. Manabayeva. "CREATION OF AN EXPRESSION VECTOR FOR MULTIPLEX EDITING OF THE POTATO VACUOLAR INVERTASE GENE USING THE CRISPR/CAS9 SYSTEM." Eurasian Journal of Applied Biotechnology, no. 3 (October 3, 2024): 43–55. http://dx.doi.org/10.11134/btp.3.2024.5.

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Creating genetic engineering constructs for plant genome editing requires considerable time, material resources, and specialized equipment. A thorough understanding of the functions and efficacy of each construct element is critical to the design of specialized vectors for specific tasks. This article provides a detailed overview of the process of creating genetic engineering constructs for editing the invertase gene responsible for cold-induced sweetening (CIS) in potato tubers, starting with the design of the variable part of the guide RNA and ending with the assembly of the final expression vector for potato cell transformation. For this purpose, an analysis of the nucleotide sequences of the invertase gene from domestic potato varieties was performed, along with a comparative analysis the data from the NCBI database. Optimal targets for gene editing using CRISPR/Cas9 technology were identified and the process of cloning two expression vectors for multiple genome editing was described. The expression vectors obtained allow the knockout of the potato acid invertase gene VInv/Pain-1. Considering that the expression level of Cas9 is a key factor for the efficiency of genome editing, a second expression vector containing the Tomato bushy stunt virus suppressor gene p19 was created to enhance the expression of this gene and the editing efficiency.
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Matoušková, Magda, Jiří Plachý, Dana Kučerová, et al. "Rapid adaptive evolution of avian leukosis virus subgroup J in response to biotechnologically induced host resistance." PLOS Pathogens 20, no. 8 (2024): e1012468. http://dx.doi.org/10.1371/journal.ppat.1012468.

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Genetic editing of the germline using CRISPR/Cas9 technology has made it possible to alter livestock traits, including the creation of resistance to viral diseases. However, virus adaptability could present a major obstacle in this effort. Recently, chickens resistant to avian leukosis virus subgroup J (ALV-J) were developed by deleting a single amino acid, W38, within the ALV-J receptor NHE1 using CRISPR/Cas9 genome editing. This resistance was confirmed both in vitro and in vivo. In vitro resistance of W38-/- chicken embryonic fibroblasts to all tested ALV-J strains was shown. To investigate the capacity of ALV-J for further adaptation, we used a retrovirus reporter-based assay to select adapted ALV-J variants. We assumed that adaptive mutations overcoming the cellular resistance would occur within the envelope protein. In accordance with this assumption, we isolated and sequenced numerous adapted virus variants and found within their envelope genes eight independent single nucleotide substitutions. To confirm the adaptive capacity of these substitutions, we introduced them into the original retrovirus reporter. All eight variants replicated effectively in W38-/- chicken embryonic fibroblasts in vitro while in vivo, W38-/- chickens were sensitive to tumor induction by two of the variants. Importantly, receptor alleles with more extensive modifications have remained resistant to the virus. These results demonstrate an important strategy in livestock genome engineering towards antivirus resistance and illustrate that cellular resistance induced by minor receptor modifications can be overcome by adapted virus variants. We conclude that more complex editing will be necessary to attain robust resistance.
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Book chapters on the topic "Virus-induced genome editing"

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Uranga, Mireia. "Virus-Induced Genome Editing." In CRISPR and Plant Functional Genomics. CRC Press, 2024. http://dx.doi.org/10.1201/9781003387060-5.

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Kim, Hyeonjin, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim. "Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuata." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2879-9_16.

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A. Luke, Garry, and Martin D. Ryan. "The 2A Story: The End of the Beginning." In Beyond the Blueprint - Decoding the Elegance of Gene Expression [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1004928.

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Translational control of viral gene expression is a fundamental process essential for the vitality of all viruses. In special cases, signals encoded in the mRNA reprogram the ribosome to read the message in a different way, a process termed “translational recoding”. The 2A region of the foot-and-mouth disease virus (FMDV) encodes a short sequence, only 18 amino acids, that mediates self-processing by a novel translational effect “ribosome skipping” rather than proteolysis. Briefly, 2A interacts with the ribosome exit tunnel to inhibit peptide bond formation at the C terminus of the 2A sequence. Translation terminates at this point, but then resumes elongation, creating a second independent protein product. Thus, discrete proteins can be produced from a single transcript. The 2A sequence is particularly useful in vector strategies (AAV and retroviral vectors) where the capacity to incorporate foreign DNA is limited. Use of 2A and “2A-like” peptides to link the sequences encoding several proteins in the same open reading frame has led to their increasing use as important tools in biotechnology and biomedicine. This technology has been crucial for the visual tracking of expressed proteins, human gene therapies targeting cancer, production of induced human pluripotent stem cells for regenerative medicine, creation of transgenic animals and plants and the improvement of CRISPR-Cas9 and TALEN genome editing methods.
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Conference papers on the topic "Virus-induced genome editing"

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Polkhovskiy, A. V., M. V. Dmitrieva, and I. V. Kirov. "VIRUS-INDUCED GENE EDITING AS NEW BRANCH OF BIOLOGICAL MUTAGENESIS." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2024. https://doi.org/10.48397/v5286-2658-9360-t.

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Biological mutagenesis (BM) implies usage of natural mutagens, like transposable elements, to widen genetic variability of modern crops [1]. Virus-Induced Gene Editing (VIGE) is becoming yet another branch of BM.
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