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Journal articles on the topic 'Virus viability'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Aydogdu, Mehmet Onur, Esra Altun, Etelka Chung, et al. "Surface interactions and viability of coronaviruses." Journal of The Royal Society Interface 18, no. 174 (2021): 20200798. http://dx.doi.org/10.1098/rsif.2020.0798.

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The recently emerged coronavirus pandemic (COVID-19) has become a worldwide threat affecting millions of people, causing respiratory system related problems that can end up with extremely serious consequences. As the infection rate rises significantly and this is followed by a dramatic increase in mortality, the whole world is struggling to accommodate change and is trying to adapt to new conditions. While a significant amount of effort is focused on developing a vaccine in order to make a game-changing anti-COVID-19 breakthrough, novel coronavirus (SARS-CoV-2) is also developing mutations rap

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2

Püschell, K., F. Mohsenian, R. Laufs, S. Polywka, and M. Ermer. "Postmortem viability of the human immunodeficiency virus." International Journal of Legal Medicine 104, no. 2 (1991): 109–10. http://dx.doi.org/10.1007/bf01626041.

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3

Shervani, Zameer, Intazam Khan, Noha Yamin Siddiqui, Tooba Khan, and Umair Yaqub Qazi. "Viability of SARS-CoV-2 and Sanitization Methods." European Journal of Medical and Health Sciences 3, no. 1 (2021): 22–27. http://dx.doi.org/10.24018/ejmed.2021.3.1.665.

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Aerosol transmitted COVID-19 virus (SARS-CoV-2) is infectious causing both symptomatic and asymptomatic infections. The viable COVID-19 virus transmission via air has occurred. Therefore, methods must be established to determine the viability of the virus and to disinfect it in air, surfaces, and on foodstuff. In this review article, we have discussed the pattern virus spread in bus, hospital, and cruise ship. The methods of reducing and eliminating the infection in household and public places have been suggested. The safety, efficacy, and phase 3 trials data published by major vaccine candida

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4

Tran, Yen-Nhi, Jeffrey Kemp, and Paul Nash. "Preservation of Raboral V-RG® viability by vitrification (VAC9P.1100)." Journal of Immunology 194, no. 1_Supplement (2015): 145.8. http://dx.doi.org/10.4049/jimmunol.194.supp.145.8.

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Abstract Raboral V-RG® is a recombinant vaccinia virus used to orally immunize wildlife such as raccoons and coyotes against rabies. Since it is a live virus vaccine that expresses the rabies virus glycoprotein, stabilizing the virus and allowing for more methods of incorporation into bait can both be advantageous. This may also be true for other live viruses or cells. Two methods of preserving the virus were tested: vitrification and centrifugal evaporation. Vitrification in a sugar or polymer matrix provides protection for the virus through the transition of the matrix into a glass state and

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Chen, Huosheng, Laurie Ngo, Svetlana Petrovskaya, Yamei Gao, Majid Laassri, and Steven Rubin. "Purification of mumps virus particles of high viability." Journal of Virological Methods 233 (July 2016): 6–9. http://dx.doi.org/10.1016/j.jviromet.2016.03.003.

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6

Sun, Lichang, Yanhua Li, Runxia Liu, et al. "Porcine reproductive and respiratory syndrome virus ORF5a protein is essential for virus viability." Virus Research 171, no. 1 (2013): 178–85. http://dx.doi.org/10.1016/j.virusres.2012.11.005.

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7

Kroschewski, Helga, Jose-Luis Sagripanti, and Andrew D. Davidson. "Identification of amino acids in the dengue virus type 2 envelope glycoprotein critical to virus infectivity." Journal of General Virology 90, no. 10 (2009): 2457–61. http://dx.doi.org/10.1099/vir.0.011486-0.

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The dengue virus envelope glycoprotein mediates virus attachment and entry and is the major viral antigen. The identification of ‘critical’ amino acids in the envelope glycoprotein that cannot be altered without loss of infectivity could have a major impact on the development of dengue virus vaccines and diagnostics. In this context, we determined whether six amino acids, previously predicted by computational analysis to play a critical role in the virus life cycle, were essential for virus viability. The effects of mutating the six ‘critical’ amino acids and a further seven ‘neutral’ amino ac

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Homza, Miroslav, Hana Zelena, Jaroslav Janosek, et al. "Five Antigen Tests for SARS-CoV-2: Virus Viability Matters." Viruses 13, no. 4 (2021): 684. http://dx.doi.org/10.3390/v13040684.

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Antigen testing for SARS-CoV-2 (AGT) is generally considered inferior to RT-PCR testing in terms of sensitivity. However, little is known about the infectiousness of RT-PCR positive patients who pass undetected by AGT. In a screening setting for mildly symptomatic or asymptomatic patients with high COVID-19 prevalence (30–40%), 1141 patients were tested using one of five AGTs and RT-PCR. Where the results differed, virus viability in the samples was tested on cell culture (CV-1 cells). The test battery included AGTs by JOYSBIO, Assure Tech, SD Biosensor, VivaChek Biotech and NDFOS. Sensitiviti

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Lin, Kaisen, Chase R. Schulte та Linsey C. Marr. "Survival of MS2 and Φ6 viruses in droplets as a function of relative humidity, pH, and salt, protein, and surfactant concentrations". PLOS ONE 15, № 12 (2020): e0243505. http://dx.doi.org/10.1371/journal.pone.0243505.

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The survival of viruses in droplets is known to depend on droplets’ chemical composition, which may vary in respiratory fluid between individuals and over the course of disease. This relationship is also important for understanding the persistence of viruses in droplets generated from wastewater, freshwater, and seawater. We investigated the effects of salt (0, 1, and 35 g/L), protein (0, 100, and 1000 μg/mL), surfactant (0, 1, and 10 μg/mL), and droplet pH (4.0, 7.0, and 10.0) on the viability of viruses in 1-μL droplets pipetted onto polystyrene surfaces and exposed to 20%, 50%, and 80% rela

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Nuradji, Harimurti, Rahmat Setya Adji, and Qadrina Ayu Besticia. "The effect of heat and disinfectants on the viability of infectious bursal disease virus." BIO Web of Conferences 33 (2021): 06008. http://dx.doi.org/10.1051/bioconf/20213306008.

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Infectious Bursal Disease (IBD) has been reported in Indonesia since 1983 and has become an endemic disease. IBD virus is known to be quite resistant to physical and chemical reagents compared to other viruses, causing this disease is hard to be eradicated. This study aims to evaluate the effect of heat and disinfectants on the viability of the IBD virus. This study was conducted using a local isolate of IBDV obtained from IRCVS. The virus was exposed to heat and disinfectant. Heat treatment was conducted by exposing the virus to 560C and 600C for 30, 60, 120, and 300 minutes. Similarly, the v

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11

Mustafa, Zulkifli, Hilda Shazana Shamsuddin, Aini Ideris, et al. "Viability Reduction andRac1Gene Downregulation of HeterogeneousEx-VivoGlioma Acute Slice Infected by the Oncolytic Newcastle Disease Virus Strain V4UPM." BioMed Research International 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/248507.

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Oncolytic viruses have been extensively evaluated for anticancer therapy because this virus preferentially infects cancer cells without interfering with normal cells. Newcastle Disease Virus (NDV) is an avian virus and one of the intensively studied oncolytic viruses affecting many types of cancer including glioma. Nevertheless, the capability of NDV infection on heterogeneous glioma tissue in a cerebrospinal fluid atmosphere has never been reported. Recently,Rac1is reported to be required for efficient NDV replication in human cancer cells and established a link between tumourigenesis and sen

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Mossman, Karen, Hanne Ostergaard, Chris Upton, and Grant McFadden. "Myxoma virus and shope fibroma virus encode dual-specificity tyrosine/serine phosphatases which are essential for virus viability." Virology 206, no. 1 (1995): 572–82. http://dx.doi.org/10.1016/s0042-6822(95)80074-3.

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Hassanbhai, Ammar M., Meng Chee Phoon, Vincent T. Chow, and Bow Ho. "The Association of Helicobacter pylori Biofilm with Enterovirus 71 Prolongs Viral Viability and Survival." International Journal of Molecular Sciences 24, no. 19 (2023): 14500. http://dx.doi.org/10.3390/ijms241914500.

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The transition time during which a virus leaves its host and infects the next susceptible host is critical for virus survival. Enterovirus 71 (EV71) is stable in aqueous environments, but its molecular interactions with bacteria and their biofilms are not well-established. Helicobacter pylori is a highly successful gut bacterial pathogen, with its capacity to form biofilms being linked to its transmission. Given that both are gut-associated microbes, we hypothesized that biofilms formed by H. pylori may play a significant role in the survival of EV71 in the external environment. In this study,

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Fay, Andre P. "Impact of Zika virus infection in human glioblastoma cell lines." Journal of Clinical Oncology 37, no. 15_suppl (2019): e13563-e13563. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e13563.

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e13563 Background: Glioblastoma (GBM) is the most common central nervous system (CNS) tumor. Despite available therapies survival remains poor, and new treatment strategies are needed. Oncolytic viral therapy is under investigation in GBM. Zika virus (ZV) infection has demonstrated inhibition of neuronal precursor cells proliferation in preclinical studies. This study aims to evaluate the effects of ZV infection on human glioblastoma cell lines survival. Methods: Two GBM cell lines (U138 and U 251) were infected by 2 hours with ZV using the titer of PFU/ml diluted in 1:100, 1:1000, 1: 10000. C

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Vázquez‐Salgado, Lucia, Jose G. Olveira, and Isabel Bandín. "Nervous necrosis virus viability modulation by water salinity and temperature." Journal of Fish Diseases 45, no. 4 (2022): 561–68. http://dx.doi.org/10.1111/jfd.13581.

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Samkov, A., O. Burgasova, M. Chesnokova, and I. Tyurin. "A personalized approach to discharge and follow-up of patients with COVID-19." Vrach 34, no. 9 (2023): 37–41. http://dx.doi.org/10.29296/25877305-2023-11-09.

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Criteria for laboratory screening of patients with COVID-19 in hospital settings are proposed to justify treatment, discharge and terms of dispensary follow-up. Optimal approaches are based on the determination of viral load, SARS-CoV-2 viability and serum ferritin level in the dynamics of the disease. High viral load is a criterion for an unfavorable outcome, ferritin level >200 mg/l indicates possible virus persistence. Assessment of SARS-CoV-2 virus viability substantiates a period of infectiousness in COVID-19 patients. At the same time, a longer excretion of the virus with feces is not

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Thomas, Joanne M., Mark P. Stevens, Neil Percy, and Wendy S. Barclay. "Phosphorylation of the M2 Protein of Influenza A Virus Is Not Essential for Virus Viability." Virology 252, no. 1 (1998): 54–64. http://dx.doi.org/10.1006/viro.1998.9384.

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18

SARMAH, HIRAMONI, SAMSUN NEHER, SOPHIA M. GOGOI, et al. "EFFECTIVENESS OF CRYOPRESERVED CHICKEN EMBRYO FIBROBLAST CELLS FOR PROPAGATION OF DUCK ENTERITIS VIRUS AND FOWLPOX VIRUS." Asian Journal of Microbiology, Biotechnology & Environmental Sciences 25, no. 02 (2023): 256–62. http://dx.doi.org/10.53550/ajmbes.2023.v25i02.012.

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Primary chicken embryo fibroblast (CEF) cultures are excellent model systems for in-vitro virus propagation. However, the cumbersome preparation process of CEF makes cryopreservation highly essential. Cryopreservation in Liquid Nitrogen liquid phase (-196 °C) or vapour phase (-156 °C) are frequently used methods. In the present study, efficiency of cryopreserved primary CEF cells for the propagation of Duck plague virus and Fowl pox virus was evaluated. The cells were preserved for different time periods and different cooling methodologies were employed in order to assess the effects exerted b

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19

Chan, K. H., J. S. Malik Peiris, S. Y. Lam, L. L. M. Poon, K. Y. Yuen, and W. H. Seto. "The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus." Advances in Virology 2011 (2011): 1–7. http://dx.doi.org/10.1155/2011/734690.

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The main route of transmission of SARS CoV infection is presumed to be respiratory droplets. However the virus is also detectable in other body fluids and excreta. The stability of the virus at different temperatures and relative humidity on smooth surfaces were studied. The dried virus on smooth surfaces retained its viability for over 5 days at temperatures of 22–25°C and relative humidity of 40–50%, that is, typical air-conditioned environments. However, virus viability was rapidly lost (>3 log10) at higher temperatures and higher relative humidity (e.g., 38°C, and relative humidity of &

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González-Búrquez, María de Jesús, Francisco Rodolfo González-Díaz, Carlos Gerardo García-Tovar, et al. "Comparison between In Vitro Antiviral Effect of Mexican Propolis and Three Commercial Flavonoids against Canine Distemper Virus." Evidence-Based Complementary and Alternative Medicine 2018 (August 6, 2018): 1–9. http://dx.doi.org/10.1155/2018/7092416.

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Propolis is a resin that honey bees (Apis mellifera) produce by mixing wax, exudates collected from tree shoots, pollen, and enzymes. It has been used for its biological properties against pathogenic microorganisms including those of viral origin. In the present study, we demonstrate the antiviral effect of Mexican propolis, as well as of the three commercial flavonoids (quercetin, naringenin, and pinocembrin) present in its composition, in cell cultures infected with Canine Distemper Virus. The treatments were carried out with propolis, flavonoids individually, and a mixture of the three flav

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Tresnaningtyas, Sekar Asri, Fithriyah Sjatha, and Beti Ernawati Dewi. "Infectivity and viability of dengue virus infected hepatocytes cocultured with peripheral blood mononuclear cells from a healthy subject." Medical Journal of Indonesia 29, no. 3 (2020): 260–7. http://dx.doi.org/10.13181/mji.oa.203433.

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BACKGROUND Dengue virus (DENV) can infect and replicate in monocytes, resulting in antibody-dependent enhancement. The liver is the main target of DENV, and the infection mechanisms of DENV include direct cytopathic effects (CPEs) of the virus, mitochondrial dysfunction, and effect of cellular and humoral immune factors in the liver. This study was aimed to explore the infectivity of DENV and viability of human hepatocytes using Huh 7it-1 cells cocultured with peripheral blood mononuclear cells (PBMCs). METHODS Huh 7it-1 cells were infected with dengue virus serotype-2 (DENV-2) New Guine

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Mitroshina, Elena, Tatiana Mishchenko, Alexandra Usenko, et al. "AAV-Syn-BDNF-EGFP Virus Construct Exerts Neuroprotective Action on the Hippocampal Neural Network during Hypoxia In Vitro." International Journal of Molecular Sciences 19, no. 8 (2018): 2295. http://dx.doi.org/10.3390/ijms19082295.

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Brain-derived neurotrophic factor (BDNF) is one of the key signaling molecules that supports the viability of neural cells in various brain pathologies, and can be considered a potential therapeutic agent. However, several methodological difficulties, such as overcoming the blood–brain barrier and the short half-life period, challenge the potential use of BDNF in clinical practice. Gene therapy could overcome these limitations. Investigating the influence of viral vectors on the neural network level is of particular interest because viral overexpression affects different aspects of cell metabo

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Rojas-Neyra, Aldo, Katherine Calderón, Brigith Carbajal-Lévano та ін. "Evaluation of Apoptosis and Cytotoxicity Induction Using a Recombinant Newcastle Disease Virus Expressing Human IFN-γ in Human Prostate Cancer Cells In Vitro". Biomedicines 13, № 7 (2025): 1710. https://doi.org/10.3390/biomedicines13071710.

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Background/Objectives: Prostate cancer is the second most common type of cancer diagnosed in men. Various treatments for this cancer, such as radiation therapy, surgery, and systemic therapy, can cause side effects in patients; therefore, there is a need to develop new treatment alternatives. One promising approach is virotherapy, which involves using oncolytic viruses (OVs), such as the recombinant Newcastle disease virus (rNDV). Methods: We used the lentogenic rNDV rLS1 strain (the control virus) as our backbone to develop two highly fusogenic rNDVs: rFLCF5nt (the parental virus) and rFLCF5n

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Islam, Md Koushikul, Maria Baudin, Jonas Eriksson, et al. "High-Throughput Screening Using a Whole-Cell Virus Replication Reporter Gene Assay to Identify Inhibitory Compounds against Rift Valley Fever Virus Infection." Journal of Biomolecular Screening 21, no. 4 (2016): 354–62. http://dx.doi.org/10.1177/1087057115625184.

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Rift Valley fever virus (RVFV) is an emerging virus that causes serious illness in humans and livestock. There are no approved vaccines or treatments for humans. The purpose of the study was to identify inhibitory compounds of RVFV infection without any preconceived idea of the mechanism of action. A whole-cell-based high-throughput drug screening assay was developed to screen 28,437 small chemical compounds targeting RVFV infection. To accomplish both speed and robustness, a replication-competent NSs-deleted RVFV expressing a fluorescent reporter gene was developed. Inhibition of fluorescence

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Huang, Claire Y. H., Shawn J. Silengo, Melissa C. Whiteman, and Richard M. Kinney. "Chimeric Dengue 2 PDK-53/West Nile NY99 Viruses Retain the Phenotypic Attenuation Markers of the Candidate PDK-53 Vaccine Virus and Protect Mice against Lethal Challenge with West Nile Virus." Journal of Virology 79, no. 12 (2005): 7300–7310. http://dx.doi.org/10.1128/jvi.79.12.7300-7310.2005.

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ABSTRACT Chimeric dengue serotype 2/West Nile (D2/WN) viruses expressing prM-E of WN NY99 virus in the genetic background of wild-type D2 16681 virus and two candidate D2 PDK-53 vaccine variants (PDK53-E and PDK53-V) were engineered. The viability of the D2/WN viruses required incorporation of the WN virus-specific signal sequence for prM. Introduction of two mutations at M-58 and E-191 in the chimeric cDNA clones further improved the viability of the chimeras constructed in all three D2 carriers. Two D2/WN chimeras (D2/WN-E2 and -V2) engineered in the backbone of the PDK53-E and -V viruses re

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Swardson, C. J., D. L. Lichtenstein, S. Wang, R. C. Montelaro, and G. J. Kociba. "Infection of bone marrow macrophages by equine infectious anemia virus." American Journal of Veterinary Research 58, no. 12 (1997): 1402–7. http://dx.doi.org/10.2460/ajvr.1997.58.12.1402.

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SUMMARY Objective To characterize infection of bone marrow-derived macrophages (BMDM) with equine infectious anemia virus (EIAV) by determining virus production, effects on viability, and induction of cytokines. Sample Population BMDM obtained from bone marrow of 6 clinically normal adult horses. Procedure BMDM were infected with EIAV at a multiplicity of infection of 8. Cell viability, percentage of cells with detectable viral protein, reverse transcriptase activity, and concentrations of infective virus (focus-forming units/ml), interleukin 6, and tumor necrosis factor-α were measured in cul

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Llamas, Susana, Ignacio M. Moreno, and Fernando García-Arenal. "Analysis of the viability of coat-protein hybrids between Cucumber mosaic virus and Tomato aspermy virus." Journal of General Virology 87, no. 7 (2006): 2085–88. http://dx.doi.org/10.1099/vir.0.81871-0.

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Coat-protein (CP) hybrids between Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) were engineered to analyse reported CP-associated differences between these viruses. CP portions delimited by aa 1–59, 60–148 and 149–219 were exchanged in all possible combinations within TAV RNA3. The seven possible chimeras were able to replicate in tobacco protoplasts to similar levels, but only those having residues 1–59 or 60–148 from CMV were infectious to tobacco plants, a common host for CMV and TAV, and formed stable particles. When most of the movement protein (MP) of TAV was substituted for

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Tognarelli, Eduardo I., Luisa F. Duarte, Mónica A. Farías, et al. "Heme Oxygenase-1 Expression in Dendritic Cells Contributes to Protective Immunity against Herpes Simplex Virus Skin Infection." Antioxidants 12, no. 6 (2023): 1170. http://dx.doi.org/10.3390/antiox12061170.

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Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infections are highly prevalent in the human population and produce mild to life-threatening diseases. These viruses interfere with the function and viability of dendritic cells (DCs), which are professional antigen-presenting cells that initiate and regulate the host’s antiviral immune responses. Heme oxygenase-1 (HO-1) is an inducible host enzyme with reported antiviral activity against HSVs in epithelial cells and neurons. Here, we sought to assess whether HO-1 modulates the function and viability of DCs upon infection with HSV-1 or HSV

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Wang, Huiyaxin. "The deletion of adenovirus E1B-19KD gene enhanced its cell killing ability against lung cancer cell line A549." Highlights in Science, Engineering and Technology 74 (December 29, 2023): 9–17. http://dx.doi.org/10.54097/y8076r98.

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Oncolytic Viruses immunotherapy is one of the most frequently used anti-cancer agents because it can significantly reverse local immune suppression and amplify antitumor immunity. Although drugs were already applied in cancer treatment, a new agent may open a new pathway for developing oncolytic therapy. This study intends to improve the adenoviral vector to increase its killing effect on tumor cells for effective and safe cancer gene therapy. We modified the pDC316 plasmid by adding TERT promoter, E1A, and E1B viral genes through homologous recombination and transfection. Then the virus was r

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Duc, Hoang Minh, Mark Hutchinson, Gary A. Flory, et al. "Viability of African Swine Fever Virus with the Shallow Burial with Carbon Carcass Disposal Method." Pathogens 12, no. 4 (2023): 628. http://dx.doi.org/10.3390/pathogens12040628.

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African swine fever (ASF) is a highly contagious swine disease with high mortality. In many countries, culling pigs infected and exposed to the ASF virus is mandatory to control the disease, which poses a real challenge in the disposal of large numbers of carcasses during ASF outbreaks. Shallow burial with carbon (SBC) Thanks ew mortality disposal method developed from deep burial and composting. The present study investigates the effectiveness of SBC in disposing of ASF virus-infected pigs. The real-time PCR results showed that DNA of the ASF virus was still detected in bone marrow samples on

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Finstad, Samantha L., Naomi Rosenberg, and Laura S. Levy. "Diminished Potential for B-Lymphoid Differentiation after Murine Leukemia Virus Infection In Vivo and in EML Hematopoietic Progenitor Cells." Journal of Virology 81, no. 13 (2007): 7274–79. http://dx.doi.org/10.1128/jvi.00250-07.

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ABSTRACT Infection with a recombinant murine-feline gammaretrovirus, MoFe2, or with the parent virus, Moloney murine leukemia virus, caused significant reduction in B-lymphoid differentiation of bone marrow at 2 to 8 weeks postinfection. The suppression was selective, in that myeloid potential was significantly increased by infection. Analysis of cell surface markers and immunoglobulin H gene rearrangements in an in vitro model demonstrated normal B-lymphoid differentiation after infection but significantly reduced viability of differentiating cells. This reduction in viability may confer a se

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Feron, Benedita K. L., Joachim J. Bugert, and Simon C. W. Richardson. "Pioneering siRNA-Mediated Protection of Mammalian Cells against Zika Virus (MR-766) Infection." Proceedings 50, no. 1 (2020): 45. http://dx.doi.org/10.3390/proceedings2020050045.

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Here, we present empirical data documenting the siRNA-mediated protection of cells after Zika virus (ZIKV) infection. siRNAs were designed to target well-conserved sequences across the ZIKV genome. Several delivery technologies were utilized. After the electroporation of 100 nM siRNA into human hepatocyte-derived carcinoma (Huh7) cells, the Feron Zv-2 sequence (specific to the ZIKV NS3 gene) yielded a cell viability of 150.3% ± 7.4% (SEM: n = 4) (p = 0.0004) relative to the cells treated only with the virus (33.9% ± 12%, SEM: n = 4). Furthermore, 100 nM siRNA Feron Zv-4 (specific to ZIKV 3’UTR

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Stuart, D. T., C. Upton, M. A. Higman, E. G. Niles, and G. McFadden. "A poxvirus-encoded uracil DNA glycosylase is essential for virus viability." Journal of Virology 67, no. 5 (1993): 2503–12. http://dx.doi.org/10.1128/jvi.67.5.2503-2512.1993.

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Hughes, P. J., C. Horsnell, T. Hyypiä, and G. Stanway. "The coxsackievirus A9 RGD motif is not essential for virus viability." Journal of virology 69, no. 12 (1995): 8035–40. http://dx.doi.org/10.1128/jvi.69.12.8035-8040.1995.

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Ventura, Lori M., and Richard D. Dix. "Viability of Herpes Simplex Virus Type 1 on the Applanation Tonometer." American Journal of Ophthalmology 103, no. 1 (1987): 48–52. http://dx.doi.org/10.1016/s0002-9394(14)74168-6.

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Bothner, Brian, and Gary Siuzdak. "Electrospray Ionization of a Whole Virus: Analyzing Mass, Structure, and Viability." ChemBioChem 5, no. 3 (2004): 258–60. http://dx.doi.org/10.1002/cbic.200300754.

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Tsai, Shou-Kuan, Cheng-Hsin Shih, Hui-Wen Chang, et al. "Replication of a Dog-Origin H6N1 Influenza Virus in Cell Culture and Mice." Viruses 12, no. 7 (2020): 704. http://dx.doi.org/10.3390/v12070704.

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The world’s first natural avian-origin H6N1 influenza A virus infection case in dogs was confirmed in Taiwan in 2014. The H6N1 virus in chickens has been endemic in Taiwan since 1972. Whether the dog H6N1 virus has interspecies transmission potential is the key issue we aim to understand. Following one virus passage in embryonated eggs and two further passages in MDCK cells, we obtained two virus derivatives, E01EE (PB1 739E and PB2 627E) and E01GK (PB1 739G and PB2 627K), respectively. The pathogenicity of E01EE and E01GK was investigated using plaque assay, growth dynamic analysis and cell v

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Satoto, Tri Baskoro T., Sitti Umniyati, Adi Suardipa, and Margareta Sintorini. "Effects of Temperature, Relative Humidity, and DEN-2 Virus Transovarial Infection on Viability of Aedes aegypti." Kesmas: National Public Health Journal 7, no. 7 (2013): 331. http://dx.doi.org/10.21109/kesmas.v7i7.32.

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Environmental changes influenced survival life and virus transmission of dengue virus (DEN) in a mosquito. The purpose of the present study was to define DEN-2 virus transmission dynamic and effect of temperature, relative humidity (RH), and DEN-2 virus infection on viability of Aedes aegypti (Ae. aegypti). This experimental study with pretest-posttest control group design was conducted at the Laboratory of Center for Tropical Medicine, Faculty of Medicine, Gadjah Mada University (UGM), Yogyakarta. Seventh daysold female Ae.aegypti (F0) were infected DEN-2 viaoral membrane and kept until F2 ge

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Werdin, Ronald E., Trevor R. Ames, Sagar M. Goyal, and Gordon P. DeVries. "Diagnostic Investigation of Bovine Viral Diarrhea Infection in a Minnesota Dairy Herd." Journal of Veterinary Diagnostic Investigation 1, no. 1 (1989): 57–61. http://dx.doi.org/10.1177/104063878900100116.

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Bovine viral diarrhea (BVD) virus infection was diagnosed in neonatal calves with enteritis. Successful diagnostic procedures included direct immunofluorescence of frozen tissue sections, histopathology, and virus isolation. Virus isolation from buffy coats and serum was successful in detecting infected animals, whereas direct immunofluorescence of buffy coat samples was found to be less reliable. Virus was not isolated from any fecal samples. Booster vaccinations and the culling of animals shedding virus resulted in improved calf viability in this herd. It is suggested that procedures for the

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Rius-Salvador, Marina, Maria Jesús García-Múrria, Luciana Rusu, et al. "Cetylpyridinium chloride and chlorhexidine show antiviral activity against Influenza A virus and Respiratory Syncytial virus in vitro." PLOS ONE 19, no. 2 (2024): e0297291. http://dx.doi.org/10.1371/journal.pone.0297291.

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Background The oral cavity is the site of entry and replication for many respiratory viruses. Furthermore, it is the source of droplets and aerosols that facilitate viral transmission. It is thought that appropriate oral hygiene that alters viral infectivity might reduce the spread of respiratory viruses and contribute to infection control. Materials and methods Here, we analyzed the antiviral activity of cetylpyridinium chloride (CPC), chlorhexidine (CHX), and three commercial CPC and CHX-containing mouthwash preparations against the Influenza A virus and the Respiratory syncytial virus. To d

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LIMA, MAYKY FRANCLEY PEREIRA DE, WELDER DE ARAÚJO RANGEL LOPES, MARIA ZULEIDE DE NEGREIROS, FRANCISCO VILELA RESENDE, ANTÔNIA TAMIRES MONTEIRO BESSA, and LEILSON COSTA GRANGEIRO. "CLOVE-SEED SIZE AND HEALTH AND PLANT SPACING ON THE VIABILITY OF GARLIC CROPS." Revista Caatinga 34, no. 3 (2021): 559–69. http://dx.doi.org/10.1590/1983-21252021v34n307rc.

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ABSTRACT Garlic is a vegetable that has a high economic importance for Brazil. However, despite increases in garlic bulb production in the last years, Brazil is still dependent on imported garlic to meet the national demand. Thus, proper management practices and the use of virus-free clove-seeds are promising alternatives to ensure a high productivity and profitability. Thus, the objective of this work was to evaluate production characteristics and profitability of conventional and virus-free garlic crops as a function of different clove-seed sizes and plant spacings. Two experiments were cond

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Xu, Fangling, Xiaodong Liang, Robert B. Tesh, and Shu-Yuan Xiao. "Characterization of cell-death pathways in Punta Toro virus-induced hepatocyte injury." Journal of General Virology 89, no. 9 (2008): 2175–81. http://dx.doi.org/10.1099/vir.0.2008/001644-0.

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Punta Toro virus (PTV; genus Phlebovirus, family Bunyaviridae) causes apoptosis of hepatocytes in vivo in experimentally infected hamsters and in vitro in cultured HepG2 cells. Screening for expression of apoptosis-related genes has shown alterations in the genes for tumour necrosis factor-α (TNF-α) and the TNF receptor family. This study examined the roles of the TNF receptor-related extrinsic pathway and the Bcl-2 family-associated mitochondrial pathway in PTV-induced cell death. The effects of caspase inhibitors (caspIs) and TNF on cellular viability, virus replication, and morphological an

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Shin, Dong-Jun, Min Jae Kim, Joon-Gyu Min, and Kwang Il Kim. "Development of viability-quantitative PCR with propidium monoazide for assessment of white spot syndrome virus structural integrity and viability." Aquaculture 602 (May 2025): 742317. https://doi.org/10.1016/j.aquaculture.2025.742317.

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Kaul, Artur, Ilka Woerz, Philip Meuleman, Geert Leroux-Roels, and Ralf Bartenschlager. "Cell Culture Adaptation of Hepatitis C Virus and In Vivo Viability of an Adapted Variant." Journal of Virology 81, no. 23 (2007): 13168–79. http://dx.doi.org/10.1128/jvi.01362-07.

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ABSTRACT Production of infectious hepatitis C virus in cell culture has become possible because of the unique properties of the JFH1 isolate. However, virus titers are rather low, limiting the utility of this system. Here we describe the generation of cell culture-adapted JFH1 variants yielding higher titers of infectious particles and enhanced spread of infection in cultured cells. Sequence analysis of adapted genomes revealed a complex pattern of mutations that differed in two independent experiments. Adaptive mutations were observed both in the structural and in the nonstructural regions, w

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Jensen, Catherine, and F. Brent Johnson. "Comparison of various transport media for viability maintenance of herpes simplex virus, respiratory syncytial virus, and adenovirus." Diagnostic Microbiology and Infectious Disease 19, no. 3 (1994): 137–42. http://dx.doi.org/10.1016/0732-8893(94)90055-8.

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Gibbons, Sean M., Tara Schwartz, Jennifer Fouquier, et al. "Ecological Succession and Viability of Human-Associated Microbiota on Restroom Surfaces." Applied and Environmental Microbiology 81, no. 2 (2014): 765–73. http://dx.doi.org/10.1128/aem.03117-14.

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ABSTRACTHuman-associated bacteria dominate the built environment (BE). Following decontamination of floors, toilet seats, and soap dispensers in four public restrooms,in situbacterial communities were characterized hourly, daily, and weekly to determine their successional ecology. The viability of cultivable bacteria, following the removal of dispersal agents (humans), was also assessed hourly. A late-successional community developed within 5 to 8 h on restroom floors and showed remarkable stability over weeks to months. Despite late-successional dominance by skin- and outdoor-associated bacte

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Xie, Dafei, Lu Han, Yifu Luo, et al. "Exploring the associations of host genes for viral infection revealed by genome-wide RNAi and virus–host protein interactions." Molecular BioSystems 11, no. 9 (2015): 2511–19. http://dx.doi.org/10.1039/c5mb00309a.

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Genome-wide RNA interference screens have greatly facilitated the identification of essential host factors (EHFs) for viral infections, whose knockdown effects significantly influence virus replication but not host cell viability.

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Shevchenko, O. V., A. V. Kharina, H. O. Snihur, et al. "Virus Occurrence and Survival in Reusable Resources: A Minireview." Mikrobiolohichnyi Zhurnal 84, no. 4 (2023): 98–104. http://dx.doi.org/10.15407/microbiolj84.04.098.

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This work covers important aspects of the occurrence and viability of various viruses in the two most common reusable waste resources: wastewater and biomass waste. Detection of human, bacterial and plant viruses in these wastes are summarized. Historically, human viruses have been monitored in wastewater for decades. Evidence suggests that wastewater mostly contains fecal-orally transmitted viruses, which are abundant and diverse. Recently, an increasing occurrence of SARS-CoV2 in sewage water with the spreading epidemics has been confirmed but lacking biological proof of infectivity yet. Bes

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Ahlawat, Ajit, Sumit Kumar Mishra, Hartmut Herrmann, et al. "Impact of Chemical Properties of Human Respiratory Droplets and Aerosol Particles on Airborne Viruses’ Viability and Indoor Transmission." Viruses 14, no. 7 (2022): 1497. http://dx.doi.org/10.3390/v14071497.

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The airborne transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as a potential pandemic challenge, especially in poorly ventilated indoor environments, such as certain hospitals, schools, public buildings, and transports. The impacts of meteorological parameters (temperature and humidity) and physical property (droplet size) on the airborne transmission of coronavirus in indoor settings have been previously investigated. However, the impacts of chemical properties of viral droplets and aerosol particles (i.e., chemical composition and acidity (pH))

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Damian C. Odimegwu and Agatha I. Onah. "The anti-respiratory syncytial virus activity of methanol leaf extract of Aspilia africana." International Journal of Research in Pharmaceutical Sciences 11, no. 2 (2020): 2326–31. http://dx.doi.org/10.26452/ijrps.v11i2.2209.

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Respiratory syncytial virus (RSV), to date, is still the most frequent cause of infantile and childhood cause of viral lower respiratory tract illness on a global scale, and at the same time, causing significant impact in the elderly. Presently, there is no licensed vaccine or safe and specific antiviral agent that could be used against the virus. Medicinal plants are known to be a promising source for the discovery of utilizable anti-RSV treatments. In this present study, we screened the anti-RSV and cell cytotoxicity activities of methanol extract of A. Africana by virus plaque reduction and

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