Academic literature on the topic 'Vistec Electron Beam GmbH'

Imaging of fast frozen samples is the most direct approach for electron microscopy of biological specimen in a defined physiological state. It prevents chemical fixation and drying artifacts. High pressure freezing allows for ice-crystal-free cryo-fixation of tissue pieces up to a thickness of 200 urn and a diameter of 2 mm without prefixation. Such a frozen disc, however, is not directly amenable to electron microscopic observation: The structures of interest have to be made amenable to the electron beam, and the structures of interest must produce enough contrast to be recognized in the electron microscope. This can be achieved by freeze fracturing, cryo-sectioning or freeze substitution.The figures show high pressure frozen bakers yeast saccharomyces cerevisiae in the cryo-SEM (Figures 1 and 2) and after freeze substitution in the TEM (Figure 3). For high pressure freezing either a Bal-Tec HPM 010 (Princ. of Liechtenstein; Figures 1 and 2), or a Wohlwend HPF (Wohlwend GmbH, Sennwald, Switzerland; Figure 3) were used.

Field emission electron microscopes operating at 200kV or 300kV and incorporating aberration correctors for either the incident electron probe or for the primary aberrations of the objective lens (OL) are currently under development for several laboratories in the world. OL-corrected instruments require monochromators for the electron beam, built into the electron gun prior to the accelerating stages, in order to optimize the contrast transfer characteristics of the objective lens to push the instrumental resolution limit to well beyond 0.1nm. This will allow the point resolution limit as controlled by the correction of spherical aberration Cs to potentially extend to the instrumental limit of better than 0.1nm. Figure 1 shows the contrast transfer characteristics of a Cs-corrected 200kV TEM, both without and with a beam monochromator.Dedicated STEM instruments such as the 300kV VG-603 and lOOkV VG-501 at Oak Ridge National Laboratory, and other VG instruments at Cornell University and IBM Co. are also being adapted (by Nion Co., Kirkland, WA) to incorporate aberration correctors for the incident probe. The aim is to improve the resolution of the VG-603 instrument in dark-field imaging mode, for example, from 0.13nm to 0.05nm. in another ORNL project, the High Temperature Materials Laboratory has contracted JEOL Ltd. to construct a STEM-TEM instrument with a probe corrector designed and built by CEOS GmbH (Heidelberg, Germany).

Pretreatment on lignocellulosic biomass prior to extraction of biomaterials, degradation of bioproduct, or production of biomaterial/bioproduct/biofuel, crucially influences the intended outcomes. The pretreatment of oil palm fronds (OPF), one of the most abundant agriculture residues in Malaysia, can be conducted based on the need of the methodology, either for small, lab, pilot, or industrial scales. In this article, examples of reactors for the pretreatment for instance microreactor (Bioshake iQ), conical shake flask, and mini-cylindrical reactor scale (fabricated) as well as the monitoring bioreactor (BlueSens Monitoring GmbH) reactor system dedicated for fermentation process using the outcome material from pretreatment process, are presented. All pretreatment trials with ionic liquid (IL) of 1-ethyl-3-methylimidazolium acetate [EMIM]Ac on OPF were conducted with a scaling-up strategy from micro-to-macro to fabricated reactors, monitoring Crystallinity Index (CrI) and Lateral Order Index (LOI). Electron beam irradiation pretreatment using 1000kGy was also tested in macroscale mode for CrI and LOI. Effectiveness of approximately 23 to 37% of CrI via microreactor experiments using 50, 70, and 90% v/v of [EMIM]Ac and at a temperature of 99oC was observed. Higher concentration of IL and temperature with nearly insignificance of solid loading of OPF in reaction liquid to the increase of the amorphous level of OPF was reported by macroscale mode in the 570-mL fabricated reactor. A short oxygen uptake rate (OUR) phase was observed in a 500-mL BlueSens shake flask with the real-time monitoring systems for 45-mL working volume, a nearly 10% of the total reactor volume for saccharification-fermentation using Escherichia coli K011 ATCC 55124 on approximately 2.22% w/v pretreated OPF from macroscale mode. Various data examples from these micro-to-macro scales including in a fabricated reactor system mode are crucially needed for further observations prior to pilot or industrial scales, needing a systematic data collection to be simulated and investigated in the future.

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Investigation of the primary breakup region of gasoline sprays is important for future nozzle development. It improvesthe principal understanding of inner nozzle flow and spray breakup. It also allows validating and developingCFD models. Due to the high optical density common measurement techniques like Phase Doppler Anemometryreach their limit in optical dense sprays as in the primary breakup region. High Speed X-Ray Imaging is capable tomeasure 2D velocity distributions directly at the spray hole exit. For generating the intense X-Ray beam the synchrotronAdvanced Photon Source at Argonne National Laboratory is used. Passing through the spray the X-Raybeam is changed by two different physical principles: absorption and phase contrast. Absorption can be applied tomeasure the density of the spray. Phase contrast is used to visualize the borders of droplets and ligaments withhigh contrast. The accelerated electron bunches inside the synchrotron have a constant period length to each other.This leads to an accurate pulsed X-Ray beam (periodicity: 68 ns). The use of multi exposure with very short X-Raypulses (17 ns) shows the traveled distance of the spray droplets and ligaments. The spray speed (150-250 m/s) iscalculated by dividing these distances with the time gap between two X-Ray pulses. The X-Ray measured densitydistributions and velocity distributions are combined to calculate the spray force rate. The so gained force rate isvalidated with a spray force measurement performed at the Spray Momentum Test Bench (SMTB) at ContinentalAutomotive GmbH. The study is focusing on the measurement setup of High Speed X-Ray Imaging at ArgonneNational Laboratory and the evaluation algorithms.DOI: http://dx.doi.org/10.4995/ILASS2017.2017.4598