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1

SAVU, Sergiu, Liliana Lucia TOMOIAGA, and Veronica Sanda CHEDEA. "Ecological Microclimate Influence on Grapevine Phomopsis viticola Attack Frequency in Aiud-Ciumbrud Vineyards." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture 77, no. 2 (2020): 64. http://dx.doi.org/10.15835/buasvmcn-hort:2020.0026.

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Phomopsis viticola (Sacc.) Sacc. (syn. Cryptosporella viticola Shear, Diaporthe viticola Nitschke, Diplodia viticola Desm, Fusicoccum viticolum Reddick, Phoma flaccida Viala & Ravaz, Phoma viticola Sacc.), is the causal agent of the grapevine disease named ‘Phomopsis cane’ and ‘Leaf spot’ in the U.S.A. or ‘Excoriose’ in Europe. This study aims to evaluate the influence of the microclimate on Phomopsis viticola attack in Aiud-Ciumbrud vineyards. The observations were done on four plots in Aiud-Ciumbrud vineyards in the period March-August 2020. Up to the end of August 2020, ten treatments w
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2

Rawnsley, B., T. J. Wicks, E. S. Scott, and B. E. Stummer. "Diaporthe perjuncta Does Not Cause Phomopsis Cane and Leaf Spot Disease of Grapevine in Australia." Plant Disease 88, no. 9 (2004): 1005–10. http://dx.doi.org/10.1094/pdis.2004.88.9.1005.

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In Australia, Diaporthe perjuncta (formerly known as Phomopsis taxon 1) and Phomopsis viticola (Phomopsis taxon 2) have been associated with Phomopsis cane and leaf spot of grapevine. Although P. viticola causes distinct leaf spots, as well as lesions on shoots and canes, the pathogenicity of D. perjuncta is relatively unknown. The pathogenicity of D. perjuncta and P. viticola was studied in relation to symptom expression and bud loss. Only P. viticola caused brown-black, longitudinal, necrotic lesions on stem tissue and leaf spots characteristic of the disease, whereas both D. perjuncta and P
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3

Úrbez-Torres, J. R., F. Peduto, R. J. Smith, and W. D. Gubler. "Phomopsis Dieback: A Grapevine Trunk Disease Caused by Phomopsis viticola in California." Plant Disease 97, no. 12 (2013): 1571–79. http://dx.doi.org/10.1094/pdis-11-12-1072-re.

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Field surveys recently conducted in California and in other grape-growing regions in the United States showed Phomopsis viticola to be one of the most prevalent fungi isolated from grapevine perennial cankers in declining vines. The current study has not only confirmed the presence of P. viticola from grapevine cankers in California but also has for the first time revealed the occurrence of Diaporthe ambigua, D. eres, and D. neotheicola in symptomatic grapevine wood in California by means of morphological studies and multi-gene sequence analysis. Pathogenicity trials conducted on mature cordon
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4

Rouxel, Mélanie, Pere Mestre, Anton Baudoin, et al. "Geographic Distribution of Cryptic Species of Plasmopara viticola Causing Downy Mildew on Wild and Cultivated Grape in Eastern North America." Phytopathology® 104, no. 7 (2014): 692–701. http://dx.doi.org/10.1094/phyto-08-13-0225-r.

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The putative center of origin of Plasmopara viticola, the causal agent of grape downy mildew, is eastern North America, where it has been described on several members of the family Vitaceae (e.g., Vitis spp., Parthenocissus spp., and Ampelopsis spp.). We have completed the first large-scale sampling of P. viticola isolates across a range of wild and cultivated host species distributed throughout the above region. Sequencing results of four partial genes indicated the presence of a new P. viticola species on Vitis vulpina in Virginia, adding to the four cryptic species of P. viticola recently r
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5

Makarkina, Marina V., Sergey V. Tokmakov, and Elena T. Ilnitskaya. "A study of the genetic polymorphism of Plasmopara viticola in the vineyards of the Krasnodar Territory." BIO Web of Conferences 23 (2020): 02005. http://dx.doi.org/10.1051/bioconf/20202302005.

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Plasmopara viticola oomycete is a seasonal pathogen that causes one of the most harmful diseases of the grapevine – downy mildew. The study of the biodiversity of Plasmopara viticola in various zones of viticulture has fundamental goals, as well as practical ones, as it is important for understanding the epidemiological cycle of P. viticola and for refining disease prediction models. The purpose of the work is to study the genetic diversity of P. viticola in the vineyards of the Krasnodar Territory, including on the grape varieties with different levels of resistance to downy mildew. The study
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6

Aoki, Yoshinao, Yumi Kawagoe, Nozomi Fujimori, Sayumi Tanaka, and Shunji Suzuki. "Monitoring of a Single Point Mutation in the PvCesA3 Allele Conferring Resistance to Carboxylic Acid Amide Fungicides in Plasmopara viticola Populations In Yamanashi Prefecture, Japan." Plant Health Progress 16, no. 2 (2015): 84–87. http://dx.doi.org/10.1094/php-rs-14-0041.

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The use of the carboxylic acid amide (CAA) fungicide mandipropamid to manage grapevine downy mildew in vineyards in Japan has been increasing since 2010, because of widespread quinone outside inhibitor fungicide resistance in the Plasmopara viticola population. However, CAA fungicide resistance in P. viticola is becoming a serious problem worldwide. In 2013, we monitored for the presence of a single point mutation at codon 1105 of the cellulose synthase gene PvCesA3, which confers resistance to mandipropamid in P. viticola samples collected from four vineyards in Yamanashi prefecture in Japan.
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7

Ungureanu, Camelia, Liliana Cristina Soare, Diana Vizitiu, Mirela Calinescu, Irina Fierascu, and Radu Claudiu Fierascu. "Isolation of Plasmopara viticola from Grapevine Leaves." Proceedings 29, no. 1 (2019): 34. http://dx.doi.org/10.3390/proceedings2019029034.

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8

Peixoto, Ana Rosa, Rosa de Lima Ramos Mariano, and Ivanise Oliveira Viana. "Meio semi-seletivo para isolamento de Xanthomonas campestris pv. viticola." Ciência Rural 36, no. 4 (2006): 1317–20. http://dx.doi.org/10.1590/s0103-84782006000400045.

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O cancro bacteriano causado por Xanthomonas campestris pv. viticola é a fitobacteriose mais importante da videira no Submédio São Francisco. O isolamento de X. campestris pv. viticola de tecidos vegetais infectados é dificultado pela presença de contaminantes bacterianos, entre os quais Microbacterium barkeri. Objetivando-se a formulação de meio de cultura semi-seletivo, 22 isolados de X. campestris pv. viticola foram testados com relação a 30 antibióticos. O meio semi-seletivo NYDAM (extrato de carne 3, peptona 5, glicose 10, extrato de levedura 5, ágar 18 e ampicilina 0,1 em g L-1) inibiu M.
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9

Wong, F. P., H. N. Burr, and W. F. Wilcox. "Heterothallism in Plasmopara viticola." Plant Pathology 50, no. 4 (2001): 427–32. http://dx.doi.org/10.1046/j.1365-3059.2001.00573.x.

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10

Aoki, Yoshinao, Maki Hashimoto, and Shunji Suzuki. "Emergence of Single Point Mutation in PvCesA3, Conferring Resistance to CAA Fungicides, in Plasmopara viticola Populations in Japan." Plant Health Progress 14, no. 1 (2013): 51. http://dx.doi.org/10.1094/php-2013-0729-01-br.

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As far as we know, there is no report of fungicide-resistant Plasmopara viticola in Japan. In the present study, we detected a single point mutation in the PvCesA3 of P. viticola sampled from a Japanese vineyard, which conferred resistance to mandipropamid. The emergence of the mandipropamid-resistant PvCesA3 allele may lead to the spread of mandipropamid-resistant P. viticola in vineyards all over Japan in the near future. Accepted for publication 23 April 2012. Published 29 July 2013.
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11

Musetti, R., A. Vecchione, L. Stringher, et al. "Inhibition of Sporulation and Ultrastructural Alterations of Grapevine Downy Mildew by the Endophytic Fungus Alternaria alternata." Phytopathology® 96, no. 7 (2006): 689–98. http://dx.doi.org/10.1094/phyto-96-0689.

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One hundred twenty-six endophytic microorganisms isolated from grapevine leaves showing anomalous symptoms of downy mildew were tested on grapevine leaf disks as biocontrol agents against Plasmopara viticola. Among the 126 microorganisms, only five fungal isolates completely inhibited the sporulation of P. viticola; all of them were identified as Alternaria alternata. Ultrastructural analyses were carried out by transmission electron microscopy to observe cellular interactions between P. viticola and A. alternata in the grapevine leaf tissue. Cytological observations indicated that, even witho
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12

Cruz-Silva, Ana, Andreia Figueiredo, and Mónica Sebastiana. "First Insights into the Effect of Mycorrhizae on the Expression of Pathogen Effectors during the Infection of Grapevine with Plasmopara viticola." Sustainability 13, no. 3 (2021): 1226. http://dx.doi.org/10.3390/su13031226.

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Grapevine (Vitis vinifera L.), widely used for berry and wine production, is highly susceptible to the pathogenic oomycete Plasmopara viticola, the etiological agent of grapevine downy mildew disease. The method commonly used to prevent and control P. viticola infection relies on multiple applications of chemical fungicides. However, with European Union goals to lower the usage of such chemicals in viticulture there is a need to develop new and more sustainable strategies. The use of beneficial microorganisms with biocontrol capabilities, such as the arbuscular mycorrhizal fungi (AMF), has bee
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13

Aoki, Yoshinao, Arisa Usujima, and Shunji Suzuki. "High night temperature promotes downy mildew in grapevine via attenuating plant defence response and enhancing early Plasmopara viticola infection." Plant Protection Science 57, No. 1 (2020): 21–30. http://dx.doi.org/10.17221/92/2020-pps.

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The night temperature is one of the critical environmental factors affecting the grape berry quality. The objective of this study was to clarify whether a high night temperature promotes downy mildew on grapevines. The high night temperature conditions suppressed the gene expression of the pathogenesis-related proteins in the grapevine cultured cells and grapevine seedlings compared with the control night temperature conditions. The Plasmopara viticola colony formation on the leaves of the seedlings exposed to the control night temperature conditions became slightly noticeable on day 5 after i
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14

Toffolatti, Silvia Laura, Gabriella De Lorenzis, Matteo Brilli, et al. "Novel Aspects on The Interaction Between Grapevine and Plasmopara viticola: Dual-RNA-Seq Analysis Highlights Gene Expression Dynamics in The Pathogen and The Plant During The Battle For Infection." Genes 11, no. 3 (2020): 261. http://dx.doi.org/10.3390/genes11030261.

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Mgaloblishvili, a Vitis vinifera cultivar, exhibits unique resistance traits against Plasmopara viticola, the downy mildew agent. This offers the unique opportunity of exploring the molecular responses in compatible and incompatible plant-pathogen interaction. In this study, whole transcriptomes of Mgaloblishvili, Pinot noir (a V. vinifera susceptible cultivar), and Bianca (a resistant hybrid) leaves, inoculated and non-inoculated with the pathogen, were used to identify P. viticola effector-encoding genes and plant susceptibility/resistance genes. Multiple effector-encoding genes were identif
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15

Luis, P., A. Gauthier, S. Trouvelot, B. Poinssot, and P. Frettinger. "Identification of Plasmopara viticola Genes Potentially Involved in Pathogenesis on Grapevine Suggests New Similarities Between Oomycetes and True Fungi." Phytopathology® 103, no. 10 (2013): 1035–44. http://dx.doi.org/10.1094/phyto-06-12-0121-r.

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Plant diseases caused by fungi and oomycetes result in significant economic losses every year. Although phylogenetically distant, these organisms share many common features during infection. We identified genes in the oomycete Plasmopara viticola that are potentially involved in pathogenesis in grapevine by using fungal databases and degenerate primers. Fragments of P. viticola genes encoding NADH-ubiquinone oxidoreductase (PvNuo), laccase (PvLac), and invertase (PvInv) were obtained. PvNuo was overexpressed at 2 days postinoculation (dpi), during the development of the first hyphal structures
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16

Colcol, Jeneylyne F., and Anton B. Baudoin. "Sensitivity of Erysiphe necator and Plasmopara viticola in Virginia to QoI Fungicides, Boscalid, Quinoxyfen, Thiophanate Methyl, and Mefenoxam." Plant Disease 100, no. 2 (2016): 337–44. http://dx.doi.org/10.1094/pdis-01-15-0012-re.

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The sensitivity of downy mildew (DM, Plasmopara viticola) and powdery mildew (PM, Erysiphe necator) of grape (Vitis sp.) to commonly used nondemethylation inhibitor, single-site fungicides in and near Virginia was determined from 2005 to 2007, with more limited additional sampling in subsequent years. In grape leaf disc bioassays, 92% of the P. viticola isolates were quinone outside inhibitor (QoI, azoxystrobin) resistant but none were resistant to mefenoxam. In all, 82% of the E. necator isolates were QoI resistant. Most of the QoI-resistant P. viticola and E. necator isolates contained >9
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17

Taylor, Andrew S., Brian J. Knaus, Niklaus J. Grünwald, and Treena Burgess. "Population Genetic Structure and Cryptic Species ofPlasmopara viticolain Australia." Phytopathology® 109, no. 11 (2019): 1975–83. http://dx.doi.org/10.1094/phyto-04-19-0146-r.

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Downy mildew of grape caused by Plasmopara viticola is a global pathogen of economic importance to commercial viticulture. In contrast to populations in the northern hemisphere, few studies have investigated the population biology, genetic diversity, and origin of the pathogen in Australian production systems. DNA was extracted from 381 P. viticola samples from Vitis vinifera and alternate hosts collected via fresh and herbarium leaves from populations within Australia and Whatman FTA cards from North America, Brazil, and Uruguay. A total of 32 DNA samples were provided from a French populatio
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18

Schaubschläger, Wolfgang Wenzel, Wolf-Meinhard Becker, Gertraud Mazur, and Martin Gödde. "Occupational sensitization to Plasmopara viticola." Journal of Allergy and Clinical Immunology 93, no. 2 (1994): 457–63. http://dx.doi.org/10.1016/0091-6749(94)90354-9.

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19

Monteiro, Sara, Mahmoud Barakat, Maria A. Piçarra-Pereira, Artur R. Teixeira, and Ricardo B. Ferreira. "Osmotin and Thaumatin from Grape: A Putative General Defense Mechanism Against Pathogenic Fungi." Phytopathology® 93, no. 12 (2003): 1505–12. http://dx.doi.org/10.1094/phyto.2003.93.12.1505.

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Little information is available concerning the expression of pathogenesis-related (PR) proteins in grapevine (Vitis vinifera) and their effect properties on the major fungal pathogens of grape. A systematic study was performed on the effect of total or individual grape proteins on mycelial growth, spore germination, and germ tube growth of Uncinula necator, Phomopsis viticola, and Botrytis cinerea. Two proteins, identified as PR proteins by immunological methods and by N-terminal sequencing as osmotin and thaumatin-like protein, exhibited strong antifungal activities in vitro, blocking the gro
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20

Baumgartner, Kendra, Phillip T. Fujiyoshi, Renaud Travadon, Lisa A. Castlebury, Wayne F. Wilcox, and Philippe E. Rolshausen. "Characterization of Species of Diaporthe from Wood Cankers of Grape in Eastern North American Vineyards." Plant Disease 97, no. 7 (2013): 912–20. http://dx.doi.org/10.1094/pdis-04-12-0357-re.

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In eastern North America, Phomopsis cane and leaf spot, caused by Phomopsis viticola, is a foliar disease of grape but, in the Mediterranean climate of western North America, P. viticola is primarily associated with wood cankers, along with other Diaporthe spp. To determine the identity of wood-infecting Diaporthe spp. in eastern North America, 65 isolates were cultured from 190 wood-canker samples from 23 vineyards with a history of Phomopsis cane and leaf spot. Identification of 29 representative isolates was based initially on morphology, followed by phylogenetic analyses of DNA sequences o
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Úrbez-Torres, J. R., W. D. Gubler, and J. Luque. "First Report of Botryosphaeria iberica and B. viticola Associated with Grapevine Decline in California." Plant Disease 91, no. 6 (2007): 772. http://dx.doi.org/10.1094/pdis-91-6-0772c.

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Grapevine decline symptoms in California include dead spurs and cordon and trunk dieback due to canker formation in the vascular tissue. Seven Botryosphaeria spp. are known to be associated with grapevine cankers in California, viz. Botryosphaeria australis, B. dothidea, B. lutea, B. obtusa, B. parva, B. rhodina, and B. stevensii (3). Recently, B. iberica and B. viticola also were isolated from grapevine cankers in a field survey that was conducted throughout California. Identification was based on morphological comparisons along with DNA analyses with previously identified isolates from Spain
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22

Wicks, T., TC Lee, and J. Overton. "Sensitivity of Australian isolates of Plasmopara viticola to acylanaline fungicides." Australian Journal of Experimental Agriculture 27, no. 4 (1987): 601. http://dx.doi.org/10.1071/ea9870601.

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A floating leaf disk technique was used to test the sensitivity of Australian isolates of Plasmopara viticola to 4 acylanaline fungicides, phosethyl-A1 and phosphorous acid. Sporulation of all 15 isolates was completely inhibited by 1 mg/L of metalaxyl. Benalaxyl, oxadixyl and ofurace at 10 mg/L and phosethyl-A1 and phosphorous acid at 100 mg/L also completely inhibited sporulation of each of the isolates tested for each fungicide. These results indicate that acylanaline insensitive isolates of P. viticola are either not present or are not widely distributed in grape growing areas of Australia
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23

Massi, Federico, Stefano F. F. Torriani, Lorenzo Borghi, and Silvia L. Toffolatti. "Fungicide Resistance Evolution and Detection in Plant Pathogens: Plasmopara viticola as a Case Study." Microorganisms 9, no. 1 (2021): 119. http://dx.doi.org/10.3390/microorganisms9010119.

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The use of single-site fungicides to control plant pathogens in the agroecosystem can be associated with an increased selection of resistance. The evolution of resistance represents one of the biggest challenges in disease control. In vineyards, frequent applications of fungicides are carried out every season for multiple years. The agronomic risk of developing fungicide resistance is, therefore, high. Plasmopara viticola, the causal agent of grapevine downy mildew, is a high risk pathogen associated with the development of fungicide resistance. P. viticola has developed resistance to most of
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24

da Gama, Marco Aurélio Siqueira, Rosa de Lima Ramos Mariano, Wilson José da Silva Júnior, et al. "Taxonomic Repositioning of Xanthomonas campestris pv. viticola (Nayudu 1972) Dye 1978 as Xanthomonas citri pv. viticola (Nayudu 1972) Dye 1978 comb. nov. and Emendation of the Description of Xanthomonas citri pv. anacardii to Include Pigmented Isolates Pathogenic to Cashew Plant." Phytopathology® 108, no. 10 (2018): 1143–53. http://dx.doi.org/10.1094/phyto-02-18-0037-r.

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Grapevine bacterial canker, which is caused by Xanthomonas campestris pv. viticola, is one of the most important grapevine diseases in the northeastern region of Brazil. This disease causes severe damage and represents a high potential risk to the development of Brazilian viticulture. In turn, pigmented isolates pathogenic to cashew plant, making cashew fruit unfit for sale, also have been detected in Northeastern Brazil. Given that the taxonomic position of these bacteria is unclear, the multilocus sequence analysis (MLSA) technique, average nucleotide identity (ANI) values and tetranucleotid
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25

Campbell, Sarah E., Phillip M. Brannen, Harald Scherm, and Marin T. Brewer. "Fungicide Sensitivity Survey of Plasmopara viticola Populations in Georgia Vineyards." Plant Health Progress 21, no. 4 (2020): 256–61. http://dx.doi.org/10.1094/php-05-20-0039-rs.

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Grapevine downy mildew, caused by Plasmopara viticola, is among the most damaging diseases of grapes globally and in the viticultural regions of Georgia (U.S.A.). Although management of this disease typically involves fungicide applications, resistance development in P. viticola can render chemical management ineffective. The objective of this study was to survey fungicide sensitivities of P. viticola populations in vineyards across three regions in Georgia. Samples were collected from eight vineyards in 2017 and four in 2018 and tested phenotypically for sensitivity to quinone outside inhibit
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26

Valsesia, G., D. Gobbin, A. Patocchi, A. Vecchione, I. Pertot, and C. Gessler. "Development of a High-Throughput Method for Quantification of Plasmopara viticola DNA in Grapevine Leaves by Means of Quantitative Real-Time Polymerase Chain Reaction." Phytopathology® 95, no. 6 (2005): 672–78. http://dx.doi.org/10.1094/phyto-95-0672.

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Plasmopara viticola is a strictly biotrophic oomycete that causes downy mildew, which is one of the most important grapevine diseases. Control of the disease is most often achieved by fungicide applications, which may have severe environmental consequences. Therefore, alternative control strategies based on biocontrol agents (BCAs) are currently in development. Thousands of potential BCAs have to be screened for their antagonist efficacy against Plasmopara viticola. Evaluation of their effect on the pathogen can be achieved by detecting the amount of P. viticola DNA in leaves treated with pote
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Schilder, A. M. C., O. Erincik, L. Castlebury, A. Rossman, and M. A. Ellis. "Characterization of Phomopsis spp. Infecting Grapevines in the Great Lakes Region of North America." Plant Disease 89, no. 7 (2005): 755–62. http://dx.doi.org/10.1094/pd-89-0755.

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Seventy-five isolates of Phomopsis were obtained from grapes (Vitis spp.) with Phomopsis cane and leaf spot symptoms in Ohio, Michigan, New York, Pennsylvania, Maryland, and Ontario (Canada). Four isolates from California and one from Europe were also included in the study. Isolates were grouped on the basis of DNA sequences from intron regions in the translation elongation factor 1-α and calmodulin genes. According to DNA sequence comparisons with the type isolate, all isolates except two (OH-48 and CAL-5) were determined to be P. viticola, confirming the etiology of this disease in the Great
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28

McKirdy, S. J., I. T. Riley, I. J. Cameron, and P. A. Magarey. "First Report of Grapevine Downy Mildew (Plasmopara viticola) in Commercial Viticulture in Western Australia." Plant Disease 83, no. 3 (1999): 301. http://dx.doi.org/10.1094/pdis.1999.83.3.301d.

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Despite the suitability of climate, Western Australia was one of the few grape (Vitis vinifera L.) growing areas free of grapevine downy mildew (Plasmopara viticola (Berk. & M. A. Curtis) Berl. & De Toni in Sacc.). Area freedom had been maintained by restricting the movement of host material and machinery from outside the state and fungicide use in Western Australia vineyards had been considerably less. P. viticola was detected in 1997 in 14 of 15 vines growing at Kalumburu, a remote community in the semi-arid tropics of Western Australia, and was eradicated. In October 1998, grape lea
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B. Santos, Rita, Rui Nascimento, Ana V. Coelho, and Andreia Figueiredo. "Grapevine–Downy Mildew Rendezvous: Proteome Analysis of the First Hours of an Incompatible Interaction." Plants 9, no. 11 (2020): 1498. http://dx.doi.org/10.3390/plants9111498.

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Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. However, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by Plasmopara viticola. Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the P. viticola pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we
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Yu, Ying, Yali Zhang, Ling Yin, and Jiang Lu. "The Mode of Host Resistance to Plasmopara viticola Infection of Grapevines." Phytopathology® 102, no. 11 (2012): 1094–101. http://dx.doi.org/10.1094/phyto-02-12-0028-r.

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The resistance and susceptibility of grapevines to downy mildew (DM) disease caused by Plasmopara viticola were compared among different cultivars/accessions belonging to Vitis vinifera, V. rotundifolia, and 10 oriental Vitis species. After inoculation with P. viticola pathogen, no symptom was found in V. rotundifolia grapevines at all, while oriental species V. davidii and V. piasezkii, like V. vinifera, were susceptible to DM disease. The other eight oriental Vitis species showed various resistance levels to DM disease. Intraspecific resistant variations were also observed in V. amurensis. M
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31

Beffa, Trello. "Metabolism of elemental sulphur (S°) during fungal spore germination." Canadian Journal of Microbiology 39, no. 8 (1993): 736–41. http://dx.doi.org/10.1139/m93-108.

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35S-labelled elemental sulphur (S°) at nontoxic levels (3 μM) was incorporated into sulphur amino acids and glutathione by the spores of the fungus Phomopsis viticola. Incorporation studies were performed with 3 μM 35S° during the pregermination period of P. viticola spores. The free sulphur amino acids and protein sulphur amino acids were purified by column and thin-layer chromatography. During the first minutes of the pregermination process of the spores, the S° was essentially metabolized into free cysteine and glutathione. Detectable concentrations of methionine and homocysteine were measu
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Heyman, Lisa, Antonios Chrysargyris, Kristof Demeestere, Nikolaos Tzortzakis, and Monica Höfte. "Responses to Drought Stress Modulate the Susceptibility to Plasmopara viticola in Vitis vinifera Self-Rooted Cuttings." Plants 10, no. 2 (2021): 273. http://dx.doi.org/10.3390/plants10020273.

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Climate change will increase the occurrence of plants being simultaneously subjected to drought and pathogen stress. Drought can alter the way in which plants respond to pathogens. This research addresses how grapevine responds to the concurrent challenge of drought stress and Plasmopara viticola, the causal agent of downy mildew, and how one stress affects the other. Self-rooted cuttings of the drought-tolerant grapevine cultivar Xynisteri and the drought-sensitive cultivar Chardonnay were exposed to full or deficit irrigation (40% of full irrigation) and artificially inoculated with P. vitic
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Aleksic, Goran, Zoran Milicevic, Slobodan Kuzmanovic, et al. "Efficacy of copper citrate in grapevine disease control." Pesticidi i fitomedicina 34, no. 2 (2019): 103–9. http://dx.doi.org/10.2298/pif1902103a.

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The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0
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34

Baudoin, Anton, Gilberto Olaya, François Delmotte, Jeneylyne F. Colcol, and Helge Sierotzki. "QoI Resistance of Plasmopara viticola and Erysiphe necator in the Mid-Atlantic United States." Plant Health Progress 9, no. 1 (2008): 25. http://dx.doi.org/10.1094/php-2008-0211-02-rs.

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Of 20 Plasmopara viticola isolates collected in four locations in Virginia and northwest North Carolina in 2005, 16 were resistant to QoI fungicides. The resistance factor was over 100, and label rates of formulated azoxystrobin and pyraclostrobin provided little or no control of these isolates. Additional sampling in 2006 revealed at least 15 additional vineyards with QoI-resistant P. viticola in Virginia, Maryland, and Pennsylvania. Of 22 isolates of Erysiphe (Uncinula) necator collected in 2005 from five Virginia locations, 20 isolates from 4 locations showed resistance to QoI fungicides. T
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35

Boso, S., J. L. Santiago, and M. C. Martínez. "Resistance of Eight Different Clones of the Grape Cultivar Albariño to Plasmopara viticola." Plant Disease 88, no. 7 (2004): 741–44. http://dx.doi.org/10.1094/pdis.2004.88.7.741.

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Resistance to downy mildew (Plasmopara viticola) was studied in eight clones of the grape (Vitis vinifera) cultivar Albariño (confirmed as such by DNA and ampelographic analysis) growing at the Misión Biológica de Galicia, Spain. Resistance to downy mildew was quantified using an image processor. Some clones (CSIC-10 and CSIC-11) were more resistant than others to leaf infection by P. viticola. However, the susceptibility of grape clusters did not differ significantly among the clones.
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36

Yi, Tuyong, Shenwei Li, and Yanyun Hong. "Moyeam canker caused by Phomopsis viticola." Journal of Plant Diseases and Protection 126, no. 3 (2019): 261–62. http://dx.doi.org/10.1007/s41348-019-00215-x.

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37

Beffa, Trello. "Inhibitory action of elemental sulphur (S°) on fungal spores." Canadian Journal of Microbiology 39, no. 8 (1993): 731–35. http://dx.doi.org/10.1139/m93-107.

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The fungitoxic effect of increasing concentrations of elemental sulphur (S°) was studied during the pregermination period of spores of Phomopsis viticola and conidia of Neurospora crassa. High concentrations of S° (> 10 μM final concentration) inhibited respiratory activities strongly and decreased the ATP content of spores and conidia. S° at low concentrations (1 and 3 μM final concentration) did not inhibit the respiratory activities or ATP content of spores and conidia. In spores of P. viticola, low concentrations of S° were reduced by the cells with the production of hydrogen sulphide (
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38

Dussert, Yann, Isabelle D. Mazet, Carole Couture, et al. "A High-Quality Grapevine Downy Mildew Genome Assembly Reveals Rapidly Evolving and Lineage-Specific Putative Host Adaptation Genes." Genome Biology and Evolution 11, no. 3 (2019): 954–69. http://dx.doi.org/10.1093/gbe/evz048.

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Abstract Downy mildews are obligate biotrophic oomycete pathogens that cause devastating plant diseases on economically important crops. Plasmopara viticola is the causal agent of grapevine downy mildew, a major disease in vineyards worldwide. We sequenced the genome of Pl. viticola with PacBio long reads and obtained a new 92.94 Mb assembly with high contiguity (359 scaffolds for a N50 of 706.5 kb) due to a better resolution of repeat regions. This assembly presented a high level of gene completeness, recovering 1,592 genes encoding secreted proteins involved in plant–pathogen interactions. P
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39

Nikolic, Dragan. "Components of variance and heritability of resistance to important fungal diseases agents in grapevine." Journal of Agricultural Sciences, Belgrade 51, no. 1 (2006): 47–54. http://dx.doi.org/10.2298/jas0601047n.

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In four interspecies crossing combinations of grapevine (Seedling 108 x Muscat Hamburg, Muscat Hamburg x Seedling 108, S.V.I8315 x Muscat Hamburg and Muscat Hamburg x S.V.I2375) during three years period, resistance to important fungal diseases agents (Plasmopara viticola and Botrytis cinerea) were examined. Based on results of analysis of variance, for investigated characteristics, components of variance, coefficients of genetic and phenotypic variation and coefficient of heritability in a broader sense were calculated. It was established that for both characteristics and in all crossing comb
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40

Gamm, Magdalena, Marie-Claire Héloir, Richard Bligny, et al. "Changes in Carbohydrate Metabolism in Plasmopara viticola-Infected Grapevine Leaves." Molecular Plant-Microbe Interactions® 24, no. 9 (2011): 1061–73. http://dx.doi.org/10.1094/mpmi-02-11-0040.

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The oomycete Plasmopara viticola is responsible for downy mildew, a severe grapevine disease. In infected grapevine leaves, we have observed an abnormal starch accumulation at the end of the dark period, suggesting modifications in starch metabolism. Therefore, several complementary approaches, including transcriptomic analyses, measurements of enzyme activities, and sugar quantification, were performed in order to investigate and to understand the effects of P. viticola infection on leaf starch and—to a larger extent—carbohydrate metabolism. Our results indicate that starch accumulation is as
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41

Erincik, O., L. V. Madden, D. C. Ferree, and M. A. Ellis. "Infection of Grape Berry and Rachis Tissues by Phomopsis viticola." Plant Health Progress 3, no. 1 (2002): 8. http://dx.doi.org/10.1094/php-2002-0702-01-rs.

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Studies were conducted to determine if Phomopsis viticola can infect grape berries directly and cause latent infections, or if fruit infection occurs via mycelial invasion from infected rachis tissues. Examination of inoculated immature berries using scanning electron microscopy showed that alpha spores of P. viticola germinated on the surface of the berry, produced appressoria, and appeared to penetrate the cuticle. Intact clusters of immature berries on ‘Seyval’ grape vines were inoculated with a spore suspension of P. viticola. Clusters were harvested after various periods (12, 18, 24 and 3
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Rekanovic, Emil, Ivana Potocnik, Milos Stepanovic, Svetlana Milijasevic, and Biljana Todorovic. "Field efficacy of fluopicolide and fosetyl-Al fungicide combination (Profiler®) for control of plasmopara viticola (Berk. & Curt.) Berl. & Toni. in grapevine." Pesticidi i fitomedicina 23, no. 3 (2008): 183–87. http://dx.doi.org/10.2298/pif0803183r.

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The efficacy of new fungicide mixtures in controlling Plasmopara viticola in grapevine was evaluated in field trials. The efficacies of Profiler (fluopicolide + fosetyl-Al) and the standard fungicide Mikal Flash (fosetyl-Al + folpet) were tested at Radmilovac and Slankamenacki Vinogradi in 2006 and 2007. Both tested fungicides exhibited high efficacy in controlling grape downy mildew. There were no significant differencies in the efficacies of Profiler (96.1-99.7%) and Mikal Flash (94.9-99.2%). Our experiments showed that the investigated fungicide mixture fluopicolide + fosetyl-Al is highly e
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Kozhevnikov, Evgeniy Anatolievich, Marina Victorovna Makarkina, and Elena Tarasovna Ilnitskaya. "STUDY OF PLASMOPARA VITICOLA POPULATION POLYMORPHISM IN DIFFERENT PATHOGEN GENERATIONS." Fruit growing and viticulture of South Russia 1, no. 67 (2021): 294–304. http://dx.doi.org/10.30679/2219-5335-2021-1-67-294-304.

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44

Peixoto, Ana Rosa, Rosa L. R. Mariano, José Osmã T. Moreira, and Ivanise O. Viana. "Hospedeiros alternativos de Xanthomonas campestris pv. viticola." Fitopatologia Brasileira 32, no. 2 (2007): 161–64. http://dx.doi.org/10.1590/s0100-41582007000200012.

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Xanthomonas campestris pv. viticola (Xcv), que causa o cancro bacteriano da videira, sobrevive em plantas infectadas, epifiticamente em órgãos da parte aérea e pode ser veiculada em mudas e/ou bacelos infectados. O trabalho teve como objetivo investigar possíveis hospedeiros alternativos do patógeno, visando fornecer subsídios para o manejo da doença. A partir das plantas invasoras Alternanthera tenella, Amaranthus sp., Glycine sp. e Senna obtusifolia com sintomas similares aos do cancro bacteriano da videira, coletadas em parreirais de Juazeiro e Petrolina, no Submédio São Francisco, foram is
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45

Sergeeva, V., N. G. Nair, I. Barchia, M. Priest та R. Spooner-Hart. "Germination of β conidia of Phomopsis viticola". Australasian Plant Pathology 32, № 1 (2003): 105. http://dx.doi.org/10.1071/ap02072.

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Allègre, Mathilde, Xavier Daire, Marie-Claire Héloir, et al. "Stomatal deregulation inPlasmopara viticola-infected grapevine leaves." New Phytologist 173, no. 4 (2006): 832–40. http://dx.doi.org/10.1111/j.1469-8137.2006.01959.x.

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47

Toffolatti, Silvia L., Luca Serrati, Helge Sierotzki, Ulrich Gisi, and Annamaria Vercesi. "Assessment of QoI resistance inPlasmopara viticola oospores." Pest Management Science 63, no. 2 (2007): 194–201. http://dx.doi.org/10.1002/ps.1327.

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48

Cossutta, Rada. "I romanismi nella terminologia viticola dell'Istria slovena." Linguistica 35, no. 2 (1995): 3–36. http://dx.doi.org/10.4312/linguistica.35.2.3-36.

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Il presente saggio trae spunto da un attento riesame del materiale che ho raccolto ed analizzato per la mia tesi di dottorato di ricerca dal titolo Poljedelska in vi­ nogradniska terminologija v govorih Slovenske Istre (La terminologia agricola e viticola nelle parlate dell'lstria slovena), tesi che ho discusso presso la Facoltà di Filosofia di Lubiana il 14 gennaio 1994, relatore il prof. Tine Logar, correlatore la prof. Alenka Šivic-Dular. L'opera si inserisce nel filone di ricerche lessicali orientate a ri­ scrivere la storia linguistica di questa regione così complessa per le innumerevoli
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Huang, Xiaoqing, Xina Wang, Fanfang Kong, Theo van der Lee, Zhongyue Wang, and Hao Zhang. "Detection and Characterization of Carboxylic Acid Amide-Resistant Plasmopara viticola in China Using a TaqMan-MGB Real-Time PCR." Plant Disease 104, no. 9 (2020): 2338–45. http://dx.doi.org/10.1094/pdis-02-20-0291-re.

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Grape production is increasing globally and so are problems with downy mildew, one of the main constraints in grape production. Downy mildew on grape is caused by Plasmopara viticola, an obligate biotrophic pathogen belonging to the oomycetes. Control of the disease is usually performed by fungicide applications, of which carboxylic acid amide (CAA) fungicides represent one of the most widely used groups of fungicides. Our previous research showed that the extensive application of CAA fungicides can result in fungicide resistance and in China, CAA-resistant isolates of P. viticola were collect
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Agati, Giovanni, Zoran G. Cerovic, Anna Dalla Marta, et al. "Optically-assessed preformed flavonoids and susceptibility of grapevine to Plasmopara viticola under different light regimes." Functional Plant Biology 35, no. 1 (2008): 77. http://dx.doi.org/10.1071/fp07178.

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The role of flavonoids in the response of plants to Plasmopara viticola, the phytopathogen agent of downy mildew, was studied in the Vitis vinifera L. cultivar Sangiovese. Grapevines in the vineyard were exposed to two light regimes, 100% and 35% of full sunlight in order to induce differences in total leaf polyphenolic content. Epidermal leaf phenolic compounds were assessed optically, using the Dualex chlorophyll fluorescence-based portable leaf-clip. Dualex data were calibrated by means of HPLC analysis of extracts from the same measured leaves. Good correlations were obtained with total fl
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