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Journal articles on the topic 'Voltage-clamp studies'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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1

Claydon, T. W., and D. Fedida. "Voltage clamp fluorimetry studies of mammalian voltage-gated K+ channel gating." Biochemical Society Transactions 35, no. 5 (2007): 1080–82. http://dx.doi.org/10.1042/bst0351080.

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VCF (voltage clamp fluorimetry) provides a powerful technique to observe real-time conformational changes that are associated with ion channel gating. The present review highlights the insights such experiments have provided in understanding Kv (voltage-gated potassium) channel gating, with particular emphasis on the study of mammalian Kv1 channels. Further applications of VCF that would contribute to our understanding of the modulation of Kv channels in health and disease are also discussed.

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2

Lynn, John W., David H. McCulloh, and Edward L. Chambers. "Voltage clamp studies of fertilization in sea urchin eggs." Developmental Biology 128, no. 2 (1988): 305–23. http://dx.doi.org/10.1016/0012-1606(88)90294-1.

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3

Schuster, Bernhard, Dietmar Pum, and Uwe B. Sleytr. "Voltage clamp studies on S-layer-supported tetraether lipid membranes." Biochimica et Biophysica Acta (BBA) - Biomembranes 1369, no. 1 (1998): 51–60. http://dx.doi.org/10.1016/s0005-2736(97)00206-x.

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4

Mleux, Benoit Saint, and L. E. Moore. "Active Dendritic Membrane Properties of XenopusLarval Spinal Neurons Analyzed With a Whole Cell Soma Voltage Clamp." Journal of Neurophysiology 83, no. 3 (2000): 1381–93. http://dx.doi.org/10.1152/jn.2000.83.3.1381.

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Voltage- and current-clamp measurements of inwardly directed currents were made from the somatic regions of Xenopus laevisspinal neurons. Current-voltage ( I-V) curves determined under voltage clamp, but not current clamp, were able to indicate a negative slope conductance in neurons that showed strong accommodating action potential responses to a constant current stimulation. Voltage-clamp I-V curves from repetitive firing neurons did not have a net negative slope conductance and had identical I-V plots under current clamp. Frequency domain responses indicate negative slope conductances with

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5

Díaz-Ríos, Manuel, Daniel A. Dombeck, Watt W. Webb, and Ronald M. Harris-Warrick. "Serotonin Modulates Dendritic Calcium Influx in Commissural Interneurons in the Mouse Spinal Locomotor Network." Journal of Neurophysiology 98, no. 4 (2007): 2157–67. http://dx.doi.org/10.1152/jn.00430.2007.

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Commissural interneurons (CINs) help to coordinate left–right alternating bursting activity during fictive locomotion in the neonatal mouse spinal cord. Serotonin (5-HT) plays an active role in the induction of fictive locomotion in the isolated spinal cord, but the cellular targets and mechanisms of its actions are relatively unknown. We investigated the possible role of serotonin in modifying dendritic calcium currents, using a combination of two-photon microscopy and patch-clamp recordings, in identified CINs in the upper lumbar region. Dendritic calcium responses to applied somatic voltage

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6

Bolton, T. B., R. J. Lang, T. Takewaki, and C. D. Benham. "Patch and Whole-Cell Voltage-Clamp Studies on Single Smooth Muscle Cells." Journal of Cardiovascular Pharmacology 8 (1986): S20—S24. http://dx.doi.org/10.1097/00005344-198600088-00005.

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7

Ye, Q., G. Heck, and J. DeSimone. "The anion paradox in sodium taste reception: resolution by voltage-clamp studies." Science 254, no. 5032 (1991): 724–26. http://dx.doi.org/10.1126/science.1948054.

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8

Klitzner, Thomas, and William F. Friedman. "Excitation-Contraction Coupling in Developing Mammalian Myocardium: Evidence from Voltage Clamp Studies." Pediatric Research 23, no. 4 (1988): 428–32. http://dx.doi.org/10.1203/00006450-198804000-00018.

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9

Sims, S. M., and S. J. Dixon. "Inwardly rectifying K+ current in osteoclasts." American Journal of Physiology-Cell Physiology 256, no. 6 (1989): C1277—C1282. http://dx.doi.org/10.1152/ajpcell.1989.256.6.c1277.

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Membrane properties of freshly isolated rat osteoclasts were studied using the whole cell patch-clamp recording technique. The membrane potential could switch between two stable levels, approximately -70 and -15 mV. Voltage-clamp studies indicated that osteoclasts exhibited marked inward rectification, with hyperpolarizing voltage commands from -70 mV activating large inward currents. No voltage-dependent currents were observed in response to depolarization. An increase in external K+ concentration shifted the current-voltage relationship positive in a manner predicted for K+ current. Furtherm

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10

Zottoli, Steven J. "How the Early Voltage Clamp Studies of José del Castillo Inform “Modern” Neuroscience." Neuroscientist 18, no. 5 (2012): 415–21. http://dx.doi.org/10.1177/1073858412446971.

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11

Birnir, Bryndis, Donald D. F. Loo, and Ernest M. Wright. "Voltage-clamp studies of the Na+/glucose cotransporter cloned from rabbit small intestine." Pfl�gers Archiv European Journal of Physiology 418, no. 1-2 (1991): 79–85. http://dx.doi.org/10.1007/bf00370455.

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12

Maue, R. A., and V. E. Dionne. "Patch-clamp studies of isolated mouse olfactory receptor neurons." Journal of General Physiology 90, no. 1 (1987): 95–125. http://dx.doi.org/10.1085/jgp.90.1.95.

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Olfactory receptor neurons isolated from embryonic, neonatal, and adult mice were studied using the patch-clamp technique. Several distinct types of ion channels were characterized in patches of membrane from the neuronal soma and the dendritic knob of receptor neurons, including a 130-pS Ca++-activated K+ channel with voltage-dependent kinetics, an 80-pS Ca++-activated K+ channel with voltage-insensitive kinetics, a 25-pS K+ channel with properties similar to inward rectifiers, and a 40-pS K+ channel that was activated and then inactivated by rapid depolarization. Evidence of large-conductanc

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13

Imoto, Y., T. Ehara, and H. Matsuura. "Voltage- and time-dependent block of iK1 underlying Ba2+-induced ventricular automaticity." American Journal of Physiology-Heart and Circulatory Physiology 252, no. 2 (1987): H325—H333. http://dx.doi.org/10.1152/ajpheart.1987.252.2.h325.

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The mechanism underlying the Ba2+-induced automaticity was studied in isolated guinea pig ventricular myocytes using the whole-cell clamp method and a patch electrode. In the presence of 0.1–0.3 mM Ba2+, application of a weak depolarizing current induced repetitive firing of spontaneous action potentials. Application of tetrodotoxin or Ca2+ channel blockers and removal of external Na+ and Ca2+ did not abolish the rhythmic activity, thereby suggesting that activation of inward currents played no crucial role in the generation of this rhythmicity. Voltage-clamp studies revealed that Ba2+ blocked

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14

Cho, Hyun-jung, John B. Furness, and Ernest A. Jennings. "Postnatal maturation of the hyperpolarization-activated cation current, Ih, in trigeminal sensory neurons." Journal of Neurophysiology 106, no. 4 (2011): 2045–56. http://dx.doi.org/10.1152/jn.00798.2010.

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Hyperpolarization-activated inward currents ( Ih) contribute to neuronal excitability in sensory neurons. Four subtypes of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels generate Ih, with different activation kinetics and cAMP sensitivities. The aim of the present study was to examine the postnatal development of Ih and HCN channel subunits in trigeminal ganglion (TG) neurons. Ih was investigated in acutely dissociated TG neurons from rats aged between postnatal day (P)1 and P35 with whole cell patch-clamp electrophysiology. In voltage-clamp studies, Ih was activated by a s

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15

Akk, Gustav, та Joe Henry Steinbach. "Structural Studies of the Actions of Anesthetic Drugs on the γ-Aminobutyric Acid Type A Receptor". Anesthesiology 115, № 6 (2011): 1338–48. http://dx.doi.org/10.1097/aln.0b013e3182315d93.

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The γ-aminobutyric acid type A receptor is the major transmitter-gated inhibitory channel in the central nervous system. The receptor is a target for anesthetics, anticonvulsants, anxiolytics, and sedatives whose actions facilitate the flow of chloride ions through the channel and enhance the inhibitory tone in the brain. Both the kinetic and structural aspects of the actions of modulators of the γ-aminobutyric acid type A receptor are of great importance to understanding the molecular mechanisms of general anesthesia. In this review, the structural rearrangements that take place in the γ-amin

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16

Frolov, Roman V. "Current advances in invertebrate vision: insights from patch-clamp studies of photoreceptors in apposition eyes." Journal of Neurophysiology 116, no. 2 (2016): 709–23. http://dx.doi.org/10.1152/jn.00288.2016.

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Traditional electrophysiological research on invertebrate photoreceptors has been conducted in vivo, using intracellular recordings from intact compound eyes. The only exception used to be Drosophila melanogaster, which was exhaustively studied by both intracellular recording and patch-clamp methods. Recently, several patch-clamp studies have provided new information on the biophysical properties of photoreceptors of diverse insect species, having both apposition and neural superposition eyes, in the contexts of visual ecology, behavior, and ontogenesis. Here, I discuss these and other relevan

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17

Berman, Jonathan M., and Mouhamed S. Awayda. "Redox artifacts in electrophysiological recordings." American Journal of Physiology-Cell Physiology 304, no. 7 (2013): C604—C613. http://dx.doi.org/10.1152/ajpcell.00318.2012.

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Electrophysiological techniques make use of Ag/AgCl electrodes that are in direct contact with cells or bath. In the bath, electrodes are exposed to numerous experimental conditions and chemical reagents that can modify electrode voltage. We examined voltage offsets created in Ag/AgCl electrodes by exposure to redox reagents used in electrophysiological studies. Voltage offsets were measured in reference to an electrode separated from the solution by an agar bridge. The reducing reagents Tris-2-carboxyethly-phosphine, dithiothreitol (DTT), and glutathione, as well as the oxidizing agent H2O2us

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18

Torkkeli, Päivi H., and Andrew S. French. "Simulation of Different Firing Patterns in Paired Spider Mechanoreceptor Neurons: The Role of Na+ Channel Inactivation." Journal of Neurophysiology 87, no. 3 (2002): 1363–68. http://dx.doi.org/10.1152/jn.00440.2001.

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The spider VS-3 slit-sense organ contains two types of primary mechanoreceptor neurons that are morphologically similar but have different electrical behavior. Type A neurons fire only one or two action potentials in response to a mechanical or electrical step of any amplitude above the threshold, whereas type B neurons fire prolonged bursts of action potentials in response to similar stimuli. Voltage-clamp studies have shown that two voltage-activated ion currents, a noninactivating potassium current and an inactivating sodium current, dominate the firing behavior. We simulated the electrical

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19

Rae, J. L., and M. A. Watsky. "Ionic channels in corneal endothelium." American Journal of Physiology-Cell Physiology 270, no. 4 (1996): C975—C989. http://dx.doi.org/10.1152/ajpcell.1996.270.4.c975.

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Single-channel patch-clamp techniques as well as standard and perforated-patch whole cell voltage-clamp techniques have been applied to the study of ionic channels in the corneal endothelium of several species. These studies have revealed two major K+ currents. One is due to an anion- and temperature-stimulated channel that is blocked by Cs+ but not by most other K+ channel blockers, and the other is similar to the family of A-currents found in excitable cells. The A-current is transient after a depolarizing voltage step and is blocked by both 4-aminopyridine and quinidine. These two currents

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20

Cordeiro, Jonathan M., Lindsey Greene, Cory Heilmann, Daniel Antzelevitch, and Charles Antzelevitch. "Transmural heterogeneity of calcium activity and mechanical function in the canine left ventricle." American Journal of Physiology-Heart and Circulatory Physiology 286, no. 4 (2004): H1471—H1479. http://dx.doi.org/10.1152/ajpheart.00748.2003.

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Although electrical heterogeneity within the ventricular myocardium has been the focus of numerous studies, little attention has been directed to the mechanical correlates. This study examines unloaded cell shortening, Ca2+ transients, and inward L-type Ca2+ current ( ICa,L) characteristics of epicardial, endocardial, and midmyocardial cells isolated from the canine left ventricle. Unloaded cell shortening was recorded using a video edge detector, Ca2+ transients were measured in cells loaded with 15 μM fluo-3 AM and voltage and current-clamp recordings were obtained using patch-clamp techniqu

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21

Liu, S., and A. J. Mautone. "Whole cell potassium currents in fetal rat alveolar type II cells cultured on Matrigel matrix." American Journal of Physiology-Lung Cellular and Molecular Physiology 270, no. 4 (1996): L577—L586. http://dx.doi.org/10.1152/ajplung.1996.270.4.l577.

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Patch-clamp studies were performed on fetal rat alveolar type II cells isolated at 19 days of gestation and cultured on either plastic for 7 days or Matrigel matrix (40-50 microliters/cm2) for 10 days. Before study, cells cultured on Matrigel matrix were dissociated from alveolar-like structures with enzymes, replated, and washed with cold buffer at a constant flow rate to remove residual gel. This wash significantly improved obtaining of successful seals. Potassium current-voltage relationships and maximum whole cell K+ conductance (normalized to membrane capacitance) were significantly chang

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22

Powers, Randall K., and Marc D. Binder. "Persistent Sodium and Calcium Currents in Rat Hypoglossal Motoneurons." Journal of Neurophysiology 89, no. 1 (2003): 615–24. http://dx.doi.org/10.1152/jn.00241.2002.

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Voltage-dependent persistent inward currents are thought to make an important contribution to the input–output properties of α−motoneurons, influencing both the transfer of synaptic current to the soma and the effects of that current on repetitive discharge. Recent studies have paid particular attention to the contribution of L-type calcium channels, which are thought to be widely distributed on both the somatic and the dendritic membrane. However, the relative contribution of different channel subtypes as well as their somatodendritic distribution may vary among motoneurons of different speci

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23

Yadav, Vivek, Nicholas Chong, Bradley Ellis, et al. "Constant-potential environment for activating and synchronizing cardiomyocyte colonies with on-chip ion-depleting perm-selective membranes." Lab on a Chip 20, no. 22 (2020): 4273–84. http://dx.doi.org/10.1039/d0lc00809e.

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An ion depleted zone was used to impose a high and uniform constant extracellular potential over an entire ∼1000 cell rat cardiomyocyte (rCM) colony on-a-chip, extending single-cell voltage-clamp ion channel studies to an entire normalized colony.

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24

Hollins, B., and S. R. Ikeda. "Inward currents underlying action potentials in rat adrenal chromaffin cells." Journal of Neurophysiology 76, no. 2 (1996): 1195–211. http://dx.doi.org/10.1152/jn.1996.76.2.1195.

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1. Current- and voltage-clamp studies were conducted on isolated rat adrenal chromaffin cells to identify the voltage-dependent ion channels mediating inward currents. 2. Mean resting membrane potential of the isolated cells was -62 +/- 3 (SE) mV. Evoked action potentials were both Na+ and Ca2+ based, and whole cell voltage-clamp studies in normal saline revealed an inward-rectifier-type current. 3. Na+ channels were studied in isolation and showed a half-inactivation of -60 +/- 2 mV with a slope factor of -6 mV and a half-activation of -26.8 +/- 2 mV with a slope factor of 6.5 +/- 0.7 mV. 4.

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25

Miyoshi, H., M. B. Boyle, L. B. MacKay, and R. E. Garfield. "Voltage-clamp studies of gap junctions between uterine muscle cells during term and preterm labor." Biophysical Journal 71, no. 3 (1996): 1324–34. http://dx.doi.org/10.1016/s0006-3495(96)79332-3.

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26

Hamill, O. P., J. R. Huguenard, and D. A. Prince. "Patch-Clamp Studies of Voltage-Gated Currents in Identified Neurons of the Rat Cerebral Cortex." Cerebral Cortex 1, no. 1 (1991): 48–61. http://dx.doi.org/10.1093/cercor/1.1.48.

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27

Velte, T. J., and R. F. Miller. "Computer simulations of voltage clamping retinal ganglion cells through whole-cell electrodes in the soma." Journal of Neurophysiology 75, no. 5 (1996): 2129–43. http://dx.doi.org/10.1152/jn.1996.75.5.2129.

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1. Computer simulations of voltage-clamp experiments in retinal ganglion cells were implemented to better understand the insights that can be obtained with this physiological approach. 2. Simulation studies of voltage clamping were based on the contemporary approach of using whole-cell recordings with low resistance electrodes attached to the soma. Realistic ganglion cell morphologies were provided by cell staining experiments in the mudpuppy retina; selected cells included small-, medium-, and large-field neurons whose morphologies were entered into a computer through a neuron tracing program

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28

Niggli, E., A. Rudisuli, P. Maurer, and R. Weingart. "Effects of general anesthetics on current flow across membranes in guinea pig myocytes." American Journal of Physiology-Cell Physiology 256, no. 2 (1989): C273—C281. http://dx.doi.org/10.1152/ajpcell.1989.256.2.c273.

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Myocytes were isolated from adult guinea pig ventricles. Whole cell, tight-seal recording was employed to investigate the electrical properties of the junctional (nexal membrane) and nonjunctional membrane (sarcolemma) under the influence of n-alkanols (heptanol, octanol) and halothane. Studies of cell pairs with a double voltage-clamp approach showed that these agents give rise to a reversible electrical uncoupling. Examination of single myocytes with a single voltage-clamp method showed that these substances modify several sarcolemmal current systems. The slope conductance was reduced over t

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29

Buhry, Laure, Filippo Grassia, Audrey Giremus, Eric Grivel, Sylvie Renaud, and Sylvain Saïghi. "Automated Parameter Estimation of the Hodgkin-Huxley Model Using the Differential Evolution Algorithm: Application to Neuromimetic Analog Integrated Circuits." Neural Computation 23, no. 10 (2011): 2599–625. http://dx.doi.org/10.1162/neco_a_00170.

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We propose a new estimation method for the characterization of the Hodgkin-Huxley formalism. This method is an alternative technique to the classical estimation methods associated with voltage clamp measurements. It uses voltage clamp type recordings, but is based on the differential evolution algorithm. The parameters of an ionic channel are estimated simultaneously, such that the usual approximations of classical methods are avoided and all the parameters of the model, including the time constant, can be correctly optimized. In a second step, this new estimation technique is applied to the a

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30

Correia, M. J., B. N. Christensen, L. E. Moore, and D. G. Lang. "Studies of solitary semicircular canal hair cells in the adult pigeon. I. Frequency- and time-domain analysis of active and passive membrane properties." Journal of Neurophysiology 62, no. 4 (1989): 924–34. http://dx.doi.org/10.1152/jn.1989.62.4.924.

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1. Hair cells were enzymatically dissociated from the neuroepithelium (cristae ampullares) of the semicircular canals of white king pigeons (Columba livia). Those hair cells determined to be type II by an anatomic criterion, the ratio of the minimum width of the neck to the width of the cuticular plate, were studied with the use of the whole cell patch-clamp technique. 2. The mean +/- SD zero-current membrane potential, Vz, was found to be -54 +/- 12 mV for anterior crista hair cells (n = 71), -62 +/- 14 mV for posterior crista hair cells (n = 14), and -55 +/- 12 mV for lateral (horizontal) cr

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31

Smith, R. Ya, D. Morgan, L. Sharma, et al. "Voltage-gated proton channels exist in the plasma membrane of human oocytes." Human Reproduction 34, no. 10 (2019): 1974–83. http://dx.doi.org/10.1093/humrep/dez178.

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Abstract STUDY QUESTION Do human oocytes express voltage-gated proton channels? SUMMARY ANSWER Human oocytes exhibit voltage-gated proton currents. WHAT IS KNOWN ALREADY Voltage-gated proton currents have been reported in human sperm, where they contribute to capacitation and motility. No such studies of human oocytes exist. STUDY DESIGN, SIZE, DURATION Voltage-clamp studies were undertaken using entire oocytes and vesicles derived from oocytes and in excised patches of membrane from oocytes. PARTICIPANTS/MATERIALS, SETTING, METHODS Frozen, thawed human metaphase II oocytes were obtained from

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32

Berestovsky, Genrikh N., and Anatoly A. Kataev. "Voltage-gated calcium and Ca2+-activated chloride channels and Ca2+ transients: voltage-clamp studies of perfused and intact cells of Chara." European Biophysics Journal 34, no. 8 (2005): 973–86. http://dx.doi.org/10.1007/s00249-005-0477-9.

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Evans, Michael G., Arwa Al-Shakli, and Divya M. Chari. "Electrophysiological properties of neurons grown on soft polymer scaffolds reveal the potential to develop neuromimetic culture environments." Integrative Biology 11, no. 11 (2019): 395–403. http://dx.doi.org/10.1093/intbio/zyz033.

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Abstract Tissue engineering methodologies for various physiological systems are seeing a significant trend towards 3D cell culture in or on ‘soft’ polymeric hydrogel materials, widely considered to provide a more biomimetic environment for cell growth versus ‘hard’ materials such as glass or plastic. Progress has been slower with 3D neural cell culture with current studies overwhelmingly reliant on hard substrates. Accordingly, our knowledge of the alterations in electrochemical properties of neurons propagated in soft materials is relatively limited. In this study, primary cortical neurons an

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de Jeu, Marcel, Alwin Geurtsen, and Cyriel Pennartz. "A Ba2+-Sensitive K+ Current Contributes to the Resting Membrane Potential of Neurons in Rat Suprachiasmatic Nucleus." Journal of Neurophysiology 88, no. 2 (2002): 869–78. http://dx.doi.org/10.1152/jn.2002.88.2.869.

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A Ba2+-sensitive K+ current was studied in neurons of the suprachiasmatic nucleus (SCN) using the whole cell patch-clamp technique in acutely prepared brain slices. This Ba2+-sensitive K+ current was found in approximately 90% of the SCN neurons and was uniformly distributed across the SCN. Current-clamp studies revealed that Ba2+ (500 μM) reversibly depolarized the membrane potential by 6.7 ± 1.3 mV ( n = 22) and concomitantly Ba2+ induced an increase in the spontaneous firing rate of 0.8 ± 0.2 Hz ( n = 12). The Ba2+-evoked depolarizations did not depend on firing activity or spike dependent

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Won, Yu-Jin, Fumihito Ono, and Stephen R. Ikeda. "Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons." Journal of Neurophysiology 105, no. 1 (2011): 442–53. http://dx.doi.org/10.1152/jn.00625.2010.

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Electrically excitable cells have voltage-dependent ion channels on the plasma membrane that regulate membrane permeability to specific ions. Voltage-gated Ca2+ channels (VGCCs) are especially important as Ca2+ serves as both a charge carrier and second messenger. Zebrafish ( Danio rerio) are an important model vertebrate for studies of neuronal excitability, circuits, and behavior. However, electrophysiological properties of zebrafish VGCCs remain largely unexplored because a suitable preparation for whole cell voltage-clamp studies is lacking. Rohon-Beard (R-B) sensory neurons represent an a

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Wu, Fenfen, Marbella Quinonez, Marino DiFranco, and Stephen C. Cannon. "Stac3 enhances expression of human CaV1.1 in Xenopus oocytes and reveals gating pore currents in HypoPP mutant channels." Journal of General Physiology 150, no. 3 (2018): 475–89. http://dx.doi.org/10.1085/jgp.201711962.

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Mutations of CaV1.1, the pore-forming subunit of the L-type Ca2+ channel in skeletal muscle, are an established cause of hypokalemic periodic paralysis (HypoPP). However, functional assessment of HypoPP mutant channels has been hampered by difficulties in achieving sufficient plasma membrane expression in cells that are not of muscle origin. In this study, we show that coexpression of Stac3 dramatically increases the expression of human CaV1.1 (plus α2-δ1b and β1a subunits) at the plasma membrane of Xenopus laevis oocytes. In voltage-clamp studies with the cut-open oocyte clamp, we observe ion

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Baxter, Deborah F., Martin Kirk, Amy F. Garcia, et al. "A Novel Membrane Potential-Sensitive Fluorescent Dye Improves Cell-Based Assays for Ion Channels." Journal of Biomolecular Screening 7, no. 1 (2002): 79–85. http://dx.doi.org/10.1177/108705710200700110.

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The study of ion channel-mediated changes in membrane potential using the conventional bisoxonol fluorescent dye DiBAC4(3) has several limitations, including a slow onset of response and multistep preparation, that limit both the fidelity of the results and the throughput of membrane potential assays. Here, we report the characterization of the FLIPR Membrane Potential Assay Kit (FMP) in cells expressing voltage- and ligand-gated ion channels. The steady-state and kinetics fluorescence properties of FMP were compared with those of DiBAC4(3), using both FLIPR and whole-cell patch-clamp recordin

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Grolleau, F., and B. Lapied. "Dorsal unpaired median neurones in the insect central nervous system: towards a better understanding of the ionic mechanisms underlying spontaneous electrical activity." Journal of Experimental Biology 203, no. 11 (2000): 1633–48. http://dx.doi.org/10.1242/jeb.203.11.1633.

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The efferent dorsal unpaired median (DUM) neurones, which include octopaminergic neurones, are among the most intensively studied neurones in the insect central nervous system. They differ from other insect neurones in generating endogenous spontaneous overshooting action potentials. The second half of the 1980s is certain to be considered a turning point in the study of the ion channels underlying the electrical activity of DUM neurones. Recent advances made using the patch-clamp technique have stimulated an increasing interest in the understanding of the biophysical properties of both voltag

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Rabbah, Pascale, Jorge Golowasch, and Farzan Nadim. "Effect of Electrical Coupling on Ionic Current and Synaptic Potential Measurements." Journal of Neurophysiology 94, no. 1 (2005): 519–30. http://dx.doi.org/10.1152/jn.00043.2005.

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Recent studies have found electrical coupling to be more ubiquitous than previously thought, and coupling through gap junctions is known to play a crucial role in neuronal function and network output. In particular, current spread through gap junctions may affect the activation of voltage-dependent conductances as well as chemical synaptic release. Using voltage-clamp recordings of two strongly electrically coupled neurons of the lobster stomatogastric ganglion and conductance-based models of these neurons, we identified effects of electrical coupling on the measurement of leak and voltage-gat

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Toh, May Fern, Julie M. Brooks, Tim Strassmaier, et al. "Application of High-Throughput Automated Patch-Clamp Electrophysiology to Study Voltage-Gated Ion Channel Function in Primary Cortical Cultures." SLAS DISCOVERY: Advancing the Science of Drug Discovery 25, no. 5 (2020): 447–57. http://dx.doi.org/10.1177/2472555220902388.

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Conventionally, manual patch-clamp electrophysiological approaches are the gold standard for studying ion channel function in neurons. However, these approaches are labor-intensive, yielding low-throughput results, and are therefore not amenable for compound profiling efforts during the early stages of drug discovery. The SyncroPatch 384PE has been successfully implemented for pharmacological experiments in heterologous overexpression systems that may not reproduce the function of voltage-gated ion channels in a native, heterogeneous environment. Here, we describe a protocol allowing the chara

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Rose, R. A., A. E. Lomax, and W. R. Giles. "Inhibition of L-type Ca2+ current by C-type natriuretic peptide in bullfrog atrial myocytes: an NPR-C-mediated effect." American Journal of Physiology-Heart and Circulatory Physiology 285, no. 6 (2003): H2454—H2462. http://dx.doi.org/10.1152/ajpheart.00388.2003.

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Single atrial myocytes were isolated from the bullfrog heart and studied under current and voltage clamp conditions to determine the electrophysiological effects of the C-type natriuretic peptide (CNP). CNP (10–8 M) significantly shortened the action potential and reduced its peak amplitude after the application of isoproteronol (10–7 M). In voltage clamp studies, CNP inhibited isoproteronol-stimulated L-type Ca2+ current ( ICa) without any significant effect on the inward rectifier K+ current. The effects of cANF (10–8 M), a selective agonist of the natriuretic peptide C receptor (NPR-C), wer

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Weng, Tianxiang, and Manning J. Correia. "Regional Distribution of Ionic Currents and Membrane Voltage Responses of Type II Hair Cells in the Vestibular Neuroepithelium." Journal of Neurophysiology 82, no. 5 (1999): 2451–61. http://dx.doi.org/10.1152/jn.1999.82.5.2451.

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Basolateral ionic currents and membrane voltage responses were studied in pigeon vestibular type II hair cells using a thin slice through either the semicircular canal (SCC) crista or utricular macular epithelium. Whole cell tight-seal patch-clamp recording techniques were used. Current-clamp and voltage-clamp studies were carried out on the same cell. One hundred ten cells were studied in the peripheral (Zone I) and central (Zone III) zones of the SCC crista, and 162 cells were studied in the striolar (S Zone) and extrastriolar (ES Zone) zones of the utricular macula. One of the major finding

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Toro, L., A. Gonzalez-Robles, and E. Stefani. "Electrical properties and morphology of single vascular smooth muscle cells in culture." American Journal of Physiology-Cell Physiology 251, no. 5 (1986): C763—C773. http://dx.doi.org/10.1152/ajpcell.1986.251.5.c763.

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Single vascular smooth muscle cells (VSMC) were isolated from the caudal artery and vein and studied after 2 or 3 days in culture. Current clamp with intracellular microelectrodes and "whole-cell" voltage-clamp techniques were used. Also, scanning and transmission electron microscopy studies were performed, revealing morphological characteristics of smooth muscle in culture. Cells could contract in response to electrical and chemical stimuli. The passive membrane properties recorded with intracellular microelectrodes in a mammalian saline were as follows: 1) for artery, resting potential Vm =

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Menestrina, Gianfranco, and Flavia Pasquali. "Effects of Carbohydrates on the Ion Conductance of the Hemocyanin Channel." Zeitschrift für Naturforschung C 40, no. 1-2 (1985): 85–91. http://dx.doi.org/10.1515/znc-1985-1-217.

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Abstract The effects of glucose and sucrose on the ionic conductance properties of the channel formed by Megathura crenulata hemocyanin in planar lipid bilayers have been studied using membranes of different compositions. It was found that glucose at high concentrations strongly affects the time constants of the current relaxations observed in membranes containing many channels after a step in the voltage clamp from ground to a positive value. At much lower concentrations both sucrose and glucose strengthened the binding of Ba2+ to the protein, what in turn has the effect to shift the conducta

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Hasan, Md Mahadhi, Lotten Ragnarsson, Fernanda C. Cardoso, and Richard J. Lewis. "Transfection methods for high-throughput cellular assays of voltage-gated calcium and sodium channels involved in pain." PLOS ONE 16, no. 3 (2021): e0243645. http://dx.doi.org/10.1371/journal.pone.0243645.

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Chemical transfection is broadly used to transiently transfect mammalian cells, although often associated with cellular stress and membrane instability, which imposes challenges for most cellular assays, including high-throughput (HT) assays. In the current study, we compared the effectiveness of calcium phosphate, FuGENE and Lipofectamine 3000 to transiently express two key voltage-gated ion channels critical in pain pathways, CaV2.2 and NaV1.7. The expression and function of these channels were validated using two HT platforms, the Fluorescence Imaging Plate Reader FLIPRTetra and the automat

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D’hoedt, Dieter, Victor Tsetlin, Sonia Bertrand, and Daniel Bertrand. "Automated two-electrode voltage clamp for medium-throughput studies of ion channels with non-destructive sample analysis." Biochemical Pharmacology 78, no. 7 (2009): 907. http://dx.doi.org/10.1016/j.bcp.2009.06.048.

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Imon, K., T. Amano, K. Ishihara, M. Sasa, and K. Yajin. "Existence of voltage-dependent Ca2+ channels in vestibular dark cells: cytochemical and whole-cell patch-clamp studies." European Archives of Oto-Rhino-Laryngology 254, no. 6 (1997): 287–91. http://dx.doi.org/10.1007/bf02905990.

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Shi, Hong, Hui-Zhen Wang, and Zhiguo Wang. "Extracellular Ba2+ blocks the cardiac transient outward K+ current." American Journal of Physiology-Heart and Circulatory Physiology 278, no. 1 (2000): H295—H299. http://dx.doi.org/10.1152/ajpheart.2000.278.1.h295.

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Ba2+ is widely used as a tool in patch-clamp studies because of its ability to block a variety of K+channels and to pass Ca2+ channels. Its potential ability to block the cardiac transient outward K+ current ( I to) has not been clearly documented. We performed whole cell patch-clamp studies in canine ventricular and atrial myocytes. Extracellular application of Ba2+ produced potent inhibition of I to with an IC50 of ∼40 μM. The effects were voltage independent, and the inactivation kinetics were not altered by Ba2+. The potency of Ba2+ was ∼10 times higher than that of 4-aminopyridine (a sele

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Vacher, P., J. McKenzie, and B. Dufy. "Arachidonic acid affects membrane ionic conductances of GH3 pituitary cells." American Journal of Physiology-Endocrinology and Metabolism 257, no. 2 (1989): E203—E211. http://dx.doi.org/10.1152/ajpendo.1989.257.2.e203.

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Arachidonic acid (AA) stimulates prolactin release from pituitary cells, by mechanisms not yet understood. In this work, we analyzed the effects of AA on membrane ionic conductances in a clonal line of anterior pituitary cells (GH3/B6), finding time- and dose-dependent effects of AA on their membrane ionic conductances. The predominant response at concentrations between 100 nM and 10 microM was a prolongation of the action potential (AP) and an increase in the transient after-hyperpolarization potential. Voltage clamp studies showed that this was associated with a decrease in a voltage-depende

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Hristov, Kiril L., Amy C. Smith, Shankar P. Parajuli, John Malysz, and Georgi V. Petkov. "Large-conductance voltage- and Ca2+-activated K+ channel regulation by protein kinase C in guinea pig urinary bladder smooth muscle." American Journal of Physiology-Cell Physiology 306, no. 5 (2014): C460—C470. http://dx.doi.org/10.1152/ajpcell.00325.2013.

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Large-conductance voltage- and Ca2+-activated K+ (BK) channels are critical regulators of detrusor smooth muscle (DSM) excitability and contractility. PKC modulates the contraction of DSM and BK channel activity in non-DSM cells; however, the cellular mechanism regulating the PKC-BK channel interaction in DSM remains unknown. We provide a novel mechanistic insight into BK channel regulation by PKC in DSM. We used patch-clamp electrophysiology, live-cell Ca2+ imaging, and functional studies of DSM contractility to elucidate BK channel regulation by PKC at cellular and tissue levels. Voltage-cla

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