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Journal articles on the topic 'Volume and culture'

Author: Grafiati

Published: 4 June 2025

Last updated: 23 June 2025

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1

Libertin, Claudia R., Keith A. Sacco, and Joy H. Peterson. "Education and coaching to optimise blood culture volumes: continuous quality improvement in microbiology." BMJ Open Quality 7, no. 3 (2018): e000228. http://dx.doi.org/10.1136/bmjoq-2017-000228.

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The blood volume cultured in the detection of bacteraemia is a major variable in treating patients with systemic inflammatory response syndrome. The fact that drawing optimal volumes (8–10 mL) of blood for culture increases the sensitivity of the method is well established. This study aimed to optimise the mean blood volumes (mBVs) to that recommended level in a small rural hospital by implementing a continuous quality improvement programme in clinical microbiology. The education of phlebotomists, followed by monthly feedback and coaching sessions, can influence the blood volume drawn by phlebotomists and improve the sensitivity of blood cultures. Statistically significant increase (p<0.001) in both mBVs and median blood culture volumes occurred within 5 months compared with the baseline values obtained in the preceding 10 months. This quality improvement was sustained over 1 year. The mBVs inoculated into aerobic culture bottles met the manufacturer’s instructions of a fill volume of 8 to 10 mL of blood per bottle and optimised the yield of isolation of organisms from blood cultures.

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2

Mulyani, Laily Fitriani, Nuri Muahiddah, and Thoy Batun Citra Rahmadani. "GROWTH PHASE OF ISOCHRYSIS GALBANA NATURAL FEED WITH DIFFERENT CULTURE MEDIA VOLUMES ON LABORATORY SCALE." Jurnal Perikanan Unram 14, no. 4 (2025): 2365–73. https://doi.org/10.29303/jp.v14i4.1286.

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One of the causes of low growth and survival in the early development of larvae that still rely on food from natural feed is the availability of feed. The purpose of this study was to analyze the percentage level of increase in the density of the amount of natural feed cultured in Erlenmeyer media with different volumes of culture media. Completely Randomized Design with 3 treatments and 5 replications. The treatments used were 500 ml, 1000 ml and 5000 ml culture media volumes. The results of the study showed that the 500 ml volume culture media produced a density increase of 20%, then for the 1000 ml volume culture media it produced a density increase of 16% and for the 5000 ml volume culture media it produced an increase in density of 8%. So that with a 500 ml volume culture media it can effectively increase the density of I. galbana.

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3

Altez, Maria S. Rueda, Lamia Soghier, Joseph M. Campos, James Bost, Jiaxiang Gai, and Rana F. Hamdy. "1341. Blood Volume Collected for Blood Cultures in Infants with Suspected Neonatal Sepsis in the NICU." Open Forum Infectious Diseases 7, Supplement_1 (2020): S682. http://dx.doi.org/10.1093/ofid/ofaa439.1523.

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Abstract Background Blood cultures have high sensitivity to detect bacteremia in septic neonates when &gt;=1 ml of blood is collected. Neonatologists often cite low confidence in microbiologic sampling as rationale for continuing antibiotics without a focus of infection despite negative blood cultures, resulting in prolonged antimicrobial therapy. We aim to describe the blood culture sample volumes in NICU patients, to identify factors associated with sample volumes &lt; 1ml, and to compare the sample volumes of patients treated for culture-negative sepsis with those with bloodstream infections and those treated for a ≤72-hour sepsis rule-out Methods Data from this observational cohort study were collected retrospectively and prospectively from NICU patients with blood cultures obtained from September 2018 to February 2019. Clinical data were collected through chart review. All inoculated culture bottles were weighed for volume calculation. We determined the association of age, weight, sample source, and time of collection with volume &lt; 1mL. Continuous variables were analyzed using Wilcoxon-Mann-Whitney, and categorical variables using chi-squared test. For aim 3, the volumes of the groups were compared using analysis of variance. Results A total of 310 blood cultures were identified, corresponding to 159 patients. Of these, 49 (16%) were positive. Among the negative blood cultures, 86% were collected in patients who subsequently received antibiotics (Figure 1). Median inoculated volume was 0.6 ml (IQR: 0.1-2.4). Weight and age at time of culture collection, source of sample, and time of collection were not significantly associated with the inoculation of &lt; 1ml of blood. Median volume of blood was 0.6ml (0.3-0.6) for sepsis rule-out, 0.6ml (0.2-0.6) for bloodstream infection, and 0.6ml (0.6-1.4) for culture-negative sepsis. No difference was found among the three groups (p=0.54) Figure 1. Classification of blood cultures identified during study period Conclusion The blood volume collected for cultures in the NICU is lower than recommended. Clinical and environmental characteristics are not significantly associated with the inoculated volume. The volume of blood sampled does not differ in patients with culture-negative sepsis, bloodstream infection and sepsis rule-out, and should not be a justification for longer duration of antibiotic therapy Disclosures All Authors: No reported disclosures

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4

Tisserat, Brent, Robert Silman, and Karen Ray. "Interaction of Culture Vessel Size, Medium Volume, and Carbon Dioxide Levels on the Growth of Various Plants in Vitro." HortScience 32, no. 3 (1997): 515D—515. http://dx.doi.org/10.21273/hortsci.32.3.515d.

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Ultra-high levels of CO2, i.e., >10,000 ppm, enhance tissue culture growth and offers a relatively simple and inexpensive method to improve plant productivity in vitro. Growth responses employing ultra-high CO2 levels differ considerably in the literature. Unfortunately, various culture vessels and systems have been employed, making comparisons difficult. In this study, the influence of the vessel container size, medium volume, and various CO2 concentrations (0 to 50,000 ppm) was studied on the growth obtained from lettuce and spearmint cultures. All three of these factors influence growth responses from plants cultured in vitro. Vessel types tested included: culture tubes, Magenta containers, 1-quart jars, 0.5-gallon jars, and 1-gallon jars having culture volumes of 55, 365, 925, 1850, and 3700 ml, respectively. Increasing the size of the culture vessel resulted in an increase growth regardless of the CO2 level tested. For example, fresh weight of spearmint increases of >250% can be obtained in by employing a 1-quart jar compared to using a culture tube. Increasing medium volume using various vessel types, especially using high concentrations of CO2, resulted in dramatic growth increases. For example, a >100% increase in fresh weight could be obtained by increasing the medium volume from 50 ml to 100 ml within a 1-quart jar. These studies suggest that plant growth promoted by supplemental CO2 is limited by the culture vessel size and medium volume. Differences in growth responses obtained in past CO2 studies could be related to vessel type and medium volume as well as the CO2 levels employed. Future in vitro studies should consider these factors in the evaluation of the influence of Ultra-high CO2 levels on plant growth. Peculiar growth responses, especially pertaining to rooting and shooting exhibited by cultures grown in ultra-high CO2 levels will also be discussed.

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5

Touché, Marc, Gérome Guibert, and Fabien Hein. "Metal. Une culture de la transgression sonore." Volume !, no. 5 : 2 (September 15, 2006): 137–52. http://dx.doi.org/10.4000/volume.539.

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6

Andersen, Lis, and Dorthe Arenholt-Bindslev. "Morphometric Registration of Cytotoxic Changes of Epithelial Cells In Vitro: A Methodological Study." Alternatives to Laboratory Animals 17, no. 3 (1990): 174–76. http://dx.doi.org/10.1177/026119299001700307.

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Quantification of toxicity-induced cytomorphological effects in an epithelial cell culture system is described. Estimates of volume density and star volume of mitochondria and lysosomes are given. Mean volumes (n = 5) and coefficients of variation of these parameters were equal in experimental (TPA-treatment) and control cultures. An optimal allocation of resources for estimating cytomorphometric parameters would be to increase the number of culture flasks.

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7

Nowak, Raphaël. "Dick Hebdige, Sous-culture : Le sens du style." Volume !, no. 7 : 1 (May 15, 2010): 291–93. http://dx.doi.org/10.4000/volume.1124.

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8

Bennett, Andy. "Pour une réévaluation du concept de contre-culture." Volume !, no. 9 : 1 (September 15, 2012): 19–31. http://dx.doi.org/10.4000/volume.2941.

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9

Escande-Gauquié, Pauline, and Noémie Vermoesen. "« Critique de la culture et musiques populaires enregistrées »." Volume !, no. 10 : 2 (June 10, 2014): 218–20. http://dx.doi.org/10.4000/volume.4140.

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10

D’Aquino, Brian, Julian Henriques, and Leonardo Vidigal. "A Popular Culture Research Methodology: Sound System Outernational." Volume !, no. 13 : 2 (April 21, 2017): 163–75. http://dx.doi.org/10.4000/volume.5249.

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11

Perticoz, Lucien. "Jeremy Wade Morris, Selling Digital Music, Formatting Culture." Volume !, no. 15 : 1 (December 5, 2018): 167–69. http://dx.doi.org/10.4000/volume.5823.

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12

Harewood, Freya C., Nigel Curtis, Andrew J. Daley, Penelope A. Bryant, Amanda Gwee, and Thomas G. Connell. "Adequate or Inadequate? The Volume of Blood Submitted for Blood Culture at a Tertiary Children’s Hospital." Clinical Pediatrics 57, no. 11 (2018): 1310–17. http://dx.doi.org/10.1177/0009922818778042.

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The volume of blood sampled for culture critically influences the results. This study aimed to determine (1) the volume of blood submitted for culture, (2) the proportion of blood cultures with adequate volume, (3) whether measured improvement from a previous educational intervention had been sustained, and (4) the impact of blood volume on culture result. The volume of blood submitted for cultures was determined over a 13-month period by weighing bottles before and after collection and before and after an educational intervention. The volume of blood submitted in 5127 culture bottles were measured. Fewer than 50% of all cultures were deemed adequate. A significant pathogen was isolated in 4.7% of blood cultures, and low-volume cultures were more likely to yield contaminant isolates (47/2422 [1.9%] vs 22/2705 [0.8%], P = .0005). Subsequently, the higher rate of contaminant isolates from low-volume cultures may affect selection and rationalization of antibiotic therapy.

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13

Kim, Kyoungbo, and Sunggyun Park. "Validation of the Accuracy of Automatic Measurement of Blood Volume in Culture Bottles for Blood Culture." Diagnostics 13, no. 16 (2023): 2685. http://dx.doi.org/10.3390/diagnostics13162685.

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Several manufacturers have developed systems that automatically measure the amount of blood in culture bottles. We compared the volumes measured automatically by the Virtuo instrument (bioMérieux, France) (height-based volumes) and those calculated by weighing the bottles. In all, 150 pairs of aerobic and anaerobic blood culture bottles (BacT/ALERT FA/FN Plus, bioMérieux) were randomly selected over two periods to compare the height- and weight-based volumes and analyze the effect of foam. We also estimated the limit of detection (LOD) and the cut-off value for 5 mL equine blood. The mean height-based volume was approximately 0.67 mL greater than the weight-based volume, particularly in anaerobic culture bottles. Foam did not have a significant effect. The LOD for the automatic height-based volume of equine blood was 0.2–0.4 mL. The 5 mL cut-off was 4–4.2 mL. Therefore, when reporting or monitoring blood volume within culture bottles in the laboratory, these performance characteristics should be adequately considered.

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14

Rouzé, Vincent. "Michael Bull, Sound Moves. Ipod Culture and Urban Experience." Volume !, no. 10 : 1 (December 30, 2013): 308–11. http://dx.doi.org/10.4000/volume.3591.

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15

Mueller, Alain. "Fabien Hein, Do It Yourself : Autodétermination et culture punk." Volume !, no. 10 : 2 (June 10, 2014): 233–35. http://dx.doi.org/10.4000/volume.4099.

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Howard, Dennis. "Carolyn Cooper, Sound Clash: Jamaican Dancehall Culture at Large." Volume !, no. 13 : 2 (April 21, 2017): 222–25. http://dx.doi.org/10.4000/volume.5197.

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Cooper, Carolyn. "Incarner l’émancipation : marronnages érotiques dans la culture dancehall jamaïcaine." Volume !, no. 13 : 2 (April 21, 2017): 117–27. http://dx.doi.org/10.4000/volume.5216.

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Grassy, Elsa. "Deena Weinstein, Heavy Metal: The Music and Its Culture." Volume !, no. 5 : 2 (September 15, 2006): 187–90. http://dx.doi.org/10.4000/volume.563.

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Barber, Nicolette, Ioannis Valoumas, Krystal R. Leger, et al. "Culture, prefrontal volume, and memory." PLOS ONE 19, no. 3 (2024): e0298235. http://dx.doi.org/10.1371/journal.pone.0298235.

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Prior cross-cultural studies have demonstrated differences among Eastern and Western cultures in memory and cognition along with variation in neuroanatomy and functional engagement. We further probed cultural neuroanatomical variability in terms of its relationship with memory performance. Specifically, we investigated how memory performance related to gray matter volume in several prefrontal lobe structures, including across cultures. For 58 American and 57 Taiwanese young adults, memory performance was measured with the California Verbal Learning Test (CVLT) using performance on learning trial 1, on which Americans had higher scores than the Taiwanese, and the long delayed free recall task, on which groups performed similarly. MRI data were reconstructed using FreeSurfer. Across both cultures, we observed that larger volumes of the bilateral rostral anterior cingulate were associated with lower scores on both CVLT tasks. In terms of effects of culture, the relationship between learning trial 1 scores and gray matter volumes in the right superior frontal gyrus had a trend for a positive relationship in Taiwanese but not in Americans. In addition to the a priori analysis of select frontal volumes, an exploratory whole-brain analysis compared volumes—without considering CVLT performance—across the two cultural groups in order to assess convergence with prior research. Several cultural differences were found, such that Americans had larger volumes in the bilateral superior frontal and lateral occipital cortex, whereas Taiwanese had larger volumes in the bilateral rostral middle frontal and inferior temporal cortex, and the right precuneus.

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Blasquiz, Klaus, and Philippe Gonin. "La contre-culture en France : un entretien avec Klaus Blasquiz." Volume !, no. 9 : 2 (December 15, 2012): 125–32. http://dx.doi.org/10.4000/volume.3242.

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21

Tandt, Christophe Den. "La Culture rock entre utopie moderniste et construction d’une industrie alternative." Volume !, no. 9 : 2 (December 15, 2012): 15–30. http://dx.doi.org/10.4000/volume.3247.

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22

Hein, Fabien. "Keith Kahn-Harris, Extreme Metal. Music and Culture on the Edge." Volume !, no. 5 : 2 (September 15, 2006): 183–87. http://dx.doi.org/10.4000/volume.560.

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Moore, Ryan. "« Break on Through » : Contre-culture, musique et modernité dans les années 1960." Volume !, no. 9 : 1 (September 15, 2012): 33–49. http://dx.doi.org/10.4000/volume.3022.

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24

Pickard, Sarah. "Les Beatles et la naissance de la culture jeune en Grande-Bretagne." Volume !, no. 12 : 2 (March 22, 2016): 55–73. http://dx.doi.org/10.4000/volume.4799.

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Nowak, Raphaël. "Fréderic Martel, Mainstream. Enquête sur cette culture qui plaît à tout le monde." Volume !, no. 9 : 1 (September 15, 2012): 213–15. http://dx.doi.org/10.4000/volume.3139.

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26

Hawkins, Peter. "Diana Holmes & David Looseley (eds.), Imagining the popular in contemporary French culture." Volume !, no. 12 : 1 (November 30, 2015): 177–78. http://dx.doi.org/10.4000/volume.4677.

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27

Lamontagne, Samuel. "Graham St John (ed.), Weekend Societies, Electronic Dance Music Festivals and Event-Culture." Volume !, no. 14 : 2 (April 26, 2018): 278–79. http://dx.doi.org/10.4000/volume.5653.

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28

Fornäs, Johan, Martin Fredriksson, and Naomi Stead. "Culture Unbound Volume 6, Editorial." Culture Unbound 6, no. 1 (2014): 7–11. http://dx.doi.org/10.3384/cu.2000.1525.1467.

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With this volume, Culture Unbound celebrates its five-year anniversary. This makes a good opportunity both to look back at what we have achieved and to gaze ahead to what we have planned for the future. This new volume, which will be more extensive and ambitious than ever, thus marks a readiness and willingness to engage with some of the most acute problems and complex transformation that society faces. We hope and believe that this not only expresses the ambitions of Culture Unbound but also reflects a more general tendency within contemporary cultural research. In order to better accommodate the most recent developments within the field of cultural research, and facilitate intellectual discussion and critical analysis of contemporary issues we also plan to expand our repertoire of published material. In the coming year Culture Unbound will therefore introduce a section of texts we have chosen to call ‘Unbound Ideas’. Here we welcome academic essays and texts of a somewhat shorter format and freer approach to scholarly convention than our usual full-length research articles. These essays will take different – perhaps speculative or conjectural – positions, or give a new perspective on pressing topics or recently emerged concerns within cultural research.

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Déon, Maxence. "Paul E. Winters, Vinyl Records and Analog Culture in the Digital Age: Pressing Matters." Volume !, no. 18 : 1 (April 1, 2021): 152–55. http://dx.doi.org/10.4000/volume.9176.

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Rolland, Michaël. "Claude Chastagner, Révoltes et utopies. Militantisme et contre-culture dans l’Amérique des années soixante." Volume !, no. 9 : 1 (September 15, 2012): 173–74. http://dx.doi.org/10.4000/volume.3111.

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Chu, Meng Tze. "Qu’est-ce qu’une culture locale ? La pratique et l’expression du black metal à Taïwan." Volume !, no. 5 : 2 (September 15, 2006): 53–74. http://dx.doi.org/10.4000/volume.485.

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32

Sewell, David L., Thomas A. Golper, Peter B. Hulman, et al. "Comparison of Large Volume Culture to Other Methods for Isolation of Microorganisms from Dialysate." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 10, no. 1 (1990): 49–52. http://dx.doi.org/10.1177/089686089001000113.

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Patients on continuous ambulatory peritoneal dialysis (CAPD) who reside long distances from a CAPD center often use community medical laboratories to document and manage episodes of peritonitis. We examined the feasibility of using large volume cultures as an alternative to more costly and labor intensive methods and to enhance earlier recovery of microorganisms from these patients. Three methods of processing dialysate from patients on CAPD were compared: (a) inoculation of 400 mL dialysate into a transfer bag (Baxter Healthcare, Inc., Round Lake, IL) containing 100 mL of 5-fold concentrate of trypticase-soy broth: (b) inoculation of 5 mL into each of two Bactec bottles (Johnston Laboratories, Towson, MD): and (c) centrifugation of 50 mL and culture of the sediment without white cell lysis on plated media and two Bactec bottles. Of the 58 specimens cultured, 34 (59%) were positive by one or more methods. Antimicrobial activity was detected in 20158 (34%) dialysates, which represent 54% of all no-growth cultures. Of the 34 culture-positive specimens, microorganisms were recovered on plated media in 22 (65%); by the centrifugation system in 32 (94%); by the routine Bactec system in 28 (82%); and by large volume culture in 30 (88%). The large volume culture system is an acceptable alternative to the more costly Bactec System and the labor intensive centrifugation method but does not significantly improve recovery of microorganisms.

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33

Guibert, Gérome. "Benoît Domergue, Culture barock et gothic flamboyant. La musique extrême : un écho surgi des abîmes." Volume !, no. 1 : 1 (May 15, 2002): 126–27. http://dx.doi.org/10.4000/volume.2546.

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34

Sidhu, Karatatiwant Singh, Eyal Amiel, Ralph C. Budd, and Dwight E. Matthews. "Determination of cell volume as part of metabolomics experiments." American Journal of Physiology-Cell Physiology 321, no. 6 (2021): C947—C953. http://dx.doi.org/10.1152/ajpcell.00613.2020.

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Cells regulate their cell volume, but cell volumes may change in response to metabolic and other perturbations. Many metabolomics experiments use cultured cells to measure changes in metabolites in response to physiological and other experimental perturbations, but the metabolomics workflow by mass spectrometry only determines total metabolite amounts in cell culture extracts. To convert metabolite amount to metabolite concentration requires knowledge of the number and volume of the cells. Measuring only metabolite amount can lead to incorrect or skewed results in cell culture experiments because cell size may change due to experimental conditions independent of change in metabolite concentration. We have developed a novel method to determine cell volume in cell culture experiments using a pair of stable isotopically labeled phenylalanine internal standards incorporated within the normal liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics workflow. This method relies on the flooding-dose technique where the intracellular concentration of a particular compound (in this case phenylalanine) is forced to equal its extracellular concentration. We illustrate the LC-MS/MS technique for two different mammalian cell lines. Although the method is applicable in general for determining cell volume, the major advantage of the method is its seamless incorporation within the normal metabolomics workflow.

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35

Tisserat, Brent. "Growth Responses and Construction Costs of Various Tissue Culture Systems." HortTechnology 6, no. 1 (1996): 62–68. http://dx.doi.org/10.21273/horttech.6.1.62.

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The influence of the culture chamber size and medium volume on the growth rates of shoot tips of peas, lettuce, kidney beans, and spearmint were determined after 8 weeks of incubation. Cultures were grown in a variety of culture chambers including culture tubes, baby food jars, Magenta GA-7 containers, 1-pint Mason jars, 1-quart Mason jars used with and without an automated plant culture system (APCS), 0.5-gal Mason jars with and without an APCS, Bio-safe chambers with an APCS, and polycarbonate culture chambers with an APCS having culture chamber volumes of 55, 143, 365, 462, 925, 1850, 6000, and 16,400 ml, respectively. Plans are presented for the construction of various culture chambers used in an APCS. The APCS consisted of a peristaltic pump, media reservoir containing 1 liter of liquid nutrient medium, and a culture chamber. Cultures grown with an APCS consistently produced higher fresh weights than cultures using any of the agar culture systems tested. Growth rates varied considerably depending on the plant species and culture system tested. Peas, lettuce, and spearmint exhibited flowering only when grown in the APCS. A cost comparison using the APCS versus various conventional tissue culture systems is presented.

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Hashiyada, Y., H. Takahashi, D. Yamaguchi, K. Imai та M. Geshi. "74 EFFECT OF SIZE AND CULTURE PERIOD OF FROZEN–THAWED BOVINE TROPHOBLASTIC VESICLES ON INTERFERON-τ SECRETION". Reproduction, Fertility and Development 26, № 1 (2014): 151. http://dx.doi.org/10.1071/rdv26n1ab74.

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Frozen–thawed bovine trophoblastic vesicles (bTV) derived in vivo could secrete interferon-τ (IFN-τ) at the same level as fresh bTV on Days 4 to 6 after thawing. However, amounts of IFN-τ decreased following continuous in vitro culture (Hashiyada et al. 2012 38th IETS). Co-transfer of frozen–thawed bTV improved pregnancy rate of embryos due to the effects of IFN-τ secreted by bTV (Hashiyada et al. 2008 41th SSR). However, the relation between bTV size and IFN-τ secretion level during culture has not been well documented. The objective of present study was to characterise the concentration of IFN-τ related bTV volume and culture period after thawing of cryopreserved bTV. The bTV were prepared from Day 16 elongating blastocysts recovered nonsurgically. The dissected trophoblastic fragment, 1 to 1.5 mm in width, was cultured using TCM-199 supplemented with 20% (vol/vol) fetal bovine serum and 0.1 mM β-mercaptoethanol. Formed vesicles after 24 h of culture were cryopreserved using D-PBS supplemented with 20% calf serum and 1.8 M ethylene glycol. After thawing, bTV were cultured individually with 100 μL/well/day until Day 2 (i.e. the day of thawing was defined as Day 0), and thereafter changed to 200 μL/well/day to termination at Day 10. Collection of culture media and measurement of bTV diameter were performed before cryopreservation and after thawing for every day. Interferon-τ in collected media was measured by radioimmunoassay. The estimated bTV volume was calculated based on the diameter. Data were analysed by Student's t-test. Nine fresh bTV before cryopreservation were used to assess the IFN-τ secretion for 24 h in relation to bTV volume. A significant positive correlation was observed between secreted IFN-τ (mean ± s.e.M, 19.9 ± 3.1 ng mL–1) and bTV volume (1.49 ± 0.6 mm3, r = 0.91; P < 0.01). Initial IFN-τ secretion from bTV cultured for 24 h after thawing was significantly decreased compared with that before cryopreservation (29.1 ± 2.1 ng mL–1 and 58.4 ± 4.8 ng mL–1; P < 0.01, n = 27). In continuous culture of bTV (n = 8), IFN-τ secretion increased gradually from Day 2 (23.1 ± 9.0 ng mL–1) to Day 4 (32.2 ± 8.4 ng mL–1), and then maintained this level until Day 7 (33.4 ± 14.9 ng mL–1). However, this amount of IFN-τ tended to decrease on Day 8 (24.8 ± 5.0 ng mL–1), 9 (16.5 ± 4.4 ng mL–1), and 10 (12.0 ± 1.7 ng mL–1). Interferon-τ secretion from bTV on Day 9 and 10 was lower than that on Day 3, 4, 5, 6, and 8, respectively (P < 0.05). Volume of bTV increased also from Day 2 (0.2 ± 0.1 mm3) to Day 5, 6 (0.8 ± 0.3 mm3) and 7 (0.7 ± 0.2 mm3). However, bTV volumes shrank drastically on Day 8 (0.3 ± 0.1 mm3), 9, and 10 (0.2 ± 0.1 mm3). In comparison with bTV during culture, volumes on Day 4, 5, and 7 were greater than those on Day 2 and 3, and volumes on Day 6 and 7 were greater than on Day 8, 9, and 10 (P < 0.05). These results indicate that the dynamics of IFN-τ secretion reflected the expansion or reduction of bTV in continued culture after thawing. Interferon-τ secretion might be related to bTV volume. Moreover, we reconfirmed that cryopreserved bTV highly express IFN-τ during 4 to 7 days after thawing.

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37

Perchard, Tom. "Eric Drott, Music and the Elusive Revolution: Cultural Politics and Political Culture in France, 1968-1981." Volume !, no. 9 : 2 (December 15, 2012): 150–52. http://dx.doi.org/10.4000/volume.3398.

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Herbert, Ruth. "Harris M. Berger, Stance: Ideas About Emotion, Style, and Meaning for the Study of Expressive Culture." Volume !, no. 10 : 1 (December 30, 2013): 301–3. http://dx.doi.org/10.4000/volume.3583.

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Mitchell, Tony. "Alain-Philippe Durand (ed.), Black, Blanc, Beur : Rap Music and Hip-Hop Culture in the Francophone World." Volume !, no. 3 : 2 (October 15, 2004): 144–49. http://dx.doi.org/10.4000/volume.1992.

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Harbord, Jack. "Christopher J. Smith, The Creolization of American Culture: William Sidney Mount and the Roots of Blackface Minstrelsy." Volume !, no. 11 : 2 (June 15, 2015): 170–72. http://dx.doi.org/10.4000/volume.4517.

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41

Baker, J. R., and K. Maramorosch. "Advances in Cell Culture, Volume 4." Journal of Parasitology 72, no. 4 (1986): 549. http://dx.doi.org/10.2307/3281506.

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Kusama, Yoshiki, Nobuaki Shime, Kenta Ito, Yusuke Ito, and Masashi Kasai. "The Volume of Pediatric Blood Culture." Pediatric Infectious Disease Journal 38, no. 12 (2019): e340-e341. http://dx.doi.org/10.1097/inf.0000000000002466.

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Piatti-Farnell, Lorna, Ashleigh Prosser, and Gwyneth Peaty. "Critical intersections in popular culture." Australasian Journal of Popular Culture 12, no. 2 (2023): 107–11. http://dx.doi.org/10.1386/ajpc_00072_2.

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In this editorial, the editors introduce the 12.2 volume of The Australasian Journal of Popular Culture. The dynamic and interdisciplinary nature of the field is discussed with reference to the collection of articles within the volume, highlighting the malleability of popular culture in all its transdisciplinary forms. The editors provide a summary of the seven articles included in the volume, which collectively represent diverse critical discussions of the field across sociopolitical, socioeconomic and sociocultural artistic realms. The articles examine the evolving realms of the monstrous, the mythic, the heroic and the historical through various mediums like television, film, characters and historical moments. The editors then conclude by offering a summary of the three book reviews included in the volume.

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Qian, Fang, Shu Juan Jiang, Tao Liu, and Guang Qing Mu. "Optimization of Culture Medium and Conditions for Bacillus coagulans T242 Producing Thermostable Lactase." Advanced Materials Research 634-638 (January 2013): 1259–62. http://dx.doi.org/10.4028/www.scientific.net/amr.634-638.1259.

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A thermotolerant lactase-producing strain Bacillus coagulans T242 was obtained in our previous work, its media and culture conditions were investigated and optimized for enhanced production of lactase in the present work. Results showed medium containing 1.5% lactose, 1.0% peptone, 1.5% yeast extract, 0.7% MgSO4, and pH 8.0, was the optimum medium; And its optimum culture conditions were the age of bacterial culture 36 h, inoculation volume 2%, liquid medium volume loaded 30 mL/250 mL flask, 60 °C, 36 h. When cultured at the optimum condition Bacillus coagulans T242 yielded the maximum of 1.21U/mL lactase activity.

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Sipaúba-Tavares, LH, RN Millan, and FA Berchielli-Morais. "Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow." Brazilian Journal of Biology 73, no. 3 (2013): 585–91. http://dx.doi.org/10.1590/s1519-69842013000300016.

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Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 105 cells.mL−1 on the 12th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions.

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Procop, Gary W., Suzanne K. Nelson, Barbara J. Blond, Rhona J. Souers, and Larry W. Massie. "The Impact of Transit Times on the Detection of Bacterial Pathogens in Blood Cultures: A College of American Pathologists Q-Probes Study of 36 Institutions." Archives of Pathology & Laboratory Medicine 144, no. 5 (2019): 564–71. http://dx.doi.org/10.5858/arpa.2019-0258-cp.

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Context.— Consolidation of clinical microbiology laboratory services has resulted in extended transit time for blood cultures from service points distant from the laboratory. Sepsis is critical; delays in identification of etiologic agents of diseases could adversely impact patient care. Objective.— To examine the effect of total preanalytic time and blood culture volume on the instrument time-to-detection for bacterial pathogens in blood cultures. A secondary objective was to obtain relevant blood culture information by questionnaire. Design.— Participants in this Q-Probes study recorded date, time, and volume information for the first 50 positive blood cultures collected during the 12-week study period. Additional information regarding blood culture collection practices was obtained through questionnaire. Results.— Prolonged overall time-to-detection was secondary to prolonged preanalytic time, particularly prolonged transit time, rather than slower organism growth once bottles were placed on the instrument. Among 1578 blood cultures, the overall time from collection to positive result was significantly less for blood cultures collected on-site than for off-site locations. Most institutions lack sufficient training programs and do not monitor preanalytic time metrics associated with blood cultures. Four hundred fifty-six of the 1580 blood cultures with blood volume adequacy reported (28.9%) were inadequately filled. Conclusions.— Overall process time (specimen collection to positive blood culture detection) is predicted to be higher for blood cultures collected off-site. Transit time is a variable that can be reduced to decrease overall time to detection. Thus, improved training and closer attention to preanalytic metrics associated with blood cultures could decrease hospital stays and mortality rates.

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Gross, Jonathan, Sarit Avrani, Sophia Katz, Sabrin Hilau, and Ruth Hershberg. "Culture Volume Influences the Dynamics of Adaptation under Long-Term Stationary Phase." Genome Biology and Evolution 12, no. 12 (2020): 2292–301. http://dx.doi.org/10.1093/gbe/evaa210.

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Abstract Escherichia coli and many other bacterial species, which are incapable of sporulation, can nevertheless survive within resource exhausted media by entering a state termed long-term stationary phase (LTSP). We have previously shown that E. coli populations adapt genetically under LTSP in an extremely convergent manner. Here, we examine how the dynamics of LTSP genetic adaptation are influenced by varying a single parameter of the experiment—culture volume. We find that culture volume affects survival under LTSP, with viable counts decreasing as volumes increase. Across all volumes, mutations accumulate with time, and the majority of mutations accumulated demonstrate signals of being adaptive. However, positive selection appears to affect mutation accumulation more strongly at higher, compared with lower volumes. Finally, we find that several similar genes are likely involved in adaptation across volumes. However, the specific mutations within these genes that contribute to adaptation can vary in a consistent manner. Combined, our results demonstrate how varying a single parameter of an evolutionary experiment can substantially influence the dynamics of observed adaptation.

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Jinadatha, Chetan, Eileen M. Stock, Steve E. Miller, and William F. McCoy. "Environmental Validation of Legionella Control in a VHA Facility Water System." Infection Control & Hospital Epidemiology 39, no. 3 (2018): 259–66. http://dx.doi.org/10.1017/ice.2017.318.

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OBJECTIVESWe conducted this study to determine what sample volume, concentration, and limit of detection (LOD) are adequate for environmental validation of Legionella control. We also sought to determine whether time required to obtain culture results can be reduced compared to spread-plate culture method. We also assessed whether polymerase chain reaction (PCR) and in-field total heterotrophic aerobic bacteria (THAB) counts are reliable indicators of Legionella in water samples from buildings.DESIGNComparative Legionella screening and diagnostics study for environmental validation of a healthcare building water system.SETTINGVeterans Health Administration (VHA) facility water system in central Texas.METHODSWe analyzed 50 water samples (26 hot, 24 cold) from 40 sinks and 10 showers using spread-plate cultures (International Standards Organization [ISO] 11731) on samples shipped overnight to the analytical lab. In-field, on-site cultures were obtained using the PVT (Phigenics Validation Test) culture dipslide-format sampler. A PCR assay for genus-level Legionella was performed on every sample.RESULTSNo practical differences regardless of sample volume filtered were observed. Larger sample volumes yielded more detections of Legionella. No statistically significant differences at the 1 colony-forming unit (CFU)/mL or 10 CFU/mL LOD were observed. Approximately 75% less time was required when cultures were started in the field. The PCR results provided an early warning, which was confirmed by spread-plate cultures. The THAB results did not correlate with Legionella status.CONCLUSIONSFor environmental validation at this facility, we confirmed that (1) 100 mL sample volumes were adequate, (2) 10× concentrations were adequate, (3) 10 CFU/mL LOD was adequate, (4) in-field cultures reliably reduced time to get results by 75%, (5) PCR provided a reliable early warning, and (6) THAB was not predictive of Legionella results.Infect Control Hosp Epidemiol 2018;39:259–266

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Bouce, Paul-Gabriel, John Yolton, and Leslie Ellen Brown. "Studies in Eighteenth-Century Culture. Volume XVII." Modern Language Review 86, no. 2 (1991): 392. http://dx.doi.org/10.2307/3730542.

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Mante, Seth, W. R. Sharp, D. A. Evans, P. V. Ammirato, and Y. Yamada. "Handbook of Plant Cell Culture. Volume 2." Brittonia 37, no. 2 (1985): 198. http://dx.doi.org/10.2307/2806109.

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