Academic literature on the topic 'Water quality – KwaZulu-Natal – Umgeni River'

Located in the province of KwaZulu-Natal, South Africa, the Mgeni River catchment provides the potable water resources for nearly two million people, in an area of approximately 4 400 km2. The river system is well developed and impounded, but increasing population pressures in the catchment are placing greater demands on this critical water resource resulting in increasing loads of nutrients, faecal bacteria and suspended solids. Umgeni Water and the Department of Water Affairs and Forestry are developing an affordable and practically implementable plan to manage the water quality on an integrated catchment basis, encompassing demographic, land-use and management considerations. The development of the plan is discussed and information on the water management related issues provided. A multidisciplinary approach has proved essential, facilitated by the development of a GIS-based system to manage and utilize the information from the study. Establishment of communication links with interested and affected parties has been critical, as has the clarification of their roles and responsibilities in dealing with identified pollution issues. Future development within the catchment is considered and the implementation of the management plan is discussed against the background of the Reconstruction and Development Programme.

A project was designed by Umgeni Water (funded by the Water Research Commission of South Africa) to monitor the implementation of water reticulation in Vulindlela and evaluate the Water Supply Scheme from a community and environmental health perspective. The findings would hopefully contribute toward the development of criteria for Health Impact Assessment on Water Reticulation Projects. One objective of this study was to evaluate the usefulness of diarrhoeal disease as opposed to other health indicators for water-associated diseases. The innovative methodology followed in the study, a “stepped wedge design”, compared four discrete areas of water reticulation implementation in Vulindlela over a 15-month period. Five surveys, including a baseline and four follow-ups at each household, were carried out. Analysis (microbiological, chemical) was carried out of samples from the household drinking water and from the source of the water. Each survey included health questionnaires, the respondent being the head of the household in each case. Overall, there was no direct correlation proved between water quality and diarrhoea per se. However, there was a marked decrease in diarrhoea with the introduction of the new water supply. There was definite correlation between hygiene behaviours and diarrhoea. Diarrhoea would seem to be the health impact associated with water, of choice.

Three data sources (physico-chemistry, bio-monitoring and eco-toxicology) are currently used in South Africa to establish environmental water quality conditions. Environmental water quality in turn is key information required for the “ecological reserve determination” of river reaches. Bio-monitoring in South Africa has been limited in recent times mostly to the use of the SASS procedure which relies on invertebrates only. This paper describes the re-introduction of a diatom-based water quality assessment as an added-value bio-monitoring tool. A specific example is discussed citing the response of diatom assemblages to diffuse pollution from acid mine drainage and how effective diatoms are as indicators of ecological integrity and river recovery measured downstream of the area of impact. The advantages of applying this bio-monitoring technique over other biological measures are presented in the context of technological advances in rapid image processing, species identification and software applications of diatom-based water quality indices. The valuable records of the diatom assemblages of the past, held in the South African Diatom Collection at the CSIR (KwaZulu-Natal), can now be accessed and interpreted as historical environmental water quality reference points for several rivers in South Africa.

Abstract. Water shortages are a chronic and severe problem in South Africa. Allocation of this limited water resources, environmental quality, and policies for sustainable water use are issues of increasing concern that require accurate and timely information to evolve strategies for dynamic natural resources management. Specifically, this paper is aimed to assist the planning, restoring and to rationally allocate the water resources in any river basin in resolving the current water stresses in many parts of South Africa, by using integrated knowledge from simulation and integrated river basin management approach. The developed system dynamic (SD) allocation system was used to investigates the extent to which the framework is ‘sustainable’ in the medium and long terms in evaluating existing and future water allocation among conflicting users at Mkomazi River Basin (MRB), KwaZulu-Natal Province, South Africa The invented SD framework confirms agricultural water use as the highest demand when compared with other users. The optimal sustainability performance index (0.25) of the system at 70% dependable flow shows an integrated scenario that combines rainfall variation with improved irrigation water use efficiency as a suitable framework plan. The study uses integrated knowledge from simulation and integrated river basin management approach as a feasible method to assist the planning, restoring and to rationally allocate the water resources in any river basin with similar attributes to the study area in resolving the current water stresses in many parts of the country. Water resources managers would find these tools beneficial in understanding the complex nature of water resources allocation and in determining priorities area which required prompt attention and intervention.

This study assessed the quality of the Umgeni River in Durban South Africa seasonally from March 2011 to January 2012, according to standard protocol. Water samples were collected from Inanda dam-U5, KrantzKloof Nature Reserve-U4, New Germany-U3, Reservoir Hills –U2 and River mouth – U1 areas of the Umgeni River. A two-step tangential flow filtration (TFF) process was setup for the concentration of viruses from water samples. Virus like particles (VLPs) was detected using electron microscopy. Canonical correspondence analysis (CCA) was used to statistically evaluate the data sets. All water samples had turbidity values which exceeded the South African water quality guideline value of 0.1 NTU for turbidity. Large seasonal variations in BOD5, COD and conductivity levels were observed. Chloride concentrations were extremely high at point U1 (19234 mg/ℓ) Cl during summer. Total heterotrophic bacterial (THB) population was highest at 13.67 x 106 cfu/100ml (U1 – summer). Enterococci (EC) concentrations were detected at points U1, U2, U3, and U4 during the autumn and spring period. pH, electrical conductivity, temperature, and turbidity positively correlated with the microbial communities, and were the key parameters responsible for water pollution according to CCA. Most water samples contained high populations of somatic (659 pfu/mℓ, U1 – summer) and F-RNA coliphages (550 pfu/mℓ, U2 – summer). VLPs were detected throughout all seasons, with point U1 (summer) having the highest population of 2086 VLP/mℓ. Several presumptive viruses including Adenoviridae, Picornaviridae, Poxviridae, and Reoviridae were detected based on their morphologies. Six cell culture lines were used to determine cytopathic effect (CPE) of the VLPs. VLP samples produced CPEs on the Vero, Hek 293, Hela and A549 cell lines. Integrated cell culture (ICC) - PCR confirmed the presence of infectious VLPs in the river water samples. Adenoviruses, Enteroviruses, rotaviruses and Hepatitis B viruses were detected and quantified in all water samples by nested PCR/RT-PCR and Real-Time PCR respectively, against positive control viruses. These results indicate the potential of viruses in the water samples especially from the lower catchment areas to infect the human hosts throughout the year. These observations have public health care implications and establish a need to monitor the viral population in addition to traditional water quality indicators.
Thesis (Ph.D.)-University of KwaZulu-Natal, Westville, 2013.

Dissertation submitted in partial compliance with the requirements for the Master's Degree in Technology: Environmental Health, Technikon Natal, 2001.
The debate surrounding mercury pollution of the river system below Thor Chemicals in Kwa Zulu Natal, South Africa has been of concern to the people of the region for several years. No scientific assessment of Mercury pollution has been carried out to date, other then a study conducted by Johnston et al. (1991) in 1990 in an area restricted to within 5 kilometres of the plant. Due to elevated levels being detected directly below the plant, Johnston et al. (1991) have expressed concern that this could result in the mobilisation of Mercury into the ecosystem and possibly threaten the Ecology of the Inanda Dam which is located approximately 20 kilometres south of Thor Chemicals which is a major reservoir for Durban. Added to this, the community residing in the area has expressed a great deal of concern and the matter was taken up in parliament in 1998. These factors provide impetus for a follow up study to determine the extent of mercury transformation into the higher trophic levels in the area surrounding Thor Chemicals. In order to quantify the extent to which mercury has become mobilised in the ecosystem and to identify possible pathways of exposure to the community, samples of sediment, algae, cattle hair and fish were taken at ten sample sites. These sample sites were selected at intervals along the Mngceweni and Umgeni River. The first sampling site was situated below Thor and the final sample site was located at the head of the Inanda Dam. Sediment, algae, cattle hair and fish were specifically chosen as they are considered to be reliable indicators of mercury pollution and mobilization. Fish,form an important part of the diet of the community residing in the study area. Mercury concentrations for the above parameters, were compared to mercury concentrations found in the control area, upstream from Thor, as well as to international and local standards. Composite sediment samples taken at the first sample site, within 500 metres of Thor Chemicals, revealed a mercury level of 54j.lg/gram. Mercury concentrations detected in the remainder of the composite sediment samples were significantly lower and revealed similar levels of magnitude throughout the rest of the study area. The higher concentration of mercury in the sediment at the site directly below Thor Chemicals may be attributed to a high
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The health and life expectancy of populations in developing countries is largely determined by the availability of good quality drinking water. Boreholes and springs generally provide water of better microbiological and physical quality than surface water sources, however, they may cause health and aesthetic problems due to chemical constituents dissolved out of the host rock. As part of a pilot study to assess the health-related quality of community water supplies, samples were taken from two Quaternary catchment areas in KwaZulu-Natal. The Umkomazi catchment area is located inland from Amanzimtoti, while the Umfolozi catchment area is located north-east of Ulundi. The geology in these areas is significantly different. The Umkomazi area is predominantly underlain by basement rocks of the Natal Structural and Metamorphic Province, while the Umfolozi area is underlain by sedimentary rocks of the Karoo Supergroup. Geographical information systems (GIS) were used to examine the influence of lithology, rainfall and landuse activities on groundwater quality. Major ion analysis of groundwater samples from the Umkomazi area revealed a linear relationship between borehole and spring concentrations. Dwyka Tillite was found to produce water with the highest concentrations of major ions, while Karoo dolerite produced water with the lowest concentrations of major ions. Samples from basement rocks and Natal Group contained intermediate concentrations of major ions. In the Umfolozi area Karoo dolerite samples showed the lowest concentrations of major ions, while the Vryheid Formation and Dwyka Tillite produced the highest borehole and spring concentrations, respectively. High salinity levels in sedimentary rocks may be due to marine influence during deposition. Piper diagrams show relative enrichment of major cations and anions and Stiff diagrams showed characteristic patterns. Fluoride is associated with siliceous basement rocks and related to calcium concentrations through the solubility of calcium fluoride. The trace metals, manganese, iron and zinc were found to cause significant aesthetic problems and possibly health problems in sensitive individuals. These constituents are derived from weathering of bedrock and possibly from the corrosion of metal pipes. There is an inverse relationship between mean annual rainfall (MAR) and electrical conductivity (EC), except near the coast where windblown salinity increases with rainfall. Nitrate, ammonium and E. Coli contamination are linked to landuse activities such as occurrence of human and animal excreta near the water source and the proximity of pit latrines. It is recommended that rural communities be educated about the nature and importance of groundwater quality.
Thesis (M.Sc.)-University of Natal, Durban, 1998.

This study represents an integrated and holistic assessment of the eziMbokodweni Estuary’s water and sediment quality. The estuary was once relatively un-impacted but now flows through a highly anthropogenically-modified catchment, comprising industrial and residential developments (formal and informal), and the eziMbokodweni Wastewater Treatment Works, furthermore the floodplain has been completely transformed to accommodate the Amanzimtoti golf course. Flow from the highly degraded Isipingo River and Estuary is occasionally diverted into the eziMbokodweni and the Southern Sewage Works Outfall, one of the largest deep sea sewage outfalls in the eThekwini Municipality, is located at sea, approximately 1.5 km south of the estuary mouth. Estuarine health can be studied on various fronts, in this study, the following variables were monitored in the water and sediment columns, seasonally for spring and neap tides: heavy metals, nutrients, bacteria and selected physico-chemical parameters. The results demonstrated that the eziMbokodweni catchment was a major contributor of heavy metals and nutrients to the estuarine system. The quantity of the majority of heavy metals and nutrients transported by the river at the upper estuary exceeded the amount exported to sea at the estuary mouth-nearshore interface, resulting in the estuary accruing large quantities of material seasonality. It was noted that the nearshore waters are enriched, as a variety of heavy metals and nutrients entered the estuary mouth during flood tide episodes. A range of heavy metals were detected within the sediment profiles obtained from the estuary, with fine to medium grained sediment exhibiting greater heavy metal content, in some cases, multi-fold higher than that detected in medium to coarse grained sediment. Geochemical indices were employed to ascertain the extent to which these metals constituted a pollution threat to the environment. The contamination factor calculated for all sediment layers and sampling sites implied low contamination. The enrichment factor calculated for the majority of heavy metals alluded to natural causes while some heavy metals exhibited significant to extremely high levels of enrichment thus implicating anthropogenic causes as likely sources of these heavy metals. The degree of contamination of the estuary was classified as low. Overall, the heavy metal and nutrient content detected in the sediments were low as compared to the large quantities detected from the budget. This indicates that natural mechanisms exist which facilitate the utilization or removal of these heavy metals and nutrients from the estuary. It is proposed that the most significant manner by which these heavy metals and nutrients are eliminated from the estuary is through episodic flood events that scour and strip fine grained sediment from the estuary bed. The pH assessment of the estuary revealed that this environment was slightly basic through most of the year with mildly acidic conditions noted during winter. The average Dissolved Oxygen levels were within acceptable levels, with the exception of winter when levels were very close to hypoxic conditions. The average Total Dissolved Solid content indicated that the estuary was compliant with the target water quality range for domestic use on selected seasonal-tidal cycles, and the average Electrical Conductivity levels were compliant with the ideal target water quality range for aquatic ecosystems and domestic use as per the South African water quality guidelines (DWAF, 1996a-e). A salinity gradient was evident in the estuary, as salinity levels decreased with distance from the estuary mouth. The saline nature of the lower estuary provided for the flocculation of material, which was alarming as large quantities of heavy metals and nutrients were detected in the estuary. The lower estuary is therefore susceptible to contamination due to the salinity regime and the dominance of fine grained sediment. The demand for oxygen in the estuary was high during summer, autumn and spring, and within acceptable levels for natural waters during winter. Extremely high levels of Chemical Oxygen Demand were recorded in the estuary which provides prime growth and survival opportunities for bacteria. This correlated with the results from microbiological investigations as high levels of bacteria were noted within the estuary, in both the sediment and water columns. The detected quantities of Total Coliforms, Faecal Coliforms and Escherichia Coliforms exceeded the target water quality thresholds for domestic and full contact recreational use. While at selected sites, the recorded Faecal Coliforms and Escherichia Coliforms levels were deemed compliant for intermediate contact recreational use. However, these sites were not compliant throughout the sampling period. Overall, the upper and mid-estuary exhibited greater counts of bacteria, in both the sediment and water column, than the estuary mouth and was attributed to factors such as flushing, sediment particle size, salinity and the ‘dilution effect’. Furthermore, the sediment of the eziMbokodweni Estuary is serving as a reservoir for bacteria. A number of options have been proposed to improve the degraded state of the eziMbokodweni Estuary and are ultimately aimed at curbing the anthropogenic input of these contaminants. The insight gained from this study provides the database from which more integrated and holistic estuarine management strategies can stem, that are applicable not just to the eziMbokodweni Estuary but estuaries in general.
M.Sc. University of KwaZulu-Natal, Durban 2013.

The Umdloti River is relatively small but very important system that is located northeast of central Durban. This river flows pass the coastal town of Verulam and finally into an open/closed estuary, the La Mercy estuary. This fluvial system has a concrete gravity dam that is built in the upper reaches, the Hazelmere Dam, which supplies water to the north local council and surrounding districts under the supervision of Umgeni Water. The river is characterized by human activities, especially urbanisation and industrialization in the middle reaches and intensive agriculture (vegetables, sugar cane and banana plantations) along the catchment. The influence of anthropogenic factors within this catchment results from the dam construction, informal settlements, both commercial and subsistence agriculture, intensive industrial activity, accelerated urban developments, and recreational uses. Consequently there has been considerable concern regarding the impacts of these factors together with natural influences on the water quality and health status of this fluvial system. In this study water samples were taken and analysed for the following variables: nitrites; nitrates; ammonia; pH; Escherichia coli; sulphate; phosphate; total dissolved solids; chemical oxygen demand; biological oxygen demand; calcium and magnesium. The results indicate that the middle and the lower reaches of the Umdloti River are most impacted. Further, results from a questionnaire survey indicate that natural and human induced impacts have impacted negatively on the health status of the Umdloti River. The questionnaire survey also revealed that respondents benefited positively from the construction of the dam whilst the expropriation of land for the construction of the dam itself created much resentment to the prior land owners. The water quality data and the questionnaire analysis indicate overall natural and human induced impacts have had negative effects on the Umdloti River and the La Mercy estuary. It is necessary that local municipal authorities introduce corrective catchment management practices (outlined in the final chapter of the study) to enhance the water quality and health status of the river.
Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2009.

The Reserve concept, introduced in the National Water Act, has led to an increasing use of biomonitoring techniques to assess the current ecological status of rivers in South Africa. The ecological status of a river provides vital information necessary to establish the amount and quality of water needed by the aquatic ecosystem. If the amount and quality of water is inadequate, then the water resource will not be able to provide the necessary goods and services to their respective water users. The biomonitoring of rivers gained impetus with the establishment of the River Health Programme (RHP) and its various objectives. The RHP led to the development and refinement of a range of biomonitoring indices to assess various components of the aquatic ecosystem. The components used to assess the current ecological status of a river include the physical habitat, riparian vegetation, water quality, as well as the macro-invertebrate and fish communities. The Mvoti River, in the vicinity of Stanger, is subjected to extensive water abstraction, which is then utilised for irrigation, industrial use, urban water requirements and various domestic uses by informal settlements. Previous studies on the river indicated that it is in a severely degraded state, especially below the confluences of the Nchaweni and Mbozambo rivers. Thus, this assessment of the current ecological status of the Mvoti River was undertaken to possibly identify the main causes of the degraded state. The study was undertaken during the high (February) and low flow (August) periods in 2005. Selected monitoring sites included sites used in previous studies on the Mvoti River as well as sites on the Nchaweni and Mbozambo tributaries. The methodology for this study was to implement the various indices used in the RHP for the different ecosystem components. The habitat indices implemented in this study were the Habitat Quality Index (HQI) and Integrated Habitat Assessment System (IHAS). Physicochemical water analyses were done during each sampling period to assess the water quality against the South African Water Quality Guidelines. The biotic indices used included the South African Scoring System 5 (SASS5) index to assess the macro-invertebrate community and the Fish Assemblage Integrity Index (FAII) to assess the fish community. Additionally, the newly developed EcoStatus indices for the fish and macro-invertebrate communities were implemented on the available fish and macro-invertebrate data. These indices were developed mainly to be used in Reserve determination assessments, where the assessments are built around the integration of all ecosystem components into a single value for the ecological status. The fish index is termed the Fish Response Assessment Index (FRAI) and the macro-invertebrate index the Macro-invertebrate Assessment Index (MIRAI)…. Multivariate statistical analyses were performed on the water quality as well as the macroinvertebrate and fish communities to establish any temporal and spatial trends together with any dominating water quality variables responsible for species composition at the different sites. PRIMER and CANOCO computer software were used to construct hierarchical clusters and NMDS plots for the biotic communities, while RDA and PCA bi-plots were used to represent the water quality variables and their effects on the biotic community structure. The Mvoti River is in a seriously degraded state and this degradation was particularly evident during the low flow period below the Sappi Stanger mill and the Stanger Sewage works effluent discharge points. The degraded state of the river is caused by the multitude of impacts on the river, which includes the local land-use, upstream land-use and effluent discharges in the vicinity of Stanger. The water quality in the vicinity of Stanger was in a poor state during the low flow, while the high flow was only slightly better. The upstream site is in a fair condition but, after the Nchaweni and Mbozambo rivers enter the Mvoti River, the water quality decreases. The variables found to be problematic included microbiological variables, chlorides and electrical conductivity in the Mvoti River. High nutrient values were found together with very high electrical conductivity in especially the Nchaweni River tributary. The lower Mvoti River contains very little habitat of good quality to support the biotic communities. The habitat is generally degraded due to the destruction of the riparian zone and the dominance of alien vegetation in the form of reeds. This, together with land-use and water abstraction activities, has caused high sediment loads in the lower Mvoti River which are continually moving. The IHAS and HQI results indicated the habitat in the lower Mvoti River is in a modified condition. The statistical analysis of the macro-invertebrate data showed that a definite spatial variation existed while no significant temporal variation was identified. There is a difference in the community structure between the Mvoti River and its tributaries, with the tributaries containing a very poor diversity. This lowered species diversity was attributed to the effects of, specifically, chlorides on the community structure but the origin of the chlorides could not be linked specifically to the Sappi Stanger mill’s activities. The SASS5 was similar with the Mvoti River sites having slightly higher scores, placing it in either a B or C class while most of the tributaries had a Class D. The community structure in the tributaries responded to nutrients and chlorides and these variables reduced the numbers of sensitive species and allowed hardy taxa to flourish. The results of the MIRAI index provided the same categories as identified by the SASS5 index. The fish community structure showed the same spatial differences identified in the macroinvertebrate communities, with the tributary sites containing different community structures than those found in the Mvoti River. The Mvoti River fish community is in a modified state with the majority of fish sampled being tolerant with very few sensitive species present. The FAII scores for the Mvoti River was a Category C, while the tributary sites scored a Category D or lower. The fish community is affected by the poor water quality, habitat and flow modifications in the lower Mvoti River. The FRAI index provided the lower Mvoti River and its tributaries with the same categories as identified with FAII. Overall, the Mvoti River is in a seriously degraded state with even the reference site being subjected to impacts that could potentially be harmful to the ecosystem. The ecological state of the river decreases as it moves past Stanger and is subjected to the impacts from the Nchaweni and Mbozambo rivers in the form of nutrients and salinity concentrations. The impacts on the Mvoti River have a multitude of different sources and if the aquatic ecosystem is to improve, only a collective effort will be of any value.
Prof. V. Wepener

The Isipingo River and Estuary system located in the province of KwaZulu-Natal, although relatively small, has been subjected to the impacts of intense development within the catchment. The catchment is characterized by informal and formal dwellings and intensive industrial activities in the lower catchment and in close proximity to the estuary. Significant modifications to the hydrology of the system has occurred as a result of the diversion of the Umlazi River (which previously merged with the Isipingo River at the estuary mouth), canalization of the lower portion of the Isipingo River flowing through the Prospecton Industrial Area and the development of the Diversion Works system (to regulate flows to the lower Isipingo River), removal of riparian vegetation, encroachment onto the river and estuarine floodplain, and land reclamation through the infilling of wetlands all of which has culminated in a deterioration of the functionality and health of this system. The main aspects of this study focus on water and sediment quality, material flux between the estuary and the sea and the management of the system. The water quality variables covered as part of this dissertation include physical, chemical (nutrients and heavy metals) and microbiological (faecal coliforms) components. The water quality of the river and estuary presents a risk to human health and the concentrations of heavy metals generally exceeded the South African Water Quality Guidelines that prescribes target levels, implying potential negative impacts to aquatic health. The Isipingo system exhibited signs of eutrophication with phosphorus concentrations in excess of the target level to prevent eutrophication. Excessive water hyacinth growth was visible during most of the field surveys. A geochemical assessment of heavy metals in surface sediments of the river and estuary was also undertaken. The results indicate that although the presence of several metals were undetectable at certain river sites, the concentrations of those heavy metals present increased in a longitudinal direction from the river to the estuary. The contamination factor analysis for all metals indicated low contamination except for selenium. The riverine sites yielded moderate enrichment by some heavy metals in both seasons. The degree of contamination for each site with the exclusion of selenium was low. The inclusion of selenium in the assessment raises the estuary to a considerably contaminated status in the wet season. The analysis suggests that the enrichment of the system by selenium is due to an anthropogenic source. The Geo-accumulation Index places the sediment in an overall unpolluted category. The study conducted on the net exchange of heavy metals and nutrients between the Isipingo Estuary and the adjacent marine environment (i.e. Indian Ocean) demonstrates that significant quantities of the suite of heavy metal analysed are exported on an annual basis while copper, nickel and zinc are imported into the estuary. Significant quantities of ammonia and nitrates are exported annually from the Isipingo Estuary while a net annual import of phosphorous is observed. The variation in the quantity and concentration of nutrients exported during the seasons may be attributed to anthropogenic source. The import of nutrients from marine sources can accumulate in the estuary and contribute to eutrophication of the Isipingo Estuary. This study also demonstrated that although the ebb flow has a much longer duration than the flood flow, the flood velocities observed were generally greater than the ebb velocities. The final part of this research identifies other environmental problems experienced in the Isipingo catchment. The following issues are identified as directly or indirectly contributing to stress and deterioration of the water quality of the Isipingo system: reduction in water quantity, poor water quality, moderate enriched sediment, loss of habitat integrity, species diversity and invasive alien vegetation and uncontrolled solid waste. A Water Quality Management Plan including management objectives, strategies and action plans to address the direct and indirect factors influencing the system are proposed. A water quality monitoring program is also proposed for the Isipingo River and Estuary. The adoption and execution of the water quality management plan and monitoring program will assist in the prevention of further degradation of the system and will be a stepping stone toward improvement.
Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2013.

The inefficiency of South Africa’s previous legislation that regulated the operation and closure of mining activities, has resulted in too many mines being abandoned without adequate rehabilitation (Naidoo, 2017). Therefore, currently, the State has taken a responsibility to identify all these abandoned mines, assess and rehabilitate them in order to mitigate their environmental impacts, mainly the acid mine drainage (AMD) impacts. A defunct colliery in Glencoe, which ceased its operation in 1973, is one of the mines that was identified as an abandoned mine. This mine is located on the upper Wasbank catchment area in KwaZulu-Natal and study was conducted in order to determine whether this defunct colliery has any negative effects on the water quality of this catchment area. A zone above the mine shaft of the defunct colliery was identified and considered as a pristine zone, which was then used as a reference zone, and three more zones were identified, adjacent and below the mine shaft, named sampling zone B, C and D in order to assess the trend of water quality indicators and therefore to determine whether the defunct colliery is contaminating this catchment area or not. Each zone had distinct sampling points within the zone, ranging from one to four sample points per zone. Water quality of this catchment area was tested on a monthly basis during the wet season (December 2017 to March 2018) and the dry season (April 2018 to July 2018). The parameters that were tested in the field were pH, electrical conductivity, and temperature. Furthermore, once a season, water samples were collected from each sampling point in order to determine the concentration of dissolved ions. All results were analyzed against the results of the reference zone and furthermore, against the South African Water Quality Guidelines for livestock farming and domestic use. The conclusion drawn from these results is that this catchment area is altered by mine drainage from the defunct colliery. However, it seems that mitigation measures designed by previous miners are effectively treating the AMD generated which has resulted in the neutralization of this acidity such that any decant from old mine works into the Wasbank River is slightly alkaline. Furthermore, based on SAWQG, the study concluded that this catchment is within the water quality standards for livestock farming and domestic use. However, there are minor restrictions to certain specific uses because of its salty state. Subsequent to the findings of this study, the defunct colliery can be considered as low priority of future rehabilitation efforts due to the state of water quality of the surrounding drainage
MT 2019

Submitted in partial fulfillment for the Degree of Master of Applied Sciences in Biotechnology, Durban University of Technology, Durban, South Africa, 2017.
The study aims to assess and compare the concentration of microbial contaminants, their sources and distribution in surface water and sediment, and to determine the impact of seasonal variations and corresponding risks of faecal contamination using conventional and molecular methods. Historical data analysis was conducted using E. coli values from the eThekwini Water and Sanitation (EWS) department for 66 months (2009-2014). E. coli and Enterococci were analysed in surface water and sediment samples using the mFC/ spread plate and Colilert-18 (IDEXX) methods. The impact of seasonal variations was assessed using E. coli and Enterococci data collected during rainfall and no rainfall events, using an auto-sampler and sediment trap in parallel. Conventional standard membrane filtration methods using mFC agar, Slanetz & Bartley/ Bile Esculin and Brilliance E. coli selective agar were compared to the enzymatic Colilert-18 and Enterolert (IDEXX) test methods along the Isipingo and Palmiet Rivers. In addition, comparison of the analytical performance of droplet digital PCR (ddPCR) and qPCR for the detection of Salmonella targeting ttr gene in river sediment samples collected from the four sites of the Palmiet River in Durban, South Africa was done. In order to assess the public health risk associated with exposure of men, women and children to microbial pathogens in polluted surface water during recreational activities, the QMRA tool was employed in relation to the risk exposure to pathogenic E. coli, Campylobacter, Salmonella and Shigella. Also, the risk associated with crop irrigation (on farmers) as well as the consumption of crops irrigated with surface water from the Isipingo river was determined. Analysis of the historical data gave a baseline of the two rivers of interest, thus helps understand the current situation of the rivers enabling researchers to pick up potential gaps. In this study after the analysis of the historical data it was evident that at the Palmiet river, microbial analysis must be conducted around the QRI settlements which is a major pollution source. Also, from this study it was found that sampling points situated close to wastewater treatment plants, pump stations or informal settlements were of major concern, thus were considered for the study. It was found that sediment exhibited higher microbial concentrations than surface water, which was observed in both rivers. Also, rainfall had a significant impact on microbial variability. Higher microbial concentrations (indicator organisms) were observed in surface water after a heavy rainfall as appose to when there was no rainfall. This was due to contamination that is washed off into the river and sediment resuspension. Methodology comparison revealed that Colilert-18 and Brilliance E. coli were more selective compared to mFC agar. Brilliance E. coli /Coliform agar was comparable with Colilert-18 IDEXX, which was also observed with Slanetz & Bartley and Enterolert IDEXX. However, when mFC agar was compared with Colilert-18 IDEXX, significant difference was observed. In comparison of two Molecular methods, ddPCR were found to be fully amenable for the quantification of Salmonella and offer robust, accurate, high-throughput, affordable and more sensitive quantitation than qPCR in complex environmental samples like sediments. Quantitative Microbial Risk Assessment (QMRA) relating to recreational and occupational exposure showed that children were at the highest risk of getting infected. Also, it was observed that the probability of infection upon exposure to surface water from the Isipingo and Palmiet rivers was significantly high, hence exceeded the WHO guidelines values. Risk assessment on crops revealed that pathogenic bacteria may pose a risk to the consumer, however, a 9-log reduction may be achieved according to the WHO multi-barrier approach which involves proper washing and proper cooking of the crop before ingestion. Overall the sampling points that had the highest pollution level and constantly exceeded the WHO and DWAF guidelines at the Isipingo river were the points situated and named “Next to the WWTP”, and “Downstream of QRI” at the Palmiet River.
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Water, a fundamental and irreplaceable resource, is an all-pervasive issue that underpins the social fabric of every society. Rapid population growth and expansion of human activities increases the amount of waste and pollution generated and many local authorities are encountering serious water pollution problems, often concentrated in the lower reaches of catchments and adjacent coastal areas. This problem is predominantly acute in urbanized catchment areas, where waste is concentrated into localized areas, and the authorities are constantly under pressure to provide adequate management and mitigation measures. The Palmiet River system, located in the northern fringe of the city of Durban and draining the highly industrialized Pinetown region in KwaZulu-Natal, South Africa, illustrates a system that has been altered due to human impacts, particularly in its headwaters where the industrial sites are located and, in the lower catchment where a densely populated informal settlement occurs. A range of chemical and biotic indicators were monitored seasonally and these confirm the influence of the aforementioned human impacts on the quality of the Palmiet River system. Results from the present study were compared with studies conducted over a period of two decades and clearly demonstrate a pattern of increasing pollution loads for the upper and lower parts of the catchment. This study confirms that the Palmiet River is severely degraded in its lower reaches whilst the middle reaches of the catchment where a nature reserve is located is still in a fairly pristine condition. Additionally, the Palmiet River issues discussed in this thesis have direct impacts on the estuarine and adjacent marine ecosystems.
Thesis (M.A.)-University of KwaZulu-Natal, Durban,2005.

One of the main challenges facing the potable water production industry is deterioration of the quality of raw water. Drinking water that does not meet quality standards is unfit for consumption. Yet, this quality is a function of various factors, key among them being quality of the raw water from which it is processed. This is because costs related to potable water treatment are related to the nature of raw water pollutants and the degree of pollution. Additionally, survival of aquatic species depends on self-purification of the water bodies through attenuation of pollutants, therefore, if this process is not efficient it might result in dwindling of the aquatic life. Hence, this chapter presents spatial and temporal water quality trends along uMngeni Basin, a critical raw water source for KwaZulu-Natal Province, in South Africa. As at 2014 the basin served about 3.8 million people with potable water. Results from this study are discussed in relation to uMngeni River’s health status and fitness for production of potable water treatment. Time-series and box plots of 11 water quality variables that were monitored at six stations over a period of eight years (2005 to 2012), were drawn and analysed. The Mann Kendall Trend Test and the Sen’s Slope Estimator were employed to test and quantify the magnitude of the quality trends, respectively. Findings showed that raw water (untreated) along uMngeni River was unfit for drinking purposes mainly because of high levels of Escherichia coli. However, the observed monthly average dissolved oxygen of 7 mg/L, that was observed on all stations, suggests that the raw water still met acceptable guidelines for freshwater ecosystems. It was noted that algae and turbidity levels peaked during the wet season (November to April), and these values directly relate to chlorine and polymer dosages during potable water treatment.