Journal articles on the topic 'Weighing instruments industry'

In the experimental calibration processes, several error of measure can be obtained. Usually, these values are called as outliers. The outliers impact in the instruments’ metrological reliability and, consequently, in the industrial processes’ quality control. In specific terms, for mass metrology, the outliers are extremely critic, due to sensibility of technology used for this industry, i.e.: non-automatic weighing instruments (scales). The EURAMET/cg-18/v.02 is the international standard that defined procedure and tests for to assessment the metrological reliability of scales, however, a statistical treatment for outliers is not defined. In order to propose robust alternatives for outliers analyses, this work to evaluate parametric techniques for the elimination of outliers in the calibration of a Bernalo non-automatic weighing instruments (Capacity: 30.000 kg; Resolution: 0.001 kg). Three methods were applied in the analysis of experimental data: Dixon, Grubbs and Chauvenet. The results confirmed a reduction in the expanded uncertainty associated to mass measurement (k=2) up to: 17.6% (Dixon); 19.9% (Grubbs) and 35.2% (Chauvenet). According to the results and considering the contribution of work to applied mass metrology, the authors propose the inclusion of analyzes and evaluation of outliers in the EURAMET/cg-18/v.02. Thus, it is expected an increase in metrological reliability in mass measurement processes at the scientific and industrial sector.

AbstrakPenentuan ketulenan jongkong emas dengan kaedah ujian tanpa musnah adalah perkara yang paling mencabar dalam industri emas terutama jika jongkong emas dipalsukan dengan cara meletakkan tungsten di dalamnya. Instrumen yang sedia ada mempunyai keterbatasan untuk menentukan ketulenan jongkong emas dan sukar untuk mengesan tungsten di dalamnya kerana tungsten mempunyai ketumpatan yang hampir sama dengan emas. Kajian ini mengemukakan keterbatasan instrumen kaedah ujian tanpa musnah seperti densimeter, alat timbangan, sinar-x pendarfluor dan ultrasonik. Dalam kajian ini menemui kesukaran untuk mengenal pasti emas palsu menggunakan instrumen kaedah ujian tanpa musnah seperti ultrasonik dan x-ray fluorescence (XRF) kerana instrumen ini mempunyai batasan. Setakat ini tiada lagi kajian yang berkaitan pemalsuan jongkong emas kerana harganya yang mahal untuk dijadikan sebagai sampel kajian. Masalah jongkong emas palsu dapat diselesaikan jika ketepatan instrumen yang digunakan untuk mengukur ketumpatan dapat ditingkatkan lagi sehingga 0.01 g/ml. Ini kerana perbezaan antara ketumpatan emas dan tungsten adalah 0.05 g/ml. Pemalsuan emas dengan mencampurkan tungsten akan dapat dikesan jika ada instrumen yang mempunyai ketepatan sekurang-kurangnya 0.01 g/ml. Manakala instrumen lain seperti XRF digunakan untuk mengesahkan ketulenan emas di bahagian permukaan sahaja. Oleh itu, bagi pengujian yang lebih menyeluruh masalah jongkong emas palsu ini perlu diselesaikan dengan menggabungkan kedua-dua kaedah ini. Abstract Determination of the gold bar purity by non-destructive method remains one of the most challenging in gold industry especially when the tungsten is inside the gold bar. The existing instruments have limitations in their accuracy to determine the purity of gold when tungsten also inside the gold bar since both tungsten and gold have almost similar densities. This study presents the limitations of the non-destructive test method instruments such as densimeter, weighing balance, x-ray fluorescence (XRF) and ultrasonic in determining the purity of gold. In this study found the difficulty in identifying counterfeit gold using non-destructive test method instruments such as ultrasonic and XRF due to some limitations. So far, there are no studies related to the counterfeiting of gold bars due to its expensive price to be used as a study sample. The problem of counterfeit gold bars can be solved if the accuracy of the instrument used to measure the density can be further increased up to 0.01 g/ml. This is because the difference between the density of gold and tungsten is 0.05 g/ml. Gold counterfeiting by mixing tungsten will be detectable if there is an instrument that has an accuracy of at least 0.01 g/ml. While other instruments such as XRF will be used to verify the purity of gold on the surface only. Therefore, for more thorough testing the problem of fake gold ingots needs to be solved by combining these two methods.

<p>Partnerships are instruments to overcome the inability of individual smallholders to solve the development problems associated with agricultural sectors and commodity chains. While vigorously advocated, a sustainable partnership is rare in the Indonesian cassava production and industrial sector. Knowledge of partnership from the sector is also limited. This study explores the reasons for previous partnerships in the cassava production and industrial sector discontinued and examine what factors determine farmers' willingness to partake in partnerships. The study administered a systematic random sampling to survey for 140 cassava farmers in Central and East Lampung Districts from November 2018 to April 2019. Farmers were interviewed and given a structural questionnaire. The quantitative data were analyzed by heckprobit regression. The results of the study show that the partnership ends because the two parties do not have the same vision for working together. Various variables (barriers to selling to non-industries, low prices, inaccurate weighing scales, high transaction costs, and discounted prices.) indicate the complexity of farmers' considerations for joining the partnership. This research impliesthat it is difficult to create partnerships in the future if farmers and industry have different visions and priorities.</p>

Abstract. Sulfur dioxide (SO2) measurements from the Ozone Monitoring Instrument (OMI), Ozone Mapping and Profiler Suite (OMPS), and TROPOspheric Monitoring Instrument (TROPOMI) satellite spectrometers were used to update and extend the previously developed global catalogue of large SO2 emission sources. This version 2 of the global catalogue covers the period of 2005–2021 and includes a total of 759 continuously emitting point sources releasing from about 10 kt yr−1 to more than 4000 kt yr−1 of SO2, that have been identified and grouped by country and primary source origin: volcanoes (106 sources); power plants (477); smelters (74); and sources related to the oil and gas industry (102). There are several major improvements compared to the original catalogue: it combines emissions estimates from three satellite instruments instead of just OMI, uses a new version 2 of the OMI and OMPS SO2 dataset, and updated consistent site-specific air mass factors (AMFs) are used to calculate SO2 vertical column densities (VCDs). The newest TROPOMI SO2 data processed with the Covariance-Based Retrieval Algorithm (COBRA), used in the catalogue, can detect sources with emissions as low as 8 kt yr−1 (in 2018–2021) compared to the 30 kt yr−1 limit for OMI. In general, there is an overall agreement within ±12 % in total emissions estimated from the three satellite instruments for large regions. For individual emission sources, the spread is larger: the annual emissions estimated from OMI and TROPOMI agree within ±13 % in 50 % of cases and within ±28 % in 90 % of cases. The version 2 catalogue emissions were calculated as a weighted average of emission estimates from the three satellite instruments using an inverse-variance weighting method. OMI, OMPS, and TROPOMI data contribute 7 %, 5 %, and 88 % to the average, respectively, for small (<30 kt yr−1) sources and 33 %, 20 %, and 47 %, respectively, for large (>300 kt yr−1) sources. The catalogue data show an approximate 50 % decline in global SO2 emissions between 2005 and 2021, although emissions were relatively stable during the last 3 years. The version 2 of the global catalogue has been posted at the NASA global SO2 monitoring website (https://doi.org/10.5067/MEASURES/SO2/DATA406, Fioletov et al., 2022).

The Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) is a cooperative effort between NASA and Japan's Ministry of Economy Trade and Industry (METI), with the collaboration of scientific and industry organizations in both countries. The ASTER instrument provides a more robust remote sensing imaging capability when compared to the older Landsat Thematic Mapper. This paper deals with the accuracy assessment of elevation data obtained using ASTER from each of the eleven (11) selected extrapolation/interpolation algorithms: Inverse Distance Weighting, Natural Neighbor, Spline Regular, Spline Tension, Universal Kriging, Empirical Bayesian Kriging, Topo to Raster, global (trend surface), local polynomial, kernel interpolation with barriers and radial basis functions in Digital Elevation Model (DEM) surface creation. The data were compared with reference to ground control points of differential GPS measurements in the study area. The error statistics were generated between DGPS measurements and Extracted elevation data from each selected interpolation method. It was observed that Spline Regular Interpolation shown the best overall accuracy of ±11.520m when elevation data extracted from Inverse distance weighting, Natural Neighbour, Spline T, Topo to Raster, Universal Kriging, Empirical Bayesian kriging, Global polynomial interpolation (GPI), local polynomial interpolation (LPI), Radial basis function and Kernel interpolation of ±15.170, ±14.340, ±12.336, ±13.551, ±14.707, ±13.711, ±15.363, ±13.964, ±13.590 and ±15.376 respectively when compared with elevation values from GPS method. The study recommends capacity building in the form of workshop, training, and flexible integration of point elevation data to DEM.

The Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) is a cooperative effort between NASA and Japan's Ministry of Economy Trade and Industry (METI), with the collaboration of scientific and industry organizations in both countries. The ASTER instrument provides a more robust remote sensing imaging capability when compared to the older Landsat Thematic Mapper. This paper deals with the accuracy assessment of elevation data obtained using ASTER from each of the eleven (11) selected extrapolation/interpolation algorithms: Inverse Distance Weighting, Natural Neighbor, Spline Regular, Spline Tension, Universal Kriging, Empirical Bayesian Kriging, Topo to Raster, global (trend surface), local polynomial, kernel interpolation with barriers and radial basis functions in Digital Elevation Model (DEM) surface creation. The data were compared with reference to ground control points of differential GPS measurements in the study area. The error statistics were generated between DGPS measurements and Extracted elevation data from each selected interpolation method. It was observed that Spline Regular Interpolation shown the best overall accuracy of ±11.520m when elevation data extracted from Inverse distance weighting, Natural Neighbour, Spline T, Topo to Raster, Universal Kriging, Empirical Bayesian kriging, Global polynomial interpolation (GPI), local polynomial interpolation (LPI), Radial basis function and Kernel interpolation of ±15.170, ±14.340, ±12.336, ±13.551, ±14.707, ±13.711, ±15.363, ±13.964, ±13.590 and ±15.376 respectively when compared with elevation values from GPS method. The study recommends capacity building in the form of workshop, training, and flexible integration of point elevation data to DEM.

PurposeWith the increasing attention acquired from researchers and practitioners in Architecture, Engineering and Construction (AEC) industry, building information modeling (BIM) has fundamentally changed the approach we design, construct and delivery, as well as operate and maintenance of buildings and civil infrastructures. This study tries to provide an innovative perspective on BIM research. This study aims to analyze the necessity and feasibility of BIM user satisfaction research and define what BIM user satisfaction is, and then to develop a quantitative method for the measurement of BIM user satisfaction.Design/methodology/approachAs it is indicated in the content, BIM user satisfaction is measured by the sum of the user's weighted reactions to a set of factors. To be specific, the entropy method was adopted to calculate the “weighting” of the factors, and the triangular fuzzy number (TFN) method was selected to compute the “scoring” of the factors. Through the literature review, methodology and tool development, as well as case study and discussions, this paper was generated sequentially.FindingsThis study found that the proposed tool for the measurement of BIM success is valid and reliable; it formerly translated the conceptual definition of BIM user satisfaction into an accurate measurement instrument. It also indicated that many factors are affecting the BIM users' satisfaction, and each of the factors inherited various importance and score, and the findings are expected to improve the performance and effectiveness of BIM management.Originality/valueThrough the translation of the conceptual BIM user satisfaction into a valid quantitative measurement instrument, this research provides an excellent framework for the management of BIM from the user's perspective, and it could help to stimulate user's acceptance of BIM in the AEC industry in future.

The purpose of the article is to develop and approbate a methodical approach to analyzing the financial security of priority sectors of the national economy. The article emphasizes the relevance of considering the issues of economic and, in particular, financial security at the level of the sectoral economic system. The authors’ own methodology of analysis is developed and the weighting factors of indicators of financial security of agriculture, industry, construction and transport, warehousing, postal and courier activities of Ukraine are evaluated. The structure of financial security of priority sectors of the national economy, i. e., the investment, credit, debt, monetary, production and economic, system-structural components is determined and the weighting factors of indicators are evaluated according to these security components. This approach made it possible to identify the indicators with a lesser extent along with the indicators with a greater extent of an impact they have on the general condition of subindexes, and therefore determine the overall level of financial-economic security of priority sectors of the national economy of Ukraine. The integral values of financial security of priority sectors of the national economy of Ukraine for 2010-2020 were computed. The results of the assessment allowed to identify, firstly, the state of financial security of the analyzed industries; secondly, the dynamic characteristics of changes in the level of financial security of priority sectors of the national economy; thirdly, the measure of differentiation in the state of financial security among the analyzed types of economic activity; fourthly, to form conclusions regarding the factors influencing the parameters of financial security of priority sectors of the national economy of Ukraine; fifthly, to determine the strategic directions and develop a set of the State policy instruments focused on strengthening financial security, taking into account the existing specifics for agriculture, industry, construction and transport, warehousing, postal and courier activities of Ukraine. Prospects for further scientific research in the analyzed sphere relate to the formation of recommendations regarding the linking of the results of the accomplished analysis with the formation of actions in the field of strengthening the financial-economic security of priority sectors of the Ukrainian economy.

Abstract The stakeholders and companies in the real estate industry are facing new challenges. They must react to megatrends such as climate change, resource depletion, shift in social values, demographic change and digitalization and at the same time comply with their responsibility to society and environment. In order to contribute to sustainable development and thereby ensure the future viability of the company, it is necessary to consider the social, environmental and economic impact of any business activities and decisions. Referring to the German market, a discussion will be based on the example of housing companies to show how sustainability aspects can be integrated into the instrument of portfolio analysis. The interactions between sustainability aspects resulting of the external business environment, the respective location characteristics, the building performance and the rentability and marketability as a success factor will be discussed. In this context, it will be explained how to react dynamically to a changing environment by using adaptable weighting factors and to local location and market requirements. As a conclusion, it will be discussed how the concerns of society, the cities (housing districts) and the tenants can be taken into account in addition to the economic interests of the housing companies.

Research background: The aim of this paper is to synthesize and analyze existing evidence on artificial intelligence-based decision-making algorithms, industrial big data, and Internet of Things sensing networks in digital twin-driven smart manufacturing. Purpose of the article: Using and replicating data from Altair, Catapult, Deloitte, DHL, GAVS, PwC, and ZDNet we performed analyses and made estimates regarding cyber-physical system-based real-time monitoring, product decision-making information systems, and artificial intelligence data-driven Internet of Things systems in digital twin-based cyber-physical production systems. Methods: From the completed surveys, we calculated descriptive statistics of compiled data when appropriate. The data was weighted in a multistep process that accounts for multiple stages of sampling and nonresponse that occur at different points in the survey process. The precision of the online polls was measured using a Bayesian credibility interval. To ensure high-quality data, data quality checks were performed to identify any respondents showing clear patterns of satisficing. Test data was populated and analyzed in SPSS to ensure the logic and randomizations were working as intended before launching the survey. An Internet-based survey software program was utilized for the delivery and collection of responses. The sample weighting was accomplished using an iterative proportional fitting process that simultaneously balanced the distributions of all variables. The interviews were conducted online and data were weighted by five variables (age, race/ethnicity, gender, education, and geographic region) using the Census Bureau’s American Community Survey to reflect reliably and accurately the demographic composition of the United States. Confirmatory factor analysis was employed to test for the reliability and validity of measurement instruments. Findings & Value added: The way Internet of Things-based decision support systems, artificial intelligence-driven big data analytics, and robotic wireless sensor networks configure digital twin-driven smart manufacturing and cyber-physical production systems in sustainable Industry 4.0.

Abstract. Carbon monoxide (CO) is an important atmospheric constituent affecting air quality, and methane (CH4) is the second most important greenhouse gas contributing to human-induced climate change. Detailed and continuous observations of these gases are necessary to better assess their impact on climate and atmospheric pollution. While surface and airborne measurements are able to accurately determine atmospheric abundances on local scales, global coverage can only be achieved using satellite instruments. The TROPOspheric Monitoring Instrument (TROPOMI) onboard the Sentinel-5 Precursor satellite, which was successfully launched in October 2017, is a spaceborne nadir-viewing imaging spectrometer measuring solar radiation reflected by the Earth in a push-broom configuration. It has a wide swath on the terrestrial surface and covers wavelength bands between the ultraviolet (UV) and the shortwave infrared (SWIR), combining a high spatial resolution with daily global coverage. These characteristics enable the determination of both gases with an unprecedented level of detail on a global scale, introducing new areas of application. Abundances of the atmospheric column-averaged dry air mole fractions XCO and XCH4 are simultaneously retrieved from TROPOMI's radiance measurements in the 2.3 µm spectral range of the SWIR part of the solar spectrum using the scientific retrieval algorithm Weighting Function Modified Differential Optical Absorption Spectroscopy (WFM-DOAS). This algorithm is intended to be used with the operational algorithms for mutual verification and to provide new geophysical insights. We introduce the algorithm in detail, including expected error characteristics based on synthetic data, a machine-learning-based quality filter, and a shallow learning calibration procedure applied in the post-processing of the XCH4 data. The quality of the results based on real TROPOMI data is assessed by validation with ground-based Fourier transform spectrometer (FTS) measurements providing realistic error estimates of the satellite data: the XCO data set is characterised by a random error of 5.1 ppb (5.8 %) and a systematic error of 1.9 ppb (2.1 %); the XCH4 data set exhibits a random error of 14.0 ppb (0.8 %) and a systematic error of 4.3 ppb (0.2 %). The natural XCO and XCH4 variations are well-captured by the satellite retrievals, which is demonstrated by a high correlation with the validation data (R=0.97 for XCO and R=0.91 for XCH4 based on daily averages). We also present selected results from the mission start until the end of 2018, including a first comparison to the operational products and examples of the detection of emission sources in a single satellite overpass, such as CO emissions from the steel industry and CH4 emissions from the energy sector, which potentially allows for the advance of emission monitoring and air quality assessments to an entirely new level.

From many kinds of investment in Indonesian capital markets, a mutual fund is one of the instrument to be discussed. Within a short time, the mutual fund gives a significant influence to the Indonesian capital markets. A mutual fund is one of the alternatives for the investors, especially for small investors or for the investor that does not want to meet high risk. In the growing of Indonesian capital markets, the net asset value (NAV) of the mutual fund should be increasing, and the increase of the NAV proves it. The sharia equity fund is no different. As an alternative investment, the sharia mutual fund in Indonesia is growing; however, it does not seem attractive to the investor, reflected by the small weighting from the mutual fund industry. The reason why investors are reluctant to invest in sharia equity fund is the volatility, compared to the conventional equity fund. To guide investors and to attract investment in sharia equity funds, some indicators can be used, such as PER, PBR and PSR. This study will use these indicators as the independent variables: PER (X1), PBR (X2) and PSR (X3) on the Net Asset Value (NAV) as the dependent variable (Y) in sharia equity fund using the Jakarta Islamic Index (JII) data. In this research, the researcher uses a quantitative method using secondary data. The results show that there is a significant influence of PER, PBV and PSR on NAV in sharia equity funds. As a consequence, PER, PBR and PSR can guide investors’ decisions to invest in sharia mutual fund. Keywords: PER, PBR, PSR, NAV, sharia mutual fund

Purpose The purpose of this study, a case study, is to present the uniqueness of the only UNESCO-awarded tea consumption tradition worldwide and its implications on the tourism industry by analyzing and weighting initializing effects of tea on the regional economy, as well tea tourism-related cultural and social rootings. Design/methodology/approach Between 20 July 2020 and 20 August 2020, qualitative interviews have been conducted with regional experts, as well as relevant statistics have been evaluated, cultural and social effects have been analysed and weighted, following two main research questions: can “Tea”, along with its history and tradition, be used effectively for initializing tourism and destination brand management? What kind of instruments are useful for reinforcing authentic tea-oriented destination marketing? In addition, the European Tea Speciality Association (ESTA) supported this research project also with another qualitative interview. Furthermore, two workshops of the Working Group on International Tea Tourism have been used to discuss and reflect the impacts of tea museums and tea consuming traditions as well as the European Tea Research Circle (ETRC). Findings Tea and its culture is a strong motive for initializing tourism, as it is linked to cosy ceremonies and social gathering. The brand awareness of the destination “East Frisia” as the “tea destination” of Germany is powerful, and the “East Frisian Tea Ceremony” became a regional social anchor and element of loyalty for tourists and citizens. It is useful and profitable to develop more tea-related authentic evens and products to use this UNESCO award and the positive image of tea ceremonies to strengthen the regional economy. Research limitations/implications As the UNESCO award was awarded in 2016, any impacts could only be evaluated for a short period of time. Practical implications The specific tea culture in East Frisia has the potential for establishing this region as the most unique region for Tea Tourism in Central Europe. This should be evaluated and developed within additional measurements and programmes. The aim should be a plan for additional mapping and tea-related events and attractions to use this unique heritage for developing sustainable tourism and strengthening the regional hospitality infrastructure. Social implications The East Frisian tea culture is empowering people and functions as a harmonic link among the inhabitants of a structurally underdeveloped region. Thus, this habit is a key factor for the stability and pride of the inhabitants, involving citizens and keeping traditions alive. Originality/value The combination of a cultural heritage award and a habit for celebrating the consumption of a hot beverage is unique worldwide. Therefore, this analysis is a valuable support for the transfer of applicable knowledge from academia to the industry as well as the first research project about the link between tea consuming heritage and tourism in Europe.

ANTI-DIABETIC EFFECTS OF ETHANOL LEAF EXTRACT OF ONIONS (Allium cepa) ON ALLOXAN-INDUCED DIABETIC WISTAR ALBINO RATS Choji Solomon S. Department of Biochemistry, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos. P.M. B 2012, Jos. Nigeria. Chojisolomon@gmail.com +2347065752410 Damla Faith U. Department of Biochemistry, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos. P.M. B 2012, Jos. Nigeria Barde Larry A. Department of Biochemistry, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos. P.M. B 2012, Jos. Nigeria Zakka Riyang. Department of Food Science and Technology, Faculty of Agricultural Science, Federal University Wukari. P.M.B 1020 Adegbite Adeshola. Ladoke Akintola University of Technology. Ogbomoso. Oyo State. Abstract. Diabetes is a chronic disease characterised by high blood glucose level and abnormal metabolism of carbohydrates, protein and fat. The condition is characterised by persistent hyperglycaemia. Allium cepa leaf is a functional food used in traditional medicine for the treatment of diabetes mellitus. The use of plants especially vegetable as antidiabetic remedies have added interest of joining two basic diabetes mellitus control factors: food and medication. The ethanol extract of Allium cepa leaf was investigated for antidiabetic effects using alloxan- induced diabetic wistar albino rats. Wistar albino rats were randomly divided into six groups; Group A rats were non-diabetic control. Diabetes was induced in groups B, C, D, E and F by single intraperitoneal injection of alloxan (150mg/kg body weight). Group B were not treated and served as negative control group. Group C were treated with glibenclamide (5mg/kg body weight), thus served as postive control group. Groups D, E and F were treated with 200, 300 and 400 mg/kg body weight of the extract respectively for a period of two weeks through intraperitoneal route. The effect of treatment with the doses of the extract and standard drug were studied on blood glucose level, total serum cholesterol and body weight. Allium Cepa extract produced a dose- dependent significant reduction in the blood glucose level when compared with that of the control group. Significant total serum cholesterol reduction was observed at 300 and 400mg/kg. An observed decrease in body weight of the negative control group was recorded and significant increase for all other groups. The findings from this study indicate that the crude extract of Allium cepa leaf caused a significant hypoglycaemic and hypocholesterolemic activity in alloxan-induced diabetic rats thus, validates its use in ethno – medicine for the control of diabetes mellitus. KEY WORDS: Diabetes mellitus, Allium cepa, Alloxan, Blood glucose, Cholesterol Glibenclamide. 1.0 INTRODUCTION. Diabetes Mellitus (DM) is a group of metabolic disorders associated with disturbances in the metabolism of fuel molecules due to absolute deficiency of insulin, insufficient insulin secretion and / or its secretion [1]. It is a disorder that affects the body’s ability to make or use insulin. Insulin is a hormone produced in the pancreas that helps transport glucose (blood sugar) from the bloodstream into the cells so they can break it down and use it for fuel. People cannot live without insulin [2]. It is also a widespread endocrine disorder that is associated with considerable morbidity and mortality and is found in all population throughout the world [3] Despite the presence of anti-diabetic drugs in the pharmaceutical market, the treatment of diabetes with medicinal plants is often successful. Herbal medicine and plant components with insignificant toxicity and less or no side effect are notable therapeutic options for the treatment of this disease around the world [4]. The most common herbal active ingredients used in treating diabetes are flavonoids, tannins, phenols and alkaloids [5]. The existence of these compounds implies the importance of the anti-diabetic properties of these plants [4]. Allium cepa is one of the recognised medicinal plants known to possess several medicinal properties including lowering of blood pressure, antiseptic, hypoglycaemic and hypocholesterolemic activity [6]. In the rural communities, many people depend solely on medicinal plants for the treatment of diabetes due to its easy accessibility, affordability and availability even when the efficacy of the herbal remedies has not been established [6]. Dietary therapy is unarguably the best treatment for diabetes. The diabetic diet should be carefully monitored to minimize the load placed on the blood glucose regulating mechanism. The use of plants, especially vegetables, by the population as antidiabetic remedies has added interest of joining two basic diabetes mellitus control factors: food and medication [7]. This research is thus geared towards finding a medicinal plant that will not only increase the energy content of diabetics but also lower glycaemic index properties for the management of diabetic pressures in our society. 2.0 MATERIALS AND METHODS. 2.1 Materials. 2.1.1 Chemicals and Reagents. Baker Ltd Dagenham, England, BDH Chemicals Ltd; Poole England, Sigma Chemicals, St Louis, USA, Emzor Pharmaceuticals Industry Ltd, Nigeria and Randox Laboratories. London, UK. 2.1.2 Plant The Allium cepa leaves used for the experiment was bought from Barkin Ladi Market, Plateau State, Nigeria. The plants were identified by Professor Pob Poppva in the Department of Botany, University of Jos, Plateau State. A voucher specimen was deposited in the herbarium unit of the department. 2.1.3 Experimental Animals. A total of thirty-six (36) adult male Wistar albino rats weighing 80 to 150g and twelve (12) mice were used for the experiment. The experimental animals were purchased from Chris Animal Farm, G.R.A. Awka. They were housed six (6) rats per cage at the experimental Animal House of Biochemistry Department, Nnamdi Azikiwe University, Awka, Anambra State. They were acclimatized for two weeks under standard laboratory conditions and were maintained on water and Guinea growers mash pellet (Vital Feed Grand Cereals Nigeria Ltd, Jos, Nigeria) that was obtained from Eke Market, Awka, Anambra State. 2.2 Methods 2.2.1 Preparation of ethanol leaf extract of Allium cepa . The leaves of Allium cepa were properly washed with distilled water and dried at room temperature for three weeks. The dried leaves were then pulverised using corona manual grinding machine. The powdered samples of Allium cepa was weighed and exactly 1475g was extracted in 5 litres of 80% ethanol for 24 hours with occasional stirring, sieved and filtered using filter paper (Whatman number 1). The filtrate was then concentrated using a rotary evaporator at 600C and appeared as a dark brown gel solid. The extracts were kept in a labelled glass container and stored in a refrigerator until when required for reconstitution and administration. 2.2.2 Phytochemical Screening of Secondary metabolites(Constituents) The qualitative phytochemical screening of the ethanol leaf extract of Allium cepa was carried out using standard procedures as outlined by [8], [9]. 2.2.3 Acute toxicity and Median Lethal Dose (LD50) test of ethanol leaf extract of Allium cepa. The median Lethal Dose (LD50) was determined using Wistar albino mice as described by the modified method of [10]. Test animals were divided into six (6) groups. The first 3 groups which contain 3 animals each were given 10mg/kg, 100mg/kg and 1000mg/kg body weight of the ethanol extract of Allium Cepa leaves. The Allium Cepa extract was administered orally and was monitored for 24 hours. The last 3 groups which contain one animal each per group were then given 1600mg/kg, 2900mg/kg and 5000mg/kg body weight of the ethanol extract of Allium Cepa leaves and were observed for 24 hours. 2.2.4 Induction of Diabetes. Alloxan was prepared and induced by adopting the method of [11]. All rats, except for the normal control group were intraperitoneally injected with 150mg/kg body weight of the prepared alloxan dissolved in normal saline solution. The blood glucose levels of the rats were checked before the administration of alloxan using one touch glucometer (Fine touch, USA) and test strips. The rats were then fasted for 16 hours, but with free access to water after which they received an intraperitoneal injection of alloxan 150mg/kg body weight. The rats were orally given 20ml each of 10% glucose solution after 2 hours to prevent hypoglycaemia. The animals were allowed free access to food and water after alloxan administration. After 48 hours of the alloxan administration, blood was collected orbito-rectally and their glucose levels were checked using one touch glucometer and test strips. Diabetes was confirmed to have been induced if the glucose level was observed to be far much higher than normal (above 140mg/dl). 2.2.5 Experimental Design This study was carried out on alloxan –induced diabetic rats for two (2) weeks. A total of thirty-six (36) Wistar albino rats were used for the experiment. The albino rats were randomly divided into six (6) groups with six (6) rats in each group. The extract and the reference drug were administered intraperitoneally to the animals. Group A – Normal (non-diabetic control) Group B – Diabetic (negative) control group Group C – Diabetic (positive) control – this group received 5mg/kg body weight of glibenclamide. Group D – This group received 200mg/kg body weight of the extract. Group E – This group received 300mg/kg body weight of the extract. Group F – This group received 400mg/kg body weight of the extract The weights of the animals were carefully monitored before the induction and throughout the duration of the experiment. 2.2.6 Biochemical Assay 2.2.6.1 Blood glucose level determination Determination of the blood glucose level was done by the glucose-oxidase principle [12] using the one touch instrument and results were reported as mg/dl [13]. 2.2.6.2 Determination of total serum cholesterol. The cholesterol of the serum was oxidised to tetraene derivative by ferric ions derived from ferric perchlorate using four different test tubes that were marked test, control, standard and blank. The absorbance was measured (using spectrophotometer) at 590nm wavelength and compared with that of a pure solution of cholesterol [14]

This paper presents, design, build, assemble and commission of an automatic Sorting machine by using Weighing mechanism. The popular measuring technique is weighing scales or weighing machines. Weighing machines are measuring instruments which are used to determine the weight or mass of an object. The electronic weight detection system is classified into two types: the strain/ force gauge load cell and the electromagnetic force system. Strain gauge load cells are the most common in industry. In this system, Weight of the object moving in the conveyor is measured .Then it is sorted based on its weight the measured. The weight of the product is feed to the controller. The standard weight of the product is set to the controller. When the weight is not equal to the preset value, the object is sorted in one side. If it meets the value, it is sorted to the other side. This is the basic mechanism used in the sorting machine by weight

One of the critical problems for ships utilization is the limited operative life which affect its efficiency. Therefore, ship owners tend to send these ships for recycling. On the one hand, ship recycling industry can absorb labour and become a source of state revenue. Nevertheless, these activities significantly affect the preservation of the marine environment if not appropriately managed. Marine waste pollution is an essential issue in global context. Regrettably, as a country with a massive shipping intensity and geographically a strategic location, Indonesia does not have a comprehensive national policy regarding recycling ships weighing for 500 GT or more. The government allows conventional ship recycling methods that are not environmentally friendly and sustainably, which contradicts Indonesia's commitment to supporting the Sustainable Development Goals (SDGs). Thus, Indonesia is vulnerable to claims regarding poor pollution management due to ship dismantling activities. The claim could be addressed to the state’s responsibility, which refered to international instruments such as conventions and guidelines in terms of preventing pollution to neighboring countries originating from ship breaking and dismantling activities that lack regulation and measures. This article argues the urgency to reform the current national ship recycling regime in the future to apply a greener method by alluding to international general principles, customary and jurisprudence.

Abstract Objective The transferability of the EU joint clinical assessment (JCA) reports for pharmaceuticals for the German benefit assessment was evaluated by systematically comparing EU JCA and German clinical assessments (CA) based on established assessment elements for HTA and assessing the potential impact of differences on Federal Joint Committee (Gemeinsamer Bundesausschuss, G-BA) ability to derive the therapeutic added value. Methods Identification of all pharmaceuticals undergoing both, EU JCA and German CA between January 2016–June 2020. Qualitative review and data extraction from the assessments, assessment of methodological differences using a hierarchical model. Recommendations for harmonisation were developed and consented with pharmaceutical industry stakeholders. Results Differences with potentially major impact: (1) View on differing treatment algorithms and definition of corresponding subpopulations/respective comparators. (2) Clinical relevance of surrogate/intermediate endpoints. Inclusion of different/surrogate morbidity endpoints resulting in different relative effectiveness conclusions. (3) Tolerance of study interventions not used according to marketing authorisation. (4) Different operationalisation and/or weighting of individual safety endpoints leading to differing relative safety conclusions. Differences with potentially minor impact: (1) Disagreement in risk of bias assessment for overall survival and its robustness against study limitations. (2) Use of patient-reported outcome symptom scales as measurements for health-related quality of life instruments. Conclusion While many synergies between EU JCA and German CA exist, we identified several aspects in HTA methodology that would benefit of harmonisation and ensure the transferability of future EU JCA to the German HTA process without duplicated evaluation requirements. For those, a set of recommendations was developed.

Introduction In the elite space of Haute Couture, fashion is presented through a theatrical array of dynamics—the engagement of specific bodies performing for select audiences in highly curated spaces. Each element is both very precise in its objectives and carefully selected for impact. In this way, the production of Haute Couture makes itself accessible to only a few select members of society. Globally, there are only an estimated 4,000 direct consumers of Haute Couture (Hendrik). Given this limited market, the work of elite couturiers relies on other forms of artistic media, namely film, photography, and increasingly, museum spaces, to reach broader audiences who are then enabled to participate in the fashion ‘space’ via a process of visual consumption. For these audiences, Haute Couture is less about material consumption than it is about the aspirational consumption and contestation of notions of identity. This article uses qualitative textual analysis and draws on semiotic theory to explore symbolism and values in Haute Couture. Semiotics, an approach popularised by the work of Roland Barthes, examines signifiers as elements of the construction of metalanguage and myth. Barthes recognised a broad understanding of language that extended beyond oral and written forms. He acknowledged that a photograph or artefact may also constitute “a kind of speech” (111). Similarly, fashion can be seen as both an important signifier and mode of communication. The model of fashion as communication is one extensively explored within culture studies (e.g. Hall; Lurie). Much of the discussion of semiotics in this literature is predicated on sender/receiver models. These models conceive of fashion as the mechanism through which individual senders communicate to another individual or to collective (and largely passive) audiences (Barnard). Yet, fashion is not a unidirectional form of communication. It can be seen as a dialogical and discursive space of encounter and contestation. To understand the role of Haute Couture as a contested space of identity and socio-political discourse, this article examines the work of Chinese couturier Guo Pei. An artisan such as Guo Pei places the results of needle and thread into spaces of the theatrical, the spectacular, and, significantly, the powerfully socio-political. Guo Pei’s contributions to Haute Couture are extravagant, fantastical productions that also serve as spaces of socio-cultural information exchange and debate. Guo Pei’s creations bring together political history, memory, and fantasy. Here we explore the socio-cultural and political semiotics that emerge when the humble stitch is dramatically amplified onto the Haute Couture runway. We argue that Guo Pei’s work speaks not only to a cultural imaginary but also to the contested nature of gender and socio-political authority in contemporary China. The Politicisation of Fashion in China The majority of literature regarding Chinese fashion in the twentieth and twenty-first centuries has focussed on the use of fashion to communicate socio-political messages (Finnane). This is most clearly seen in analyses of the connections between dress and egalitarian ideals during Mao Zedong’s Cultural Revolution. As Zhang (952-952) notes, revolutionary fashion emphasised simplicity, frugality, and homogenisation. It rejected style choices that reflected both traditional Chinese and Western fashions. In Mao’s China, fashion was utilised by the state and adopted by the populace as a means of reinforcing the regime’s ideological orientations. For example, the ubiquitous Mao suit, worn by both men and women during the Cultural Revolution “was intended not merely as a unisex garment but a means to deemphasise gender altogether” (Feng 79). The Maoist regime’s intention to create a type of social equality through sartorial homogenisation was clear. Reflecting on the ways in which fashion both responded to and shaped women’s positionality, Mao stated, “women are regarded as criminals to begin with, and tall buns and long skirts are the instruments of torture applied to them by men. There is also their facial makeup, which is the brand of the criminal, the jewellery on their hands, which constitutes shackles and their pierced ears and bound feet which represent corporal punishment” (Mao cited in Finnane 23). Mao’s suit—the homogenising militaristic uniform adopted by many citizens—may have been intended as a mechanism for promoting equality, freeing women from the bonds of gendered oppression and all citizens from visual markers of class. Nonetheless, in practice Maoist fashion and policing of appearance during the Cultural Revolution enforced a politics of amnesia and perversely may have “entailed feminizing the undesirable, by conflating woman, bourgeoisie, and colour while also insisting on a type of gender equality that the belted Mao jacket belied” (Chen 161). In work on cultural transformations in the post-Maoist period, Braester argues that since the late 1980s Chinese cultural products—here taken to include artefacts such as Haute Couture—have similarly been defined by the politics of memory and identity. Evocation of historically important symbols and motifs may serve to impose a form of narrative continuity, connecting the present to the past. Yet, as Braester notes, such strategies may belie stability: “to contemplate memory and forgetting is tantamount to acknowledging the temporal and spatial instability of the post-industrial, globalizing world” (435). In this way, cultural products are not only sites of cultural continuity, but also of contestation. Imperial Dreams of Feminine Power The work of Chinese couturier Guo Pei showcases traditional Chinese embroidery techniques alongside more typically Western fashion design practices as a means of demonstrating not only Haute Couturier craftsmanship but also celebrating Chinese imperial culture through nostalgic fantasies in her contemporary designs. Born in Beijing, in 1967, at the beginning of the Chinese Cultural Revolution, Guo Pei studied fashion at the Beijing Second Light Industry School before working in private and state-owned fashion houses. She eventually moved to establish her own fashion design studio and was recognised as “the designer of choice for high society and the political elite” in China (Yoong 19). Her work was catapulted into Western consciousness when her cape, titled ‘Yellow Empress’ was donned by Rihanna for the 2015 Met Gala. The design was a response to an era in which the colour yellow was forbidden to all but the emperor. In the same year, Guo Pei was named an invited member of La Federation de la Haute Couture, becoming the first and only Chinese-born and trained couturier to receive the honour. Recognition of her work at political and socio-economic levels earned her an award for ‘Outstanding Contribution to Economy and Cultural Diplomacy’ by the Asian Couture Federation in 2019. While Maoist fashion influences pursued a vision of gender equality through the ‘unsexing’ of fashion, Guo Pei’s work presents a very different reading of female adornment. One example is her exquisite Snow Queen dress, which draws on imperial motifs in its design. An ensemble of silk, gold embroidery, and Swarovski crystals weighing 50 kilograms, the Snow Queen “characterises Guo Pei’s ideal woman who is noble, resilient and can bear the weight of responsibility” (Yoong 140). In its initial appearance on the Haute Couture runway, the dress was worn by 78-year-old American model, Carmen Dell’Orefice, signalling the equation of age with strength and beauty. Rather than being a site of torture or corporal punishment, as suggested by Mao, the Snow Queen dress positions imagined traditional imperial fashion as a space for celebration and empowerment of the feminine form. The choice of model reinforces this message, while simultaneously contesting global narratives that conflate women’s beauty and physical ability with youthfulness. In this way, fashion can be understood as an intersectional space. On the one hand, Guo Pei's work reinvigorates a particular nostalgic vision of Chinese imperial culture and in doing so pushes back against the socio-political ‘non-fashion’ and uniformity of Maoist dress codes. Yet, on the other hand, positioning her work in the very elite space of Haute Couture serves to reinstate social stratification and class boundaries through the creation of economically inaccessible artefacts: a process that in turn involves the reification and museumification of fashion as material culture. Ideals of femininity, identity, individuality, and the expressions of either creating or dismantling power, are anchored within cultural, social, and temporal landscapes. Benedict Anderson argues that the museumising imagination is “profoundly political” (123). Like sacred texts and maps, fashion as material ephemera evokes and reinforces a sense of continuity and connection to history. Yet, the belonging engendered through engagement with material and imagined pasts is imprecise in its orientation. As much as it is about maintaining threads to an historical past, it is simultaneously an appeal to present possibilities. In his broader analysis, Anderson explores the notion of parallelity, the potentiality not to recreate some geographically or temporally removed place, but to open a space of “living lives parallel …] along the same trajectory” (131). Guo Pei’s creations appeal to a similar museumising imagination. At once, her work evokes both a particular imagined past of imperial grandeur, against instability of the politically shifting present, and appeals to new possibilities of gendered emancipation within that imagined space. Contesting and Complicating East-West Dualism The design process frequently involves borrowing, reinterpretation, and renewal of ideas. The erasure of certain cultural and political aspects of social continuity through the Chinese Cultural Revolution, and the socio-political changes thereafter, have created fertile ground for an artist like Guo Pei. Her palimpsest reaches back through time, picks up those cultural threads of extravagance, and projects them wholesale into the spaces of fashion in the present moment. Cognisance of design intentionality and historical and contemporary fashion discourses influence the various interpretations of fashion semiotics. However, there are also audience-created meanings within the various modes of performance and consumption. Where Kaiser and Green assert that “the process of fashion is inevitably linked to making and sustaining as well as resisting and dismantling power” (1), we can also observe that sartorial semiotics can have different meanings at different times. In the documentary, Yellow Is Forbidden, Guo Pei reflects on shifting semiotics in fashion. Speaking with a client, she remarks that “dragons and phoenixes used to represent the Chinese emperor—now they represent the spirit of the Chinese” (Brettkelly). Once a symbol of sacred, individual power, these iconic signifiers now communicate collective national identity. Both playing with and reimagining not only the grandeur of China’s imperial past, but also the particular role of the feminine form and female power therein, Guo Pei’s corpus evokes and complicates such contestations of power. On the one hand, her work serves to contest homogenising narratives of identity and femininity within China. Equally important, however, are the ways in which this work, which is possible both through and in spite of a Euro-American centric system of patronage within the fashion industry, complicates notions of East-West dualism. For Guo Pei, drawing on broadly accessible visual signifiers of Chinese heritage and culture has been critical in bringing attention to her endeavours. Her work draws significantly from her cultural heritage in terms of colour selections and traditional Chinese embroidery techniques. Symbols and motifs peculiar to Chinese culture are abundant: lotus flowers, dragons, phoenixes, auspicious numbers, and favourable Chinese language characters such as buttons in the shape of ‘double happiness’ (囍) are often present in her designs. Likewise, her techniques pay homage to traditional craft work, including Peranakan beading. The parallelity conjured by these choices is deliberate. In staging Guo Pei’s work for museum exhibitions at museums such as the Asian Civilizations Museum, her designs are often showcased beside the historical artefacts that inspired them (Fu). On her Chinese website, Guo Pei, highlights the historical connections between her designs and traditional Chinese embroidery craft through a sub-section of the “Spirit” header, entitled simply, “Inheritance”. These influences and expressions of Chinese culture are, in Guo Pei's own words her “design language” (Brettkelly). However, Guo Pei has also expressed an ambivalence about her positioning as a Chinese designer. She has maintained that she does not want “to be labelled as a Chinese storyteller ... and thinks about a global audience” (Yoong). In her expression of this desire to both derive power through design choices and historically situated practices and symbols, and simultaneously move beyond nationally bounded identity frameworks, Guo Pei positions herself in a space ‘betwixt and between.’ This is not only a space of encounter between East and West, but also a space that calls into question the limits and possibilities of semiotic expression. Authenticity and Legitimacy Global audiences of fashion rely on social devices of diffusion other than the runway: photography, film, museums, and galleries. Unique to Haute Couture, however, is the way in which such processes are often abstracted, decontextualised and pushed to the extremities of theatrical opulence. De Perthuis argues that to remove context “greatly reduce[s] the social, political, psychological and semiotic meanings” of fashion (151). When iconic motifs are utilised, the western gaze risks falling back on essentialising reification of identity. To this extent, for non-Chinese audiences Guo Pei’s works may serve not so much to problemitise historical and contemporary feminine identities and inheritances, so much as project an essentialisation of Chinese femininity. The double-bind created through Guo Pei’s simultaneous appeal to and resistance of archetypical notions of Chinese identity and femininity complicates the semiotic currency of her work. Moreover, Guo Pei’s work highlights tensions concerning understandings of Chinese culture between those in China and the diaspora. In her process of accessing reference material, Guo Pei has necessarily been driven to travel internationally, due to her concerns about a lack of access to material artefacts within China. She has sought out remnants of her ancestral culture in both the Chinese diaspora as well as material culture designed for export (Yoong; Brettkelly). This borrowing of Chinese design as depicted outside of China proper, alongside the use of western influences and patronage in Guo’s work has resulted in her work being dismissed by critics as “superficial … export ware, reimported” (Thurman). The insinuation that her work is derivative is tinged with denigration. Such critiques question not only the authenticity of the motifs and techniques utilised in Guo Pei’s designs, but also the legitimacy of the narratives of both feminine and Chinese identity communicated therein. Questions of cultural ‘authenticity’ serve to deny how culture, both tangible and intangible, is mutable over time and space. In his work on tourism, Taylor suggests that wherever “the production of authenticity is dependent on some act of (re)production, it is conventionally the past which is seen to hold the model of the original” (9). In this way, legitimacy of semiotic communication in works that evoke a temporally distant past is often seen to be adjudicated through notions of fidelity to the past. This authenticity of the ‘traditional’ associates ‘tradition’ with ‘truth’ and ‘authenticity.’ It is itself a form of mythmaking. As Guo Pei’s work is at once quintessentially Chinese and, through its audiences and capitalist modes of circulation, fundamentally Western, it challenges notions of authenticity and legitimacy both within the fashion world and in broader social discourses. Speaking about similar processes in literary fiction, Colavincenzo notes that works that attempt to “take on the myth of historical discourse and practice … expose the ways in which this discourse is constructed and how it fails to meet the various claims it makes for itself” (143). Rather than reinforcing imagined ‘truths’, appeals to an historical imagination such as that deployed by Guo Pei reveal its contingency. Conclusion In Fashion in Altermodern China, Feng suggests that we can “understand the sartorial as situating a set of visible codes and structures of meaning” (1). More than a reductionistic process of sender/receiver communication, fashion is profoundly embedded with intersectional dialogues. It is not the precision of signifiers, but their instability, fluidity, and mutability that is revealing. Guo Pei’s work offers narratives at the junction of Chinese and foreign, original and derivative, mythical and historical that have an unsettled nature. This ineffable tension between construction and deconstruction draws in both fashion creators and audiences. Whether encountering fashion on the runway, in museum cabinets, or on magazine pages, all renditions rely on its audience to engage with processes of imagination, fantasy, and memory as the first step of comprehending the semiotic languages of cloth. References Anderson, Benedict. Imagined Communities: Reflections on the Origin and Spread of Nationalism. Rev. ed. London: Verso, 2016. Barnard, Malcolm. "Fashion as Communication Revisited." Fashion Theory. Routledge, 2020. 247-258. Barthes, Roland. Mythologies. London: J. Cape, 1972. Braester, Yomi. "The Post-Maoist Politics of Memory." A Companion to Modern Chinese Literature. Ed. Yingjin Zhang. London: John Wiley and Sons. 434-51. Brettkelly, Pietra (dir.). Yellow Is Forbidden. Madman Entertainment, 2019. Chen, Tina Mai. "Dressing for the Party: Clothing, Citizenship, and Gender-Formation in Mao's China." Fashion Theory 5.2 (2001): 143-71. Colavincenzo, Marc. "Trading Fact for Magic—Mythologizing History in Postmodern Historical Fiction." Trading Magic for Fact, Fact for Magic. Ed. Marc Colavincenzo. Brill, 2003. 85-106. De Perthuis, Karen. "The Utopian 'No Place' of the Fashion Photograph." Fashion, Performance and Performativity: The Complex Spaces of Fashion. Eds. Andrea Kollnitz and Marco Pecorari. London: Bloomsbury, 2022. 145-60. Feng, Jie. Fashion in Altermodern China. Dress Cultures. Eds. Reina Lewis and Elizabeth Wilson. London: Bloomsbury Publishing, 2022. Finnane, Antonia. Changing Clothes in China: Fashion, History, Nation. New York: Columbia UP, 2008. Fu, Courtney R. "Guo Pei: Chinese Art and Couture." Fashion Theory 25.1 (2021): 127-140. Hall, Stuart. "Encoding – Decoding." Crime and Media. Ed. Chris Greer. London: Routledge, 2019. Hendrik, Joris. "The History of Haute Couture in Numbers." Vogue (France), 2021. Kaiser, Susan B., and Denise N. Green. Fashion and Cultural Studies. London: Bloomsbury, 2021. Lurie, Alison. The Language of Clothes. London: Bloomsbury, 1992. Taylor, John P. "Authenticity and Sincerity in Tourism." Annals of Tourism Research 28.1 (2001): 7-26. Thurman, Judith. "The Empire's New Clothes – China’s Rich Have Their First Homegrown Haute Couturier." The New Yorker, 2016. Yoong, Jackie. "Guo Pei: Chinese Art and Couture." Singapore: Asian Civilisations Museum, 2019. Zhang, Weiwei. "Politicizing Fashion: Inconspicuous Consumption and Anti-Intellectualism during the Cultural Revolution in China." Journal of Consumer Culture 21.4 (2021): 950-966.

Background: Reperfusion following ischemia can lead to more injuries than ischemia itself especially in diabetic patients. The aim of this study was to evaluate the effect of dexmedetomidine on ischemia-reperfusion injury (IRI) in rats with have hepatic IRI and diabetes mellitus. Methodology: Twenty-eight Wistar Albino rats were randomised into four groups as control (C), diabetic (DC), diabetic with hepatic ischemia-reperfusion injury (DIR), and diabetic but administered dexmedetomidine followed by hepatic IRI (DIRD) groups. Hepatic tissue samples were evaluated histopathologically by semiquantitative methods. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathion s-transpherase (GST), and catalase (CAT) enzyme levels were investigated in liver and kidney tissues as oxidative state parameters. Results: In Group DIR; hepatocyte degeneration, sinusoidal dilatation, pycnotic nucleus, and necrotic cells were found to be more in rat hepatic tissue; while mononuclear cell infiltration was higher in the parenchyme. MDA levels were significantly lower; but SOD levels were significantly higher in Group DIRD with regard to Group DIR. In the IRI induced diabetic rats’ hepatic and nephrotic tissues MDA levels, showing oxidative injury, were found to be lower. SOD levels, showing early antioxidant activity, were higher. Conclusion: The enzymatic findings of our study together with the hepatic histopathology indicate that dexmedetomidine has a potential role to decrease IRI. Key words: Hepatic ischemia reperfusion injury; Diabetes mellitus; Dexmedetomidine; Rat; MDA; SOD Citation: Sezen SC, Işık B, Bilge M, Arslan M, Çomu FM, Öztürk L, Kesimci E, Kavutçu M. Effect of dexmedetomidine on ischemia-reperfusion injury of liver and kidney tissues in experimental diabetes and hepatic ischemia-reperfusion injury induced rats. Anaesth Pain & Intensive Care 2016;20(2):143-149 Received: 21 November 2015; Reviewed: 10, 24 December 2015, 9, 10 June 2016; Corrected: 12 December 2015; Accepted: 10 June 2016 INTRODUCTİON Perioperative acute tissue injury induced by ischemia-reperfusion is a comman clinical event caused by reduced blood supply to the tissue being compromised during major surgery. Ischemia leads to cellular injury by depleting cellular energy deposits and resulting in accumulation of toxic metabolites. The reperfusion of tissues that have remained in ischemic conditions causes even more damage.1 Furthermore hepatic ischemia-reperfusion injury (IRI) demonstrates a strong relationship with peri-operative acute kidney injury.2 The etiology of diabetic complications is strongly associated with increased oxidative stress (OS). Diabetic patients are known to have a high risk of developing OS or IRI which results with tissue failure.3 The most important role in ischemia and reperfusion is played by free oxygen radicals.1 In diabetes, characterized by hyperglycemia, even more free oxygen radicals are produced due to oxidation of glucose and glycosylation of proteins.3 The structures which are most sensitive to free oxygen radicals in the cells are membrane lipids, proteins, nucleic acids and deoxyribonucleic acids.1 It has been reported that endogenous antioxidant enzymes [superoxide dismutase (SOD), glutathion s-transpherase (GST), catalase (CAT)] play an important role to alleviate IRI.4-8 Also some pharmacological agents have certain effects on IRI.1 The anesthetic agents influence endogenous antioxidant systems and free oxygen radical formation.9-12 Dexmedetomidine is a selective α-2 adrenoceptor agonist agent. It has been described as a useful and safe adjunct in many clinical applications. It has been found that it may increase urine output by considerably redistributing cardiac output, inhibiting vasopressin secretion and maintaining renal blood flow and glomerular filtration. Previous studies demonstrated that dexmedetomidine provides protection against renal, focal cerebral, cardiac, testicular, and tourniquet-induced IRI.13-18 Arslan et al observed that dexmedetomidine protected against lipid peroxidation and cellular membrane alterations in hepatic IRI, when given before induction of ischemia.17 Si et al18 demonstrated that dexmedetomidine treatment results in a partial but significant attenuation of renal demage induced by IRI through the inactivation of JAK/STAT signaling pathway in an in vivo model. The efficacy of the dexmedetomidine for IRI in diabetic patient is not resarched yet. The purpose of this experimental study was to evaluate the biochemical and histological effects of dexmedetomidine on hepatic IRI in diabetic rat’s hepatic and renal tissue. METHODOLOGY Animals and Experimental Protocol: This study was conducted in the Physiology Laboratory of Kirikkale University upon the consent of the Experimental Animals Ethics Committee of Kirikkale University. All of the procedures were performed according to the accepted standards of the Guide for the Care and Use of Laboratory Animals. In the study, 28 male Wistar Albino rats, weighing between 250 and 300 g, raised under the same environmental conditions, were used. The rats were kept under 20-21 oC at cycles of 12-hour daylight and 12-hour darkness and had free access to food until 2 hours before the anesthesia procedure. The animals were randomly separated into four groups, each containing 7 rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (Sigma Chemical, St. Louis, MO, USA) at a dose of 65 mg/kg body weight. The blood glucose levels were measured at 72 hrs following this injection. Rats were classified as diabetic if their fasting blood glucose (FBG) levels exceeded 250 mg/dl, and only animals with FBGs of > 250 mg/dl were included in the diabetic groups (dia­betes only, diabetes plus ischemia-reperfusion and diabetes plus dexmedetomidine-ischemia-reperfusion). The rats were kept alive 4 weeks after streptozotocin injection to allow development of chronic dia­betes before they were exposed to ischemia-reperfusion.(19) The rats were weighed before the study. Rats were anesthetized with intraperitoneal ketamine 100 mg/kg. The chest and abdomen were shaved and each animal was fixed in a supine position on the operating table. The abdomen was cleaned with 1% polyvinyl iodine and when dry, the operating field was covered with a sterile drape and median laparotomy was performed. There were four experimental groups (Group C (sham-control; n = 7), (Group DC (diabetes-sham-control; n = 7), Group DIR (diabetes-ischemia-reperfusion; n = 7), and Group DIRD (diabetes-ischemia-reperfusion-dexmedetomidine; n = 7). Sham operation was performed on the rats in Group C and Group DC. The sham operation consisted of mobilization of the hepatic pedicle only. The rats in this group were sacrificed 90 min after the procedure. Hepatic I/R injury was induced in Groups DIR and DIRD respectively with hepatic pedicle clamping using a vascular clamp as in the previous study of Arslan et al.(17) After an ischemic period of 45 min, the vascular clamp was removed. A reperfusion period was maintained for 45 min. In Group DIRD, dexmedetomidine hydrochloride 100 μg/kg, (Precedex 100 μg/2 ml, Abbott®, Abbott Laboratory, North Chicago, Illinois, USA) was administrated via intraperitoneal route 30 minutes before surgery. All the rats were given ketamine 100 mg/kg intraperitoneally and intracardiac blood samples were obtained. Preserving the tissue integrity by avoiding trauma, liver and renal biopsy samples were obtained. Biochemical Analysis: The liver and renal tissues were first washed with cold deionized water to discard blood contamination and then homogenized in a homogenizer. Measurements on cell contest require an initial preparation of the tissues. The preparation procedure may involve grinding of the tissue in a ground glass tissue blender using a rotor driven by a simple electric motor. The homogenizer as a tissue blender similar to the typical kitchen blender is used to emulsify and pulverize the tissue (Heidolph Instruments GMBH & CO KGDiax 900 Germany®) at 1000 U for about 3 min. After centrifugation at 10,000 g for about 60 min, the upper clear layer was taken. MDA levels were determined using the method of Van Ye et al,(20) based on the reaction of MDA with thiobarbituric acid (TBA). In the TBA test reaction, MDA and TBA react in acid pH to form a pink pigment with an absorption maximum at 532 nm. Arbitrary values obtained were compared with a series of standard solutions (1,1,3,3-tetraethoxypropane). Results were expressed as nmol/mg.protein. Part of the homogenate was extracted in ethanol/chloroform mixture (5/3 v/v) to discard the lipid fraction, which caused interferences in the activity measurements of T-SOD, CAT and GST activities. After centrifugation at 10.000 x g for 60 min, the upper clear layer was removed and used for the T-SOD, CAT, GST enzyme activity measurement by methods as described by Durak et al21, Aebi22 and Habig et al23 respectively. One unit of SOD activity was defined as the enzyme protein amount causing 50% inhibition in NBTH2 reduction rate and result were expressed in U/mg protein. The CAT activity method is based on the measurement of absorbance decrease due to H2O2 consumption at 240 nm. The GST activity method is based on the measurement of absorbance changes at 340 nm due to formation of GSH-CDNB complex. Histological determinations: Semiquantitative evaluation technique used by Abdel-Wahhab et al(24) was applied for interpreting the structural changes investigated in hepatic tissues of control and research groups. According to this, (-) (negative point) represents no structural change, while (+) (one positive point) represents mild, (++) (two positive points) medium and (+++) (three positive points) represents severe structural changes. Statistical analysis: The Statistical Package for the Social Sciences (SPSS, Chicago, IL, USA) 20.0 softwre was used for the statistical analysis. Variations in oxidative state parameters, and histopathological examination between study groups were assessed using the Kruskal-Wallis test. The Bonferroni-adjusted Mann-Whitney U-test was used after significant Kruskal-Wallis to determine which groups differed from the others. Results were expressed as mean ± standard deviation (Mean ± SD). Statistical significance was set at a p value < 0.05 for all analyses. RESULTS There was statistically significant difference observed between the groups with respect to findings from the histological changes in the rat liver tissue (hepatocyte degeneration, sinüsoidal dilatation, pycnotic nucleus, prenecrotic cell) determined by light microscopy according to semiquantitative evaluation techniques (p < 0.0001). In Group DIR, hepatocyte degeneration was significantly high compared to Group C, Group DC and Group DIRD (p < 0.0001, p < 0.0001, p = 0.002, respectively), (Table 1, Figure 1-4). Similarly, sinüsoidal dilatation was significantly higher in Group DIR (p < 0.0001, p = 0.004, p = 0.015, respectively). Although, pcynotic nucleus was decreased in Group DIRD, it did not make a significant difference in comparison to Group DIR (p = 0.053), (Table 1, Figure 1-4). The prenecrotic cells were significantly increased in Group DIR, with respect to Group C, Group DC and Group DIRD (p < 0.0001, p = 0.004, p < 0.0001, respectively), (Table 1, Figure 1-4). Table 1. The comparison of histological changes in rat hepatic tissue [Mean ± SD)] p**: Statistical significance was set at a p value < 0.05 for Kruskal-Wallis test *p < 0.05: When compared with Group DIR Figure 1: Light microscopic view of hepatic tissue of Group C (control). VC: vena centralis, *: sinusoids. ®: hepatocytes, k: Kupffer cells, G: glycogen granules, mc: minimal cellular changes, Hematoxilen & Eosin x 40 Figure 2: Light-microscopic view of hepatic tissue of Group DC (diabetes mellitus control) (G: Glycogen granules increased in number, (VC: vena centralis, *:sinusoids. ®:hepatocytes, k:Kupffer cells, G: glycogen granules, mc: minimal cellular changes; Hematoxylin & Eosin x 40) Figure 3: Light-microscopic view of hepatic tissue of Group DIR (Diabetes Mellitus and ischemia-reperfusion) (VC: vena centralis, (H) degenerative and hydrophic hepatocytes, (dej) vena centralis degeneration (centrolobar injury) (*): sinusoid dilatation. (←) pycnotic and hyperchromatic nuclei, MNL: mononuclear cell infiltration, (¯) congestion, K: Kupffer cell hyperplasia, (­) vacuolar degeneration (Hematoxylin & Eosin x 40) Figure 4: Light-microscopic view of hepatic tissue of Group DIRD (Diabetes Mellitus and ischemia-reperfusion together with dexmedetomidine applied group) (VC: vena centralis, (MNL) mononuclear cell infiltration, (dej) hydrophilic degeneration in hepatocytes around vena centralis, (conj) congestion, G: glycogen granules, (←) pycnotic and hyperchromatic nuclei, sinusoid dilatation (*) (Hematoxylin & Eosin x 40) Besides, in liver tissue parenchyma, MN cellular infiltration was a light microscopic finding; and showed significant changes among the groups (p < 0.0001). This was significantly higher in Group DIR, compared to Group C, DC, and DIRD (p < 0.0001, p=0.007, p = 0.007, respectively), (Table 1, Figure 1-4). The enzymatic activity of MDA, SOD and GST in hepatic tissues showed significant differences among the groups [(p = 0.019), (p = 0.034). (p = 0.008) respectively]. MDA enzyme activity was significantly incresed in Group DIR, according to Group C and Group DIRD (p = 0.011, p = 0.016, respectively), (Table 2). In Group DIR SOD enzyme activity was lower with respect to Group C and Group DIRD (p = 0.010, p = 0.038, respectively), (Table 2). The GST enzyme activity was significantly higher in Group DIR, when compared to Group C, DC and DIRD (p = 0.007, p = 0.038, p = 0.039, respectively), (Table 2). Table 2. Oxidative state parameters in rat hepatic tissue [Mean ± SD] p**: Statistical significance was set at a p value < 0.05 for Kruskal-Wallis test *p < 0.05: When compared with Group DIR The enzymatic activity of MDA, SOD in renal tissues, showed significant differences among the groups [(p < 0.0001), (p = 0.008) respectively ]. MDA enzyme activity was significantly incresed in Group DIR, according to Group C and Group DIRD (p < 0.0001, p < 0.0001, respectively). Also MDA enzyme activity level was significantly increased in Group DC, in comparison to Group C and Group DIRD (p = 0.003, p = 0.001, respectively), (Table 3). In Group DIR SOD enzyme activity was lower with respect to Group C and Group DIRD (p = 0.032, p = 0.013, respectively), (Table 3). The GST enzyme activity was significantly higher in Group DIR than the other three groups, however; CAT levels were similar among the groups (Table 3). Table 3: Oxidative state parameters in rat nephrotic tissue [Mean ± SD)] p**: Statistical significance was set at a p value < 0.05 for Kruskal-Wallis test *p < 0.05: When compared with Group DIR DISCUSSION In this study, we have reported the protective effect of dexmedetomidine in experimental hepatic and renal IRI model in the rat by investigating the MDA and SOD levels biochemically. Besides, hepatic histopathological findings also supported our report. Ischemic damage may occur with trauma, hemorrhagic shock, and some surgical interventions, mainly hepatic and renal resections. Reperfusion following ischemia results in even more injury than ischemia itself. IRI is an inflammatory response accompanied by free radical formation, leucocyte migration and activation, sinusoidal endothelial cellular damage, deteoriated microcirculation and coagulation and complement system activation.1 We also detected injury in hepatic and renal tissue caused by reperfusion following ischemia in liver. Experimental and clinical evidence indicates that OS is involved in both the pathogenesis and the complications of diabetes mellitus.25,26 Diabetes mellitus is a serious risk factor for the development of renal and cardiovascular disease. It is also related to fatty changes in the liver.27 Diabetes-related organ damage seems to be the result of multiple mechanisms. Diabetes has been associated with increased free radical reactions and oxidant tissue damage in STZ-induced diabetic rats and also in patients.26Oxidative stress has been implicated in the destruction of pancreatic β-cells28 and could largely contribute to the oxidant tissue damage associated with chronic hyperglycemia.29 A number of reports have shown that antioxidants can attenuate the complications of diabetes in patients30 and in experimental models.28,31 This study demonstrated that diabetes causes a tendency to increase the IRI. There is a lot of investigations related to the pharmacological agents or food supplements applied for decreasing OS and IRI. Antioxidant agents paly an important role in IRI by effecting antioxidant system or lessening the formation of ROS. It has been reported that anesthetic agents too, are effective in oxidative stress.1 During surgical interventions, it seems rational to get benefit from anesthetic agents in prevention of OS caused by IRI instead of using other agents. It has been declared that; dexmedetomidine; as an α-2 agonist with sedative, hypnotic properties; is important in prevention of renal, focal, cerebral, cardiac, testicular and tourniquet-induced IRI.13-18 On the other hand Bostankolu et al. concluded that dexmedetomidine did not have an additional protective role for tournique induced IRI during routine general anesthesia.32 In this study; we have shown that dexmedetomidine has a reducing effect in IRI in diabetic rats. Some biochemical tests and histopathological evaluations are applied for bringing up oxidative stress and IRI in the tissues. Reactive oxygen species (ROS) that appear with reperfusion injury damage cellular structures through the process of the lipid peroxidation of cellular membranes and yield toxic metabolites such as MDA.33 As an important intermidiate product in lipid peroxidation, MDA is used as a sensitive marker of IRI.34 ROS-induced tissue injury is triggered by various defense mechanisms.35 The first defence mechanisms include the antioxidant enzymes of SOD, CAT, and GPx. These endogenous antioxidants are the first lines of defence against oxidative stres and act by scavenging potentially damaging free radical moieties.36 There is a balance between ROS and the scavenging capacity of antioxidant enzymes.1-8 In this study, for evaluation of oxidative damage and antioxidant activity, MDS, SOD, GST and CAT levels were determined in liver and kidney tissues. MDA levels in hepatic and renal tissues were higher in Group DIR compared to Group C and Group DIRD. GST levels were higher in Group DIR compared to all the other three groups. When the groups were arranged from highest to lowest order, with respect to CAT levels, the order was; Group DIR, Group DIRD, Group DC and Group C. However, the difference was not significant. The acute phase reactant MDA, as a marker of OS, was found to be high in Group DIR and low in Group DIRD. This could be interpreted as the presence of protective effect of dexmedetomidine in IRI. IRI developing in splanchnic area causes injury also in the other organs.35 Leithead et al showed that clinically significant hepatic IRI demonstrates a strong relationship with peri-operative acute kidney injury.2 In our experimental research that showed correlation to that of research by Leithead et al. After hepatic IRI in diabetic rats renal OS marker MDA levels were significantly more in Group DIR than Group DIRD. In our study, we observed histopathological changes in the ischemic liver tissue and alterations in the level of MDA, SOD, GST and CAT levels which are OS markers. Histopathological changes of the liver tissues are hepatocyt degeneration, sinusoidal dilatation, nuclear picnosis, celluler necrosis, mononuclear cell infiltrationat paranchimal tissue. These histopathological injury scores were significantly lower in the Group DIRD than those in group DIR. LIMITATION Study limitation is there was no negative control group, as this type of surgical intervention is not possible in rats without anesthesia. CONCLUSION The enzymatic findings of our study together with the hepatic histopathology indicate that dexmedetomidine has a potential role to decrease ischemia-reperfusion injury. Conflict of interest and funding: The authors have not received any funding or benefits from industry or elsewhere to conduct this study. Author contribution: ŞCS: Concept, conduction of the study work and manuscript editing; BI: the main author to write the article; MB & MK: biochemical analysis; MA: manuscript writing; FMÇ: helped us with experimental study; LÖ & EK: collection of data REFERENCES Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology. 2001;94(6):1133. [PubMed] [Free full text] Leithead JA, Armstrong MJ, Corbett C, Andrew M, Kothari C, Gunson BK, et al. Hepatic ischemia reperfusion injury is associated with acute kidney injury following donation after brain death liver transplantation. Transpl Int. 2013;26(11):1116. doi: 10.1111/tri.12175. [PubMed] [Free full text] Panés J, Kurose I, Rodriguez-Vaca D, Anderson DC, Miyasaka M, Tso P, et al. Diabetes exacerbates inflammatory responses to ischemia-reperfusion. Circulation. 1996;93(1):161. [PubMed] [Free full text] Touyz RM. Reactive oxygen species and angiotensin II signaling in vascular cells-implications in cardiovascular disease. Braz J Med Biol Res. 2004;37:1263. [PubMed] [Free full text] Olivares-Corichi IM, Ceballos G, Ortega-Camarillo C, Guzman-Grenfell AM, Hicks JJ. Reactive oxygen species (ROS) induce chemical and structural changes on human insulin in vitro, including alterations in its immunoreactivity. Front Biosci. 2005;10:834. [PubMed] Witko-Sarsat V, Friedlander M, Capeillere-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996;49:1304. [PubMed] Harman D. Free radical theory of aging: An update: Increasing the functional life span. Ann N Y Acad Sci. 2006;1067:10. [PubMed] Nita DA, Nita V, Spulber S, Moldovan M, Popa DP, Zagrean AM, Zagrean L. Oxidative damage following cerebral ischemia depends on reperfusion – a biochemical study in rat. J Cell Mol Med. 2001;5:163–170. [PubMed] [Free full text] Annecke T, Kubitz JC, Kahr S, Hilberath JM, Langer K, Kemming GI, et al. Effects of sevoflurane and propofol on ischaemia-reperfusion injury after thoracic-aortic occlusion in pigs. Br J Anaesth. 2007;98(5):581. [PubMed] [Free full text] De Hert SG, Van der Linden PJ, Cromheecke S, Meeus R, Nelis A, Van Reeth V, ten Broecke PW, et al. Cardioprotective properties of sevoflurane in patients undergoing coronary surgery with cardiopulmonary bypass are related to the modalities of its administration. Anesthesiology. 2004;101(2):299. [PubMed] [Free full text] Yuzer H, Yuzbasioglu MF, Ciralik H, Kurutas EB, Ozkan OV, Bulbuloglu E, et al. Effects of intravenous anesthetics on renal ischemia/reperfusion injury. Ren Fail. 2009;31(4):290. [PubMed] [Free full text] Lee HT, Ota-Setlik A, Fu Y, Nasr SH, Emala CW. Differential protective effects of volatile anesthetics against renal ischemia-reperfusion injury in vivo. Anesthesiology. 2004;101(6):1313. [PubMed] [Free full text] Lai YC, Tsai PS, Huang CJ. Effects of dexmedetomidine on regulating endotoxin-induced up-regulation of inflammatory molecules in murine macrophages. J Surg Res. 2009;154(2):212. doi: 10.1016/j.jss.2008.07.010. [PubMed] Yoshitomi O, Cho S, Hara T, Shibata I, Maekawa T, Ureshino H, Sumikawa K. Direct protective effects of dexmedetomidine against myocardial ischemia-reperfusion injury in anesthetized pigs. Shock. 2012;38(1):92. doi: 10.1097/SHK.0b013e318254d3fb. [PubMed] Jolkkonen J, Puurunen K, Koistinaho J, Kauppinen R, Haapalinna A, Nieminen L, et al. Neuroprotection by the alpha2-adrenoceptor agonist, dexmedetomidine, in rat focal cerebral ischemia. Eur J Pharmacol. 1999;372(1):31. [PubMed] Kocoglu H, Ozturk H, Ozturk H, Yilmaz F, Gulcu N. Effect of dexmedetomidine on ischemia-reperfusion injury in rat kidney: a histopathologic study. Ren Fail. 2009;31(1):70. doi: 10.1080/08860220802546487. [PubMed] Arslan M, Çomu FM, Küçük A, Öztürk L, Yaylak F. Dexmedetomidine protects against lipid peroxidation and erythrocyte deformability alterations in experimental hepatic ischemia reperfusion injury. Libyan J Med. 2012;7. doi: 10.3402/ljm.v7i0.18185 [PubMed] [Free full text] Si Y, Bao H, Han L, Shi H, Zhang Y, Xu L, et al. Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the JAK/STAT signaling activation. J Transl Med. 2013;11(1):141. doi: 10.1186/1479-5876-11-141. [PubMed][Free full text] Türeci E, İş M, Üzüm G, Akyüz F, Ulu MO, Döşoğlu M, et al. Alterations in blood-brain barrier after traumatic brain injury in streptozotocin-induced diabetic rats. J Nervous Sys Surgery 2009;2(2):79. [Free full text] Van Ye TM, Roza AM, Pieper GM, Henderson J Jr, Johnson JP, Adams MB. Inhibition of intestinal lipid peroxidation does not minimize morphological damage. J Surg Res 1993;55:553. [PubMed] Durak I, Canbolat O, Kavutcu M, Öztürk HS, Yurtarslanı Z. Activities of total, cytoplasmic and mihochondrial superoxide dismutase enzymes in sera and pleural fluids from patient with lung cancer. J Clin Lab Anal 1996;10:17. [PubMed] Aebi H. Catalase. In: H.U.Bergmeyer (Ed): Methods of Enzymatic Analysis, Academic Press , New York and London, 1974;pp.673-677. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130. [PubMed] [Free full text] Abdel-Wahhab MA, Nada SA, Arbid MS. Ochratoxicosis: Prevention of developmental toxicity by L-methionine in rats. J Appl Toxicol 1999;19:7. [PubMed] Wolff SP. Diabetes mellitus and free radicals: free radicals, transition metals and oxidative stress in the aetiology of diabetes mellitus and complications. Br Med Bull. 1993;49:642. [PubMed] [Free full text] West IC. Radicals and oxidative stress in diabetes. Diabet Med. 2000;17:171–180. [PubMed] Wanless IR, Lentz JS. Fatty liver hepatitis (steatohepatitis) and obesity: an autopsy study with analysis risk factors. Hepatology. 1990;12:1106. [PubMed] Hotta M, Tashiro F, Ikegami H, Niwa H, Ogihara T, Yodoi J, Miyazaki J. Pancreatic cell-specific expression of thioredoxin, an antioxidative and antiapoptotic protein, prevents autoimmune and streptozotocin-induced diabetes. J Exp Med. 1998;188:1445. [PubMed] [Free full text] Baynes JW. Role of oxidative stress in the development of complications in diabetes. Diabetes. 1991;40:405. [PubMed] Borcea V, Nourooz-Zadeh J, Wolff SP, Klevesath M, Hofmann M, Urich H, et al. α-Lipoic acid decreases oxidative stress even in diabetic patients with poor glycemic control and albuminuria. Free Radic Biol Med. 1999;26:1495. [PubMed] Fitzl G, Martin R, Dettmer D, Hermsdorf V, Drews H, Welt K. Protective effect of ginkgo biloba extract EGb 761 on myocardium of experimentally diabetic rats, I: ultrastructural and biochemical investigation on cardiomyocytes. Exp Toxicol Pathol. 1999;51:189. [PubMed] Bostankolu E, Ayoglu H, Yurtlu S, Okyay RD, Erdogan G, Deniz Y, et al. Dexmedetomidine did not reduce the effects of tourniquet-induced ischemia-reperfusion injury during general anesthesia. Kaohsiung J Med Sci. 2013;29(2):75. doi: 10.1016/j.kjms.2012.08.013. [PubMed] [Free full text] Wakai A, Wang JH, Winter DC, Street JT, O’Sullivan RG, Redmond HP. Tourniquet-induced systemic inflammatory response in extremity surgery. J Trauma 2001;51:922. [PubMed] Concannon MJ, Kester CG, Welsh CF, Puckett CL. Patterns of free-radical production after tourniquet ischemia implications for the hand surgeon. Plast Reconstr Surg 1992;89:846. [PubMed] Grisham MB, Granger DN. Free radicals: reactive metabolites of oxygen as mediators of postischemic reperfusion injury. In: Martson A, Bulkley GB, Fiddian-Green RG, Haglund U, editors. Splanchnic İschemia and Multiple Organ Failure. St Louis, MO: Mosby;1989. pp. 135–144. Mccard JM. The evolution of free radicals and oxidative stress. Am J Med 2000;108:652. [PubMed]