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Journal articles on the topic 'Western blot'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Dock, N. L., S. H. Kleinman, M. A. Rayfield, C. A. Schable, A. E. Williams, and R. Y. Dodd. "Western Blot Advisory." Infection Control & Hospital Epidemiology 11, no. 8 (1990): 433. http://dx.doi.org/10.1086/646203.

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2

Pan, Wenying, Wei Chen, and Xingyu Jiang. "Microfluidic Western Blot." Analytical Chemistry 82, no. 10 (2010): 3974–76. http://dx.doi.org/10.1021/ac1000493.

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3

Rieves, Rafel D. "The Western Blot." Annals of Internal Medicine 125, no. 4 (1996): 343. http://dx.doi.org/10.7326/0003-4819-125-4-199608150-00016.

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4

Butler, Trent A. J., Jonathan W. Paul, Eng-Cheng Chan, Roger Smith, and Jorge M. Tolosa. "Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures." BioMed Research International 2019 (January 21, 2019): 1–15. http://dx.doi.org/10.1155/2019/5214821.

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Densitometry data generated for Western blots are commonly used to compare protein abundance between samples. In the last decade, it has become apparent that assumptions underpinning these comparisons are often violated in studies reporting Western blot data in the literature. These violations can lead to erroneous interpretations of data and may contribute to poor reproducibility of research. We assessed the reliability of Western blot data obtained to study human myometrial tissue proteins. We ran dilution series of protein lysates to explore the linearity of densitometry data. Proteins anal

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5

Kroon, Cristina, Larissa Breuer, Lydia Jones, et al. "Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them." PLOS Biology 20, no. 9 (2022): e3001783. http://dx.doi.org/10.1371/journal.pbio.3001783.

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Western blotting is a standard laboratory method used to detect proteins and assess their expression levels. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader’s ability to evaluate or reproduce western blot results. While several groups have studied the prevalence of image manipulation or provided recommendations for improving western blotting, data on the prevalence of common publication practices are scarce. We systematically examined 551 articles published in the top 25% of journals in neurosciences (n = 151) and cell biolog

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6

Nybo, Kristie. "Western blot: protein migration." BioTechniques 53, no. 1 (2012): 23–24. http://dx.doi.org/10.2144/000113887.

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7

Dock, N. L., S. H. Kleinman, M. A. Rayfield, C. A. Schable, A. E. Williams, and R. Y. Dodd. "Appendix: Western Blot Advisory." Infection Control and Hospital Epidemiology 11, no. 8 (1990): 433. http://dx.doi.org/10.2307/30146854.

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8

Turner, Valendar F. "HIV western blot test." Medical Journal of Australia 160, no. 12 (1994): 807–8. http://dx.doi.org/10.5694/j.1326-5377.1994.tb125971.x.

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9

Dax, Elizabeth. "HIV western blot test." Medical Journal of Australia 160, no. 12 (1994): 808. http://dx.doi.org/10.5694/j.1326-5377.1994.tb125972.x.

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10

Dutton, Gail. "Reinventing the Western Blot." Genetic Engineering & Biotechnology News 33, no. 16 (2013): 10–11. http://dx.doi.org/10.1089/gen.33.16.02.

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11

Kaufmann, Scott H., Charles M. Ewing, and Joel H. Shaper. "The erasable Western blot." Analytical Biochemistry 161, no. 1 (1987): 89–95. http://dx.doi.org/10.1016/0003-2697(87)90656-7.

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12

Healey, David S., William J. Maskill, Teresa S. Howard, et al. "HIV-1 Western blot." Aids 6, no. 7 (1992): 629–34. http://dx.doi.org/10.1097/00002030-199207000-00003.

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13

Mavin, S., R. M. Milner, R. Evans, J. M. W. Chatterton, A. W. L. Joss, and D. O. Ho-Yen. "The use of local isolates in Western blots improves serological diagnosis of Lyme disease in Scotland." Journal of Medical Microbiology 56, no. 1 (2007): 47–51. http://dx.doi.org/10.1099/jmm.0.46793-0.

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Nine Scottish Borrelia burgdorferi isolates were investigated in IgG Western blot tests. Sera previously found to be positive and negative when tested by routine Western blots prepared from reference strain B. burgdorferi sensu stricto antigen had different outcomes with these isolates. Two isolates, E5 (Borrelia afzelii) and G4 (B. burgdorferi sensu stricto) performed well, reproducing Western blot-positive results in 90 and 95 % of tests, respectively. When antigens from both isolates were incorporated into a single IgG Western blot, the results of a panel of sera were improved when compared

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14

Gibbons, Janay. "Western blot: Protein transfer overview." North American Journal of Medical Sciences 6, no. 3 (2014): 158. http://dx.doi.org/10.4103/1947-2714.128481.

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15

Jiang, Hezi. "Experimental Study on Western Blot." IOP Conference Series: Earth and Environmental Science 615 (December 12, 2020): 012110. http://dx.doi.org/10.1088/1755-1315/615/1/012110.

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16

Laperche, S., M. H. Elghouzzi, C. Rannou, and V. Faucher. "Western-blot ou immunoblot VIH?" Immuno-analyse & Biologie Spécialisée 12, no. 4 (1997): 173–80. http://dx.doi.org/10.1016/s0923-2532(97)89653-6.

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17

Lee, Nancy, Sun-Qu Zhang, and Douglas Testa. "A rapid multicolor Western blot." Journal of Immunological Methods 106, no. 1 (1988): 27–30. http://dx.doi.org/10.1016/0022-1759(88)90267-0.

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18

Fawcett, Paul T., Carlos D. Rosé, Kathleen M. Gibney, and Robert A. Doughty. "Comparison of Immunodot and Western Blot Assays for Diagnosing Lyme Borreliosis." Clinical Diagnostic Laboratory Immunology 5, no. 4 (1998): 503–6. http://dx.doi.org/10.1128/cdli.5.4.503-506.1998.

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ABSTRACT Two commercially available serologic tests for use in diagnosing Lyme borreliosis were evaluated by using a test panel comprised of sera from patients diagnosed with Lyme borreliosis, non-Lyme disease controls, and healthy subjects. The test methods examined were a Western blot assay and an immunodot assay. The study was initiated to determine how the immunodot assay, which contains purified and recombinant proteins to those borrelial antigens recommended for immunoglobulin M (IgM) detection in the Dearborn criteria, would compare with the Western blot assay as a confirmatory method f

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19

Patel, P. C., L. Aubin, and J. Côte. "Use of monoclonal and polyclonal antibodies to detect prostatic acid phosphatase by immunoblotting." Clinical Chemistry 32, no. 10 (1986): 1832–35. http://dx.doi.org/10.1093/clinchem/32.10.1832.

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Abstract We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use of polyclonal antisera, but not with monoclonal antibodies. On the other hand, using a dot blot assay, we could easily detect PAP with both polyclonal and monoclonal antibodies.

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20

Taylor, Sean C., and Anton Posch. "The Design of a Quantitative Western Blot Experiment." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/361590.

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Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a protein target in a complex sample. Although there have been significant advances in both the imaging and reagent technologies to improve sensitivity, dynamic range of detection, and the applicability of multiplexed target detection, the basic technique has remained essentially unchanged. In the past, western blotting was used simply to detect a specific target protein in a complex mixture, but now journal editors and reviewers are requesting the quantitative interpretatio

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21

Ma’arif, Burhan, Fariza Amanatul Sholihah, Anisah Mahardiani, Begum Fauziyah, Denis Mery Mirza, and Mangestuti Agil. "Runt-Related Transcription Factor 2 (Runx2) Measurement in Phytoestrogen-Induced Bone: A Comparison of Western Blot and Immunohistochemistry Methods." Biomedical and Pharmacology Journal 15, no. 2 (2022): 1039–52. http://dx.doi.org/10.13005/bpj/2439.

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Estrogen deficiency can contribute to osteoporosis in postmenopausal women. Phytoestrogens are becoming more widely recognized as potential estrogen replacement therapy. The administration of phytoestrogens can cause bone formation, which is marked by an increase in Runx2 expression in osteoblast cells and can be seen using western blot and immunohistochemistry approaches. This review aimed to compare the detection methods of Runx2 in phytoestrogen-induced bone tissue using western blots and immunohistochemistry. Selectivity, sensitivity, processing time, and cost-effectiveness were the parame

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22

Lakshmi, V., VD Teja, and T. Sudha. "Western blot profile in HIV infection." Indian Journal of Dermatology, Venereology and Leprology 72, no. 5 (2006): 357. http://dx.doi.org/10.4103/0378-6323.27752.

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23

Mann, Matthias. "Can Proteomics Retire the Western Blot?" Journal of Proteome Research 7, no. 8 (2008): 3065. http://dx.doi.org/10.1021/pr800463v.

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24

Oceguera, Leopoldo F., Robert E. Chiles, Peter J. Patiris, Carl V. Hanson, Leslie H. Tobler, and Michael P. Busch. "Flavivirus Serology by Western Blot Analysis." American Journal of Tropical Medicine and Hygiene 77, no. 1 (2007): 159–63. http://dx.doi.org/10.4269/ajtmh.2007.77.159.

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25

Bravo-Martinez, Jorge. "Open source automated western blot processor." HardwareX 6 (October 2019): e00088. http://dx.doi.org/10.1016/j.ohx.2019.e00088.

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26

Choumert, Ariane, Carla Fernandez, Marie-Line Jacquemont, France Leturcq, and J. Andoni Urtizberea. "Le Western-Blot à la rescousse…" Les Cahiers de Myologie, no. 15 (June 2017): 6–9. http://dx.doi.org/10.1051/myolog/201715002.

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27

Kiyokawa, Tetsuyuki, Kazunari Yamaguchi, Yoko Nishimura, Naomi Fukuoka, Toshiki Watanabe, and Kiyoshi Takatsuki. "Western blot criteria for HTLV-I." Lancet 338, no. 8762 (1991): 312. http://dx.doi.org/10.1016/0140-6736(91)90450-4.

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28

Mortimer. "The fallibility of HIV western blot." Lancet 337, no. 8736 (1991): 286–87. http://dx.doi.org/10.1016/0140-6736(91)90884-r.

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29

Miller-Fleming, Leonor, Heesun Cheong, Pedro Antas, and Daniel J. Klionsky. "Detection ofSaccharomyces cerevisiaeAtg13 by western blot." Autophagy 10, no. 3 (2014): 514–17. http://dx.doi.org/10.4161/auto.27707.

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30

Jin, Shi, and Robert T. Kennedy. "New developments in Western blot technology." Chinese Chemical Letters 26, no. 4 (2015): 416–18. http://dx.doi.org/10.1016/j.cclet.2015.01.021.

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31

Schrödel, Andrea. "Western-Blot Teil 2: Die Immunreaktion." Biologie in unserer Zeit 40, no. 3 (2010): 153. http://dx.doi.org/10.1002/biuz.201090043.

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32

Gerrard, JM, D. Lint, PJ Sims, et al. "Identification of a platelet dense granule membrane protein that is deficient in a patient with the Hermansky-Pudlak syndrome." Blood 77, no. 1 (1991): 101–12. http://dx.doi.org/10.1182/blood.v77.1.101.101.

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Abstract Monoclonal antibodies were raised after injecting mice with isolated human dense granules. Several of these monoclonals were found to recognize a 40-Kd dense granule membrane protein. Western blot and immunofluorescent analysis confirmed the dense-granule specificity. After thrombin activation, the protein was found in patches on the external platelet membrane. By Western blot and slot blot analysis, the protein was found to be markedly deficient in a patient with the Hermansky-Pudlak syndrome. Studies of neutrophils and endothelial cells show the presence of immunologically related g

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33

Gerrard, JM, D. Lint, PJ Sims, et al. "Identification of a platelet dense granule membrane protein that is deficient in a patient with the Hermansky-Pudlak syndrome." Blood 77, no. 1 (1991): 101–12. http://dx.doi.org/10.1182/blood.v77.1.101.bloodjournal771101.

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Monoclonal antibodies were raised after injecting mice with isolated human dense granules. Several of these monoclonals were found to recognize a 40-Kd dense granule membrane protein. Western blot and immunofluorescent analysis confirmed the dense-granule specificity. After thrombin activation, the protein was found in patches on the external platelet membrane. By Western blot and slot blot analysis, the protein was found to be markedly deficient in a patient with the Hermansky-Pudlak syndrome. Studies of neutrophils and endothelial cells show the presence of immunologically related granule-me

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34

Lamees Jamal Talib, Basma Talib Al-Sudani, and Mustafa Ghazi Al-Abbassi. "Aptamer Validation by Western Blot–an overview." Al Mustansiriyah Journal of Pharmaceutical Sciences 20, no. 4 (2022): 122–31. http://dx.doi.org/10.32947/ajps.v20i4.782.

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Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract. The whole process of western blot consists of three stages and can be described briefly as separation of  protein. followed by transportation to a solid membrane and finally detection of the target by an antibody. Western blot technique is usually used for the detection of proteins but also can be used to detect other molecules such as aptamers. Aptamers can be defined as a short-stra

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35

Raoult, D., and G. A. Dasch. "Line blot and western blot immunoassays for diagnosis of Mediterranean spotted fever." Journal of Clinical Microbiology 27, no. 9 (1989): 2073–79. http://dx.doi.org/10.1128/jcm.27.9.2073-2079.1989.

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36

Yom, Heng-Cherl, та Robert D. Bremel. "Xerographic paper as a transfer medium for Western blots: Quantification of bovine αS1-casein by Western blot". Analytical Biochemistry 200, № 2 (1992): 249–53. http://dx.doi.org/10.1016/0003-2697(92)90461-f.

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37

Gazi, Md Jamal Uddin, Md Ruhul Amin Miah, Sharmeen Ahmed, and Abu Naser Ibne Sattar. "Comparison of Western Blot Technique with Microscopy and Culture for Diagnosis of Tuberculosis." Bangladesh Journal of Medical Microbiology 1, no. 1 (2016): 21–24. http://dx.doi.org/10.3329/bjmm.v1i1.20491.

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Diagnosis of tuberculosis is usually done by microscopy for AFB and other tests. But each test has different limitation. This study was carried out to compare western blot technique with microscopy and culture for diagnosis of tuberculosis. Sputum and other samples were collected from 112 TB patients for bacteriological diagnosis by microscopy and culture for AFB. Serum samples were collected from 112 TB patients and 50 control subjects for detection of antibody response by western blot. For western blot analysis, Mycobacterium tuberculosis sonicate antigen extract was fractionated by electrop

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38

Navarre, Jennifer, Alvin J. Bradford, Benjamin C. Calhoun, and James R. Goldenring. "Quenching of Endogenous Peroxidase in Western Blot." BioTechniques 21, no. 6 (1996): 990–92. http://dx.doi.org/10.2144/96216bm04.

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39

Yang, Ping-Chang, and Tahrin Mahmood. "Western blot: Technique, theory, and trouble shooting." North American Journal of Medical Sciences 4, no. 9 (2012): 429. http://dx.doi.org/10.4103/1947-2714.100998.

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40

Yang, Ping-Chang, Zhi-Qiang Liu, and Tahrin Mahmood. "Western blot: Technique, theory and trouble shooting." North American Journal of Medical Sciences 6, no. 3 (2014): 160. http://dx.doi.org/10.4103/1947-2714.128482.

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41

Yamaguchi, Keiko. "Western blot analysis of HeLa cell ferritin." SEIBUTSU BUTSURI KAGAKU 35, no. 6 (1991): 441–44. http://dx.doi.org/10.2198/sbk.35.441.

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42

Liu, Bing, Hanyu Tang, Qian Liu, et al. "Core-shell SERS nanotags-based western blot." Talanta 253 (February 2023): 123888. http://dx.doi.org/10.1016/j.talanta.2022.123888.

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43

Emmanuel, J. C., H. J. Smith, and L. E. Paterson. "Western Blot Testing among Zimbabwean HIV Patients." Vox Sanguinis 55, no. 4 (1988): 247–48. http://dx.doi.org/10.1159/000461885.

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44

&NA;. "Shortcomings of Western blot for HIV confirmation." Inpharma Weekly &NA;, no. 774 (1991): 4. http://dx.doi.org/10.2165/00128413-199107740-00008.

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45

Lal, Ashish, Susan R. Haynes, and Myriam Gorospe. "Clean western blot signals from immunoprecipitated samples." Molecular and Cellular Probes 19, no. 6 (2005): 385–88. http://dx.doi.org/10.1016/j.mcp.2005.06.007.

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46

MacPhee, Daniel J. "Methodological considerations for improving Western blot analysis." Journal of Pharmacological and Toxicological Methods 61, no. 2 (2010): 171–77. http://dx.doi.org/10.1016/j.vascn.2009.12.001.

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47

Chowrimootoo, G. "Western blot analysis in patients with hypocaeruloplasminaemia." QJM 90, no. 3 (1997): 197–202. http://dx.doi.org/10.1093/qjmed/90.3.197.

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48

Cummings, P. J. "Simulated western blot for the science curriculum." Biochemical Education 25, no. 1 (1997): 39–40. http://dx.doi.org/10.1016/s0307-4412(96)00162-8.

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49

Grenouillet, Frédéric, Nadège Cabrolier, Florence Grenouillet, and Sandrine Roussel. "Sérologie aspergillaire : corrélation immunoélectrophorèse - Western Blot Aspergillus." Journal de Mycologie Médicale 25, no. 2 (2015): e109. http://dx.doi.org/10.1016/j.mycmed.2015.02.032.

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50

King, Rachel, Philippe Van De Perre, Susan Allen, Antoine Serufilira, and Jacques Homsy. "Predictive value of indeterminate HIV western blot." Lancet 338, no. 8777 (1991): 1278. http://dx.doi.org/10.1016/0140-6736(91)92149-v.

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