Journal articles on the topic 'Wet 38 van 2005'

Die Gautengse afdeling van die hooggeregshof (Pretoria) het in Wilsnach NO v M beslis dat die natuurlike vader van ’n kind wat intestaat gesterf het nie as die “ouer” van die kind gekwalifiseer het vir doeleindes van die Wet op Intestate Erfopvolging 81 van 1987 nie. Die kind se ouma aan moederskant het volgens die hof egter wel gekwalifiseer om as “ouer” en daarom as die kind se intestate erfgenaam erken te word. Die hof het tot hierdie slotsom gekom nadat ’n kinderhof die ouerlike verantwoordelikhede en regte van die natuurlike vader beëindig het en ouerlike verantwoordelikhede en regte aan die kind se ouma toegeken het. Die hof was van mening dat die betekenis van die woord “ouer” in die Wet op Intestate Erfopvolging nie slegs op ’n biologiese band dui nie en dat die natuurlike vader nie voldoen het aan die definisie van wat as ’n ouer beskou behoort te word nie. Faktore wat in hierdie verband ’n rol gespeel het, was dat die vader onder andere nooit vir die kind gesorg, hom onderhou, as sy voog opgetree, hom teen mishandeling beskerm of hom begelei het om sy volle potensiaal te bereik nie. Die erkenning van die vader as ouer en dus as intestate erfgenaam sou volgens die hof teen die beste belange van die kind ingedruis het. Die ouma, wat wél bogenoemde ouerlike funksies vervul het, kon egter wel as ouer erken word en sy kon dus saam met die kind se natuurlike moeder intestaat van haar kleinseun erf. In hierdie bydrae word aan die hand gedoen dat, alhoewel hierdie beslissing moontlik sommige se sin vir billikheid mag bevredig, die hof nie korrek was in die wyse waarop hierdie resultaat bereik is nie. Daar word op enkele fundamentele verskille gewys tussen die hof se hantering van die probleem in die Wilsnach-saak en dié van die howe in hul vroeëre herinterpretasie van die begrip “eggenoot” in die Wet op Intestate Erfopvolging. Ook is die hof se toepassing van die beginsel van die beste belange van die kind en sy invoering van die Kinderwet 38 van 2005 se definisie van “ouer” in hierdie konteks omstrede. Wat die bereiking van ’n billike resultaat betref, word op enkele alternatiewe gewys wat die hof sou kon oorweeg het. Regshervorming rondom hierdie kwessie – in soverre dit wel nodig is – behoort eerder op ’n deurdagte en gestruktureerde wyse te geskied.

A needs assessment done among HIV-positive (HIV+ve) people in Botswana in 2000 indicated that these people required social support. Based on these results, a buddy system for and by HIV+ve women was instituted in Botswana during 2002. This study examined the impact of the buddy system on the self-care behaviours of 116 HIV+ve women volunteers who used the services of COCEPWA (Coping Centre for People with Aids) during 2002. The convenience sample comprised 39 buddies who completed the buddy training programme, 39 patients assigned to the 39 buddies and 38 controls who lived in areas where the buddy programme did not operate. The results indicate that HIV+ve patients who had buddies showed improved self-care behaviours from April 2002 until November 2002 compared to the controls. These self-care behaviours encompassed informing a number of other people about their HIV+ve status, compliance with tuberculosis treatment, CD4 quantification and adherence to antiretroviral therapy. Although the differences were not always statistically significant, the patients showed greater improvements than the controls in all self-care behaviours. Thus the buddy system might have assisted and empowered the patients to achieve higher levels of self-care behaviours than the controls. Opsomming ’n Behoeftebepaling wat in 2000 van MIV-positiewe (MIV+we) persone in Botswana gedoen is, het getoon dat hierdie mense sosiale ondersteuning nodig gehad het. Gebaseer op hierdie bevindinge is ’n “buddy”-stelsel vir en deur MIV+we vroue gedurende 2002 in die land ingestel. Hierdie studie het die impak van die “buddy”-stelsel op die selfsorggedrag van 116 MIV+we vroulike vrywilligers ondersoek wat die dienste van COCEPWA (Coping Centre for People with AIDS) gedurende 2002 benut het. Die gerieflikheidsteekproef het uit 39 “buddies” bestaan wat die “buddy”-opleidingsprogram voltooi het, 39 pasiënte wat aan die 39 “buddies” toegewys is en 38 kontrolepatiënte wat in areas gewoon het waar die “buddy”-program nog nie in werking gestel is nie. Die bevindinge dui aan dat MIV+we pasiënte wat “buddies” gehad het verbeterde selfsorggedrag van April 2002 tot November 2002 getoon het, in vergelyking met die kontrolegroep. Selfsorggedrag is aangedui deur die aantal ander persone wat ingelig is omtrent die individu se MIV+we status, die nakoming van tuberkulosebehandeling, CD4-bepalings en die nakoming van antiretrovirale behandeling. Alhoewel die verskille nie altyd statisties beduidend was nie, het die pasiënte groter verbeteringe getoon in vergelyking met die kontrolegroep in alle aspekte van selfsorggedrag. Dus is dit moontlik dat die “buddy”-stelsel die pasiënte ondersteun en bemagtig het, wat hulle in staat gestel het om ’n groter mate van selfsorggedrag te bereik as die kontrolegroep.

In Africa, the natural history of SCD is often assumed to be same to the African Diaspora in the US, Jamaica, Europe or Latin America. Yet the environment can be different, including different pathogen exposure, such as malaria. To help better understand this, over 2000 references were identified using the names of all current or past names of African continent countries and the truncated word sickl$, followed by secondary nested and cross reference searches. Six cases series describing causes of death were identified, representing 182 children (Ndugwa, 1973, Athale, 1994, Koko, 1998, Diagne, 2000, Rahimy, 2003, Van-Dunem, 2007). Gender was reported in 172, 73 were female (42%). Age was reported in 118, 52 were < 5 years (44%). Four studies described some impediment to care or arrival for care in extremis in1/4 to over ½ of patients that died. In Uganda, 9/12 (75%) patient died at home. In Gabon 6/23 (26%) patients died within 4 hours of reaching the hospital and 11/23 (48%) within 24 hours. In Benin 2/10 (20%) died of splenic sequestration diagnosed at home; 38/64 (53%) of patients in Mozambique that died, lived outside of the capital. Causes of death were identified in 146 individuals. These included: fever/sepsis: n=59 (40%), including meningitis: n=15 (10%) and pyelonephritis: n=2 (1%); acute anemia: n=43 (29%), including spleen sequestration: n=28 (19%) and aplastic anemia: n=8 (5%); pain: n=22 (15%); acute chest syndrome/pneumonia: n=18 (12%); CNS: n=8 (5%), including stroke: n=4 (3%), seizure/ coma: n=5 (3%); liver disease: n=5 (3%) including hepatitis: n=3 (2%); Other: n=19 (13%) including wasting/ malnutrition: n=7 (5%), heart failure/cardiomyopathy: n=4 (3%), diarrhea and vomiting: n=3 (2%), transfusion reaction: n=2 (1%). Infectious pathogens were identified in 26, including malaria: n=10 (38%), S. pneumoniae: n=3 (12%), Salmonella: n=2 (8%), H. influenza, Klebsiella and Citrobacter: n=1 (4%) each; viral agents were reported in n=8 (31%) including HBV: n=5 (19%), HIV: n=3 (12%). Reported general population hemoglobinopathy surveys after birth revealed the following Relative Risk (RR) of observing individuals with hemoglobin SS compared to Hardy Weinberg expected frequencies (some age cohorts overlap; Tanzania '56, Benin '09, Burkina Faso '70, Central African Republic'75, Gabon'65/'80, Gambia'56, Ghana '56/‘57/'00/'10, Kenya '04/'10, Malawi '72/'00/'04, Mozambique '86, Nigeria '56/'70/'79/'81/'84/'05, Senegal '69, Sierra Leone '56). Age 0-1 years, total n=2112 observed n=22 (1.0%), expected n=16.5 (0.8%), RR=1.3 (95% CI=0.7,2.5), p=0.441. Age 0-6 years, total n=4078; observed n=39 (1.0%); expected n=40.6 (1.0%); RR=1.0 (95% CI=0.6,1.5), p=0.925. Age 5-19 years, total n=1880; observed n= 5 (0.3%); expected n= 24.8 (1.3%); RR=0.2 (95% CI=0.1,0.5); p<0.001. Adults, total n=12814; observed n= 20 (0.2%); expected n= 118.9 (0.9%); RR=0.2 (95% CI=0.1,0.3), p<0.001. Pregnant, total n=5815; observed n= 19 (0.3%); expected n= 78.5 (1.3%), RR=0.2 (95% CI=0.1,0.4), p<0.001. Cohorts of children with SCD are indicated in the table. In summary, access to care, as well as acute anemias are a frequent cause of mortality. Along with viral pathogens and transfusion related deaths this indicates the importance of a safe blood supply. By adulthood, the observed frequency of individuals with SCD is only 1/5 of expected. However, reported clinic cohorts suggest similar if not better survival than in the general population, possibly due to lost to follow up, but also malaria/bacterial infection prevention and nutritional support. Careful prospective studies are needed.TableCohorts of children in Africa with Sickle Cell AnemiaCountryAge median years, (range)Death/TotalnFollow up yearsPatient-yearsDeaths/100 patient-yearsU5M/100 child-yrs♦Uganda, 735 -9, (0-20)12/6282--2.7Senegal, ‘008 (0-22)11/323710331.12.2Senegal, 03330/55612--2.2Benin,032.910/2361.5-6.59831.02.4Kenya,096 (0-13)2/1241.21181.72.7♦: Under five year old mortality 2009 (source: Unicef), divided by 5Prophylactic interventions: Uganda: chloroquine; Senegal: chloroquine (wet season), nets, penicillin prophylaxis <5 yrs, folic acid, parasite treatment & iron supplement as needed; Benin: chloroquine, nets, penicillin prophylaxis, antibiotics for fever, folic acid, nutritional support; Kenya; Proguanil, folic acid, nutritional support, parasite treatment & iron supplement as needed. Disclosures: No relevant conflicts of interest to declare.

Dit jaar is het tien jaar geleden dat de International Financial Reporting Standards (IFRSs) in de Europese Unie kracht van wet kregen. Op basis van een uit 2002 daterende Europese verordening – de zogenoemde IASverordening – moeten beursgenoteerde ondernemingen in de Europese Unie met ingang van boekjaar 2005 hun geconsolideerde jaarrekening verplicht opstellen in overeenstemming met de IFRSs. Het doel van de introductie van de IFRSs was het creeren van een gelijk speelveld in de Europese Unie op het gebied van de externe nanciële verslaggeving om zodoende oneerlijke concurrentie tussen de lidstaten te voorkomen en de efciënte van de Europese kapitaalmarkt te bevorderen.

‘Het lijkt mij dus niet vol te houden dat de komst van IFRS betekent dat regels het gaan win - nen van het inzicht. Integen deel zelfs.’ (J.H.M. Willems, voorzitter Ondernemingskamer van het Gerechtshof te Amsterdam) Vanaf de jaarrekeningen 2006 gaat het dan eindelijk gebeuren: het lang verwachte toezicht op de jaarrekeningen van ondernemingen, uit te voeren door de Autoriteit Financiële Markten (AFM). Eind oktober 2005 heeft het Ministerie van Financiën het voorstel voor de Wet toezicht financiële verslaggeving (WTFV) openbaar gemaakt. De Eerste Kamer heeft inmiddels het voorstel goedgekeurd. Hiermee wordt in Nederland aangesloten bij een al in vele landen bestaande cultuur van toezicht. Het is mede een uitvloeisel van de toepassing van IFRS in Europa, waarbij ‘endorsement’ (goedkeuring door de EU) en ‘enforcement’ (toezicht) belangrijke ingrediënten zijn. Coördinatie van het toezicht in Europa vindt plaats door het Committee of European Securities Regulators (CESR), naar verwachting een voorloper van één centrale Europese toezichthouder (Delger en Koster, 2006).

Background: Ventricular assist device (VAD) patients are at high risk for morbidities and mortality. One potentially beneficial component of the Joint Commission VAD Certification process is the requirement that individual VAD programs select 4 performance measures to improve and optimize patients’ clinical outcomes. Problem Statement: Review of patient data after our program’s first certification visit in 2008 showed that, compared to national recommendations and published reports, our patients had suboptimal outcomes in 4 areas after device implantation: length of hospital stay, receipt of early (<48 hours) postsurgical physical therapy, driveline infection incidence, and adequacy of nutritional status (prealbumin ≥18 mg/dL). Methods: Plan-Do-Study-Act processes were implemented to shorten length of stay, increase patient receipt of early physical therapy, decrease driveline infection incidence, and improve nutritional status. With 2008 as our baseline, we deployed interventions for each outcome area across 2009 to 2017. Performance improvement activities included staff, patient, and family didactic, one-on-one, and hands-on education; procedural changes; and outcomes monitoring with feedback to staff on progress. Descriptive and inferential statistics were examined to document change in the outcomes. Outcomes: Across the performance improvement period, length of stay decreased from 40 to 23 days; physical therapy consults increased from 87% to 100% of patients; 1-year driveline infection incidence went from 38% to 23.5%; and the percentage of patients with prealbumin within the normal range increased from 84% to 90%. Implications: Performance improvement interventions may enhance ventricular assist device patient outcomes. Interventions’ sustainability should be evaluated to ensure that gains are not lost over time.

Die digitale revolusie tydens die laaste drie dekades het ’n enorme impak gemaak op die wyse waarop verbruikers en verskaffers tans optree. Hierdie ontwikkeling skep nou nuwe uitdagings vir verbruikersbeskerming en die regulering van verskaffers. Die verbruikersregbeskermingsmodel waarop bestaande verbruikersregwetgewing in Suid-Afrika en wêreldwyd geskoei is, moet opnuut in oënskou geneem word om te bepaal of dit nog in pas is met die eise van die internetverbruikerswêreld. Die artikel skop af met ’n ontleding van die eienskappe van digitale verbruikers en die soort transaksies wat hulle sluit. Daar word ook daarop gewys hoe verskillende veilige betalingsmetodes bygedra het om die vertroue van verbruikers in internethandel te laat groei. Die artikel ontleed die bestaande pogings om verbruikersreg wêreldwyd te harmonieer. Ten spyte van kulturele, sosiale en ekonomiese verskille bestaan daar nogtans ’n wye verskeidenheid van soortgelyke kwessies wat voorkom en wat vatbaar is vir harmoniëring. Dit is veral die geval met betrekking tot die kwessies wat internethandel opwerp. Landsgrense is vinnig besig om te verdwyn en oorgrensverbruikerstransaksies is nou alledaags wat verdere eise aan verbruikersbeskerming stel. Die invloed van die Verenigde Volke se Kommissie vir Internasionale Handelsreg (UNCITRAL) se Modelwet vir Elektroniese Handel en Transaksies (Model Law on Electronic Commerce) van 1996 op die Suid-Afrikaanse reg en verbruikersreg word ontleed. Daar word ook gekyk of die Wet op Elektroniese Kommunikasie en Transaksies 25 van 2002 wat op die Model Law geskoei is nog pasgemaak is om meer onlangse ontwikkelings baas te raak. Daar word ook ’n ontleding gedoen van die wisselwerking tussen die Wet op Elektroniese Kommunikasie en Transaksies 25 van 2002 en die latere Verbruikersbeskermingswet (Consumer Protection Act) 68 van 2008. Die artikel toon hoe die groei van toegang tot slimfone in Afrika tot wyer toegang tot die internet aanleiding gee en daarmee bydra om die sogenaamde digitale gaping te vernou. Dit maak dit nou ook moontlik vir verbruikers in afgeleë landelike gebiede om deel te neem aan internethandel. Daar word verwys na die sukses met die elektroniese bankwese in Oos-Afrika in hierdie proses. Die ontwikkeling van verbruikersreg in Suid-Afrika word as ’n voorbeeld gebruik om aan te toon hoe die plaaslike verbruikersreg wat tot relatief onlangs onderontwikkel was, deur omvattende wetgewing soos die Nasionale Kredietwet 34 van 2005, die Consumer Protection Act (Verbruikersbeskermingswet wat egter nie op Afrikaans uitgegee is nie) 68 van 2008 en die Wet op Beskerming van Persoonlike Inligting 4 van 2013 vernuwe is, maar dat hierdie wetgewing alreeds nie meer voldoende voorsiening maak vir die eise van internethandel nie. Ten slotte word daar breedweg gewys op die vereistes waaraan verbruikersbeskermingswetgewing moet voldoen om voorsiening te maak vir digitale verbruikersbeskerming. Daar word ook aan die hand gedoen dat die feit dat baie Afrika lande se verbruikersbeskermingswetgewing nog onderontwikkeld is, die moontlikheid bied om moderne pasgemaakte maatreëls te ontwikkel met verwysing na verwikkelinge elders in die wêreld sonder om deur die langsame ontwikkelingsprosesse van elders te gaan.

En este artículo se presenta el Enfoque de los Itinerarios de Enseñanza de las Matemáticas, un enfoque que trata de ser respetuoso con las necesidades reales de los estudiantes para aprender matemáticas. En la primera parte se presenta la fundamentación del enfoque, que se sustenta en tres pilares interrelacionados: la perspectiva sociocultural del aprendizaje humano, el modelo de formación realista-reflexivo y la educación matemática realista; en la segunda parte se describe el enfoque, que se refiere a una secuencia de enseñanza intencionada que contempla tres niveles: 1) enseñanza en contextos informales (el entorno inmediato, los materiales manipulativos y los juegos); 2) enseñanza en contextos intermedios (recursos literarios y tecnológicos), y 3) enseñanza en contextos formales (recursos gráficos); finalmente, en la tercera parte se ejemplifica dicho enfoque con un itinerario de enseñanza del álgebra temprana para estudiantes de 3 a 12 años. Se concluye que la implementación de este enfoque requiere un amplio dominio de conocimientos didáctico-disciplinares, lo que implica un esfuerzo importante por parte de todos los agentes implicados en la formación del profesorado para que así, todo aquel profesional preocupado por mejorar su práctica docente y adaptarla a las exigencias del siglo XXI, pueda tener acceso a estos conocimientos. Referencias Alsina, Á. (2004). Barrinem? Matemàtiques amb jocs i problemes. Lògica 3. Cataluña: Edicions l'Àlber, S.L. Alsina, Á. (2010). La “pirámide de la educación matemática”, una herramienta para ayudar a desarrollar la competencia matemática. Aula de Innovación Educativa, 189, 12-16. Recuperado desde https://dugi-doc.udg.edu//bitstream/handle/10256/9481/PiramideEducacion.pdf Alsina, Á. (2018). Seis lecciones de educación matemática en tiempos de cambio: itinerarios didácticos para aprender más y mejor. Padres y Maestros, 376, 13-20. Alsina, Á. (2019a). 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At the origin of the International Law Department were such eminent scientists, diplomats and teachers as V.N. Durdenevsky, S.B. Krylov and F.I. Kozhevnikov. International law studies in USSR and Russia during the second half of the XX century was largely shaped by the lawyers of MGIMO. They had a large influence on the education in the international law in the whole USSR, and since 1990s in Russia and other CIS countries. The prominence of the research of MGIMO international lawyers was due to the close connections with the international practice, involving international negotiations in the United Nations and other international fora, diplomatic conferences and international scientific conferences. This experience is represented in the MGIMO handbooks on international law, which are still in demand. The Faculty of International Law at MGIMO consists of seven departments: Department of International Law, Department of Private International and Comparative Law; Department of European Law; Department of Comparative Constitutional Law; Department of Administrative and Financial Law; Department of Criminal Law, Department Criminal Procedure and Criminalistics. Many Russian lawyers famous at home and abroad work at the Faculty, contributing to domestic and international law studies. In 1947 the Academy of Sciences of the USSR published "International Law" textbook which was the first textbook on the subject in USSR. S.B. Krylov and V.N. Durdenevsky were the authors and editors of the textbook. First generations of MGIMO students studied international law according to this textbook. All subsequent books on international law, published in the USSR, were based on the approach to the teaching of international law, developed in the textbook by S.B. Krylov and V.N. Durdenevsky. The first textbook of international law with the stamp of MGIMO, edited by F.I. Kozhevnikov, was published in 1964. This textbook later went through five editions in 1966, 1972, 1981, 1987. In 1994 the International Law Department together with the Diplomatic Academy of the Russian Ministry of Foreign Affairs prepared new textbook, reflecting the development of international law in the 1960-1990s. In 2000 "International Law" textbook appeared, which was prepared exceptionally by the Department of International Law at MGIMO. In 2005 "European international law" textbook was published. It became the first textbook in Russian Law studies dedicated to the international legal aspects of interstate cooperation in Europe. Quarterly magazine "Moscow Journal of International Law" has made significant contribution to the development of the MGIMO international law school. Y.M. Kolosov, who is the Honored Scientist of Russia and professor of international law, was the founder of the magazine. He has been its editor in chief up to present.

Specimens of Trichodina magna Van As and Bassson, 1989 (Ciliophora: Peritrichia) from the Nile tilapia Oreochromis niloticus collected from October 2004 to June 2005 in fish ponds situated in three regions of Santa Catarina State, Brazil are described here. Wet smears of skin and gills were prepared in the field, air dried, impregnated with Klein’s dry silver method and Giemsa’s solution. From a total of 146 examined fish, 36 were parasitized on the skin, 14 in the gills and 33 on the skin and gills, simultaneously. The mean diameter of the body of the specimens of T. magna was 84.3 ± 12.6 µm, adhesive disc 60.7 ± 10.0 µm, denticulate ring, 38.3 ± 7.4 µm, consisting of 26 (23 to 29) denticles. The only distinguishable difference from the original description was the fact that the ray of the denticle is anteriorly directed and does not extend over the y + 1 axis.

Abstract. Gas and aerosol samples were taken using a wet effluent diffusion denuder/aerosol collector (WEDD/AC) coupled to ion chromatography (IC) in the city of Zurich, Switzerland from August to September 2002 and in March 2003. Major water soluble inorganic ions; nitrate, sulfate, and nitrite were analyzed online with a time resolution of two hours for the gas and aerosol phase. The fraction of water soluble inorganic anions in PM10 varied from 15% in August to about 38% in March. Seasonal and diurnal variations of nitrate in the gas and aerosol phase were observed with more than 50% of the total nitrate in the gas phase during August and more than 80% of nitrate in the aerosol phase during March exceeding the concentration of sulfate by a factor of 2. Aerosol sulfate, on the other hand, did not show significant variability with season. However, in the gas phase, the SO2 concentration was 6.5 times higher in winter than in summer. Nitrous acid (HONO) also showed a diurnal variation in both the gas and aerosol phase with the lowest concentration (0.2–0.6 µg/m3) in the afternoon. The primary pollutants, NO, CO and SO2 mixing ratios were often at their highest between 04:00–10:00 local time due to the build up of fresh vehicle emission under a nocturnal inversion.

Abstract The “Optimal Liver Iron” range in iron overloaded patients with thalassemia has been established by Olivieri and Brittenham (Blood, 1997) as 1–2.2 mg/g wet weight liver using direct measurement or calculated as 3.2 to 7 mg/g dry liver weight based on a 3.33 correction factor for body water. This report describes the relationship between liver iron concentration (LIC) measured by SQUID biosusceptometry (BLS, wet weight) compared to liver biopsy (dry weight), in patients with thalassemia (THAL) or sickle cell disease (SCD). A total of 38 chronically transfused patients (THAL n=19, SCD n=19) were prospectively assessed for LIC measured by BLS and liver biopsy within 2 month of each other. Most BLS results were submitted to data repository before receiving iron concentration from liver biopsy. LIC was measured by a low temperature SQUID biosusceptometer system (Ferritometer®) under the standardized Hamburg-Torino-Oakland protocol. Iron in fresh tissue and paraffin embedded liver biopsy samples was measured at Mayo clinics by Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Subjects ranged in age from 5 to 40 years (median: 18 y). Median LIC (n=38) measured by SQUID was 1777 (365–4883) [mg/g wet weight], median LIC by biopsy (fresh tissue) was 10861(1854–32864) [mg/g dry weight]. After excluding the subjects with BMI&gt;30 kg/m2 (n=5), the Spearman rank correlation between wet weight LIC assessed by BLS and dry weight LIC from fresh tissue sample measured by ICP-MS was highly significant (RS=0.90, p&lt;0.0001). No significant difference between fresh tissue and paraffin embedded LIC values was observed. The agreement between BLS and fresh tissue biopsy was tested by Bland-Altman plots. After normalization, no significant proportional or zero bias was visible. The 95% limits of agreement between SQUID-BLS and biopsy LIC values were found between −56% (or –6733 μg/g) and 48% (or 7027 μg/g). This kind of agreement is similar to the results achieved with MRI-R2 by St. Pierre et al (Blood, 2005)(–56% and 50%), however, BLS is a totally independent method relying only on the specific magnetic susceptibility of the hemosiderin-ferritin iron complex. The conversion factor between the two methods (LIC by fresh tissue biopsy vs. LIC by BLS) obtained from a weighted linear regression with zero intercept (Marquardt algorithm) was 6.1 ± 0.3 (R2 = 0.86–0.88, cn2 = 2.3–2.6). Using this conversion factor, the recommended “optimal range” for liver iron in patients with thalassemia major should be considered as 6–13.2 mg/g dry weight liver rather than the generally accepted 3.2–7 mg/g dry weight liver.

Abstract Background Daptomycin (dap) has been approved and successfully used for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. However, reports of daptomycin nonsusceptible (DNS) MRSA strains have emerged over the recent years. This study describes the clinical characteristics of patients with DNS MRSA bloodstream infections (BSIs) with the objective of identifying risk factors and outcomes. Methods This is a retrospective case–control study in a tertiary healthcare system in southeast Michigan. Cases included 34 patients with DNS MRSA BSI between September 24, 2005 and March 31, 2018. Cases were matched with controls with MRSA BSI based on age, source of BSI, and time-period of BSI in a 1:1 ratio. Charts were reviewed for clinical and laboratory data. Vancomycin (van) and dap minimum inhibitory concentrations (MICs) were determined by E-test. DNS was defined as an MIC &gt;1.0 µg/mL. Chi-square test, Fisher’s exact test, and t-test were used to determine statistical significance. Results In the case cohort, the source of BSI was endovascular in 11(32%) patients, central-line associated in 3(9%), secondary BSI in 13(38%), and unknown in 7(21%). Table 1 is a summary of the results. Conclusion Prior exposure to dap and van, and higher van MIC in MRSA isolates are risk factors for DNS MRSA BSI. DNS is associated with significantly higher risk of 90-day MRSA BSI recurrence. Disclosures All authors: No reported disclosures.

Objective.Psoriatic arthritis (PsA) is characterized by varied radiographic features. We describe a patient with PsA with severe radiographic damage that improved significantly following treatment with etanercept. The improvement was documented by several methods of radiographic assessment.Methods.Etanercept was introduced in September 2005. Radiographs of the hands and feet were read using 3 methods: the modified Steinbrocker method, the van der Heijde (vdH) modification of the Sharp method, and the Ratingen scoring system.Results.In July 2009, radiographs of the hands and feet showed improvement in erosion score and joint space narrowing, while bony proliferation remained the same [43 by modified Steinbrocker, 26 by the vdH Sharp score (12 for erosions and 14 for joint space narrowing), and 56 by the Ratingen (18 for erosion and 38 for proliferation].Conclusion.The 3 radiographic methods were useful in demonstrating improvement in joint scores. The modified Steinbrocker method, which is the simplest, was able to reveal improvement in our patient.

Many approaches have been applied to forecast climate using statistical and deterministic models using independent and dependent variables empirically. It is more practical to analyze the parameters, but it needs validation anytime and anywhere. Kalman filtering unites physical and statistical model approaches to stochastic model renewable anytime for objective of on line forecasting. Based on research, sea surface temperature Nino 3.4 have high correlation with rainfall in Indonesia, so it is used to forecast rainfall in Cirebon as area study. Rainfall clustering in Cirebon results 6 groups with rainfall average 1400-1500 mm/year for dry area and 3000-3200 mm/year for wet area. Validation have correlation coefficient validation value more than 94%, correlation coefficient model value more than 78% and fit model value more than 38%. The result of regression gives R2 value of more than 0,8. It implies that predicting model using Kalman Filter is feasible to forecast montly rainfall based on sea surface temperature Nino 3.4. The result of rainfall prediction in Cirebon show increasing in rainfall until February 2005, with correlation coeficient value of model more than 90% and fit model more than 40%.

Abstract Abstract 1223 Poster Board I-245 Introduction This study aimed at evaluating the impact of three different pre-transplant therapies on the outcome of patients (pts) eligible for high-dose therapy. Methods two-hundred sixty eight newly diagnosed MM pts aged £65 years, Durie-Salmon stage III, II, or I in progression, were consecutively enrolled from 2000 to 2007 in three different protocols, with three different pre-transplant therapy: Group 1: (145 pts) 3 pulse-VAD cycles; Group 2: (67 pts) 3 pulse-VAD cycles plus 3 Thal-Dex cycles (thalidomide at the dose of 100 mg/day orally at bedtime, continuously for 3 months, oral dexamethasone at the dose of 20 mg on days 1-4 and 14-17 every 28 days); Group 3: (57pts) 4 Vel-Dex courses (Bortezomib 1.3 mg/m2 i.v. on days 1, 4, 8, 11; oral Dexamethasone 40 mg on days 1-4 and 8-11 every 3 weeks). After induction all pts received two DCEP-short cycles as mobilization (oral Dexamethasone 40 mg/day on days 1-4 + Cyclophosphamide 700 mg/m2/day i.v., Etoposide 100 mg/ m2/day i.v., cisPlatin 25 mg/m2/day for 2 days) with peripheral blood stem-cell (PBSC) collection prompted by G-CSF followed by one or two transplants (Tx) with melphalan 200 mg/m2 as conditioning regimen. Response was defined according to IMWG uniform criteria. Pts were considered responsive when obtaining at least a PR. Results pts in the three group were similar for age, gender, Ig type, ISS stage. A significant higher percentage of Durie and Salmon stages III was found in group 3 (83% vs 68% in group 1 and 67% in group 2, p=0.0002). The median follow-up was 46 (1-150) months for group 1, 43 (1-68) months for group 2, and 29.7 (1-79) months for group 3. At the time of this analysis in the three groups 51%, 65%, 90% of transplanted pts respectively were still alive, and progression after transplant was registered in 84%, 80%, 50% respectively. Patient flow before Tx was similar (p=0.45): 19% in group 1, 27% in group 2, 23% in group 3. In group 1, 2% of pts went off-study after VAD, and 17% after mobilization phase. In group 2, patient flow was equally distributed: 7% after pulse VAD, 10% after thal-dex, 9% after DCEP. In group 3, 12% of the pts went off-study after Vel-Dex, 11% after DCEP. Table 1 summarized responses. In group 3 (Vel-Dex) response was better along all protocol phases with respect to group 1 or 2 (p<0.00001). The number of responsive pts progressively increased from 87% after Vel-Dex (CR 31%), to 96% after transplant (CR 38%). Response rates of group 1 and 2 patients were not significantly different either after induction (p=0.6), after DCEP (p=0.5), and after Tx (p=0.65). On intention to treat basis, vel-dex induction produced a better, although not significant, PFS (34.6 months vs 29 in group 1 and 26.8 in group 2, p=0.56). OS were not statistically different among the three groups, event though the different follow-up could affect the analysis (median OS 110 in group 1, 66 months in group 2, and not reached in group 3, p=0.37). In multivariate analysis PFS was improved only by the achievement of CR (p=0.001). No significant difference was observed between VGPR or PR (p=0.43). Conclusion In this study, only CR not VGPR impacts on the outcome. Vel-Dex producing a significant high CR rate after TX (38%), seems to improve survival of MM patients candidate to high-dose therapy with respect to conventional pre-transplant strategies. Disclosures Morra: Roche:.

The aim of this paper was to investigate the potential of zearalenone (ZEN) and type A trichothecenes (T-2 toxin and diacetoxyscirpenole - DAS) for biosynthesis by Fusarium spp. isolated from corn kernels contaminated by mycotoxins. The samples of corn kernels (2004 and 2005 harvest) originating in different regions of Backa (Vojvodina, Serbia) were tested. Mycotoxicological investigations showed in most cases a significant contamination with deoxynivalenol (DON 200 - 2,460 ?g/kg) and ZEA (520 1,680 ?g/kg). Isolations and identifications of fusaria established only the presence of species F. verticillioides, after one month storage in freezer conditions, in fusariotoxin positive samples. The control cultures known as ZEA producers - F. graminearum GZ-LES, i.e. T-2 toxin and DAS producer - F. sporotrichioides KF-38/1/R were also tested. In vitro toxicological investigations of isolated fusaria were performed in liquid semisynthetic media (GPK or SPK), and on wet sterilized corn kernels, respectively. Under testing conditions, analyzed F. verticillioide and F. sporotrishioides isolates were not ZEA producers. Contrary to them, F. graminearum GZ-LES pure culture was very good producer of fusariotoxins; it biosynthesized max. 465,900 ?g/kg DON, and 4,416 ?g/kg ZEA, respectively. Cultivation conditions influenced a great deal of T-2 toxin production under laboratory conditions. In most cases, higher yields were obtained during the cultivation of F. verticillioides in liquid glucose medium (80-240 ?g/L). Contrary to the control strain F. sporotrichioides KF-38/1/R that under the same conditions synthesized, besides T-2 toxin (4.000 ?g/L) and DAS (240 ?g/L), isolates of F. verticillioides from corn grain did not show that ability.

Abstract. Sublimation plays a decisive role in the surface energy balance of tropical glaciers. During the dry season low specific humidity and high surface roughness favour the direct transition from ice to vapour and drastically reduce the energy available for melting. However, field measurements are scarce and little is known about the performance of sublimation parametrisations in glacier mass balance and runoff models. During 15 days in August 2005 sublimation was measured on the tongue of Glaciar Artesonraju (8°58' S, 77°38' W) in the Cordillera Blanca, Perú, using simple lysimeters. Indicating a strong dependence on surface roughness, daily totals of sublimation range from 1–3 kg m−2 for smooth to 2–5 kg m−2 for rough conditions. Measured sublimation was related to characteristic surface roughness lengths for momentum (zm) and for the scalar quantities of temperature and water vapour (zs), using a process-based mass balance model. Input data were provided by automatic weather stations, situated on the glacier tongue at 4750 m ASL and 4810 m ASL, respectively. Under smooth conditions the combination zm=2.0 mm and zs=1.0 mm appeared to be most appropriate, for rough conditions zm=20.0 mm and zs=10.0 mm fitted best. Extending the sublimation record from April 2004 to December 2005 with the process-based model confirms, that sublimation shows a clear seasonality. 60–90% of the energy available for ablation is consumed by sublimation in the dry season, but only 10–15% in the wet season. The findings are finally used to evaluate the parametrisation of sublimation in the lower-complexity mass balance model ITGG, which has the advantage of requiring precipitation and air temperature as only input data. It turns out that the implementation of mean wind speed is a possible improvement for the representation of sublimation in the ITGG model.

Absztrakt A gyomor primer lymphomáinak kezelési stratégiája napjainkban a hatékony kemoterápiának és immunoterápiának köszönhetően változás alatt áll. Míg korábban az elsődleges gyógymód a sebészi reszekció volt és a kemoterápia csak mint ennek kiegészítése jöhetett szóba kuratív célból, addig ma ez a trend megfordulni látszik. A korai stádiumú kis malignitású MALT lymphoma – ha a beteg Helicobacter pozitív – 60%-ban meggyógyítható eradikációs kezeléssel. Totalis gastrectomiát vagy szubtotalis reszekciót D2 lymphadenectomiával II.1. stádiumig javaslunk, R1 esetben adjuváns kemoterápiával. Nagy malignitású gyomor lymphoma esetén a stratégia hasonló, azzal a kiegészítéssel, hogy II.1. stádiumban csak akkor van értelme a reszekciónak, ha biztosan R0 status érhető el. Ezeken kívül kemorezisztens betegeknél is indokolt a reszekció. Az előrehaladott esetekben a műtétnek nincs értelme, csak a szövődmények elhárítása lehet a cél. Sajnos a diagnosztikai nehézségek miatt gyakran csak posztoperatíve derül ki, hogy lymphomát operáltunk. Jelen munkánkban 2000. január 1. és 2005. december 31. közötti 6 éves periódusban kezelt 38 primer gyomorlymphomás betegünk adatait dolgoztuk fel. Összesen 9 beteg került műtétre, reszekciós műtét 6 esetben történt. Műtét előtt csak 1 betegnél tudtuk, hogy lymphomát operálunk. A tendencia az, hogy a kemoterápia, immuno-kemoterápia egyre nagyobb teret nyer a gyomor lymphomák gyógyításában. Saját anyagunk is ezt a változást tükrözi.

The problem of the coating of steel has been considered in several Mathematics in Industry study groups. In this process, after passing through a bath of molten alloy, steel sheeting is drawn upward to allow draining under gravity and stripping using an air knife, leaving a coating of desirable thickness. Here we discuss some aspects of the problem and in particular the gravity draining component. The problem is a very nice introduction to industrial modelling for students, but is also relevant for manufacturing. References Elsaadawy, E. A., Hanumanth, G. S., Balthazaar, A. K. S., McDermid, J. R., Hrymak, A. N. and Forbes, J.F. ``Coating weight model for the continuous hot-dip galvanizing process'', Metal. Mat. Trans. B, 38:413–424, 2007. doi:10.1007/s11663-007-9037-2 Hocking, G. C., Sweatman, W. L., Fitt, A. D., and Roberts M. ``Coating Deformation in the jet stripping process'' in Proceedings of the 2009 Mathematics and Statistics in Industry Study Group, Eds. T. Marchant, M. Edwards, G. Mercer. Wollongong, Austealia, 2010. https://documents.uow.edu.au/content/groups/public/@web/@inf/@math/documents/doc/uow073330.pdf Hocking, G. C., Sweatman, W. L., Fitt, A. D., and Breward, C. ``Deformations arising during air-knife stripping in the galvanization of steel'', in Progress in Industrial Mathematics at ECMI 2010, Eds. M. Gunther, A. Bartel, M. Brunk, S. Schops, M. Striebel. Mathematics in Industry 17, pp. 311-317. Springer, Berlin Heidelberg, 2011. doi:10.1007/978-3-642-25100-9_36 Hocking, G. C., Lavalle, G., Novakovic, R., O'Kiely, D., Thomson, S., Mitchell, S. J., Herterich, R. ``Bananas–-defects in the jet stripping process''. Proceedings of the European Study Group with Industry in Mathematics and Statistics Research Collection. Rome Italy, 2016. https://researchrepository.ucd.ie/handle/10197/10215 Howison, S. D. and King, J. R. ``Explicit solutions to six free-boundary problems to fluid flow and diffusion''. IMA J. Appl. Math. 42:155–175, 1989. doi:10.1093/imamat/42.2.155 Hocking, G. C., Sweatman, W., Fitt, A. D. and Breward, C. ``Deformations during jet-stripping in the galvanizing process''. J. Eng. Math. Tuck Special Issue, 70:297–306, 2011. doi:10.1007/s10665-010-9394-8 Thornton, J. A. and Graff, H. F. ``An analytical description of the jet-finishing process for hot-dip metallic coatings on strip''. Metal. Mat. Trans. B, 7:607–618, 1976. doi:10.1007/BF02698594 Tuck, E. O. ``Continuous coating with gravity and jet stripping''. Phys. Fluids, 26(9):2352–2358, 1983. doi:10.1063/1.864438 Tuck, E. O., Bentwich, M., and van der Hoek, J. ``The free boundary problem for gravity-driven unidirectional viscous flows''. IMA J. Appl. Math. 30:191–208, 1983. doi:10.1093/imamat/30.2.191

Abstract. Sublimation plays a decisive role in the surface energy and mass balance of tropical glaciers. During the dry season (May–September) low specific humidity and high surface roughness favour the direct transition from ice to vapour and drastically reduce the energy available for melting. However, field measurements are scarce and little is known about the performance of sublimation parameterisations in glacier mass balance and runoff models. During 15 days in August 2005 sublimation was measured on the tongue of Glaciar Artesonraju (8°58' S, 77°38' W) in the Cordillera Blanca, Perú, using simple lysimeters. Indicating a strong dependence on surface roughness, daily totals of sublimation range from 1–3 kg m−2 for smooth to 2–5 kg m−2 for rough conditions. (The 15-day means at that time of wind speed and specific humidity were 4.3 m s−1 and 3.8 g kg−1, respectively.) Measured sublimation was related to characteristic surface roughness lengths for momentum (zm) and for the scalar quantities of temperature and water vapour (zs), using a process-based mass balance model. Input data were provided by automatic weather stations, situated on the glacier tongue at 4750 m a.s.l. and 4810 m a.s.l., respectively. Under smooth conditions the combination zm=2.0 mm and zs=1.0 mm appeared to be most appropriate, for rough conditions zm=20.0 mm and zs=10.0 mm fitted best. Extending the sublimation record from April 2004 to December 2005 with the process-based model confirms, that sublimation shows a clear seasonality. 60–90% of the energy available for ablation is consumed by sublimation in the dry season, but only 10–15% in the wet season (October–April). The findings are finally used to evaluate the parameterisation of sublimation in the lower-complexity mass balance model ITGG, which has the advantage of requiring precipitation and air temperature as only input data. It turns out that the implementation of mean wind speed is a possible improvement for the representation of sublimation in the ITGG model.

<p><strong>List of abstracts and authors:</strong></p><p><strong>1. Bipolar disorder not otherwise specified -overdiagnosed or underdiagnosed?</strong></p><p>E Allers</p><p><strong>2. The prognosis of major depression untreated and treated: Does the data reflect the true picture of the prognosis of this very common disorder?</strong></p><p>E Allers</p><p><strong>3. Can we prolong our patients' life expectancy? Providing a better quality of life for patients with severe mental illness</strong></p><p>O A Betencourt</p><p><strong>4. The scope of ECT practice in South Africa</strong></p><p>J Benson-Martin, P Milligan</p><p><strong>5. Biomarkers for schizophrenia: Can we evolve like cancer therapeutics?</strong></p><p>P Buckley<strong></strong></p><p><strong>6. Relapse in schizophrenis: Major challenges in prediction and prevention</strong></p><p>P Buckley</p><p><strong>7. Informed consent in biological treatments: The right to know the duty to inform</strong></p><p><strong></strong>I Chetty</p><p><strong>8. Effectiveness of a long-acting injectable antipsychotic plus an assertive monitoring programme in first-episode schizophrenia</strong></p><p><strong></strong>B Chiliza, L Asmal, O Esan, A Ojagbemi, O Gureje, R Emsley</p><p><strong>9. Name, shame, fame</strong></p><p>P Cilliers</p><p><strong>10. Can we manage the increasing incidence of violent raging children? We have to!</strong></p><p>H Clark</p><p><strong>11. Serotonin, depression and antidepressant action</strong></p><p>P Cowen</p><p><strong>12. Prevalence and correlates of comorbid psychiatris illness in patients with heroin use disorder admitted to Stikland Opioid Detoxification Unit</strong></p><p>L Dannatt, K J Cloete, M Kidd, L Weich</p><p><strong>13. Investigating the association between diabetes mellitus, depression and psychological distress in a cohort of South African teachers</strong></p><p>A K Domingo, S Seedat, T M Esterhuizen, C Laurence, J Volmink, L Asmal</p><p><strong>14. Neuropeptide S -emerging evidence for a role in anxiety</strong></p><p>K Domschke</p><p><strong>15. Pathogenetics of anxiety</strong></p><p>K Domschke</p><p><strong>16. The effects of HIV on the fronto-striatal system</strong></p><p>S du Plessis, M Vink, J Joska, E Koutsilieri, C Scheller, B Spottiswoode, D Stein, R Emsley</p><p><strong>17. Effects of acute antipsychotic treatment on brain morphology in schizophrenia</strong></p><p>R Emsley, L Asmal, B Chiliza, S du Plessis, J Carr, A Goosen, M Kidd, M Vink, R Kahn</p><p><strong>18. Development of a genetic database resource for monitoring of breast cancer patients at risk of physical and psychological complications</strong></p><p>K Grant, F J Cronje, K Botha, J P Apffelstaedt, M J Kotze</p><p><strong>19. Unipolar mania reconsidered: Evidence from a South African study</strong></p><p><strong></strong>C Grobler</p><p><strong>20. Antipsychotic-induced movement disorders: Occurence and management</strong></p><p>P Haddad</p><p><strong>21. The place of observational studies in assessing the effectiveness of long-acting injectable antipsychotics</strong></p><p>P Haddad</p><p><strong>22. Molecular mechanisms of d-cycloserine in fear extinction: Insights from RNS sequencing</strong></p><p>S Hemmings, S Malan-Muller, L Fairbairn, M Jalali, E J Oakeley, J Gamieldien, M Kidd, S Seedat</p><p><strong>23. Schizophrenia: The role of inflammation</strong></p><p>DC Henderson</p><p><strong>24. Addictions: Emergent trends and innovations</strong></p><p>V Hitzeroth</p><p><strong>25. The socio-cultural-religious context of biological psychiatric practice</strong></p><p>B Janse van Rensburg</p><p><strong>26. Biochemical markers for identifying risk factors for disability progression in multiple sclerosis</strong></p><p><strong></strong>S Janse van Rensburg, M J Kotze, F J Cronje, W Davis, K Moremi, M Jalali Sefid Dashti, J Gamieldien, D Geiger, M Rensburg, R van Toorn, M J de Klerk, G M Hon, T Matsha, S Hassan, R T Erasmus</p><p><strong>27. Alcohol-induced psychotic disorder: Brain perfusion and psychopathology - before and after antipsychotic treatment</strong></p><p>G Jordaan, J M Warwick, D G Nel, R Hewlett, R Emsley</p><p><strong>28.'Pump and dump': Harm reduction strategies for breastfeeding while using substances</strong></p><p>L Kramer</p><p><strong>29. Adolescent neuropsychiatry - an emerging field in South African adolescent psychiatric services</strong></p><p>A Lachman</p><p><strong>30. Recovery versus remission, or what it means to be healthy for a psychiatric patient?</strong></p><p>B Latecki</p><p><strong>31. Holistic methods utilised to normalise behaviours in youth diagnosed with neuro-biochemical disorders</strong></p><p>P Macqueen</p><p><strong>32. Candidate genes and novel polymorphisms for anxiety disorder in a South African cohort</strong></p><p>N McGregor, J Dimatelis, S M J Hemmings, C J Kinnear, D Stein, V Russel, C Lochner</p><p><strong>33. Higher visual functioning</strong></p><p>A Moodley</p><p><strong>34. The effects of prenatal methylmercury exposure on trace element and antioxidant levels in rat offspring following 6-hydroxydopamine-induced neuronal insult</strong></p><p>Z M Moosa, W M U Daniels, M V Mabandla</p><p><strong>35. Paediatric neuropsychiatric movement disorders</strong></p><p>L Mubaiwa</p><p><strong>36. The South African national female offenders study</strong></p><p>M Nagdee, L Artz, C de Clercq, P de Wet, H Erlacher, S Kaliski, C Kotze, L Kowalski, J Naidoo, S Naidoo, J Pretorius, M Roffey, F Sokudela, U Subramaney</p><p><strong>37. Neurobiological consequences of child abuse</strong></p><p>C Nemeroff</p><p><strong>38. What do Stellenbosch Unviversity medical students think about psychiatry - and why should we care?</strong></p><p>G Nortje, S Suliman, K Seed, G Lydall, S Seedat</p><p><strong>39. Neurological soft skins in Nigerian Africans with first episode schizophrenia: Factor structure and clinical correlates</strong></p><p><strong></strong>A Ojagbemi, O Esan, O Gureje, R Emsley</p><p><strong>40. Should psychiatric patients know their MTHFR status?</strong></p><p>E Peter</p><p><strong>41. Clinical and functional outcome of treatment refractory first-episode schizophrenia</strong></p><p>L Phahladira, R Emsley, L Asmal, B Chiliza</p><p><strong>42. Bioethics by case discussion</strong></p><p>W Pienaar</p><p><strong>43. Reviewing our social contract pertaining to psychiatric research in children, research in developing countries and distributive justice in pharmacy</strong></p><p>W Pienaar</p><p><strong>44. The performance of the MMSE in a heterogenous elderly South African population</strong></p><p>S Ramlall, J Chipps, A I Bhigjee, B J Pillay</p><p><strong>45. Biological basis addiction (alocohol and drug addiction)</strong></p><p>S Rataemane</p><p><strong>46. Volumetric brain changes in prenatal methamphetamine-exposed children compared with healthy unexposed controls</strong></p><p><strong></strong>A Roos, K Donald, G Jones, D J Stein</p><p><strong>47. Single voxel proton magnetic resonance spectroscopy of the amygdala in social anxiety disorder in the context of early developmental trauma</strong></p><p>D Rosenstein, A Hess, S Seedat, E Meintjies</p><p><strong>48. Discussion of HDAC inhibitors, with specific reference to supliride and its use during breastfeeding</strong></p><p>J Roux</p><p><strong>49. Prevalence and clinical correlates of police contact prior to a first diagnosis of schizophrenia</strong></p><p>C Schumann, L Asmal, K Cloete, B Chiliza, R Emsley</p><p><strong>50. Are dreams meaningless?</strong></p><p>M Solms</p><p><strong>51. The conscious id</strong></p><p>M Solms<strong></strong></p><p><strong>52. Depression and resilience in HIV-infected women with early life stress: Does trauma play a mediating role?</strong></p><p>G Spies, S Seedat</p><p><strong>53. State of affairs analysis for forensic psychiatry in SA</strong></p><p>U Subramaney</p><p><strong>54. Escitalopram in the prevention of post-traumatic stress disorder: A pilot randomised controlled trial</strong></p><p>S Suliman, S Seedat, J Pingo, T Sutherland, J Zohar, D J Stein</p><p><strong>55. Epigenetic consequences of adverse early social experiences in primates</strong></p><p>S Suomi</p><p><strong>56. Risk, resilience, and gene x environment interactions in primates</strong></p><p>S Suomi</p><p><strong>57. Biological aspects of anorexia nervosa</strong></p><p>C Szabo</p><p><strong>58. Agents used and profiles of non-fatal suicidal behaviour in East London</strong></p><p>H Uys</p><p><strong>59. The contributions of G-protein coupled receptor signalling to opioid dependence</strong></p><p>J van Tonder</p><p><strong>60. Emerging trend and innovation in PTSD and OCD</strong></p><p>J Zohar</p><p><strong>61. Making the SASOP treatment guidelines operational</strong></p><p>E Allers</p><p><strong>Poster Presentations</strong></p><p><strong>62. Neuropsychological deficits in social anxiety disorder in the context of early developmental trauma</strong></p><p><strong></strong>S Bakelaar, D Rosenstein, S Seedat</p><p><strong>63.Social anxiety disorder in patients with or without early childhood trauma: Relationship to behavioral inhibition and activation and quality of life</strong></p><p><strong></strong>S Bakelaar, C Bruijnen, A Sambeth, S Seedat</p><p><strong>64. Exploring altered affective processing in obssessive compulsive disorder symptom subtypes</strong></p><p>E Breet, J Ipser, D Stein, C Lochner<strong><br /></strong></p><p><strong>65. To investigate the bias toward recognising the facial expression of disgust in obsessive compulsive disorder as well as the effect of escitalopram</strong></p><p>E Breet, J Ipser, D Stein, C Lochner</p><p><strong>66. A fatal-case of nevirapine-induced Stevens-Johnson's syndrome in HIV mania</strong></p><p>A Bronkhorst, Z Zingela, W M Qwesha, B P Magigaba<strong></strong></p><p><strong>67. Association of the COMT G472A (met/met) genotype with lower disability in people diagnosed with multiple sclerosis</strong></p><p>W Davis, S J van Rensburg, L Fisher, F J Cronje, D Geiger, M J Kotze</p><p><strong>68. Homocycsteine levels are associated with the fat mass and obesity associated gene FTO(intron 1 T&gt;A) polymorphism in MS patients</strong></p><p>W Davis, S J Van Rensburg, M J Kotze, L Fisher, M Jalali, F J Cronje, K Moremi, J Gamieldien, D Geiger, M Rensburg, R van Toorn, M J de Klerk, G M Hon, T Matsha, S Hassan, R T Erasmus</p><p><strong>69. Analysis of the COMT 472 G&gt;A (rs4680) polymorphism in relation to environmental influences as contributing factors in patients with schizophrenia</strong></p><p>D de Klerk, S J van Rensburg, R A Emsley, D Geiger, M Rensburg, R T Erasmus, M J Kotze</p><p><strong>70. Dietary folate intake, homocysteine levels and MTHFR mutation detection in South African patients with depression: Test development for clinical application </strong></p><p>D Delport, N vand der Merwe, R Schoeman, M J Kotze</p><p><strong>71. The use ofexome sequencing for antipsychotic pharmacogenomic applications in South African schizophrenia patients</strong></p><p>B Drogmoller, D Niehaus, G Wright, B Chiliza, L Asmal, R Emsley, L Warnich</p><p><strong>72. The effects of HIV on the ventral-striatal reward system</strong></p><p>S du Plessis, M Vink, J Joska, E Koutsilieri, C Scheller, B Spottiswoode, D Stein, R Emsley</p><p><strong>73. Xenomelia relates to asymmetrical insular activity: A case study of fMRI</strong></p><p>S du Plessis, M Vink, L Asmal</p><p><strong>74. Maternal mental helath: A prospective naturalistic study of the outcome of pregancy in women with major psychiatric disorders in an African country</strong></p><p>E du Toit, L Koen, D Niehaus, B Vythilingum, E Jordaan, J Leppanen</p><p><strong>75. Prefrontal cortical thinning and subcortical volume decrease in HIV-positive children with encephalopathy</strong></p><p>J P Fouche, B Spottiswoode, K Donald, D Stein, J Hoare</p><p><strong>76. H-magnetic resonance spectroscopy metabolites in schizophrenia</strong></p><p>F Howells, J Hsieh, H Temmingh, D J Stein</p><p><strong>77. Hypothesis for the development of persistent methamphetamine-induced psychosis</strong></p><p><strong></strong> J Hsieh, D J Stein, F M Howells</p><p><strong>78. Culture, religion, spirituality and psychiatric practice: The SASOP Spirituality and Psychiatry Special Interest Group Action Plan for 2012-2014</strong></p><p>B Janse van Rensburg</p><p><strong>79. Cocaine reduces the efficiency of dopamine uptake in a rodent model of attention-deficit/hyperactivity disorder: An <em>in vivo</em> electrochemical study</strong></p><p><strong></strong>L Kellaway, J S Womersley, D J Stein, G A Gerhardt, V A Russell</p><p><strong>80. Kleine-Levin syndrome: Case in an adolescent psychiatric unit</strong></p><p>A Lachman</p><p><strong>81. Increased inflammatory stress specific clinical, lifestyle and therapeutic variables in patients receiving treatment for stress, anxiety or depressive symptoms</strong></p><p>H Luckhoff, M Kotze, S Janse van Rensburg, D Geiger</p><p><strong>82. Catatonia: An eight-case series report</strong></p><p>M Mabenge, Z Zingela, S van Wyk</p><p><strong>83. Relationship between anxiety sensitivity and childhood trauma in a random sample of adolescents from secondary schools in Cape Town</strong></p><p>L Martin, M Viljoen, S Seedat</p><p><strong>84. 'Making ethics real'. An overview of an ethics course presented by Fraser Health Ethics Services, BC, Canada</strong></p><p>JJ McCallaghan</p><p><strong>85. Clozapine discontinuation rates in a public healthcare setting</strong></p><p>M Moolman, W Esterhuysen, R Joubert, J C Lamprecht, M S Lubbe</p><p><strong>86. Retrospective review of clozapine monitoring in a publica sector psychiatric hospital and associated clinics</strong></p><p>M Moolman, W Esterhuysen, R Joubert, J C Lamprecht, M S Lubbe</p><p><strong>87. Association of an iron-related TMPRSS6 genetic variant c.2007 C&gt;7 (rs855791) with functional iron deficiency and its effect on multiple sclerosis risk in the South African population</strong></p><p>K Moremi, S J van Rensburg, L R Fisher, W Davis, F J Cronje, M Jalali Sefid Dashti, J Gamieldien, D Geiger, M Rensburg, R van Toorn, M J de Klerk, G M Hon, T Matsha, S Hassan, R T Erasmus, M Kidd, M J Kotze</p><p><strong>88. Identifying molecular mechanisms of apormophine-induced addictive behaviours</strong></p><p>Z Ndlazi, W Daniels, M Mabandla</p><p><strong>89. Effects of lifestyle factors and biochemistry on the major neck blood vessels in patients with mutiple sclerosis</strong></p><p>M Nelson, S J van Rensburg, M J Kotze, F Isaacs, S Hassan</p><p><strong>90. Nicotine protects against dopamine neurodegenration and improves motor deficits in a Parkinsonian rat model</strong></p><p>N Ngema, P Ngema, M Mabandla, W Daniels</p><p><strong>91. Cognition: Probing anatomical substrates</strong></p><p>H Nowbath</p><p><strong>92. Chronic exposure to light reverses the effects of maternal separation on the rat prefrontal cortex</strong></p><p>V Russel, J Dimatelis</p><p><strong>93. Evaluating a new drug to combat Alzheimer's disease</strong></p><p>S Sibiya, W M U Daniels, M V Mabandla</p><p><strong>94. Structural brain changes in HIV-infected women with and without childhood trauma</strong></p><p>G Spies, F Ahmed, C Fennema-Notestine, S Archibald, S Seedat</p><p><strong>95. Nicotine-stimulated release of hippocampal norepinephrine is reduced in an animal model of attention-deficit/ hyperactivity disorder: the spontaneously hypertensive rat</strong></p><p>T Sterley</p><p><strong>96. Brain-derive neurotrophic factor (BDNF) protein levels in anxiety disorders: Systematic review and meta-regression analysis</strong></p><p>S Suliman, S M J Hemmings, S Seedat</p><p><strong>97. A 12-month retrospective audit of the demographic and clinical profile of mental healthcare users admitted to a district level hospital in the Western Cape, South Africa</strong></p><p>E Thomas, K J Cloete, M Kidd, H Lategan</p><p><strong>98. Magnesium recurarization: A comparison between reversal of neuromuscular block with sugammadex v. neostigmine/ glycopyrrolate in an <em>in vivo</em> rat model</strong></p><p><strong></strong>M van den Berg, M F M James, L A Kellaway</p><p><strong>99. Identification of breast cancer patients at increased risk of 'chemobrain': Case study and review of the literature</strong></p><p>N van der Merwe, R Pienaar, S J van Rensburg, J Bezuidenhout, M J Kotze</p><p><strong>100. The protective role of HAART and NAZA in HIV Tat protein-induced hippocampal cell death</strong></p><p>S Zulu, W M U Daniels, M V Mabandla</p>

Included in the Convention on the Rights of the Child, 1989 (UN Children's Convention) is the right of children to the highest attainable standard of health. In terms of article 4 of the UN Children's Convention, in implementing the UN Children's Convention state parties must "undertake all appropriate legislative, administrative, and other measures for the implementation of the rights recognised in the present Convention". South Africa showed its commitment to protecting and promoting children's health when it ratified the UN Children's Convention and subsequently adopted the Constitution of the Republic of South Africa, 1996, which includes provisions guaranteeing the health rights of children. South Africa also showed commitment to giving legislative effect to the protection and promotion of children's health by promulgating the National Health Act 61 of 2003, the Children's Act 38 of 2005 and the Mental Health Care Act 17 of 2002. The article evaluates existing policy and legislation affecting child health in order to assess how well South African legislation addresses the issue of children's healthcare rights and whether or not it complies with its international law and constitutional obligations in this regard. The article concludes that although much legislation exists, none provides comprehensively for children's healthcare rights, and there are many gaps in existing legislation. Most importantly, there is no reference to the core minimum requirements for the state in providing for the health of children, particularly in the way of healthcare services and nutrition. Further, there is a complete lack of legislation which protects the health needs of children with disabilities. In order to ensure that the health rights of children are protected and promoted, we propose more comprehensive legislative protection.

The research in this article deals with the effect of a teacher training course on vocabulary teaching and learning. Two months before, and three months after an intensive vocabulary teaching training course, four teachers of different grades in primary education were observed and interviewed, and their pupils' retention of the wTords taught was tested before and after the vocabulary lessons in question. The observations and interviews focused on six categories of vocabulary teaching: irord selection, preparation, sewantisation, consolidation, evaluation and registration (e.g., Nation, 2001). On a reference scale formulated in Van den Nulft & Verhallen (2005), the teachers scored 28% on 'basic level' before the training, and they turned out to score 68% on this 'basic level' scale three months after the training. The pupils appeared to profit from the progress their teachers made in vocabulary teaching. Their retention of the words they had to learn in these lessons rose from 8% learning improvement before the training, to 38% after the training. A learning improvement of 8% is considered very low in intentional learning situations (being comparable to incidental learning); a learning improvement of 30-50% is normal in intentional word learning situations. Given the average learning improvement of the pupils, and the quite low level of the teachers on the reference scale of vocabulary teaching, these teachers still need intensive and professional coaching to improve their vocabulary teaching.

The pandemic of COVID-19 has afflicted every individual and has initiated a cascade of directly or indirectly involved events in precipitating mental health issues. The human species is a wanderer and hunter-gatherer by nature, and physical social distancing and nationwide lockdown have confined an individual to physical isolation. The present review article was conceived to address psychosocial and other issues and their aetiology related to the current pandemic of COVID-19. The elderly age group has most suffered the wrath of SARS-CoV-2, and social isolation as a preventive measure may further induce mental health issues. Animal model studies have demonstrated an inappropriate interacting endogenous neurotransmitter milieu of dopamine, serotonin, glutamate, and opioids, induced by social isolation that could probably lead to observable phenomena of deviant psychosocial behavior. Conflicting and manipulated information related to COVID-19 on social media has also been recognized as a global threat. Psychological stress during the current pandemic in frontline health care workers, migrant workers, children, and adolescents is also a serious concern. Mental health issues in the current situation could also be induced by being quarantined, uncertainty in business, jobs, economy, hampered academic activities, increased screen time on social media, and domestic violence incidences. The gravity of mental health issues associated with the pandemic of COVID-19 should be identified at the earliest. Mental health organization dedicated to current and future pandemics should be established along with Government policies addressing psychological issues to prevent and treat mental health issues need to be developed. References World Health Organization (WHO) Coronavirus Disease (COVID-19) Dashboard. 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AbstractThis paper analyses the combinatorial restrictions that operate in clitic clusters in certain Eastern Iberian varieties (Aragonese, Spanish, and Catalan). In particular, I focus on the combination of third person clitics. As it is well known, in some Romance varieties the combination of a third person accusative clitic and a third person dative clitic is banned (the so-called ∗le lo restriction, Bonet, Eulàlia. 1991. Morphology after syntax: Pronominal clitics in Romance. Cambridge: Massachusetts Institute of Technology Dissertation; Cuervo, María Cristina. 2013. Spanish clitic clusters: Three of a perfect pair. Borealis: An International Journal of Hispanic Linguistics 2. 191–220; Nevins, Andrew. 2007. The representation of third person and its consequences for person-case effects. Natural Language & Linguistic Theory 25(2). 273–313; Ordóñez, Francisco. 2002. Some clitic combinations in the syntax of Romance. Catalan Journal of Linguistics 1. 201–224, Ordóñez, Francisco. 2012. Clitics in Spanish. In José I. Hualde, Antxon Olarrea & Erin O’Rouke (eds.), The handbook of Spanish Linguistics, 423–453. Malden: Wiley-Blackwell; Perlmutter, David. 1971. Deep and surface structure constraints in syntax. New York: Holt, Rinehart & Winston). In order to license this troublesome combination, languages resort to different ‘repair strategies’ modifying the structure of one of the merged clitics. In the literature on clitic combinations, there have been two main proposals of analysis: morphological and syntactical. In this paper, I put forward an analysis based on the Distinctness Condition (Hiraiwa, Ken. 2010. The syntactic OCP. In Yukio Otsu (ed.), The proceedings of the 11th Tokyo Conference on Psycholinguistics, 35–56. Hituzi: Tokyo; Neeleman, Ad & Hans van de Koot. 2005. Syntactic haplology. In Martin Everaert & Henk van Riemsdijk (eds.), The Blackwell companion to syntax, 685–710. Wiley-Blackwell; Perlmutter, David. 1971. Deep and surface structure constraints in syntax. New York: Holt, Rinehart & Winston; Richards, Norvin. 2010. Uttering trees, vol. 56. Cambridge, MA: MIT Press; Van Riemsdijk, Henk. 1998. Categorial feature magnetism: The endocentricity and distribution of projections. The Journal of Comparative Germanic Linguistics 2(1). 1–48; Yip, Moira. 1998. Identity avoxidance in phonology and morphology. In Steven G. Lapointe, Diane K. Brentari & Patrick M. Farell (eds.), Mophology and its relation to phonology and syntax, 216–246. Stanford, CA: CSLI). Specifically, I argue that the restrictions that constraint clitic combinations are due to the impossibility to linearize two identical syntactic objects, such as <XP, XP> (Chomsky, Noam. 2013. Problems of projection. Lingua 130. 33–49; Chomsky, Noam. 2015. Problems of projection. In Elisa Di Domenico, Cornelia Hamann & Simona Matteini (eds.), Structures, strategies and beyond: Studies in honour of Adriana Belletti, 1–16. Amsterdam: John Benjamins; Moro, Andrea. 2000. Dynamic antisymmetry (No. 38). Cambridge, MA: MIT Press; Richards, Norvin. 2010. Uttering trees, vol. 56. Cambridge, MA: MIT Press). From this perspective, cross-linguistic variation is the result of different ‘repair strategies’ languages deploy to make <XP, XP> objects linearizable (Richards, Norvin. 2010. Uttering trees, vol. 56. Cambridge, MA: MIT Press).

10063 Background: Gimatecan, a new oral camptothecin derivative, showed early clinical activity in patients with STS during the phase I clinical development. Methods: Patients with different histological types of STS, including rhabdomyosarcoma and Ewing's sarcoma of soft parts, and advanced or metastatic disease pretreated with anthracycline and ifosfamide were included. The principal inclusion criteria were: disease not curable with surgery and/or radiation, presence of a measurable progressive lesion(s), pretreatment with 1–3 lines of chemotherapy including adjuvant regimens, ECOG PS 0 to 1, age = 18 years. Gimatecan 0.8 mg/m2 was administered orally daily for five consecutive days every 4 weeks. Radiological response was assessed every two cycles by RECIST. A multicenter two stage Simon's optimal design was used to evaluate the single agent activity of gimatecan, also by means of a hierarchical Bayesian model to evaluate treatment effects within the histological subtypes, using the rate of progression free survival at 4 months (PFS- 4) (Van Glabbeke, Eur J Cancer 38: 543–549, 2002). Other objectives were safety, TTP, pharmacokinetics and biomarker evaluations. Results: From August 2005 to December 2006 40 patients, 24 (60%) males and 16 (40%) females, median age 48 years (range 21 - 77), were included in 5 European sites. As of December 2006, 14 patients (35%) achieved stable disease after a median of 4 (range 3–9) cycles, and the PFS-4 is 15% (hystotypes: leiomyosarcoma, liposarcoma, Ewing, synovial and unclassified); all PFS-4 patients had liver or pleuropulmonary metastases. A prolonged arrest of progression was observed in one Ewing (up to 9 months), in one leiomyosarcoma and one liposarcoma (up to 8 and 7 months respectively, both patients still on treatment). Main G3/4 toxicity was hematological, namely anemia (7.5%), thrombocytopenia (5%) and neutropenia (2.5%). Conclusions: These data suggest that oral gimatecan has produced durable disease stabilization and is well tolerated with less than 10% grade 3/4 haematological toxicities. [Table: see text]

Abstract Background and objective Fludarabine in combination with cyclophosphamide (FC) plus rituximab (R) is an effective treatment for newly diagnosed as well as relapsed follicular lymphoma (Tam 2004; Keating 2005; Sacchi 2007). Maintenance treatment with R, after different induction treatments, improves overall and progression-free survival (Forstpointner 2006; van Oers 2006). Therefore, we aimed to evaluate the efficacy and safety of the FC-R regime followed by maintenance doses of R. Patients and Methods We present an intermediate report of the one-arm study in which 75 previously untreated patients with a diagnosis of follicular non-Hodgkin’s lymphoma in Ann Arbor stage II–IV were included between October 2004–2006. Seventy four were assessed for safety after receiving at least one FC-R dose (F: 3x25 mg/m2 and C: 1 g/m2; R: 375mg/m2), and 72 for response to treatment. Patients aged 53.4 years in average, one in five showed bulky disease and 72.2% Ann Arbor IV staging. FLIPI index determined 23.9% patients with low (0–1) score, 38% with intermediate (2) and 38% with poor score (3). A total of 47 patients presented some molecular alteration in PB or BM. Results Induction therapy was delivered throughout 4–6 courses, resulting in 91% complete responses (CR) and 9% partial responses (PR) (Table 1). From the patients who presented monoclonal population at diagnosis, 40 were evaluated for molecular response after induction and only 1 remained MDR positive for bcl2/IgH. Overall survival (OS) at 24 months was 87.5%, and two patients presented progressive disease within this period. The median OS has not been reached at this evaluation. To the date, 262 adverse effects grade 3–4 (32.6%) have been documented (80.9% neutropenias) and 80 infectious complications were recorded (23.8% grade 3–4). Three patients died from respiratory diseases, two from acute leukemia, and six from other causes. Table 1 EVOLUTION OF RESPONSE Evaluated Response at End of Induction Therapy Evaluated Response Post-Course 3 Assesable End Ind. (n=67) Missing End Ind. (n=5) CR: complete response; uCR: unconfirmed CR; PR: partial response; NE: not evaluated; WD: withdrawn; EX: exitus. Assesable PC3 (n=70) CRITERIA CR PR NE WD EX 14 CR 12 - 1 1 - 32 uCR 31 - 1 - - 24 PR 16 6 - - 2 2 Missing PC3 (NE) 2 - - - - 72 Total 61 6 2 1 2 Conclusions The FC-R has proven a potent antitumoral activity in untreated follicular lymphoma patients, rendering very high clinical and molecular responses. However, as reported in similar studies (Hochster 2007 ASCO), the high incidence of prolonged neutropenias and lymphopenias developed as consequence of the chemotherapy regime, questions the safety of the induction treatment.

ABSTRACTLenses in laparoscopes, arthroscopes, and laryngoscopes fog during closed body surgery due to humidity from bodily fluids and differences between body and operating room temperatures.1,2 Surgeons must repeatedly remove, clean, and reinsert scopes that are obscured by fog. As a result, surgery duration, infection risks, and scarring from air exposure increase.3,4 Current methods to address fogging introduce other complications. Acidic alcohol-based coatings scar tissue and quickly evaporate, and heated lenses require reheating every 5 to 20 minutes.3,4 This paper presents a new super-hydrophilic, biocompatible, non-toxic, pH neutral (7.2-7.4), and long-lasting anti-fog coating called VitreOx™.5-7 VitreOx™ can be used wet or dry, without use of alcohol, heat, or fluid evacuation. When applied as a liquid, it easily espouses lenses’ surfaces and edges, and dries within seconds to form a permanently super-hydrophilic surface on silica and polymer surfaces. VitreOx™ avoids current shortfalls by eliminating frequent reapplications, avoiding reapplication for surgeries lasting up to 72 hours.VitreOx™'s anti-fog properties can be explained by nucleation and growth theory for thin films condensation: 1) 3-D droplets, resulting in fogging; 2) 2-D sheets resulting in a flat transparent film; or 3) mixed 3-D on 2-D, resulting in optical distortion. On hydrophobic surfaces (e.g. lenses), condensation occurs with fogging via spherical 3-D droplets, as in the Volmer-Weber model. 3-D droplets scatter light in all directions through refraction yielding opaque or translucent films (fog). VitreOx™ applied to hydrophobic lenses renders them super-hydrophilic. Similar to the 2-D Frank Van-der-Merwe Growth Mode, condensation with uniform wetting yields transparent 2-D films that do not distort optical images transmission.In vitro and in vivo studies of VitreOx™ were conducted to measure performance and duration of anti-fog effectiveness and bio-compatibility. In vitro tests spanned from 3 to 72 hours over a 3-year range. Side-by-side in vivo gastro-endoscopies were conducted on Yucatan™ swine for 90 minutes using 1) VitreOx™, 2) bare lens, and 3) Covidien Clearify™ surfactant with warmer. VitreOx™ coated lenses did not fog nor need reapplication for 90 minutes, while Covidien Clearify™ lasted 38 minutes without fogging, requiring retreatment. No adverse reaction was observed on swines exposed toVitreOx™, in surgery and 12 months thereafter.

3560 Background: Antiangiogenic agents have recently demonstrated promising activity in patients with advanced RCC. AZD2171 is an oral, highly potent and selective inhibitor of VEGFR-1, -2 and -3 tyrosine kinases, and is currently in Phase II/III development in NSCLC and CRC. Safety and efficacy results are presented for patients with RCC who participated in two Phase I dose-escalation studies of AZD2171 in advanced solid tumors. Methods: Patients with advanced recurrent metastatic RCC received once-daily oral AZD2171, either as monotherapy (Drevs et al. Proc Am Soc Clin Oncol 2005: abst 3002; completed) or in combination with gefitinib (van Cruijsen et al. Proc Am Soc Clin Oncol 2006: abst 3017; ongoing). All patients had received prior interferon-a ± interleukin-2/ 5-FU or radiotherapy. Results: Twenty-one patients received AZD2171, 4 as monotherapy (20 mg, n=1; 60 mg, n=3) and 17 as combination therapy (AZD2171 20–45 mg with gefitinib 250 or 500 mg). The adverse event (AE) profile in RCC patients was similar to that reported previously for all patients in these studies; hypertension, dysphonia, nausea, vomiting, diarrhea and fatigue were the most common AEs. The most common CTC grade 3 AEs were hypertension (n=5) and diarrhea (n=4). Reductions in plasma levels of sVEGFR-2 were observed in RCC patients when AZD2171 was administered as a monotherapy or in combination with gefitinib; the reductions in the combination study were dose- and time-dependent (sustained). Nineteen patients were evaluable for efficacy (RECIST): 2/3 monotherapy patients experienced tumor regression, one of which was confirmed as a partial response (duration of response [DR] 87 days); in the combination study, 6/16 (38%) patients achieved a partial response (median DR 6 months; 3/6 ongoing at data cut off) and 7/16 (44%) patients had stable disease (4/7 had a confirmed 10–30% reduction in maximum tumor diameter). Conclusion: AZD2171, both as monotherapy and in combination with gefitinib, showed encouraging antitumor activity and a manageable toxicity profile in patients with advanced RCC who had failed prior therapy. A randomized Phase II investigation of AZD2171 monotherapy (45 mg/day) vs placebo in patients with advanced RCC is ongoing. [Table: see text]

Abstract Abstract 40 Introduction: This independent trial was designed to evaluate the efficacy of bortezomib (B) during induction and maintenance on progression-free survival (PFS) in patients with newly diagnosed symptomatic MM, who were candidates for high-dose therapy. Patients were randomly assigned to 3 cycles of standard VAD (arm A) or PAD (Arm B); PAD was dosed as B 1.3 mg/m2, days 1,4,8,11, doxorubicin 9 mg/m2, days 1–4, dexamethasone 40 mg, days 1–4, 9–12, 17–20). Patients received one (HOVON) or two (GMMG) high-dose melphalan (HDM) 200 mg/m2 with ASCT. Maintenance consisted of thalidomide (T) 50 mg daily (arm A) or B 1.3 mg/m2, 2-weekly (arm B) for 2 years. Primary endpoint was PFS, other endpoints were complete response (CR) (EBMT), immunofixation positive CR (nCR), VGPR pre-and post HDM and survival (OS). The protocol specified analysis was intention-to-treat and censored for patients who received allo-SCT after HDM1 (n=46). We report the analysis of the first 626 randomized patients. The final analysis of all patients will be presented at the meeting. Results: 13 patients were excluded (7 not eligible, 6 not evaluable). The two arms (A:n=305;B:n=308) were well balanced for age, Salmon/Durie stage II/III, renal failure (11%), and serum B2M. Medium follow-up is 40 months. 89% of patients completed induction and HDM1. In GMMG after HDM1 80% of patients received 2nd HDM. Full dose B could be administered in 82% of patients. Polyneuropathy (PNP) WHO gr 3+4 occurred in 7% (arm A) and 16% (arm B). 204 (67%, arm A) and 174 (57%, arm B) patients started maintenance. 64% of patients tolerated full dose B and 27% reduced dose. 47% of patients on B maintenance went off protocol because of toxicity (9%), progression (29%) or other (9%). In contrast 64 % on T maintenance went off protocol because of toxicity (31%), progression (31%) or other (2%). nCR/CR rates were 7/9% (arm A) vs 9/21% (arm B) at 3 months after HDM-1 and 12/26% (arm A) vs 12/38% (arm B) on protocol. ≥VGPR in arm-A vs arm-B were 40% vs 60% after HDM-1 and 61% vs 75% on protocol. PFS was superior in arm B (HR 0.81, p=0.047; adjusted for ISS: HR 0.81, p=0.056). PFS at 36 months was 42% (arm A) vs 46% (arm B). Multivariate Cox regression showed treatment arm (p=0.037), IgA (p=0.007), ISS stage (p=0.007), WHO Performance Status (p<0.0001), del13/13q- (p=0.015) and study group (2nd HDM) (p=0.015) as significant PFS variables. Patients treated with bortezomib had a better OS (HR 0.74, p=0.048), with study arm, WHO, IgA, ISS stage and del13/13q- as significant variables. Subgroup analysis of response at 12 months showed no impact on PFS and an impact of VGPR/nCR/CR on OS only in arm A. Adverse cytogenetic markers (p<0.05) in the combined group were 13q14, 17p-, t(4;14) for PFS and OS. Detailed FISH data are reported separately. The response and survival data of the subgroup analysis are given below. We conclude that B achieves high nCR/CR during induction, that B maintenance is well tolerated and is associated with additional responses. Bortezomib achieves superior PFS and results in an improvement of survival. This trial (EudraCT no. 2004-000944-26) was supported by the Dutch Cancer Foundation, the German Federal Ministry of Education and Research and a grant from Janssen-Cilag. Disclosures: Sonneveld: celgene: Membership on an entity's Board of Directors or advisory committees; janssen-Cilag: Membership on an entity's Board of Directors or advisory committees; millennium: Membership on an entity's Board of Directors or advisory committees. Off Label Use: bortezomib, induction treatment prior to high dose therapy. Schmidt-Wolf:celgene: Membership on an entity's Board of Directors or advisory committees; janssen-Cilag: Research Funding. van de Velde:Johnson & Johnson: Employment, Equity Ownership. Delforge:celgene: Membership on an entity's Board of Directors or advisory committees; janssen-cilag: Membership on an entity's Board of Directors or advisory committees. Weisel:orthobiotech: Consultancy, Membership on an entity's Board of Directors or advisory committees; celgene: Membership on an entity's Board of Directors or advisory committees. Scheid:orthobiotech: Honoraria. Goldschmidt:celgene: Honoraria, Research Funding; amgen: Honoraria, Research Funding; novartis: Honoraria, Research Funding; orthobiotech: Honoraria, Research Funding; roche: Honoraria, Research Funding.

In this paper, seismic activity characteristics in the East Sea area was analyzed by authors on the base of the unified earthquake catalog (1900-2017), including 131505 events with magnitude 3 ≤ Mw ≤ 8.4. The seismic intensity in the East Sea during the period 1900-2017 is characterized by the earthquake representative level Mw = 4.7. The strong earthquake activity in the East Sea area clearly shows the regularity in each stage. In the period from 1900 to 2017, the East Sea area has four periods of strong earthquake activity, each stage is nearly 30 years with particular characteristics. The distribution of the maximum earthquake quantities by years has a cyclicity in all four periods. In each stage there are 1-2 strong earthquakes with Mmax ≥ 8.0. The strong earthquakes with Mmax ≥ 7.5 have occurred by a repeatable rule of 3-5 years in all four stages. This allows the prediction of the maximum earthquake repeat cycle of Mmax ≥ 7.5 in the study area is 3-5 years. In other hand, the maximum magnitude values for the East Sea region has assessed by GEV method with several different predict periods (20, 40, 60, 80, 100 years), with predicted probability 80%. We concluded that it is possible that earthquake have Mmax = 8.7 will occur in next 100 years.ReferencesBautista C.B., Bautista M.L.P., Oike K., Wu F.T., Punongbayan R.S., 2001. A new insight on the geometry of subducting slabs in northern Luzon, Philippines. Tectonophysics, 339, 279-310.Bui Cong Que, et al., 2010. Seismic and tsunamis hazard in coastal Viet Nam. Natural Science and Technology Publishing House, 311p.Bui Van Duan, Nguyen Cong Thang, Nguyen Van Vuong, Pham Dinh Nguyen, 2013. The magnitude of the largest possible earthquake in the Muong La-Bac Yen fault zone. J. Sci. of the Earth, 35, 53-59 (in Vietnamese).Cao Dinh Trieu, Pham Nam Hung, 2008. Deep-seated fault zone presents the risk of strong earthquakes in the East and South Vietnam Sea. 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New approach to the Characterization of Mmax and of the Tail of the Distribution of Earthquake Magnitudes. Pure and Applied Geophysics, 165, 847-888.Pisarenko V.F, Sornette D. and Rodkin M.V., 2010. Distribution of maximum Earthquake magnitudes in future time intervals: application to the seismicity of Japan (1923-2007). EPS (Earth, Planets and Space), 62, 567-578.Pisarenko V.F., Rodkin M.V, and Rukavishnikova T.A., 2014. Estimation of the Probability of Strongest Seismic Disasters Based on the Extreme Value Theory. Physics of the Solid Earth, 50(3), 311-324.Pisarenko V.F., Rodkin M.V. and et al., 2012. New general quantile approach to the seismic rick assessment application to the Vietnam region. //Proceedings of the International Conference on "Geophysics - Cooperation and Sustainable Development." Science and Technology Publishing House. Hanoi, 161-167.Vu Thanh Ca, 2008. Report on the project to build a map of tsunami warning for coastal areas of Vietnam. 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Abstract Abstract 5168 Chelation treatment of iron overload from chronic blood (RBC) transfusion is still a challenge to both, patients and medical caretakers. Different treatment regimes have been recommended so far, especially for chronically transfused patients with low or even normal liver iron concentration. We report the results from 16 regularly transfused patients with thalassemia major (TM) who were on iron chelation treatment under normal to mild liver iron concentration (LIC). All patients received deferoxamine (DFO) treatment before they changed to deferasirox (DSX) treatment. 16 TM patients (mean age 13.6 y) were treated with DSX (median dose 18 mg/kg/d, range: 7 – 33 mg/kg/d) for 6 to 71 months. Liver iron measurements by biomagnetic susceptometry (BLS) and/or MRI-R2 as well as cardiac MRI-R2* were performed in intervals of 6 to 12 months. The median LIC was 782 μ g/g-liver wet weight (range: 460 μ g – 1122 μ g). Median RBC transfusion rate was 8500 ml/y, equivalent to about 2 erythrocyte concentrates per 3 weeks or a daily iron influx of 16.2 mg/d. For each measurement interval, the ratio of daily iron influx and DSX dose rate was calculated. This represents the equilibrium molar efficacy for iron balance. In all 16 TM patients no severe side effects were observed and creatinine was in the normal range of < 0.9 mg/d throughout the treatment with DSX. From baseline DFO treatment interval to the endpoint of DSX treatment, liver iron decreased by 124 – 4689 μ g/g-liver (conversion factor of 6 for mg/g-dry-wgt), while serum ferritin decreased by -596 to 8283 μ g/l. For all measurement intervals, molar chelation efficacies between 18 % and 56 % were calculated at equilibrium with a median efficacy of 31 % (interquartile range = 16 %). This agrees with molar efficacies of DSX reported earlier, but for relatively higher LIC and chelation doses (Blood 2005; 106(11):#2690 and Blood 2007; 110(11):#2776). The cardiac R2* (median R2* = 38 s-1) was either below the normal threshold of 50 s-1 (T2* > 20 ms) or decreased by about 24 %/y under DSX treatment. In these few patients at low LIC, this was even higher than recently reported. Conclusion: Even in patients with normal to mild LIC iron chelation treatment with DSX is safe, does not result in increased creatinine levels or severe side effects and is as efficient as in patients with higher LIC. Disclosures: No relevant conflicts of interest to declare.

In the eastern United States, Monilinia laxa (Aderh. & Ruhl.) Honey has only been reported on tart cherry in New York (NY) (1). As a result of considerable rain in May of 2009 and 2011, an ornamental planting of Kwanzan cherries in Middletown, Rhode Island (RI), a planting of sweet cherry cvs. Ulster, Hedelfingen, Sam, and Lapins in Lanesboro, Massachusetts (MA), and plantings of apricot cvs. Harcot and Hargrande in Albion, Aurora, and Geneva, NY, and Harogem in Lanesboro, MA developed severe shoot blight (>15 to 100% of first-year shoots). Blighted shoots were wilted with the blight encompassing the distal end and often extending into second-year tissue with a distinct sunken margin. Leaves on symptomatic shoots had flushed, but were blighted and light brown. Blossom spurs were often blighted and gummosis was frequently observed at the base. In these same years, sweet cherry cv. Black Gold in Walworth, NY and plum cv. Stanley in Olcott, NY developed severe fruit rot (35 to 70% incidence). Plantings suffering from fruit rot had fruit lesions that began as pale brown, soft lesions with indiscriminant margins that covered 15 to 85% of the fruit surface area. Many blighted spurs, shoot tissues, and infected fruit were sporulating with tan-to-buff colored conidia produced in chains. From each planting with shoot blight, shoot tips were removed for pathogen isolation. Sections of symptomatic shoots (5 cm long) were surface sterilized in 0.6% NaOCl for 1 min and rinsed in sterile dH20. From plantings displaying blighted spurs or fruit rot, isolation was attempted directly from sporulating tissue. Cross sections of sterilized shoot tissue (3 mm thick) or tufts of sporulation from fruit and spurs were placed on potato dextrose agar amended with 50 μg/ml of streptomycin sulfate. After incubation at 24°C for 5 days, colonies with lobed margins, commonly described for M. laxa (4), were obtained. Several colonies resembling M. fructicola were isolated from all locations, but the majority of isolates from spurs and shoots resembled M. laxa. Conidia from both colony morphotypes were lemon shaped, but as expected, those from putative M. laxa isolates were smaller (10.75 × 12.0 μm) compared with those from putative M. fructicola isolates (15.75 × 18.25 μm) (4). Confirmation of M. laxa was further achieved by PCR amplification of the β-tubulin gene using M. laxa-specific primers as previously described (3). Pathogenicity of M. laxa isolates was proven by inoculating fruit of the stone fruit crop from which they were isolated as previously described (2). Fruit inoculated with M. laxa developed brown, soft sporulating lesions identical to the original observations, while those inoculated with water remained healthy. M. laxa was reisolated from symptomatic shoots and spurs, but not from water-inoculated tissues. The presence of M. laxa has been reported on tart cherries in NY (1), but to our knowledge, this is the first instance of economically devastating shoot blight on apricot in NY and MA, ornamental cherry in RI, and sweet cherry in MA and fruit rot on sweet cherry and plum in NY caused by M. laxa. In wet seasons, stone fruit growers may need to revise their chemical management programs to better prepare for M. laxa epidemics on several stone fruit species. References: (1) K. D. Cox and S. M. Villani. Plant Dis. 94:783, 2010. (2) K. D. Cox and S. M. Villani. Plant Dis. 95:828, 2011. (3) Z. Ma et al. Pest Manag. Sci. 61:449, 2005. J.M. (4) G. C. M. van Leeuwen and H. A. van Kesteren. Can. J. Bot. 76:2042, 1998.

Abstract Pediatric acute myeloid leukemia (AML) is a heterogeneous disease and 30-40% of the patients still die. Prognosis is dependent on relevant genetic aberrations. Although many driving genetic alterations causing AML have been defined, in ~20% of the pediatric AML patients the oncogenic events remain unidentified. The ETS-Variant gene 6 (ETV6) encodes a transcription factor that functions as a tumor suppressor gene and is required for proper hematopoiesis in the bone marrow niche. Point mutations, deletions and translocations can lead to silencing of the gene, resulting in loss of transcriptional repression activity. ETV6 aberrations strongly associate with leukemia. In pediatric B-cell precursor acute lymphoblastic leukemia, translocation ETV6/RUNX1 occurs in ~25% of cases. Mutations in ETV6 are identified in ~25% of early immature T-cell ALL and also reported as event in adult AML (Van Vlierberghe et al, J Exp Med 2011; Barjesteh van Waalwijk van Doorn-Khosrovani et al, Oncogene 2005). We previously reported that pediatric AML patients can be divided in three clusters based on HOX-expression; (1) low HOXA/B expression, (2) high HOXA and low HOXB expression, and (3) high HOXA/B expression, and identified new repetitive genetic abnormalities in the third cluster, especially in NUP98. Cluster 1 is mainly represented by core-binding factor (CBF) AML, but in ~20% of these cases we did not find specific genetic abnormalities. Helton et al presented ETV6 aberrations in pediatric CBF-AML at ASH 2011, identified with whole genome sequencing, and with poor clinical outcome. We hypothesized that ETV6 aberrations might reduce the number of patients without known driving abnormality, especially in the low HOXA/B cluster. We screened a large representative de novo pediatric AML cohort for ETV6 mutations in exons 2-8 with direct sequencing, for ETV6 deletions by multiplex ligation-dependent probe amplification and for ETV6 translocations using split signal FISH, and analyzed outcome. In a well-characterized de novo pediatric AML cases with available gene-expression data, 6/275 (2.2%) patients had mutations affecting the predicted amino acid sequence of ETV6 and one had a silent mutation, 4/259 (1.5%) had an ETV6 deletion and 6/65 (9.2%) patients an MNX1/ETV6 translocation. Additionally, we identified 3 cases with a positive split signal FISH suggestive of a break in which ETV6 is involved, and a similar gene expression profile was found in these three cases. The aberrations of ETV6 were seen in patients of all three HOX-groups; n=9, n=6 and n=4 for cluster 1, 2 and 3 respectively. In patients with an ETV6 mutation (n=6) or deletion (n=4) 13 and 38 genes, respectively, were significantly up-regulated, including CLDN5,DPEP1 and BIRC7. This is consistent with the up-regulated genes in functional studies silencing ETV6 in LOUCY cells (Van Vlierberghe et al, J Exp Med 2011). High expression of BIRC7 has been associated with poor prognosis in adult acute leukemia (El-Mesallamy et al, Leuk Res 2011). The median age of patients with an ETV6-mutation or deletion (n=10) was 11.3 years (range 4.0-15.3) and 40% were female. Median WBC was significantly lower (15.1x109/L vs 47.0x109/L, p<0.01) in comparison to other pediatric AML cases. Other cytogenetic aberrations found in the ETV6-mutated or deleted cases were RUNX1/RUNX1T1 (n=3), PML/RARA (n=1), MLL/AF6 (n=1) and one case with an NPM1-mutation. Six out of ten patients encountered a relapse and one patient died of treatment-related mortality. The median age for patients with an ETV6-split signal FISH (n=9) was significantly lower compared to other pediatric AML cases (median 1.1 years vs 9.8, p<0.01), median WBC and sex did not differ. Five out of 9 encountered relapsed/refractory disease whereas 2 cases died of treatment-related mortality. The 3-yr pOS for all ETV6-aberrated patients taken together (n=19) was 37±11% vs 65±3% for the other pediatric AML patients (n=242, p<0.01); the 3-yr pEFS was 26±10% vs 46±3% (p=0.07), and 3-yr pCIR 47±13% vs 37±3% (p=0.24). We conclude that ETV6 aberrations are rare but recurrent in pediatric AML. ETV6 aberrations predict a poor survival, although there was no evidence for an increased relapse incidence in this small cohort. Disclosures No relevant conflicts of interest to declare.

Abstract Abstract 1800 Poster Board I-826 Background The prevalence of peripheral neuropathy (PNP) during the treatment of MM with Bortezomib is high. About 20% of patients develop a grade 3-4 PNP due to this treatment, and as a result Bortezomib treatment is stopped or a reduced dose is given. Therefore, there is a strong need to find markers which predict the susceptibility of a patient to develop Bortezomib related PNP. Materials and methods: Bortezomib treated patients from the Dutch/German Hovon 65 GMMG-HD4 trial and the French IFM-2005/01 trial were used for this analysis. In both trials, the efficacy of Bortezomib as induction treatment prior to high-dose therapy is evaluated and PNP status was recorded. Samples were genotyped using a custom-built molecular inversion probe (MIP)-based single nucleotide polymorphism (SNP) chip containing 3404 SNPs (Bank on a Cure program; Van Ness et al., 2008). In total, 232 patients who did not develop PNP were compared to 210 PNP cases (grade 1, n=82; grade 2 n=86, grade 3, n=31, grade 4, n=11). Results The data were processed on the basis of the following criteria. First, SNPs genotyped in less than 75% of the samples were removed (n=155). This resulted in elimination of 59% of the data with unknown genotype while only 1% of the genotyped data were lost. The remaining 41% of the missing data were imputed using BIMBAM (Guan et al., PLoS Genet. 4:e1000279, 2008). As reference panels, the data sets of the BOAC chips from this study, 500 random samples from the Rotterdam ERGO study (Köttgen et al., Nat. Genet. 41, 712–717, 2009) and 60 phased CEU HAPMAP samples were used. Secondly, SNPs were excluded which did not show any genotype variance and which were not in Hardy Weinberg equilibrium. As a last step the data was adjusted for stratification using Eigenstrat (Price et al., Nat. Genet. 38: 904–909, 2006). By removing 21 SNPs and 14 samples the variance between the IFM and Hovon was reduced to an acceptable level (p = 0.011). The resulting combined IFM/Hovon dataset now contained 2764 SNP and 428 samples. The data set was divided in 6/7 (n=367) part as a learning set and 1/7 (n=61) as a validation set. Possibly informative SNPs were selected using information gain as a feature selection method (Cover et al., Elements of information theory. New York, John Wiley, 1991). 66 SNPs with an information gain in allele and genotype frequency were selected (p value < 0.05 after permutation test (n=10000)). Classifiers generated by Partial C4.5 decision tree (PART), support vector machine (SVM) and Random forest learned on this set reached a better than random performance. Sensitivity, specificity, positive predictive value and negative predictive value were respectively 55%, 70%, 60%, and 66% for the PART classifier. Conclusion Preliminary classifiers generated by this dataset suggest that building a classifier with clinically relevant performance may be within reach. To this end, we will report on the outcome of different combinations of existing classifier methods and feature selection methods. Van Ness, B, Ramos, C, Haznadar, M, Hoering, A,Haessler, J, Crowley, J, Jacobus, S, Oken, M, Rajkumar, V, Greipp, P, Barlogie, B, Durie, B, Katz, M, Atluri, G, Ganf, G, Gupta, R, Steinbach, M, Kumar, V, Mushlin, R, Johnson, D, and Morgan, G. (2008). Genomic Variation in Myeloma: Design, content, and initial application of the Bank On A Cure SNP Panel to analysis of survival. BMC Medicine. 6:26. Disclosures Hanifi-Moghaddam: Skyline Diagnostics: Employment.

Pineapple lily (Eucomis vandermerwei) is grown for its exotic-looking flower spike that looks like a small pineapple. It is used as an accent plant in the garden or for its long-lasting cut flowers. In late November 2007, plant foliage was submitted to the California State Diagnostic Laboratory by a commercial grower for phytosanitary inspection before shipment of bulbs to Europe. Leaves had wet, brown, elliptical spots of 1 to 2 cm long, some with yellow halos. The interior of the older spots was pale brown and papery. Conidiophores typical of Botrytis spp. were observed on the abaxial side of the leaf in the interior of the older spots. Isolations on half-strength acidified potato dextrose agar (APDA) yielded a grayish mycelium with conidiophores developing on the plant tissue only. The agar was grayish yellow, especially when viewed from the underside. Conidiophores measured 275 to 650 μm × 15 to 20 μm (411.2 × 15.5 μm average). Conidia were light brown, subglobose to broadly ellipsoidal, and measuring 10 to 15 × 13 to 20 μm (11.8 × 16.2 μm average). Scattered, black sclerotia measuring 335 to 1,007 × 518 to 1,079 μm (668.9 × 743.9 μm average) formed on the medium after approximately 7 days. Pathogenicity was confirmed by inoculating four Eucomis spp. plants with one inoculation per leaf, four leaves per plant. Each leaf was wounded with a sterile pushpin and three agar plugs from 4-day-old cultures were placed in a plastic screw-cap lid filled with sterile water and clipped onto each wound. Plants were misted with water, covered with a plastic bag, and placed in a growth chamber at 16°C (12-h photoperiod) for 48 h after which the agar plugs and caps were removed. An equal number of plants were wounded and mock inoculated with APDA. Pathogenicity experiments were repeated. After 14 days, all inoculated leaves had lesions and the fungus was reisolated. No Botrytis spp. were isolated from the mock-inoculated plants. Our sequence of the intergenic spacer regions of one isolate, GenBank FJ10809, matched Botrytis hyacinthi sequence AJ716297 with 99.8% identity. Using molecular (3) and morphological characters (1,4), the pathogen was identified as B. hyacinthi, the cause of fire disease in hyacinths. This pathogen was described previously on hyacinths in Washington State (2), the Netherlands (4), and the United Kingdom (2). The importance and economic impact of this disease appears to be limited because it has only been observed on mature or senescing foliage and not bulbs. To our knowledge, this is the first report of B. hyacinthi causing a leaf spot on pineapple lily. References: (1) C. J. Gould et al. Plant Dis. Rep. 42:534, 1958. (2) W. C. Moore. Page 14 in: Diseases of Bulbs. Bull. No. 117. Minist. Agric. Fish. London, 1939. (3) M. Staats et al. Mol. Biol. Evol. 22:333, 2005. (4) J. Westerdijk and F. H. Van Beyma Thoe Kingma. Meded. Phytopathol. Lab. “Willie Commelin Scholten” 12:15, 1928.

El objetivo de este trabajo es evaluar un proyecto fotovoltaico como fuente de energía alternativa en el sector de salud primaria como estudio de caso, desde la perspectiva económica y social. La evaluación se basó en variables técnicas y económicas bajo los criterios de Valor Actual Neto (VAN) y Tasa interna de retorno (TIR), valorizando las reducciones de carbono (CO2) y utilizando la tasa de descuento social del Ministerio de Desarrollo Social. Los resultados son favorables y sugieren la ejecución de este proyecto como iniciativa de política pública. Sin embargo, queda en evidencia que en periodos de invierno no se cubre las necesidades energéticas, haciendo imprescindible diversificar la matriz con fuentes tradicionales. Palabras Clave: Energía solar fotovoltaica, sector salud, sustentabilidad, evaluación social. Referencias [1]Fondo Nacional de Salud (FONASA), Boletin Estadístico 2016-2017. Disponible: https://www.fonasa.cl/sites/fonasa/adjuntos/Boletin_Estadistico_2016_2017_2018. [2]Cisterna L, Améstica-Rivas L, Piderit M. Proyectos fotovoltaicos en generación distribuida ¿Rentabilidad privada o sustentabilidad ambiental?. Revista Politécnica. 2020; 45(2): en prensa. Disponible: https://revistapolitecnica.epn.edu.ec/ojs2/index.php/revista_politecnica2/issue/view/39. [3]Medina J. La dieta de dióxido de carbono CO2. Conciencia Tecnológica. 2010; 39: 50-53. Disponible: https://www.redalyc.org/articulo.oa?id=94415753009. [4]Mardones C. Muñoz, T. Impuesto al CO2 en el sector eléctrico chileno: efectividad y efectos macroeconómicos. Economía Chilena. 2017; 20(1): 4-25. Disponible: https://www.bcentral.cl/web/guest/articulos-publicados. [5]Ministerio del Medio Ambiente, Tercer Informe de Actualización Bienal de Chile, 2018. Disponible: https://mma.gob.cl/wp-content/uploads/2019/07/2018_NIR_CL.pdf. [6]Gallego Y, Arias R, Casas L, Sosa R. Análisis de la implementación de un parque fotovoltaico en la Universidad Central de las Villas. Ingeniería Energética, 2018; 39(2): 82-90. Disponible: http://rie.cujae.edu.cu/index.php/RIE/article/view/531. [7]Arias R, Pérez I. Nueva metodología para determinar los parámetros de un módulo fotovoltaico. Ingeniería Energética. 2018; 39(1): 38-47. Disponible: http://rie.cujae.edu.cu/index.php/RIE/article/view/557. [8]Plá J, Bolzi C, Durán J.C. Energía Solar Fotovoltaica. Generación Distribuida conectada a la red. Ciencia e Investigación. 2018; 68(1), 51-64. Disponible: http://aargentinapciencias.org/wp-content/uploads/2018/03/tomo68-1/4-Duran-cei68-1-5.pdf. [9]Hou G, Sun H, Jiang Z, Pan Z, Wang Y, Zhang X, Zhao Y, Yao Q. Life cycle assessment of grid-connected photovoltaic power generation from crystalline silicon solar modules in China. Applied Energy. 2016; 164 (15): 882-890. Disponible: https://doi.org/10.1016/j.apenergy.2015.11.023. [10]Baharwani V, Meena N, Dubey A, Brighu U, Mathur J. Life Cycle Analysis of Solar PV System: A Review. International Journal of Environmental Research and Development. 2014; 4(2): 183-190. Disponible: https://www.ripublication.com/ijerd_spl/ijerdv4n2spl_14.pdf [11]Rojas-Hernández I, Lizana F. Tiempo de recuperación de la energía para sistemas fotovoltaicos basados en silicio cristalino en Costa Rica. Ingeniería Energética. 2018; 39 (3):195-202. Disponible: http://rie.cujae.edu.cu/index.php/RIE/article/view/544. [12]World Economic Forum. Informe Energía. 2017. Disponible: https://es.weforum.org/agenda. [13]Zou L, Wang L, Lin A, Zhu H., Peng Y, Zhao Z. Estimation of global solar radiation using an artificial neural network based on an interpolation technique in southeast China. Journal of Atmospheric and Solar-Terrestrial Physics. 2016; 146: 110-122 Disponible: https://doi.org/10.1016/j.jastp.2016.05.013. [14]Crawley D, Lawrie, L, Winkelmann F, Buhl W, Huang C, Pedersend C, Strand R, Liesen R, Fisher D, Witte M, Glazer J. EnergyPlus: creating a new-generation building energy simulation program. Energy and Buildings. 2001; 33(4): 319-331.Disponible: https://doi.org/10.1016/S0378-7788(00)00114-6. [15]Larrain S, Stevens C, Paz M. Las fuentes renovables de energía y el uso eficiente. 2002. LOM Ediciones, Chile Disponible: http://www.archivochile.com/Chile_actual/patag_sin_repre/03/chact_hidroay-3%2000010.pdf. [16]World Economic Forum. Cuatro países que lideran las tendencias de energía solar en América Latina y el Caribe, 2017.Disponible: https://es.weforum.org/agenda/2017/05/cuatro-paises-que-lideran-las-tendencias-de-energia-solar-en-america-latina-y-el-caribe/. [17]Ministerio de Energía. Ley 20.571, Regula el pago de las tarifas eléctricas de las generadoras residenciales. 2012. Disponible: https://www.leychile.cl/Navegar?idNorma=1038211. [18]Comisón Nacional de Energía (CNE) de Chile. Reporte mensual sector energético. 2019; 50. Disponible: https://www.cne.cl. [19]Ministerio de Energía, Programa de Techos Solares Públicos, Reporte de costos. 2018. Disponible: http://www.minenergia.cl/techossolares/wp-content/uploads/2017/04/Reporte-de-Costos-de-Adjudicacion-2018-233x300.jpg. [20]Löhr W, Gauer K, Serrano N, Zamorano A. Igarss 2014. Eficiencia Energética en Hospitales Públicos. Editorial GTZ- Dalkia. Santiago de Chile. [21]Smith M, De Titto E. Hospitales sostenibles frente al cambio climático: huella de carbono de un hospital público de la ciudad de Buenos Aires. Revista Argentina Salud Pública. 2018; 9(36): 7-13. Disponible: http://rasp.msal.gov.ar/rasp/articulos/volumen36/7-13.pdf. [22]Chung J, Meltzer, D. Estimate of the carbon footprint of the US health care sector. Jama. 2009; 302(18):1970-1972. Disponible: https://jamanetwork.com/journals/jama/article-abstract/184856. [23]Nope A, García R, Bobadilla A. Método para la implementación de sistemas solares activos en establecimientos hospitalarios, estudio de caso en el hospital clínico del sur, Concepción, Chile. En Proceedings of the 3rd International Congress on Sustainable Construction and Eco-Efficient Solutions. Sevilla. 2017; 451-464. Disponible: https://idus.us.es/xmlui/handle/11441/58969. [24]Compañía General de Electricidad, Tarifa de Suministro. 2018 Disponible: http://www.cge.cl/wp-content/uploads/2019/08/Publicacion-CGE-2019-08-01-Suministro-electrico.pdf. [25]Ministerio de Desarrollo Social, Precio Social del Carbono. 2018. Disponible: http://sni.ministeriodesarrollosocial.gob.cl/download/precio-social-co2-2017/?wpdmdl=2406.

High levels of reactive oxygen species (ROS: superoxide, hydroxyl, hydrogen peroxide, nitric oxide, peroxynitrile) endanger sperm motility, viability, and function by interaction with membrane lipids, proteins, and nuclear and mitochondrial DNA (Sikka 2004 J. Androl. 25, 5–18). ROS generation has a significant negative effect on the fertilization rate after IVF, and so measurement of ROS levels in semen specimens before IVF may be useful in predicting the IVF outcome (Agarwal et al. 2005 Fertil. Steril. 84, 228–231). Several compounds of the antioxidant systems have been identified in the epididymal environment, spermatozoa, and seminal plasma. The antioxidants carnosine, L-histidine (Ducci et al. 2006 Pol. J. Vet. Sci. 9, 159–163), and taurine (Van der Horst and Grooten 1966 Biochim. Biophys. Acta. 117, 495–497) have been detected in boar semen and added to the extender in freezing procedures in several species. The main objective of this study was to evaluate the effect of carnosine, L-histidine, and taurine supplementation of the extender on boar sperm functionality as measured by sperm motility during computer-assisted semen analysis (CASA) and by IVF ability using mature oocytes, as previously described (Selles et al. 2003 Reprod. Domest. Anim. 38, 66–72). The sperm-rich fraction from mature fertile boars was diluted with isothermal Beltsville thawing solution (BTS) extender. Diluted semen was placed at 15�C and centrifuged at 800g for 10 min. The semen pellet was resuspended with BTS supplemented by 5 mm of carnosine, L-histidine, or taurine or not supplemented (control) to provide 75 � 106 spermatozoa mL–1 and stored at 15�C for 24 h (IVF assay), or 48 or 120 h (for CASA assay). We observed that the motility parameters were affected by storage time and that the addition of taurine increased the motility at 48 h of storage. Alternately, the addition of L-histidine to the extender reduced significantly the motility parameters after 120 h. The results showed that the addition of L-histidine induced a significant (P ≤ 0.01) decrease of the penetration rate (L-histidine 75.8% v. control 89.9%) and the number of sperm per oocyte penetrated (L-histidine 3.1 v. control 4.1). The rate of male pronuclear formation was not affected by the addition of antioxidants to the extender (over 85% in all cases). The addition of carnosine and taurine had no effect on the IVF parameters. In conclusion the antioxidants carnosine, taurine, and L-histidine affect sperm functionality differently, and further studies are necessary to elucidate what changes in sperm function take place during storage and the mechanisms by which these antioxidants exert their effects. This work was supported by Italian-Spanish research project HI2005-0165 and AGL2006-03495.

European hazelnut (Corylus avellana L.) is an emerging crop for export, mainly in southern Chile. Stem cankers and dieback of twigs on six-year-old European hazelnut cultivar Barcelona were observed during the 2012 growing season on plantations in Panguipulli (39° 38′ 37.12″ S and 72° 20′ 10.87″ W), Region de Los Rios, Chile. The incidence has been variable according to the place of plantation; it was estimated at approximately 15%. Cankers were characterized by brownish-gray and brown to reddish discoloration of the vascular stem system. Hazelnut plants between 1 and 3 years old developed stem basal canker, especially at conditions of high humidity and overpopulation of weeds; at critical conditions, the affected plants generally die. Small pieces of cankered stems, selected from 10 European hazelnuts, were surface sterilized in 0.5% sodium hypochlorite for 2 min and rinsed twice in sterile distilled water prior to incubation in a humid chamber for 7 days (25 ± 2°C) to stimulate production of reproductive bodies. Black sub-epidermal perithecia with unitunicate, cylindrical-clavate, 8-spored asci (n = 20) were obtained. Ascospores were septated, hyaline, multigutulate, and slightly constricted at the septum, the average measurements were (n = 20) 13.4 ± 0.6 μm × 3.9 ± 0.2 μm. The ascospores were transferred to potato dextrose agar (PDA) and incubated for 6 days at 25°C in the dark, then hyphal tips were transferred to fresh PDA and obtained a mycelia with white, cottony, and sparse colonies. Pycnidia and smooth, unicellular, hyaline, and biguttulate alpha conidia of 6.1 to 7.2 μm × 2.8 to 3.1 μm (n = 40) were observed. Beta conidia were not observed in culture media. Mature pycnidia were also detected on hazelnut shells remaining on the soil from the previous season. The identification of the species (isolate IMI-501237) was confirmed at CABI, United Kingdom, using an internal transcribed spacer (ITS), rDNA, BLASTn analysis of the 524-bp fragment, and showed 100% identity with Diaporthe australafricana Crous & J.M. van Niekerk (accessions KC343039, KC343038). These molecular and morphological characteristics were similar that reported from Vitis vinifera (2) and Chilean blueberry (3). The sequence obtained was deposited in GenBank (Accession No. JX316218.1). A pathogenicity test was conducted with isolate IMI-501237 on four 1-year-old plants from the hazelnut cultivar Barcelona. Plants were maintained in individual bags in greenhouse conditions (14/10 h dark/light, 20°C; 70% relative humidity). Prior to inoculation, plant tissues were surface disinfected with 2% sodium hypochlorite and rinsed with sterile distilled water. Each plant was inoculated at fresh wound sites on three stems and three vegetative buds on twigs. The inoculum consisted of an agar plug with mycelia (5 mm) from the edge of an actively growing colony cultured on PDA for 6 days. Each inoculation was covered with moistened cotton and sealed with Parafilm; a control plant was inoculated in the same way with agar only. After 30 days, necrotic lesions and discolored vascular tissue were only observed on inoculated stems and twigs. Symptomatic tissues were removed and incubated on PDA. D. australafricana was consistently recovered from these tissues, satisfying Koch's postulates. The control plant showed no symptoms of the disease. D. australafricana were previously reported on Vitis vinifera in Australia and South Africa (2,4), and Vaccinium corymbosum in Chile (1,3). To our knowledge, this is the first report of Diaporthe australafricana on Corylus avellana cultivar Barcelona in worldwide. References: (1) K. Elfar et al. Plant Dis. 97:1042, 2013. (2) R. Gomes et al. Persoonia 31:1, 2013. (3) B. Latorre et al. Plant Dis. 96:5, 2012. (4) J. M. van Niekerk et al. Australas. Plant Pathol. 34:27, 2005.

Background:The Rotterdam Early Arthritis Cohort (REACH) rule [1] and Clinical Arthritis RulE (CARE) [2] are both evidence-based and easy-to-use methods developed to identify the presence of inflammatory arthritis (IA) in patients suspected by their general practitioner (GP). However, the clinical utility of both models in daily clinical practice in an independent primary care setting has not yet been established. While developed for recognizing IA, we believe that it is also important that the broader spectrum of inflammatory rheumatic diseases (IRDs) is correctly classified from primary care, to facilitate appropriate referral towards outpatient rheumatology clinics.Objectives:The primary objective was to determine the diagnostic performance and clinical utility of the REACH and CARE referral rules in identifying IA in an independent population of unselected suspected patients from primary care. Secondly we will assess the diagnostic performance and clinical utility of both models in identifying IRDs.Methods:This prospective observational diagnostic study consisted of adults newly suspected by their GP for the need of referral to the rheumatology outpatient clinic of the Maasstad Hospital in Rotterdam. Primary outcome was IA, consisting of rheumatoid arthritis, axial spondylitis and psoriatic arthritis. Secondary outcome was IRD, defined as IA plus arthritis in systemic disorders such as systemic lupus erythematosus, systemic sclerosis and morbus sjögren. Rheumatologist diagnosis was used as gold standard. To evaluate the clinical performance of the REACH and CARE referral rules in this population, diagnostic accuracy measures were investigated using the Youden index (J) [3]. Moreover, a net benefit approach [4] was used to determine clinical utility of both rules when compared to usual care.Results:This study consisted of 250 patients (22.8% male) with a mean age of 50.8 years (SD 13.9 years). In total 42 (17%) patients were diagnosed with IA and 55 (22%) with an IRD. Figure 1 presents the diagnostic performance in IA (Figure 1A) and in IRD (Figure 1B). For the primary outcome, the REACH model shows an AUC of 0.72 (95% CI 0.64-0.80) and the optimal cut-off point is indicated (J). The CARE model shows an AUC of 0.82 (95% CI 0.75-0.88) and at J there is a somewhat higher sensitivity and specificity. When taking the broader spectrum of IRDs as outcome, the AUC was 0.66 (95% CI 0.58-0.74) for the REACH and 0.76 (95% CI 0.69-0.83) for the CARE model. The net benefit analysis with either IA or IRD as outcome showed that the CARE was of the highest clinical value when compared to usual care.Conclusion:Both the REACH and CARE model showed a good diagnostic performance for detecting IA in an independent population of unselected suspected patients from primary care. Although not specifically developed to recognize the entire spectrum of IRDs, the CARE shows a good performance in doing so. When evaluating clinical utility, we see that both rules have a net benefit in recognizing IA as well as IRDs compared to usual care, however the CARE shows superiority over the REACH. By using the CARE, over half of all suspected patients can be withheld from expensive outpatient rheumatology care, implied by the high specificity of 70%. These results support the idea that incorporating these easy-to-use methods into primary care could lead to providing patients the right care at the right place and improving value based health care.References:[1]ten Brinck RM, van Dijk BT, van Steenbergen HW, le Cessie S, Numans ME. Development and validation of a clinical rule for recognition of early inflammatory arthritis. BMJ Open; 2018: 8[2]Alves, C. Improving early referral of inflammatory arthritis. In Early detection of patients at risk for rheumatoid arthritis – a challenge for primary and secondary care; 2015: 27-38 Ridderkerk, the Netherlands.[3]Fluss R, Faraggi D, Reiser B. Estimation of the Youden Index and its associated cutoff point. Biom J; 2005: 47(4): 458-472[4]Vickers AJ, Elkin EB. Decision curve analysis: a novel method for evaluating prediction models. Med Decis Making; 2006: 26(6): 565-574Disclosure of Interests:None declared

Abstract. Trends in air quality across the Northern Hemisphere over a 21-year period (1990–2010) were simulated using the Community Multiscale Air Quality (CMAQ) multiscale chemical transport model driven by meteorology from Weather Research and Forecasting (WRF) simulations and internally consistent historical emission inventories obtained from EDGAR. Thorough comparison with several ground observation networks mostly over Europe and North America was conducted to evaluate the model performance as well as the ability of CMAQ to reproduce the observed trends in air quality over the past 2 decades in three regions: eastern China, the continental United States and Europe. The model successfully reproduced the observed decreasing trends in SO2, NO2, 8 h O3 maxima, SO42− and elemental carbon (EC) in the US and Europe. However, the model fails to reproduce the decreasing trends in NO3− in the US, potentially pointing to uncertainties of NH3 emissions. The model failed to capture the 6-year trends of SO2 and NO2 in CN-API (China – Air Pollution Index) from 2005 to 2010, but reproduced the observed pattern of O3 trends shown in three World Data Centre for Greenhouse Gases (WDCGG) sites over eastern Asia. Due to the coarse spatial resolution employed in these calculations, predicted SO2 and NO2 concentrations are underestimated relative to all urban networks, i.e., US-AQS (US – Air Quality System; normalized mean bias (NMB) = −38% and −48%), EU-AIRBASE (European Air quality data Base; NMB = −18 and −54%) and CN-API (NMB = −36 and −68%). Conversely, at the rural network EU-EMEP (European Monitoring and Evaluation Programme), SO2 is overestimated (NMB from 4 to 150%) while NO2 is simulated well (NMB within ±15%) in all seasons. Correlations between simulated and observed O3 wintertime daily 8 h maxima (DM8) are poor compared to other seasons for all networks. Better correlation between simulated and observed SO42− was found compared to that for SO2. Underestimation of summer SO42− in the US may be associated with the uncertainty in precipitation and associated wet scavenging representation in the model. The model exhibits worse performance for NO3− predictions, particularly in summer, due to high uncertainties in the gas/particle partitioning of NO3− as well as seasonal variations of NH3 emissions. There are high correlations (R > 0.5) between observed and simulated EC, although the model underestimates the EC concentration by 65% due to the coarse grid resolution as well as uncertainties in the PM speciation profile associated with EC emissions. The almost linear response seen in the trajectory of modeled O3 changes in eastern China over the past 2 decades suggests that control strategies that focus on combined control of NOx and volatile organic compound (VOC) emissions with a ratio of 0.46 may provide the most effective means for O3 reductions for the region devoid of nonlinear response potentially associated with NOx or VOC limitation resulting from alternate strategies. The response of O3 is more sensitive to changes in NOx emissions in the eastern US because the relative abundance of biogenic VOC emissions tends to reduce the effectiveness of VOC controls. Increasing NH3 levels offset the relative effectiveness of NOx controls in reducing the relative fraction of aerosol NO3− formed from declining NOx emissions in the eastern US, while the control effectiveness was assured by the simultaneous control of NH3 emission in Europe.

Section 211(3) of the Constitution of the Republic of South Africa, 1996 provides that no recognition of customary norms may be upheld if such norms are in conflict with either the constitution or any other law that deals specifically with customary law: “The courts must apply customary law when that law is applicable, subject to the Constitution and any legislation that specifically deals with customary law.” The current Recognition of Customary Marriages Act 120 of 1998 deals explicitly with the recognition of customary marriages which are concluded in accordance with customary law (s 1). Customary law is defined as the “customs and usages traditionally observed among the indigenous African peoples of South Africa and which form part of the culture of those peoples”. It follows from a further reading of section 1 that a customary marriage is reserved for those indigenous African peoples who observe such customs and usages. It is provided in section 10(4) that “[d]espite subsection (1), no spouse of a marriage entered into under the Marriage Act, 1961, is, during the subsistence of such marriage, competent to enter into any other marriage”. This must be read with the definitions contained in section 1: “‘customary law’ means the customs and usages traditionally observed among the indigenous African peoples of South Africa and which form part of the culture of those peoples; ‘customary marriage’ means a marriage concluded in accordance with customary law”. Without the requisite legal competency, no legal subject can enter into any relationship to which the law may attach any consequences. Nobody can enter into a customary marriage if any of the presumed future spouses is already in a civil marriage according to the Marriage Act 25 of 1961, not even if the two parties are married to each other. According to the custom of various indigenous nations, if a man enters into a valid customary marriage with a woman who had never been married before but who is the mother of children born out of wedlock (spurii), the metaphor applies that he “who takes the cow also acquires the calf”. He will as part and parcel of the lobola ceremony be seen as the adopting stepfather of his wife’s children, with all the accompanying consequences. He will automatically be responsible for the future maintenance of those children as his adoptive children and they will acquire all rights and privileges that are bestowed on a child, including the right to inheritance and the right to his family name. As a consequence of this new relationship, all legal ties with the biological father of the adopted child are severed and the biological father will no longer be responsible for the maintenance of his offspring. In January 2019 an erstwhile law professor from UNISA who still retained his German citizenship, was gravely ill and cared for on life-support at a hospital in the Pretoria district. While in hospital, he tied the marriage knot with Miss Vilakazi, a Zulu woman with whom he had been in a relationship for the past five years. Miss Vilakazi was a spinster, but she had a Zulu daughter who was born out of wedlock more than eight years previously out of a relationship with an erstwhile Zulu lover. This child had been in the care of her maternal grandmother in Natal and, according to Zulu customary norms, was considered part of the house of her maternal grandfather, Vilakazi. She consequently carried the name Vilakazi as her registered surname on her official birth certificate. The marriage, which was conducted on 29 January 2019 in the hospital in Pretoria, was concluded with adherence to all the requirements of Act 25 of 1961. The civil marriage was duly registered as such. The late professor passed away in the hospital barely three weeks later on 19 February 2019. Less than 24 hours before the demise of the professor a purported customary marriage was concluded, apparently on behalf of the professor with the recently married Mrs Schulze by proxy by a friend of his in the Newcastle district in Natal after having paid R60 000 as ilobolo. The ceremony was concluded with the ceremonial slaughtering of the prescribed goat. However, during this ceremony the groom was not present but on life support in a Pretoria hospital and not necessarily compos mentis – the court was told that he was represented by a friend. Zulu customary law, however, does not recognise a marriage concluded by proxy with a substitude bridegroom as was known in Roman-Dutch law as “a wedding with the glove”. Neither the Marriage Act nor the Recognition of Customary Marriages Act, however, recognises a second marriage after the conclusion of a civil marriage by any of the purported newly weds – even if both “spouses” had been present in person. The mother of the late Professor Schulze, after his demise in South Africa, amended her last will in Germany and appointed her lifelong partner as sole beneficiary of her significant estate. She passed away in Germany in October 2019. In November 2019 the recently married Mrs Schulze, on behalf of her minor daughter, successfully approached the high court in Pietermaritzburg, where Zaca AJ issued an order compelling the South African department of home affairs to issue the daughter with a new birth certificate that reflects the late Professor Schulze as her father. Notwithstanding the unease of the officials at home affairs with this court order, the minister of home affairs, Mr Motsoaledi, personally intervened in August 2020 and the new birth certificate was issued as requested. Relying on this newly issued birth certificate, the applicant claims an amount of not less than R8 million in Germany from the estate of the late mother of Professor Schulze. For this purpose, the applicant relies on a principle in German law, the Pflichtteilsanspruch, according to which any descendant of the deceased has a right to a prescribed portion, a so-called legitimate portion of the estate, if not mentioned or sufficiently bestowed in the last will. This raises a number of seriously flawed legal arguments that are analysed in this article. It is submitted that the perceived lobola marriage ceremony conducted on behalf of the late professor on 18 February 2019 in Newcastle, less than 24 hours before his demise, is void because of the explicit constitutional provision and the relevant section 10(4) of the Recognition of Customary Marriages Act 120 of 1998, which excludes any competency to enter into a customary marriage if any of the parties involved is already married. At the date of the perceived lobola ceremony, Mrs Schulze had already been civilly married to Professor Schulze for more than three weeks and thus both spouses lacked the necessary competency to enter into a valid customary marriage. Whether a valid customary marriage could have been concluded at all with a man who did not live according to the customs and usages of the Zulu, is also highly questionable. Because the perceived lobola marriage is a nullity, no legal consequences can flow from this nullity and the so-called customary adoption of the daughter (“the calf with the cow”) is a nullity too. At no stage was any of the requirements for a valid adoption as governed by the Children’s Act 38 of 2005 adhered to. The minister of home affairs should have immediately given notice of appeal after the unconvincing judgment of Zaca AJ was handed down in January 2020. As the responsible minister, he should guard the upholding of the constitution and the applicable legal provisions unambiguously contained in the relevant section 10(4) of Act 120 of 1998. It is a pity that the so-called adherence to the principles of the “rule of law” is not even paid lip service in this case. Bennett, as a renowned expert on customary law, correctly pointed out that the legal orders are not unconnected. It may never be assumed that the people concerned are unaware of how to manipulate the resources offered them by legal pluralism (A Sourcebook of African Customary Law for Southern Africa (1991) 50).

Abstract In CD34-positive acute myeloid leukemia (AML), the leukemia-initiating event is thought to occur in the CD34+CD38− stem cell compartment. Survival of these cells after chemotherapy hypothetically results in minimal residual disease (MRD) and leads to relapse. C-type lectin-like molecule 1 (CLL-1) could serve as a possible target for therapy, as we previously showed that it is expressed on malignant CD34+CD38− cells at diagnosis and not on normal CD34+CD38− cells (van Rhenen et al., Blood106: 6a, 2005). CLL-1 expression is also present on malignant CD34+CD38− cells after chemotherapy. In the present study we investigated whether other antigens are also aberrantly expressed on CD34+CD38− cells in AML patients at diagnosis which would allow detection of AML CD34+CD38− cells in remission bone marrow. Such would offer opportunities for patient risk stratification and guidance of therapeutic intervention. Flowcytometry was performed on bone marrow CD34+CD38− cells in AML at diagnosis, after chemotherapy and in normal (NBM) and regenerating (RBM) bone marrow. Antibodies were anti-CD34, anti-CD45 and anti-CD38 together with antibodies against CLL-1, CD2, CD5, CD7, CD11b, CD19 and CD56, apart from CLL-1 all used as leukemia-associated phenotypes (LAPs) in immunophenotypic MRD detection (Feller et al., Leukemia8:1380, 2004; further referred to as “whole blast” MRD). In diagnosis AML marker expression on CD34+CD38- cells was scored as <50% or >50%, because at least 50% expression is needed for accurate measurements of malignant CD34+CD38- cells after chemotherapy. At diagnosis, 60/77 AML samples were CD34-positive. A reliable number of CD34+CD38- events (>20) could be measured in 56/60 cases. CLL-1 expression >50% was observed in 15/60 cases, LAP expression in 9/60 cases and both CLL-1 and LAP in 8/60 cases. Altogether, in 32/60 CD34-positive cases, detection of malignant CD34+CD38- cells was possible. In NBM (n=4) as well as in RBM, the CD34+CD38- cells did show low CLL-1 (n=6) and low LAP (n=2, for all antigens) expression (all <3%). For three AML samples in which a leukemia-specific translocation was present, FISH analysis showed the translocation in CD34+CD38-LAP+ cells. Therefore, CLL-1 and/or LAP staining might enable to accurately discriminate between residual normal and malignant CD34+CD38- cells after chemotherapy. Firstly, we found that irrespective of the aberrant phenotype or the time point of analysis the frequency of residual malignant CD34+CD38- cells significantly correlated with “whole blast” MRD (r=0.42, p=0.008, n=38). Secondly the ratio between malignant and normal CD34+CD38- cells (stem cell ratio) was found to parallel both CD34+CD38- cell frequency and “whole blast” MRD. Both parameters, similar to “whole blast” MRD, parallelled clinical outcome with decreases when entering remission, no changes in non-responders and increases with forthcoming relapses. In 3/60 cases in which “whole blast” MRD could not be used, stem cell MRD could be performed. Since the populations are so well defined [CD34+/CD38−/CD45dim/aberrant marker] stem cell MRD will require less extensive experience than currently used MRD frequency assessment. These advantages are expected to increase as the use of 5 or more fluorescence channels will improve the success of stem cell MRD. In conclusion it is possible to detect malignant stem cells in AML patients at diagnosis and after chemotherapy, which would offer opportunities for future patient risk stratification and guidance of therapeutic intervention.