Relevant bibliographies by topics / Wga-hrp

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Academic literature on the topic 'Wga-hrp'

Author: Grafiati
Published: 4 June 2021
Last updated: 3 February 2022

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Journal articles on the topic "Wga-hrp"

1

Arbab, M. A. R., T. Delgado, L. Wiklund, and N. Aa Svendgaard. "Brain Stem Terminations of the Trigeminal and Upper Spinal Ganglia Innervation of the Cerebrovascular System: WGA-HRP Transganglionic Study." Journal of Cerebral Blood Flow & Metabolism 8, no. 1 (February 1988): 54–63. http://dx.doi.org/10.1038/jcbfm.1988.8.

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The central projections of the nerve fibers innervating the middle cerebral and basilar arteries were investigated by transganglionic tracing of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP) in the rat. WGA-HRP was applied to the exposed basilar and/or middle cerebral arteries. Sections of the brain, trigeminal and upper spinal ganglia were reacted with tetramethylbenzidine for detection of the tracer. The results demonstrate that trigeminal neurons that innervate the middle cerebral artery project to the trigeminal main sensory nucleus, pars oralis, and the dorsocaudal two-fifths of pars interpolaris of the trigeminal brain stem nuclear complex. Terminals were also visible in the ipsilateral nucleus motorius dorsalis nervi vagi (dmnX) and in the lateral nucleus tractus solitarius (nTs) bilaterally at the level of the obex. The ventral periaqueductal gray, including the dorsal raphe and C2 dorsal horn, were also innervated by nerve fibers from the middle cerebral artery. Ipsilateral trigeminal rhizotomy prior to WGA-HRP application over the middle cerebral artery impeded the visualization of nerve terminations throughout the brain stem. Pretreatment with capsaicin reduced the density of labeled neurons and terminals within the trigeminal ganglion and the brain stem, respectively, following WGA-HRP application over the middle cerebral artery. Basilar artery fibers terminate in the C2 dorsal horn, the cuneate nuclei, dmnX, and nTs bilaterally. A few projections were also labeled in the ventral periaqueductal gray. Unilateral upper two spinal dorsal rhizotomy prior to WGA-HRP application over the exposed basilar artery resulted in terminal labeling within the C2 dorsal horn, the cuneate nucleus, dmnX, and nTs contralateral to the rhizotomy, whereas the ipsilateral side was devoid of any labeling. Bilateral superior cervical ganglionectomy prior to WGA-HRP administration to the middle cerebral and basilar arteries did not alter the visualization of nerve terminations throughout the brain stem.
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2

Gattone, V. H., C. F. Marfurt, and S. Dallie. "Extrinsic innervation of the rat kidney: a retrograde tracing study." American Journal of Physiology-Renal Physiology 250, no. 2 (February 1, 1986): F189—F196. http://dx.doi.org/10.1152/ajprenal.1986.250.2.f189.

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To determine the exact modalities involved in the innervation of the kidney, the present study used a nerve-tracing method with horseradish peroxidase-wheat germ agglutinin (HRP-WGA) as the tracer. Multiple injections of HRP-WGA were made in each of the left kidneys of 12 rats while another four had the HRP-WGA either dripped onto their intact renal mesothelial surface or injected intravascularly. After retrograde transport of the tracer to neurons of origin (i.e., 72-h survival), the rats were briefly perfusion fixed, tissue was removed, and cryostat sections were cut. The free-floating sections were reacted by the tetramethylbenzidine technique. Retrogradely labeled neurons were found in the celiac, bilateral inferior vagal (nodosal), and ipsilateral dorsal root (90% in T12-L1 DRG) ganglia. More labeled neurons were present in the combined vagal ganglia than in the combined DRG within each animal. This labeling was specific compared with the controls (HRP-WGA uptake via intraperitoneal or vascular routes). The celiac ganglion had many labeled neurons; however, no labeled neurons were seen in the dorsal motor nucleus of the vagus, nucleus solitarius, nucleus ambiguus, or any other brain stem structure after renal injections of HRP-WGA. This study has determined that the sympathetic nervous system (celiac ganglion) provides the only renal autonomic efferent (motor) innervation, and the nodosal (inferior vagal) ganglia appear to provide more renal sensory innervation than do the dorsal root ganglia.
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3

Iida, H., and Y. Shibata. "Delivery of lectin-labeled membrane to the trans-Golgi network and secretory granules in cultured atrial myocytes." Journal of Histochemistry & Cytochemistry 37, no. 12 (December 1989): 1885–92. http://dx.doi.org/10.1177/37.12.2479675.

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To examine whether and how internalized plasma membrane components are routed to the compartment of the biosynthetic-exocytic pathway in cultured atrial myocytes, the plasma membrane labeled with wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) was traced electron microscopically by cytochemical detection of HRP. The WGA-HRP label was internalized via a coated pit-small vesicle pathway and reached vacuoles and endosomes by 3 min. Labeled endosomes comprised vacuoles and tubular elements containing reaction product. By 15 min, similar tubular structures containing reaction product accumulated in the area of the trans-Golgi network (TGN). The labeled TGN consisted of interconnected tubular elements, which often connected to atrial granules containing reaction product. In contrast, neither native HRP nor Lucifer Yellow reached Golgi elements or atrial granules. These results suggest that a proportion of the plasma membrane labeled with WGA-HRP is delivered to endosomes, from which tubules might bud off to transfer the tracer molecules to the TGN, where the lectin conjugate and associated membranes are packaged into atrial granules.
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4

Liu, H., I. J. Llewellyn-Smith, and A. I. Basbaum. "Co-injection of wheat germ agglutinin-HRP and choleragenoid-HRP into the sciatic nerve of the rat blocks transganglionic transport." Journal of Histochemistry & Cytochemistry 43, no. 5 (May 1995): 489–95. http://dx.doi.org/10.1177/43.5.7730587.

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We report on the surprising loss of transganglionic and retrograde labeling in the spinal cord of the rat after co-injection of the tracers wheat germ agglutinin-HRP (WGA-HRP) and choleragenoid toxin-HRP (CTB-HRP) into the sciatic nerve. Injection of WGA-HRP alone produced a pattern of transganglionic label consistent with transport by small-diameter primary afferent fibers. Small cell bodies were labeled in the ipsilateral dorsal root ganglion (DRG) and there was dense terminal labeling in the superficial dorsal horn of the lumbar spinal cord. Injection of CTB-HRP alone produced a pattern of transganglionic labeling consistent with transport by large-diameter primary afferent fibers. Large cell bodies were labeled in the DRG and there was dense terminal labeling in the nucleus proprius (Laminae III-V) in the spinal cord. CTB-HRP also produced extensive retrograde labeling of ventral horn motor neurons. When the two tracers were co-injected, we found few labeled cells in the ipsilateral DRG and there was almost complete loss of transganglionic terminal labeling in the lumbar spinal cord. Retrograde labeling of motor neurons was also significantly reduced. Even when one of the tracers (e.g., WGA-HRP) was injected 24 hr after and up to 10 mm proximal to the site of the first tracer (e.g., CTB-HRP), an inhibitory interaction was detected. The labeling pattern was always characteristic of the first tracer injected.(ABSTRACT TRUNCATED AT 250 WORDS)
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5

Kressel, Michael. "Tyramide Amplification Allows Anterograde Tracing by Horseradish Peroxidase-conjugated Lectins in Conjunction with Simultaneous Immunohistochemistry." Journal of Histochemistry & Cytochemistry 46, no. 4 (April 1998): 527–33. http://dx.doi.org/10.1177/002215549804600413.

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Current protocols for a combined approach of anterograde tracing with carbocyanine dyes or horseradish peroxidase (HRP) conjugates and immunohistochemistry represent a compromise between sensitive detection of the tracer and the immunohistochemical procedure. Therefore, it was investigated whether the use of tyramide amplification allows sensitive anterograde tracing with wheat-germ agglutinin conjugated to horseradish peroxidase (WGA–HRP) in conjunction with simultaneous immunohistochemistry. Vagal afferents were anterogradely labeled by injection of WGA–HRP into the nodose ganglion of rats. By use of tyramide–biotin amplification, a dense fiber plexus of vagal afferents was visualized centrally in the nucleus of the solitary tract and in retrogradely labeled neurons in the dorsal vagal nucleus. In the esophagus and duodenum, large- and small-caliber vagal fibers and terminals could be demonstrated comparably to conventional tracing techniques using carbocyanine dyes or WGA–HRP and TMB histochemistry. Combination with immunohistochemistry could easily be done, requiring only one more incubation step, and did not result in loss of sensitivity of the tracing. With this method and con-focal microscopy, the presence of Ca binding proteins in vagal afferent terminals could be demonstrated. Tyramide amplification allows sensitive anterograde tracing with low background staining in conjunction with immunohistochemistry of intra-axonal markers.
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6

Monti-Graziadei, Ariella G., and Karen J. Berkley. "Effects of colchicine on retrogradely-transported WGA-HRP." Brain Research 565, no. 1 (November 1991): 162–66. http://dx.doi.org/10.1016/0006-8993(91)91749-q.

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7

Balin, B. J., and R. D. Broadwell. "Lectin-labeled membrane is transferred to the Golgi complex in mouse pituitary cells in vivo." Journal of Histochemistry & Cytochemistry 35, no. 4 (April 1987): 489–98. http://dx.doi.org/10.1177/35.4.2434560.

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Labeling of the Golgi complex with the lectin conjugate wheat germ agglutinin-horseradish peroxidase (WGA-HRP), which binds to cell surface membrane and enters cells by adsorptive endocytosis, was analyzed in secretory cells of the anterior, intermediate, and posterior lobes of mouse pituitary gland in vivo. WGA-HRP was administered intravenously or by ventriculo-cisternal perfusion to control and salt-stressed mice; post-injection survival times were 30 min-24 hr. Peroxidase reaction product was identified within the extracellular clefts of anterior and posterior pituitary lobes through 24 hr but was absent in intermediate lobe. Endocytic vesicles, spherical endosomes, tubules, dense and multivesicular bodies, the trans-most saccule of the Golgi complex, and dense-core secretory granules attached or unattached to the trans Golgi saccule were peroxidase-positive in the different types of anterior pituitary cells and in perikarya of supraoptico-neurohypophyseal neurons; endoplasmic reticulum and the cis and intermediate Golgi saccules in the same cell types were consistently devoid of peroxidase reaction product. Dense-core secretory granules derived from cis and intermediate Golgi saccules in salt-stressed supraoptic perikarya likewise failed to exhibit peroxidase reaction product. The results suggest that in secretory cells of anterior and posterior pituitary lobes, WGA-HRP, initially internalized with cell surface membrane, is eventually conveyed to the trans-most Golgi saccule, in which the lectin conjugate and associated membrane are packaged in dense-core secretory granules for export and potential exocytosis of the tracer. Endoplasmic reticulum and the cis and intermediate Golgi saccules appear not to be involved in the endocytic/exocytic pathways of pituitary cells exposed to WGA-HRP.
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8

Johnston, P. A., A. Stieber, and N. K. Gonatas. "A hypothesis on the traffic of MG160, a medial Golgi sialoglycoprotein, from the trans-Golgi network to the Golgi cisternae." Journal of Cell Science 107, no. 3 (March 1, 1994): 529–37. http://dx.doi.org/10.1242/jcs.107.3.529.

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We have reported that MG160, an intrinsic membrane sialoglycoprotein of the Golgi apparatus (GA), resides in the medial cisternae of the organelle (Gonatas et al. (1989) J. Biol. Chem. 264, 646–653). In order to resolve the question whether MG160 acquires sialic acid residues in the trans cisternae or trans-Golgi network (TGN) prior to its retrograde transport, we have examined the effects of brefeldin A (BFA) on the post-translational processing of MG160, and the distribution of internalized wheat germ agglutinin covalently linked with HRP (WGA-HRP), which labels the TGN (Gonatas et al. (1977) J. Cell Biol. 73, 1–13). In BFA-treated PC12 cells, MG160 acquires resistance to endo H, but fails to be sialylated. This effect occurs in parallel with the redistribution of MG160 into an ER compartment dispersed throughout the cytoplasm including the nuclear envelope, and the collapse of the WGA-HRP-labelled TGN into vesicles and tubules surrounding the centriole. These results suggest that MG160 is not sialylated in BFA-treated cells because it is sequestered from the sialyltransferase enzyme(s), presumably located in the TGN, and provide evidence supporting the hypothesis for a retrograde transport pathway that recycles resident GA proteins, including MG160, between the Golgi cisternae and the TGN. To examine further the above hypothesis we studied cells treated with BFA and then allowed to recover from the effect of the drug for various lengths of time. After 15 minutes of recovery, cisternae of the Golgi apparatus, typically found in the pericentriolar region, are labeled by both MG160 and WGA-HRP.(ABSTRACT TRUNCATED AT 250 WORDS)
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9

Gong, Suzhen, and Mark S. LeDoux. "Immunohistochemical detection of wheat germ agglutinin-horseradish peroxidase (WGA-HRP)." Journal of Neuroscience Methods 126, no. 1 (June 2003): 25–34. http://dx.doi.org/10.1016/s0165-0270(03)00055-4.

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10

Itaya, S. K. "Anterograde transsynaptic transport of WGA-HRP in rat olfactory pathways." Brain Research 409, no. 2 (April 1987): 205–14. http://dx.doi.org/10.1016/0006-8993(87)90703-7.

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Dissertations / Theses on the topic "Wga-hrp"

1

Kurimoto, Yasuo. "Cerebellotectal projection in the rat: anterograde and retrograde WGA-HRP study of individual cerebellar nuclei." Kyoto University, 1995. http://hdl.handle.net/2433/160725.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである
Kyoto University (京都大学)
0048
新制・課程博士
博士(医学)
甲第5999号
医博第1665号
新制||医||608(附属図書館)
UT51-95-D318
京都大学大学院医学研究科生理系専攻
(主査)教授 水野 昇, 教授 本田 孔士, 教授 川口 三郎
学位規則第4条第1項該当
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2

Cadusseau, Josette. "Proposition d'une nouvelle définition du noyau postérieur du thalamus chez le rat, d'après une étude hodologique." Poitiers, 1987. http://www.theses.fr/1987POIT2025.

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3

Ma, Wu. "Aspects structuraux et ultrastructuraux des projections spinales et trigeminales dans le thalamus et l'aire parabrachiale." Paris 6, 1987. http://www.theses.fr/1987PA066132.

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Book chapters on the topic "Wga-hrp"

1

Kuroda, R., A. Yorimae, Y. Yamada, Y. Furuta, and A. Kim. "Frontal Cingulotomy Reconsidered from a WGA-HRP and c-Fos Study in Cat." In Advances in Stereotactic and Functional Neurosurgery 11, 69–73. Vienna: Springer Vienna, 1995. http://dx.doi.org/10.1007/978-3-7091-9419-5_15.

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2

"WGA-HRP." In Encyclopedia of Pain, 4259. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-28753-4_202465.

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