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Journal articles on the topic 'White Rabbit PTP'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Fedotov, A., I. Orlov, and A. Antonov. "Precise clock synchronization for radio frequency positioning system based on White Rabbit PTP." Journal of Physics: Conference Series 1614 (August 2020): 012107. http://dx.doi.org/10.1088/1742-6596/1614/1/012107.

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2

Li, Jia-Zeng, and Eric Chun-Yet Lian. "Mechanism of Rabbit Platelet Agglutination Induced by Acidic Mucopolysaccharide Extracted from Stichopus japonicus Selenka." Thrombosis and Haemostasis 59, no. 03 (1988): 432–34. http://dx.doi.org/10.1055/s-0038-1647510.

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SummaryThe effects of acidic mucopolysaccharide extracted from sea cucumber (Stichopus japonicus Selenka) (SJAMP) on rabbit platelets were studied. Using citrated platelet-rich plasma (PRP), washed platelets, and formaldehyde fixed platelets from 10 New Zealand white rabbits, we investigated the effects of platelet inhibitors and various plasma and its fractions on SJAMP-induced agglutination. It was found that the tracing of platelet agglutination induced by SJAMP showed a single phase without a lag period. The lowest concentration of SJAMP required for the agglutination of rabbit platelets w

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D'Cruz, Osmond J., Barbara Waurzyniak, and Fatih M. Uckun. "Mucosal Toxicity Studies of a Gel Formulation of Native Pokeweed Antiviral Protein." Toxicologic Pathology 32, no. 2 (2004): 212–21. http://dx.doi.org/10.1080/01926230490274362.

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Pokeweed antiviral protein (PAP), a 29-kDa plant-derived protein isolated from Phytolacca americana, is a promising nonspermicidal broad-spectrum antiviral microbicide. This study evaluated the mucosal toxicity potential of native PAP in the in vivo rabbit vaginal irritation model as well as the in vitro reconstituted human vaginal epithelial tissue model. Twenty-two New Zealand white rabbits in 4 subgroups were exposed intravaginally to a gel with and without 0.01, 0.1, or 1.0% native PAP for 10 consecutive days. The dose of PAP used represented nearly 200- to 20,000 times its in vitro anti-H

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Spakova, Timea, Judita Amrichova, Jana Plsikova, et al. "A Preliminary Study Comparing Microfracture and Local Adherent Transplantation of Autologous Adipose-Derived Stem Cells Followed by Intraarticular Injection of Platelet-Rich Plasma for the Treatment of Chondral Defects in Rabbits." CARTILAGE 9, no. 4 (2017): 410–16. http://dx.doi.org/10.1177/1947603517713816.

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Objective This study aimed to compare microfracture and application of adipose-derived stem cells (ADSCs) by local adherent technique enhanced by platelet-rich plasma (PRP) to provide a new approach for the repair of cartilage defect. Design Full-thickness cylindrical defects were created in the medial femoral condyle in 9 New Zealand White rabbits (5 months old, 4.65 ± 0.20 kg). Two groups of rabbits ( n = 3) were either treated with ADSCs (Group 1) or the microfracture technique (Group 2) following intraarticular injection of PRP 3 times in weekly intervals. Rabbits in control group ( n = 3)

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LaPrade, Robert F., Laurie R. Goodrich, Jennifer Phillips, et al. "Use of Platelet-Rich Plasma Immediately After an Injury Did Not Improve Ligament Healing, and Increasing Platelet Concentrations Was Detrimental in an In Vivo Animal Model." American Journal of Sports Medicine 46, no. 3 (2017): 702–12. http://dx.doi.org/10.1177/0363546517741135.

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Background: Limited information in basic science and clinical trials exists to determine if ligament healing may be accelerated with the use of biological adjuvants, such as platelet-rich plasma (PRP). However, there has been widespread acceptance of PRP for use in clinical practice, despite an inadequate understanding of its biological mechanism of action. Purpose: To determine whether a single dose of PRP could accelerate ligament healing and correspondingly improve histological characteristics and biomechanical properties when injected immediately postoperatively into the injured medial col

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Slimi, Fathia, Wassim Zribi, Moez Trigui, et al. "The effectiveness of platelet-rich plasma gel on full-thickness cartilage defect repair in a rabbit model." Bone & Joint Research 10, no. 3 (2021): 192–202. http://dx.doi.org/10.1302/2046-3758.103.bjr-2020-0087.r2.

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Aims The present study investigates the effectiveness of platelet-rich plasma (PRP) gel without adjunct to induce cartilage regeneration in large osteochondral defects in a rabbit model. Methods A bilateral osteochondral defect was created in the femoral trochlear groove of 14 New Zealand white rabbits. The right knees were filled with PRP gel and the contralateral knees remained untreated and served as control sides. Some animals were killed at week 3 and others at week 12 postoperatively. The joints were harvested and assessed by Magnetic Resonance Observation of Cartilage Repair Tissue (MOC

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Hernandez, L. A., K. J. Peevy, A. A. Moise, and J. C. Parker. "Chest wall restriction limits high airway pressure-induced lung injury in young rabbits." Journal of Applied Physiology 66, no. 5 (1989): 2364–68. http://dx.doi.org/10.1152/jappl.1989.66.5.2364.

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High peak inspiratory pressures (PIP) during mechanical ventilation can induce lung injury. In the present study we compare the respective roles of high tidal volume with high PIP in intact immature rabbits to determine whether the increase in capillary permeability is the result of overdistension of the lung or direct pressure effects. New Zealand White rabbits were assigned to one of three protocols, which produced different degrees of inspiratory volume limitation: intact closed-chest animals (CC), closed-chest animals with a full-body plaster cast (C), and isolated excised lungs (IL). The

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Pazzini, Josiane M., Andrigo B. De Nardi, Rafael R. Huppes, et al. "Method to obtain platelet-rich plasma from rabbits (Oryctolagus cuniculus )." Pesquisa Veterinária Brasileira 36, no. 1 (2016): 39–44. http://dx.doi.org/10.1590/s0100-736x2016000100007.

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Abstract: Platelet-rich plasma (PRP) is a product easy and inxpesnsive, and stands out to for its growth factors in tissue repair. To obtain PRP, centrifugation of whole blood is made with specific time and gravitational forces. Thus, the present work aimed to study a method of double centrifugation to obtain PRP in order to evaluate the effective increase of platelet concentration in the final product, the preparation of PRP gel, and to optimize preparation time of the final sample. Fifteen female White New Zealand rabbits underwent blood sampling for the preparation of PRP. Samples were sepa

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Negrini, Daniela, Olav Tenstad, Alberto Passi, and Helge Wiig. "Differential degradation of matrix proteoglycans and edema development in rabbit lung." American Journal of Physiology-Lung Cellular and Molecular Physiology 290, no. 3 (2006): L470—L477. http://dx.doi.org/10.1152/ajplung.00310.2005.

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The specific role of solid extracellular matrix components in opposing development of pulmonary interstitial edema was studied in adult anesthetized rabbits by challenging the lung parenchyma with an intravenous injection of a bolus of collagenase or heparanase. In 10 rabbits, pulmonary interstitial pressure (Pip) was measured by micropuncture in control and up to 3 h after collagenase or heparanase intravenous injection. With respect to control (Pip= −9.3 ± 1.5 cmH2O, n = 10), both treatments caused a significant increase of Pip and of the wet weight-to-dry weight lung ratio. However, while t

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Edward, Mouli, Henry Dominica, Ferdiansyah Mahyudin, and Fedik Abdul Rantam. "DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT." (JOINTS) Journal Orthopaedi and Traumatology Surabaya 9, no. 2 (2020): 34. http://dx.doi.org/10.20473/joints.v9i2.2020.34-54.

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Background: Bone defects to date have been a significant problem in the Orthopedics field. Hydroxyapatite is a bone graft that is often chosen if it has osteoconductive properties. Platelet-rich plasma (PRP) has a higher platelet concentration than the concentration in normal blood, capable of providing many bioactive molecules in physiological proportions. Hydroxyapatite given freeze-dried PRP is expected to create a graft that can strengthen the matrix while promoting osteoinduction.Methods: This study compares the effects of regeneration on the bone between bovine hydroxyapatite (BHA) and b

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Liu, Baoru, Wenzhi Chen, Jingwei Jiang, et al. "Treatment Effect of Low-Intensity Pulsed Ultrasound on Benzene- and Cyclophosphamide-Induced Aplastic Anemia in Rabbits." Physical Therapy 99, no. 11 (2019): 1443–52. http://dx.doi.org/10.1093/ptj/pzz074.

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Abstract Background Transplantation and immunosuppressive therapies are the available treatments for aplastic anemia; however, each strategy has its advantages and disadvantages. Objective The aim of this study was to find a new strategy for aplastic anemia treatment. Design This was an experimental and comparative study. Methods The aplastic anemia model was established by injecting rabbits with benzene and cyclophosphamide. The rabbits with aplastic anemia were divided into low-intensity pulsed ultrasound (LIPUS) and control groups. The distal femoral metaphysis of rabbits in the LIPUS group

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Penrose, Donna M., and Bernard R. Glick. "Production of antibodies against sorghum leaf phosphoenolpyruvate carboxylase monomer and their use in monitoring phosphoenolpyruvate carboxylase levels in sorghum tissues." Biochemistry and Cell Biology 64, no. 12 (1986): 1234–41. http://dx.doi.org/10.1139/o86-162.

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The enzyme phosphoenolpyruvate carboxylase (PEP-C) was purified from leaves of the C4 plant Sorghum bicolor. The purified enzyme had a specific activity of 30 U/mg protein and an apparent molecular mass of 415 000 daltons. Rabbit antibodies produced against purified PEP-C monomer were used to develop an enzyme-linked immunosorbent assay for PEP-C. Titration of rabbit antiserum against both crude and purified PEP-C indicated a 10-fold difference in response, suggesting a 10-fold purification of the enzyme, consistent with the 10-fold purification indicated by densitometric traces of sodium dode

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Schneppendahl, J., T. T. Lögters, M. Sager, et al. "Treatment of a diaphyseal long-bone defect with autologous bone grafts and platelet-rich plasma in a rabbit model." Veterinary and Comparative Orthopaedics and Traumatology 28, no. 03 (2015): 164–71. http://dx.doi.org/10.3415/vcot-14-05-0079.

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Summary Introduction: Large bone defects are a therapeutic challenge to surgeons and are often associated with a high morbidity. The use of autologous cancellous bone graft represents an essential therapeutic option and is considered the gold standard. However, the use of platelet-rich plasma (PRP) for improving bone defect healing has been discussed controversially. The aim of this study was to evaluate the treatment of a diaphyseal long-bone defect in a rabbit model with a combination of PRP and autologous cancellous bone. Material and methods: A monocortical long-bone defect in the radial d

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Gutierrez-Rivas, Jose Luis, Felipe Torres-Gonzalez, Eduardo Ros, and Javier Diaz. "Enhancing White Rabbit Synchronization Stability and Scalability Using P2P Transparent and Hybrid Clocks." IEEE Transactions on Industrial Informatics 17, no. 11 (2021): 7316–24. http://dx.doi.org/10.1109/tii.2021.3054365.

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Deprés-Tremblay, Gabrielle, Anik Chevrier, Martyn Snow, Scott Rodeo, and Michael D. Buschmann. "Freeze-dried chitosan-platelet-rich plasma implants improve supraspinatus tendon attachment in a transosseous rotator cuff repair model in the rabbit." Journal of Biomaterials Applications 33, no. 6 (2018): 792–807. http://dx.doi.org/10.1177/0885328218811386.

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Rotator cuff tears result in shoulder pain, stiffness, weakness and loss of motion. After surgical repair, high failure rates have been reported based on objective imaging and it is recognized that current surgical treatments need improvement. The aim of the study was to assess whether implants composed of freeze-dried chitosan (CS) solubilized in autologous platelet-rich plasma (PRP) can improve rotator cuff repair in a rabbit model. Complete tears were created bilaterally in the supraspinatus tendon of New Zealand White rabbits ( n = 4 in a pilot feasibility study followed by n = 13 in a lar

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Yang, Q. H., and S. J. Lai-Fook. "Effect of lung inflation on regional lung expansion in supine and prone rabbits." Journal of Applied Physiology 71, no. 1 (1991): 76–82. http://dx.doi.org/10.1152/jappl.1991.71.1.76.

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At functional residual capacity, lung expansion is more uniform in the prone position than in the supine position. We examined the effect of positive airway pressure (Paw) on this position-dependent difference in lung expansion. In supine and prone rabbits postmortem, we measured alveolar size through dependent and nondependent pleural windows via videomicroscopy at Paw of 0 (functional residual capacity), 7, and 15 cmH2O. After the chest was opened, alveolar size was measured in the isolated lung at several transpulmonary pressures (Ptp) on lung deflation. Alveolar mean linear intercept (Lm)

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Tseng, Ching-Li, Po-Chih Hsu, Lin-Gwei Wei, et al. "EFFECT OF PLATELET-RICH PLASMA MIXED WITH A POLYMERIC BONE FILLING MATERIAL ON SINUS FLOOR AUGMENTATION IN RABBITS." Biomedical Engineering: Applications, Basis and Communications 25, no. 05 (2013): 1340004. http://dx.doi.org/10.4015/s1016237213400048.

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Sinus augmentation is a common approach for patients with severe alveolar ridge atrophy but an ideal material to increase the bone volume for dental implantation is still needed. The present study evaluated the effect of a newly developed polymeric bone-filling powder (formed from acrylic acid and N-isopropylacrylamide, ANa) mixed with platelet-rich plasma (PRP) for bone generation in a rabbit model of sinus augmentation. A total of 12 New Zealand White rabbits were randomly divided into three groups based on filling material. All animals underwent a bilateral maxillary sinus augmentation. PRP

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Utomo, Dwikora Novembri, Fedik Abdul Rantam, Ferdiansyah, and Purwati. "Regeneration Mechanism of Full Thickness Cartilage Defect Using Combination of Freeze Dried Bovine Cartilage Scaffold - Allogenic Bone Marrow Mesenchymal Stem Cells - Platelet Rich Plasma Composite (SMPC) Implantation." Journal of Biomimetics, Biomaterials and Biomedical Engineering 31 (March 2017): 70–82. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.31.70.

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Cartilage defect has become serious problem for orthopaedic surgeon and patients because of its difficult healing that might occur when articular cartilage damage never reach subchondral layer. In this study, we used combination of freeze dried bovine cartilage (FDBC) scaffold, bone marrow mesenchymal stem cells (BM-MSCs), and platelet rich plasma (PRP) composite (SMPC) implanted in full thickness cartilage defect. This study is to explain its regeneration mechanism. This is true experimental research with post-test only control group design using New Zealand White Rabbit. 50 rabbits is divide

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Ahmed, Dawlat Mostafa, and Samia Soliman Omar. "Cementing Line Configuration of Bioactive Engineered Zirconia Implants (In Vivo Histological Study)." Key Engineering Materials 786 (October 2018): 236–47. http://dx.doi.org/10.4028/www.scientific.net/kem.786.236.

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Background: Osseointegration of dental implant is a challenging issue for those who need to restore a missing tooth, a matter that necessitates wide range of research on different implant manufacturing, modification and bioactive coating materials.Aim of the study: Investigation of cementing line configuration around osseointegrated engineered nano-porous zirconia implants coated with bioactive autologous HA, platelet rich plasma (PRP) and bovine HA (Bio-oss) coatings in rabbit model.Materials and methods: Zirconia implants (cylinders 3.7 mm x 8 mm) were milled and sintered according to manufa

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Muhanna, Nidal, Harley H. L. Chan, Jason L. Townson, et al. "Photodynamic therapy enables tumor-specific ablation in preclinical models of thyroid cancer." Endocrine-Related Cancer 27, no. 2 (2020): 41–53. http://dx.doi.org/10.1530/erc-19-0258.

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The incidence of differentiated thyroid cancer has increased significantly during the last several decades. Surgical resection is the primary treatment for thyroid cancer and is highly effective, resulting in 5-year survival rates greater than 98%. However, surgical resection can result in short- and long-term treatment-related morbidities. Additionally, as this malignancy often affects women less than 40 years of age, there is interest in more conservative treatment approaches and, an unmet need for therapeutic options that minimize the risk of surgery-related morbidities while simultaneously

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Zuo, Bingguang, Lei Zhang, Zhipei Wang, Li Diao, and Sukun Han. "The Effect and Mechanism of Continuous Passive Exercise Combined with Platelet-Rich Plasma on Rabbit Knee Osteoarthritis Model." Journal of Biomaterials and Tissue Engineering 9, no. 7 (2019): 992–97. http://dx.doi.org/10.1166/jbt.2019.2071.

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Osteoarthritis (OA) is a disease in orthopedics. Continuous passive exercise (CPM) and intraarticular injection of platelet-rich plasma (PRP) have certain effects on OA. However, the effect of these two combination on OA remains unclear. The present study aims to evaluate the treatment efficacy of this combination on OA. 50 New Zealand white rabbits were randomly divided into control group, OA group, CPM group, PRP group and CPM + PRP group followed by analysis of the pathological changes of chondrocyte by H&E staining and Mankin's score, level of IL-1 and TNF-α by ELISA, as well as MMP-1

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Rocha, Flaviana Soares, Lara Maria Alencar Ramos, Jonas Dantas Batista, Darceny Zanetta-Barbosa, Eloísa Amália Vieira Ferro, and Paula Dechichi. "Bovine Anorganic Bone Graft Associated with Platelet-Rich Plasma: Histologic Analysis in Rabbit Calvaria." Journal of Oral Implantology 37, no. 5 (2011): 511–18. http://dx.doi.org/10.1563/aaid-joi-d-09-00091.1.

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Autogenous bone tissue has regeneration potential; however, this capacity may not be sufficient in larger bone defects. The aim of this study is to histologically evaluate anorganic bovine bone grafts (GenOx Inorg) with or without platelet-rich plasma (PRP). Two bone lesions were created in calvaria of 12 rabbits. The 24 surgical lesions were separated into 3 groups: coagulous, anorganic, and anorganic with PRP. At the 4-week time point, the animals were euthanized and the grafted area removed, fixed in formalin 10% with phosphate buffered saline, 0.1 M, and embedded in paraffin. The histologi

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Nakama, Gilberto Y., Sabrina Gonzalez, Polina Matre, et al. "Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study." International Journal of Molecular Sciences 21, no. 19 (2020): 7374. http://dx.doi.org/10.3390/ijms21197374.

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Recent efforts have focused on customizing orthobiologics, such as platelet-rich plasma (PRP) and bone marrow concentrate (BMC), to improve tissue repair. We hypothesized that oral losartan (a TGF-β1 blocker with anti-fibrotic properties) could decrease TGF-β1 levels in leukocyte-poor PRP (LP-PRP) and fibrocytes in BMC. Ten rabbits were randomized into two groups (N = 5/group): osteochondral defect + microfracture (control, group 1) and osteochondral defect + microfracture + losartan (losartan, group 2). For group 2, a dose of 10mg/kg/day of losartan was administrated orally for 12 weeks post-

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Syam, Syamsiah, Chun-Wei Chang, Wen-Chien Lan, et al. "An Innovative Bioceramic Bone Graft with Platelet-Rich Plasma for Rapid Bone Healing and Regeneration in a Rabbit Model." Applied Sciences 11, no. 11 (2021): 5271. http://dx.doi.org/10.3390/app11115271.

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This study aimed to investigate the effect of combining an innovative bioceramic α-calcium sulfate hemihydrate (α-CSH, CaSO4⋅0.5H2O) bone graft and platelet-rich plasma (PRP) to accelerate bone healing and regeneration in a rabbit model. The bone graft material was implanted bilaterally on rabbit’s artificially maxillary sinus defects: the right maxillary sinus received α-CSH, while α-CSH combine with PRP (α-CSH/PRP) was grafted in left site. The quantity and quality of bone formation after implantation were analyzed radiographically and histologically at 1, 2, and 3 weeks. The micro-computed

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Mansouri, Nikta, Hamidreza Fattahian, Kimia Mansouri, Pargol Ghavam Mostafavi, and Abdolmohammad Kajbafzadeh. "The role of fish scale derived scaffold and platelet rich plasma in healing of rabbit tibial defect: an experimental study." Acta Veterinaria Brno 87, no. 4 (2018): 363–70. http://dx.doi.org/10.2754/avb201887040363.

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Fish scale is rich in collagen type I and hydroxyapatite, resembling bone structure. It is readily available, cost effective and can compensate for the limitations of grafting methods such as unavailability, zoonotic disease transmission, and high cost. The aim of this study was to evaluate in vivo the fish scale potential and the possible synergistic effect of platelet rich plasma (PRP) with this scaffold in bone regeneration. Fifteen male white New Zealand rabbits were randomly divided into six groups, each involving 5 limbs. Full thickness bicortical defects were created in the proximal tib

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Nakornnoi, Theerasak, Chidchanok Leethanakul, and Bancha Samruajbenjakun. "Effects of Leukocyte-Platelet-Rich Plasma on the Alveolar Bone Changes During Orthodontic Tooth Movement in Rabbits: A Micro-CT Study." Journal of Indian Orthodontic Society 53, no. 4 (2019): 264–71. http://dx.doi.org/10.1177/0301574219872608.

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Objective: To investigate the effects of leukocyte-platelet-rich plasma (L-PRP) on the alveolar bone changes at the compression and tension sides during orthodontic tooth movement. Materials and Methods: Around 20 New Zealand white rabbits were used in a split-mouth design. The maxillary first premolar was moved mesially with a nickel-titanium closed-coil spring. One side of the maxilla was randomly injected with L-PRP, while the contralateral side served as the control which received normal saline. The alveolar bone adjacent to the maxillary first premolar was scanned using microcomputed tomo

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Filgueiras, R. R., R. J. Del Carlo, N. P. Alves, et al. "Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 66, no. 1 (2014): 168–76. http://dx.doi.org/10.1590/s0102-09352014000100024.

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Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG) received no treatment. The thawed (TH) and fresh (FH) heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP). The CG group showed greatest pain and lameness compared to t

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Nagata, Maria J. H., Michel R. Messora, Flávia A. C. Furlaneto, et al. "Effectiveness of Two Methods for Preparation of Autologous Platelet-Rich Plasma: An Experimental Study in Rabbits." European Journal of Dentistry 04, no. 04 (2010): 395–402. http://dx.doi.org/10.1055/s-0039-1697859.

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Objectives: The purpose of this study was to compare the quantity and quality of platelets in platelet- rich plasma (PRP) samples prepared using either the single- or the double-centrifugation protocol.Methods: Ten adult white New Zealand rabbits were used. Ten ml of blood were drawn from each animal via cardiac puncture. Each blood sample was divided into two equal parts for PRP preparation: 5 ml of blood were centrifuged according to a single-centrifugation protocol (Group I), and 5 ml were centrifuged according to a double-centrifugation protocol (Group II). Manual platelet counts were perf

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Lock, Edward A., Theresa J. Cross, and Rick G. Schnellmann. "Studies on the Mechanism of 4-Aminophenol-induced Toxicity to Renal Proximal Tubules." Human & Experimental Toxicology 12, no. 5 (1993): 383–88. http://dx.doi.org/10.1177/096032719301200507.

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4-Aminophenol (PAP) is known to cause nephrotoxicity in the rat where it produces selective necrosis to renal proximal tubules. The aim of this work was to investigate the toxicity of PAP and its known nephrotoxic metabolite 4-amino-3-S-glutathionylphenol using a well defined suspension of rabbit renal proximal tubules. PAP at a concentration of 0.5 mM and 1 mM caused proximal tubule cell death (measured by lactate dehydrogenase release) in a time-dependent manner over a 4-h exposure. In contrast, 4-amino-3-S-glutathionylphenol at 1 mM produced no proximal tubule cell death over a similar 4-h

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Ihnatouski, Mikhail, Jolanta Pauk, Boris Karev, and Dmitrij Karev. "Nanomechanical Properties of Articular Cartilage Due to the PRP Injection in Experimental Osteoarthritis in Rabbits." Molecules 25, no. 16 (2020): 3734. http://dx.doi.org/10.3390/molecules25163734.

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The purpose of this study was twofold. Firstly, we proposed a measurement protocol for the atomic force microscopy (AFM) method to determine the nanomechanical properties of articular cartilage in experimental osteoarthritis in rabbits. Then, we verified if mechanical properties can be evaluated with AFM shortly after platelet-rich plasma (PRP) injection. We hypothesized that the modulus determined by AFM indentation experiments could be utilized as a progressive disease marker during the treatment of osteoarthritis. The rabbits were equally divided into three groups of six: control (group 1);

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Manjula, B. N., A. S. Acharya, T. Fairwell, and V. A. Fischetti. "Antigenic domains of the streptococcal Pep M5 protein. Localization of epitopes crossreactive with type 6 M protein and identification of a hypervariable region of the M molecule." Journal of Experimental Medicine 163, no. 1 (1986): 129–38. http://dx.doi.org/10.1084/jem.163.1.129.

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Pep M5, the pepsin-derived N-terminal half of the group A streptococcal type 5 M protein exhibits immunologic crossreaction with type 6 M protein, localizing some of the M6-crossreactive epitope(s) within this segment of the M5 protein. Based on the amino acid sequence of the Pep M5 protein, two structurally distinct domains have been recognized within its coiled-coil structure. We have now found that peptides derived from both the structurally distinct domains of the Pep M5 protein contain antigenic epitopes. Furthermore, only the peptides from the C-terminal domain of the Pep M5 protein cros

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Santos, Eliane Szücs dos, Cecília Braga Laposy, Karina Gomes Barros Abegão, et al. "Assessment of the healing of standardized wounds in rabbits treated serially with autologous platelet-rich plasma gel." Semina: Ciências Agrárias 37, no. 6 (2016): 4131. http://dx.doi.org/10.5433/1679-0359.2016v37n6p4131.

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Recent studies have been carried out to evaluate the role of platelet-rich plasma (PRP) in the cicatrization of wounds; however, the protocols for treatment have been based on a single application of PRP.To evaluate the effect of autologous platelet-rich plasma in gel form on the cicatrization of cutaneous wounds in vivo experimental model, wounds were induced in the dorsal areas of six New Zealand white rabbits with the aid of an 8-mm punch. The right side was used as a control (A) and treated with 0.9% NaCl, whereas the left side (B) was treated serially with the autologous platelet-rich pla

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Mack, Suzanne E., and Frederick B. St C. Palmer. "Soluble and membrane-bound polyphosphoinositide phosphohydrolases in mammalian erythrocytes." Biochemistry and Cell Biology 66, no. 3 (1988): 199–207. http://dx.doi.org/10.1139/o88-027.

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Phosphatases and phosphodiesterases that hydrolyse polyphosphoinositides are described in both membrane and cytosol fractions of human, pig, rat, rabbit, and sheep erythrocytes using exogenous substrates. With suitably optimized assay conditions, Ca2+-dependent phosphatidylinositol bisphosphate (PIP2) phosphodiesterase activity was found in the hemoglobin-free cytosol fraction, as well as the membrane. Membrane activity is completely dependent upon Triton X-100 and salt and inhibited by cetyltrimethylammonium bromide (CTAB), while the soluble activity requires CTAB and is inhibited by Triton.

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Candan, Huseyin, Rahsan Yılmaz, Irfan Eser, Umit Aydoğmus, and Samil Gunay. "The Efficacy of Platelet-Rich Plasma in the Treatment of Rib Fractures." Thoracic and Cardiovascular Surgeon 65, no. 07 (2016): 546–50. http://dx.doi.org/10.1055/s-0036-1582261.

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Background Rib fracture is the most common result of thoracic traumas. Intrapulmonary shunt, alveolar capillary membrane damage, intra-alveolar hemorrhage, and hypoxia may develop following rib fractures. Therefore, prompt treatment is important. The aim of this experimental study was to analyze the effects of platelet-rich plasma (PRP) on rib fractures to secure a speedier and more efficient treatment method. Materials and Methods The study involved 18 New Zealand white rabbits, randomly divided into three groups as Group 1, the sham group with no surgical intervention; Group 2, the control g

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Wang, Ketao, Ji Li, Zhongli Li, et al. "Chondrogenic Progenitor Cells Exhibit Superiority Over Mesenchymal Stem Cells and Chondrocytes in Platelet-Rich Plasma Scaffold-Based Cartilage Regeneration." American Journal of Sports Medicine 47, no. 9 (2019): 2200–2215. http://dx.doi.org/10.1177/0363546519854219.

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Background: Platelet-rich plasma (PRP) has been considered a promising tool for cartilage regeneration. However, increasing evidence has demonstrated the controversial effects of PRP on tissue regeneration, partially due to the unsatisfactory cell source. Chondrogenic progenitor cells (CPCs) have gained increasing attention as a potential cell source due to their self-renewal and multipotency, especially toward the chondrogenic lineage, and, thus, may be an appropriate alternative for cartilage engineering. Purpose: To compare the effects of PRP on CPC, mesenchymal stem cell (MSC), and chondro

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Rocha, Flaviana Soares, Jonas Dantas Batista, Darceny Zanetta-Barbosa, and Paula Dechichi. "Effect of Different Storage Media on the Regenerative Potential of Autogenous Bone Grafts: A Histomorphometrical Analysis in Rabbits." Journal of Oral Implantology 39, no. 6 (2013): 635–42. http://dx.doi.org/10.1563/aaid-joi-d-11-00020.

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The success of autogenous bone graft is related to the graft cell viability. In bone-grafting procedures, harvested grafts are often maintained in extraoral media while the recipient site is prepared. The aim of this study was to evaluate in vivo the effect of storage media over autogenous bone grafts during the transsurgical time. Two grafts were removed bilaterally from the calvaria of 18 rabbits. One graft was immediately fixed in the mandibular angle (control group), and the other was maintained in air exposure (dry group), 0.9% NaCl solution (saline group), or platelet-poor plasma (PPP gr

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Varian, Kenneth D., Ying Xu, Carlos A. A. Torres, Michelle M. Monasky, and Paul M. L. Janssen. "A random cycle length approach for assessment of myocardial contraction in isolated rabbit myocardium." American Journal of Physiology-Heart and Circulatory Physiology 297, no. 5 (2009): H1940—H1948. http://dx.doi.org/10.1152/ajpheart.01289.2008.

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It is well known that the strength of cardiac contraction is dependent on the cycle length, evidenced by the force-frequency relationship (FFR) and the existence of postrest potentiation (PRP). Because the contractile strength of the steady-state FFR and force-interval relationship involve instant intrinsic responses to cycle length as well as slower acting components such as posttranslational modification-based mechanisms, it remains unclear how cycle length intrinsically affects cardiac contraction and relaxation. To dissect the impact of cycle length changes from slower acting signaling com

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Kesyan, G. A., G. N. Berchenko, T. G. Nakhapetyan, et al. "Experimental Morphologic and Clinical Substantiation of Autothrombocytic Growth Factors in Complex Treatment of Achilles Tendon Rupture." N.N. Priorov Journal of Traumatology and Orthopedics 19, no. 4 (2012): 32–37. http://dx.doi.org/10.17816/vto20120432-37.

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Study of the influence of autothrombocytic growth factors upon the injured tendinous tissue was performed. Experimental results (60 Chinchilla rabbits, 3—5 kg) showed that administration of autologous platelet-rich plasma (PRP) into Achilles tendon (AT) injury zone promoted activiza- tion of reparative processes with diminution of inflammatory manifestations; increase in angio- genesis, proliferative and synthetic fibroblasts activity, fibrillogenesis processes; earlier tendon healing. Clinical study included 57 patients (33—68years) with acute (8—48 hours) and old (1—12 months) AT ruptures. A

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Joo, Min Wook, Seung Jae Chung, Seung Han Shin, and Yang-Guk Chung. "The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules." Cells Tissues Organs 203, no. 6 (2017): 327–38. http://dx.doi.org/10.1159/000454915.

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To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous gr

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Koch, Matthias, Selma Hammer, Julian Fuellerer, et al. "Bone Marrow Aspirate Concentrate for the Treatment of Avascular Meniscus Tears in a One-Step Procedure—Evaluation of an In Vivo Model." International Journal of Molecular Sciences 20, no. 5 (2019): 1120. http://dx.doi.org/10.3390/ijms20051120.

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Avascular meniscus tears show poor intrinsic regenerative potential. Thus, lesions within this area predispose the patient to developing knee osteoarthritis. Current research focuses on regenerative approaches using growth factors or mesenchymal stem cells (MSCs) to enhance healing capacity within the avascular meniscus zone. The use of MSCs especially as progenitor cells and a source of growth factors has shown promising results. However, present studies use bone-marrow-derived BMSCs in a two-step procedure, which is limiting the transfer in clinical praxis. So, the aim of this study was to e

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Perrot, Jean. "Keep the thread up! From humour to poetry. Silence as a spur to read and speak." Ondina - Ondine, no. 6 (September 7, 2021): 23–35. http://dx.doi.org/10.26754/ojs_ondina/ond.202164513.

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The purpose of this article is to proceed with a semiotic examination of several wordless picturebooks focussed on the use of a thread, Considering that it is the visual image which first and foremost prompts the meaning in iconotexts, we shall deal with it more particularly through the examination of two French picturebooks: the first one by Robert Scouvart, Histoire d’un fil (The Story of a Thread, Magnard 1990) shows how a single thread can magically delineate different characters introduced in an alluring play on words. The book will offer a distanced staging of the reading process through

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Shomer-Ilan, a., GP Jones, and LG Paleg. "In vitro Thermal and Salt Stability of Pyruvate Kinase Are Increased by Proline Analogues and Trigonelline." Functional Plant Biology 18, no. 3 (1991): 279. http://dx.doi.org/10.1071/pp9910279.

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The nitrogenous compounds N-methyl-L-proline (MP), trans-4-hydroxy-N-methyl-L-proline (MHP) and trigonelline (T), which undergo stress-induced accumulation in some Australian plants, were analysed and compared with proline (P) and glycinebetaine (B) for possible protective roles. The activity of pyruvate kinase (PK), prepared from Zea mays leaves and rabbit muscle, was unaffected even in the presence of 750 mM of the proline analogues. Thus, MP and MHP, like P and B, have the properties to act in vivo as compatible osmotica. T was not as compatible, decreasing enzyme activity 20% at 0.5 M. Lik

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Oliveira Filho, Marco Antonio de, Paulo Afonso Nunes Nassif, Osvaldo Malafaia, et al. "Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits." Acta Cirurgica Brasileira 25, no. 1 (2010): 28–33. http://dx.doi.org/10.1590/s0102-86502010000100008.

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PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th a

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Turcotte, A., M. Simard, N. J. Morin, D. Beauchamp, and M. G. Bergeron. "Differential distributions in tissues and efficacies of aztreonam and ceftazidime and in vivo bacterial morphological changes following treatment." Antimicrobial Agents and Chemotherapy 41, no. 2 (1997): 401–9. http://dx.doi.org/10.1128/aac.41.2.401.

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The differential tissue distributions of aztreonam and ceftazidime within fibrin clots infected with Pseudomonas aeruginosa, Enterobacter cloacae, and Serratia marcescens, their efficacies, and the in vivo bacterial morphological changes induced by these drugs were evaluated. Rabbits were given intravenously a single dose of 100 mg of either agents/kg of body weight. In the cores of the clots, the peak levels of both drugs were much lower than those observed in the peripheries and in serum. Aztreonam's half-lives within the peripheries and in the cores of the fibrin clots were up to six times

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Garcez, Tuane Nerissa Alves, Helena Flores Mello, Priscilla Domingues Mörschbacher, et al. "Liquid and Gel Platelet Rich Plasma as Skin Healing Adjuvant." Acta Scientiae Veterinariae 44, no. 1 (2018): 7. http://dx.doi.org/10.22456/1679-9216.80931.

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Background: In recent decades, many researches have been conducted on processes involved in tissue repairing, mainly in the development of resources and technology designed to improve the wound healing progress. Platelet rich plasma (PRP) derived from autologous blood is defined as a plasma volume with platelet concentration higher than physiological level. It is an autogenous and low cost source of growth factors, which are essential for tissue regeneration due to their angiogenic, mitogenic, and chemotactic properties. The aim of this study was evaluate two forms of PRP- liquid and gel - reg

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Shao, Zhengwei, Chengqi Lyu, Lin Teng, et al. "An Injectable Fibrin Scaffold Rich in Growth Factors for Skin Repair." BioMed Research International 2021 (February 8, 2021): 1–13. http://dx.doi.org/10.1155/2021/8094932.

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Platelet aggregates, such as PRP, PRF, and CGF, have been used alone or in combination with other grafting materials to enhance restoration outcomes. The process for preparing these autografting materials requires two-step centrifugation or specific centrifuges. In this study, we obtained an injectable fibrin scaffold (IFS) rich in growth factors by one-step centrifugation of whole blood from rabbits. The purpose of this study is to introduce some characteristics of IFS. This scaffold was characterized using various techniques, including Masson’s trichrome staining, scanning electron microscop

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Johnson, M. D., M. E. Gray, R. B. Pepinsky, and M. T. Stahlman. "Lipocortin-1 immunoreactivity in the human pituitary gland." Journal of Histochemistry & Cytochemistry 38, no. 12 (1990): 1841–45. http://dx.doi.org/10.1177/38.12.2147709.

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We evaluated the distribution of lipocortin-1 immunoreactivity in 118 immature or mature human hypophyses using the peroxidase-antiperoxidase (PAP) technique with a polyclonal rabbit antiserum against lipocortin-1. Serial sections were evaluated for five pituitary hormones and S-100 protein immunoreactivity to compare their distributions with that of lipocortin-1. Scattered or moderate numbers of cells exhibited lipocortin-1 immunoreactivity in the pars distalis of 89 subjects ranging in age from 27 weeks' gestation to 83 years. Seven immature and seven aged specimens exhibited no immunostaini

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Ozhegov, Evgeny, Ramprasad Srinivasan, and Vladimir Bogdanov. "Alternatively Spliced Tissue Factor (asTF) Is Elevated in the Plasma of Patients with Sickle Cell Disease: Pilot Studies Performed Using a Novel asTF-Specific ELISA." Blood 118, no. 21 (2011): 2240. http://dx.doi.org/10.1182/blood.v118.21.2240.2240.

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Abstract Abstract 2240 Background and Rationale: Vasoocclusive crises are a major hallmark of sickle cell disease (SCD) pathobiology; experimental evidence suggests that SCD vasoocclusion can be triggered by the increased adhesion of white blood cells, including monocytes, to the microvascular endothelium. Pro-coagulant activity of Tissue Factor, the trigger of blood coagulation, is heightened in the blood of patients with SCD. We recently reported that, compared to full length Tissue Factor (flTF), alternatively spliced Tissue Factor (asTF) acts as a very potent inducer of cell adhesion molec

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Utomo, Dwikora Novembri, and Kukuh Dwiputra Hernugrahanto. "THE ROLE OF PLATELET-RICH FIBRIN FACILITATES THE HEALING OF GASTROCNEMIUS MUSCLE DEFECT: A PRELIMINARY STUDY ON ANIMAL MODEL." (JOINTS) Journal Orthopaedi and Traumatology Surabaya 7, no. 1 (2019): 31. http://dx.doi.org/10.20473/joints.v7i1.2018.31-41.

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Background: Scar formation has been a classical issue in the healing of skeletal muscle defect which will results in decrease of strength and function. Platelet-rich plasma (PRP) has been studied to enhance the healing of muscle defect. The disadvantages of it include the risk of immunologic reaction, time-consuming preparation, and lack of scaffold element. Platelet-rich fibrin (PRF) is a new generation of platelet concentrate that provides good source of growth factors and scaffold element necessary for the healing. This study is to provide a fondation of PRF preparation and implantation for

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Gajewski, J. L., J. McMannis, E. Shpall, et al. "A pilot study CD34 selected haploidentical transplant using a chemotherapy-only preparative regimen and intensive antibiotic prophylaxis for treatment of advanced leukemia." Journal of Clinical Oncology 25, no. 18_suppl (2007): 7097. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.7097.

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7097 Background: We present preliminary data of haploidentical transplant for 13 patients (pts) with acute leukemia. Methods: Phase II Study Results: We present preliminary data of haploidentical transplant for 13 patients (pts) with acute leukemia using a chemotherapy-only preparative regimen of Melphalan 140 mg/m2 on day −8; Thiotepa 10 mg/kg on day −7; Fludarabine 40 mg/m2 on days −6, −5, −4, −3; 1.5 mg/kg of rabbit ATG/day days −6, −5, −4, −3. The transplant was administered on day 0 using positive selection of CD34 positive cells by the CliniMACS device as a means of T-cell depletion. All

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