Relevant bibliographies by topics / Whole Cell Voltage Clamp

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Whole Cell Voltage Clamp'

Author: Grafiati
Published: 4 June 2021
Last updated: 18 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Whole Cell Voltage Clamp.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Whole Cell Voltage Clamp"

1

Jackson, Meyer B. "Whole-Cell Voltage Clamp Recording." Current Protocols in Neuroscience 00, no. 1 (September 1997): 6.6.1–6.6.30. http://dx.doi.org/10.1002/0471142301.ns0606s00.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Grygorczyk, Ryszard, and Michael A. Bridges. "Whole-cell chloride conductances in cultured brushed human nasal epithelial cells." Canadian Journal of Physiology and Pharmacology 70, no. 8 (August 1, 1992): 1134–41. http://dx.doi.org/10.1139/y92-157.

Full text
Abstract:
Human airway epithelial cells were obtained by nasal brushing, thus avoiding the use of proteolytic enzymes for cell isolation. Whole-cell Cl− conductances were studied in these cells by means of the patch-clamp technique. During whole-cell recordings, cell swelling activated a Cl− conductance that was blocked by indanyloxyacetic acid (48 ± 10% inhibition at 50 μM). The swelling-induced current outwardly rectified and showed inactivation at depolarizing voltages (≥ +60 mV) and activation at hyperpolarizing voltages (≤ −30 mV). The voltage sensitivity of current activation was approximately twice that of inactivation. Another Cl− current with different kinetics was observed when nonswollen airway cells were stimulated with ionomycin (2 μM) in the presence of 1 mM Ca2+. The Ca2+-induced current exhibited activation during depolarizing voltage steps (≥ +40 mV) and inactivation during hyperpolarizing voltage steps (≤ −40 mV). In contrast to the swelling-induced current, the activation of Ca2+-induced current was less sensitive to voltage compared with its inactivation. Tail current analysis suggested that Cl− channels having a linear current–voltage relation mediate the response to Ca2+. This study indicates that brushed human nasal epithelial cells possess Cl− conductances that are regulated by cell swelling and Ca2+ and that they represent a useful in vitro model for studying ion transport in epithelia.Key words: chloride channels, epithelia, airway, patch clamp.
APA, Harvard, Vancouver, ISO, and other styles
3

Harrison, Reid R., Ilya Kolb, Suhasa B. Kodandaramaiah, Alexander A. Chubykin, Aimei Yang, Mark F. Bear, Edward S. Boyden, and Craig R. Forest. "Microchip amplifier for in vitro, in vivo, and automated whole cell patch-clamp recording." Journal of Neurophysiology 113, no. 4 (February 15, 2015): 1275–82. http://dx.doi.org/10.1152/jn.00629.2014.

Full text
Abstract:
Patch clamping is a gold-standard electrophysiology technique that has the temporal resolution and signal-to-noise ratio capable of reporting single ion channel currents, as well as electrical activity of excitable single cells. Despite its usefulness and decades of development, the amplifiers required for patch clamping are expensive and bulky. This has limited the scalability and throughput of patch clamping for single-ion channel and single-cell analyses. In this work, we have developed a custom patch-clamp amplifier microchip that can be fabricated using standard commercial silicon processes capable of performing both voltage- and current-clamp measurements. A key innovation is the use of nonlinear feedback elements in the voltage-clamp amplifier circuit to convert measured currents into logarithmically encoded voltages, thereby eliminating the need for large high-valued resistors, a factor that has limited previous attempts at integration. Benchtop characterization of the chip shows low levels of current noise [1.1 pA root mean square (rms) over 5 kHz] during voltage-clamp measurements and low levels of voltage noise (8.2 μV rms over 10 kHz) during current-clamp measurements. We demonstrate the ability of the chip to perform both current- and voltage-clamp measurement in vitro in HEK293FT cells and cultured neurons. We also demonstrate its ability to perform in vivo recordings as part of a robotic patch-clamping system. The performance of the patch-clamp amplifier microchip compares favorably with much larger commercial instrumentation, enabling benchtop commoditization, miniaturization, and scalable patch-clamp instrumentation.
APA, Harvard, Vancouver, ISO, and other styles
4

Shkodrov, Georgi B. "Computer program for performing whole-cell voltage-clamp experiments." Computer Methods and Programs in Biomedicine 48, no. 3 (December 1995): 241–46. http://dx.doi.org/10.1016/0169-2607(95)01696-1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Wolfe, Joshua T., Bryan A. Krantz, G. Jonah A. Rainey, John A. T. Young, and R. John Collier. "Whole-cell Voltage Clamp Measurements of Anthrax Toxin Pore Current." Journal of Biological Chemistry 280, no. 47 (September 23, 2005): 39417–22. http://dx.doi.org/10.1074/jbc.m509049200.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Nasi, E. "Whole-cell clamp of dissociated photoreceptors from the eye of Lima scabra." Journal of General Physiology 97, no. 1 (January 1, 1991): 35–54. http://dx.doi.org/10.1085/jgp.97.1.35.

Full text
Abstract:
Voltage-dependent membrane currents were investigated in enzymatically dissociated photoreceptors of Lima scabra using the whole-cell clamp technique. Depolarizing steps to voltages more positive than -10 mV elicit a transient inward current followed by a delayed, sustained outward current. The outward current is insensitive to replacement of a large fraction of extracellular Cl- with the impermeant anion glucuronate. Superfusion with tetraethylammonium and 4-aminopyridine reversibly abolishes the outward current, and internal perfusion with cesium also suppresses it, indicating that it is mediated by potassium channels. Isolation of the inward current reveals a fast activation kinetics, the peak amplitude occurring as early as 4-5 ms after stimulus onset, and a relatively rapid, though incomplete inactivation. Within the range of voltages examined, spanning up to +90 mV, reversal was not observed. The inward current is not sensitive to tetrodotoxin at concentrations up to 10 microM, and survives replacement of extracellular Na with tetramethylammonium. On the other hand, it is completely eliminated by calcium removal from the perfusing solution, and it is partially blocked by submillimolar concentrations of cadmium, suggesting that it is entirely due to voltage-dependent calcium channels. Analysis of the kinetics and voltage dependence of the isolated calcium current indicates the presence of two components, possibly reflecting the existence of separate populations of channels. Barium and strontium can pass through these channels, though less easily than calcium. Both the activation and the inactivation become significantly more sluggish when these ions serve as the charge carrier. A large fraction of the outward current is activated by preceding calcium influx. Suppression of this calcium-dependent potassium current shows a small residual component resembling the delayed rectifier. In addition, a transient outward current sensitive to 4-aminopyridine (Ia) could also be identified. The relevance of such conductance mechanisms in the generation of the light response in Lima photoreceptors is discussed.
APA, Harvard, Vancouver, ISO, and other styles
7

Mleux, Benoit Saint, and L. E. Moore. "Active Dendritic Membrane Properties of XenopusLarval Spinal Neurons Analyzed With a Whole Cell Soma Voltage Clamp." Journal of Neurophysiology 83, no. 3 (March 1, 2000): 1381–93. http://dx.doi.org/10.1152/jn.2000.83.3.1381.

Full text
Abstract:
Voltage- and current-clamp measurements of inwardly directed currents were made from the somatic regions of Xenopus laevisspinal neurons. Current-voltage ( I-V) curves determined under voltage clamp, but not current clamp, were able to indicate a negative slope conductance in neurons that showed strong accommodating action potential responses to a constant current stimulation. Voltage-clamp I-V curves from repetitive firing neurons did not have a net negative slope conductance and had identical I-V plots under current clamp. Frequency domain responses indicate negative slope conductances with different properties with or without tetrodotoxin, suggesting that both sodium and calcium currents are present in these spinal neurons. The currents obtained from a voltage clamp of the somatic region were analyzed in terms of spatially controlled soma membrane currents and additional currents from dendritic potential responses. Linearized frequency domain analysis in combination with both voltage- and current-clamp responses over a range of membrane potentials was essential for an accurate determination of consistent neuronal model behavior. In essence, the data obtained at resting or hyperpolarized membrane potentials in the frequency domain were used to determine the electrotonic structure, while both the frequency and time domain data at depolarized potentials were required to characterize the voltage-dependent channels. Finally, the dendritic and somatic membrane properties were used to reconstruct the action potential behavior and quantitatively predict the dependence of neuronal firing properties on electrotonic structure. The reconstructed action potentials reproduced the behavior of two broad distributions of interneurons characterized by their degree of accommodation. These studies suggest that in addition to the ionic conductances, electrotonic structure is correlated with the action potential behavior of larval neurons.
APA, Harvard, Vancouver, ISO, and other styles
8

Bolton, T. B., R. J. Lang, T. Takewaki, and C. D. Benham. "Patch and Whole-Cell Voltage-Clamp Studies on Single Smooth Muscle Cells." Journal of Cardiovascular Pharmacology 8 (1986): S20—S24. http://dx.doi.org/10.1097/00005344-198600088-00005.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Velte, T. J., and R. F. Miller. "Computer simulations of voltage clamping retinal ganglion cells through whole-cell electrodes in the soma." Journal of Neurophysiology 75, no. 5 (May 1, 1996): 2129–43. http://dx.doi.org/10.1152/jn.1996.75.5.2129.

Full text
Abstract:
1. Computer simulations of voltage-clamp experiments in retinal ganglion cells were implemented to better understand the insights that can be obtained with this physiological approach. 2. Simulation studies of voltage clamping were based on the contemporary approach of using whole-cell recordings with low resistance electrodes attached to the soma. Realistic ganglion cell morphologies were provided by cell staining experiments in the mudpuppy retina; selected cells included small-, medium-, and large-field neurons whose morphologies were entered into a computer through a neuron tracing program. 3. Values for the specific membrane resistance (Rm) varied from 5,000 to 100,000 omega/cm2 to conform to the range of Rm values obtained with intracellular sharp electrodes and whole-cell recordings. 4. Synaptic input currents were simulated by injecting current with and without an underlying conductance change into different regions of the dendritic tree. The time-variant waveform of the current included a combined transient and sustained component similar to the waveform of ON-bipolar activation. 5. Simulations were base on 1) intact structures, which included the soma and the entire dendritic tree, and 2) a more limited cell geometry that included representation of the soma, but only part of the dendritic tree, to represent the restricted morphology that might be rendered after cutting the retina into 150-microns cross sections for retinal slice experiments. 6. The results of this study indicate that voltage clamping from the soma, with optimal, low resistance electrodes and series resistance compensation, provides an error-free voltage clamp for slow signals that are generated within a small electrotonic distance from the soma (approximately 0.1 lambda). 7. The ideal voltage-clamp conditions are optimized when synaptic conductances are small and nonlinear membrane elements are minimally activated: small-field neurons best approximate these conditions, but clamping errors are evident in these cells when more distal branches are activated. The degree of error in voltage clamping was much greater when medium-and large-field neurons were evaluated. 8. It was not possible to clamp action potentials (nonpropagating) even when they were generated near the soma in any of the three model cells examined. 9. Experimental paradigms were developed to demonstrate that inadequate voltage clamping can lead to errors in the interpretation of experimental data when relevant variables are not taken into consideration. Suggestions are made for determining and optimizing favorable clamp conditions.
APA, Harvard, Vancouver, ISO, and other styles
10

Budai, Dénes, Lois J. Kehl, Georgetta I. Poliac, and George L. Wilcox. "An iconographic program for computer-controlled whole-cell voltage clamp experiments." Journal of Neuroscience Methods 48, no. 1-2 (June 1993): 65–74. http://dx.doi.org/10.1016/s0165-0270(05)80008-1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Whole Cell Voltage Clamp"

1

Wang, Junjie. "Functional and Structural Study of Pannexin1 Channels." Scholarly Repository, 2009. http://scholarlyrepository.miami.edu/oa_dissertations/207.

Full text
Abstract:
Pannexins are vertebrate proteins with limited sequence homology to the invertebrate gap junction proteins, the innexins. However, in contrast to innexins and the vertebrate connexins, pannexins do not form gap junction channels. Instead they appear to solely function as unpaired membrane channels allowing the flux of molecules, including ATP, across the plasma membrane. We provided additional evidence for their ATP release function by demonstrating that the connexin mimetic peptides, which were thought to inhibit ATP release through connexin channels, do not inhibit their host connexin channels but instead inhibit pannexin1 channels by a mechanism of steric block. Therefore, the inhibitory effects of mimetic peptides on ATP release may represent supporting evidence for a role of pannexin1 in ATP release. We also analyzed the pore structure of pannexin1 channels with the Substituted Cysteine Accessibility Method. The thiol reagents MBB and MTSET reacted with several positions in the external portion of the first transmembrane segment and the first extracellular loop. In addition, MTSET reactivity was found in the internal portion of TM3. These data suggest that portions of TM1, E1 and TM3 line the pore of pannexin1 channels. Thus, the pore structure of pannexin1 is similar to that of connexin channels.
APA, Harvard, Vancouver, ISO, and other styles
2

Kodandaramaiah, Suhasa Bangalore. "Robotics for in vivo whole cell patch clamping." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/51932.

Full text
Abstract:
Whole-cell patch clamp electrophysiology of neurons in vivo enables the recording of electrical events in cells with great precision, and supports a wide diversity of morphological and molecular analysis experiments important for the understanding of single-cell and network functions in the intact brain. However, high levels of skill are required in order to perform in vivo patching, and the process is time-consuming and painstaking. Robotic systems for in vivo patching would not only empower a great number of neuroscientists to perform such experiments, but would also open up fundamentally new kinds of experiment enabled by the resultant high throughput and scalability. We discovered that in vivo blind whole cell patch clamp electrophysiology could be implemented as a straightforward algorithm and developed an automated robotic system that was capable of performing this algorithm. We validated the performance of the robot in both the cortex and hippocampus of anesthetized mice. The robot achieves yields, cell recording qualities, and operational speeds that are comparable to, or exceed, those of experienced human investigators. Building upon this framework, we developed a multichannel version of “autopatcher” robot capable establishing whole cell patch clamp recordings from pairs and triplets of neurons in the cortex simultaneously. These algorithms can be generalized to control arbitrarily large number of electrodes and the high yield, throughput and automation of complex set of tasks results in a practical solution for conducting patch clamp recordings in potentially dozens of interconnected neurons in vivo.
APA, Harvard, Vancouver, ISO, and other styles
3

Mansell, Steven A. "The characterisation of ion channels in human spermatozoa by whole cell patch clamp electrophysiology." Thesis, University of Dundee, 2013. https://discovery.dundee.ac.uk/en/studentTheses/5fc0b4d5-ac64-474d-9cf3-5a123fa665cb.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Zhou, Yun. "STUDY OF SINGLE CELL SONOPORATION IN REAL TIME USING ELECTROPHYSIOLOGY TECHNIQUES." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1207251863.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Zheng, Tianyang [Verfasser]. "Novel chip based microelectrode cavity arrays for whole-cell planar patch-clamp recording / Tianyang Zheng." München : Verlag Dr. Hut, 2016. http://d-nb.info/1103872516/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Nightingale, Wendy Denise. "Electrical characteristics if an identified insect motoneurone cell body : a current- and voltage-clamp." Thesis, University of St Andrews, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329911.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Nightingale, Wendy Denise. "Electrical characteristics of an identified insect motoneurone cell body : a current- and voltage-clamp study." Thesis, University of St Andrews, 1989. http://hdl.handle.net/10023/14077.

Full text
Abstract:
1. The electrical characteristics of the cell body of an identified excitatory motoneurone (cell 3) from the cockroach (Periplaneta americana) have been studied under current- and voltage-clamp. 2. Under voltage-clamp depolarising command pulses evoked an outward current which increased with the magnitude of the command step up to approximately +100mV, a component of the current developed more slowly and took longer to reach a maximum. With increasing depolarisation the outward current response fell to a lower level before further increasing. This current response gave rise to a characteristic N-shape I-V relationship. The position of the negative conductance region depends on the time current measurements are taken after the onset of the command pulse. 3. Externally applied cadmium (1mM) or manganese ions (5mM) abolished the slowly developing current responsible for the hump in the I-V relationship. These results indicate that calcium ions are required for the activation of this component of the outward current. Verapamil (50?M) also reduced this current component and appeared to be non-specific in reducing another current component. Furthermore, verapamil caused inactivation of the remaining current which was more marked for long duration (500ms) command pulses. 4. Externally applied TEA+ (at concentrations greater than 25mM) blocked the calcium-dependent current and a calcium-independent component. Under current-clamp TEA+ (50mM) unmasked a broad action potential. 5. Externally applied aminopyridines did not enhance excitability under current-clamp. Under voltage-clamp aminopyridines had significant effect in shifting the voltage dependence of the hump in the I-V relationship toward more negative potentials. 6. When holding at -90mV and stepping to more positive potentials there was no indication of an early, fast, transient component similar to IA. If present at all, IA made only a minor contribution to the total outward currents. 7. A double command pulse regime was used to study tail currents whereby a standard pre-pulse (pulse (I)) was immediately followed by a test pulse (pulse (II)) to various command potentials. Tail current measurements were taken during pulse (II). The tail currents showed strong outward rectification and were severely reduced in saline containing cadmium ions (ImM). 8. The tail-current reversal potential was dependent on the pulse (I) magnitude and duration. Preliminary results indicated that increasing the pulse (I) magnitude caused a negative shift in reversal potential. Increasing the pulse (I) duration from 10ms to 50ms caused a positive shift in the reversal potential equivalent to a two-fold increase in extracellular cation concentration. 9. A five-fold increase (from 3.1 to 15mM) in external potassium ion concentration produced a small and variable shift in reversal potential, which did not conform to that predicted by the Nernst equation. A five-fold decrease (from 235 to 47mM) in external chloride ion concentration had little effect on the tail current reversal potential but did cause a slight reduction in the outward currents. Furthermore, the voltage dependency of the hump in the I-V relationship was shifted toward more negative potentials. 10. Action potentials induced by intracellular citrate injection were only slightly enhanced by a four-fold increase (from 9 to 36mM) in external calcium ion concentration. They were reversibly reduced to a graded spike in saline containing verapamil (10?M) and reversibly abolished by manganese ions (40mM), but were relatively unaffected by sodium-free saline. These observations suggest that calcium ions were the major ion carrying the inward current under these conditions. 11. Carbon dioxide-induced action potentials were reversibly reduced to a graded spike in sodium-free or manganese saline (40mM) whereas tetrodotoxin (50nM) irreversibly abolished action potentials for wash period up to 20mins. These observations suggest that both calcium and sodium ions were responsible for the inward current under these conditions. 12. The regenerative component of the axotomy-induced action- potentials was reversibly reduced in sodium-free saline and only partially reduced with some broadening in calcium-free or manganese saline (40mM). Either treatment alone was insufficient to completely abolish or reduce the action potential to a graded spike. A combination of Na-free saline with manganese ions (40mM) caused a more complete block by reducing the regenerative component to a graded spike. These results suggest that sodium ions, and to a lesser extent, calcium ions were responsible for the inward current under these conditions.
APA, Harvard, Vancouver, ISO, and other styles
8

Griguer, Corinne. "Conductances ioniques et processus adaptatifs des cellules sensorielles vestibulaires chez le cobaye : étude en configuration "Whole Cell Clamp"." Montpellier 2, 1992. http://www.theses.fr/1992MON20248.

Full text
Abstract:
L'etude des cellules vestibulaires de type i et ii isolees a partir des canaux semi-circulaires de cobaye en configuration whole cell clamp nous a permis de mettre en evidence et de caracteriser d'une part sur les cellules de type ii, un courant potassique i#k, un courant potassique calcium dependant et des phenomenes de resonance membranaire. Sur les cellules de type i, nous avons caracterise un courant potassique active au repos (i#k#v#i), un courant entrant potassique s'activant lors d'hyperpolarisations membranaires (i#i#r) et un courant majoritairement potassique et faiblement calcique s'inversant entre 60 mv et 40 mv. D'autre part, les cellules de type i presentent des mouvements reversibles affectant leur apex. Ces mouvements ont pu etre induits lors de stimulations chimiques, mecaniques ou electriques. Ces differentes proprietes ioniques et mecaniques suggerent des modalites specifiques du traitement de l'information par ces deux types de cellules
APA, Harvard, Vancouver, ISO, and other styles
9

Grimes, Julia Anne. "A patch clamp study of voltage-gated ion channels in rat prostate cancer cell lines : relation to metastatic ability." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326141.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Chittam, Harish Kumar. "Modulation of Whole Cell Currents in Human Neuroblastoma Cells via the Hormone Aldosterone: An in vitro Study." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6074.

Full text
Abstract:
Ion channels play a critical role in maintaining homeostasis by moving various ions in and out of cells. The Na+-K+-2Cl- or NKCC1 ion channel is involved in the regulation of Na+, K+, and Cl- across cell membranes, and plays a key role in many forms of cellular physiology. In the cochlea, NKCC1 is involved in endolymph production and maintenance of the endocochlear potential. Our hypothesis is that blocking NKCC1 channels should directly impact auditory sensitivity causing hearing loss. Our lab has also shown that the hormone aldosterone (ALD) can upregulate NKCC1 protein expression in vitro and in vivo. In the present investigation, we use electrophysiology and molecular biology techniques to study the biophysical mechanisms underlying the action of ALD in vitro on NKCC1 in the SH-SY5Y cell line. Our initial protein expression studies using RT-PCR found that proteins specific to NKCC1channels were present in SH-SY5Y neuronal cells. Whole cell currents measured using patch clamp methodology, were used to analyze the effects of various compounds on NKCC1 in the SH-SY5Y cell line. Control data were collected under perfusion of extracellular solution (ECS), then ECS containing 10µM bumetanide was applied, and, finally a washout condition completed the experiment. Similar experiments were conducted using ALD, and we observed an increase in K+ currents when bumetanide as well as when ALD was applied. This is the first report that indicates that ALD can directly regulate K+ channels in SH-SY5Y cells.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Whole Cell Voltage Clamp"

1

1931-, Smith T. G., ed. Voltage and patch clamping with microelectrodes. Bethesda, Md: American Physiological Society, 1985.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Graves, Smith Thomas, ed. Voltage and patch clamping with microelectrodes. Bethesda, Md: American Physiological Society, 1985.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Weiner, J. L. Ethanol modulation of GABA[A]-mediated synaptic transmission in hippocampal CA1 neurons: A whole-cell patch-clamp study. 1994.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

(Editor), John N. Abelson, Melvin I. Simon (Editor), Bernardo Rudy (Editor), and Linda E. Iverson (Editor), eds. Ion Channels, Volume 207 (Methods in Enzymology). Academic Press, 1992.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

1953-, Iverson Linda E., Conn P. Michael, and Rudy Bernardo, eds. Ion channels. San Diego: Academic Press, 1992.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Kawamura, Masahito. Ketogenic Diet in a Hippocampal Slice. Edited by Detlev Boison. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190497996.003.0021.

Full text
Abstract:
The hippocampus is thought to be a good experimental model for investigating epileptogenesis in and/or antiepileptic therapy for temporal lobe epilepsy. The hippocampus is also a useful target for researching the ketogenic diet. This chapter focuses on electrophysiological recordings using hippocampal slices and introduces their use for studying the anticonvulsant effects underlying ketogenic diets. The major difficulty in using hippocampal slices is the inability to precisely reproduce the in vivo condition of ketogenic diet feeding in this in vitro preparation. Three different approaches are reported to reproduce diet effects in the hippocampal slices: (1) direct application of ketone bodies, (2) mimicking the ketogenic diet condition with whole-cell patch-clamp technique, and (3) hippocampal slices from ketogenic diet–fed animals. Significant results have been found with each of these methods. These three approaches are useful tools to elucidate the underlying anticonvulsant mechanisms of the ketogenic diet.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Whole Cell Voltage Clamp"

1

López-Barneo, José. "Voltage-Dependent Ionic Channels: “Whole-Cell” Recording by Patch-Clamp Techniques." In Cell Membrane Transport, 215–37. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4757-9601-8_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Lefebvre, Romain, Sandrine Pouvreau, Claude Collet, Bruno Allard, and Vincent Jacquemond. "Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique." In Methods in Molecular Biology, 159–70. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1096-0_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Pouvreau, Sandrine, Claude Collet, Bruno Allard, and Vincent Jacquemond. "Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique." In Methods in Molecular Biology, 185–94. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-529-9_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Brown, Jon, Atticus H. Hainsworth, Alessandro Stefani, and Andrew D. Randall. "Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones." In Methods in Molecular Biology, 123–48. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-086-1_7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Van Hook, Matthew J., and Wallace B. Thoreson. "Whole-Cell Patch-Clamp Recording." In Springer Protocols Handbooks, 353–67. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8794-4_25.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Sontheimer, Harald, and Michelle L. Olsen. "Whole-Cell Patch-Clamp Recordings." In Neuromethods, 35–68. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-492-6_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Mahfooz, Kashif, and Tommas J. Ellender. "Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing." In Patch Clamp Electrophysiology, 179–89. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Kokinovic, Bojana, Stylianos Papaioannou, and Paolo Medini. "In Vivo Whole-Cell Recordings." In Advanced Patch-Clamp Analysis for Neuroscientists, 1–19. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3411-9_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Linley, John E. "Perforated Whole-Cell Patch-Clamp Recording." In Methods in Molecular Biology, 149–57. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-351-0_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Rosholm, Kadla R., Kim Boddum, and Anders Lindquist. "Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology." In Patch Clamp Electrophysiology, 93–108. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0818-0_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Whole Cell Voltage Clamp"

1

Nguyen, Mary-Anne, Nima Tamaddoni, and Stephen A. Sarles. "Interrogation of Bilayers in a Multi-Droplet Cluster for Membrane-Based Sensing." In ASME 2015 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/smasis2015-8970.

Full text
Abstract:
The long-term vision of our work is to create a new class of smart material that utilizes networks of active, synthetic cell membranes for sensing, actuation, and energy harvesting. Having multiple membrane structures is specifically targeted because a higher density of functional membranes is expected to enable amplification and collective utility, similar to how living tissues and organisms utilize networks of highly connected cells to accomplish large tasks. While there are several known methods for assembling droplet-based networks of synthetic lipid bilayers, there has been much less effort to develop methods for electrically characterizing each interface in a multi-bilayer-droplet network. This paper specifically focuses on a strategy for using electrical measurements to independently record transmembrane currents occurring at each bilayer in multi-bilayer networks where the number of bilayers present is equal to or greater than the number of droplets in the system. Using a multichannel patch clamp amplifier, we develop a measurement technique for sequentially assigning sensing electrodes to apply a non-zero voltage or function as virtual ground (V=0). Experimental studies on a three-droplet cluster containing three bilayers confirm the validity of the proposed approach for independently interrogating each membrane, and the results allow extension of the method to networks with 4–7 droplets. Furthermore, alamethicin peptide gating is monitored using the measurement cycle in order to interrogate all interfaces. Due to high total membrane area, highly packed systems can provide an increase in the magnitude of sensing current generated by a stimulus. Such amplification could feasibly be employed in droplet-based hair cell sensing applications in which airflow or vibration acts as the perturbation source, and the proposed approach and challenges for interrogating the transduction response in a multi-membrane hair cell sensor are discussed herein.
APA, Harvard, Vancouver, ISO, and other styles
2

Weerakoon, Pujitha, Kate Klemic, Fred J. Sigworth, and Eugenio Culurciello. "An Integrated Patch-Clamp Amplifier for High-Density Whole-Cell Recordings." In 2007 IEEE International Symposium on Circuits and Systems. IEEE, 2007. http://dx.doi.org/10.1109/iscas.2007.378324.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Boller, Till, Ralph Kennel, and Guenter Schmitt. "Modular regenerative switching cell with integrated voltage-clamp." In 2008 IEEE Power Electronics Specialists Conference - PESC 2008. IEEE, 2008. http://dx.doi.org/10.1109/pesc.2008.4592642.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

EVANS, MICHAEL G., and ROBERT FETTIPLACE. "MEASUREMENT OF OUTER HAIR CELL ELECTROMOTILITY USING A FAST VOLTAGE CLAMP." In Proceedings of the 10th International Workshop on the Mechanics of Hearing. WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812833785_0064.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Zheng, Tianyang, Gerhard Baaken, Jurgen Ruhe, Jan C. Behrend, and Rong Zhu. "Notice of Violation of IEEE Publication Principles: Chip Based Microelectrochemical Cell Array for Whole-Cell Patch-Clamp Recording." In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). IEEE, 2019. http://dx.doi.org/10.1109/transducers.2019.8808576.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Mariam, Hasetetsion G., J. Rick Baer, David J. Scholl, Ronald P. Cooper, Daniel E. Wilkosz, Anthony J. Grima, and Larry V. Reatherford. "Ultrasonic Welding of Aluminum 6111: Reliability and Maintainability Study of Robot Mounted C-Gun Welding System." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-42929.

Full text
Abstract:
Ultrasonic metal welding (USW) is a promising joining method for aluminum automotive body construction applications. During USW, aluminum weldments are joined together by applying high frequency vibrations while holding the parts together with a moderate clamping force. In an effort to further the development of USW for high volume robotic body construction applications, a reliability and maintainability study was performed using a robotic welding cell installed in the Ford Research and Innovation Center. The robot was equipped with a modified Sonobond ultrasonic metal welder, which was mounted on a C-frame. The study consisted of welding fully overlapped 550 mm × 350 mm × 0.9mm thick AA6111-T4 aluminum panels with 330 welds on each panel until 100,000 welds were made. Consistency in welder operation was monitored by welding fully overlapped AA6111-T4 aluminum strips (25mm wide × 550 mm long × 0.9mm thick) at the end of each day’s welding and then tensile testing the strips in a T-peel configuration. There was no statistical difference in average T-peel strength over the course of the 100,000 weld study. There was also no degradation noted in lap shear failure loads between samples welded at the end of the 100,000 weld study and those generated before initiation of the study. Reliability of the USW process during this study was monitored by periodic inspection of the robot and welder joints, attachments, fittings, tip, anvil, clamps, cables, etc. Only very minor wear of the welder tip and anvil contact surfaces were noted after the study was completed. However, during the study, after 82,000 welds a small piece of aluminum was removed from between the tip grooves, even though the weld strength was unaffected by the presence of the aluminum. There were no failures of any mechanical or electrical parts during the study. In addition, primary voltage and current signals of the ultrasonic welder’s power controller were periodically recorded during the weld study and it was determined that there was no change in the electrical behavior of the welder.
APA, Harvard, Vancouver, ISO, and other styles
7

Tamaddoni, Nima, and Andy Sarles. "Characterizing the Sources of Current Generated by a Membrane-Based Hair Cell Sensor." In ASME 2013 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/smasis2013-3141.

Full text
Abstract:
Recently, researchers have developed a method to construct a membrane-based hair cell sensor that generates a measurable current in response to physical disturbance of the hair. Representing the cell membrane, a phospholipid bilayer is formed at the interface of two lipid-encased hydrophilic volumes and a hair is located in a center of one of the volumes act a shaking element. In this work, we study the current generated by free vibration of the hair in a revised hair cell embodiment that uses a hair that is physically supported by the surrounding substrate. The current generated by the sensor is measured by a patch clamp amplifier, and the net charge displaced across the membrane during motion of the hair is computed. Experiments performed with a complete hair cell sensor and various control cases that lack a bilayer indicate that the current measured at 0mV applied across the membrane is due to vibration of the positive electrode that changes the local electromagnetic field. Experiments conducted with both geland liquid-supported membranes indicate that gel-supported membranes have a higher sensitivity of (0.066 pC/mV) than liquid-supported membranes (0.015 pC/mV) as the applied voltage increases. Lastly, the motion of the tip of the hair is imaged using a high-speed camera. This test shows that the hair oscillates at the same frequency observed in the measured current traces, which indicates that transverse bending of the bilayer is the cause for the time rate of change in capacitance in the membrane that produces a voltage-dependent current.
APA, Harvard, Vancouver, ISO, and other styles
8

Zimmermann, Philipp, Kenny Pagel, André Bucht, and Welf-Guntram Drossel. "Design of a Self-Adjusting Terminal Connector Based on Shape Memory Alloys." In ASME 2014 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/smasis2014-7429.

Full text
Abstract:
In electrical engineering terminal connectors are commonly used for connecting and releasing electrical conductors. Within the clamp or contact area the conductor is clamped safely and steadily by a mechanical mechanism to ensure a connection with a consistent quality and safety during the whole period of application. Terminal connectors are used to transfer power in the low and mid voltage range and for transmitting information. The contact force can decrease and the conductor can become loose. This happens due to an inappropriate installation of the conductor in the terminal connector, vibrations, temperature changes as well as the creepage of the contact material, mostly of the conductor. This may lead to damages or fires. Conventional connectors with a self-adjusting capability are not available for all conductor diameters respectively currents and are comparatively cost-intensive. In this paper a self-adjusting terminal connector based on shape memory alloy is developed to enhance a common screw terminal by the integration of a SMA cylinder and the terminal connector is then evaluated regarding its performance and capability.
APA, Harvard, Vancouver, ISO, and other styles
9

Wilson, Jim R., and Neil A. Duncan. "Modelling the Ion Channel Behaviour of Articular Chondrocytes." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-32661.

Full text
Abstract:
All cells have a membrane potential; this voltage difference arises from the different intracellular and extracellular ion concentrations. In excitable tissue the cell membranes contain ion channels which control the movement of ions and hence control the cell’s membrane potential. Extensive measurements of the electrophysiology of excitable cells has allowed considerable understanding of the ion channels. The Hodgkin-Huxley model [1] was developed from measurements on a squid nerve axon, and it quantifies the changes in membrane conductance due to the opening and closing of specific ion channels. This model has been very successful in describing the electrical behaviour of neurons. Ion channels also exist in non-excitable tissue cells. Patch clamp experiments have demonstrated that ion channels in chondrocytes influence cell’s membrane potential [2]; controls the influx of Ca2+ [3] and may regulate cell proliferation [2]. The objective of this research was to develop a model of ion channel behaviour for connective tissue cells based on the Hodgkin-Huxley model, and to apply this model to reported patch clamp measurements of articular chondrocytes.
APA, Harvard, Vancouver, ISO, and other styles
10

O¨zbek, Markus, and Dirk So¨ffker. "About the System Design of a Fuel-Cell / SuperCap Hybrid Powertrain." In ASME 2009 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/detc2009-87354.

Full text
Abstract:
This contribution is based on previous work of the authors and emphasizes the dynamical behavior and design of a fuel-cell driven hybrid powertrain for small vehicles. From a given load profile, a brief discussion will be presented on how to size the fuel-cells and SuperCaps in stationary consideration. The dynamics of the load profile, however put requirements on the system components and affect strongly their life time, in particular the fuel-cells. Especially, the load profile used here which is based on a measured profile from an industrial fork-lift, is very dynamic with large and fast power peaks. Using a Hardware-in-the-Loop test rig of the whole system, two different topologies are used to investigate which one is best suited for a defined industrial application. Further on, conventional led-batteries and SuperCaps with equivalent weight and volume are compared as the energy storage of the system. Finally, a power management to avoid fast transients from the fuel-cells in order to mitigate a fast deterioration is implemented. It will be shown that Super–Caps with the so called Range Extender topology are best suited and an adaptive controller for the output voltage of the DC/DC-converter is used to prevent fast dynamics in order to increase their lifetime.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Whole Cell Voltage Clamp' for particular source types:
Journal articles Dissertations / Theses
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography