Journal articles: 'Winged Bean (Psophocarpus tetragonolobus)'

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OMACHI, Mutsuko, Seiichi HOMMA, and Masao FUJIMAKI. "Triacylglycerol composition of winged bean (Psophocarpus tetragonolobus)." Journal of Nutritional Science and Vitaminology 33, no. 1 (1987): 49–54. http://dx.doi.org/10.3177/jnsv.33.49.

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Gordon, M. H., and K. Mtebe. "Properties of winged bean lipoxygenase (Psophocarpus tetragonolobus)." Food Chemistry 24, no. 3 (January 1987): 219–26. http://dx.doi.org/10.1016/0308-8146(87)90153-1.

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Valíček, Pavel. "The Winged Bean Psophocarpus tetragonolobus: Past, Present, and Future." Outlook on Agriculture 18, no. 4 (December 1989): 160–64. http://dx.doi.org/10.1177/003072708901800404.

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Beans have long been an important source of human and animal nutrition, especially as a source of protein. Recently much attention has been diverted to the possible large-scale cultivation of the winged bean, especially in developing countries. It has many alternative properties, not least of which is that almost the entire plant can be utilized. This article reviews the present status of the crop and the research and development needed for it to achieve its full potential.

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Bassal, Hussein, Othmane Merah, Aqeel M. Ali, Akram Hijazi, and Fawaz El Omar. "Psophocarpus tetragonolobus: An Underused Species with Multiple Potential Uses." Plants 9, no. 12 (December 8, 2020): 1730. http://dx.doi.org/10.3390/plants9121730.

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Natural products, particularly those extracted from plants, have been used as therapy for different diseases for thousands of years. The first written records on the plants used in natural medicine, referred to as “medicinal plants”, go back to about 2600 BC. A thorough and complete understanding of medicinal plants encompasses a multiplex of overlapping and integrated sciences such as botany, pharmacognosy, chemistry, enzymology and genetics. Psophocarpus tetragonolobus, a member of Fabaceae family also called winged bean, is a perennial herbaceous plant characterized by its tuberous roots and its winged pod twinning and a perennial legume rich in proteins, oils, vitamins and carbohydrates. Besides nutrients, winged bean also contains bioactive compounds that have therapeutic activities like anti-oxidant, anti-inflammatory, antinociceptive, antibacterial, antifungal, antiproliferative and cytotoxic activity, a few of which already been reported. This plant can also be used as a medicinal plant for future benefits. With this concept in mind, the present review is designed to shed the light on the interests in the various phytochemicals and pharmacological pharmacognostical aspects of Psophocarpus tetragonolobus.

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Minh, N. P. "Technical variables influencing the seed germination of winged bean (Psophocarpus tetragonolobus) seed." Food Research 5, no. 5 (October 3, 2021): 205–11. http://dx.doi.org/10.26656/fr.2017.5(5).406.

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The demand for sprouted seeds as dietetics and exotic healthy foods has recently increased. Winged bean seeds contain both nutritional composition and antinutrients. In order to convert this seed into healthy food, the winged bean was germinated into a vegetable sprout. In this research, the influence of soaking and sprouting conditions on the physicochemical and sensory attributes of sprouts was observed. Results showed that winged bean should be soaked in water at 34oC in 7 hrs, water to solid 3/1. The soaked seed was drained for 15 mins and incubated at temperature 36oC for 18 hrs. Following these parameters, the winged bean sprout achieved extended length (46.25±0.02 mm), vitamin C (124.13±0.01 mg/100 g), total phenolic content (997.34±1.14 mg GAE/100 g) and overall acceptance (8.94±0.02). Soaking terms for sprouting could improve the nutritional attribute of the winged bean as a functional foodstuff

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Widiani, Nurhaida, Putri Irma, and Marlina Kamelia. "Antioksidan Ekstrak Etanol Buah Kecipir Dengan Metode 1,1-Diphenyl-2-Picrylhidrazyl (DPPH)." Organisms: Journal of Biosciences 2, no. 2 (November 23, 2022): 49–55. http://dx.doi.org/10.24042/organisms.v2i2.12872.

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The fruit of Psophocarpus tetragonolobus L thought to have an antioxidant activity such as seeds and leaves. Ethanol extract from winged bean contains saponins, flavonoids, polyphenols, steroids, and terpenoids. This study aims to determine the antioxidant activity of the ethanol extract of winged bean fruit using the DPPH (1,1-diphenyl-2-picrylhydrazyl). The antioxidant activity test of winged bean extract was carried out at concentrations of 10 ppm, 50 ppm, 100 ppm, and 150 ppm. Added with DPPH (50 ppm) of winged bean extract. Vitamin C was used as a positive control. Absorbance measurement to determine the antioxidant activity of winged bean using a UV-Vis spectrophotometer at a maximum wavelength of 517 nm. The results of this study obtained a qualitative color change in both the winged bean extract and vitamin C. The IC50 value winged bean extract was 98.3229 ppm and included strong antioxidant activity based on the Blois classification.AbstrakBuah kecipir (Psophocarpus tetragonolobus L) diduga memiliki aktivitas antioksidan seperti halnya biji dan daunnya. Ekstrak etanol dari buah kecipir mengandung saponin, flavonoid, polifenolat, steroid dan terpenoid. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak etanol buah kecipir dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Ekstrak buah kecipir didapat dengan cara maserasi menggunakan pelarut etanol. Uji aktivitas antioksidan ekstrak buah kecipir dilakukan pada konsentrasi 10 ppm, 50 ppm, 100 ppm, dan 150 ppm. Ekstrak buah kecipir ditambah dengan DPPH (50 ppm). Vitamin C digunakan sebagai kontrol positif. Pengukuran absorbansi untuk mengetahui aktivitas antioksidan buah kecipir menggunakan spektrofotometer UV-Vis pada panjang gelombang maksimum 517 nm. Hasil penelitian ini didapatkan perubahan warna secara kualitatif baik pada ekstrak buah kecipir dan vitamin C. Nilai IC50 ekstrak buah kecipir senilai 98,3229 ppm dan termasuk memiliki aktivitas antioksidan kuat.

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Maure, Gerson Hans, Muhammad Achmad Chozin, and Edi Santosa. "The Effect of Population Density and Intercropping with Tomato on the Growth and Yield of Winged Bean (Psophocarpus tetragonolobus)." Journal of Tropical Crop Science 6, no. 02 (July 17, 2019): 81–88. http://dx.doi.org/10.29244/jtcs.6.02.81-88.

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Winged bean (Fabaceae) is a tropical crop that has high nutrient content, and almost all parts of the plant are edible. The research aimed to evaluate the growth and production of winged bean in an intercropping system with tomato. The study was conducted at the Cikabayan Experimental Field of Bogor Agricultural University, Bogor, Indonesia, in a randomized block design with three replications during the rainy season of December 2017 to June 2018. Winged beans were planted in high and low population densities and intercropped with three tomato genotypes, “Tora”, F70030081-12-16-3 and “Apel Belgia”. Monoculture winged bean was assigned as control. Tomatoes were planted between the rows of the winged beans in the intercropping treatment. The results showed that the growth of winged bean in monoculture and intercropping systems was not significantly different. The photosynthetic activity of winged bean at three to five weeks after planting remained high, indicating that the vegetative growth was optimal in both systems. Intercropping increased the winged bean number of pods per plant by 12.66-19.52% compared to monoculture, irrespective of population density. Therefore, winged bean could be considered as suitable to grow in intercropping systems.

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Dian Rakhmad, Muhamad Syukur, and Willy Bayuardi Suwarno. "Heritabilitas dan Hubungan Antar Karakter Kuantitatif Kecipir (Psophocarpus tetragonolobus (L.) DC) pada Tiga Lingkungan." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 49, no. 1 (April 30, 2021): 68–74. http://dx.doi.org/10.24831/jai.v49i1.33080.

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Winged bean (Psophocarpus tetragonolobus (L.) DC) is known as tropical soybean with high nutritional content. Lack of information on the variance components and heritability of winged bean breeding is one of the reasons for the lack of winged bean breeding programs in Indonesia. This reseach aimed to obtain information on variance components, heritability and relationship between winged bean traits. The experiment was conducted in three environments, from January 2019 to June 2020. Two environments located in Bogor and one in Palembang. Eleven genotypes of winged bean were tested and 11 traits were observed. The result showed that flowering time and seed number per pod performed wide genetic variability. High heritability was observed on seed number per pod (89.48%), flowering time (83.32%), young pods length (70.97%), and weight of 100 seeds (68.63%). The results of correlation analysis and path analysis showed that young pod productivity (1.07), seed weight per plot (0.81), flowering time (0.29), young pod length (0.17), young pod width (0.12), seed weight per plant (0.09), and 100 seeds weight (0.01) had a positive direct effect on wing bean productivity. Flowering time, seed number per pod, seed weight per plant are suitable characters for selection criteria for breeding high productivity winged bean. Keywords: correlation, genetic variability, path analysis

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Weil, R. R., and A. Samaranayake. "Effects of Winged Bean on a Following Maize Crop." Experimental Agriculture 27, no. 3 (July 1991): 329–38. http://dx.doi.org/10.1017/s0014479700019050.

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SummaryThe effects of winged bean (Psophocarpus tetragonolobus) on a following crop of maize were assessed in two agroecological zones of Sri Lanka. Maize was grown after maize with variable rates of nitrogen fertilizer (0–150 kg N ha−1) or after winged bean or soyabean (Glytine max). Where it could be extrapolated from the nitrogen response curve, the apparent nitrogen contribution by winged bean was 70 kg N ha−1. However, in some cases maize after winged bean yielded more than the maximum yield predicted by the Mitscherlich N-response curve, suggesting the effect of some yield-enhancing mechanism in addition to nitrogen contribution. Where no fertilizer nitrogen was used, maize yields on land which had previously grown winged bean were 52–91% higher than those where maize was the previous crop. Winged bean proved to be an effective green manure crop even when all above-ground plant parts were harvested for food and fodder.

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Sonavane, A. S. "Effect of EMS and SA on seed germination percentage in M1 generation of Psophocarpus tetragonolobus (L.) Dc." International Journal of Bioassays 5, no. 11 (October 31, 2016): 5056. http://dx.doi.org/10.21746/ijbio.2016.11.0012.

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In the present investigation, the two chemical mutagens namely Ethyl methanesulfonate (EMS) and Sodium azide (SA) were used to induce mutations in winged bean (Psophocarpus tetragonolobus (L.) DC.). Winged bean is a potential legume crop of the tropics with high protein and oil content in the seeds. For larger scale cultivation however, the major drawbacks possessed by winged bean should be minimized. The drawbacks such as presence of undesirable antinutritional factors, absence of market demands and labour intensive nature of crop.Hence its production and productivity need to be improved from the present level. A mutation breeding programme was undertaken with the objective of developing improved varieties of winged bean so that it can be popularized among the farmers for its cultivation over large areas. Thus in the present studies, attempts have been made to induce mutations in winged bean and to find out the effect of EMS and SA on seed germination percentage in M1 generation.

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Prasanth, K., I. Sreelathakumary, and VA Celine. "Genetic Divergence Studies in Winged Bean (Psophocarpus tetragonolobus (L.) DC.)." Indian Journal of Plant Genetic Resources 30, no. 1 (2017): 84. http://dx.doi.org/10.5958/0976-1926.2017.00013.4.

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Kotaru, M. "Investigations of antinutritional factors of the winged bean (Psophocarpus tetragonolobus)." Food Chemistry 24, no. 4 (1987): 279–86. http://dx.doi.org/10.1016/0308-8146(87)90103-8.

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Sriwichai, Sasiprapa, Tidarat Monkham, Jirawat Sanitchon, Sanun Jogloy, and Sompong Chankaew. "Dual-Purpose of the Winged Bean (Psophocarpus tetragonolobus (L.) DC.), the Neglected Tropical Legume, Based on Pod and Tuber Yields." Plants 10, no. 8 (August 23, 2021): 1746. http://dx.doi.org/10.3390/plants10081746.

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Winged beans (Psophocarpus tetragonolobus (L.) DC.) are grown as a vegetable legume crop in Thailand. All parts of the plant, including pods, seeds, leaves, flowers, and tubers are edible and are rich in protein and nutrients. Although the major consumption of winged bean is based on pod and tuber yields, only the people of Myanmar and Indonesia utilize winged bean tubers as food materials. The usefulness of the winged bean as an alternative crop for staple food and feed can shed some light on the impact of winged bean. Therefore, the evaluation of the dual purpose of the winged bean based on pod tuber yields is the objective of this study. In this study, ten-winged bean accessions—six accessions obtained from introduced sources and four accessions obtained from local Thai varieties—were laid out in randomized complete block design (RCBD) with three replications at the Agronomy Field Crop Station, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand from September 2019 to April 2020 and from October 2020 to April 2021. Data, including total pod weight, number of pods, pod length, 10-pod weight, and tuber weight were recorded, and the proximate nutrient and mineral contents in the tubers were also determined. The results revealed that the principal effects of year (Y) and genotype (G) were significant for total pod weight and the number of pods. Moreover, the Y × G interactions were principal effects upon the total pod weights and tuber weights. The results indicated that superior genotype and appropriate environmental conditions are key elements in successful winged bean production for both pod and tuber yields. The winged bean accessions W099 and W018 were consistent in both experimental years for pod and tuber yields at 23.6 and 18.36 T/ha and 15.20 and 15.5 T/ha, respectively. Each accession also proved high in tuber protein content at 20.92% and 21.04%, respectively, as well as significant in fiber, energy, and minerals. The results suggest that the winged bean accessions W099 and W018 can be used for dual-purpose winged bean production in Thailand.

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Susanti, Destia, Maya Melati, and Ani Kurniawati. "Identification of Secondary Metabolite Compounds in Two Varieties of Young Winged Beans (Psophocarpus tetragonolobus L.) at Two Harvest Ages." Journal of Tropical Crop Science 9, no. 01 (February 9, 2022): 52–67. http://dx.doi.org/10.29244/jtcs.9.01.52-67.

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The winged bean (Psophocarpus tetragonolobus L.) is a member of the Fabaceae family (beans). Winged bean is developed as a vegetable, and young winged bean pods can be consumed fresh, steamed, fried, or pickled. In Southeast Asia, winged bean pods are generally cooked or consumed as side dishes or salad. Winged bean contains high protein content equivalent to soybean seed. Besides being rich in protein, vitamins, and minerals, the winged bean also has secondary metabolites, including phenolics and flavonoids. This study aimed to identify secondary metabolites in young pods of two varieties, “Fairuz” and “Sandi”, at two different harvest ages of 8 and 10 days after anthesis (DAA) and provide the biological activity on each identified compound. The experiment was conducted at the IPB experimental field at Leuwikopo, Bogor, from September 2020 to February 2021. The identification of secondary metabolites of the young green pods “Fairuz”, and the young purple pods “Sandi”, was performed using the GCMS method at the Regional Health Laboratory (KESDA) DKI Jakarta. The results of the GCMS analysis showed that 1,2-enzenedicarboxylic acid, mono(2-Ethylhexyl) ester was the most abundant compound identified from pods harvested at 8 DAA in both varieties, namely 42.26% in “Fairuz” and 26.66% in “Sandi”. Other compounds, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z, Z, Z) (Linoleic acid ester), were found in “Fairuz”, whereas (9E,12E)-9,12-Octadecadienoic acid (Linoleic acid) was found in “Sandi”; these compounds are hydroxyl group and phenolic glucoside compounds and are found in pods harvested at 10 DAA.

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Shonde, Temitope Esther Olatunde, Moses Adeolu Adebayo, Ayomide Ajoke Bhadmus, Idris Ishola Adejumobi, Olaniyi A. Oyatomi, Benjamin Faloye, and Michael T. Abberton. "Diversity Assessment of Winged Bean [Psophocarpus tetragonolobus (L.) DC.] Accessions from IITA Genebank." Agronomy 13, no. 8 (August 16, 2023): 2150. http://dx.doi.org/10.3390/agronomy13082150.

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The capability of winged bean to support food and nutrition security in sub-Saharan Africa is recurrently being affected by several constraints, which include a lack of genetic improvement. The dearth of adequate information on the level of available genetic diversity in winged bean germplasm has been a major setback in planning appropriate improvement programs. Fifteen winged bean accessions were assessed for genetic diversity using 10 quantitative traits and 10 simple sequence repeat (SSR) markers. The accessions were laid out in RCBD with three replicates for two growing seasons. Leaf samples were obtained from 10 plants representing each accession for SSR marker genotyping. The accessions exhibited significant (p < 0.05) differences for measured traits. Broad-sense heritability estimates varied from 10.31% for days to first plant maturity to 72.67% for pod weight. Pod weight had a positive and significant correlations with pod length (0.53, p < 0.05), pod width (0.70, p < 0.01), and number of seeds per pod (0.64, p < 0.01). However, the number of seeds per pod was negatively correlated with days to maturity (−0.71, p < 0.01). Number of seeds per pod was positively predicted by pod weight, seed thickness, and days to maturity. Cluster analysis delineated the accessions into two distinct groups. Average number of alleles of 4.2, gene diversity of 0.25, and polymorphic information content of 0.22 were recorded. Analysis of molecular variance revealed intra-accession variation of 95% as compared to inter-accession variation of 5%. Two primary genetic groups were identified and only three accessions, namely TPt-6, TPt-126, and TPt-48, showed genetic purity. The results of this study provide the basis for exploiting the existing diversity for winged bean improvement.

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Chankaew, Sompong, Sasiprapa Sriwichai, Teppratan Rakvong, Tidarat Monkham, Jirawat Sanitchon, Sithichoke Tangphatsornruang, Wasitthee Kongkachana, et al. "The First Genetic Linkage Map of Winged Bean [Psophocarpus tetragonolobus (L.) DC.] and QTL Mapping for Flower-, Pod-, and Seed-Related Traits." Plants 11, no. 4 (February 12, 2022): 500. http://dx.doi.org/10.3390/plants11040500.

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Winged bean [Psophocarpus tetragonolobus (L.) DC.] (2n = 2× = 18) is a tropical legume crop with multipurpose usages. Recently, the winged bean has regained attention from scientists as a food protein source. Currently, there is no breeding program for winged bean cultivars. All winged bean cultivars are landraces or selections from landraces. Molecular markers and genetic linkage maps are pre-requisites for molecular plant breeding. The aim of this study was to develop a high-density linkage map and identify quantitative trait loci (QTLs) for pod and seed-related traits of the winged bean. An F2 population of 86 plants was developed from a cross between winged bean accessions W054 and TPT9 showing contrasting pod length, and pod, flower and seed colors. A genetic linkage map of 1384 single nucleotide polymorphism (SNP) markers generated from restriction site-associated DNA sequencing was constructed. The map resolved nine haploid chromosomes of the winged bean and spanned the cumulative length of 4552.8 cM with the number of SNPs per linkage ranging from 36 to 218 with an average of 153.78. QTL analysis in the F2 population revealed 31 QTLs controlling pod length, pod color, pod anthocyanin content, flower color, and seed color. The number of QTLs per trait varied between 1 (seed length) to 7 (banner color). Interestingly, the major QTLs for pod color, anthocyanin content, and calyx color, and for seed color and flower wing color were located at the same position. The high-density linkage map QTLs reported in this study will be useful for molecular breeding of winged beans.

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Maesaroh, Arini, Adi Amurwanto, and Alice Yuniaty. "ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA." Scripta Biologica 1, no. 1 (March 25, 2014): 2. http://dx.doi.org/10.20884/1.sb.2014.1.1.11.

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Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.

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Bhadmus, Ayomide, Michael Abberton, Emmanuel Idehen, Ukoabasi Ekanem, Rajneesh Paliwal, and Olaniyi Oyatomi. "Genetic Diversity Assessment of Winged Bean [Psophocarpus tetragonolobus (L.) DC.] Accessions Using Agronomic and Seed Morphometric Traits." Crops 3, no. 2 (June 7, 2023): 170–83. http://dx.doi.org/10.3390/crops3020017.

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Winged bean [Psophocarpus tetragonolobus (L.) DC.] is an underutilized legume with the potential to contribute to nutrition and food security globally, particularly in sub-Saharan Africa (SSA). However, more attention needs to be paid to exploiting its full potential due to a lack of adequate knowledge of the existing genetic diversity in the available winged bean germplasm. To bridge this gap, thirty accessions of winged beans sourced from the Gene Bank of the International Institute of Tropical Agriculture (IITA), Ibadan, were evaluated for seed morphometric and selected agro-morphological traits at three agro-ecological zones in Nigeria. The data obtained were subjected to analysis of variance (ANOVA), principal component (PC) analysis, cluster analysis, and correlation analysis. Significant differences (p < 0.05) were observed among the accessions for all measured traits. The first two PCs accounted for 88.2% of the variation observed among the accessions for all measured traits. Accessions were grouped into three clusters based on the agro-morphological traits and three clusters under the multi-spectral imaging (MSI) system. TPt-31 had the highest seed yield per plant, pod weight per plant, and early maturity, while TPt-7 had an extended flowering and maturity period, the highest number of pods per peduncle and pods per plant, as well as the lowest seed yield. These accessions could be a good resource for future winged bean improvement programs. The result also confirmed that the MSI system is an invaluable tool for discriminating among accessions of the same crop species. The findings of this study provide insight into the genetic diversity of winged bean germplasm, which could contribute to improving its yield and quality in SSA and globally.

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OHNUKI, Masatoshi, Toyozo SATO, and Tetsuo MAOKA. "Occurrence of leaf spot on winged bean (Psophocarpus tetragonolobus (L.)DC.)." Kyushu Plant Protection Research 35 (1989): 34–36. http://dx.doi.org/10.4241/kyubyochu.35.34.

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V, Kamal Kumar, and ajalakshmi R. "Anatomy and stomatal micromorphology of Psophocarpus tetragonolobus (L.) DC. (Winged Bean)." International Journal of Scientific Research in Biological Sciences 6, no. 1 (February 28, 2019): 233–39. http://dx.doi.org/10.26438/ijsrbs/v6i1.233239.

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YAGI, Fumio, Masahiko SAKAMOTO, Tomoko SAYAWAKI, Kenjiro TADERA, Akira KOBAYASHI, and Kiyotake ISHIHATA. "Lectins in immature pods of winged bean, Psophocarpus tetragonolobus (L.) DC." Agricultural and Biological Chemistry 49, no. 12 (1985): 3575–81. http://dx.doi.org/10.1271/bbb1961.49.3575.

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Yagi, Fumio, Masahiko Sakamoto, Tomoko Sayawaki, Kenjiro Tadera, Akira Kobayashi, and Kiyotake Ishihata. "Lectins in Immature Pods of Winged Bean, Psophocarpus tetragonolobus (L.) DC." Agricultural and Biological Chemistry 49, no. 12 (December 1, 1985): 3575–81. http://dx.doi.org/10.1080/00021369.1985.10867299.

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MURUGISWAMY, B., and M. MADAIAH. "TRYPSIN INHIBITORS OF WINGED BEAN TUBERS (PSOPHOCARPUS TETRAGONOLOBUS): PURIFICATION AND PROPERTIES." Journal of Food Biochemistry 13, no. 4 (August 1989): 253–69. http://dx.doi.org/10.1111/j.1745-4514.1989.tb00398.x.

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Sambudi, Handajani, and Ken A. Buckle. "Characteristics of winged bean (Psophocarpus tetragonolobus) seeds during soaking and boiling." Journal of the Science of Food and Agriculture 57, no. 4 (1991): 585–95. http://dx.doi.org/10.1002/jsfa.2740570411.

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Zusfahair, Zusfahair, Dian Riana Ningsih, Amin Fatoni, Bilalodin Bilalodin, and Aprilia Nafi Nuraini. "The Isolation, Immobilization, and Characterization of Urease from The Seeds of Winged Bean (Psophocarpus tetragonolobus (L.) DC." Molekul 18, no. 1 (March 20, 2023): 70. http://dx.doi.org/10.20884/1.jm.2023.18.1.5932.

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Urease has been utilized in the field of health and industry. Urease is commonly used in the form of free enzyme, so that the utilization is limited. Urease efficiency can be improved using immobilization enzyme. This research aimed to do the urease isolation, immobilization, and characterization from the winged bean seeds. This research was started by determining the amino-acid content of winged bean seeds using the Liquid chromatography-mass spectrometry (LCMS). The winged bean seeds were germinated and extracted. The obtained crude extract’s activity was determined using Nessler reagent and measured using UV-Vis spectrophotometer with the wavelength of 500 nm. The urease of winged bean seeds was immobilized using the alginate matrix. The optimization of urease-immobilized beads could be made through the variations of natrium alginate concentration and beads formation periods in solution CaCl2. Characterization free and immobilized urease were made using the variations of urea substrate concentration, pH, temperature, and also the repeated utilization of immobilized urease. Winged bean seeds are rich with essential amino acid, such as leucine, isoleucine, histidine, phenylalanine, and valine. The urease obtained from the winged bean seeds had the optimum activity in the germination period of 8 days. The urease immobilization showed the optimum condition in the natrium alginate concentration of 5% (w/v) and beads formation period in solution CaCl2 for 60 minutes. The characterization results of free urease and immobilization had the optimum condition at the urea substrate of 0.2 M, and pH 7. Free urease had the optimum temperature of 35 oC, while the immobilized urease had the optimum temperature of 40 oC. The immobilized urease had the utilization stability up to 5 times with the relative activity of 48%. The EDX analysis results showed that the alginate did not contain N, while alginate urease beads contained N as much as 12%.

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Nurpaidah, W. Hermana, and M. Ridla. "Effect of protease in diets containing winged bean seeds (Psophocarpus tetragonolobus) on growth performance of broiler chickens." IOP Conference Series: Earth and Environmental Science 888, no. 1 (November 1, 2021): 012066. http://dx.doi.org/10.1088/1755-1315/888/1/012066.

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Abstract This study aims to determine the effect of adding protease enzyme in diets that contains different levels of winged bean seeds on the growth performance of broiler chickens. In this study, a total of 240 one-day-old chicks were randomly grouped within six treatments and four replicates in a 3×2 factorial arrangements. The first factor was the level of winged bean seeds at 0%, 2.5%, and 5%, while the second was the enzyme treatment with and without protease. The results showed that the additive protease interaction and level of winged bean seeds on the diet had a significant effect on the feed conversion ratio value (P<0.05). Meanwhile, on the feed inateke, final, and body weight gain had no significant effect (p>0.05). Furthermore, the protease treatment significantly decreased feed intake in 35-day old broilers. (P<0.05). Based on these results, the addition of protease enzyme at the levels has the potential to offset the effect of winged bean seeds on growth performance of broiler chickens.

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Pao-la-or, Padej, Boonruang Marungsri, Kakanang Posridee, Ratchadaporn Oonsivilai, and Anant Oonsivilai. "Supercritical CO2 Extraction of Seed Oil from Psophocarpus tetragonolobus (L.) DC.: Optimization of Operating Conditions through Response Surface Methodology and Probabilistic Neural Network." Processes 11, no. 7 (June 27, 2023): 1949. http://dx.doi.org/10.3390/pr11071949.

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For the treatment of menopausal symptoms, nutraceuticals and herbal remedies are thought to be more natural and safer than hormones. Attention has been paid to the winged bean (Psophocarpus tetragonolobus (L.)) DC. seed oil. They are constituted of phytosterols, which may be effective in preventing menopausal symptoms. The purpose was to determine the optimal conditions for supercritical fluid extraction of oleic-rich oil from winged bean seeds. To optimize the condition, the response surface methodology (RSM) and probabilistic neural network (PNN) were utilized. In this research, PNN was used to improve RSM estimation by reducing the number of calculations. The optimized extraction conditions for winged bean seed oil entailed a CO2 flow rate of 21.3 L/h, a pressure of 30 MPa, a temperature of 55 °C, and an extraction time of 90 min. Under these conditions, the extraction process yielded a maximum oil yield of 36.27%. Ultimately, winged bean seed oil included a greater proportion of unsaturated fatty acids such as oleic acid, linoleic acid, and linoleic acid than oil produced using cold pressing or co-solvent extraction.

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GARCIA, V. V., and J. K. PALMER. "Carbohydrates of winged beans, Psophocarpus tetragonolobus (L.) DC*." International Journal of Food Science & Technology 15, no. 5 (June 28, 2007): 477–84. http://dx.doi.org/10.1111/j.1365-2621.1980.tb00966.x.

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O'Garro, Leonard W., and Litta P. Paulraj. "Onion Leaf Blight Caused by Xanthomonas campestris: Alternative Hosts and Resistant Onion Genotypes." Plant Disease 81, no. 9 (September 1997): 978–82. http://dx.doi.org/10.1094/pdis.1997.81.9.978.

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Tomato (Lycopersicon esculentum), pepper (Capsicum annuum), corn (Zea mays), French bean (Phaseolus vulgaris), soybean (Glycine max), winged bean (Psophocarpus tetragonolobus), lima bean (Phaseolus lunatus), field pea (Pisum sativum), moth bean (Phaseolus aconitifolins), and heliconia (Heliconia bihai) were evaluated as alternative hosts of Xanthomonas campestris, the causal agent of a leaf blight of onion (Allium cepa). On legumes, bacterial growth occurred in planta, and visible disease symptoms developed. From 19 commercial onion genotypes screened for leaf blight resistance, two cultivars, H-942 and H-508, were generally free of symptoms and had restricted bacterial growth in planta. These are the first known reports on alternative hosts of the bacterium and on resistance in onion to leaf blight.

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Manoj, N., V. R. Srinivas, B. Satish, Netai C. Singha, and K. Suguna. "Crystallization and preliminary crystallographic analysis of winged bean acidic lectin." Acta Crystallographica Section D Biological Crystallography 55, no. 2 (February 1, 1999): 564–65. http://dx.doi.org/10.1107/s0907444998013018.

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The acidic lectin (WBAII) from the winged bean (Psophocarpus tetragonolobus) binds to the H-antigenic determinant on human erythrocytes and to the T-antigenic disaccharide Gal-β1,3-GalNAc. Two crystal forms of WBAII were obtained in the presence of methyl-α-D-galactose. Form I belongs to space group R3 with unit-cell dimensions a = b = 182.11, c = 44.99 Å and has one dimer in the asymmetric unit. Form II belongs to space group C2 with unit-cell dimensions a = 135.36, b = 127.25, c = 139.98 Å, β = 95.9° and has four dimers in the asymmetric unit. Intensity data were collected to 3.0 Å and to 3.5 Å from crystals of form I and II, respectively. The structures were solved by the molecular-replacement method using the coordinates of the basic form of winged bean lectin.

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Urip, Urip, Nanda Yuniza Pratiwi, Erlin Yustin Tatontos, and Maruni Wiwin Diarti. "Potensi Tepung Biji Kecipir (Psophocarpus tetragonolobus) sebagai Bahan Alternatif Sumber Nitrogen dalam Media Mannitol Salt Agar (MSA) untuk Pertumbuhan Staphylococcus aureus." Bioscientist : Jurnal Ilmiah Biologi 10, no. 2 (December 17, 2022): 1174. http://dx.doi.org/10.33394/bioscientist.v10i2.6138.

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The purpose of this study was to determine the potential of winged bean (Psophocarpus tetragonolobus) seed powder as an alternative medium for Mannitol Salt Agar (MSA) against the growth of Staphylococcus aureus bacteria. This research method is true experiment with Completely Randomized Design (CRD). The experimental unit of this research was winged bean seed flour and Staphylococcus aureus. Winged bean seed flour used with 5 treatments, namely 2 gr, 4 gr, 6 gr, 8 gr, and 10 gr with each replication 5 times. The results showed that the standard MSA as a control showed an average of 3211 colonies, the average colony at a concentration of 2% was 372 colonies, the average colony on a 4% concentration plate was 419 colonies, the average colony on a 6% concentration plate was 544 colonies, the average colony on an 8% concentration plate were 622 colonies, and the average colony on a 10% concentration plate was 816 colonies. The conclusion of this study is winged bean seed powder has the potential as an alternative source of nitrogen in Mannitol Salt Agar (MSA) media for the growth of Staphylococcus aureus.

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USHA, Rajamma, and Manoranjan SINGH. "Proteases of germinating winged-bean (Psophocarpus tetragonolobus) seeds: purification and characterization of an acidic protease." Biochemical Journal 313, no. 2 (January 15, 1996): 423–29. http://dx.doi.org/10.1042/bj3130423.

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Two major classes of protease are shown to occur in germinating winged-bean (Psophocarpus tetragonolobus) seeds, by assaying extracts at pH 8.0 and pH 5.1 with [14C]gelatin as substrate. At pH 8.0, the activity profile of the enzyme shows a steady rise throughout the period of germination, whereas the activity at the acidic pH is very low up to day 5 and then increases sharply reaching a peak on day 11, followed by an equally sharp decline. The winged-bean acidic protease (WbAP) has been purified to apparent homogeneity, as attested by a single protein band on both PAGE and SDS/PAGE. WbAP is a monomeric enzyme with a molecular mass of 35 kDa and a pH optimum of 6.0. It is a thiol protease that does not belong to the papain family and it has tightly bound Ca2+ as shown by 45Ca2+-exchange studies. Besides gelatin and casein, it hydrolyses a 29 kDa winged-bean protein, indicating a prospective physiological role for it in storage-protein mobilization. Immunoblot analysis shows that it occurs only in the seeds and sprouting tubers of this plant and also that it is synthesized in developing seeds just before desiccation. It appears that the newly synthesized enzyme is inactive, and activation takes place around day 6 of germination. However, neither the mechanism of activation nor the signal that triggers it is clearly understood.

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Kant, Achal, R. Nandan, and P. C. Kole. "Agronomic Performance for Tuber Characters in Winged Bean [Psophocarpus tetragonolobus (L.) DC.]." International Journal of Economic Plants 5, no. 2 (May 28, 2018): 086–89. http://dx.doi.org/10.23910/ijep/2018.5.2.0242.

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Scully, B. T., and B. Lakitan. "AN EMPIRICAL SELECTION MODEL FOR THE WINGED BEAN (PSOPHOCARPUS TETRAGONOLOBUS (L)DC.)." Acta Horticulturae, no. 318 (November 1992): 201–8. http://dx.doi.org/10.17660/actahortic.1992.318.25.

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KAILASAPATHY, K., and J. H. MACNEIL. "Baking Studies with Winged Bean (Psophocarpus tetragonolobus L.DC) Flour-Wheat Flour Blends." Journal of Food Science 50, no. 6 (November 1985): 1672–75. http://dx.doi.org/10.1111/j.1365-2621.1985.tb10562.x.

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Patanjali, S. R., S. U. Sajjan, and A. Surolia. "Erythrocyte-binding studies on an acidic lectin from winged bean (Psophocarpus tetragonolobus)." Biochemical Journal 252, no. 3 (June 15, 1988): 625–31. http://dx.doi.org/10.1042/bj2520625.

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An acidic lectin (WBA II) was isolated to homogeneity from the crude seed extract of the winged bean (Psophocarpus tetragonolobus) by affinity chromatography on lactosylaminoethyl-Bio-Gel. Binding of WBA II to human erythrocytes of type-A, -B and -O blood groups showed the presence of 10(5) receptors/cell, with high association constants (10(6)-10(8) M-1). Competitive binding studies with blood-group-specific lectins reveal that WBA II binds to H- and T-antigenic determinants on human erythrocytes. Affinity-chromatographic studies using A-, B-, H- and T-antigenic determinants coupled to an insoluble matrix confirm the specificity of WBA II towards H- and T-antigenic determinants. Inhibition of the binding of WBA II by various sugars show that N-acetylgalactosamine and T-antigenic disaccharide (Thomsen-Friedenreich antigen, Gal beta 1-3GalNAc) are the most potent mono- and di-saccharide inhibitors respectively. In addition, inhibition of the binding of WBA II to erythrocytes by dog intestine H-fucolipid prove that the lectin binds to H-antigenic determinant.

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Okada, Ryo, Eri Kiyota, Hiromitsu Moriyama, Toshiyuki Fukuhara, and Rodrigo A. Valverde. "Molecular and biological properties of an endornavirus infecting winged bean (Psophocarpus tetragonolobus)." Virus Genes 53, no. 1 (October 6, 2016): 141–45. http://dx.doi.org/10.1007/s11262-016-1398-7.

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Matsuda, Tsukasa, Elvin A. Kabat, and Avadhesha Surolia. "Carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus)." Molecular Immunology 26, no. 2 (February 1989): 189–95. http://dx.doi.org/10.1016/0161-5890(89)90101-6.

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Maimako, R. F., O. J. Awakan, A. F. Olaniran, O. P. Olasunkanmi, and O. M. Oluba. "Effects of cooking on antinutrients and antioxidant properties of different accessions of winged bean (Psophocarpus tetragonolobus)." Food Research 6, no. 1 (February 9, 2022): 204–9. http://dx.doi.org/10.26656/fr.2017.6(1).527.

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Asides from its robust nutritional profile, Psophocarpus tetragonolobus also possesses other health-promoting attributes. Hence, this study was designed to investigate the antinutrient content and antioxidant property of five different varieties of raw and cooked P. tetragonolobus. The varieties studied included, the indigenous variety (designated local) and four improved varieties (designated: T11, T31, T125, T154). Antinutrients like phenolic, tannin, glycoside and phytate concentrations were determined using standard methods while 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was carried out to ascertain the antioxidant activity. Phytate, cyanogenic glycosides and tannin levels were significantly (p<0.05) reduced due to cooking in T11 variety. In T125 variety, tannin was significantly lower in the cooked sample compared to the raw sample. However, the phenolic content in the T125 variety increased significantly (p<0.05) due to cooking. There was no significant difference in antioxidant activity between the raw and cooked samples across the five varieties. However, the improved varieties showed higher antioxidant activity compared to the local. Based on these results, the cooking process reduced the phytate, cyanogenic glycosides and tannin levels in the T11 variety while only the tannin level in T125 variety was significantly reduced. However, the antioxidant activity of P. tetragonolobus was not significantly impacted by cooking.

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Nuria, Maulita Cut, Elin Yulinah Sukandar, Asep Gana Suganda, and Muhamad Insanu. "AKTIVITAS INHIBISI ASETILKOLINESTERASE EMPAT JENIS SAYURAN SECARA IN VITRO." JIFFK : Jurnal Ilmu Farmasi dan Farmasi Klinik 16, no. 01 (June 1, 2019): 43. http://dx.doi.org/10.31942/jiffk.v16i01.2928.

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ABSTRACTAcetylcholine is a conducting compound of nerve excitatory (neurotransmitter), while acetylcholinesterase is an enzyme that can hydrolyze the acetylcholine into choline and acetic acid. This reaction is needed so that the nerves can return to take a rest after the activation process, but it can cause damage to the cells in the brain. The most common case is dementia characterized by memory loss. The prevalence of this disease is lower in China and some other Asian countries compared to the United States. It supposed because food consumption commonly by Asians contain vegetables. Extracts derived from vegetables potentially inhibit the activity of acetylcholinesterase (AChE) enzyme which related to chemical contents of the plant. This research aims to investigate the inhibitory activity of acetylcholinesterase enzyme from 4 types of vegetables namely soybean sprouts (bean sprout of Glycine max (L.) Merr.), green bean sprouts (bean sprout of Vigna radiata), carrot (Daucus carota L.), and winged beans (Psophocarpus tetragonolobus (L.) DC.) by in vitro with the Ellman method. Total phenolic and flavonoids contents from fourth types of extracts were also determined. The winged beans extract had the smallest total phenolic and flavonoid contents, but the inhibitory activity against AChE was the biggest. The results showed that IC50 extract of winged beans was 893.86 μg/ml, IC50 carrot extract higher than 1500 μg/ml while the other extracts had no activity. The winged beans extract potential to be further developed as an inhibitor AChE.Keywords : inhibitor of acetylcholinesterase, IC50, vegetables

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Blind, Ariel Dotto, Danilo Fernandes Silva Filho, Josenilce Pereira Pinto, Herodilson Guimaraes da Costa, Diones Lima de Souza, and Jose Nilton Rodrigues Figueiredo. "Germinação de feijão de asa Psophocarpus tetragonolobus em diferentes substratos e espectros luminosos." Revista Agraria Academica 4, no. 6 (November 1, 2021): 153–60. http://dx.doi.org/10.32406/v4n6/2021/153-160/agrariacad.

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Winged bean has a dormancy that is not yet fully clarified during the germination phase. In this sense, the present study sought to evidence germination responses of the species under controlled conditions using 4 types of light (white 80 nm, blue 160 nm, yellow 320 nm and red 460 nm) x 2 substrates (washed sand and plantimax®) with 4 repetitions and 25 seeds. The IVG of wing beans showed better behavior in sand, being 12.8% higher than in the substrate. The root length after 16 days showed better size under the influence of red light, both in sand and in plantimax in the order of 5.72 cm and 6.82 cm, respectively.

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Adegboyega, Taofeek T., Michael T. Abberton, AbdelAziz H. AbdelGadir, Mahamadi Dianda, Busie Maziya-Dixon, Olaniyi A. Oyatomi, Sam Ofodile, and Olubukola O. Babalola. "Nutrient and Antinutrient Composition of Winged Bean (Psophocarpus tetragonolobus (L.) DC.) Seeds and Tubers." Journal of Food Quality 2019 (October 3, 2019): 1–8. http://dx.doi.org/10.1155/2019/3075208.

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Many people in sub-Saharan Africa suffer from protein malnutrition; this results in negative health and economic impacts. Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a tropical underutilized legume with beneficial nutritional characteristics such as high protein content, which may help to alleviate these problems. The proximate composition (fat, moisture content, crude protein, ash, and carbohydrate) and antinutrient (tannin and phytate) level of winged bean seeds and tubers were determined using 50 accessions. In the processed seeds, accession Tpt17 had the highest protein content (40.30%) and Tpt48 the lowest (34.18%). In the unprocessed seeds, Tpt17 also recorded the highest crude protein (31.13%) with Tpt125 having the lowest (28.43%). In the tubers, protein content ranged from 19.07% (Tpt42) to 12.26% (Tpt10). The moisture content in the processed seeds ranged from 8.51% (Tpt42) to 6.72% (Tpt6); in the unprocessed seeds, it was between 8.53% (Tpt53) and 3.76% (Tpt14). In the processed seeds, the values of ash ranged from 4.93% (Tpt126) to 4.45% (Tpt15-4); in the unprocessed seeds, it ranged from 4.98% (Tpt17) to 4.55% (Tpt125). In the processed seeds, the fat content ranged from 18.91% (Tpt51) to 14.09% (Tp43) while in the unprocessed seeds, the values ranged from 19.01% (Tpt15) to 13.87% (Tpt3-B). The crude fiber in the processed samples ranged from 13.82% (Tpt6) to 10.40% (Tpt125) while in the unprocessed seeds, it ranged from 7.29% in Tpt51 to 4.83% in Tpt11. Carbohydrate content in the processed seeds ranged from 26.30% (Tpt3-B) to 20.94% (Tpt125) and 39.76% in Tpt3-B to 34.53% in Tpt18 in the unprocessed seeds. The tannin and phytate contents showed remarkably significant differences. In the tubers harvested, significant variation was observed in the parameters evaluated. Winged bean flour could be formulated into various meals for children and adults to reduce malnutrition in sub-Saharan Africa.

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Slamet, Agus, and Bayu Kanetro. "Potensi Hipolipidemik Yogurt dari Isolat Protein Biji Kecipir (Psophocarpus tetragonolobus) pada Tikus Hiperkolesterol dengan Perlakuan Jumlah Pakan." Agritech 37, no. 1 (March 10, 2017): 1. http://dx.doi.org/10.22146/agritech.16994.

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Protein content of winged bean is almost the same as soybean, but the beany flavor is more poweful than soybean. Therefore the protein of winged bean was isolated prior to use as raw material of yogurt. This research was aimed to determine the potency of hypocholestrolemic activity of yogurt protein isolate of winged bean through in vivo bioassay by using Sprague Dawley male rats. The treatments of the research were yogurt feed treatment with concentration of yogurt 0 (standard feed without yogurt as a control), 2, and 4 g yogurt/day as low and high concentration treatment respectively for 4th weeks after hypercholesterol feed treatment for 1 week. The blood lipid profile of rats, including triglyceride, cholesterol total, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) cholesterol were analysed on the 2nd and 4th weeks for the yogurt feed treatment while for before yogurt feed treatment, the evaluation were based on the adaptation phase and the 1st week for hypercholesterol phase. The result of this research showed that the blood triglyceride, cholesterol total, LDL increased, and the blood HDL decreased in hypercholesterol phase before yogurt feed treatment. The potency of hypocholestrolemic of yogurt from protein isolate of winged bean was shown by the decreasing of blood triglyceride, cholesterol total, LDL and increasing the HDL cholesterol after the yogurt feed treatment with low and high concentration. That indicated that yogurt that was made of protein isolate of winged bean could reduced cholesterol. ABSTRAKBiji kecipir memiliki kadar protein yang hampir sama dengan kedelai, namun bau langunya lebih tajam daripada kedelai, sehingga perlu diisolasi proteinnya sebelum digunakan sebagai bahan baku yogurt. Tujuan penelitian ini adalah menentukan potensi hipokolesterolemik yogurt isolat proteun biji kecipir melalui uji biologis in vivo menggunakan tikus jantan Sprague Dawley. Perlakuan penelitian ini adalah perlakuan pakan yogurt dengan konsentrasi 0 (pakan standar tanpa penambahan yogurt sebagai kontrol), 2, dan 4 g yogurt/hari berturut-turut sebagai perlakuan konsentrasi rendah dan tinggi selama 4 minggu perlakuan pakan yogurt sesudah pemberian pakan hiperkolesterol selama 1 minggu. Profil lipida darah tikus meliputi kadar trigliserida, total kolesterol, kolesterol High Density Lipoprotein (HDL), dan Low Density Lipoprotein (LDL) dianalisis pada minggu ke 2 dan 4 minggu selama perlakuan pakan yogurt dan sebelum perlakuan pakan yogurt yaitu pada fase pemeliharaan adaptasi dan 1 minggu pada fase pemeliharan hiperkolesterol. Hasil penelitian ini menunjukkan bahwa trigliserida, total kolesterol, dan kolesterol LDL meningkat dan kolesterol HDL menurun selama fase pemberian pakan hiperkolesterol sebelum perlakuan pakan yogurt. Potensi hipokolesterol yogurt isolat protein biji kecipir ditunjukkan dengan penurunan trigliserida, total kolesterol, dan kolesterol LDL, serta peningkatan kolesterol HDL sesudah perlakuan pakan yogurt dengan konsentrasi rendah maupun tinggi. Hal tersebut mengindikasikan bahwa yogurt isolat protein biji kecipir mampu menurunkan kolesterol.

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Mirdhe, Romana M., and H. C. Lakshman H. C. Lakshman. "Efficacy of AM fungi and its Interaction with Rhizobium in Winged bean (Psophocarpus tetragonolobus (L) D.C)." International Journal of Scientific Research 2, no. 4 (June 1, 2012): 22–24. http://dx.doi.org/10.15373/22778179/apr2013/10.

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Purwati, Catur Suci, and Danang Riyadi. "Perubahan Suhu, pH, Protein Kasar, dan Serat Kasar pada Fermentasi Biji Kecipir (Psophocarpus tetragonolobus) dan Tepung Jagung dengan Level Jamur Trichoderma viride yang Berbeda." Jurnal Ilmu dan Teknologi Peternakan Tropis 5, no. 2 (December 16, 2018): 45. http://dx.doi.org/10.33772/jitro.v5i3.4766.

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ABSTRAKTujuan penelitian ini adalah mengetahui proses fermentasi yang tepat digunakan untuk meningkatkan kualitas protein kasar dan serat kasar pada biji kecipir dan menentukan level terbaik penambahan jamur Trichoderma viride yang mampu meningkatkan kualitas protein kasar dan serat kasar pada biji kecipir sehingga mampu meningkatkan kualitas dan tingkat kecernaan. Produk yang akan dihasilkan pada penelitian ini adalah alaternatif pakan fermentasi biji kecipir (Psophocarpus tetragonolobus) untuk peternak unggas. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu P0 = biji kecipir dengan 10% tepung jagung tanpa fermentasi; P1 = Biji kecipir dengan 10% tepung jagung + 0,1% jamur Trichoderma viride (Tv); P2 = biji kecipir dengan 10% tepung jagung + 0,2% Tv; dan P3 = biji kecipir dengan 10% tepung jagung + 0,3% Tv. Materi yang digunakan adalah biji kecipir yang dihaluskan digunakan sebagai substrat dan dihomogenkan dengan cara diaduk. Masing-masing sampel perlakukan ditimbang sebanyak 100 g, kemudian dimasukkan ke dalam kantong plastik dengan ketebalan 2 cm dan dilubangi agar tercipta suasana aerob. Selanjutnya diinkubasikan dalam ruang fermentor pada suhu 30oC selama 7 hari. Masing-masing perlakuan diulang sebanyak 3 kali. Peubah yang diamati pH, suhu, protein kasar dan serat kasar. Berdasarkan penelitian ini dapat disimpulkan bahwa terjadi perubahan suhu, penurunan pH, serta kenaikan pada protein kasar dan penurunan serat kasar.Kata kunci: biji kecipir, Trichoderma viride, pH, suhu, protein, serat kasar ABSTRACTThe purpose of this study was to determine the proper fermentation process used to improve the quality of crude protein and crude fiber in winged beans and the best level of addition of Trichoderma viride fungus which could improve the quality of crude protein and crude fiber in winged beans to improve feed quality and digestibility. The result product of this study is an alternative fermented winged bean (Psophocarpus tetragonolobus) feed for poultry farmers. This study utilized completely randomized design with four treatments. P0 = Unfermented winged beans with cornstarch, P1 = winged beans with 10% cornstarch + 0,1% Trichoderma viride fungus (Tv), P2 = winged beans with 10% cornstarch + 0,2% Tv, P3 = winged beans with 10% cornstarch + 0,3% Tv. The material used were powdered winged beans as substrate then homogenized by stirring. Each sample was weighed 100 g, then put into a plastic bag with a 2 cm thickness and perforated to create an aerobic atmosphere. Subsequently, the sample was incubated in a fermenter room at 30oC for 7 days. Each treatment was repeated 3 times. Variables observed for pH, temperature, crude protein, and crude fiber. The result of this study concluded that there were change in temperature, decrease in pH, increase in crude protein, and decrease in crude fiber.Keywords: winged beans, Trichoderma viride, pH, temperature, protein, crude fiber

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Hlosrichok, Anirut, and Ratchaneewan Aunpad. "Antioxidant potential of gamma ray irradiated winged bean (Psophocarpus tetragonolobus) seed protein hydrolysate." Functional Foods in Health and Disease 13, no. 4 (April 18, 2023): 208. http://dx.doi.org/10.31989/ffhd.v13i4.1090.

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Background: Winged bean or Psophocarpus tetragonolobus (WB) seeds have high protein content and could be applied as a source of antioxidant proteins and peptides. The utilization of gamma rays in plant protein extraction provides consumers with a safe and harmless technology.Objective: To determine the efficiency of gamma rays in the protein extraction process of WB seeds and investigate the antioxidant activity, cytotoxicity, cytoprotective effect of the gamma ray irradiated WB (WB-G) seed protein hydrolysate.Methods: WB seeds were irradiated with gamma ray (WB-G) and total protein were extracted by acid-base extraction method. The total protein was further hydrolyzed with alcalase enzyme to obtain the protein hydrolysate. The antioxidant activity was evaluated by DPPH and ABTS assay. The cytotoxicity and cytoprotective effect were determined by MTT assay. The cellular reactive oxygen species was further analyzed by ACFDA assay.Results: The protein extracted from WB-G seeds showed higher protein yield and antioxidant activity than that of non-irradiated seeds. After hydrolysis with alcalase enzyme at 55°C for 6 hours, WB-G protein hydrolysate with degree of hydrolysis of 90.00±0.91% exhibited strong antioxidant activity with IC50 value of 10.30±0.02 µg/ml and 3.10±0.06 µg/ml as measured by DPPH and ABTS assay, respectively. Moreover, it showed no toxicity toward L929 mouse fibroblast cells and IPEC-J2 cell lines and can reduce the damage caused by H2O2. As determined in cellular reactive oxygen species by H2-DCFDA assay, the WB-G protein hydrolysate can reduce the level of ROS and subsequently the damage of cell by H2O2. Conclusions: This study demonstrated that gamma ray irradiation on the WB seeds can increase the protein yield and its protein hydrolysate exhibited promising antioxidant activity and cytoprotective effect on cells against H2O2 damage. It might be developed as an alternative food or feed supplement.Keywords:Psophocarpus tetragonolobus; Gamma rays; Protein hydrolysate; Antioxidant activity; Cytoprotective effect

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Esan, Adewale Michael, Charles Ojo Olaiya, Samson Tolulope Adedire, Folusho Eunice Bamigboye, and Kehinde Olugboyega Soetan. "Biochemical and Nutritional Importance of Winged Bean (Psophocarpus tetragonolobus (L.)) on Wistar Rats." International Journal of Food Science and Agriculture 4, no. 2 (May 27, 2020): 174–82. http://dx.doi.org/10.26855/ijfsa.2020.06.009.

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Khalili, R. Mohd Adzim, S. Emynur Shafekh, A. H. Norhayati, I. Mohd Fatahudin, R. Rahimah, H. Norkamalia, and A. Nor Azimah. "Total Phenolic Content and In vitro Antioxidant Activity of Winged Bean (Psophocarpus tetragonolobus)." Pakistan Journal of Nutrition 12, no. 5 (April 15, 2013): 416–22. http://dx.doi.org/10.3923/pjn.2013.416.422.

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Wong, Q. N., F. Massawe, and S. Mayes. "Improving winged bean (Psophocarpus tetragonolobus) productivity: an analysis of the determinants of productivity." Acta Horticulturae, no. 1102 (September 2015): 83–88. http://dx.doi.org/10.17660/actahortic.2015.1102.9.

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B., A. Lawal, A. Azeez M., O. Egedegbe G., M. Omogoye A., A. Raji I., and K. Akintola E. "Biomass accumulation and yield expression among winged bean (Psophocarpus tetragonolobus L. DC) accessions." Journal of Cereals and Oilseeds 10, no. 2 (August 31, 2019): 16–22. http://dx.doi.org/10.5897/jco2019.0197.

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Journal articles on the topic 'Winged Bean (Psophocarpus tetragonolobus)'

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