Relevant bibliographies by topics / Wound healing assay

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Wound healing assay'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 January 2023

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Journal articles on the topic "Wound healing assay"

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Wu, Shang-Ying, Yung-Shin Sun, Kuan-Chen Cheng, and Kai-Yin Lo. "A Wound-Healing Assay Based on Ultraviolet Light Ablation." SLAS TECHNOLOGY: Translating Life Sciences Innovation 22, no. 1 (July 10, 2016): 36–43. http://dx.doi.org/10.1177/2211068216646741.

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Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration takes place in the following wound-healing process. To study this cellular behavior, various kinds of wound-healing assays are developed. In these assays, a “wound,” or a “cell-free region,” is created in a cell monolayer mechanically, chemically, optically, or electrically. These assays are useful tools in studying the effects of certain physical or chemical stimuli on the wound-healing process. Most of these methods have disadvantages such as creating wounds of different sizes or shapes, yielding batch-to-batch variation, and damaging the coating of the cell culture surface. In this study, we used ultraviolet (UV) lights to selectively kill cells and create a wound out of a cell monolayer. A comparison between the current assay and the traditional scratch assay was made, indicating that these two methods resulted in similar wound-healing rates. The advantages of this UV-created wound-healing assay include fast and easy procedure, high throughput, and no direct contact to cells.
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Maini, P. K., D. L. S. McElwain, and D. Leavesley. "Travelling waves in a wound healing assay." Applied Mathematics Letters 17, no. 5 (May 2004): 575–80. http://dx.doi.org/10.1016/s0893-9659(04)90128-0.

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Luan, Shaoliang, Rui Hao, Yuanchen Wei, Deyong Chen, Beiyuan Fan, Fengliang Dong, Wei Guo, Junbo Wang, and Jian Chen. "A microfabricated 96-well wound-healing assay." Cytometry Part A 91, no. 12 (November 20, 2017): 1192–99. http://dx.doi.org/10.1002/cyto.a.23286.

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van der Meer, Andries D., Kim Vermeul, André A. Poot, Jan Feijen, and István Vermes. "A microfluidic wound-healing assay for quantifying endothelial cell migration." American Journal of Physiology-Heart and Circulatory Physiology 298, no. 2 (February 2010): H719—H725. http://dx.doi.org/10.1152/ajpheart.00933.2009.

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Endothelial migration is an important process in the formation of blood vessels and the repair of damaged tissue. To study this process in the laboratory, versatile and reliable migration assays are essential. The purpose of this study was to investigate whether the microfluidic version of the conventional wound-healing assay is a useful research tool for vascular science. Endothelial cells were seeded in a 500-μm-wide microfluidic channel. After overnight incubation, cells had formed a viable and confluent monolayer. Then, a wound was generated in this monolayer by flushing the channel with three parallel fluid streams, of which the middle one contained the protease trypsin. By analyzing the closing of the wound over time, endothelial cell migration could be measured. Although the migration rate was two times lower in the microfluidic assay than in the conventional assay, an identical 1.5-times increase in migration rate was found in both assays when vascular endothelial growth factor (VEGF165) was added. In the microfluidic wound-healing assay, a stable gradient of VEGF165 could be generated at the wound edge. This led to a two-times increase in migration rate compared with the untreated control. Finally, when a shear stress of 1.3 Pa was applied to the wound, the migration rate increased 1.8 times. In conclusion, the microfluidic assay is a solid alternative for the conventional wound-healing assay when endothelial cell migration is measured. Moreover, it offers unique advantages, such as gradient generation and application of shear stress.
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Shabestani Monfared, Ghazal, Peter Ertl, and Mario Rothbauer. "Microfluidic and Lab-on-a-Chip Systems for Cutaneous Wound Healing Studies." Pharmaceutics 13, no. 6 (May 26, 2021): 793. http://dx.doi.org/10.3390/pharmaceutics13060793.

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Cutaneous wound healing is a complex, multi-stage process involving direct and indirect cell communication events with the aim of efficiently restoring the barrier function of the skin. One key aspect in cutaneous wound healing is associated with cell movement and migration into the physically, chemically, and biologically injured area, resulting in wound closure. Understanding the conditions under which cell migration is impaired and elucidating the cellular and molecular mechanisms that improve healing dynamics are therefore crucial in devising novel therapeutic strategies to elevate patient suffering, reduce scaring, and eliminate chronic wounds. Following the global trend towards the automation, miniaturization, and integration of cell-based assays into microphysiological systems, conventional wound healing assays such as the scratch assay and cell exclusion assay have recently been translated and improved using microfluidics and lab-on-a-chip technologies. These miniaturized cell analysis systems allow for precise spatial and temporal control over a range of dynamic microenvironmental factors including shear stress, biochemical and oxygen gradients to create more reliable in vitro models that resemble the in vivo microenvironment of a wound more closely on a molecular, cellular, and tissue level. The current review provides (a) an overview on the main molecular and cellular processes that take place during wound healing, (b) a brief introduction into conventional in vitro wound healing assays, and (c) a perspective on future cutaneous and vascular wound healing research using microfluidic technology.
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Saha, Susmita, Deepjyoti Bhattacharjee, Anwesha Saha, Gahin De, Partha Saha, and S. K. Sil. "Wound healing promoting activity of Earthworm, Eutyphoeus gammiei (Beddard): in vitro studies on human skin keratinocyte cell line (HaCat)." Journal of Drug Delivery and Therapeutics 8, no. 6 (November 15, 2018): 155–58. http://dx.doi.org/10.22270/jddt.v8i6.2036.

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Earthworm, Eutyphoeus gammiei, homogenate (EGH) was screened for wound healing activity on human keratinocyte cell line, HaCat, by cell proliferation and migration assays. The maximum proliferation and migration of keratinocyte cells were observed at the dose of 25μg/ml. As cell proliferation and migration are key factors for wound healing, the study clearly suggests the potential role of earthworm species Eutyphoeus gammiei on wound healing. Keywords: Eutyphoeus gammiei, Keratinocyte, MTT assay, scratch assay.
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Lin, Jin-Young, Kai-Yin Lo, and Yung-Shin Sun. "Effects of Substrate-Coating Materials on the Wound-Healing Process." Materials 12, no. 17 (August 29, 2019): 2775. http://dx.doi.org/10.3390/ma12172775.

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The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it difficult to compare results from different assays. In this paper, a modified barrier wound-healing assay was reported for studying the wound-healing process on different substrates in one single petri dish. In short, half of a dish was covered with the tape, and coating materials, poly-l-lysine and gelatin, were applied to the surface. After peeling off the tape, half of the surface was coated with the desired material. Then a customized barrier was placed inside the dish to create the wound. The results indicated that surface coating did not affect cell proliferation/viability, and the wound-healing rate increased in coated surfaces compared to uncoated ones. The present study provides a platform for further understanding the mechanisms of substrate coating-dependent wound-healing processes.
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Lara-Reyes, Jesús Antonio, Mariana Giezi Jimenez-Buendia, Gonzalo Emiliano Aranda-Abreu, Deissy Herrera-Covarrubias, Clara Luz Sampieri, Arnoldo Aquino-Galvez, Jorge Manzo-Denes, María Elena Hernández-Aguilar, and Fausto Rojas-Durán. "Razor scrape assay, an alternative variation to wound and healing assays." MethodsX 7 (2020): 101135. http://dx.doi.org/10.1016/j.mex.2020.101135.

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Andrgie, Abegaz Tizazu, Haile Fentahun Darge, Tefera Worku Mekonnen, Yihenew Simegniew Birhan, Endiries Yibru Hanurry, Hsiao-Ying Chou, Chih-Feng Wang, Hsieh-Chih Tsai, Jen Ming Yang, and Yen-Hsiang Chang. "Ibuprofen-Loaded Heparin Modified Thermosensitive Hydrogel for Inhibiting Excessive Inflammation and Promoting Wound Healing." Polymers 12, no. 11 (November 6, 2020): 2619. http://dx.doi.org/10.3390/polym12112619.

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Hydrogels have been investigated as ideal biomaterials for wound treatment owing to their ability to form a highly moist environment which accelerates cell migration and tissue regeneration for prompt wound healing. They can also be used as a drug carrier for local delivery, and are able to activate immune cells to enhance wound healing. Here, we developed heparin-conjugated poly(N-isopropylacrylamide), an injectable, in situ gel-forming polymer, and evaluated its use in wound healing. Ibuprofen was encapsulated into the hydrogel to help reduce pain and excessive inflammation during healing. In addition to in vitro studies, a BALB/c mice model was used to evaluate its effect on would healing and the secretion of inflammatory mediators. The in vitro assay confirmed that the ibuprofen released from the hydrogel dramatically reduced lipopolysaccharide-induced inflammation by suppressing the production of NO, PGE2 and TNF-α in RAW264.7 macrophages. Moreover, an in vivo wound healing assay was conducted by applying hydrogels to wounds on the backs of mice. The results showed that the ibuprofen-loaded hydrogel improved healing relative to the phosphate buffered saline group. This study indicates that ibuprofen loaded in an injectable hydrogel is a promising candidate for wound healing therapy.
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Zhu, Na, Shuai Meng, Jianchun Li, Tianjun Liu, and Saeed Rohani. "Fenugreek Extract-Loaded Polycaprolacton/Cellulose Acetate Nanofibrous Wound Dressings for Transplantation of Unrestricted Somatic Stem Cells: An In Vitro and In Vivo Evaluation." Journal of Biomedical Nanotechnology 18, no. 9 (September 1, 2022): 2216–26. http://dx.doi.org/10.1166/jbn.2022.3424.

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Complex pathophysiology of diabetic wounds causes a delayed wound healing response. Advanced wound dressing materials that deliver biochemical cues are of particular interest in wound healing research. Here, we developed a dual-function delivery vehicle for drug and cell delivery applications to treat diabetic wounds. The delivery system was developed via electrospinning of polycaprolacton/cellulose acetate solution containing fenugreek extract. The produced delivery vehicle was characterized using microstructural studies, cell viability assay, cytoprotection assay, cell migration assay, In Vitro anti-inflammatory assay, free radical scavenging assay, tensile strength studies, swelling studies, and protein adsorption test. Scaffolds were then seeded with 30000 unrestricted somatic stem cells and transplanted into the rat model of excisional diabetic wound. Wound healing assay showed that the co-delivery of fenugreek extract and unrestricted somatic stem cells led to a substantial improvement in the healing activity of electrospun dressings, as evidenced by higher wound contraction, epithelial thickness, and collagen deposition in this group compared with other experimental groups. Gene expression analysis showed that dual-function delivery system could increase the expression level of VEGF, b-FGF, and collagen type II genes. Furthermore, the tissue expression level of IL-1β and glutathione peroxidase genes was significantly reduced in this group compared with other groups. This study shows that the developed system may be considered as a potential treatment modality for diabetic wounds in the clinic.
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Dissertations / Theses on the topic "Wound healing assay"

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Morgaenko, Katsiarina. "Sledování migrace buněk v mikrofluidním systému metodou „Scratch Wound Healing Assay“." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2019. http://www.nusl.cz/ntk/nusl-403757.

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Tato diplomová práce se zabývá popisem principů kultivace embryonálních fibroblastových buněk myší (3T3), lidských endoteliálních buněk odebraných z pupečníkové žily (HUVEC) a epiteliálních buněk vaječníku čínského křečka (CHO) v mikrofluidních systémech simulujících kapiláry. Byly provedeny literární rešerše v oblasti realizací experimentu “Scratch Wound Healing Assay” v mikrofluidních systémech s použitím fibroblastů a endotheliálních buněk. V práci jsou dále popsány principy konfokální a fluorescenční mikroskopie a metody zpracování obrazů pro sledování buněčné migrace. Experimentální nastavení pro mikrofluidní realizaci “Scratch Wound Healing Assay” s použitím trypsinu – EDTA pro vytvoření rýhy, a konfokálního mikroskopu Leica TCS SP8 X pro následující snímání pořízených dat bylo navrženo a otestováno s dostatečným počtem opakování. Vhodný algoritmus pro analýzu buněčné migrace byl napsán v programovacím prostředí Matlab. Závěrem této práce je diskuze získaných výsledků.
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Yang, Yongliang. "Emergent Leader Cells in Collective Cell Migration in In Vitro Wound Healing Assay." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/332896.

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Collective cell migration is critical for various physiological and pathological processes. In vitro wound healing assay has been widely used to study collective cell migration due to its technical simplicity and ability of revealing the complexity of collective cell migration. This project studies the function and importance of leader cells, the cells pulling cell monolayer migrating into free space, in endothelium and skin epithelial regeneration via plasma lithography enhanced in vitro wound healing assay. Despite leader cells have been identified in in vitro wound healing assays, little is known about their regulation and function on collective cell migration. First, I investigated the role of leader cells in endothelial cell collective migration. I found that the leader cell density is positively related with the cell monolayer migration rates. Second, we used this knowledge to study the effects of arsenic treatment on skin regeneration via in vitro wound healing assay. We found that low concentration of arsenic treatment can accelerate the keratinocyte monolayer migration. We further found that arsenic affected cell migration by modulating leader cell density through Nrf2 signaling pathway. As a conclusion of these studies, we evaluated the function of leader cells in collective cell migration, and elucidated the mechanism of arsenic treatment on skin regeneration.
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Post, Hannah [Verfasser], and Jennifer E. [Akademischer Betreuer] Hundt. "Development and testing of a novel ex vivo assay for studying “pathological” wound healing in human skin / Hannah Post ; Akademischer Betreuer: Jennifer E. Hundt." Lübeck : Zentrale Hochschulbibliothek Lübeck, 2021. http://d-nb.info/1227903251/34.

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Mun, Kyu-Shik. "Monitoring Cell Behaviors on Variety of Micropatterns Created with Biodegradable Polymer." University of Cincinnati / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1457426363.

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Johnston, Stuart T. "Mathematical models for quantifying collective cell behaviour." Thesis, Queensland University of Technology, 2017. https://eprints.qut.edu.au/109793/1/Stuart_Johnston_Thesis.pdf.

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Collective behaviour is critical to a variety of biological and ecological processes, including tumour invasion, wound healing and spreading of invasive species. This thesis investigated mathematical models of collective cell behaviour, with an aim to develop techniques for applying these models to experimental data to obtain quantitative insight from experiments, and to develop novel models that accurately incorporate cellular mechanisms. We determined various appropriate techniques to extract quantitative information about cell movement and cell proliferation, given particular experimental data. We also developed novel mathematical models that accurately describe the average behaviour of cells undergoing birth, death and movement.
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Treloar, Katrina K. "Mathematical models for collective cell spreading." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/86960/1/Katrina_Treloar_Thesis.pdf.

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Collective cell spreading is frequently observed in development, tissue repair and disease progression. Mathematical modelling used in conjunction with experimental investigation can provide key insights into the mechanisms driving the spread of cell populations. In this study, we investigated how experimental and modelling frameworks can be used to identify several key features underlying collective cell spreading. In particular, we were able to independently quantify the roles of cell motility and cell proliferation in a spreading cell population, and investigate how these roles are influenced by factors such as the initial cell density, type of cell population and the assay geometry.
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Pillai, Mahesh Ramachandran. "Deciphering the Link Between Polychlorinated Biphenyls, Immune Function and Exercise." Bowling Green State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510140839084446.

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Lawson, Brodie Alexander James. "Cell migration and proliferation on homogeneous and non-homogeneous domains : modelling on the scale of individuals and populations." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/61066/1/Brodie_Lawson_Thesis.pdf.

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Cell migration is a behaviour critical to many key biological effects, including wound healing, cancerous cell invasion and morphogenesis, the development of an organism from an embryo. However, given that each of these situations is distinctly different and cells are extremely complicated biological objects, interest lies in more basic experiments which seek to remove conflating factors and present a less complex environment within which cell migration can be experimentally examined. These include in vitro studies like the scratch assay or circle migration assay, and ex vivo studies like the colonisation of the hindgut by neural crest cells. The reduced complexity of these experiments also makes them much more enticing as problems to mathematically model, like done here. The primary goal of the mathematical models used in this thesis is to shed light on which cellular behaviours work to generate the travelling waves of invasion observed in these experiments, and to explore how variations in these behaviours can potentially predict differences in this invasive pattern which are experimentally observed when cell types or chemical environment are changed. Relevant literature has already identified the difficulty of distinguishing between these behaviours when using traditional mathematical biology techniques operating on a macroscopic scale, and so here a sophisticated individual-cell-level model, an extension of the Cellular Potts Model (CPM), is been constructed and used to model a scratch assay experiment. This model includes a novel mechanism for dealing with cell proliferations that allowed for the differing properties of quiescent and proliferative cells to be implemented into their behaviour. This model is considered both for its predictive power and used to make comparisons with the travelling waves which result in more traditional macroscopic simulations. These comparisons demonstrate a surprising amount of agreement between the two modelling frameworks, and suggest further novel modifications to the CPM that would allow it to better model cell migration. Considerations of the model’s behaviour are used to argue that the dominant effect governing cell migration (random motility or signal-driven taxis) likely depends on the sort of invasion demonstrated by cells, as easily seen by microscopic photography. Additionally, a scratch assay simulated on a non-homogeneous domain consisting of a ’fast’ and ’slow’ region is also used to further differentiate between these different potential cell motility behaviours. A heterogeneous domain is a novel situation which has not been considered mathematically in this context, nor has it been constructed experimentally to the best of the candidate’s knowledge. Thus this problem serves as a thought experiment used to test the conclusions arising from the simulations on homogeneous domains, and to suggest what might be observed should this non-homogeneous assay situation be experimentally realised. Non-intuitive cell invasion patterns are predicted for diffusely-invading cells which respond to a cell-consumed signal or nutrient, contrasted with rather expected behaviour in the case of random-motility-driven invasion. The potential experimental observation of these behaviours is demonstrated by the individual-cell-level model used in this thesis, which does agree with the PDE model in predicting these unexpected invasion patterns. In the interest of examining such a case of a non-homogeneous domain experimentally, some brief suggestion is made as to how this could be achieved.
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Riahi, Reza. "Engineered Molecular Probes for Systematic Studies of Cellular Response in Collective Cell Migration." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/312515.

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The investigation of complex biological processes, such as wound healing, cell migration, cancer cell invasion, and gene regulatory networks can be benefited tremendously by novel biosensing techniques with high stability and spatiotemporal resolution. In particular, molecular probes with qualities including high stability, sensitivity, and specificity are highly sought-after for long-term monitoring of gene expression in individual cells. Among different single-cell analysis techniques oligonucleotide optical probes is a promising detection method to monitor the dynamics of cellular responses. Herein, the design and optimization of double-stranded LNA probes are first investigated. With alternating DNA/LNA monomers for optimizing the stability and specificity, we show that the probe is highly stable in living cells and is capable of detecting changes in gene expression induced by external stimuli. Using dsLNA probes we then demonstrate the novel approaches to monitor the spatiotemporal gene expression response during cell injury. Our results also suggest a potential autoregulatory role of Nrf2 in injury induced EMT. We also show that the signaling level of dsLNA probe can serve as a molecular signature for the leader cells near the wound which allows us to track the behaviors of leader cells during collective cell migration. Finally multimodal GNR-LNA approach is proposed to map spatiotemporal gene expression profile and reveal dynamic characteristics of heat shock response in photothermal operations.
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Yeh, Chun Chih, and 葉軍志. "Three-dimensional wound healing assay." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107CGU05114064%22.&searchmode=basic.

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Book chapters on the topic "Wound healing assay"

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Martinotti, Simona, and Elia Ranzato. "Scratch Wound Healing Assay." In Methods in Molecular Biology, 225–29. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/7651_2019_259.

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Freitas, Juliano T., Ivan Jozic, and Barbara Bedogni. "Wound Healing Assay for Melanoma Cell Migration." In Methods in Molecular Biology, 65–71. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1205-7_4.

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Zabary, Yishaia, and Assaf Zaritsky. "A MATLAB Pipeline for Spatiotemporal Quantification of Monolayer Cell Migration." In Bioimage Data Analysis Workflows ‒ Advanced Components and Methods, 175–206. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-76394-7_8.

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AbstractIn this chapter we present a MATLAB-based computational pipeline for the quantification of monolayer migration assays. Wound healing assay (or scratch assay) is a commonly used in vitro assay to assess collective cell migration. Our pipeline outputs traditional and spatiotemporal readouts that quantify the group migration properties and was previously used for a screen that included thousands of time-lapse sequences. You will learn how to execute the pipeline, the principles behind the design and implementation choices we made, pitfalls, tips, and tricks in using it.
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Ganguli-Indra, Gitali. "Protocol for Cutaneous Wound Healing Assay in a Murine Model." In Stem Cells and Tissue Repair, 151–59. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1435-7_12.

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Vašinková, Markéta, Michal Krumnikl, Zuzana Mikulková, Petr Gajdoš, and Eva Kriegová. "Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay." In Advances in Intelligent Networking and Collaborative Systems, 380–92. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-14627-5_39.

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Zemkewicz, John L., Racheal G. Akwii, Constantinos M. Mikelis, and Colleen L. Doçi. "Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay." In Methods in Molecular Biology, 1–11. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0845-6_1.

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Cardona, A., L. Ariza-Jiménez, D. Uribe, J. Arroyave, and F. M. Cortés-Mancera. "Automatic Image Segmentation Method for In Vitro Wound Healing Assay Quantitative Analysis." In VI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014, 381–84. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13117-7_98.

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Wiegand, C., J. Tittelbach, U. C. Hipler, and P. Elsner. "Water-Filtered Infrared A Irradiation: From Observations in Clinical Studies to Complex In Vitro Models." In Water-filtered Infrared A (wIRA) Irradiation, 203–12. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-92880-3_17.

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AbstractSuccessful treatment of recalcitrant common hand and foot warts in a prospective randomized controlled blind trial using wIRA and PDT has been reported. In addition, in wound healing wIRA is mostly investigated in vitro based on the resolution of mechanical damage to confluent cell layers using the “scratch wound assay.” The latter enables the direct measurement of cell migration and regeneration of the cell layer. Preliminary studies for wIRA effects on wound closure in vitro have shown beneficial effects of single 10 min treatments. Although cellular processes induced and mediators involved still need to be elucidated, it is apparent that the observed clinical benefits of wIRA on wound healing can be investigated in vitro using adequate models and experimental settings. The next step is to employ 3D skin models for morphological investigations closely simulating in vivo conditions.
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Tong, Junfeng, and Zhixiang Wang. "Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay." In Methods in Molecular Biology, 159–63. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7219-7_12.

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Molinie, Nicolas, and Alexis Gautreau. "Directional Collective Migration in Wound Healing Assays." In Methods in Molecular Biology, 11–19. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7701-7_2.

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Conference papers on the topic "Wound healing assay"

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Topman, Gil, Orna Sharabani-Yosef, and Amit Gefen. "A Method for Quantitative Analysis of the Kinematics of Fibroblast Migration in a Monolayer Wound Model." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53070.

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A wound healing assay is simple but effective method to study cell migration in vitro. Cell migration in vitro was found to mimic migration in vivo to some extent [1,2]. In wound healing assays, a “wound” is created by either scraping or mechanically crushing cells in a monolayer, thereby forming a denuded area. Cells migrate into the denuded area to complete coverage, and thereby “heal” the wound. Micrographs at regular time intervals are captured during such experiments for analysis of the process of migration.
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Cohen Maslaton, Shir, and Natan T. Shaked. "Wound healing assay of two competing cell types with dry mass measurement." In Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, edited by Pietro Ferraro, Monika Ritsch-Marte, Simonetta Grilli, and Christoph K. Hitzenberger. SPIE, 2019. http://dx.doi.org/10.1117/12.2526841.

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Wei, Y., F. Chen, T. Zhang, D. Chen, X. Jia, J. Tong, J. Wang, W. Guo, and J. Chen. "A tubing-free microfluidic wound-healing assay quantifying vascular smooth muscle cell migration." In TRANSDUCERS 2015 - 2015 18th International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2015. http://dx.doi.org/10.1109/transducers.2015.7181291.

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Bise, R., T. Kanade, Zhaozheng Yin, and Seung-il Huh. "Automatic cell tracking applied to analysis of cell migration in wound healing assay." In 2011 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2011. http://dx.doi.org/10.1109/iembs.2011.6091525.

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Mondal, N., D. Mondal, C. RoyChaudhuri, A. Barui, S. Dhara, and J. Chatterjee. "A simple and sensitive cytosensor based electrical characterization of in vitro wound healing assay for keratinocytes." In 2011 IEEE/NIH 5th Life Science Systems and Applications Workshop (LiSSA). IEEE, 2011. http://dx.doi.org/10.1109/lissa.2011.5754152.

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Galeano Zea, July A., Cesar Bedoya, Cardona Andrés, Fabián Cortés-Mancera, Patrick Sandoz, and Artur Zarzycki. "Modified position-referenced microscopy for the analysis of low-magnification biological events: a case of study in the wound healing assay with a human hepatoma cell line." In Latin America Optics and Photonics Conference. Washington, D.C.: OSA, 2016. http://dx.doi.org/10.1364/laop.2016.ltu4a.52.

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Maistrenko, Lesia, Olga Iungin, Oleksii Savchuk, and Olena Okhmat. "Collagen matrices from leather industry wastes for biomedical application." In The 8th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2020. http://dx.doi.org/10.24264/icams-2020.ii.15.

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Modern biomedical science is challenged to develop new wound healing drugs. The collagen-containing wastes of leather industry could be the rich source of collagen products for further use in biomedical science. The aim of this research was to find the best source of collagen between limed pelt, delimed pelt and fleshings of cattle hides, and to prepare it for the use as a matrix for further microbiological studies. Collagen was extracted with 0.5 M acetic acid and 5 mM EDTA. The purity of the extracted collagen was checked by gel-electophoresis (SDS-PAGE). The rate of growth and crystal violet assay of laboratory strains (S. aureus, P. aeruginosa) were used for microbiological evaluation of obtained collagen matrices. The delimed pelt provided the highest concentration of collagen and the greatest volume of collagen products. All obtained collagen products were applicable as matrices for microbial cells growth. The applicability of collagen products from leather industry wastes for biomedical studies in Ukraine was shown.
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Šeklić, Dragana, Milena Jovanović, Nevena Milivojević, and Marko Živanović. "PLATINUM(IV) COMPLEX AND ITS CORRESPONDING LIGAND SUPPRESS CELL MOTILITY AND PROMOTE EXPRESSION OF FRIZZLED-7 RECEPTOR IN COLORECTAL CANCER CELLS." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac, 2021. http://dx.doi.org/10.46793/iccbi21.288s.

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Suppression of cell movement is an imperative in the effectiveness of future generations of chemotherapeutics. Frizzled 7 receptor (FZD7), as the first protein of Wnt/β-catenin signaling cascade, plays a significant role in regulation of cell differentiation, proliferation, and cell migration. This study aimed to investigate the potential effects of platinum (IV) complex: [PtCl4 (dbu-S, S-eddp)] – C1, and its corresponding ligand – L1 on cell movement, as well as the FZD7 expression and localization after treatments on two human colorectal carcinoma cell lines (HCT-116, SW-480). A Wound healing assay was used to examine cell migration, while FZD7 protein expression was examined by immunofluorescence. Chemical compounds, especially L1, reduced cell motility of both tested cell lines. They showed a particularly good effect on HCT-116 cells, increasing protein expression of the antimigratory marker FZD7 whose localization was observed on the cell membrane of HCT-116 cells. Suppression of cell movement was significantly lower in SW-480 cells after treatments, when compared to HCT-116, with an obvious decrease of FZD7 receptor expression and its localization in the cytoplasm of these cells. Our results indicate that among the examined treatments, the ligand showed more significant results in the suppression of HCT-116 cell movement, most likely through the stimulation of differentiation, which is indicated by the promotion of FZD7 expression.
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Ahmad, Salma, Hanan Nazar, Nouralhuda Alatieh, Maryam Al-Mansoob, Zainab Farooq, Muna Yusuf, and Allal Ouhtit. "Validation of Novel Transcriptional Targets that Underpin CD44-promoted breast cancer cell invasion." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0153.

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Introduction: Breast cancer (BC) is the most common cancer worldwide, and metastasis is its worst aspect and the first cause of death. Metastasis is a multistep process, where an invasion is a recurring event. The process of BC cell invasion involves three major factors, including cell adhesion molecules (CAM), proteinases and Growth factors.CD44, a family of CAM proteins and the hyaluronic acid (HA) cell surface receptor, acts as cell differentiation, cell migration/invasion and apoptosis regulator. Rationale: We have previously established a tetracycline (Tet)-OFF-regulated expression system, both in vitro and in vivo (Hill et al, 2006). As a complementary approach, the highly metastatic MDA-MB-231 BC cells expressing high levels of endogenous CD44s (the standard form of CD44), was cultured in the presence and absence of 50 µg/ml of HA. RNA samples were isolated from both cell experimental models, and microarray analysis (12K CHIP from Affymetrix) was applied. More than 200 CD44s transcriptional target genes were identified and were sub-divided into groups of genes based on their function: cell motility, cytoskeletal organization, ability to degrade ECM, and cell survival. Hypothesis: Among these 200 identified genes, we selected seven genes (ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1, and SOD2) and hypothesized that based on evidence from literature, these genes are potential novel targets of CD44-downstream signaling mediating BC cell invasion. Specific Aims: Pursuant to this goal, we proposed the following objectives: 1- Structural validation of ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1 and SOD2 as novel transcriptional targets of CD44/HA-downstream signaling at both RNA and Protein level using reverse transcription polymerase chain reaction (RT-PCR) and Western Blot respectively. 2-Functional validation of ICAP-1, KYNU, AHR, SIRT1, SRSF8, PRAD1and SOD2 as novel transcriptional targets that underpin CD44-promoted BC cell migration using wound healing assay after the transfection with siRNA. Innovation/Consclusion: This study validated seven transcriptional targets of CD44/HA-downstream signaling promoting BC cell invasion. Ongoing experiments aim to dissect the signaling pathways that link CD44 activation by HA to the transcription of these seven genes.
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"Research of zinc-containing humic compounds wound-healthing properties and zinc assay content in biomaterial after their topical application." In Seventh International Conference on Humic Innovative Technologies "Humic substances and technologies for resilience" (HIT – 2022). NP CBR "Humus Sapiens", 2022. http://dx.doi.org/10.36291/hit.2022.055.

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