Relevant bibliographies by topics / Wound scratch assay

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Wound scratch assay'

Author: Grafiati
Published: 4 June 2025
Last updated: 23 June 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Wound scratch assay.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Wound scratch assay"

1

Alishahedani, Mohammadali E., Manoj Yadav, Katelyn J. McCann, et al. "Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing." PLOS ONE 16, no. 6 (2021): e0253669. http://dx.doi.org/10.1371/journal.pone.0253669.

Full text
Abstract:
The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to ‘heal’ the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted.
APA, Harvard, Vancouver, ISO, and other styles
2

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Wound Healing Potential of Biofield Energy Treatment in HaCaT Cell Line Using Scratch Assay." Global Research Journal of Public Health and Epidemiology 5, no. 5 (2018): 136–42. https://doi.org/10.5281/zenodo.2560883.

Full text
Abstract:
Wounds may be acute, subacute, or chronic and are the common clinical life entities. It is a complex biochemical process to restore the cell structures that depends upon cell proliferation and migration, mainly by keratinocytes and fibroblast cells. The aim of the present study was to evaluate the effect of the Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect&reg;) in the HaCaT cell line (Human keratinocytes) and DMEM (Dulbecco&rsquo;s Modified Eagle Medium) for wound healing action using scratch assay (48 hours) against positive control i.e., recombinant Human Epidermal Growth Factor (Hu-EGF, 30 ng/mL). In vitro scratch assay monitored proliferation and migration of keratinocytes in the scratched wounded area using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEM group have significantly higher percentage of keratinocytes migration i.e., 35.4%, while no migration was monitored in the Biofield Energy Treated HaCaT cells as compared with the baseline control group. Additionally, the percentage of scratch area was significantly decreased by 7.3% in the Biofield Energy Treated DMEM, while an increased scratched area was reported by 14% in the Biofield Energy Treated cells. Hence, the results using scratch assay showed a promising scientific approach to differentiate the Biofield Energy Treatment on cells or media for their wound healing potential. The data concluded that The Trivedi Effect&reg; has the significant capability in wound healing activity in DMEM as compared with the HaCaT cell line with respect to the cell migration and reduced wound scratched area. Consciousness Energy Healing Treatment (The Trivedi Effect&reg;) can be used as a complementary and alternative approach to improve the cellular migration and proliferation and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent. <strong>Source</strong>: https://www.trivedieffect.com/science/wound-healing-potential-of-biofield-energy-treatment-in-hacat-cell-line-using-scratch-assay http://springjournals.net/grjphe/springjournals.netgrjphearticlesindex=4trivedietal &nbsp;
APA, Harvard, Vancouver, ISO, and other styles
3

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Wound Healing Potential of Biofield Energy Treatment in HaCaT Cell Line Using Scratch Assay." Global Research Journal of Public Health and Epidemiology 5, no. 5 (2019): 136–42. https://doi.org/10.5281/zenodo.2566679.

Full text
Abstract:
Wounds may be acute, subacute, or chronic and are the common clinical life entities. It is a complex biochemical process to restore the cell structures that depends upon cell proliferation and migration, mainly by keratinocytes and fibroblast cells. The aim of the present study was to evaluate the effect of the Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect&reg;) in the HaCaT cell line (Human keratinocytes) and DMEM (Dulbecco&rsquo;s Modified Eagle Medium) for wound healing action using scratch assay (48 hours) against positive control i.e., recombinant Human Epidermal Growth Factor (Hu-EGF, 30 ng/mL). In vitro scratch assay monitored proliferation and migration of keratinocytes in the scratched wounded area using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEM group have significantly higher percentage of keratinocytes migration i.e., 35.4%, while no migration was monitored in the Biofield Energy Treated HaCaT cells as compared with the baseline control group. Additionally, the percentage of scratch area was significantly decreased by 7.3% in the Biofield Energy Treated DMEM, while an increased scratched area was reported by 14% in the Biofield Energy Treated cells. Hence, the results using scratch assay showed a promising scientific approach to differentiate the Biofield Energy Treatment on cells or media for their wound healing potential. The data concluded that The Trivedi Effect&reg; has the significant capability in wound healing activity in DMEM as compared with the HaCaT cell line with respect to the cell migration and reduced wound scratched area. Consciousness Energy Healing Treatment (The Trivedi Effect&reg;) can be used as a complementary and alternative approach to improve the cellular migration and proliferation and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent. &nbsp; https://www.trivedieffect.com/science/wound-healing-potential-of-biofield-energy-treatment-in-hacat-cell-line-using-scratch-assay http://springjournals.net/grjphe/springjournals.netgrjphearticlesindex=4trivedietal
APA, Harvard, Vancouver, ISO, and other styles
4

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Wound Healing Activity of Consciousness Energy Healing Treatmenton HFF-1 Cells and DMEM Using Scratch Assay." Investigative Dermatology and Venereology Research 4, no. 1 (2018): 50–54. https://doi.org/10.5281/zenodo.3374350.

Full text
Abstract:
The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect&reg;) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco&rsquo;s Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded area. The scratched area was monitored after 24 hours of wound closure in the Biofield Energy Treated HFF-1 cells and the Biofield Energy Treated DMEM groups, and the representative photomicrographs were taken in each wells using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEMwassignificantly higher percentage of fibroblast migration i.e. 51.8%, while the migration was altered in the Biofield Energy Treated HFF-1 cell line compared to the baseline control group. In addition to, the percentage of scratch area was significantly decreased by 2.7% in the Biofield Energy Treated DMEM group, while it was increased by 12% in the Biofield Treated cells group compared to the baseline control group. Overall, the experimental results concluded that The Trivedi Effect&reg; has the significant capacity and wide implications in wound healing activity via cell culture media, DMEM as compared with the HFF-1 cell line directly. Biofield Energy Healing would be a complementary and alternative medicine that can be used against burn injury cases, acute wound, skin regeneration, eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc. <strong>Source:</strong> https://www.trivedieffect.com/science/wound-healing-activity-of-consciousness-energy-healing-treatmenton-hff-1-cells-and-dmem-using-scratch-assay/ https://www.ommegaonline.org/article-details/Wound-Healing-Activity-of-Consciousness-Energy-Healing-Treatmenton-HFF-1-Cells-and-DMEM-Using-Scratch-Assay-/2036
APA, Harvard, Vancouver, ISO, and other styles
5

Amalia, Latifa, Retno Murwanti, Triana Hertiani, and Kurnia Rahayu Purnomo. "Evaluation of the Potential In Vitro effects of Plantago major L. on Wound Healing in Human Umbilical Vein Endothelial Cells (HUVEC)." Indonesian Journal of Cancer Chemoprevention 15, no. 2 (2025): 87. https://doi.org/10.14499/indonesianjcanchemoprev15iss2pp87-95.

Full text
Abstract:
The treatment of skin wounds remains a major concern in the field of medicine, particularly in the case of chronic wounds resulting from various disorders such as diabetes. The utilization of herbs or herbal preparations for the purpose of healing skin wounds presents a therapeutic challenge within the realm of traditional medicine. Plantago major L. is known to have bioactive compounds that have wound healing activity such as aucubin. This study aimed to determine the in vitro wound healing potential of Plantago major L. extract (PLE). The study involved several assays, including phytochemical examination of PLE using TLC, cell viability testing using MTT assay, and wound healing testing using scratch assay on human umbilical vein endothelial cells (HUVEC). The results confirmed the presence of aucubin as one of the compounds in PLE. It was observed that PLE with 125 μg/mL exhibited the highest wound closure percentage at 90.66%. This study shows that PLE possesses wound healing capabilities.Keywords: Plantago major L., PLE, cytotoxic assay, wound scratch assay, HUVEC.
APA, Harvard, Vancouver, ISO, and other styles
6

Balko, Stefan, Evan Kerr, Edward Buchel, Sarvesh Logsetty, and Afshin Raouf. "A Robust and Standardized Approach to Quantify Wound Closure Using the Scratch Assay." Methods and Protocols 6, no. 5 (2023): 87. http://dx.doi.org/10.3390/mps6050087.

Full text
Abstract:
The scratch assay is an in vitro assay that allows for high-throughput quantification of wound closure by keratinocytes and fibroblasts with relative ease. However, this assay is amenable to experimental variables, which can result in false-positive and false-negative data, making the interpretation of such data difficult. Also, data variability decreases the sensitivity of the scratch assay. Here, we identify important sources of data variation in the scratch assay and provide rational mitigation strategies that enable robust and highly reproducible quantification of scratch width and area, and ultimately the scratch closure rates. By eliminating these sources of variability, the sensitivity of the scratch assay is enhanced, thereby allowing for identification of dependent variables with wide-ranging impacts on wound closure in a robust and standardized manner.
APA, Harvard, Vancouver, ISO, and other styles
7

Jana, Snehasis, and Mahendra Kumar Trivedi. "Wound Healing Activity of Consciousness Energy Healing Treatmenton HFF-1 Cells and DMEM Using Scratch Assay." Investigative Dermatology and Venereology Research 4, no. 1 (2018): 50–54. http://dx.doi.org/10.15436/2381-0858.18.2036.

Full text
Abstract:
The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded area. The scratched area was monitored after 24 hours of wound closure in the Biofield Energy Treated HFF-1 cells and the Biofield Energy Treated DMEM groups, and the representative photomicrographs were taken in each wells using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEMwassignificantly higher percentage of fibroblast migration i.e. 51.8%, while the migration was altered in the Biofield Energy Treated HFF-1 cell line compared to the baseline control group. In addition to, the percentage of scratch area was significantly decreased by 2.7% in the Biofield Energy Treated DMEM group, while it was increased by 12% in the Biofield Treated cells group compared to the baseline control group. Overall, the experimental results concluded that The Trivedi Effect® has the significant capacity and wide implications in wound healing activityvia cell culture media, DMEM as compared with the HFF-1 cell line directly. Biofield Energy Healing would be a complementary and alternative medicine that can be used against burn injury cases, acute wound, skin regeneration, eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.
APA, Harvard, Vancouver, ISO, and other styles
8

Freiesleben, Sara H., Jens Soelberg, Nils T. Nyberg, and Anna K. Jäger. "Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/9480791.

Full text
Abstract:
The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.
APA, Harvard, Vancouver, ISO, and other styles
9

Azis, Huwaida Abdul, Muhammad Taher, Deny Susanti, and Zainul Amiruddin Zakaria. "IN VITRO WOUND HEALING ACTIVITY OF SAPIUM INDICUM WILLD LEAF EXTRACTS." African Journal of Traditional, Complementary and Alternative Medicines 12, no. 2 (2015): 126–32. http://dx.doi.org/10.21010/ajtcam.v12i2.19.

Full text
Abstract:
Background: Sapium indicum Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community.&#x0D; Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model. &#x0D; Methods: AESI and EESI were prepared using maceration techniques. The extracts were subjected to phytochemical screening, cytotoxicity (MTT assay) and scratch assay.&#x0D; Results: AESI contained saponins and tannins while EESI contained triterpenes, tannins and proteins. Based on MTT assay, AESI and EESI exhibited IC50 = 16.3 and 70.9 µg/mL, respectively. In the scratch assay, EESI caused significant (P&lt; 0.05) concentration-dependent migration on 3T3 L1 cells whereas AESI exerted concentration-independent effect. &#x0D; Conclusion: The leaves of S. indicum exhibited wound healing potential and contained phytochemicals that may contribute to the activity. These findings would support potential use of S. indicum as wound healing plant.
APA, Harvard, Vancouver, ISO, and other styles
10

Radstake, Wilhelmina E., Kiran Gautam, Cynthia Van Rompay, et al. "Comparison of in vitro scratch wound assay experimental procedures." Biochemistry and Biophysics Reports 33 (March 2023): 101423. http://dx.doi.org/10.1016/j.bbrep.2023.101423.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Wound scratch assay"

1

Morgaenko, Katsiarina. "Sledování migrace buněk v mikrofluidním systému metodou „Scratch Wound Healing Assay“." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2019. http://www.nusl.cz/ntk/nusl-403757.

Full text
Abstract:
Tato diplomová práce se zabývá popisem principů kultivace embryonálních fibroblastových buněk myší (3T3), lidských endoteliálních buněk odebraných z pupečníkové žily (HUVEC) a epiteliálních buněk vaječníku čínského křečka (CHO) v mikrofluidních systémech simulujících kapiláry. Byly provedeny literární rešerše v oblasti realizací experimentu “Scratch Wound Healing Assay” v mikrofluidních systémech s použitím fibroblastů a endotheliálních buněk. V práci jsou dále popsány principy konfokální a fluorescenční mikroskopie a metody zpracování obrazů pro sledování buněčné migrace. Experimentální nastavení pro mikrofluidní realizaci “Scratch Wound Healing Assay” s použitím trypsinu – EDTA pro vytvoření rýhy, a konfokálního mikroskopu Leica TCS SP8 X pro následující snímání pořízených dat bylo navrženo a otestováno s dostatečným počtem opakování. Vhodný algoritmus pro analýzu buněčné migrace byl napsán v programovacím prostředí Matlab. Závěrem této práce je diskuze získaných výsledků.
APA, Harvard, Vancouver, ISO, and other styles
2

Johnston, Stuart T. "Mathematical models for quantifying collective cell behaviour." Thesis, Queensland University of Technology, 2017. https://eprints.qut.edu.au/109793/1/Stuart_Johnston_Thesis.pdf.

Full text
Abstract:
Collective behaviour is critical to a variety of biological and ecological processes, including tumour invasion, wound healing and spreading of invasive species. This thesis investigated mathematical models of collective cell behaviour, with an aim to develop techniques for applying these models to experimental data to obtain quantitative insight from experiments, and to develop novel models that accurately incorporate cellular mechanisms. We determined various appropriate techniques to extract quantitative information about cell movement and cell proliferation, given particular experimental data. We also developed novel mathematical models that accurately describe the average behaviour of cells undergoing birth, death and movement.
APA, Harvard, Vancouver, ISO, and other styles
3

Lawson, Brodie Alexander James. "Cell migration and proliferation on homogeneous and non-homogeneous domains : modelling on the scale of individuals and populations." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/61066/1/Brodie_Lawson_Thesis.pdf.

Full text
Abstract:
Cell migration is a behaviour critical to many key biological effects, including wound healing, cancerous cell invasion and morphogenesis, the development of an organism from an embryo. However, given that each of these situations is distinctly different and cells are extremely complicated biological objects, interest lies in more basic experiments which seek to remove conflating factors and present a less complex environment within which cell migration can be experimentally examined. These include in vitro studies like the scratch assay or circle migration assay, and ex vivo studies like the colonisation of the hindgut by neural crest cells. The reduced complexity of these experiments also makes them much more enticing as problems to mathematically model, like done here. The primary goal of the mathematical models used in this thesis is to shed light on which cellular behaviours work to generate the travelling waves of invasion observed in these experiments, and to explore how variations in these behaviours can potentially predict differences in this invasive pattern which are experimentally observed when cell types or chemical environment are changed. Relevant literature has already identified the difficulty of distinguishing between these behaviours when using traditional mathematical biology techniques operating on a macroscopic scale, and so here a sophisticated individual-cell-level model, an extension of the Cellular Potts Model (CPM), is been constructed and used to model a scratch assay experiment. This model includes a novel mechanism for dealing with cell proliferations that allowed for the differing properties of quiescent and proliferative cells to be implemented into their behaviour. This model is considered both for its predictive power and used to make comparisons with the travelling waves which result in more traditional macroscopic simulations. These comparisons demonstrate a surprising amount of agreement between the two modelling frameworks, and suggest further novel modifications to the CPM that would allow it to better model cell migration. Considerations of the model’s behaviour are used to argue that the dominant effect governing cell migration (random motility or signal-driven taxis) likely depends on the sort of invasion demonstrated by cells, as easily seen by microscopic photography. Additionally, a scratch assay simulated on a non-homogeneous domain consisting of a ’fast’ and ’slow’ region is also used to further differentiate between these different potential cell motility behaviours. A heterogeneous domain is a novel situation which has not been considered mathematically in this context, nor has it been constructed experimentally to the best of the candidate’s knowledge. Thus this problem serves as a thought experiment used to test the conclusions arising from the simulations on homogeneous domains, and to suggest what might be observed should this non-homogeneous assay situation be experimentally realised. Non-intuitive cell invasion patterns are predicted for diffusely-invading cells which respond to a cell-consumed signal or nutrient, contrasted with rather expected behaviour in the case of random-motility-driven invasion. The potential experimental observation of these behaviours is demonstrated by the individual-cell-level model used in this thesis, which does agree with the PDE model in predicting these unexpected invasion patterns. In the interest of examining such a case of a non-homogeneous domain experimentally, some brief suggestion is made as to how this could be achieved.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Wound scratch assay"

1

Cory, Giles. "Scratch-Wound Assay." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Martinotti, Simona, and Elia Ranzato. "Scratch Wound Healing Assay." In Methods in Molecular Biology. Springer US, 2019. http://dx.doi.org/10.1007/7651_2019_259.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Zabary, Yishaia, and Assaf Zaritsky. "A MATLAB Pipeline for Spatiotemporal Quantification of Monolayer Cell Migration." In Bioimage Data Analysis Workflows ‒ Advanced Components and Methods. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-76394-7_8.

Full text
Abstract:
AbstractIn this chapter we present a MATLAB-based computational pipeline for the quantification of monolayer migration assays. Wound healing assay (or scratch assay) is a commonly used in vitro assay to assess collective cell migration. Our pipeline outputs traditional and spatiotemporal readouts that quantify the group migration properties and was previously used for a screen that included thousands of time-lapse sequences. You will learn how to execute the pipeline, the principles behind the design and implementation choices we made, pitfalls, tips, and tricks in using it.
APA, Harvard, Vancouver, ISO, and other styles
4

Vašinková, Markéta, Michal Krumnikl, Zuzana Mikulková, Petr Gajdoš, and Eva Kriegová. "Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay." In Advances in Intelligent Networking and Collaborative Systems. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-14627-5_39.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Wiegand, C., J. Tittelbach, U. C. Hipler, and P. Elsner. "Water-Filtered Infrared A Irradiation: From Observations in Clinical Studies to Complex In Vitro Models." In Water-filtered Infrared A (wIRA) Irradiation. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-92880-3_17.

Full text
Abstract:
AbstractSuccessful treatment of recalcitrant common hand and foot warts in a prospective randomized controlled blind trial using wIRA and PDT has been reported. In addition, in wound healing wIRA is mostly investigated in vitro based on the resolution of mechanical damage to confluent cell layers using the “scratch wound assay.” The latter enables the direct measurement of cell migration and regeneration of the cell layer. Preliminary studies for wIRA effects on wound closure in vitro have shown beneficial effects of single 10 min treatments. Although cellular processes induced and mediators involved still need to be elucidated, it is apparent that the observed clinical benefits of wIRA on wound healing can be investigated in vitro using adequate models and experimental settings. The next step is to employ 3D skin models for morphological investigations closely simulating in vivo conditions.
APA, Harvard, Vancouver, ISO, and other styles
6

Castellano-Pellicena, Irene, and M. Julie Thornton. "Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0648-3_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

S. Weeks, Benjamin, Ruiqin Fu, and Mohammad Zaidi. "Vitamin C Promotes Wound Healing: The Use of in Vitro Scratch Assays to Assess Re-Epithelialization." In Cell Physiology - Annual Volume 2023 [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.111517.

Full text
Abstract:
Vitamin C contributes to the health of the cardiovascular, immunological and nervous system and also supports healthy bone, lung, and skin function and wound healing. The mechanism of action of vitamin C in human health is as diverse as its targets and effects. For example, vitamin C functions as an antioxidant, signals changes in gene expression, plays a role in protein metabolism, and serves a cofactor in several biosynthetic pathways including collagen synthesis. Here in this chapter we will explore the role of vitamin C in support of improved wound healing during the re-epithelialization stage. While vitamin C supports wound healing in early stages by reducing inflammation, vitamin C continues to support wound healing by promoting collagen synthesis and epithelial cell migration in the re-epithelialization stage. The re-epithelialization stage of wound healing has been modeled and investigated using an in vitro scratch-assay in which a monolayer of epithelial cells is scratched to create a gap or void in the monolayer to represent the wound. The rate of epithelial cell migration back across this gap to re-establish the monolayer can then be used as a model and measurement of the re-epithelialization stage of wound healing. Again, this Chapter will review the literature on both a) the uses of in vitro scratch assays to investigate the mechanism of vitamin C enhanced epithelial cell migration and b) the potential uses of the in vitro scratch assay to study the bioavailability and absorption of liposomal vitamin C.
APA, Harvard, Vancouver, ISO, and other styles
8

"Scratch Wound Assays." In Biomedical Image Analysis Recipes in MATLAB®. John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118657546.ch5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Virador, Gabriel, Lisa Patel, Matthew Allen, et al. "Systematically Assessing Natural Compounds’ Wound Healing Potential with Spheroid and Scratch Assays." In Advances in Experimental Medicine and Biology. Springer International Publishing, 2022. http://dx.doi.org/10.1007/5584_2022_727.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Yeo, Aimin, Yi Bing Aw, and Pamela Mok. "An In Vitro Delayed Scratch Closure Assay with Features of Chronic Wounds." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/7651_2024_578.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Wound scratch assay"

1

Bittner, Nicholas M., Ghoulem Ifrene, Amanda Haage, and Aiyana Montclair. "Proof of Concept for a Device to Standardize Cellular Migration Assay Experiments." In 2025 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2025. https://doi.org/10.1115/dmd2025-1075.

Full text
Abstract:
Abstract One of the most common techniques for investigating the migration of human cells in culture is a “scratch assay” where a pipette tip is dragged through a layer of cells creating a “wound”. Migration is then observed as the cells move back into the area that was cleared. While common, this method has drawbacks. It requires a seasoned hand to perform the “scratch”, it leaves behind wounded remains of cells creating a unique microenvironment, and by design, the scratch is jagged and ununiform in nature. This research demonstrates the functionality of a cheap, easy to implement 3D printed device that mimics this assay. Its benefits include needing virtually no training to use, creating uniform areas for migration, and limiting cell death.
APA, Harvard, Vancouver, ISO, and other styles
2

Thaweboon, Sroisiri, Ratchaporn Srichan, Supaporn Mala, and Boonyanit Thaweboon. "The Development of Artificial Saliva with Oral Wound Healing Property." In 2023 7th International Conference on Nanomaterials and Biomaterials & 2023 5th Asia Conference on Material and Manufacturing Technology. Trans Tech Publications Ltd, 2024. http://dx.doi.org/10.4028/p-wc6acn.

Full text
Abstract:
Wound healing is a normal biological mechanism of the body that helps to maintain the integrity of the tissue. In this process, fibroblasts play an important role in supporting wound healing by migrating to the injury site and laying down a new extracellular matrix. Oral wounds heal more rapidly than skin wounds due to the presence of saliva. However, a reduced salivary flow rate or hyposalivation is frequently found in some patients due to their systemic conditions or intake of various medications. In order to control or treat hyposalivation, the use of artificial saliva is recommended for these patients. Various agents were added to artificial saliva to improve its properties. The aim of the present study was to investigate the effect of artificial saliva containing vanillin on the wound healing of human gingival fibroblasts by inducing cell migration in vitro. Human gingival fibroblasts isolated from human gingiva were purchased from Scien Cell Research Laboratories, USA. The migratory ability of fibroblasts was performed on a confluent monolayer by the wound healing scratch assay. Artificial saliva with different concentrations of vanillin (0.12% to 4% w/v) was added and incubated for 24 h. Artificial saliva without vanillin was used as a control. The migration cells were fixed with 25% methanol and 0.2% toluidine blue. In vitro cell migration to the wound area was determined by photographing with an inverted microscope coupled to a digital camera (Nikon D 5100). In the presence of 0.25%, 0.5%, and 1% w/v vanillin-containing artificial saliva, human gingival fibroblasts had a significantly higher potential to migrate into the wound area than a control (p-value &lt;0.05). Data from this study provides the first scientific evidence to demonstrate the benefits of using artificial saliva containing vanillin to maintain healthy gums and accelerate oral wound healing. Rinsing the mouth with this artificial saliva is recommended as the most preferable method for moistening and lubricating the mouth and facilitating the healing of oral wounds in patients with hyposalivation.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Wound scratch assay' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography