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Dissertations / Theses on the topic 'Xanthomonas camXanthomonaspestris pv. campestris'

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Published: 27 July 2024

Last updated: 31 July 2025

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1

Garin, Tiffany. "Impact des compétitions inter-microbiennes médiées par le Système de Sécrétion de Type VI (T6SS) sur la dynamique de transmission du microbiote des graines aux plantules." Electronic Thesis or Diss., Angers, 2024. https://dune.univ-angers.fr/documents/dune18434.

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Les graines constituent la source d’inoculum initiale pour le microbiote des plantes. Lors de la germination, le relargage d’exsudats à proximité des graines déclenche une intense compétition microbienne qui joue un rôle moteur dans l’assemblage du microbiote des plantes. Ce travail de thèse vise à explorer le rôle de la compétition d'interférence médiée par le système de sécrétion de type VI (T6SS). Le T6SS d’une souche de Stenotrophomonas rhizophila, une espèce bactérienne transmise efficacement aux plantules, a été utilisé comme modèle d’étude. Ce T6SS est impliqué dans l’inhibition de la c

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DE, CRECY-LAGARD VALERIE. "Transport et metabolisme du fructose chez xanthomonas campestris pv. Campestris." Paris 7, 1991. http://www.theses.fr/1991PA077025.

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Xanthomonas campestris pv. Campestris est une bacterie phytopathogene qui secrete un polysaccharide, le xanthane. Le but de ce travail etait l'etude de la regulation du transport des sourcs de carbone chez x. Campestris. Les regulations trouvees chez e. Coli etaient-elles conservees ou existait-il d'autres mecanismes de regulation dans cette bacterie? chez e. Coli, deux systemes de regulation du transport des sources de carbone ont ete etudies en detail: le controle transcriptionnel par l'amp-cyclique associe a sa proteine receptrice cap; la regulation par le systeme des phosphotransferases de

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Drouin, François. "Caractérisation de l'intégron de Xanthomonas campestris pv badrii." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0019/MQ56399.pdf.

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Monteiro, Leila. "Produção de Substaâncias biotivas de Bacillus spp. contra Xanthomonas campestris pv. campestris." Universidade Federal de Pernambuco, 2002. https://repositorio.ufpe.br/handle/123456789/1304.

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Made available in DSpace on 2014-06-12T15:48:54Z (GMT). No. of bitstreams: 2 arquivo4388_1.pdf: 626983 bytes, checksum: 03353f7f9f78525329c28639adb99489 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2002<br>O gênero Bacillus é um dos mais utilizados no biocontrole de doenças de plantas. São microrganismos encontrados facilmente em solos e plantas, que formam esporos tolerantes ao calor e à dessecação, o que facilita sua comercialização e estoque. O principal mecanismo de ação desses organismos no controle de fitopatógenos é a produção

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Silva, Rafael Salomão da. "Bactérias de solos supressivos com atividade antimicrobiana sobre Xanthomonas campestris pv. campestris." Universidade Federal de Sergipe, 2016. https://ri.ufs.br/handle/riufs/3021.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>Xanthomonas campestris pv. campestris is a phytopathogenic bacterium, the causative agent of black rot in crucifers. For the control of plant pathogens diseases, there is the use of bacteria with activity antagonistic to the pathogen. Recent studies show that Bacillus species have on X. campestris a strong biological control. One of the mechanisms of this control is the production of secondary metabolites by these species. The objective of this work was to select bacteria X. campestris and antagonists to evaluate the antimi

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Sanders, Gina Mercia. "Detection of Xanthomonas Campestris PV. magniferaeindicae in mango plants." Diss., University of Pretoria, 1993. http://hdl.handle.net/2263/39792.

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The yearly losses incurred by bacterial blackspot disease are high. Often trees are asymptomatic, with the pathogen either in the resident phase or latent stage of infection. Detection of the pathogen in these asymptomatic trees is one of the most important means of controlling the disease. Isolates which consistently differed in virulence were isolated from symptomatic mango plants. These isolates could be categorised into four groups based upon differences in virulence. Monoclonal antibodies (mAbs) were successfully raised using separate and pooled isolates for immunisation. MAbsraised

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Pigatto, Gisele [UNESP]. "Irradiação UV em Xanthomonas campestris pv. campestris visando a produção da goma xantana." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/94867.

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Made available in DSpace on 2014-06-11T19:27:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-03-20Bitstream added on 2014-06-13T20:35:45Z : No. of bitstreams: 1 pigatto_g_me_sjrp.pdf: 376138 bytes, checksum: 0d3df3ba13a94adf6610c9416261a91c (MD5)<br>Xanthomonas campestris é uma bactéria fitopatogênica que causa a podridão negra no sistema vascular das plantas da família das cruciferaceaes. Produz um exopolissacarídeo denominado goma xantana, que possui propriedades reológicas únicas sendo utilizada amplamente como agente de suspensão, espessante, emulsionante e estabilizante. É apl

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Pigatto, Gisele. "Irradiação UV em Xanthomonas campestris pv. campestris visando a produção da goma xantana /." São José do Rio Preto : [s.n.], 2008. http://hdl.handle.net/11449/94867.

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Orientador: Pedro de Oliva Neto<br>Banca: Valéria Marta Gomes de Lima<br>Banca: Ivanise Guilherme Branco<br>Resumo: Xanthomonas campestris é uma bactéria fitopatogênica que causa a podridão negra no sistema vascular das plantas da família das cruciferaceaes. Produz um exopolissacarídeo denominado goma xantana, que possui propriedades reológicas únicas sendo utilizada amplamente como agente de suspensão, espessante, emulsionante e estabilizante. É aplicados em indústrias petrolíferas, alimentícias, farmacêuticas, mineração, têxtil, termoquímicas, tintas, cosméticos e produtos agropecuários. O B

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Silva, Fernanda Pereira da. "Caracterização biológica e molecular de um bacteriófago específico para Xanthomonas campestris pv. campestris." Universidade Federal de Viçosa, 2015. http://www.locus.ufv.br/handle/123456789/6502.

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Submitted by Amauri Alves (amauri.alves@ufv.br) on 2015-11-03T16:17:32Z No. of bitstreams: 1 texto completo.pdf: 584533 bytes, checksum: fd4ac14e1e677e1fdd9f3ee4f74281df (MD5)<br>Made available in DSpace on 2015-11-03T16:17:32Z (GMT). No. of bitstreams: 1 texto completo.pdf: 584533 bytes, checksum: fd4ac14e1e677e1fdd9f3ee4f74281df (MD5) Previous issue date: 2015-02-25<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Os vírus que infectam bactérias são denominados bacteriófagos, sendo também referidos como “fagos”. Os bacteriófagos que infectam bactérias fitopatogênicas,

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Wruck, Dulândula Silva Miguel. "Análises bioquímica, patogênica, sorológica e molecular de isolados de Xanthomonas campestris pv. campestris." Universidade Federal de Viçosa, 2001. http://www.locus.ufv.br/handle/123456789/11176.

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Submitted by Nathália Faria da Silva (nathaliafsilva.ufv@gmail.com) on 2017-07-11T14:11:33Z No. of bitstreams: 1 texto completo.pdf: 484351 bytes, checksum: 312994cb64033039a832571f86cbf2a3 (MD5)<br>Made available in DSpace on 2017-07-11T14:11:33Z (GMT). No. of bitstreams: 1 texto completo.pdf: 484351 bytes, checksum: 312994cb64033039a832571f86cbf2a3 (MD5) Previous issue date: 2001-07-06<br>Fundação de Amparo à Pesquisa do Estado de Minas Gerais<br>Trinta e três isolados de Xanthomonas campestris pv. campestris, obtidos de oito diferentes brássicas, provenientes de diferentes regiões do

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Rosseto, Flávio Rodolfo. "Caracterização bioquímica, biofísica e estrutural da principal endoglucanase secretada por Xanthomonas campestris pv. campestris ATCC33913." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-20092011-101408/.

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O esgotamento das fontes de combustíveis fósseis e questões ambientais têm gerado grandes preocupações para a sociedade, e alternativas sustentáveis e eficientes para solucionar e trazer inovações na produção de biocombustíveis estão cada vez mais próximas. O uso de biomassa pode ser uma vantajosa opção, mas para isso, é necessária a degradação das moléculas constituintes de sua parede celular a açúcares fermentáveis. A biotecnologia tem melhorado e aumentado as opções para suprir fontes sustentáveis e preservação do meio ambiente. A transformação de biomassa na escala industrial para produção

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Li, Pei-Zhen, and 李佩真. "Application of Xanthomonas campestris pv. campestris bla promoter." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/32617569531060590271.

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碩士<br>國立中興大學<br>分子生物學研究所<br>103<br>Xanthomonas campestris pv. campestris (Xcc) is a gram-negative bacterium that causes black rot in crucifers. Many strains of Xcc are β-lactam resistant via expressing β-lactamase (AmpC/Bla) constitutively. Previous studies showed that transcription from bla promoter is regulated by a global transcriptional regulator, Clp (cAMP receptor protein-like protein). Mutation in Clp changes constitutive expression of bla to an ampicillin-inducible manner. This study aimed to explore the possibility in application of Ap-inducibility in the clp-deficient mutant strain,

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王旭川. "Gene prediction and annotation in Xanthomonas campestris pv. campestris." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/53320871199889246880.

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碩士<br>國立清華大學<br>生命科學系<br>90<br>Abstract Xanthomonas campestris pv. campestis is a Gram-negative bacterium and one of the most important plant pathogens. It attacks cruciferous plants and causes worldwide agricultural loss. We annotate 575 contigs of X. campestris pv. campestis, which were sequenced by Genome Research Center at NYMU. We got 6327 and 3811 ORFs (open reading frame) predicted by Glimmer 2.0 and ORPHEUS. Then we performed BLAST search against Genbank nr (non-redundant) protein database and there were 5854 ORFs having similarity with protein sequences in

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HUANG, PEI-YI, and 黃佩儀. "Characterization of icd Gene of Xanthomonas Campestris pv. Campestris." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/638686.

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碩士<br>中臺科技大學<br>醫學檢驗生物技術系碩士班<br>105<br>The Gram-negative plant pathogen Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers, a disease that causes tremendous agricultural loss. The isocitrate dehydrogenase (IDH) is an enzyme that catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2 and NADPH or NADH, using NADP+ or NAD+ as a cofactor. In Xcc genome, the coding product of icd gene was annotated as a NADP+-dependent IDH. To characterize the function of Xcc icd gene, the icd mutant and its complementary strain were constructed pr

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Chen, Chih-Hua, and 陳智華. "Transcriptional Regulation by Clp in Xanthomonas campestris pv. campestris." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/56300758274356683357.

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博士<br>國立中興大學<br>分子生物學研究所<br>98<br>The Gram-negative plant pathogenic Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. It is capable of producing large amounts of an exopolysaccharide, xanthan gum, and secreting an array of extracellular enzymes, including proteases, pectinases, and endoglucanases, which have long been considered important virulence determinants. Production of these extracellular products is regulated by the global transcription factor Clp (cyclic AMP receptor protein-like protein, a homolog of the Escherichia coli CRP) and DSF who

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Shiau, Shuh-Lian, and 蕭澍濂. "Classification of Insertion Sequences in Xanthomonas campestris pv. campestris." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/66048286784555202974.

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Lo, Ta-Chun, and 羅大鈞. "Analysis of insertion sequences in Xanthomonas campestris pv. campestris." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/00898840262981581923.

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碩士<br>國立中興大學<br>分子生物學研究所<br>87<br>Xanthomonas campestris pv. campestris (Xcc) is the causing agent of black rot of crucifies. Using a sucrose-sensitive plasmid as a baiting plasmid, our laboratory previously isolated 34 plasmids containing IS insertions. Among these plasmids, thirty were characterized and were found to have IS476, IS1478, IS1479 and IS1480 insertion in the plasmids. All but one IS476 insertion mutant has target duplications of 4-6 bases at the insertion sites. An IS476 insertion mutant does not have any target site duplication. This study demonstrates that pXC11-11 contai

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Wang, Ching-Hua, and 王清華. "Characterization of hns Gene of Xanthomonas campestris pv. campestris." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/56591956311878025919.

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碩士<br>國立中興大學<br>分子生物學研究所<br>95<br>The gram-negative plant pathogenic Xanthomonas campestris pv. campestris causes black rot in crucifers. The virulence of this bacterium is mainly depending on its ability to produce large mounts of an exopolysaccharide (EPS, also called xanthan gum) and several extracellular enzymes, the virulence factors. It is known that the global transcription factor Clp (cAMP receptor protein-like protein) is involved in the production and secretion of these virulence factors. Using the consensus Clp-binding sequences (CBS) predicted in our laboratory for genome-wide s

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Wu, Hsung-Chi, and 吳順吉. "Transcriptional regulation of Xanthomonas campestris pv. campestris engXCA gene." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/83810288653140348308.

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碩士<br>國立中興大學<br>分子生物學研究所<br>90<br>The Gram-negative plant pathogenic Xanthomonas campestris pv. campestris causes black rot in crucifers, which results in large damage in agriculture. X. campestris. pv. campestris secretes a large mount of exopolysaccharide (EPS), xanthan gum, which is widely used as a stabilizing, viscosifying, emulsifying, thickening, and suspending agent. Therefore, it is of importance to study the biosynthesis of xanthan gum. Previously, by using Tn5(pfm)CmKm transposon mutagenesis, the gene in locus eps8 involved in xanthan synthesis and pathogenicity was cloned, seque

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Chou, Fan-Li, and 周芳莉. "Characterization of the gumD mutant from Xanthomonas campestris pv. campestris." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/67481305091835184718.

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碩士<br>國立中興大學<br>分子生物研究所<br>83<br>X. campestris pv. campestris 會引起十字花科蔬菜的黑腐病, 且會產生大量的胞外多醣 (exopoly-saccharide, 簡稱 EPS), 又稱黃原膠 ( xanthan gum)。為了研究黃原膠合成的基因, 選擇 p3DA01B 片段上的 pSR3, pRR4 與其下游片段的 pHN4, 利用標誌基因互換, 分離乾菌落突變株。結果只有送進 pSR3 片段所得到的菌落為乾菌落, 此突變株命名為 XKSR。在 pSR3 片段上帶有一 484 個胺基酸之 ORF, 相似於 GumD, 但與 GumD 有 5 個胺基酸序列不同的差異, 推測其功能為轉移第一個 glucose 至 lipid carrier 之 glucosyl transferase。此外, 在 pSR3 片段上帶有 gumE 基因產物 399 個胺基酸中的 304 個胺基酸。 ORF484 與 Rhizobium legusarum 胞外多醣調節

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Chang, Yu-Chia, and 詹于佳. "Charaterization of dsbA and dsbC of Xanthomonas campestris pv. campestris." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/d9rq39.

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碩士<br>中臺科技大學<br>醫學生物科技研究所<br>96<br>Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot in cruciferous plants. Xcc produces exopolysaccharide (EPS) and various extracellular enzymes, such as a-amylase, pectate lyase, endoglucanase and proteases, which are collectively essential for pathogenesis. Pathogenicity of Xcc is regulated by rpf gene cluster (for regulation of pathogenicity factors). Previous studies have shown that production of these virulence factors is positive regulated by rpf gene cluster. The amino acid sequence of RpfF has highly identity to enoyl-CoA hyd

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Chen, Jyu-Wei, та 陳巨威. "Regulation of β-lactamase of Xanthomonas campestris pv. campestris 17". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/65305099154396541785.

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碩士<br>國立中興大學<br>分子生物學研究所<br>97<br>Most of Xanthomonas camprstris pv. campestris(Xcc) have ability that they resisted beta-latam antibiotic. Most of Xcc destroyed beta-latam antibiotic by beta-lactamase. In genome of many germ negative bacteria, they existed ampR-bla system genes. When ampicillin existed in environment, the system is only induced and produced beta-lactamase. Although, Genome of Xc17 exist the system, but the system expressed beta-lactamase(XCC3039) continuously. Clp(cAMP receptor protein-like protein) is global transcription factor. Extracellular protein between P20H and P20H c

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Lu, Chun-Da, and 盧俊達. "Characterization of the fleN gene in Xanthomonas campestris pv. campestris." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/84854581535403713972.

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碩士<br>臺中健康暨管理學院<br>生物科技學系碩士班<br>93<br>Abstract Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot disease in cruciferous plants. This bacterium synthesizes great amounts of xanthan gum rendering the colonies mucoid. Xanthan gum has many industrial applications, such as oil drilling, food, agriculture and industry. Production of xanthan gum is also refuired for the virulence of this bacterium. To adapt to the diverse environmental changes, bacteria develop various responsive mechanisms. Bacteria are able to activate or switch off specific sets of genes as they face

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黃培瑄. "Transcriptional analysis of pilBA2A1CD cluster in xanthomonas campestris pv. campestris." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/56470531185800389731.

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碩士<br>國立中興大學<br>分子生物學研究所<br>90<br>AU56E, a clp mutant derived from Xanthomonas campestris pv. campestris strain 17, synthesizes no significant amounts of xanthan gum (an exopolysaccharide). In addition, this mutant is resistant to filamentous phage fLf due to the failure in phage adsorption. Furthermore, studies carried out in our laboratory have indicated that 1) fLf infection is through attachment to the type IV pilus, the primary receptor, 2) pilRSBA1A2CD gene cluster is essential for the normal biogenesis of pilus, and 3) biogenesis of the type IV pilus is regulated by Clp, a global tran

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WU, CHENG-YU, and 吳承宇. "Molecular characterization of secretion genes in xanthomonas campestris pv. campestris." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/77875113105871899036.

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Choy, Ka-Tim, and 蔡家添. "Physical Mapping and Gene Localization of Xanthomonas campestris pv. campestris." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/84563355524873210594.

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碩士<br>國立中興大學<br>分子生物研究所<br>82<br>Xanthomonas campestris pv. campestris is a Gram (-) bacterium which has a G+C content of about 65%. It is important in causing black rot in crucifers. In addition, it produces xanthan gum which has high viscosity and is widely used in industry. The purpose of this study was to construct a physical map of strain Xc17 and localize some genes in the physical map. Intact chromosomal DNA was prepared by embedding method, subjected to enzyme digestions with PacI,

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Chen, Day-Yu, and 陳戴瑜. "Mutational studies of xpsD gene of Xanthomonas campestris pv. campestris." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/56936596280293823056.

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碩士<br>國立中興大學<br>分子生物研究所<br>82<br>XpsD 為一外胞膜酯蛋白，是十字花科黑腐病菌分泌胞外酵素所必需的蛋白質之一。本研究利用一系列 xpsD 變異基因，探討 XpsD蛋白在胞外酵素分泌過程中可能的功能。以西方墨點法檢查 XpsD在細胞內的分佈位置，結果顯示 XpsD變異蛋白之分佈與野生株相同，免疫螢光標定實驗亦顯示，各變異蛋白均嵌入外胞膜，並有部分裸露於菌體外。除 pCD103及 pKU2 合成的 XpsD變異蛋白具分泌功能外，其餘皆喪失分泌功能。將變異基因以廣寄主性載體送入野生株 XC1701，利用固體培養基檢查澱粉及蛋白的分泌時發現在失去分泌功能的變異蛋白中,有部分呈現干擾分泌的現象，包括 pCD105，pKD2，pKdA6 及 pYL4 所合成的 XpsD 變異蛋白；pMH7，pKdPs 及 pKD20 所表現者，則無顯著干擾。利用澱粉抗體檢查XC1701轉接合菌株之澱粉分泌情形時， pMH7，pKdPs 及 pKD20表現

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Yu, Cheng-Tai, and 蔚承太. "Purification and Characterization of Xanthomonas campestris pv. campestris RNA Ploymerase." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/24965549804306715399.

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碩士<br>國立中興大學<br>分子生物研究所<br>82<br>In this study, we applied column chromatography and purified the RNA polymerase from the wild type strain of Xc.11. To achieve this purpose, 70.19 g of wet cell were collected and then broken by sonication, fractionation by 38% to 65% ammonium sulfate cut . The sample were applied into Heparin-Sepharose CL-6B column, and collected from bufferA with 0.2M, 0.35M and 0.5M KCl. The samples with enzyme activity eluted from 0.35M KCl were applied into Bio- Gel A

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Lee, Wen-Hung, and 李文弘. "Characterization of the ppk mutant of Xanthomonas campestris pv. campestris." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/63585401359034153133.

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碩士<br>亞洲大學<br>生物科技學系碩士班<br>93<br>Xanthomonas campestris pv. campestris (Xcc) is an important plant pathogen which causes black rot disease in cruciferous plants. The ppk gene encodes a polyphosphate kinase (PPK) responsible for the synthesis of inorganic polyphosphate (poly P). Poly P is a polymer of tens to hundreds of orthophosphate (Pi) residues linked by high-energy phosphoanhydride bonds , as in ATP. Poly P is ubiquitous , having been found in all organisms (archaea, bacteria, fungi, plants, insects, and animal cell). It is well known that poly P plays a role in the physiological adaptati

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Chang, Wen-Hui, and 張文輝. "Proteomic analysis of Clp-regulated Xanthomonas campestris pv. campestris genes." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/53777619398096985145.

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碩士<br>國立中興大學<br>分子生物學研究所<br>91<br>Xanthomonas campestris pv. campestris is a Gram-negative bacterium causing black-rot disease in crucifers. TC820 was a mutant derived from strain P20H by mini-Tn5 transposition at clp gene, encoding a protein homologous to cyclic AMP receptor protein (CRP), Clp (CRP-like protein). In this study, crude extracts prepared from P20H and TC820 were subjected to two-dimensional gel electrophoresis. In P20H and TC820, 201 and 148 spots were labeled, respectively, with 125 of the spots matched. Six of the proteins showing differences in amounts were analyzed by mass s

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Huang, Yi-ling, and 黃伊伶. "Transcriptional regulation of pmeA gene in Xanthomonas campestris pv. campestris." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/10700874467252550548.

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碩士<br>中臺科技大學<br>醫學生物科技研究所<br>98<br>Abstract Xanthomonas campestris pv. campestris (Xcc) is a Gram-negative plant-pathogenic bacterium that causes black rot in crucifers, resulting in tremendous losses in agriculture. This organism is capable of producing large amounts of exopolysaccharides and secreting several extracellular enzymes (such as protease, endoglucanase, mannanase, and pectinase), which have been considered to be important virulence determinants. The production of these extracellular products is coordinated by a cell-cell communication mechanism through diffusible signal factor (DS

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Shen, Jheng-Syun, and 沈政勳. "Functional study of fliF gene in Xanthomonas campestris pv. campestris." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/13818431905553598846.

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碩士<br>亞洲大學<br>生物科技學系碩士班<br>100<br>Xanthomonas campestris pv. campestris (Xcc) is a gram-negative bacterium. It can infect cruciferous plants and cause black rot disease. Xcc secrets various type of enzymes and polysaccharides which are essential for the bacterial virulence. Previous data from our lab have indicated that Xcc has a single polar flagellum. Biogenesis of the flagellum is regulated by the three-tier regulation of sigma factors RpoD, RpoN and FilA. A basal body composed of a flagellar type-III secretion system is essential for the secretion of the flagellin protein FliC. In other Gr

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Chen, Sho-Yi, and 陳脩易. "Characterization of the typA gene in Xanthomonas campestris pv. campestris." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/77286188697345000967.

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CHEN, DONG-TAI, and 陳東泰. "Nine filamentous phagesfrom Xanthomonas campestris pv. citri." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/31235591119072936691.

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碩士<br>國立中興大學<br>植物病理學研究所<br>79<br>Cf26、Cf29、Cf32、Cf86、Cf87、Cf89、Cf99、Cf117、Cf134等九個噬菌體分別從柑桔潰瘍病菌的XW26、XW29、XW32、XW86、XW87、XW89、XW99、XW117、XW134等潛溶菌株分離而得。此等噬菌體皆為絲狀, 大小在1250∼1550×8∼10nm 之間; 在XW47指示菌株的頂層培養基上, 形成直徑0.2∼1.5毫米的稍為混濁或混濁的溶菌斑; 每一溶菌斑含有約10 ∼10 PFU 的噬菌體顆粒; 以環狀的單股去氧核醣核酸組成它們的基因組 ,Cf117及其它8 個噬菌體基因組的分子量分別約為8.9kb 及7.9kb。此等噬菌體在70 ∼80℃ 10分鐘, pH值為2,15瓦的紫外燈距60公分下照射90∼150 秒, 或懸浮在50mM 的硫酸亞鐵溶液內1 小時, 以及丙酮、氯仿、乙醇、正丁醇、二甲苯等有機溶劑內5 分鐘, 則接近完全或完全不活化; 當懸浮於蒸餾水、Nutrient broth、GY broth、磷

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DONG, GUO-XIN, and 董國鑫. "Monoclonal antibody to xanthomonas campestris pv. cittri." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/64646671354209534191.

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Wu, Cheng-Der, and 吳承德. "Cloning and Characterization of hrcA from Xanthomonas campestris pv. campestris 17." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/81769534272027949086.

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碩士<br>國立中興大學<br>分子生物學研究所<br>88<br>The hrcA and its flanking sequences of Xanthomonas campestris pv. campestris 17 were cloned from genomic library using the DNA fragment carrying the 3'' end of hrcA sequence as a probe. After subcloning and sequencing the X. campestris hrcA and its upstream sequence were determined. Sequence analysis showed that the 1,053 bp hrcA can code for a protein of 350 amino acids with a predicted molecular mass of 38.3 kDa and PI of 5.87. Comparison of the amino acid sequences with those of other HrcA showed 36% identity to that of the HrcA of Caulobacter crescentus an

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Wang, Szu-Wen, and 王仕文. "Construction and Characterization of Xanthomonas campestris pv. campestris 17 hrcA Mutant." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/76465066687145192425.

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碩士<br>國立中興大學<br>分子生物學研究所<br>88<br>Plasmid pOKhrca carrying a DNA fragment span within the heat shock regulatory gene hrcA was constructed. After electroporation of pOKhrca into Xanthomonas campestris pv. campestris 17, several Kmr transformants were obtained, and one of them was verified to be a Xc17 hrcA mutant by Southern blotting. The growth rate of the hrcA mutant was slightly faster than that of Xc17 at 37℃. Northern blot analysis suggested that the expression of groESL in Xc17was mainly regulated by sigma factor s32, not by HrcA. Western blot analysis showed high levels of GroEL in h

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Chang, Chun-Yan, and 張君妍. "The study of regulatory protein, AlgR in Xanthomonas campestris pv. campestris." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/48546956713086174625.

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碩士<br>臺中健康暨管理學院<br>生物資訊研究所<br>94<br>Abstract Lf is a filamentous bacteriophage which specifically infects Xanthomonas campestris pv. campestris. A Lf resistant X. campestris strain was isolated by mini-Tn5 transposon insertional mutagenesis. The insertion site was mapped within algR, gene encoding for a transcriptional activator belonging to the microbial two component systems. Mutants of algR and algZ, the cognate sensor gene of algR, were reconstructed by insertional mutagenesis and the phenotypes of mutants were analyzed and summarized: (1) algR mutant is resistant to Lf infection and a

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39

Deng, Fu-Sheng, та 鄧福勝. "Translocation and expression of β-lactamase in Xanthomonas campestris pv. campestris". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/fjtjed.

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40

Chi-Kuo, Hu, and 胡智國. "The locational analysis of endogeneous IS1404s in Xanthomonas campestris pv. campestris." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/13455349563604571453.

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碩士<br>輔仁大學<br>生物學系<br>87<br>Three DNA fragments containing iso-IS1404s, named IS1404a, IS1404c and IS1404d, from Xanthomonas campestris pv. campestris Xcc1-1 genomic DNA were cloned and sequenced. Based on nucleotide sequence analysis of the flanking sequences of these iso-IS1404s, IS1404a is located in the ORFb of transposase gene of a new IS3 family member and IS476-like insertion sequence, ISA2. In addition to IS1404a, another new IS3 family member and IS1400-like insertion sequence, ISA1, also integrate the same ISA2. IS1404c is located in the transposase gene of an IS5 family insertion

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Wang, Tsai-Yuh, and 王彩諭. "Characterization of the Reconstituted RNA Polymerase from Xanthomonas campestris pv. campestris." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/42836205073532390667.

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碩士<br>國立中興大學<br>分子生物學研究所<br>87<br>Abstract After identified and characterized each subunits of Xc 11 RNA polymerase, the in vitro function of Xc 11 RNA polymerase by means of reconstituted RNA polymerase was subjected to be studied. The DNA fragment of each subunit were amplified by PCR with proof-reading Pfu or UlTma polymerase, and subcloned into protein expression vector pET-21. Concerning with the possibility of introducing mutation into DNA fragment after PCR amplification, we have sequenced the full length DNA of each RNA polymerase subunit genes in the protein expressi

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Shao, Yuan-Yuan, та 邵源源. "Expression and Regulation of Xanthomonas campestris pv. campestris α-Amylase Gene". Thesis, 1996. http://ndltd.ncl.edu.tw/handle/40911903765128769440.

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碩士<br>國立中興大學<br>分子生物研究所<br>84<br>Xanthomonas campestris pv. campestris (Xc)可分泌產生胞外酵素 α- amylase，將可溶性 starch 水解成 maltose、部分 maltotriose 以及微量之 glucose。為探討 Xc α-amy gene 啟動子之表現與調控，首先進行 Xc α-amy gene 上游核甘酸之定序。得到之核甘酸序列包含 α-amy gene coding region 之 255th bp 起至 5'' 端，全長共 1091 bps。分析結果顯示，α-amy 基因之調節區域可能包含 putative upstream activator-binding site ΩUAS, potential promoter -10/-35 cononical sequences, 2 段 inverted repeats ΩI 及 ΩII, 與 Shine-Dalgarno sequence (SD)。本研究中，利用報導基因 luxAB 反應

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Du, Shin-Chiao, and 杜歆喬. "Characterization of galE and galU Genes in Xanthomonas Campestris pv. Campestris." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/27039360000747718760.

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碩士<br>中臺科技大學<br>醫學檢驗生物技術系碩士班<br>101<br>UDP-galactose 4-epimerase (encoded by galE gene) and UTP-glucose-1-phosphate uridylyltransferase (encoded by galU gene) are enzymes responsible for the synthesis of UDP-galactose and UDP-glucose, substrates involved in the synthesis of different surface structures (lipopolysaccharide, exopolysaccharide, and capsular polysaccharide) in many bacteria. In a number of Gram-negative pathogens, galE and galU are known as important virulence factors. Xanthomonas campestris pv. campestris (Xcc) is a Gram-negative plant-pathogenic bacterium causing black rot in cr

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44

Chien, Hui-Chen Chang, and 張簡惠甄. "Characterization of the fliA, flgM, flhF genesin Xanthomonas campestris pv. campestris." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/40096826737703368468.

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碩士<br>亞洲大學<br>生物科技學系碩士班<br>95<br>Xanthomonas campestris pv. campestris (XCC) is a Gram negative, rod-shaped bacterium. It is the causative agent of black rot disease in cruciferous plants. The disease results in heavy loss in agriculture worldwide. Symptoms include marginal leaf chlorosis and darkening of vascular tissue, accompanied by extensive and necrosis. Full leaf yellowing, wilting, and necrosis occur as the disease advances. XCC has a single unipolar flagellum. From the completed genome sequence of XCC, more than 40 genes are predicted to be involved in the flagellar biogenesis. Resear

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Chien, Hsu-Ling, and 簡旭伶. "Regulation of pglA Gene of Plant Pathogenic Xanthomonas campestris pv. campestris." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/62414802313961724839.

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碩士<br>國立中興大學<br>分子生物學研究所<br>95<br>Abstract The Gram-negative plant pathogenic Xanthomonas campestris pv. campestris (Xcc) causes black rot in crucifers. This bacterium produces large mounts of an exopolysaccharide (EPS), xanthan gum, and an array of extracellular enzymes, including proteases, pectinases and cellulases. These substances are collectively required for pathogenicity. In addition to these substances, Avr (avirulence) proteins and the hrp genes encoded Type III secretory pathway are also essential for virulence. Regulation of the virulence factors in Xcc is not fully understood

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Wang, Yun-Ching, and 王雲慶. "Characterization of the Xanthomonas campestris pv. campestris lexA1 and lexA2 Regulons." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/68391760802020700583.

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碩士<br>國立中興大學<br>分子生物學研究所<br>94<br>Two lexA genes, named lexA1 and lexA2, had been identified in Xanthomonas campestris pv. campestris strain 17 (Xc17) in our previously studies. Computational search for genes possibly regulated by LexA1 and LexA2 proteins in the available genomic sequence of the X. campestris showed that the sequence in the upstream region of umuDC gene has an identical LexA1 binding motif. Southern blot analysis revealed that umuDC gene only exists in X. campestris ATCC33913 among the ten Xanthomonas strains studied. UmuD gene was demonstrated to be positively regulated by Le

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Chen, Ching-Jiun, and 陳俊磬. "Study on SOS response related genes in Xanthomonas campestris pv. campestris." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/74375043032962647410.

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碩士<br>國立中興大學<br>分子生物學研究所<br>94<br>It has been demonstrated the Xanthomonas campestris pv. campestris strain 17 (Xc17) contains two function and independent lexA genes. One of them (lexA1) constitutes a lexA-recA-recX operon and the second lexA (lexA2) belongs to a multiple gene cassette with the following gene order: lexA2-sulA2-dinP2-dnaE2. In the previous work, the Xc17 lexA1 regulon has been showed to be different form that E.coli and an analysis of the sequence of the recA upstream region revealed that the sequences required for LexA1 and LexA2 binding was not present. The aim of this stud

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Yang, Shu-hui, and 楊淑惠. "The study of regulatory protein, FleQ in Xanthomonas campestris pv. campestris." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/50995752607181959016.

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碩士<br>臺中健康暨管理學院<br>生物資訊研究所<br>92<br>To adapt to the diverse environmental changes, bacteria develop various methods. One of the methods is to use the alternative sigma factors to regulate gene expression. The 54 factor, encoded by rpoN, regulates genes involved in utilization of nitrogen and carbon sources, synthesis of flagellum and pilus, pathogenicity, RNA modification, and chemotaxis. Two functionally independent rpoN genes have been identified in Xanthomonas campestris pv. campestris (XCC). An interaction with an NtrC-like activator (also called enhancer-binding protein, EBP) is esse

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Chen, Po-hsuan, and 陳柏軒. "Functional analysis and application of vagA of Xanthomonas campestris pv. campestris." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/24932032101804625138.

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碩士<br>輔仁大學<br>生物學系<br>89<br>The study of the insertion sequence IS1404 of Xanthomonas campestris pv. campestris (XCC) leads to a discovery of a new gene, whose deduced amino acid sequence shows homology to VirD4 of Agrobacterium tumefaciens, TraG and TaxB of Escherichia coli, Cag5 of Helicobacter pylori, and RP293 of Rickettesia prowazekii. Those proteins contain NTP-binding Walker motif A and B, and the identity and similarity of the amino acid sequences range from 17% to 29% and 31% to 48%, respectively. The G+C content of the nucleotide sequences is from 33.3% to 63.6%. The gene knock-out

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50

Hsia, Tai Wei, and 夏代威. "Cloning and analysis of lon gene in Xanthomonas campestris pv campestris." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/25435732332034877119.

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碩士<br>國立中興大學<br>分子生物學研究所<br>89<br>Xanthomonas campestris pv. campestris is a gram-negative, rod-shaped and monotrichously flagellated plant pathogenic bacterium. The chromosome of X. campestris pv. campestris has a G + C content more then 60%. It produces copious amounts of xanthan gum, which has been implicated to associate with the virulence of the bacterium. Biosynthesis of xanthan gum involves multiple steps and a multitude of enzymes. Recently, our laboratory has localized the genes involved in xanthan synthesis at eight loci on the X. campestris pv. campestris 17 (Xc17) chromosome ma

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