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1

Lee, Ming-Yi. "XvERD15, an early-responsive gene to stress from Xerophyte viscosa." Master's thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/4287.

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Includes bibliographical references (leaves 44-54).<br>Genes that are upregulated in the early response to stress are not well understood. ERD15 (early-responsive to dehydration) in Arabidopsis and its homologues in various other plants have been shown to be upregulated within I hr post-exposure to dehydration and high salinity stress treatments. There is however limited literature on the functionality of ERD15. A cDNA showing homology to ERD15 was isolated from a library generated by low temperature stress treatment of Xerophyta viscosa and was subsequently named XvERD15.
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2

Thompson, Iris Lee. "Agricultural Systems in Babati : Zea mays and its alternatives." Thesis, Södertörn University College, School of Life Sciences, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-2660.

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<p>This paper aims to give an understanding on the aspects that influences the choice of cropsand agricultural methods in Babati, Tanzania. Drought is a reoccurring problem in this part ofTanzania, which affects a majority of the towns’ farmers. This fact is likely to affect thechoice of crop as well as the limitations when it comes to the choices in cultivation. But thechoice itself has implications on its surrounding and can be affected by the surrounding. Tounderstand the circumstances, a wider picture will be depicted and an evaluation of thegeography of Babati and on its climate condition
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3

Dace, Halford. "Metabolomics of desiccation tolerance in Xerophyta humilis." Master's thesis, University of Cape Town, 2014. http://hdl.handle.net/11427/9111.

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Includes bibliographical references<br>Resurrection plants are unique in the ability to survive near complete water loss in vegetative tissues without loss of viability. In order to do so, they employ multifaceted strategies which include structural adaptations, antioxidant and photoprotective mechanisms, and the accumulation of proteins and metabolites that stabilise macromolecules. A full understanding of the phenomenon of vegetative desiccation tolerance will require a systems view of these adaptations at the levels of the genome, the control of gene expression, and the control of metabolic
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4

Lyall, Rafe. "Regulation of desiccation tolerance in Xerophyta seedlings and leaves." Doctoral thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/22853.

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A small, diverse group of angiosperms known as resurrection plants display vegetative desiccation tolerance and can survive loss of up to 95% of cellular water, a feat only seen in the seeds and pollen of other angiosperms. Xerophyta humilis is a resurrection plant native to Southern Africa that has been the target of previous transcriptomic and proteomic studies into the mechanisms of plant desiccation tolerance. The aim of this study was to investigate the hypothesis that vegetative desiccation tolerance is derived from the networks that control desiccation tolerance in seeds and germinating
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5

Fan, Cynthia. "Characterisation of two desiccation-linked dehydrins from Xerophyta humilis." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/22723.

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In response to abiotic stresses, organisms throughout the plant kingdom, as well as microorganisms and micro-animals such as nematodes or tardigrades, have been observed to express Late Embryogenesis Abundant (LEA) proteins as protective mechanisms. However, despite two decades of research, little is understood about their physiological functions and this has led to extensive nomenclature, with a large amount of redundancy. The primary reason for this lack of insight into LEA protein functions is their highly hydrophilic and intrinsically disordered nature. Intrinsically disordered proteins (I
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6

Onyemata, Ezenwa James. "Structural and functional studies of XvPrx2, a type II peroxiredoxin protein from the resurrection plant xerophyta viscosa." Thesis, University of the Western Cape, 2012. http://hdl.handle.net/11394/5206.

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Philosophiae Doctor - PhD<br>XvPrx2 is a 1-Cys-containing member of the Prx5 subfamily of peroxiredoxins isolated from the resurrection plant Xerophyta viscosa. It is reported to be up-regulated during periods of desiccation and to protect nucleic acids and cellular proteins from oxidative damage through scavenging of reactive oxygen species, suggesting that it may play a role the desiccation tolerance of X. viscosa (Govender, 2006). Members of the Prx5 subfamily have previously been reported to occur as non-covalent homodimers associating across an A-type interface. PrxD from Populus tr
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7

Chireshe, Nyaradzo. "Stomatal control during dessication in the resurrection plant Xerophyta humilis." Bachelor's thesis, University of Cape Town, 2007. http://hdl.handle.net/11427/24964.

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Stomatal apertures on leaves of the resurrection plant Xerophyta humilis were monitored microscopically in order to characterize stomatal regulation during a dehydration time course. In addition, the effect of exogenous application of the stress hormone ABA on stomatal regulation was followed. X humilis stomatal regulation appears to be initially similar to that typical of desiccation sensitive plants, but differed in that stomata did not all close at once but at a slower rate to control the drying rate of the plant, this gave time for protection mechanisms to be laid down. The signal hormone
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8

Ellick, Tamaryn Lorean. "Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa." Master's thesis, University of Cape Town, 2012. http://hdl.handle.net/11427/10452.

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Includes bibliographical references.<br>The XvPSap1 promoter derived from Xerophyta viscosa has been demonstrated to be stress-inducible during dehydration in transgenic Nicotiana tabacum, black Mexican sweetcorn cells and Zea mays. To improve this promoter, for future applications in crop biotechnology, four shortened promoters, XvSap1D, E, F and G were generated by mutagenesis. The generated promoters had circa 50% reduction in size and contained the 5' proximal and 3' distal regions of the XvPsap1 promoter with the internal region removed. The shortened promoters displayed no significant se
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9

Ndima, Tozama Beauty. "Gene expression associated with drought tolerance in Xerophyta viscosa Baker." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/4309.

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Bibliography: leaves 89-100.<br>Herophyta viscosa (Baker) is a monocytyledonous resurrection plant that can tolerate extremes of dessication. Upon rewatering, it rehydrates completely and assumes its full physiological activities. Studies on changes in gene expression associated with dehydration stress tolerance were conducted. A cDNA library constructed from m RNA isolated from dehydrated (85%, 37% and 5% relative water content) X. viscosa leaves, was differently screened. Of the 192 randomly selected cDNAs screened, 30 showed higher expression levels when X. viscosa was dehydrated while 20 s
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10

Radermacher, Astrid Lillie. "Desiccation-driven senescence in the resurrection plant Xerophyta schlechteri (Baker) N.L. Menezes." Doctoral thesis, Faculty of Science, 2019. https://hdl.handle.net/11427/31708.

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Drought-induced senescence is a degenerative process that involves the degradation of cellular metabolites and photosynthetic pigments and uncontrolled dismantling of cellular membranes and organelles. Angiosperm resurrection plants display vegetative desiccation tolerance and avoid drought-induced senescence in most of their tissues. Developmentally older tissues, however, fail to recover during rehydration and ultimately senesce. Comparison of the desiccation-associated responses of older senescent tissues (ST) with non-senescent tissues (NST) will allow for understanding of mechanisms promo
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11

Waters, Robyn. "Molecular characterisation of the "LEAome" in the resurrection plant Xerophyta humilis (Baker)." Master's thesis, University of Cape Town, 2015. http://hdl.handle.net/11427/20012.

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Studies on resurrection plants and other anhydrobiotic organisms, have shown that Late Embryogenesis Abundant (LEA) proteins are expressed upon the onset of desiccation and are therefore inferred to be associated with the desiccation tolerance response. To date, despite some 25 years of research on these proteins, there is still very little understanding of the physiological function(s) of the majority of LEAs. This is because they lack tertiary structure in the hydrated state, making assigning of physiological roles difficult. This MSc study was undertaken to investigate the gene expression
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12

Gabier, Hawwa. "Label-free proteomic analysis of Xerophyta schlechteri leaf tissue under dehydration stress." Doctoral thesis, Faculty of Science, 2021. http://hdl.handle.net/11427/33783.

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Most higher plants cannot withstand severe water loss, except for a small group of angiosperms called resurrection plants. They can survive severe water loss without the loss of viability by employing mechanisms that aid them in desiccation tolerance. Desiccation tolerance in resurrection plants is a complex and multifaceted phenomenon and allows the plant to implement various strategies for survival. The focus of this study was a label-free proteomic analysis of Xerophyta schlechteri, a monocotyledonous and poikilochlorophyllous resurrection plant, in response to desiccation. The study invest
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13

Shen, Arthur Yen-Hsiang. "The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation." Doctoral thesis, University of Cape Town, 2014. http://hdl.handle.net/11427/13009.

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Includes bibliographical references.<br>In angiosperms, desiccation tolerance, a genetic trait that enables tissues to survive loss of more than 95% of cellular water is widely observed in the seeds, but is only found in the vegetative tissues of a small group of species known as the resurrection plants. Xerophyta humilis is a small resurrection plant indigenous to Southern Africa. In this study, the hypothesis that vegetative desiccation tolerance is derived from an adaptation of seed desiccation tolerance was tested by characterizing changes in the transcriptome of X. humilis leaves during d
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14

Neumann, Alexis Joy. "Characterisation of galactinol synthase II (XvGolSII) from the resurrection plant Xerophyta viscosa (Baker)." Master's thesis, University of Cape Town, 2013. http://hdl.handle.net/11427/6623.

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The monocotyledonous Xerophyta viscosa belongs to a unique group of angiosperms known as resurrection plants. These plants possess a number of unique characteristics which allow them to survive and cope for extended periods with extreme abiotic stresses such as dehydration and cold stress. It is therefore of great interest to understand and elucidate the various molecular mechanisms which are specifically regulated in response to abiotic stress by identifying genes and proteins which may contribute to abiotic stress tolerance. These genes could potentially be utilized in the development of cro
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15

Walford, Sally-Ann. "Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa." Master's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/20194.

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Bibliography: pages 128-163.<br>Prior to this study, a eDNA library of dehydrated Xerophyta viscosa was differentially screened and several genes were found to be upregulated during dehydration. One of these cDNAs was found to share a high degree of sequence identity with the ER-Iocated Hsp90 or Grp94 family of proteins (hereafter referred to as XVGrp94) and forms the basis of this work. The XVGrp94 eDNA was found to be truncated at the 5· terminus and a full length eDNA was isolated using SMART-RACE™ (§witching Mechanism gt 5' end of RNA Iranscript- Random ~mplification of Complementary .!;ri
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16

Ngubane, Nqobile A. C. "Cloning and characterisation of LEA1-EM genes in the resurrection plant, Xerophyta humilis." Master's thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/4312.

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Includes abstract.<br>Includes bibliographical references (leaves 75-86).<br>The presence and expressIon patterns of orthologues of LEA group 1 genes has been characterised in the resurrection plant, Xerophyta humilis. The group I LEAs (Em I and Em6) were first identified as proteins that were abundantly and specifically expressed during the desiccation and germination phase of angiosperm seed development. The group I LEA genes are characterised by the presence of one or more tandemly repeated 20-amino acid motifs that are particularly rich in Gly residues.
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17

Proctor, Jessica Diane. "The functional characterisation of the XhABFA transcription factor from the resurrection plant Xerophyta humilis." Master's thesis, Faculty of Science, 2021. http://hdl.handle.net/11427/32973.

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Vegetative desiccation tolerance (VDT), the ability to survive loss of up to 95% of cellular water in leaves and roots, is rare amongst vascular plants. However, the trait has evolved multiple times in a small, diverse group of angiosperms collectively known as “resurrection plants”. The physiological, morphological and metabolic changes that take place during VDT have been well characterised. However, in stark contrast, the underlying regulatory mechanisms that activate the VDT programme are not well understood. A widely held view is that VDT in resurrection plants may have arisen by a geneti
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18

Loffell, D. A. "Aspects of the physiology of the resurrection plant Xerophyta Humilis under different environmental conditions." Master's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/7724.

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Bibliography: leaves 92-100.<br>Xerophyta humilis is a resurrection monocot that is able to survive drying of its tissues to an air-dry state. in order to assess the alidity of laboratory-based experimentation, and the results obtained from such studies, a comparison of aspects of X. humilis physiology under different environmental conditions was undertaken. Analyses of chlorophyll fluorescence, gas exchange parameters, pigment concentrations and electrolyte leakage were performed on plants dehydrated under laboratory conditions differing with respect to light intensity ("high light" (ca. 1400
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19

Dennis, Timothy James. "The functional analysis of XhLEA3-2 - a LEA_4 from the resurrection plant, Xerophyta humilis." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/27094.

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Climate change is a pressing reality in the current era. Changing environmental conditions and limited water availability are associated with the loss of arable land in areas where farming has traditionally thrived. Thus, linked to climate change, is the risk of a global food shortage. Resurrection plants are phenomenal in that they are able to survive extended periods of drought in a state of anhydrobiosis and then resume full metabolism upon rehydration. These plants serve as models to scientists and genetic engineers who hope to replicate, to a degree, the 'resurrection phenomenon' in droug
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20

Chopera, Denis Rutendo. "Molecular characterization of XvlNO1, a myo-inositol 1-phosphate synthase gene from Xerophyta viscosa." Master's thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/4249.

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Includes bibliographical references.<br>Myo-inositol I-phosphate synthase (INO 1) catalyses the conversion of glucose-6-phosphate to myo-inositol I-phosphate, which is subsequently dephosphorylated to myo-inositol. Myo-inositol is a precursor for a number of important metabolites that include membrane components, storage molecules, phytohormones and a variety of osmoprotectants. Xerophyta viscosa Baker (Family Velloziaceae) is a monocotyledonous angiosperm which has the ability to resume full physiological function after desiccation. The full-length cDNA for INO1 from X viscosa was isolated us
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21

Myers, Marleen. "Molecular genetics of the floral response in Xerophta humilis." Master's thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/4306.

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22

Ginbot, Zekarias Gebremedhin. "Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis." Doctoral thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/10523.

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Studies on resurrection plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of XhLEA1-4S1 and XhLEA1-1S2, Group 1 LEA proteins from Xerophyta humilis, in order to shed light on their role in desiccation tolerance. Complementary DNA (cDNA) of these XhLEAs were cloned into bacterial expression vectors and the recombinant proteins expressed in E. coli. Antibodies wer
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23

Govender, Kershini. "Characterisation of XvPrx2 : a type II peroxiredoxin isolated from the resurrection plant Xerophyta viscosa (Baker)." Doctoral thesis, University of Cape Town, 2006. http://hdl.handle.net/11427/4268.

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Includes bibliographical references.<br>Knowledge of the biochemical and molecular mechanisms by which plants tolerate environmental stresses is necessary for genetic engineering approaches to improve crop performance. A unique feature of resurrection plants, such as Xerophyta viscosa, is their ability to cope with severe water loss of greater than 90%. A full-length cDNA library was synthesised from a cold stressed X viscosa plant. Sequencing and BLAST analysis revealed the identity of sixty genes. A type 2 peroxiredoxin (XvPrx2) was selected for further analyses as it was observed, by northe
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24

Sattar, Shakiera. "Molecular characterisation of the lateral organ boundaries gene (XvLOB) from the resurrection plant Xerophyta viscosa." Master's thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/11087.

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The aim of this study is to preliminary characterise XvLOB, a LOB domain containing gene, which has been isolated from a dehydration stress library in X. viscosa. In this study the gene was isolated and cloned, the DNA and amino acid sequences were analysed by in silico analysis, the recombinant XvLOB protein expressed, subcellular localisation examined and both the transcript and protein expression levels in response to dehydration was investigated.
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25

Milborrow, Evan. "Non-coding RNA networks regulating leaf vegetative desiccation tolerance in the resurrection plant Xerophyta humilis." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29437.

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Common to orthodox seeds, desiccation tolerance (DT) is exceedingly rare in the vegetative tissues of modern angiosperms, being limited to a small number of "resurrection plants". While the molecular mechanisms of DT, as well as the transcription factors regulating the seed and vegetative DT programmes, have been identified, very little is known with regards to the role of regulatory noncoding RNAs (ncRNAs). To investigate the presence and roles of possible ncRNA players, RNA-Seq was performed on desiccating Xerophyta humilis leaves and a bioinformatic pipeline assembled to identify the potent
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26

SILVA, Silvia Roberta Santos. "Adaptações morfoanatômicas de herbáceas em resposta a condições xéricas." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/18351.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-02-21T14:28:20Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) SILVIA_ROBERTA_SANTOS.pdf: 1500355 bytes, checksum: 1e02b5fad9ccef21b9e9af2f593d567c (MD5)<br>Made available in DSpace on 2017-02-21T14:28:20Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) SILVIA_ROBERTA_SANTOS.pdf: 1500355 bytes, checksum: 1e02b5fad9ccef21b9e9af2f593d567c (MD5) Previous issue date: 2016-02-29<br>CAPES<br>Espécies vegetais que ocorrem
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Rossetto, Estela de Sousa. "Aspectos celulares de folhas de Xerophyta plicata Spreng. (Velloziaceae), durante os processos de dessecação e revivescencia." [s.n.], 1992. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315426.

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Orientador: Mary Anne Heidi Dolder<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-07-15T21:22:13Z (GMT). No. of bitstreams: 1 Rossetto_EsteladeSousa_M.pdf: 4167185 bytes, checksum: 3b1892272dc3227a1ffb4e60b4d11e7b (MD5) Previous issue date: 1992<br>Resumo: Xerophyta plicata Spreng. (Velloziaceae) é uma Angiosperma brasileira tolerante à dessecação, que ocorre em afloramentos do RJ ao RN. Embora não perca as folhas nos períodos elas ficam amarelas, encolhidas, dobradas e torcidas. Quando a agua volta, as folhas se dist
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28

Walford, Sally-Ann. "Activation of seed-specific genes in leaves and roots of the desiccation tolerant plant, Xerophyta humilis." Doctoral thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/4349.

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Includes abstract.<br>Includes bibliographical references (leaves 131-169).<br>The ability of tissues to survive almost complete loss of cellular water is a trait found throughout the plant kingdom. While this desiccation tolerance is common in seeds of most angiosperms it is rare in their vegetative tissues. Xerophyta humilis (Bak.) Dur and Schintz belongs to a small group of resurrection angiosperms and it possesses the ability to withstand extreme desiccation of greater than 90% in both its seeds and vegetative tissues and return to active metabolism upon rehydration. We have tested the hyp
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29

Bajic, Jelena. "Exploring the longevity of dry Craterostigma wilmsii (homoiochlorophyllous) and Xerophyta humilis (poikolichlorophyllous) under simulated field conditions." Doctoral thesis, University of Cape Town, 2006. http://hdl.handle.net/11427/4240.

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Includes bibliographical references (p.143-164).<br>In the field C. wilmsii (homoiochlorophyllous) and X humilis (poikilochlorophyllous) plants do not experience drought longer than two and six months, respectively. In this study longevity of dry C. wilmsii and X. humilis plants was investigated for up to 10 months under four different sets of conditions. Some conditions resembled those under which the two species would experience drought in the field, but also included those that resemble the accelerated aging conditions used in seed studies.
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30

Cooper, Keren. "The effect of drying rate on the resurrection species Craterostigma wilmsii (homoiochlorophyllous) and Xerophyta humilis (poikilochlorophyllous)." Master's thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/6244.

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Bibliography: leaves 90-98.<br>Craterostigma wilmsii and Xerophyta humilis are angiosperm resurrection species that employ different strategies in dealing with desiccation and the related light stress. C. wilmsii is homoiochlorophyllous in that it retains its chlorophyll throughout dehydration, while X. humilis, a poikilochlorophyllous resurrection plant, breaks down its photosynthetic apparatus during drying.
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31

Bresler, Andries Petrus. "Molecular characterisation of XvVTC2, a gene coding for a GDP-L-galactose phosphorylase from Xerophyta viscosa." Master's thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/12376.

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Includes bibliographical references (leaves 100-108).<br>Climate change is predicted to have a negative impact on world food security in the next 40 years. Resurrection plants can withstand highly variable and harsh climatic conditions. This makes them ideal candidates to elucidate possible mechanisms which can be used to adapt crop plants to tolerate variable climatic conditions associated with climate change.
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32

Kamies, Rizqah. "A methodological investigation into the roots of the resurrection plant, Xerophyta viscosa, for further proteomic analyses." Master's thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/10955.

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In order to conduct proteomic analysis on the hydrated root tissues of the resurrection plant, Xerophyta viscosa Baker, aeroponically grown plant roots were subjected to various proteomic techniques. Three protein extraction methods were investigated, of which one method, was the most suited in isolating total protein from the root tissues of X. viscosa.
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33

Conrad, Nailah. "Characterization of XVEF and XvCaM : two calcium-binding proteins isolated from the resurrection plant Xerophyta viscosa." Master's thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/4251.

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Includes bibliographical references.<br>Xerophyta viscosa (Baker) is a resurrection plant with the ability to survive desiccation and rehydrate upon watering with minimal tissue damage. XVEF was isolated by differential screening of aX viscosa dehydration stress cDNA library. Reconstruction of the full length XVEF cDNA was conducted utilizing overlap extension PCR. XVEF codes for a putative calcium-binding protein and sequence analysis indicated that it has a 708 bp ORF corresponding to a protein with a molecular mass of26.95 kDa, has a conserved calcium-binding EF-hand motif, potential phosph
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34

Davis, Emily. "Molecular characterization of ALDRXv4, an aldose reductase orthologue isolated from xerophyta viscosa, in response to abiotic stress." Master's thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/4254.

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35

Tawouokam, Jean Bernard. "Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis." Master's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/4338.

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Bibliography: leaves 24-29.<br>Low temperature is one of the environmental factors that cause substantial crop losses in the world. Recent advances in the study of plants native from temperate regions, have established that cold acclimation is regulated at the gene expression leveL To study the function ofaXerophyta viscosa cold responsive protein that accumulates in plant cells under various environmental stresses, we have cloned the Xerophyta viscosa cold-responsive cDNA gene designed Xvcor, into a less complex host, Saccharomyces cerevisiae with the purpose of carrying out in vivo functiona
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36

Brown, Carly. "A comparison of some potential indicators of desiccation-tolerance in 2 Poikilochlorophyllous Xerophyta species and Homiochlorophyllous Craterostigma wilmsii." Bachelor's thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/24762.

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Three possible indicators of desiccation-tolerance in ang10sperms: sucrose accumulation and hexose sugar flux, an increase in hexokinase activity, and accumulation of LEA-like proteins, were investigated and compared during dehydration between two moncotyledonous poikilochlorophyllous resurrection angiosperm species; Xerophyta schlecterii and Xerophyta humilis, and a dicotyledonous homiochlorophyllous resurrection species, Craterostigma wilmsii. Comparisons were also made with Xerophyta viscosa, using data from Whittaker et al, (2001). Soluble sugar concentrations and hexokinase activities wer
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37

Abdalla, Kamal O. "Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa (Baker) and its response to dehydration stress." Doctoral thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/4233.

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Xerophyta viscosa Baker (family Velloziaceae) can survive extremes of dehydration (desiccation), down to 5% relative water content (RWC) and resumes full physiological activity within 80 h of rehydration. A thorough understanding of this phenomenon may provide further insight into possible mechanisms for improving drought tolerance in other plants. In this respect a comprehensive analysis of the nuclear proteome of this plant and its response to dehydration stress at 35% RWC was carried out. The RWC at 35% represents a distinct phase of the dehydration process where induction of late protectio
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38

Oduor, Okoth Richard. "Functional Analysis of the Novel Stress- Inducible XVPSAP promoter isolated from Xerophya Viscosa." Doctoral thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/4314.

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Gardner, Michael Jack. "Resources for the investigation of the desiccation tolerance mechanisms of the roots of Xerophyta humilis (Bak) Dur and Schinz." Master's thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/4263.

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Bibliography: leaves 119-150.<br>Although roots play an integral role in the sensing and amelioration of environmenatl stresses, there are no reports that specifically detail their involvement in the desiccation tolerance mechanisms of resurrection plants. Very lttle is known about even the genral anatomy and physiology of the roots of plants such as X. humilis, and almost nothing about the molecular responses that confer their ability to survive desiccation. This report details foundational studies of the functional anatomy and large-scale molecular responses of the roots during dehydrtion an
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Beckett, Megan. "Investigation into the role of volatile organic compounds, and abscisic in stomatal regulation, in the resurrection plant Xerophyta humilis." Master's thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/10102.

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The findings presented in this study give evidence for an ABA-mediated stomatal closure in response to initial dehydration of X. humilis, however, from 60% RWC onwards, stomata were observed to open again. This supplies further support for the hypothesis that resurrection plants actively lose water once protection is accumulated in order to minimize ROS activity and put a stasis on unregulated metabolism.
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McDonald, Zac Eliot. "Sucrose Phosphate Synthase activity and gene expression in relation to dehydration induced sucrose accumulation in the resurrection plan Xerophyta Humilis." Doctoral thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/4295.

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Marais, Saberi. "XvVHA-c``1- a novel stress responsive V-ATPase subunit c`` homologue isolated from the resurrection plant Xerophyta viscosa Baker." Master's thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/4291.

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43

Tshabuse, Freedom. "Characterization of membrane lipids and, Changes therein during desiccation and rehydration of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz." Doctoral thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/28121.

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Drought is the primary challenge facing agricultural productivity and food sustainability productivity in Africa and many parts of the world. Very few higher plants, including crop plants, can survive periods of extended water loss. However, a small group of angiosperms, termed resurrection plants are able to lose up to 95% of their cellular water content in vegetative tissue upon extended periods of water, remain in an air-dry for months to years and regain full metabolic activity in the same tissue upon re-watering. The aim of this study was to investigate the changes in acyl chain compositi
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Viana, Aline. "Morfologia e anatomia foliar de espécies de angiospermas com ocorrência nos campos de areais do bioma pampa." Universidade Estadual do Oeste do Paraná, 2018. http://tede.unioeste.br/handle/tede/3800.

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Submitted by Rosangela Silva (rosangela.silva3@unioeste.br) on 2018-07-06T12:01:53Z No. of bitstreams: 2 Aline Viana.pdf: 2056531 bytes, checksum: 5edb7c5297a393f052e5b8bc319a1bcf (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)<br>Made available in DSpace on 2018-07-06T12:01:53Z (GMT). No. of bitstreams: 2 Aline Viana.pdf: 2056531 bytes, checksum: 5edb7c5297a393f052e5b8bc319a1bcf (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-02-06<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>(Mo
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Mowla, Shaheen B. "Molecular characterization of XvPer1, a novel antioxidant enzyme from the resurrection plant Xerophyta viscosa, and AC3, a LEA-like protein from Arabidopsis thaliana." Thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/6728.

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By differential screening of a cDNA library of the resurrection plant Xerophyta viscosa, a cDNA termed XvPer I was isolated for its over-expression under dehydration stress. Analysis of the cDNA sequence indicated a hydrophilic protein of a predicted Mw of 24.2 kDa with high identity to plant l-Cys peroxiredoxins, a novel antioxidant enzyme. Southern blot analysis revealed that XvPer I was most probably a single copy gene. The polypeptide sequence had significant identities (-70%) with other recently identified plant I-Cys peroxiredoxins with an absolutely conserved active site (PVCTTE). The p
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Wiesner, Lubbe. "Isolation and characterisation of antiplasmodial compounds from Xerophyta species and the bioavailability, metabolic and efficacy evaluation of 9-0-acetylhydnocarpin in a mouse model." Doctoral thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/3304.

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Silva, Guilherme de Lira Sobral. "Morfometria e composiÃÃo quimÃca-bromatolÃgica da pornunÃa (Manihot sp.) em funÃÃo da densidade de plantio e adubaÃÃo fosfatada." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11509.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior<br>Nos Ãltimos anos, vÃrias pesquisas foram desenvolvidas na regiÃo SemiÃrida do Nordeste Brasileiro, no intuito de conhecer as potencialidades, tanto na produÃÃo como na qualidade de suas forragens. Como exemplo, a pornunÃa (Manihot sp.) na adiÃÃo de fonte de proteÃna no arraÃoamento de animais. Avaliou-se um modelo para a estimativa da Ãrea foliar, morfometria, composiÃÃo quÃmico-bromatolÃgica e mineral da pornunÃa (Manihot sp.) em funÃÃo da densidade de plantio e adubaÃÃo fosfatada. O experimento foi conduzido em sistema de sequei
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Maredza, Alice T. "Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene." Doctoral thesis, University of Cape Town, 2007. http://hdl.handle.net/11427/4292.

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Includes abstract.<br>Includes bibliographical references (leaves 170-209).<br>The Xerophyta viscosa aldose reductase cDNA (XvAld1) was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern blot analysis and DNA sequencing. The results revealed more than one copy of the gene with a complex banding pattern that was partially resolved by sequencing. The sequencing of PCRamplified genomic clones showed that the
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Peters, Shaun W. "XvGoIS, a galactinol synthase is transcriptionally upregulated under water deficit : the role of raffinosaccharides in abiotic stress tolerance in the resurrection plant Xerophyta viscosa (Baker)." Master's thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/4318.

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Includes bibliographical references (leaves 84-102).<br>As part of an ongoing project to genetically manipulate maize for increased abiotic stress tolerance, we have isolated and identified a number of genes from the resurrection plant Xerophyta viscosa (Baker) that are differentially expressed during water deficit. A cDNA was isolated from a library constructed from the leaves of water stressed X viscosa plants, which showed high identity to galactinol synthase (GoIS) enzymes from a variety of plant species. GolS is fundamental to the biosynthesis of raffinose family oligosachharides (RFOs),
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Pontes, Rodrigo Corrêa. "Avaliação fitogeográfica dos relictos xerófitos em afloramentos rochosos no campestre do divino, Santa Maria/RS." Universidade Federal de Santa Maria, 2017. http://repositorio.ufsm.br/handle/1/12162.

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The present study aims to identify and evaluate the areas of ecological refuges with xerophytic species, present in the locality of Campestre do Divino, Santo Antão district, Santa Maria municipality, central region of the State of Rio Grande do Sul, southern Brazil, through an environmental analysis of botanical, climatic, geological and geomorphological aspects. The research was developed through field data collection. Atmospheric variables were obtained through reading instruments. Geomorphological observations were performed together with the survey of the xerophytic species considered rel
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