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1

Law, Wai-yi. "Male midlife crisis as depicted in Chi Li's fiction Chi Li xiao shuo de nan xing zhong nian wei ji /." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42925721.

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Zhang, Xiao Chi [Verfasser], Jürgen [Gutachter] Margraf, and Robert [Gutachter] Kumsta. "Method matters : method effects in clinical psychological data / Xiao Chi Zhang ; Gutachter: Jürgen Margraf, Robert Kumsta ; Fakultät für Psychologie." Bochum : Ruhr-Universität Bochum, 2018. http://d-nb.info/1167504992/34.

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Zhang, Shifei. "Shanghai ai zheng zi zhu zu zhi yan jiu zu yuan can yu, she hui zhi chi he she hui xue xi de zeng quan xiao guo /." online access from Digital dissertation consortium, 2001. http://libweb.cityu.edu.hk/cgi-bin/er/db/ddcdiss.pl?3025931.

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Chen, Haochang. "Zhong yi yao zhi liao gong neng xing bian mi liao xiao wen xian ping gu /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b19986919a.pdf.

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5

Feng, Xiaoran. "Jin Yong wu xia xiao shuo zhong "wu" yu "xia" de cheng ji yu chuang xin = The inheritance and innovation of "wu" and "xia" in Jin Yong's novels /." click here to view the abstract and table of contents, 2000. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b15722648a.pdf.

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6

Chan, So Man. "Jin Yong wu xia xiao shuo lian zai ben (1962-1972) yu wen hua da ge ming /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202007%20CHAN.

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7

Lin, Xiaoru. "Cai xue xiao shuo "Jing hua yuan" = Flowers in the mirror as an erudite fiction /." click here to view the fulltext click here to view the abstract and table of contents, 2004. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisft.pl?pdf=b17981992f.pdf.

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Huang, Songliang. "Bian ge zhong de xue xiao ling dao Xianggang "ai guo xue xiao" de ge an yan jiu /." online access from Digital Dissertation Consortium, 2006. http://libweb.cityu.edu.hk/cgi-bin/er/db/ddcdiss.pl?3254535.

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9

Mu, Jian. "Zhuzi "Xiao xue" zhe xue yan jiu /." View abstract or full-text, 2003. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202003%20MU.

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Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2003.<br>Includes bibliographical references (leaves 140-145). Also available in electronic version. Access restricted to campus users.
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Cai, Xiao [Verfasser]. "The crystal of everyday life : The ethics of witness in Hou Hsiao-hsien's films / Xiao Cai." Mainz : Universitätsbibliothek Mainz, 2016. http://d-nb.info/1110918267/34.

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Chen, Fangyang. "Xiao chuan bing yin bing ji tan tao ji huan jie qi zhi ben de duo yuan xing /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b19986300a.pdf.

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12

Yan, Qiting. "Jiang nan xiao zhen : Zhongguo dang dai xiao shuo zhong de kong jian chang jing = Small towns in Jiangnan : geo-cultural setting in contemporary Chinese fiction /." click here to view the abstract and table of contents, 2001. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b17087661a.pdf.

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Wu, Yingni. "Zhang Dachun xiao shuo lun = Research on Zhang Da Chun's fiction /." click here to view the abstract and table of contents, 2000. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b15941218a.pdf.

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14

Riedl, Stefan. "Röntgenstrukturanalyse der Caspase-3-XIAP-BIR2, Caspase-7-XIAP-BIR2-Komplexe und der Procaspase-7." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964905426.

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Sun, Xiulin. "Dang dai Zhongguo de cun zhuang zhi li yu ji xiao fen xi /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?SOSC%202007%20SUN.

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Mok, Oi Lam. "Gu xiang xiang xiang : Mo Yan yu Li Rui xiao shuo yan jiu /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202007%20MOK.

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Tse, Dorothy Hiu Hung. "Xun mu : 90 nian dai da lu jia shi xiao shuo yan jiu /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202004%20TSE.

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Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2004.<br>Includes bibliographical references (leaves 153-166). Also available in electronic version. Access restricted to campus users.
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Guan, Qiyun. "Cong xian xiang xue jiao du tan tao da xue xue sheng ling xiu xiao yuan ke wai huo dong jing li de yi yi = The out-of-class experiences of university student leaders : a phenomenological approach /." click here to view the abstract and table of contents, 2002. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b17563380a.pdf.

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Lu, Yongsi. "Cong "xiao ben guan li tiao li" zheng lun kan quan li hua yu /." click here to view the abstract and table of contents, 2005. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b19816479a.pdf.

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Mak, Yan Yan. "Cong xiao shuo dao dian ying : lun "Qing cheng zhi lian" yu "Ban sheng yuan" /." View Abstract or Full-Text, 2002. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202002%20MAK.

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Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2002.<br>Includes bibliographical references (leaves 276-289). Also available in electronic version. Access restricted to campus users.
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Li, Yu. "Zou xiang ji xiao she hui : Zhongguo cheng zhen de dai ji liu dong (1966-2003) /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?SOSC%202006%20LIY.

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Lü, Yongjia. "Xi Xi xiao shuo zhong de "cheng shi kong jian" = "Urban space" in Xi Xi's novels /." click here to view the fulltext click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisft.pl?pdf=b2020842xf.pdf.

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Zhu, Lin. "Xian dai Han yu zuo ge jie gou /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202007%20ZHU.

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Mai, Shujian. "Wan Qing Shanghai xiao shuo de cheng shi shu xie = City writing in late Qing Shanghai fictions /." click here to view the fulltext click here to view the abstract and table of contents, 2004. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisft.pl?pdf=b17982091f.pdf.

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Amin, Md Ashraful. "Gabor wavelets for human biometrics = Gaibo xiao bo zai ren ti shi bie zhong de ying yong /." access full-text access abstract and table of contents, 2009. http://libweb.cityu.edu.hk/cgi-bin/ezdb/thesis.pl?phd-ee-b23749489f.pdf.

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Thesis (Ph.D.)--City University of Hong Kong, 2009.<br>"Submitted to the Department of Electronic Engineering in partial fulfillment of the requirements for the degree of Doctor of Philosophy." Includes bibliographical references.
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Zhuang, Songhui. "Bi zheng de gu wen xian zheng li /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b1998702xa.pdf.

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Zheng, Ruiqin. "Xi Xi "Tong hua xie zhen" xiao shuo yan jiu = Research on the fairy realism in Xi Xi's writings /." click here to view the abstract and table of contents, 2000. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b15722624a.pdf.

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Tang, Siu Wa. "Xian dai xing de you ling : Wang Xiaobo yan jiu /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202006%20TANG.

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Li, Ke. "Shi du cha ju yu xi tong you hua Zhongguo xian dai hua jin cheng zhong de qu yu jing ji /." Beijing : Zhongguo she hui ke xue chu ban she, 2000.

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30

Chan, Kung Fong. "Cong jiao yu zhong "gai zao" nü xing : yi "Guangdong Sheng li di yi nü zi shi fan xue xiao" wei ge an yan jiu (1907-1938) /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202004%20CHAN.

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Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2004.<br>Includes bibliographical references (leaves 323-342). Also available in electronic version. Access restricted to campus users.
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Li, Wen. "Shui shang fu sheng : yi ge bian yuan qun ti zai xian dai Zhongguo Guangdong Xijiang de xing cheng /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202006%20LIW.

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Li, Kangdi, and 李康迪. "An investigative analysis on Hong Kong international school students' willingness to communicate in Chinese : in a Hong Kong ESF international school = Xianggang guo ji xue xiao xue sheng Han yu jiao ji yi yuan diao cha yu fen xi : yi Xianggang mou Ying ji guo ji xue xiao wei li." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/209644.

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本研究探究了香港某所國際學校中漢語作為第二語言學習者的中文交際意願。研究對象主要是香港某英基國際學校的170名漢語學習者;主要採用定量的研究方法,通過研究者的課堂觀課和學生的“自我報告”(Self-report)來搜集數據,通過五個量表(背景變量量表、心理變量量表和課堂內外漢語交際意願量表)來實現研究;研究的內容包括學生們課堂內、外漢語交際意願的總體水平以及可能存在的差異,並總結前人研究發現,在新的研究背景下對一些可能存在影響關係的背景變量(年齡、性別、漢語水平、學習漢語的社會支持、母語、性格、對待學習漢語的態度)和心理變量(學習漢語時的焦慮、動機、自我覺察的交際能力)進行數據分析并驗證假設,通過SPSS19.0來付諸實現;最終就如何提高學生的課堂內、外漢語交際意願提出教學建議。 本次研究的主要研究發現有:(1)該國際學校漢語學習者的總體漢語交際意願水平偏低,介於“較低交際意願”和“中度交際意願”之間,而且課內漢語交際意願略高於課外漢語交際意願。(2)總體來看,男生和女生之間不存在漢語交際意願上的顯著差異,但在課內,女生比男生更容易不懂就問、更關注語言知識的細節;男生比女生更願意作為發言代表來公開表達自己觀點;在課外,男生比女生更願意在校園裡與陌生人說漢語,更願意和朋友們或者陌生人上網用漢語交流。(3)預科項目(DP)和中學項目(MYP)的學生群體間不存在漢語交際意願的顯著差異。但在課堂上,DP學生比MYP的學生更願意進行公開性的漢語表達,更願意深入探討相關問題,更願意進行一些總結概括能力較強的發言。(4)DP年級學生的漢語水平和交際意願呈顯著正相關,即漢語水平高越高,課內、課外和總體的漢語交際意願越高,漢語水平越低,課內、課外和總體的漢語交際意願越低。(5)在用漢語進行“主動性發言”上的意願,漢語水平高的群體明顯高於漢語水平低的群體,說明高水平漢語學習者更願意掌握交際的主動權。(6)學習漢語社會支持較多的群體表現出明顯更高的漢語交際意願,呈顯著正相關。(7)以廣東話作為母語的學生比以英語作為母語的學生具有更高的漢語交際意願,這種差異性在課堂外比課堂內體現得更為明顯。(8)性格較外向的學生比性格較內向的學生具有更高的課內漢語交際意願。(9)喜歡學漢語的學生比不喜歡學漢語的學生具有更高的課內、課外和總體漢語交際意願。(10)總體來看,學生學習漢語的動機和自我覺察的漢語交際能力都偏高,且兩者與課內、課外和總體漢語交際意願均呈十分顯著正相關,其中動機與課外方面相關性略大,而自我覺察的交際能力與課內方面相關性略大。學生的總體焦慮水平偏低,與課內、課外和總體交際意願均呈十分顯著負相關,與課內方面相關性略大課外方面。無論是課內還是課外漢語交際意願,“自我覺察的交際能力”對漢語交際意願來說是預測性最強的心理變量。 This research investigated Chinese as second language (CSL) learner’s willingness to communicate (WTC) both inside and outside classroom in one of international schools, Hong Kong. The research objects are 170 Chinese Language B learners from an English School Foundation (ESF) international school. The main methodology adopted here was quantitative method. Classroom observations by researcher and self-reports by students were firstly done to collect background data, and then five scales were employed to do the main investigation, including a WTC Inside–the-classroom Scales (WTCIS), a WTC Outside-the-classroom Scale (WTCOS), a Language Anxiety Scale (LAS), a Motivation Scale (MS) and a Self-perceived Communicative Competence Scale (SCCS). The research objectives were to explore the general situation of CSL learners’ WTC, and the specific characteristics as well as possible differences of their WTC inside and outside the classroom; then to further examine the possible affecting variables from both background and psychological perspectives including gender, age, Chinese language proficiency, mother tongue, social support, personality and attitudes towards learning Chinese, which were regarded as background variables, and language anxiety, motivation and self-perceived communicative competence, which were regarded as psychological variables. After that, data was processed and analyzed by SPSS 19.0 for hypothesis verification. Finally, pedagogical implications on how to improve CSL learners’ WTC both inside and outside classroom were generalized from the study.   The major findings showed that: (1) the general WTC of CSL learners from this school were slightly on the low side, between the Lower and Middle level, and the WTC inside the classroom was comparatively higher than outside of classroom. (2) On overall WTC, there was no significant difference between male and female students. But inside the classroom, girls were more likely to ask questions and pay more attention on language details than boys, while boy were more active to be the presenters and voice out in public. Outside the classroom, boys were more willing to communicate with strangers or chat online with friends in Chinese. (3) No significant WTC difference was found between Diploma Program (DP) and Middle Years Program (MYP), but DP CSL learners showed more willingness to make public speech, to be more of an inquirer in problems, and to do more generalization in speaking. (4) Among DP group, significant positive correlation was found between language proficiency and WTC, suggesting that the more proficient CSL learners showed higher WTC, and vice versa. (5) The more proficient group were more willing to speak Chinese voluntarily than the less proficient ones, implying that the more competent language learners are more likely to the take the initiative in communication. (6) CSL learners with more social supports to learn Chinese showed higher WTC, and a significant positive correlation was found here. (7) Students with Cantonese as mother tongue had higher WTC than their English as mothertougue counterparts, and this difference was more obviously outside of classroom. (8) Extroverted students showed significantly higher WTC inside of the classroom than the introverted. (9) Students who liked learning Chinese demonstrated higher WTC than those who didn’t. (10) Students’ motivation to learn Chinese and their SPCC were both on the high side, and both correlated positively on WTC, with motivation correlated more closely on outside WTC while SPCC more on inside WTC. Students showed slight lower anxiety level in speaking Chinese. Anxiety had significant negative correlation with WTC, and it mattered more inside than outside of classroom. No matter for WTC inside or outside, SPCC was the most predictive variable among the three.<br>published_or_final_version<br>Education<br>Master<br>Master of Education
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Kam, Kin-kei. "The social awareness of Shen Congwen's (1902-1988) Xiangxi fictions / Shen Congwen xiang xi xiao shuo zhong de she hui yi shi." Hong Kong : University of Hong Kong, 1999. http://sunzi.lib.hku.hk:8888/cgi-bin/hkuto%5Ftoc%5Fpdf?B22752833.

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Law, Shun Man. "Lun Mou Zongsan xian sheng de fo jiao yuan jiao guan nian /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202007%20LAW.

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Chen, Yingyi. "Zhong yi yao zhi liao bao long de wen xian zong shu /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b19986749a.pdf.

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Lu, Pei'er. "Xu shu "jiu qi" : Xianggang xiao shuo zhong de shi jian yu xu shi = Narrating "1997" : time and narrative in Hong Kong novels /." click here to view the fulltext click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisft.pl?pdf=b19843926f.pdf.

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Liang, Yaoguo. "Shi zhen bian zheng lun zhi gui lü de wen xian yan jiu /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b19987122a.pdf.

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Yu, Xiulan. "Zhongguo jiao yu de cheng xiang cha yi yi zhong wen hua zai sheng chan xian xiang de fen xi = Zhongguo jiaoyu de chengxiang chayi /." Beijing : Jiao yu ke xue chu ban she, 2004.

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Wong, Kam-lai. "A study of primary teachers' opinions of the teachers' handbooks for Chinese language textbooks Xiao xue jiao shi dui "Zhongguo yu wen ke jiao shi yong shu" de yi jian diao cha yan jiu /." Click to view the E-thesis via HKUTO, 1998. http://sunzi.lib.hku.hk/hkuto/record/B31960522.

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Lin, Jiaying. "Pian zhang xian jie de yu yong fen xi = Pragmatic analysis of discourse cohesion /." click here to view the fulltext click here to view the abstract and table of contents, 2004. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisft.pl?pdf=b17982273f.pdf.

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Tsang, Anthony Hiu King. "S-nitrosylation of XIAP compromises its protective function : implications to the pathogenesis of Parkinson's disease /." View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?BICH%202009%20TSANG.

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Luo, Meng. "Chong du "Hai shang hua lie zhuan" : kong jian, xu shi yu xian shi zhu yi /." View abstract or full-text, 2008. http://library.ust.hk/cgi/db/thesis.pl?HUMA%202008%20LUO.

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Seabra, Eduardo Jos? Guerra. "Avalia??o in vitro da atividade antimicrobiana de diferentes solu??es a base de hidr?xido de c?lcio." Universidade Federal do Rio Grande do Norte, 2009. http://repositorio.ufrn.br:8080/jspui/handle/123456789/13137.

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Made available in DSpace on 2014-12-17T14:13:26Z (GMT). No. of bitstreams: 1 EduardoJGS.pdf: 119973 bytes, checksum: dbdb322fee12ba3edd9d72e5446d9c91 (MD5) Previous issue date: 2009-09-23<br>The science of Dentistry wishes obtains the ideal solution for the dental plaque chemical control. This research evaluated antimicrobial action capacity in calcium hydroxide and tergentol various solutions starting for the CHD 20, a root canals irrigating solution with a reason of 80% calcium hydroxide saturated solution and 20% tergentol detergent with the aim of evaluate this drug mouth rinse indication with prevention or combat objective for dental caries and periodontal diseases. Antibiogram disks and biofilm tests were accomplished for the microorganisms: Streptococcus mutans, Streptococcus sanguis, Streptococcus sobrinus and Lactobacillus casei. Different reasons of detergent for the calcium hydroxide saturated solution, tergentol and distillated water solution, 0,12% clorhexydine digluconate solution was positive control and distillated water was negative control. The results showed better performance of clorhexydine in relation to calcium hydroxide directing to not accept this (CHD20) as mouth rinse solution<br>Na busca pela ci?ncia odontol?gica de se chegar ao composto que possa ser considerado o agente ideal para o controle qu?mico do biofilme dental foi idealizado este estudo. Avaliou-se a capacidade antimicrobiana in vitro de diferentes solu??es a base de hidr?xido de c?lcio e tergentol partindo em princ?pio do HCT 20, solu??o irrigadora dos canais radiculares composta por 80% da solu??o saturada de hidr?xido de c?lcio (?gua de cal) e 20% do detergente tergentol buscando verificar sua poss?vel indica??o como solu??o para bochechos, visando preven??o ou combate a doen?as como c?rie dent?ria e doen?a periodontal. No laborat?rio de microbiologia do Departamento de Odontologia da UFRN, foram realizados testes em discos de antibiograma para os microrganismos: Streptococcus mutans, Streptococcus sanguis, Streptococcus sobrinus e Lactobacillus casei. Bem como em bact?rias formadoras de biofilme para os mesmos, ? exce??o do L. casei. Estipulou-se diferentes concentra??es de tergentol para a ?gua de cal, al?m do tergentol em ?gua destilada, usou-se digluconato de clorexidina a 0,12% como controle positivo e ?gua destilada como controle negativo. Os resultados mostraram desempenho inferior das solu??es a base de Ca(OH)2 em rela??o ? clorexidina frente a estes microrganismos e ? metodologia empregada, direcionando pois, para a n?o indica??o do uso do HCT 20 como colut?rio bucal
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Chen, Dejin. "Zhongguo xian dai xiang tu san wen yan jiu = A study of modern Chinese nativist prose /." click here to view the abstract and table of contents, 2002. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b17087557a.pdf.

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Zhang, Jinling. "Nü zhen zi de xian dai yao xue yan jiu ji qi lin chuang ying yong /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b19986968a.pdf.

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Gu, Donghui. "Shanghai xia gang zhi gong yan jiu she hui zhi chi xi tong, ge ren hui ying yu zai jiu ye = A study of the unemployed in Shanghai : social support systems, individual responses & reemployment /." online access from Digital dissertation consortium, 2000. http://libweb.cityu.edu.hk/cgi-bin/er/db/ddcdiss.pl?9984698.

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Ng, Wai-yip. "Impact of climatic change during little ice age on agricultural development in north China, 1600-1650 Xiao bing qi qi hou bian qian yu Hua bei nong ye fa zhan : 1600-1650 nian jian de guan cha /." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43209397.

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Ma, Yihua. "Zhong yi yao zhi liao tong jing (yuan fa xing tong jing) de wen xian yan jiu /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b1998747xa.pdf.

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Liang, Ruhai. "Pi wei gong neng yu pi fu bing guan xi de gu jin wen xian yan jiu /." click here to view the abstract and table of contents, 2006. http://net3.hkbu.edu.hk/~libres/cgi-bin/thesisab.pl?pdf=b20009628a.pdf.

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Martins, Aline de Oliveira. "Avalia??o de m?todos alternativos utilizando o corante lissamina green B (C.I. 44090) para a determina??o de di?xido de cloro em processos de tratamento de ?gua." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2007. http://tede2.pucrs.br/tede2/handle/tede/3288.

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Made available in DSpace on 2015-04-14T13:59:18Z (GMT). No. of bitstreams: 1 393354.pdf: 726579 bytes, checksum: d7c29233cb1df65bec37a23c4be59ca5 (MD5) Previous issue date: 2007-03-28<br>Atualmente ? crescente o uso de desinfetantes alternativos ao cloro no tratamento de ?guas devido ? gera??o de subprodutos indesej?veis, como os trihalometanos e os ?cidos haloac?ticos no processo de desinfec??o convencional. O di?xido de cloro (ClO2) vem se destacando nos ?ltimos anos como desinfetante auxiliar nas esta??es de tratamento de ?gua (ETAs) devido as suas caracter?sticas qu?micas e de sua gera??o in situ. Entretanto, o uso desse desinfetante pode gerar subprodutos dentre os quais se destaca o ?on clorito (ClO2-), potencialmente prejudicial ? sa?de humana, possuindo limite m?ximo de toler?ncia de 0,2 mg L-1 estabelecido pela legisla??o brasileira. Neste contexto, ? importante a avalia??o e o aprimoramento de t?cnicas anal?ticas sens?veis, precisas e adaptadas ? complexidade de diferentes amostras. Recentemente, o m?todo EPA 327.0 foi proposto especificamente para an?lise de ClO2 e ClO2- em ?guas tratadas. Esse m?todo espectrofotom?trico utiliza uma solu??o colorida ("lambda"max = 633 nm) contendo Horseradish Peroxidase, enzima que catalisa a rea??o de convers?o do ?on clorito em di?xido de cloro, e o corante Lissamine Green B que reage rapidamente e seletivamente com o ClO2 reduzindo proporcionalmente a absor??o da solu??o na regi?o do vis?vel. A quantifica??o do ClO2 ? feita por calibra??o externa e a concentra??o do clorito ? obtida por diferen?a, atrav?s da an?lise de uma segunda al?quota na qual o di?xido de cloro ? previamente retirado por borbulhamento com um g?s inerte. Neste contexto, os objetivos desse trabalho s?o implantar o m?todo EPA 327.0 para an?lise de di?xido de cloro e do ?on clorito ao longo do processo de tratamento, bem como otimiz?-lo para an?lises de ClO2 em solu??es concentradas e como contaminante em solu??es padr?o do ?on clorito. Os resultados obtidos indicaram que o m?todo EPA 327.0 ? adequado para a an?lise de ClO2 em ?gua pot?vel, por?m n?o se verificou sua presen?a nesse tipo de amostras. Por outro lado, o m?todo n?o ? satisfat?rio para a determina??o do ?on clorito nessas amostras apresentando um limite de quantifica??o elevado (0,2 mg L-1). N?o ? recomendada a utiliza??o desse m?todo para o acompanhamento das concentra??es dos analitos durante o processo de tratamento devido ? variabilidade nas caracter?sticas (cor, turbidez, s?lidos dissolvidos, entre outras) da ?gua gerando artefatos na an?lise espectrofotom?trica que n?o podem ser corrigidos por procedimentos usuais. Em fun??o dessas limita??es, o m?todo foi otimizado com a elimina??o da enzima e com modifica??es nos procedimentos de an?lise (concentra??o do corante, ado??o de medida volum?trica e mudan?a na ordem da adi??o de reagentes e amostras, dilui??es, tempo de rea??o, caminho ?ptico, entre outros). Com as altera??es propostas foi poss?vel quantificar o di?xido de cloro tanto em elevadas concentra??es (> 100 mg L-1) bem como sua presen?a como contaminante (< 4,5 mg L-1) em solu??es concentradas (1000 mg L-1) do ?on clorito. Assim, desenvolveu-se um m?todo preciso e r?pido que permite adequar par?metros anal?ticos como concentra??o dos reagentes, volume de amostra e tempo de rea??o, possibilitando a an?lise de di?xido de cloro em diferentes matrizes.
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