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Journal articles on the topic 'Xylose utilization'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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1

Hector, Ronald E., Jeffrey A. Mertens, and Nancy N. Nichols. "Identification of Mutations Responsible for Improved Xylose Utilization in an Adapted Xylose Isomerase Expressing Saccharomyces cerevisiae Strain." Fermentation 8, no. 12 (2022): 669. http://dx.doi.org/10.3390/fermentation8120669.

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Economic conversion of biomass to biofuels and chemicals requires efficient and complete utilization of xylose. Saccharomyces cerevisiae strains engineered for xylose utilization are still considerably limited in their overall ability to metabolize xylose. In this study, we identified causative mutations resulting in improved xylose fermentation of an adapted S. cerevisiae strain expressing codon-optimized xylose isomerase and xylulokinase genes from the rumen bacterium Prevotella ruminicola. Genome sequencing identified single-nucleotide polymorphisms in seven open reading frames. Tetrad anal
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2

Kim, Jae-Han, Sharon P. Shoemaker, and David A. Mills. "Relaxed control of sugar utilization in Lactobacillus brevis." Microbiology 155, no. 4 (2009): 1351–59. http://dx.doi.org/10.1099/mic.0.024653-0.

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Prioritization of sugar consumption is a common theme in bacterial growth and a problem for complete utilization of five and six carbon sugars derived from lignocellulose. Growth studies show that Lactobacillus brevis simultaneously consumes numerous carbon sources and appears to lack normal hierarchical control of carbohydrate utilization. Analysis of several independent L. brevis isolates indicated that co-utilization of xylose and glucose is a common trait for this species. Moreover, carbohydrates that can be used as a single carbon source are simultaneously utilized with glucose. Analysis
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3

Panchal, Chandra J., Lynda Bast, Inge Russell, and Graham G. Stewart. "Repression of xylose utilization by glucose in xylose-fermenting yeasts." Canadian Journal of Microbiology 34, no. 12 (1988): 1316–20. http://dx.doi.org/10.1139/m88-230.

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The xylose-fermenting yeasts Pichia stipitis, Candida steatolytica, and Candida shehatae were subjected to fermentations in synthetic media containing mixtures of glucose and xylose. In all cases, repression of xylose uptake by glucose was observed, although the extent of repression was different with each yeast. While Candida shehatae was found to utilize xylose effectively in the presence of approximately 5% (w/v) glucose, Candida steatolytica could only utilize xylose when the glucose concentration was below 3% (w/v), and Pichia stipitis required the glucose concentration in the medium to b
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4

Zheng, Liyuan, Shan Wei, Meiling Wu, et al. "Improving Xylose Fermentation in Saccharomyces cerevisiae by Expressing Nuclear-Localized Hexokinase 2." Microorganisms 8, no. 6 (2020): 856. http://dx.doi.org/10.3390/microorganisms8060856.

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Understanding the relationship between xylose and the metabolic regulatory systems is a prerequisite to enhance xylose utilization in recombinant S. cerevisiae strains. Hexokinase 2 (Hxk2p) is an intracellular glucose sensor that localizes to the cytoplasm or the nucleus depending on the carbon source. Hxk2p interacts with Mig1p to regulate gene transcription in the nucleus. Here, we investigated the effect of nucleus-localized Hxk2p and Mig1p on xylose fermentation. The results show that the expression of HXK2S14A, which encodes a constitutively nucleus-localized Hxk2p, increased the xylose c
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5

Sonderegger, Marco, and Uwe Sauer. "Evolutionary Engineering of Saccharomyces cerevisiae for Anaerobic Growth on Xylose." Applied and Environmental Microbiology 69, no. 4 (2003): 1990–98. http://dx.doi.org/10.1128/aem.69.4.1990-1998.2003.

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ABSTRACT Xylose utilization is of commercial interest for efficient conversion of abundant plant material to ethanol. Perhaps the most important ethanol-producing organism, Saccharomyces cerevisiae, however, is incapable of xylose utilization. While S. cerevisiae strains have been metabolically engineered to utilize xylose, none of the recombinant strains or any other naturally occurring yeast has been able to grow anaerobically on xylose. Starting with the recombinant S. cerevisiae strain TMB3001 that overexpresses the xylose utilization pathway from Pichia stipitis, in this study we develope
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Klimacek, Mario, Stefan Krahulec, Uwe Sauer, and Bernd Nidetzky. "Limitations in Xylose-Fermenting Saccharomyces cerevisiae, Made Evident through Comprehensive Metabolite Profiling and Thermodynamic Analysis." Applied and Environmental Microbiology 76, no. 22 (2010): 7566–74. http://dx.doi.org/10.1128/aem.01787-10.

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ABSTRACT Little is known about how the general lack of efficiency with which recombinant Saccharomyces cerevisiae strains utilize xylose affects the yeast metabolome. Quantitative metabolomics was therefore performed for two xylose-fermenting S. cerevisiae strains, BP000 and BP10001, both engineered to produce xylose reductase (XR), NAD+-dependent xylitol dehydrogenase and xylulose kinase, and the corresponding wild-type strain CEN.PK 113-7D, which is not able to metabolize xylose. Contrary to BP000 expressing an NADPH-preferring XR, BP10001 expresses an NADH-preferring XR. An updated protocol
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7

Ikawa, Yumi, Sayaka Ohnishi, Akiko Shoji, Ayako Furutani, and Seiji Tsuge. "Concomitant Regulation by a LacI-Type Transcriptional Repressor XylR on Genes Involved in Xylan and Xylose Metabolism and the Type III Secretion System in Rice Pathogen Xanthomonas oryzae pv. oryzae." Molecular Plant-Microbe Interactions® 31, no. 6 (2018): 605–13. http://dx.doi.org/10.1094/mpmi-11-17-0277-r.

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The hypersensitive response and pathogenicity (hrp) genes of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, encode components of the type III secretion system and are essential for virulence. Expression of hrp genes is regulated by two key hrp regulators, HrpG and HrpX; HrpG regulates hrpX and hrpA, and HrpX regulates the other hrp genes on hrpB-hrpF operons. We previously reported the sugar-dependent quantitative regulation of HrpX; the regulator highly accumulates in the presence of xylose, followed by high hrp gene expression. Here, we found that, in a mut
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8

Liu, Yunhao, Paul B. Rainey, and Xue-Xian Zhang. "Molecular mechanisms of xylose utilization byPseudomonas fluorescens: overlapping genetic responses to xylose, xylulose, ribose and mannitol." Molecular Microbiology 98, no. 3 (2015): 553–70. http://dx.doi.org/10.1111/mmi.13142.

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9

Meijnen, Jean-Paul, Johannes H. de Winde, and Harald J. Ruijssenaars. "Engineering Pseudomonas putida S12 for Efficient Utilization of d-Xylose and l-Arabinose." Applied and Environmental Microbiology 74, no. 16 (2008): 5031–37. http://dx.doi.org/10.1128/aem.00924-08.

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ABSTRACT The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose as a substrate by expressing xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli. The initial yield on xylose was low (9% [g CDW g substrate−1], where CDW is cell dry weight), and the growth rate was poor (0.01 h−1). The main cause of the low yield was the oxidation of xylose into the dead-end product xylonate by endogenous glucose dehydrogenase (Gcd). Subjecting the XylAB-expressing P. putida S12 to laboratory evolution yielded a strain that efficiently utilized xylose (yield, 52%
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10

Erbeznik, Milutin, Karl A. Dawson, and Herbert J. Strobel. "Cloning and Characterization of Transcription of the xylAB Operon in Thermoanaerobacter ethanolicus." Journal of Bacteriology 180, no. 5 (1998): 1103–9. http://dx.doi.org/10.1128/jb.180.5.1103-1109.1998.

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ABSTRACT The genes encoding xylose isomerase (xylA) and xylulose kinase (xylB) from the thermophilic anaerobeThermoanaerobacter ethanolicus were found to constitute an operon with the transcription initiation site 169 nucleotides upstream from the previously assigned (K. Dekker, H. Yamagata, K. Sakaguchi, and S. Udaka, Agric. Biol. Chem. 55:221–227, 1991) promoter region. The bicistronic xylAB mRNA was processed by cleavage within the 5′-terminal portion of the XylB-coding sequence. Transcription ofxylAB was induced in the presence of xylose, and, unlike in all other xylose-utilizing bacteria
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11

Park, Jongbeom, Sujeong Park, Grace Evelina, et al. "Metabolic Engineering of Komagataella phaffii for Xylose Utilization from Cellulosic Biomass." Molecules 29, no. 23 (2024): 5695. https://doi.org/10.3390/molecules29235695.

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Cellulosic biomass hydrolysates are rich in glucose and xylose, but most microorganisms, including Komagataella phaffii, are unable to utilize xylose effectively. To address this limitation, we engineered a K. phaffii strain optimized for xylose metabolism through the xylose oxidoreductase pathway and promoter optimization. A promoter library with varying strengths was used to fine-tune the expression levels of the XYL1, XYL2, and XYL3 genes, resulting in a strain with a strong promoter for XYL2 and weaker promoters for XYL1 and XYL3. This engineered strain exhibited superior growth, achieving
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12

Koirala, Santosh, Xiaoyi Wang, and Christopher V. Rao. "Reciprocal Regulation of l-Arabinose and d-Xylose Metabolism in Escherichia coli." Journal of Bacteriology 198, no. 3 (2015): 386–93. http://dx.doi.org/10.1128/jb.00709-15.

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ABSTRACTGlucose is known to inhibit the transport and metabolism of many sugars inEscherichia coli. This mechanism leads to its preferential consumption. Far less is known about the preferential utilization of nonglucose sugars inE. coli. Two exceptions arel-arabinose andd-xylose. Previous studies have shown thatl-arabinose inhibitsd-xylose metabolism inEscherichia coli. This repression results froml-arabinose-bound AraC binding to the promoter of thed-xylose metabolic genes and inhibiting their expression. This mechanism, however, has not been explored in single cells. Both thel-arabinose and
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13

Van Zyl, C., B. A. Prior, S. G. Kilian, and J. L. F. Kock. "D-Xylose Utilization by Saccharomyces cerevisiae." Microbiology 135, no. 11 (1989): 2791–98. http://dx.doi.org/10.1099/00221287-135-11-2791.

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14

Song, S. "Utilization of ?-ribose through ?-xylose transporter." FEMS Microbiology Letters 163, no. 2 (1998): 255–61. http://dx.doi.org/10.1016/s0378-1097(98)00180-3.

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15

Wu, Meiling, Hongxing Li, Shan Wei, et al. "Simulating Extracellular Glucose Signals Enhances Xylose Metabolism in Recombinant Saccharomyces cerevisiae." Microorganisms 8, no. 1 (2020): 100. http://dx.doi.org/10.3390/microorganisms8010100.

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Efficient utilization of both glucose and xylose from lignocellulosic biomass would be economically beneficial for biofuel production. Recombinant Saccharomyces cerevisiae strains with essential genes and metabolic networks for xylose metabolism can ferment xylose; however, the efficiency of xylose fermentation is much lower than that of glucose, the preferred carbon source of yeast. Implications from our previous work suggest that activation of the glucose sensing system may benefit xylose metabolism. Here, we show that deleting cAMP phosphodiesterase genes PDE1 and PDE2 increased PKA activit
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16

Sizemore, C., B. Wieland, F. Götz, and W. Hillen. "Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level." Journal of Bacteriology 174, no. 9 (1992): 3042–48. http://dx.doi.org/10.1128/jb.174.9.3042-3048.1992.

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17

Xiong, Xiaochao, Xi Wang, and Shulin Chen. "Engineering of a Xylose Metabolic Pathway in Rhodococcus Strains." Applied and Environmental Microbiology 78, no. 16 (2012): 5483–91. http://dx.doi.org/10.1128/aem.08022-11.

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ABSTRACTThe two metabolically versatile actinobacteriaRhodococcus opacusPD630 andR. jostiiRHA1 can efficiently convert diverse organic substrates into neutral lipids mainly consisting of triacylglycerol (TAG), the precursor of energy-rich hydrocarbon. Neither, however, is able to utilize xylose, the important component present in lignocellulosic biomass, as the carbon source for growth and lipid accumulation. In order to broaden their substrate utilization range, the metabolic pathway ofd-xylose utilization was introduced into these two strains. This was accomplished by heterogenous expression
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18

Wang, Meng, Chenzhao Yu, and Huimin Zhao. "Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization." Biotechnology and Bioengineering 113, no. 3 (2015): 484–91. http://dx.doi.org/10.1002/bit.25724.

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19

Xin, Fengxue, Yi-Rui Wu, and Jianzhong He. "Simultaneous Fermentation of Glucose and Xylose to Butanol by Clostridium sp. Strain BOH3." Applied and Environmental Microbiology 80, no. 15 (2014): 4771–78. http://dx.doi.org/10.1128/aem.00337-14.

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ABSTRACTCellulose and hemicellulose constitute the major components in sustainable feedstocks which could be used as substrates for biofuel generation. However, following hydrolysis to monomer sugars, the solventogenicClostridiumwill preferentially consume glucose due to transcriptional repression of xylose utilization genes. This is one of the major barriers in optimizing lignocellulosic hydrolysates that produce butanol. Unlike studies on existing bacteria, this study demonstrates that newly reportedClostridiumsp. strain BOH3 is capable of fermenting 60 g/liter of xylose to 14.9 g/liter buta
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20

Díaz-Fernández, David, Gloria Muñoz-Fernández, Victoria Isabel Martín, José Luis Revuelta, and Alberto Jiménez. "Sugar transport for enhanced xylose utilization in Ashbya gossypii." Journal of Industrial Microbiology & Biotechnology 47, no. 12 (2020): 1173–79. http://dx.doi.org/10.1007/s10295-020-02320-5.

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AbstractThe co-utilization of mixed (pentose/hexose) sugars constitutes a challenge for microbial fermentations. The fungus Ashbya gossypii, which is currently exploited for the industrial production of riboflavin, has been presented as an efficient biocatalyst for the production of biolipids using xylose-rich substrates. However, the utilization of xylose in A. gossypii is hindered by hexose sugars. Three A. gossypii homologs (AFL204C, AFL205C and AFL207C) of the yeast HXT genes that code for hexose transporters have been identified and characterized by gene-targeting approaches. Significant
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21

Rhee, Mun Su, Lusha Wei, Neha Sawhney, et al. "Engineering the Xylan Utilization System in Bacillus subtilis for Production of Acidic Xylooligosaccharides." Applied and Environmental Microbiology 80, no. 3 (2013): 917–27. http://dx.doi.org/10.1128/aem.03246-13.

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ABSTRACTXylans are the predominant polysaccharides in hemicelluloses and an important potential source of biofuels and chemicals. The ability ofBacillus subtilissubsp.subtilisstrain 168 to utilize xylans has been ascribed to secreted glycoside hydrolase family 11 (GH11) and GH30 endoxylanases, encoded by thexynAandxynCgenes, respectively. Both of these enzymes have been defined with respect to structure and function. In this study, the effects of deletion of thexynAandxynCgenes, individually and in combination, were evaluated for xylan utilization and formation of acidic xylooligosaccharides.
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22

Nobre, Alexandra, Cândida Lucas, and Cecília Leão. "Transport and Utilization of Hexoses and Pentoses in the Halotolerant Yeast Debaryomyces hansenii." Applied and Environmental Microbiology 65, no. 8 (1999): 3594–98. http://dx.doi.org/10.1128/aem.65.8.3594-3598.1999.

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ABSTRACT Debaryomyces hansenii is a yeast species that is known for its halotolerance. This organism has seldom been mentioned as a pentose consumer. In the present work, a strain of this species was investigated with respect to the utilization of pentoses and hexoses in mixtures and as single carbon sources. Growth parameters were calculated for batch aerobic cultures containing pentoses, hexoses, and mixtures of both types of sugars. Growth on pentoses was slower than growth on hexoses, but the values obtained for biomass yields were very similar with the two types of sugars. Furthermore, wh
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23

Blazeck, John, and Hal Alper. "Uncovering latent xylose utilization potential inSaccharomyces cerevisiae." Biofuels 1, no. 5 (2010): 681–84. http://dx.doi.org/10.4155/bfs.10.50.

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24

Li, Haibo, and Hal S. Alper. "Enabling xylose utilization inYarrowia lipolyticafor lipid production." Biotechnology Journal 11, no. 9 (2016): 1230–40. http://dx.doi.org/10.1002/biot.201600210.

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25

Kawaguchi, Hideo, Alain A. Vert�s, Shohei Okino, Masayuki Inui, and Hideaki Yukawa. "Engineering of a Xylose Metabolic Pathway in Corynebacterium glutamicum." Applied and Environmental Microbiology 72, no. 5 (2006): 3418–28. http://dx.doi.org/10.1128/aem.72.5.3418-3428.2006.

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ABSTRACT The aerobic microorganism Corynebacterium glutamicum was metabolically engineered to broaden its substrate utilization range to include the pentose sugar xylose, which is commonly found in agricultural residues and other lignocellulosic biomass. We demonstrated the functionality of the corynebacterial xylB gene encoding xylulokinase and constructed two recombinant C. glutamicum strains capable of utilizing xylose by cloning the Escherichia coli gene xylA encoding xylose isomerase, either alone (strain CRX1) or in combination with the E. coli gene xylB (strain CRX2). These genes were p
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26

Träff, K. L., R. R. Otero Cordero, W. H. van Zyl, and B. Hahn-Hägerdal. "Deletion of the GRE3 Aldose Reductase Gene and Its Influence on Xylose Metabolism in Recombinant Strains of Saccharomyces cerevisiae Expressing thexylA and XKS1 Genes." Applied and Environmental Microbiology 67, no. 12 (2001): 5668–74. http://dx.doi.org/10.1128/aem.67.12.5668-5674.2001.

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ABSTRACT Saccharomyces cerevisiae ferments hexoses efficiently but is unable to ferment xylose. When the bacterial enzyme xylose isomerase (XI) from Thermus thermophilus was produced in S. cerevisiae, xylose utilization and ethanol formation were demonstrated. In addition, xylitol and acetate were formed. An unspecific aldose reductase (AR) capable of reducing xylose to xylitol has been identified inS. cerevisiae. The GRE3gene, encoding the AR enzyme, was deleted in S.cerevisiae CEN.PK2-1C, yielding YUSM1009a. XI fromT. thermophilus was produced, and endogenous xylulokinase from S.cerevisiae w
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27

Wahlbom, C. Fredrik, Ricardo R. Cordero Otero, Willem H. van Zyl, Bärbel Hahn-Hägerdal, and Leif J. Jönsson. "Molecular Analysis of a Saccharomyces cerevisiae Mutant with Improved Ability To Utilize Xylose Shows Enhanced Expression of Proteins Involved in Transport, Initial Xylose Metabolism, and the Pentose Phosphate Pathway." Applied and Environmental Microbiology 69, no. 2 (2003): 740–46. http://dx.doi.org/10.1128/aem.69.2.740-746.2003.

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ABSTRACT Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samples for analysis were withdrawn from chemostat cultures. The characteristics of S. cerevisiae TMB 3399 and TMB 3400 grown on glucose and on a mixture of glucose and xylose, as well as of S. cerevisiae TMB 3400 grown on only xylose, were investigated. The strains were cult
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28

Wisselink, H. Wouter, Maurice J. Toirkens, Qixiang Wu, Jack T. Pronk, and Antonius J. A. van Maris. "Novel Evolutionary Engineering Approach for Accelerated Utilization of Glucose, Xylose, and Arabinose Mixtures by Engineered Saccharomyces cerevisiae Strains." Applied and Environmental Microbiology 75, no. 4 (2008): 907–14. http://dx.doi.org/10.1128/aem.02268-08.

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ABSTRACT Lignocellulosic feedstocks are thought to have great economic and environmental significance for future biotechnological production processes. For cost-effective and efficient industrial processes, complete and fast conversion of all sugars derived from these feedstocks is required. Hence, simultaneous or fast sequential fermentation of sugars would greatly contribute to the efficiency of production processes. One of the main challenges emerging from the use of lignocellulosics for the production of ethanol by the yeast Saccharomyces cerevisiae is efficient fermentation of d-xylose an
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29

Sirithep, Kanokwadee, Fei Xiao, Nachon Raethong, et al. "Probing Carbon Utilization of Cordyceps militaris by Sugar Transportome and Protein Structural Analysis." Cells 9, no. 2 (2020): 401. http://dx.doi.org/10.3390/cells9020401.

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Beyond comparative genomics, we identified 85 sugar transporter genes in Cordyceps militaris, clustering into nine subfamilies as sequence- and phylogenetic-based functional classification, presuming the versatile capability of the fungal growths on a range of sugars. Further analysis of the global gene expression patterns of C. militaris showed 123 genes were significantly expressed across the sucrose, glucose, and xylose cultures. The sugar transporters specific for pentose were then identified by gene-set enrichment analysis. Of them, the putative pentose transporter, CCM_06358 gene, was hi
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30

Kim, Byoungjin, Jing Du, Dawn T. Eriksen, and Huimin Zhao. "Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels." Applied and Environmental Microbiology 79, no. 3 (2012): 931–41. http://dx.doi.org/10.1128/aem.02736-12.

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ABSTRACTBalancing the flux of a heterologous metabolic pathway by tuning the expression and properties of the pathway enzymes is difficult, but it is critical to realizing the full potential of microbial biotechnology. One prominent example is the metabolic engineering of aSaccharomyces cerevisiaestrain harboring a heterologous xylose-utilizing pathway for cellulosic-biofuel production, which remains a challenge even after decades of research. Here, we developed a combinatorial pathway-engineering approach to rapidly create a highly efficient xylose-utilizing pathway for ethanol production by
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Bolotnikova, Olga, Julia Bazarnova, Ekaterina Aronova, Natalia Mikhailova, Tatiana Bolotnikova, and Jing Pu. "Spent sulphite liquor utilization by xylose-assimilating yeast pachysolen tannophilus, capable of bioethanol producing." E3S Web of Conferences 140 (2019): 02008. http://dx.doi.org/10.1051/e3sconf/201914002008.

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The xylose-assimilating capacity of yeast Pachysolen tannophilus to utilize sugars in spent sulphite liquor samples (pulp mill waste) with a different concentration of hexoses and pentoses was studied. The consumption of hexoses (D-glucose, D-mannose, D-galactose) and pentose (D-xylose) in such substrates reached 90.0-97.5% and 49.12-67.45%, respectively. The ethanol production from sugars in spent sulphite liquor by different strains of the yeast P. tannophilus was demonstrated. The maximum specific rate and ethanol yield reached 9.32-11.45 g l-1 and 0.28-0.37 g g sugars-1, respectively. Thus
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32

Runquist, David, B�rbel Hahn-H�gerdal, and Maurizio Bettiga. "Increased Ethanol Productivity in Xylose-Utilizing Saccharomyces cerevisiae via a Randomly Mutagenized Xylose Reductase." Applied and Environmental Microbiology 76, no. 23 (2010): 7796–802. http://dx.doi.org/10.1128/aem.01505-10.

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ABSTRACT Baker's yeast (Saccharomyces cerevisiae) has been genetically engineered to ferment the pentose sugar xylose present in lignocellulose biomass. One of the reactions controlling the rate of xylose utilization is catalyzed by xylose reductase (XR). In particular, the cofactor specificity of XR is not optimized with respect to the downstream pathway, and the reaction rate is insufficient for high xylose utilization in S. cerevisiae. The current study describes a novel approach to improve XR for ethanol production in S. cerevisiae. The cofactor binding region of XR was mutated by error-pr
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33

Luo, Kui, Xiaolong Guo, Huihui Zhang, Hongxin Fu, and Jufang Wang. "The Physiological Functions of AbrB on Sporulation, Biofilm Formation and Carbon Source Utilization in Clostridium tyrobutyricum." Bioengineering 9, no. 10 (2022): 575. http://dx.doi.org/10.3390/bioengineering9100575.

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As a pleiotropic regulator, Antibiotic resistant protein B (AbrB) was reported to play important roles in various cellular processes in Bacilli and some Clostridia strains. In Clostridium tyrobutyricum, abrB (CTK_C 00640) was identified to encode AbrB by amino acid sequence alignment and functional domain prediction. The results of abrB deletion or overexpression in C. tyrobutyricum showed that AbrB not only exhibited the reported characteristics such as the negative regulation on sporulation, positive effects on biofilm formation and stress resistance but also exhibited new functions, especia
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34

Meijnen, Jean-Paul, Johannes H. de Winde, and Harald J. Ruijssenaars. "Establishment of Oxidative d-Xylose Metabolism in Pseudomonas putida S12." Applied and Environmental Microbiology 75, no. 9 (2009): 2784–91. http://dx.doi.org/10.1128/aem.02713-08.

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ABSTRACT The oxidative d-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on d-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g d-xylose−1) and a maximum growth rate of 0.21 h−1. Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on d-xylose. Only the xylD gene, encoding d-xylonate dehydratase, proved to be essential for establishing an oxidative d-xylose cataboli
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35

Sizemore, Christine, Eberhard Buchner, Thomas Rygus, Claudia Witke, Friedrich Götz, and Wolfgang Hillen. "Organization, promoter analysis and transcriptional regulation of the Staphylococcus xylosus xylose utilization operon." Molecular and General Genetics MGG 227, no. 3 (1991): 377–84. http://dx.doi.org/10.1007/bf00273926.

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36

Chung, Nguyen Hoang, Nguyen Thi Thu Hoai, and Le Quang Dien. "Synthesis of furfural from Acacia mangium wood sawdust‐derived xylose by continuous distillation method using sulfonated carbonaceous catalyst from the same source." Vietnam Journal of Chemistry 58, no. 4 (2020): 494–99. http://dx.doi.org/10.1002/vjch.202000012.

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AbstractIn this study, the hydrolysate containing ~15 g/L of xylose, which was obtained from Acacia mangium wood sawdust was used for the synthesis of xylose by a continuous distillation method. The synthesis was conducted at 150 °C for 7 h using a carbon‐based sulfonated catalyst (CBSC) from the same biomass source with an acid density of about 4.0 mmol/g. The CBSC showed its efficient catalytic activity in the dehydration of xylose to furfural. Under the optimal catalyst dosage of 20 wt% over xylose, the yield of furfural was 20.4 mol% and 34 wt% of xylose was transformed to furfural. The re
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37

Bonthong, Pawarin, Benjarat Bunterngsook, Wuttichai Mhuantong, et al. "Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production." Journal of Fungi 11, no. 6 (2025): 453. https://doi.org/10.3390/jof11060453.

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The development of yeast cell factories for efficient xylose utilization and xylitol production is crucial for advancing sustainable biotechnological processes. Xylose, a major component of lignocellulosic biomass, presents challenges for microbial conversion due to its complex metabolic pathways. This study presents the genomic perspective and xylitol production capability of a novel xylose utilizing yeast Cyberlindnera fabianii TBRC 4498. Genome sequencing and functional annotation revealed key metabolic networks and genes involved in the xylose metabolism pathway, providing insights into th
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Liu, Tingting, Shuangcheng Huang, and Anli Geng. "Recombinant Diploid Saccharomyces cerevisiae Strain Development for Rapid Glucose and Xylose Co-Fermentation." Fermentation 4, no. 3 (2018): 59. http://dx.doi.org/10.3390/fermentation4030059.

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Cost-effective production of cellulosic ethanol requires robust microorganisms for rapid co-fermentation of glucose and xylose. This study aims to develop a recombinant diploid xylose-fermenting Saccharomyces cerevisiae strain for efficient conversion of lignocellulosic biomass sugars to ethanol. Episomal plasmids harboring codon-optimized Piromyces sp. E2 xylose isomerase (PirXylA) and Orpinomyces sp. ukk1 xylose (OrpXylA) genes were constructed and transformed into S. cerevisiae. The strain harboring plasmids with tandem PirXylA was favorable for xylose utilization when xylose was used as th
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Feng, Linjuan, Junhao Xu, Cuifang Ye, et al. "Metabolic Engineering of Pichia pastoris for the Production of Triacetic Acid Lactone." Journal of Fungi 9, no. 4 (2023): 494. http://dx.doi.org/10.3390/jof9040494.

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Triacetic acid lactone (TAL) is a promising renewable platform polyketide with broad biotechnological applications. In this study, we constructed an engineered Pichia pastoris strain for the production of TAL. We first introduced a heterologous TAL biosynthetic pathway by integrating the 2-pyrone synthase encoding gene from Gerbera hybrida (Gh2PS). We then removed the rate-limiting step of TAL synthesis by introducing the posttranslational regulation-free acetyl-CoA carboxylase mutant encoding gene from S. cerevisiae (ScACC1*) and increasing the copy number of Gh2PS. Finally, to enhance intrac
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Song, Sukgil, and Chankyu Park. "Utilization of d-ribose through d-xylose transporter." FEMS Microbiology Letters 163, no. 2 (1998): 255–61. http://dx.doi.org/10.1111/j.1574-6968.1998.tb13054.x.

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McMillan, James D., and Brian L. Boynton. "Arabinose utilization by xylose-fermenting yeasts and fungi." Applied Biochemistry and Biotechnology 45-46, no. 1 (1994): 569–84. http://dx.doi.org/10.1007/bf02941831.

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42

Stevis, Panayiotis E., and Nancy W. Y. Ho. "Positive selection vectors based on xylose utilization suppression." Gene 55, no. 1 (1987): 67–74. http://dx.doi.org/10.1016/0378-1119(87)90249-6.

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Banerjee, S., A. Archana, and T. Satyanarayana. "Xylanolytic activity and xylose utilization by thermophilic molds." Folia Microbiologica 40, no. 3 (1995): 279–82. http://dx.doi.org/10.1007/bf02814208.

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44

Devarapalli, Pratap, Nishad Deshpande, and Rajkumar R. Hirwani. "Xylose utilization in ethanol production: a patent landscape." Biofuels, Bioproducts and Biorefining 10, no. 5 (2016): 534–41. http://dx.doi.org/10.1002/bbb.1664.

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45

Jin, Yong-Su, Jose M. Laplaza, and Thomas W. Jeffries. "Saccharomyces cerevisiae Engineered for Xylose Metabolism Exhibits a Respiratory Response." Applied and Environmental Microbiology 70, no. 11 (2004): 6816–25. http://dx.doi.org/10.1128/aem.70.11.6816-6825.2004.

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ABSTRACT Native strains of Saccharomyces cerevisiae do not assimilate xylose. S. cerevisiae engineered for d-xylose utilization through the heterologous expression of genes for aldose reductase (XYL1), xylitol dehydrogenase (XYL2), and d-xylulokinase (XYL3 or XKS1) produce only limited amounts of ethanol in xylose medium. In recombinant S. cerevisiae expressing XYL1, XYL2, and XYL3, mRNA transcript levels for glycolytic, fermentative, and pentose phosphate enzymes did not change significantly on glucose or xylose under aeration or oxygen limitation. However, expression of genes encoding the tr
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46

Xiao, Han, Yang Gu, Yuanyuan Ning, et al. "Confirmation and Elimination of Xylose Metabolism Bottlenecks in Glucose Phosphoenolpyruvate-Dependent Phosphotransferase System-Deficient Clostridium acetobutylicum for Simultaneous Utilization of Glucose, Xylose, and Arabinose." Applied and Environmental Microbiology 77, no. 22 (2011): 7886–95. http://dx.doi.org/10.1128/aem.00644-11.

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ABSTRACTEfficient cofermentation ofd-glucose,d-xylose, andl-arabinose, three major sugars present in lignocellulose, is a fundamental requirement for cost-effective utilization of lignocellulosic biomass. The Gram-positive anaerobic bacteriumClostridium acetobutylicum, known for its excellent capability of producing ABE (acetone, butanol, and ethanol) solvent, is limited in using lignocellulose because of inefficient pentose consumption when fermenting sugar mixtures. To overcome this substrate utilization defect, a predictedglcGgene, encoding enzyme II of thed-glucose phosphoenolpyruvate-depe
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Zhang, Guo-Chang, Jing-Jing Liu, and Wen-Tao Ding. "Decreased Xylitol Formation during Xylose Fermentation in Saccharomyces cerevisiae Due to Overexpression of Water-Forming NADH Oxidase." Applied and Environmental Microbiology 78, no. 4 (2011): 1081–86. http://dx.doi.org/10.1128/aem.06635-11.

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ABSTRACTThe recombinant xylose-fermentingSaccharomyces cerevisiaestrain harboring xylose reductase (XR) and xylitol dehydrogenase (XDH) fromScheffersomyces stipitisrequires NADPH and NAD+, creates cofactor imbalance, and causes xylitol accumulation during growth ond-xylose. To solve this problem,noxE, encoding a water-forming NADH oxidase fromLactococcus lactisdriven by thePGK1promoter, was introduced into the xylose-utilizing yeast strain KAM-3X. A cofactor microcycle was set up between the utilization of NAD+by XDH and the formation of NAD+by water-forming NADH oxidase. Overexpression ofnoxE
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Long, Tanya M., Yi-Kai Su, Jennifer Headman, Alan Higbee, Laura B. Willis, and Thomas W. Jeffries. "Cofermentation of Glucose, Xylose, and Cellobiose by the Beetle-Associated Yeast Spathaspora passalidarum." Applied and Environmental Microbiology 78, no. 16 (2012): 5492–500. http://dx.doi.org/10.1128/aem.00374-12.

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ABSTRACTFermentation of cellulosic and hemicellulosic sugars from biomass could resolve food-versus-fuel conflicts inherent in the bioconversion of grains. However, the inability to coferment glucose and xylose is a major challenge to the economical use of lignocellulose as a feedstock. Simultaneous cofermentation of glucose, xylose, and cellobiose is problematic for most microbes because glucose represses utilization of the other saccharides. Surprisingly, the ascomycetous, beetle-associated yeastSpathaspora passalidarum, which ferments xylose and cellobiose natively, can also coferment these
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Bazarnova, Yuliya, Olga Bolotnikova, Natalia Michailova, and Jing Pu. "Optimization of parameters of alcohol fermentation of xylose-containing inedible substrates using the yeast Pachysolen Tannophilus." MATEC Web of Conferences 245 (2018): 18006. http://dx.doi.org/10.1051/matecconf/201824518006.

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This work presents economical and ecological advances of microbiological utilization of inedible sources of plant biomass, procedure which is associated with bioethanol obtaining. We study influence of forced aeration and initial concentration of biomass of xylose-assimilating yeast P. tannophilus Y-1532/B2 on ethanol output from various xylose-containing substrates during periodical fermentation. The highest ethanol output is observed for OTR values equal to 5.0-8.0 mMole/l×h and yeast seeding density equal to 0.25 g a.d.s./g of substrate sugars. We show the possibility for intensification of
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Gu, Pengfei, Fangfang Li, and Zhaosong Huang. "Engineering Escherichia coli for Isobutanol Production from Xylose or Glucose–Xylose Mixture." Microorganisms 11, no. 10 (2023): 2573. http://dx.doi.org/10.3390/microorganisms11102573.

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Aiming to overcome the depletion of fossil fuels and serious environmental pollution, biofuels such as isobutanol have garnered increased attention. Among different synthesis methods, the microbial fermentation of isobutanol from raw substrate is a promising strategy due to its low cost and environmentally friendly and optically pure products. As an important component of lignocellulosics and the second most common sugar in nature, xylose has become a promising renewable resource for microbial production. However, bottlenecks in xylose utilization limit its wide application as substrates. In t
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