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Journal articles on the topic 'Y. Lipa'

Author: Grafiati

Published: 28 May 2022

Last updated: 31 July 2025

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1

El-Aziz, Abeer M. Abd, Mohamed A. Shaker, and Mona I. Shaaban. "Enhanced Biocatalytic Activity of Recombinant Lipase Immobilized on Gold Nanoparticles." Current Pharmaceutical Biotechnology 20, no. 6 (2019): 497–505. http://dx.doi.org/10.2174/1389201020666190416144650.

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Background: Bacterial lipases especially Pseudomonas lipases are extensively used for different biotechnological applications. Objectives: With the better understanding and progressive needs for improving its activity in accordance with the growing market demand, we aimed in this study to improve the recombinant production and biocatalytic activity of lipases via surface conjugation on gold nanoparticles. Methods: The full length coding sequences of lipase gene (lipA), lipase specific foldase gene (lipf) and dual cassette (lipAf) gene were amplified from the genomic DNA of Pseudomonas aerugino

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Zhang, Hanrui, Jianting Shi, Melanie A. Hachet, et al. "CRISPR/Cas9-Mediated Gene Editing in Human iPSC-Derived Macrophage Reveals Lysosomal Acid Lipase Function in Human Macrophages—Brief Report." Arteriosclerosis, Thrombosis, and Vascular Biology 37, no. 11 (2017): 2156–60. http://dx.doi.org/10.1161/atvbaha.117.310023.

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Objective— To gain mechanistic insights into the role of LIPA (lipase A), the gene encoding LAL (lysosomal acid lipase) protein, in human macrophages. Approach and Results— We used CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR–associated protein 9) technology to knock out LIPA in human induced pluripotent stem cells and then differentiate to macrophage (human-induced pluripotent stem cells–derived macrophage [IPSDM]) to explore the human macrophage LIPA loss-of-function phenotypes. LIPA was abundantly expressed in monocyte-derived macrophages and was markedly

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Westers, Helga, Peter G. Braun, Lidia Westers, et al. "Genes Involved in SkfA Killing Factor Production Protect a Bacillus subtilis Lipase against Proteolysis." Applied and Environmental Microbiology 71, no. 4 (2005): 1899–908. http://dx.doi.org/10.1128/aem.71.4.1899-1908.2005.

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ABSTRACT Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B. subtilis genome results in reduced amounts of extracellular LipA. Here we demonstrate that the reduced LipA levels are due to the absence of four genes, skfABCD, located in the prophage 1 region. Intact skfABCD genes are required not only for LipA production at wild-type levels by B. subtilis 168 but also under conditions of LipA overproduction. Notably, SkfA has bactericidal activity and, prob

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Evans, Trent D., Xiangyu Zhang, Reece E. Clark, et al. "Functional Characterization of LIPA (Lysosomal Acid Lipase) Variants Associated With Coronary Artery Disease." Arteriosclerosis, Thrombosis, and Vascular Biology 39, no. 12 (2019): 2480–91. http://dx.doi.org/10.1161/atvbaha.119.313443.

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Objective: LIPA (lysosomal acid lipase) mediates cholesteryl ester hydrolysis, and patients with rare loss-of-function mutations develop hypercholesterolemia and severe disease. Genome-wide association studies of coronary artery disease have identified several tightly linked, common intronic risk variants in LIPA which unexpectedly associate with increased mRNA expression. However, an exonic variant (rs1051338 resulting in T16P) in linkage with intronic variants lies in the signal peptide region and putatively disrupts trafficking. We sought to functionally investigate the net impact of this l

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Batacan, Romeo B., Mitch J. Duncan, Vincent J. Dalbo, Patrick S. Tucker, and Andrew S. Fenning. "Effects of Light Intensity Activity on CVD Risk Factors: A Systematic Review of Intervention Studies." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/596367.

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The effects of light intensity physical activity (LIPA) on cardiovascular disease (CVD) risk factors remain to be established. This review summarizes the effects of LIPA on CVD risk factors and CVD-related markers in adults. A systematic search of four electronic databases (PubMed, Academic Search Complete, SPORTDiscus, and CINAHL) examining LIPA and CVD risk factors (body composition, blood pressure, glucose, insulin, glycosylated hemoglobin, and lipid profile) and CVD-related markers (maximal oxygen uptake, heart rate, C-reactive protein, interleukin-6, tumor necrosis factor-alpha, and tumor

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Zimdars-Swartz, Sandra L. "Lipa Comes to Necedah." Nova Religio 21, no. 2 (2017): 100–110. http://dx.doi.org/10.1525/nr.2017.21.2.100.

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In the late summer and fall of 1950, Juliet Hughes, a self-appointed promoter of a Marian apparition at Lipa in the Philippines, joined the crowds assembling for apparitions of the Virgin in Necedah, Wisconsin. The story of Hughes’ visits to Necedah—including a miraculous rose petal she brought from Lipa as well as her meetings with visionary Mary Ann Van Hoof and a number of Necedah pilgrims—highlights the importance of person-to-person encounters at an active apparition site. Indeed, the events described here suggest that when miraculous objects and miracle stories are shared among various s

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Pulido, Ingrid Yamile, Erlide Prieto, Gilles Paul Pieffet, Lina Méndez, and Carlos A. Jiménez-Junca. "Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced." International Journal of Molecular Sciences 21, no. 11 (2020): 3925. http://dx.doi.org/10.3390/ijms21113925.

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This study aimed to express heterologously the lipase LipA from Pseudomonas aeruginosa PSA01 obtained from palm fruit residues. In previous approaches, LipA was expressed in Escherichia coli fused with its signal peptide and without its disulfide bond, displaying low activity. We cloned the mature LipA with its truncated chaperone Lif in a dual plasmid and overexpressed the enzyme in two E. coli strains: the traditional BL21 (DE3) and the SHuffle® strain, engineered to produce stable cytoplasmic disulfide bonds. We evaluated the effect of the disulfide bond on LipA stability using molecular dy

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Abriani, Andi. "Innovation of Tourism Products in Increasing Sales of Lipa Sabbe Sengkang to Entrepreneurs in Wajo Regency." Journal La Sociale 4, no. 2 (2023): 67–75. http://dx.doi.org/10.37899/journal-la-sociale.v4i2.816.

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This study aims to determine the Increase in Sales Volume of Lipa Sabbe Sengkang Tourism Products and the Increase in Volume of Lipa Sabbe Sengkang Tourism Products in Wajo Regency. The type of research uses qualitative methods, namely the presentation of data in the form of statements of words or descriptions of something in the form of sentences set forth in writing. The data collection techniques use observation, interviews, and documentation. The results of this study showed that tourism product innovation in increasing sales volume of Lipa Sabbe Sengkang to entrepreneurs in Wajo district.

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Cai, Pengfei, Wenyi Zhang, Sufei Jiang, et al. "Role of Mn-LIPA in Sex Hormone Regulation and Gonadal Development in the Oriental River Prawn, Macrobrachium nipponense." International Journal of Molecular Sciences 25, no. 3 (2024): 1399. http://dx.doi.org/10.3390/ijms25031399.

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This study investigates the role of lysosomal acid lipase (LIPA) in sex hormone regulation and gonadal development in Macrobrachium nipponense. The full-length Mn-LIPA cDNA was cloned, and its expression patterns were analyzed using quantitative real-time PCR (qPCR) in various tissues and developmental stages. Higher expression levels were observed in the hepatopancreas, cerebral ganglion, and testes, indicating the potential involvement of Mn-LIPA in sex differentiation and gonadal development. In situ hybridization experiments revealed strong Mn-LIPA signaling in the spermatheca and hepatopa

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Cahova, Monika, Helena Dankova, Eliska Palenickova, et al. "The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance." Biochemistry Research International 2012 (2012): 1–11. http://dx.doi.org/10.1155/2012/135723.

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We tested the hypothesis that TAG accumulation in the liver induced by short-term high-fat diet (HFD) in rats leads to the dysregulation of endogenous TAG degradation by lysosomal lipase (LIPA) via lysosomal pathway and is causally linked with the onset of hepatic insulin resistance. We found that LIPA could be translocated between qualitatively different depots (light and dense lysosomes). In contrast to dense lysosomal fraction, LIPA associated with light lysosomes exhibits high activity on both intracellular TAG and exogenous substrate and prandial- or diet-dependent regulation. On standard

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Busrah, Busrah. "PERANAN KOPERASI PRODUSEN LIPA’ SA’BE MANDAR DALAM MENINGKATKAN PENDAPATAN EKONOMI MASYARAKAT PENENUN SUTERA (STUDI KASUS DI BERU-BERU DESA ONGKO KEC. CAMPALGIAN)." J-Alif : Jurnal Penelitian Hukum Ekonomi Syariah dan Budaya Islam 3, no. 1 (2018): 13. https://doi.org/10.35329/jalif.v3i1.521.

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Penelitian ini membahas tentang peranan koperasi produsen Lipa’ Sa’be Mandar dalam meningkatkan pendapatan ekonomi masyarakat. Maka yang menjadi pokok permasalahan adalah bagaimana peningkatan penjualan koperasi produsen “Mandar Sutera” dari tahun ke tahun, selanjutnya bagaimana peranan koperasi produsen Lipa’ Sa’be Mandar dalam pengembangan eokonomi sosial masyarakat penenun kain Sutera di Desa Ongko, serta faktor-faktor apakah yang mempengaruhi koperasi produsen Lipa’ Sa’be Mandar. Dalam pengumpulan data di gunakan metode penelitian lapangan seperti Obserpasi, Wawancara, Dokumentasi dan stud

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Tzeng, Yih-Ling, Anup K. Datta, Cristy A. Strole, Michael A. Lobritz, Russell W. Carlson, and David S. Stephens. "Translocation and Surface Expression of Lipidated Serogroup B Capsular Polysaccharide in Neisseria meningitidis." Infection and Immunity 73, no. 3 (2005): 1491–505. http://dx.doi.org/10.1128/iai.73.3.1491-1505.2005.

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ABSTRACT The capsule of N. meningitidis serogroup B, (α2→8)-linked polysialic acid and the capsules of other meningococcal serogroups and of other gram-negative bacterial pathogens are anchored in the outer membrane through a 1,2-diacylglycerol moiety. Previous work on the meningococcal cps complex in Escherichia coli K-12 indicated that deletion of genes designated lipA and lipB caused intracellular accumulation of hyperelongated capsule polymers lacking the phospholipid substitution. To better understand the role of lip and lipB in capsule expression in a meningococcal background, the locati

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-, Supratiwi Amir, and Faisal Syamsuddin -. "EKSISTENSI KAIN TENUN LIPA’SABBE DALAM MASYARAKAT SUKU BUGIS DI KOTA SENGKANG KABUPATEN WAJO SULAWESI SELATAN." SULUH: Jurnal Seni Desain Budaya 5, no. 2 (2022): 35–47. http://dx.doi.org/10.34001/jsuluh.v5i2.4232.

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Tradisi menenun dalam masyarakat suku Bugis merupakan keterampilan lokal genius. Tradisi ini menghasilkan sebuah kain tenun yang disebut lipa’ sabbe yang berarti sarung sutra. Eksistensi kain tenun lipa’ sabbe dalam masyakat suku Bugis di Kota Sengkang Kabupaten Wajo Sulawesi Selatan telah ditinjau melalui asal-usul kebudayaan menenun serta fungsi kain tenun lipa’ sabbe. Asal usul tradisi menenun dalam masyarakat suku Bugis menjelaskan teknologi alat tenun, bahan dasar dan teknik sederhana pembuatan lipa’ sabbe. Penelitian ini menggunakan metode analisis deskriptif kualitatif. Berdasarkan hasi

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Martin, Cara, David Roberts, Marjo van der Weide, et al. "Development of a PCR-Based Line Probe Assay for Identification of Fungal Pathogens." Journal of Clinical Microbiology 38, no. 10 (2000): 3735–42. http://dx.doi.org/10.1128/jcm.38.10.3735-3742.2000.

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We report on a reverse-hybridization line probe assay (LiPA) which when combined with PCR amplification detects and identifies clinically significant fungal pathogens including Candida,Aspergillus, and Cryptococcus species. DNA probes have been designed from the internal transcribed-spacer (ITS) regions of Candida albicans, Candida parapsilosis, Candida glabrata, Candida tropicalis, Candida krusei, Candida dubliniensis, Cryptococcus neoformans,Aspergillus fumigatus, Aspergillus versicolor,Aspergillus nidulans and Aspergillus flavus. The probes were incorporated into a LiPA for detection of bio

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Kok, R. G., C. B. Nudel, R. H. Gonzalez, I. M. Nugteren-Roodzant, and K. J. Hellingwerf. "Physiological factors affecting production of extracellular lipase (LipA) in Acinetobacter calcoaceticus BD413: fatty acid repression of lipA expression and degradation of LipA." Journal of bacteriology 178, no. 20 (1996): 6025–35. http://dx.doi.org/10.1128/jb.178.20.6025-6035.1996.

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Miller, Nancimae, Susanna Infante, and Tim Cleary. "Evaluation of the LiPA MYCOBACTERIA Assay for Identification of Mycobacterial Species from BACTEC 12B Bottles." Journal of Clinical Microbiology 38, no. 5 (2000): 1915–19. http://dx.doi.org/10.1128/jcm.38.5.1915-1919.2000.

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The LiPA MYCOBACTERIA (Innogenetics NV, Ghent, Belgium) assay was used to identify mycobacterial isolates using culture fluid from positive BACTEC 12B bottles. The LiPA method involves reverse hybridization of a biotinylated mycobacterial PCR fragment, a 16 to 23S rRNA spacer region, to oligonucleotide probes arranged in lines on a membrane strip, with detection via biotin-streptavidin coupling by a colorimetric system. This system identifies Mycobacteriumspecies and differentiates M. tuberculosis complex,M. avium-M. intracellulare complex, and the following mycobacterial species: M. avium, M.

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Moura, Bruno Pereira de, Bruna Priscila Colombo, Valter Paulo Neves Miranda, Isabella Toledo Caetano, Paulo Roberto dos Santos Amorim, and Jeffer Eidi Sasaki. "Sociodemographic determinants of sedentary behavior and physical activity assessed by triaxial accelerometry in Brazilian adults diagnosed with type 2 Diabetes." Cuadernos de Educación y Desarrollo 16, no. 7 (2024): e4799. http://dx.doi.org/10.55905/cuadv16n7-065.

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Sedentary behavior (SB) and physical activity (PA) are distinct physical behaviors influencing health outcomes, particularly in individuals with type 2 diabetes (T2D). Increased SB is associated with higher cardiometabolic risk, while PA, especially moderate-to-vigorous intensity physical activity (MVPA), is linked to reduced mortality and improved health. Given the diverse sociodemographic context of Brazil, understanding the determinants of SB and PA in Brazilian adults with T2D is essential. To investigate the association of sociodemographic and clinical factors with SB, light-intensity phy

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Mese, Sevim, Muzaffer Arikan, Aris Cakiris, et al. "Role of the line probe assay INNO-LiPA HBV DR and ultradeep pyrosequencing in detecting resistance mutations to nucleoside/nucleotide analogues in viral samples isolated from chronic hepatitis B patients." Journal of General Virology 94, no. 12 (2013): 2729–38. http://dx.doi.org/10.1099/vir.0.053041-0.

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Despite the effectiveness of nucleoside/nucleotide analogues in the treatment of chronic hepatitis B (CHB), their long-term administration is associated with the emergence of resistant hepatitis B virus (HBV) mutants. In this study, mutations resulting in antiviral resistance in HBV DNA samples isolated from 23 CHB patients (nine treatment naïve and 14 treated previously) were studied using a line probe assay (INNO-LiPA HBV DR; Innogenetics) and ultradeep pyrosequencing (UDPS) methods. Whilst the INNO-LiPA HBV DR showed no resistance mutations in HBV DNA samples from treatment-naive patients,

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Ochtavia, Gustia, Muhammad Sarjan, and Akhmad Qashlim. "Image Processing Application for Lipa Saqbe Mandar." Journal of Intelligent Computing and Health Informatics 2, no. 2 (2022): 43. http://dx.doi.org/10.26714/jichi.v2i2.8068.

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Seeing from today's technological developments, it is growing rapidly, but there are still many people, especially the younger generation today, whose understanding is still very lacking, in recognizing any motifs or patterns in Lipa Saqbe Mandar. From these problems, an image processing application is needed to recognize the Lipa Saqbe pattern that can be used to help facilitate the community or the younger generation, where the camera application will be directed to Lipa Saqbe and will then provide information or identify the name of the Lipa Saqbe pattern or motif. This system uses the temp

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Samad, Irfawandi, Herlina Ahmad, and F. Febryanti. "Peningkatan Kemampuan Representasi Matematis melalui Media Lipa’ Sa’be Mandar." Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam 9, no. 1 (2020): 57. http://dx.doi.org/10.35580/sainsmat91141912020.

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Penelitian ini bertujuan untuk meningkatkan kemampuan representasi matematis siswa di SMK Mega Link Majenemelalui media pembelajaran lipa’ sa’be Mandar.Jenis penelitian ini merupakan penelitian tindakan kelas yang dilaksanakan selama 2 siklus yang meliputi empat tahapan yaitu perencanaan, pelaksanaan observasi dan evaluasi serta refleksi.Teknik analisis menggunakan analisis statistika deskriptif. Hasil penelitian diperoleh nilai rata-rata kemampuan representasi matematis sebesar 82,65 dengan standar nilai KKM sebesar 70. Sedangkan nilai ketuntasan secara klasikal sebesar 84,21% dengan standar

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Xu, Lan, Elizaveta Padalko, Anja Oštrbenk, Mario Poljak, and Marc Arbyn. "Clinical Evaluation of INNO-LiPA HPV Genotyping EXTRA II Assay Using the VALGENT Framework." International Journal of Molecular Sciences 19, no. 9 (2018): 2704. http://dx.doi.org/10.3390/ijms19092704.

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In this diagnostic test validation study, we assessed the clinical accuracy and HPV genotyping performance of the INNO-LiPA HPV Genotyping Extra II (INNO-LiPA) within the VALGENT-3 framework. VALGENT is designed to assess the analytical and clinical performance of HPV tests with genotyping capacity. The VALGENT-3 panel comprised 1300 consecutive cervical cell specimens enriched with 300 samples with abnormal cytology obtained from women attending the Slovenian cervical cancer screening programme. The INNO-LiPA allows type-specific detection of 32 HPV types; however, for the clinical accuracy a

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Qutub, Mohammed O., Jeffrey J. Germer, Sjoerd P. H. Rebers, Jayawant N. Mandrekar, Marcel G. H. M. Beld, and Joseph D. C. Yao. "Simplified PCR protocols for INNO-LiPA HBV Genotyping and INNO-LiPA HBV PreCore assays." Journal of Clinical Virology 37, no. 3 (2006): 218–21. http://dx.doi.org/10.1016/j.jcv.2006.08.006.

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Kim, Sun-Hee, In-Hye Park, Sang-Cheol Lee, et al. "Discovery of three novel lipase (lipA 1, lipA 2, and lipA 3) and lipase-specific chaperone (lipB) genes present in Acinetobacter sp. DYL129." Applied Microbiology and Biotechnology 77, no. 5 (2008): 1041–51. http://dx.doi.org/10.1007/s00253-007-1242-z.

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Descamps, Diane, Vincent Calvez, Gilles Collin, et al. "Line Probe Assay for Detection of Human Immunodeficiency Virus Type 1 Mutations Conferring Resistance to Nucleoside Inhibitors of Reverse Transcriptase: Comparison with Sequence Analysis." Journal of Clinical Microbiology 36, no. 7 (1998): 2143–45. http://dx.doi.org/10.1128/jcm.36.7.2143-2145.1998.

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We compared the line probe assay (LiPA) to sequence analysis for the detection of mutations conferring resistance to nucleoside inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). Plasma samples from 40 patients who had received zidovudine, dideoxyinosine, and dideoxycytosine, alone or in combination, and who were enrolled in the ALTIS 2 clinical trial (lamivudine [3TC] plus stavudine) were tested at enrollment and at week 24. RT PCR products from plasma were used for LiPA, and DNA was used for sequence analysis. LiPA gave uninterpretable results for 8.5% of t

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De Vreese, Karen, Rachel Barylski, Fiona Pughe, et al. "Performance Characteristics of Updated INNO-LiPA Assays for Molecular Typing of Human Leukocyte Antigen A (HLA-A), HLA-B, and HLA-DQB1 Alleles." Clinical Diagnostic Laboratory Immunology 11, no. 2 (2004): 430–32. http://dx.doi.org/10.1128/cdli.11.2.430-432.2004.

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ABSTRACT We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.

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Kiky Nakesya A.S, Rusnah, Adi Sulaiman M.Siddiq, Andi Dian Angriani, and Alimuddin Tampa. "Eksplorasi Etnomatematika yang Terdapat dalam Corak Lipa’ Sa’be Mandar sebagai Sumber Belajar." Alauddin Journal of Mathematics Education 6, no. 1 (2024): 76–87. http://dx.doi.org/10.24252/ajme.v6i1.47034.

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Penelitian ini bertujuan untuk mengeksplorasi keterkaitan antara matematika dengan budaya yang terdapat pada corak Lipa’ Sa’be Mandar sebagai sumber belajar. Jenis penilitan yang digunakan yakni penelitian kualitatif dengan pendekatan etnografi. Subjek penelitian ini adalah warga Mandar bertempat di Desa Suruang Kecamatan Campalagian Kabupaten Polewali Mandar. Adapun instrumen yang digunakan yakni peneliti sendiri dan juga instrumen pendukung seperti panduan wawancara, akan tetapi wawancara berkembang menyesuaikan kebutuhan peneliti. Teknik pengumpulan data terdiri dari observasi, wawancara da

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Gonze, Bárbara, Thatiane Lopes Valentim Di Paschoale Ostolin, Evandro Sperandio, Rodolfo Arantes, Marcello Romiti, and Victor Dourado. "Effects of Substituting Sedentary Behavior with Light-Intensity or Moderate-to-Vigorous Physical Activity on Obesity Indices in Adults: A Prospective Short-Term Follow-Up Study." International Journal of Environmental Research and Public Health 18, no. 24 (2021): 13335. http://dx.doi.org/10.3390/ijerph182413335.

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Background: Sedentary behavior (SB) is an independent risk factor for cardiovascular diseases. We hypothesized that there may be benefits of replacing SB with light-intensity (LIPA) and moderate-to-vigorous (MVPA) physical activity. Substituting SB with LIPA and MVPA might be associated with body composition changes. Methods: We assessed body weight, body mass index (BMI), fat body mass (FBM), and physical activity level, as well as one-year changes, in 780 adults (EPIMOV Study). Results: We analyzed into 10-min blocks SB, LIPA, MVPA, and total wear time. After 14 ± 3 months of follow-up, ther

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Contardo Ayala, Ana, Jo Salmon, David Dunstan, Lauren Arundell, Kate Parker, and Anna Timperio. "Longitudinal Changes in Sitting Patterns, Physical Activity, and Health Outcomes in Adolescents." Children 6, no. 1 (2018): 2. http://dx.doi.org/10.3390/children6010002.

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This study examined two-year changes in patterns of activity and associations with body mass index (BMI) and waist circumference (WC) among adolescents. Inclinometers (activPAL) assessed sitting, sitting bouts, standing, stepping, and breaks from sitting. ActiGraph-accelerometers assessed sedentary time (SED), light-intensity physical activity (LIPA, stratified as low- and high-LIPA), and moderate-to-vigorous physical activity (MVPA). Anthropometric measures were objectively assessed at baseline and self-reported at follow-up. Data from 324 and 67 participants were obtained at baseline and fol

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Ahmad, Suhail, and Eiman Mokaddas. "Diversity of Nontuberculous Mycobacteria in Kuwait: Rapid Identification and Differentiation of Mycobacterium Species by Multiplex PCR, INNO-LiPA Mycobacteria v2 Assay and PCR Sequencing of rDNA." Medical Principles and Practice 28, no. 3 (2019): 208–15. http://dx.doi.org/10.1159/000498910.

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Objective: Nontuberculous mycobacteria (NTM) often cause disease that is clinically indistinguishable from tuberculosis. Specific identification is important as treatment varies according to Mycobacterium species causing the infection. This study used multiplex PCR (mPCR) assay for rapid differentiation of mycobacterial growth indicator tube 960 system (MGIT) cultures as Mycobacterium tuberculosis (MTB) or NTM together with INNO LiPA Mycobacteria v2 assay (LiPA) and/or PCR sequencing of rDNA for species-specific identification of selected MTB and all NTM isolates in Kuwait. Materials and Metho

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Robson, Jordan, and Ian Janssen. "Intensity of bouted and sporadic physical activity and the metabolic syndrome in adults." PeerJ 3 (November 26, 2015): e1437. http://dx.doi.org/10.7717/peerj.1437.

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Background.Physical activity guidelines for adults only recognize the health benefits of accumulating bouted moderate-to-vigorous physical activity (MVPA), or MVPA occurring over at least 10 consecutive minutes. There is a lack of evidence supporting the health benefits of other patterns and intensities of activity including sporadic MVPA (i.e., MVPA occurring in periods of fewer than 10 consecutive minutes) and light intensity physical activity (LIPA). The objective of this study was to examine the health benefits associated with physical activity that does not meet the physical activity guid

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Marwa, Marwa, Rahmah Muin, and Nuraimah Suharto. "Strategi Pengembangan Usaha Lipa Sa’be Mandar Di Lihat Dari Kearifan Lokal." Journal Peqguruang: Conference Series 7, no. 1 (2025): 266. https://doi.org/10.35329/jp.v6i2.5709.

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Tujuan Penelitian Ini Adalah Berdaasarkan Rumusan Masalah Diatas Penelitian Ini Mempunyai Tujuan Untuk Mengetahui Strategi Pengembangan Usaha Lipa Sa’be Mandar Yang Terjadi Berbasis Kearifan Lokal. Penelitian ini menggunakan metode kualitatif deskripti sumber data yang digunakan yaitu sumber data primer dan sekunder. Pengumpulan data diperoleh dari wawancara, observasi dan dokumentasi.Hasil penelitian yaitu Strategi pengembangan usaha lipa sa’be Mandar meliputi menjaga kualitas produk, memodifikasi produk, dan memperbanyak pilihan motif serta warna. Harga jual lipa sa’be Mandar ditentukan berd

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Niesters, H. G. M., F. Zoulim, C. Pichoud, et al. "Validation of the INNO-LiPA HBV DR Assay (Version 2) in Monitoring Hepatitis B Virus-Infected Patients Receiving Nucleoside Analog Treatment." Antimicrobial Agents and Chemotherapy 54, no. 3 (2010): 1283–89. http://dx.doi.org/10.1128/aac.00970-09.

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ABSTRACT Hepatitis B virus (HBV) antiviral drug resistance mutations prevent successful outcome of treatment and lead to worsening of liver disease. Detection of its emergence permits opportune treatment with alternative drugs. Unfortunately, the use of newly approved antivirals, including adefovir dipivoxil, emtricitabine, and telbivudine, is also associated with the development of drug resistance, albeit to a lesser extent than the use of lamivudine. The objectives of this work were to assess the performance characteristics (sensitivity and accuracy) of an updated drug resistance test, the I

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Ekblom-Bak, Elin, Örjan Ekblom, Kate A. Bolam, Björn Ekblom, Göran Bergström, and Mats Börjesson. "SCAPIS Pilot Study: Sitness, Fitness and Fatness— Is Sedentary Time Substitution by Physical Activity Equally Important for Everyone’s Markers of Glucose Regulation?" Journal of Physical Activity and Health 13, no. 7 (2016): 697–703. http://dx.doi.org/10.1123/jpah.2015-0611.

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Background:Although moderate-to-vigorous physical activity (MVPA) is mainly recommended for glucose control, light physical activity (LIPA) may also have the potential to induce favorable changes. We investigated sedentary time (SED) substitution with equal time in LIPA and MVPA, and the association with markers of glucose regulation and insulin sensitivity after stratification by waist circumference, fitness and fasting glucose levels.Methods:A total of 654 men and women, 50 to 64 years, from the SCAPIS pilot study were included. Daily SED, LIPA and MVPA were assessed using hip-worn accelerom

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Permana, Anita H., Fida Madayanti Warganegara, Deana Wahyuningrum, Made Puspasari Widhiastuty, and Akhmaloka Akhmaloka. "Heterologous Expression and Characterization of Thermostable Lipases from Geobacillus thermoleovorans PPD2 through Escherichia coli." Biosciences, Biotechnology Research Asia 14, no. 3 (2017): 1081–88. http://dx.doi.org/10.13005/bbra/2545.

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ABSTRACT: Heterologous expression and purification of thermostable lipase from Geobacillus thermoleovorans PPD2 had been carried out through Escherichia coli BL21 as host. Two bands obtained showed lipolytic activity with the size at around 51 (LipA) and 43 (LipB) kDa, respectively. The activities were identified by zymogram analysis, while the control protein from Escherichia coli BL21(DE3) do not show any lipolytic activity. Purification of crude extract using chromatography affinity Ni-NTA resulted one dominant band of LipA, meanwhile LipB did not appeared on the gel. Another purification f

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Каменец, Е. А., Н. Л. Печатникова, В. С. Какаулина, et al. "Lysosome acid lipase deficiency in Russian patients: molecular characteristic and epydemiology." Nauchno-prakticheskii zhurnal «Medicinskaia genetika», no. 8() (August 30, 2019): 3–16. http://dx.doi.org/10.25557/2073-7998.2019.08.3-16.

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Введение. Дефицит лизосомной кислой липазы (ДЛКЛ) - континуум аутосомно-рецессивно наследуемых фенотипов, обусловленных генетическими дефектами фермента лизосомной кислой липазы (ЛКЛ), играющего ключевую роль в обмене липидов. ЛКЛ кодируется геном LIPA, наиболее распространенный патогенный вариант которого -c.894G>A - является причиной заболевания в более чем половине случаев. Точная частота ДЛКЛ неизвестна, в России она предположительно составляет от 1:150 000 до 1:100 000 новорожденных. Цель: биохимический скрининг на ДЛКЛ в группе высокого риска, изучение спектра мутаций гена LIPA и оцен

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Zhao, Yu, Qian Li, Zhengfei Wang, Ziruo Dai, and Tao Chen. "Microchannel Fabrication in Fused Quartz by Backside Laser-Induced Plasma Ablation Using 248 nm KrF Excimer Laser." Applied Sciences 9, no. 24 (2019): 5320. http://dx.doi.org/10.3390/app9245320.

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Laser-induced plasma ablation (LIPA) using a 248 nm KrF excimer laser was investigated for microchannel fabrication. Examination of the morphology in relation to ablation performance was emphasized, and a synthetic LIPA mechanism model was proposed based on the results. Backside LIPA with a metal target on the bottom can be attributed to a combination of two phenomena: laser-induced plasma vaporization thermal ablation from the metal target below and enhanced laser–glass direct interaction from the plasma residuum. The laser absorption enhancement of quartz substrate resulting from the metal r

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Piran, Gerfasius Take, Hilarius Alfan, and Maria Yunita. "PATTERNS RECOGNITION (MAUMERE SARONG) USING EDGE DETECTION WITH PREWITT, SOBEL, LAPLACIAN OF GAUSSIAN (LOG), AND CANNY METHODS." Jurnal Teknik Informatika (Jutif) 5, no. 4 (2024): 1–10. https://doi.org/10.52436/1.jutif.2024.5.4.1972.

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Maumere Ikat Weaving is a cloth made from a weaving process that requires a lot of energy and time. Maumere Ikat weaving is not only limited to artistic creations; its production also considers symbols of social, religious, cultural, and economic status. The location of an image is easy if the image is clear and sharp. Still, the exact location of the edges makes it difficult to determine if the image contains interference such as noise. Objective: Recognize a Lipa pattern (Maumere Sarong) using Prewitt, Sobel, Laplacian of Gaussian (LoG), and Canny edge detection. Methods: Prewitt, Sobel, Lap

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McCarthy, Erin L., Ananda N. Rankin, Zerick R. Dill, and Squire J. Booker. "The A-type domain in Escherichia coli NfuA is required for regenerating the auxiliary [4Fe–4S] cluster in Escherichia coli lipoyl synthase." Journal of Biological Chemistry 294, no. 5 (2018): 1609–17. http://dx.doi.org/10.1074/jbc.ra118.006171.

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The lipoyl cofactor plays an integral role in several essential biological processes. The last step in its de novo biosynthetic pathway, the attachment of two sulfur atoms at C6 and C8 of an n-octanoyllysyl chain, is catalyzed by lipoyl synthase (LipA), a member of the radical SAM superfamily. In addition to the [4Fe–4S] cluster common to all radical SAM enzymes, LipA contains a second [4Fe–4S] auxiliary cluster, which is sacrificed during catalysis to supply the requisite sulfur atoms, rendering the protein inactive for further turnovers. Recently, it was shown that the Fe–S cluster carrier p

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Kaizu, Yoichi, Takeaki Kasuga, Yu Takahashi, Tomohiro Otani, and Kazuhiro Miyata. "Sleep Should Be Focused on When Analyzing Physical Activity in Hospitalized Older Adults after Trunk and Lower Extremity Fractures—A Pilot Study." Healthcare 10, no. 8 (2022): 1429. http://dx.doi.org/10.3390/healthcare10081429.

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Although the importance of resting in bed for hospitalized older adults is known, current methods of interpreting physical activity (PA) recommend the use of a broad definition of sedentary behavior (SB) that includes 0–1.5 metabolic equivalents (METs) of sleep (SL) and sitting. We investigated the characteristics of PA by conducting a cross-sectional study of 25 older adults with trunk and lower extremity fractures. The intensity of their PA was interpreted as SL (0–0.9 METs), SB (1–1.5 METs), low-intensity PA (LIPA: 1.6–2.9 METs), and moderate-to-vigorous PA (MVPA: >3.0 METs). We calculat

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Grant, Dale, David Tomlinson, Kostas Tsintzas, Petra Kolic, and Gladys Onambele-Pearson. "Displacing Sedentary Behaviour with Light Intensity Physical Activity Spontaneously Alters Habitual Macronutrient Intake and Enhances Dietary Quality in Older Females." Nutrients 12, no. 8 (2020): 2431. http://dx.doi.org/10.3390/nu12082431.

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Displacing Sedentary Behaviour (SB) with light intensity physical activity (LIPA) is increasingly viewed as a viable means of health enhancement. It is, however, unclear whether any behavioural compensations accompany such an intervention. Therefore, the aim of this study was to identify any dietary changes that accompany SB displacement. We hypothesised that SB displacement would improve dietary quality. Thirty-five elderly females (73 ± 5 years) were randomly allocated to one of three groups: (1) sedentary behaviour fragmentation (SBF) (n = 14), (2) continuous LIPA (n = 14), or (3) control (

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Liu, Zhuoliu, Luwei Fu, Maojun Pan, and Zhiwei Zhao. "Lightweight Path Recovery in IPv6 Internet-of-Things Systems." Electronics 11, no. 8 (2022): 1220. http://dx.doi.org/10.3390/electronics11081220.

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In an Internet-of-Things system supported by Internet Protocol version 6 (IPv6), the Routing Protocol for Low-Power and Lossy Networks (RPL) presents extensive applications in various network scenarios. In these novel scenarios characterized by the access of massive devices, path recovery, which reconstructs the complete path of the packet transmission, plays a vital role in network measurement, topology inference, and information security. This paper proposes a Lightweight Path recovery algorithm (LiPa) for multi-hop point-to-point communication. The core idea of LiPa is to make full use of t

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Barwais, Faisal Awad, Thomas F. Cuddihy, Tracy Washington, L. Michaud Tomson, and Eric Brymer. "Development and Validation of a New Self-Report Instrument for Measuring Sedentary Behaviors and Light-Intensity Physical Activity in Adults." Journal of Physical Activity and Health 11, no. 6 (2014): 1097–104. http://dx.doi.org/10.1123/jpah.2012-0118.

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Background:Low levels of physical activity and high levels of sedentary behavior (SB) are major public health concerns. This study was designed to develop and validate the 7-day Sedentary (S) and Light Intensity Physical Activity (LIPA) Log (7-day SLIPA Log), a self-report measure of specific daily behaviors.Method:To develop the log, 62 specific SB and LIPA behaviors were chosen from the Compendium of Physical Activities. Face-to-face interviews were conducted with 32 sedentary volunteers to identify domains and behaviors of SB and LIPA. To validate the log, a further 22 sedentary adults were

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Martin, Natalia, Esteban Lombardía, Silvia G. Altabe, Diego de Mendoza, and María C. Mansilla. "A lipA (yutB) Mutant, Encoding Lipoic Acid Synthase, Provides Insight into the Interplay between Branched-Chain and Unsaturated Fatty Acid Biosynthesis in Bacillus subtilis." Journal of Bacteriology 191, no. 24 (2009): 7447–55. http://dx.doi.org/10.1128/jb.01160-09.

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ABSTRACT Lipoic acid is an essential cofactor required for the function of key metabolic pathways in most organisms. We report the characterization of a Bacillus subtilis mutant obtained by disruption of the lipA (yutB) gene, which encodes lipoyl synthase (LipA), the enzyme that catalyzes the final step in the de novo biosynthesis of this cofactor. The function of lipA was inferred from the results of genetic and physiological experiments, and this study investigated its role in B. subtilis fatty acid metabolism. Interrupting lipoate-dependent reactions strongly inhibits growth in minimal medi

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Viswanadhapalli, Suryavathi, Xihui Liu, Uday Pratap, et al. "Abstract P6-10-14: Lysosomal acid lipase (LIPA) as a novel therapeutic vulnerability for treating TNBC." Cancer Research 83, no. 5_Supplement (2023): P6–10–14—P6–10–14. http://dx.doi.org/10.1158/1538-7445.sabcs22-p6-10-14.

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Abstract Background: TNBCs have the highest mortality rate among all BC subtypes. There is thus an urgent and unmet need for effective targeted therapies in TNBC. Recently we, identified a novel agent ERX-41 that showed good efficacy in treating TNBC in preclinical mouse models, however, its molecular action remain unknown. In this study, we identified LIPA as novel molecular target of ERX-41. Methods: We have used CRISPR knockout pooled library and multiple TNBC models for identifying molecular target of ERX-41. Mechanistic studies were performed using LIPA mutants, RNA-seq, Turbo-ID mapping,

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Rahayu, Aida Puji, Melfi Snae, and Stepanus Bani. "ETNOMATEMATIKA PADA KAIN TENUN LIPA KAET." MEGA: Jurnal Pendidikan Matematika 1, no. 1 (2020): 16–24. http://dx.doi.org/10.59098/mega.v1i1.178.

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The Indonesian education curriculum requires cultural involvement in learning process, with the aim that students can become generation that have character to preserve culture as the foundation of national character. Mathematical studies in culture are known as ethnomathematics. Ethnomathematics raises cultural wisdom as a mathematics learning resource that can motivate students in the learning process. One of the ethnomathematics objects is woven fabric. The various woven fabric motifs from each region are related to mathematics. One of them is the motif of Lipa Kaet fabric which is originati

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Salameh, Moh'd A., and Juergen Wiegel. "Purification and Characterization of Two Highly Thermophilic Alkaline Lipases from Thermosyntropha lipolytica." Applied and Environmental Microbiology 73, no. 23 (2007): 7725–31. http://dx.doi.org/10.1128/aem.01509-07.

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ABSTRACT Two thermostable lipases were isolated and characterized from Thermosyntropha lipolytica DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium which grows syntrophically with methanogens on lipids such as olive oil, utilizing only the liberated fatty acid moieties but not the glycerol. Lipases LipA and LipB were purified from culture supernatants to gel electrophoretic homogeneity by ammonium sulfate precipitation and hydrophobic interaction column chromatography. The apparent molecular masses of LipA and LipB determined by sodium dodecyl sulfate-polyacrylamide gel electrop

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Setya, Wahyu, Syamsidah Syamsidah, and Kurniati Kurniati. "Memadukan Kain Tenun Bira ( Lipa’ So’bi ) dan Kain Tenun Kajang (Tope’ Le’leng ) dalam Pembuatan Busana Casual Menggunakan Hiasan Makrame." HomeEC 19, no. 2 (2024): 36. https://doi.org/10.59562/homeec.v19i2.68251.

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ABSTRAK –Penelitian ini merupakan penelitian eksperimen yang bertujuan untuk mengetahui : 1) Desain padu padan kain tenun Kajang ( tope’ le’leng) dan kain tenun Bira (lipa’ so’bi’), 2) Proses pembuatan hiasan makrame pada padu padan kain tenun Kajang ( tope’ le’leng) dan kain tenun Bira (lipa’ so’bi’) dalam pembuatan busana casual dengan hiasan makrame 3) Proses padu padan kain tenun Kajang ( tope’ le’leng) dan kain tenun Bira (lipa’ so’bi’) dalam pembuatan busana casual dengan hiasan makrame, 4) Penilaian panelis terhadap padu padan kain tenun dalam pembuatan busana casual . Penelitian ini di

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van Doorn, Leen-Jan, Yvette J. Debets-Ossenkopp, Armelle Marais, et al. "Rapid Detection, by PCR and Reverse Hybridization, of Mutations in the Helicobacter pylori 23S rRNA Gene, Associated with Macrolide Resistance." Antimicrobial Agents and Chemotherapy 43, no. 7 (1999): 1779–82. http://dx.doi.org/10.1128/aac.43.7.1779.

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ABSTRACT A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations, associated with macrolide resistance in Helicobacter pylori. Fifty-seven H. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by PCR-LiPA (detecting mutations A2115→G, G2141→A, A2142→G, A2142→C, A2143→G, A2143→C, and A2143→T), DNA sequencing, restriction fragment length polymorphism, and/or hybridization to oligonucleotide probes.

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Collier, Alexia B., Suryavathi Viswanadhapalli, Rahul Gopalam, et al. "Novel LIPA-Targeted Therapy for Treating Ovarian Cancer." Cancers 16, no. 3 (2024): 500. http://dx.doi.org/10.3390/cancers16030500.

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Ovarian cancer (OCa) is the most lethal form of gynecologic cancer, and the tumor heterogeneities at the molecular, cellular, and tissue levels fuel tumor resistance to standard therapies and pose a substantial clinical challenge. Here, we tested the hypothesis that the heightened basal endoplasmic reticulum stress (ERS) observed in OCa represents an exploitable vulnerability and may overcome tumor heterogeneity. Our recent studies identified LIPA as a novel target to induce ERS in cancer cells using the small molecule ERX-41. However, the role of LIPA and theutility of ERX-41 to treat OCa rem

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Monroy-Pérez, Eric, Gloria Luz Paniagua-Contreras, Pamela Rodríguez-Purata, et al. "High Virulence and Antifungal Resistance in Clinical Strains ofCandida albicans." Canadian Journal of Infectious Diseases and Medical Microbiology 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/5930489.

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Antifungal resistance and virulence properties ofCandida albicansare a growing health problem worldwide. To study the expression of virulence and azole resistance genes in 39 clinical strains ofC. albicans, we used a model of infection of human vaginal epithelial cells withC. albicansstrains isolated from Mexican women with vulvovaginal candidiasis (VVC). The strains were identified by PCR amplification of the ITS1 and ITS2 regions of rRNA. The detection and expression of virulence genes and azole resistance genesMDR1andCDR1were performed using PCR and RT-PCR, respectively. All strains were se

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