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1

Zhao, Jianming 1972. "Classification and identification of yeasts by Fourier transform infrared spectroscopy." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=31564.

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Infrared spectra of microbial cells are highly specific, fingerprint-like signatures which can be used to differentiate microbial species and strains from each other. In this study, the potential applicability of Fourier transform infrared (FTIR) spectroscopy for the classification of yeast strains in terms of their biological taxonomy, their use in the production of wine, beer, and bread, and their sensitivity to killer yeast strains was investigated. Sample preparation, spectral data preprocessing methods and spectral classification techniques were also investigated. All yeast strains were g
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2

SAKALAR, Cagri. "Roles of H2A.z in Fission Yeast Chromatin." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1195137345841-32085.

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Covalent histone modifications such as methylation, acetylation as well as differential incorporation of histone variants are shown to coincide with different chromatin compartments and mark active or repressed genes. Msc1 is one of the seven JmjC Domain Proteins (JDPs) in Fission Yeast. JDPs are known to function in chromatin and some act as histone demethylases. We found that Msc1 is a member of Swr1 Complex which is known to exchange histone H2A variant H2A.z in nucleosomes. We purified H2A.z as a member of Swr1 Complex and its interaction with Swr1 Complex depends on Swr1. We’ve shown that
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3

Åslund, Ingrid, Samo Lasič, Agnieszka Nowacka, Markus Nilsson, and Daniel Topgaard. "Measuring molecular exchange for water in a yeast cell suspension through NMR diffusometry." Diffusion fundamentals 11 (2009) 69, S. 1-2, 2009. https://ul.qucosa.de/id/qucosa%3A14035.

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4

Proszynski, Tomasz. "Protein sorting and cell surface polarity in yeast." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1131974045019-73555.

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The studies presented here were focused on the understanding of the principles for protein sorting from the Golgi to the cell surface. As a marker protein we used Fus1p, a type I plasma membrane protein that is O-glycosylated on the extracellular domain and plays a role in cell fusion during yeast mating. Additionally, we analyzed mechanisms responsible for asymmetric distribution of Fus1p in mating cells. We demonstrated that the glycans attached to the protein act as a sorting determinant for protein transport to the cell surface. In cells lacking PMT4, encoding a mannosyltransferase involve
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5

Deumer, Claudia D. "RNA-binding proteins in yeast mitochondria." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2002. http://nbn-resolving.de/urn:nbn:de:swb:14-1035897639531-83407.

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This work focused on the further characterisation of Idhp and of the Krebs cycle enzymes citrate synthase 1 (Cit1p) and malate dehydrogenase 1 (Mdh1p) both of which have been identified as RNA-binding proteins without known RNA recognition motifs. Besides analysing their effects on mitochondrial translation and their organisation in protein complexes the work focused on the characterisation of the RNA-binding properties of recombinant Cit1p and Mdh1p: · Cit1p and Mdh1p play no essential role in mitochondrial protein synthesis. · Idhp is in a complex of molecular weight larger than the cytochro
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6

Nguyen, Tien Cuong, Thi Tuyen Do, Thi Hien Trang Nguyen, and Dinh Thi Quyen. "Expression, purification and evaluation of recombinant L-asparaginase inmehthylotrophic yeast Pichia pastoris: Expression, purification and evaluation of recombinant L-asparaginase in mehthylotrophic yeast Pichia pastoris: Research article." Technische Universität Dresden, 2014. https://tud.qucosa.de/id/qucosa%3A29113.

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L-asparaginase (EC 3.5.1.1), a therapeutic enzyme used in the treatment of childhood acute lymphoblastic leukemia (ALL). Hence, the goal of this work is study the expression and evaluation of hydrolysis activity of native sequence (X12746) encoding for L-asparaginase from Erwinia chrysanthemi NCPBB1125 in the popular expression system Pichia pastoris. The sequence of asn encoded for mature protein was expressed in P. pastoris SMD1168 and X33. SDS-PAGE analysis showed recombinant L-asparaginase was secreted efficiently. Stable and high hydrolysis activity of extracellular L-asparaginase in P. p
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7

Krause, Udo. "Protein phosphorylation in yeast mitochondria: enzymes, substrates and function." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-123158.

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Protein phosphorylation is one of the major post-translational modifications to allow for signal transmission and fine tuning of metabolism on the cellular proteomic level. As such it is “one of the last instances” to modulate the activity of enzymes and hence to impact the cellular life irrespective of the basic conditions provided by the genome – and epigenome– controlled gene expression. The evolutionary increase in cellular complexity is reflected by highly sophisticated regulatory networks in multicellular eukaryotes based on the transfer of phosphate mostly onto the side chains of serine
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8

Khalimonchuk, Oleh. "Yeast mitochondrial copper metabolism: topology and role of Cox11p." Doctoral thesis, Technische Universität Dresden, 2005. https://tud.qucosa.de/id/qucosa%3A24646.

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Cytochrome c oxidase (COX) is one of two known Cu-containing enzymes in mitochondria. Delivery and insertion of copper into COX are very complex processes that require multiple steps and involve a large number of assisting factors. One of the involved components is Cox11p, a copper binding protein in the inner mitochondrial membrane that is conserved from prokaryotes to eukaryotes. Cox11p is essential for respiratory growth and implicated in the assembly of the CuB site located in subunit Cox1p of COX. In the thesis the topology of Cox11p was determined and evidence for its association with th
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9

Hennig, Stefan, Gerhard Rödel, and Kai Ostermann. "Hydrophobin-Based Surface Engineering for Sensitive and Robust Quantification of Yeast Pheromones." Multidisciplinary Digital Publishing Institute (MDPI), 2016. https://tud.qucosa.de/id/qucosa%3A30004.

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Detection and quantification of small peptides, such as yeast pheromones, are often challenging. We developed a highly sensitive and robust affinity-assay for the quantification of the α-factor pheromone of Saccharomyces cerevisiae based on recombinant hydrophobins. These small, amphipathic proteins self-assemble into highly stable monolayers at hydrophilic-hydrophobic interfaces. Upon functionalization of solid supports with a combination of hydrophobins either lacking or exposing the α-factor, pheromone-specific antibodies were bound to the surface. Increasing concentrations of the pheromone
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10

Klemm, Robin. "Lipid rafts in protein sorting and yeast cell polarity." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1184754346185-43377.

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The major sorting station of biosynthetic material destined for the cell surface or secretion is the trans Golgi Network, TGN. This organelle sorts proteins and lipids into vesicular transport carriers that are targeted via different pathways to distinct membrane compartments of the cell. The molecular principles that operate in cargo sorting at the TGN are still not very well understood. Especially, we know very little about the sorting of lipids. It was postulated that a sorting mechanism based on clustering of lipid rafts, dynamic membrane domains enriched in sphingolipids and sterols, coul
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11

Demmel, Lars. "Unraveling Phosphatidylinositol 4-kinase function in the yeast Golgi-endosomal system." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1126694538661-76719.

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In Saccharomyces cerevisiae, experiments with temperature-sensitive mutants of the PI4-kinase Pik1p revealed that the PI4P pool generated by this enzyme is essential for Golgi morphology and normal secretory function and that the PI4P pool at the Golgi represents a regulatory signal on its own. In order to function as a spatial and temporal regulator of membrane traffic, PI4P synthesis and turnover must be tightly regulated. It remains elusive which factors are involved in the targeting and regulation of Pik1p. Little is also known about PI4P binding proteins mediating the effects of this phos
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12

Parshyna, Iryna. "Autophagic degradation of peroxisomes in the alkane-assimilating yeast Yarrowia lipolytica." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2006. http://nbn-resolving.de/urn:nbn:de:swb:14-1164836301920-35300.

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The thesis is aimed at understanding of molecular mechanisms of autophagic degradation of peroxisomes (pexophagy) in the yeast Yarrowia lipolytica. This microorganism has been extensively used to explore peroxisome biogenesis (Titorenko and Rachubinski, 2000). Gunkel et al. (1999) intoduced Y. lypolitica into pexophagy studies. However, the field of pexophagic research on this yeast remains quite unexplored. This work involved following tasks: (1) the development and optimization of Y. lipolytica as a model system to study peroxisome degradation; (2) Y. lipolytica genes and proteins implicated
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13

Gravert, Maike. "Novel regulators of trafficking in the yeast Golgi-endosomal system." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2006. http://nbn-resolving.de/urn:nbn:de:swb:14-1160402018221-35061.

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Over the past few years a large amount of work has provided growing insight into the molecular mechanisms that direct post-Golgi trafficking events in the budding yeast Saccharomyces cerevisae. However, a key event in this process, the formation of secretory vesicles at the Golgi and sorting of cargo into these transport carriers, remains poorly understood. It has been demonstrated that phosphatidylinositol 4-phosphate (PI(4)P) generated by the PI(4)-kinase Pik1p plays an essential role in maintenance of Golgi secretory function and morphology. Up to now relatively few targets of Pik1/PI(4)P s
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14

Jahn, Michael, Annett Mölle, Gerhard Rödel, and Kai Ostermann. "Temporal and Spatial Properties of a Yeast Multi-Cellular Amplification System Based on Signal Molecule Diffusion." MDPI, 2013. https://tud.qucosa.de/id/qucosa%3A27426.

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We report on the spatial and temporal signaling properties of a yeast pheromone-based cell communication and amplifier system. It utilizes the Saccharomyces cerevisiae mating response pathway and relies on diffusion of the pheromone α–factor as key signaling molecule between two cell types. One cell type represents the α–factor secreting sensor part and the other the reporter part emitting fluorescence upon activation. Although multi-cellular signaling systems promise higher specificity and modularity, the complex interaction of the cells makes prediction of sensor performance difficult. To te
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15

Schuller, Astrid, Gerhard Rödel, and Kai Ostermann. "Tuning the Sensitivity of the PDR5 Promoter-Based Detection of Diclofenac in Yeast Biosensors." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-230652.

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The commonly used drug diclofenac is an important environmental anthropogenic pollutant. Currently, detection of diclofenac is mainly based on chemical and physical methods. Here we describe a yeast biosensor that drives the diclofenac-dependent expression of a recombinant fluorescent protein from the authentic promoter of the PDR5 gene. This key component of the pleiotropic drug response encodes a multidrug transporter that is involved in cellular detoxification. We analyse the effects on diclofenac sensitivity of artificial PDR5 promoter derivatives in wild-type and various yeast mutant stra
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16

Gloor, Yvonne. "The Arf GTPase exchange factor Sec7p interaction network:: unraveling the crosstalk between key regulators of Golgi transport." Doctoral thesis, Technische Universität Dresden, 2007. https://tud.qucosa.de/id/qucosa%3A23740.

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The Golgi apparatus is the main crossroad of the intracellular trafficking network in all eukaryotic cells and plays a crucial role in the distribution of cellular material. To ensure the proper sorting and delivery of cargo proteins to their destination while maintaining Golgi homeostasis the coordination of all transport events to and from this organelle is required. Although a cascade of activation events has already been reported for Golgi Ypt/Rab proteins that function in the exocytic pathway, their connection to incoming vesicles from endosomal compartments or to the different Arf mediat
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17

Kronekova, Zuzana. "Assembly of mitochondrial ubiquinol-cytochrome c oxidoreductase complex in yeast Saccharomyces cerevisiae: The role of Cbp3p and Cbp4p assembly factors." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1122027648324-54732.

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Ubiquinol-cytochrome c reductase (complex III) is a central component of the respiratory chain of the inner mitochondrial membrane. It transfers electrons from reduced ubiquinone to ferricytochrome c. Correctly assembled and functional complex III is an essential prerequisite for oxidative energy metabolism. Complex III deficiency has been reported to be associated with several neurodegenerative diseases. Formation and assembly of complex III requires a multitude of specific nuclearly encoded proteins. For example, gene specific translational activators for cytochrome b synthesis as well as th
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18

Walther, Thomas. "Mathematical and Experimental Investigation of Yeast Colony Development – A Model System for the Growth of Filamentous Fungi in Heterogeneous Environments." Doctoral thesis, [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973133570.

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19

Gey, Uta. "Identifizierung und funktionelle Charakterisierung mitochondrialer Proteinkinasen und-phosphatasen in Saccharomyces cerevisiae." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-101364.

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Über die Proteinphosphorylierung in den Mitochondrien der Hefe Saccharomyces cerevisiae ist im Gegensatz zu anderen Kompartimenten nur wenig bekannt. Insbesondere hinsichtlich der physiologischen Bedeutung sowie den an der Modifikation beteiligten Enzymen sind kaum Daten verfügbar. Vor diesem Hintergrund stand die Identifizierung und molekularbiologische Charakterisierung mitochondrialer Proteinkinasen (PKasen) und Proteinphosphatasen (PPasen) im Fokus dieser Arbeit. Unter Verwendung komparativer 2D DIGE-Analysen konnten zwei Strategien erfolgreich verfolgt werden: Zum einen wurde d
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20

Krause-Buchholz, Udo. "Translationsaktivatoren der mitochondrialen Cytochrom b-Synthese in Saccaromyces cerevisiae: Membranassoziation, Mutagenese und Protein-Wechselwirkungen von Cbs1p." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2000. http://nbn-resolving.de/urn:nbn:de:swb:14-991052844578-08818.

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Die vorliegende Arbeit beschäftigt sich mit Cbs1p und Cbs2p, zwei spezifische Translationsaktivatoren der COB-mRNA. Im Mittelpunkt standen sowohl die weitere molekularbiologische und biochemische Charakterisierung von Cbs1p als auch ein Screening von Interaktionskandidaten, die mit Cbs1p und/oder Cbs2p physikalisch wechselwirken könnten. Cbs1p liegt als peripheres Membranprotein fest mit der inneren Mitochondrienmembran matrixseitig assoziiert vor. Dabei spielen möglicherweise hydrophobe und/oder Protein-Protein-Wechselwirkungen mit integralen Membranproteinen eine essentielle Rolle bei der Me
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Herzel, Lydia. "Co-transcriptional splicing in two yeasts." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-179274.

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Cellular function and physiology are largely established through regulated gene expression. The first step in gene expression, transcription of the genomic DNA into RNA, is a process that is highly aligned at the levels of initiation, elongation and termination. In eukaryotes, protein-coding genes are exclusively transcribed by RNA polymerase II (Pol II). Upon transcription of the first 15-20 nucleotides (nt), the emerging nascent RNA 5’ end is modified with a 7-methylguanosyl cap. This is one of several RNA modifications and processing steps that take place during transcription, i.e. co-trans
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Mikoliunaite, Lina, Raul D. Rodriguez, Evgeniya Sheremet, et al. "The substrate matters in the Raman spectroscopy analysis of cells." Universitätsbibliothek Chemnitz, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-178060.

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Raman spectroscopy is a powerful analytical method that allows deposited and/or immobilized cells to be evaluated without complex sample preparation or labeling. However, a main limitation of Raman spectroscopy in cell analysis is the extremely weak Raman intensity that results in low signal to noise ratios. Therefore, it is important to seize any opportunity that increases the intensity of the Raman signal and to understand whether and how the signal enhancement changes with respect to the substrate used. Our experimental results show clear differences in the spectroscopic response from cells
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Lauterbach, Alexander [Verfasser], Rudi F. [Akademischer Betreuer] Vogel, Ralf [Gutachter] Kölling, and Rudi F. [Gutachter] Vogel. "Molecular classification of yeasts of the genus Saccharomyces with respect to their application potential / Alexander Lauterbach ; Gutachter: Ralf Kölling, Rudi F. Vogel ; Betreuer: Rudi F. Vogel." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1186889519/34.

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Büchl, Nicole R., Mareike Wenning, Siegfried Scherer, and Henriette Mietke-Hofmann. "Hefendifferenzierung aus Futtermitteln." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-76155.

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Das Projekt hat zum Inhalt, Hefen aus Futtermitteln sicher mittels Fourier-Transform-Infrarot (FT-IR)-Spektroskopie identifizieren zu können. Die am Zentralinstitut für Ernährungs- und Lebensmittelforschung der TU München bestehende Datenbank zur Identifizierung von Hefen mittels FT-IR-Spektroskopie konnte durch eine Datenerweiterung für die Futtermittelmikrobiologie nutzbar gemacht werden. So wurde die sichere Differenzierung der naheverwandten Arten der Gattungen Issatchenkia und Pichia möglich, die einen wesentlichen Anteil an der Gesamthefeflora von Futtermitteln pflanzlichen Ursprungs aus
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Mayer, Jürgen. "Investigation of the biophysical basis for cell organelle morphology." Master's thesis, Max-Planck-Institut für Molekulare Zellbiologie und Genetik, 2008. https://tud.qucosa.de/id/qucosa%3A25225.

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It is known that fission yeast Schizosaccharomyces pombe maintains its nuclear envelope during mitosis and it undergoes an interesting shape change during cell division - from a spherical via an ellipsoidal and a peanut-like to a dumb-bell shape. However, the biomechanical system behind this amazing transformation is still not understood. What we know is, that the shape must change due to forces acting on the membrane surrounding the nucleus and the microtubule based mitotic spindle is thought to play a key role. To estimate the locations and directions of the forces, the shape of the nucleus
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Souza, Simone Felizardo Rocha de. "Onicomicoses causadas por fungos filamentosos não dermatófitos." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/5/5133/tde-14042008-140737/.

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INTRODUÇÃO: Onicomicose, infecção das unhas por fungo é a mais freqüente das doenças ungueais, constituindo aproximadamente metade de todas as alterações ungueais. Pode ser causada por dermatófitos, leveduras ou fungos filamentosos não dermatófitos. OBJETIVO: Caracterizar as onicomicoses causadas por fungos filamentosos não dermatófitos. (1) Verificar, dentre as suspeitas clínicas de onicomicose, qual a freqüência da recuperação de fungos,(2) Verificar, dentre as suspeitas clínicas de onicomicose, quais as espécies de fungos recuperadas, (3) Verificar, dentre o total das espécies identificadas
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Chang, Hao-Hsiang, and 張浩翔. "Segmentation and Classification of Yeast Cell." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/72668638493038122408.

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碩士<br>國立中正大學<br>資訊工程研究所<br>104<br>In this paper, we present a fully automatic segmentation and classification method to help biologist process large-scale analysis on yeast cell images. Our proposed method consists of two major parts, namely cell segmentation and cell classification respectively. In the segmentation part, we detect the seeds of yeast cells and background using Histogram of Oriented Gradients and top-hat transformation, then we perform watershed segmentation based on these seed points. In the classification part, we indicate the yeast cell with different category by generic fou
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Mitra, Aniruddha. "Sidestepping mechanism of yeast kinesin-8, Kip3." Doctoral thesis, 2017. https://tud.qucosa.de/id/qucosa%3A30820.

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Kinesin-8 motors regulate the lengths of microtubules in cells. In previous studies, these motors have been shown to utilize their highly processive plus-end directed motility to reach microtubule plus-ends where they act as a microtubule depolymerase. The superprocessive motility importantly allows Kip3 motors to depolymerize microtubules in a length-dependent manner, the underlying mechanism of which has been described by an antenna model. During such long runs, motors in vivo are expected to frequently encounter roadblocks, such as microtubule associated proteins. The adaptions in the stepp
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Ananthanarayanan, Vaishnavi. "Dynein dynamics during meiotic nuclear oscillations of fission yeast." Doctoral thesis, 2013. https://tud.qucosa.de/id/qucosa%3A27665.

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Cytoplasmic dynein is a ubiquitous minus-end directed motor protein that is essential for a variety of cellular processes ranging from cargo transport to spindle and chromosome positioning. Specifically, in fission yeast during meiotic prophase, the fused nucleus follows the spindle pole body in oscillatory movements from one cell pole to the other. The three molecular players that are essential to this process are: (i) the motor protein dynein, which powers the movement of the nucleus, (ii) microtubules, which provide the tracts for the movement and (iii) Num1, the anchor protein of dynein at
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Breen-Norris, James O., Bernard Siow, Ben Hipwell, et al. "Using Diffusion-Diffusion Exchange Spectroscopy to observe diffusion exchange in yeast." 2019. https://ul.qucosa.de/id/qucosa%3A37832.

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The permeability of cell membranes varies significantly across both healthy and diseased tissue, and changes in cell membrane permeability can occur during treatment response in tumours. Measurements of cell membrane permeability could therefore be useful for tumour detection and as biomarkers of treatment response in the clinic. As the diffusion of water across the cell membrane is directly dependent on cell membrane permeability, we have investigated the ability of diffusion-diffusion exchange spectroscopy to quantify the diffusion exchange of water in a suspension of yeast, as a first step
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Huang, Wenwen. "Theoretical Study of Pulled Polymer Loops as a Model for Fission Yeast Chromosome." Doctoral thesis, 2017. https://tud.qucosa.de/id/qucosa%3A29744.

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In this thesis, we study the physics of the pulled polymer loops motivated by a biological problem of chromosome alignment during meiosis in fission yeast. During prophase I of meiotic fission yeast, the chromosomes form a loop structure by binding their telomeres to the Spindle Pole Body (SPB). SPB nucleates the growth of microtubules in the cytoplasm. Molecular motors attached to the cell membrane can exert the force on the microtubules and thus pull the whole nucleus. The nucleus performs oscillatory motion from one to the other end of the elongated zygote cell. Experimental evidence sugges
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Groß, Annett. "Genetically Tailored Yeast Strains for Cell-based Biosensors in White Biotechnology." Doctoral thesis, 2011. https://tud.qucosa.de/id/qucosa%3A25889.

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This work was performed in the framework of two application-oriented research projects that focus on the generation and evaluation of fluorescent Saccharomyces (S.) cerevisiae-based sensor and reporter cells for white biotechnology as well as the extension of the conventional single-cell/single-construct principle of ordinary yeast biosensor approaches. Numerous products are currently generated by biotechnological processes which require continuous and precise process control and monitoring. These demands are only partially met by physical or physiochemical sensors since they measure parameter
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Korkmaz, Nuriye. "Self-assembly and Structure Investigation of Recombinant S-layer Proteins Expressed in Yeast for Nanobiotechnological Applications: Self-assembly and Structure Investigation of Recombinant S-layer Proteins Expressed in Yeast for Nanobiotechnological Applications." Doctoral thesis, 2010. https://tud.qucosa.de/id/qucosa%3A25484.

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In numerous Gram-negative and Gram-positive bacteria as well as in Archaea SL proteins form the outermost layer of the cell envelope. SL (glyco)monomers self-assemble with oblique (p2), tetragonal (p4), or hexagonal (p3, p6) symmetries [12]. SL subunits interact with each other and with the underlying cell surface by relatively weak non-covalent forces such as hydrogen-bonds, ionic bonds, salt-bridges or hydrophobic interactions. This makes them easy to isolate by applying chaotropic agents like urea and guanidine hydrochloride (GuHCl), chelating chemicals, or by changing the pH of the environ
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Hennig, Stefan. "Utilization of yeast pheromones and hydrophobin-based surface engineering for novel whole-cell sensor applications." Doctoral thesis, 2016. https://tud.qucosa.de/id/qucosa%3A29235.

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Whole-cell sensors represent an emerging branch in biosensor development since they obviate the need for enzyme/antibody purification and provide the unique opportunity to assess global parameters such as genotoxicity and bioavailability. Yeast species such as Saccharomyces cerevisiae are ideal hosts for whole-cell sensor applications. However, current approaches almost exclusively rely on analyte-induced expression of fluorescent proteins or luciferases that imply issues with light scattering and/or require the supply of additional substrates. In this study, the yeast α-factor mating pheromon
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Winters, Lora. "Mechanism of spindle assembly in Schizosaccharomyces pombe-: The role of microtubule pivoting in spindle assembly." Doctoral thesis, 2016. https://tud.qucosa.de/id/qucosa%3A30354.

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At the onset of cell division microtubules growing from spindle pole bodies (SPB) interact with each other to form the mitotic spindle enabling proper chromosome positioning and segregation. However, the exact mechanism of microtubule dynamics and microtubule associated proteins (MAPs) underlying spindle assembly is still not well understood. We developed an in vivo method to observe spindle assembly in the fission yeast Schizosaccharomyces pombe by inducing depolymerization of already formed and grown spindles by subjecting the cells to low temperatures, followed by subsequent repolymerizatio
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Coelho, Miguel. "Ageing by passive aggregation and stochastic distribution of protein aggregates." Doctoral thesis, 2011. https://tud.qucosa.de/id/qucosa%3A25912.

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In this work we report a new mechanism for ageing, where passive aggregation and stochastic segregation of protein aggregates can switch cells from a non-ageing to an ageing state. This switch is activated by the increase in the total amount of protein aggregates. We established a damage reporter system by labeling Hsp104, a chaperone which binds protein aggregates, with GFP. By observing that the accumulation of Hsp104 labeled aggregates correlated with the majority of cell death in the population, and that cells which are born with a high level of aggregates are more likely to die, we valid
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Shehata, Awad Ali. "Truncated Sequences of Influenza Subtype H5 Haemagglutinin for Vaccination and Diagnostic Purposes: Avian influenza, Yeast expression, Peptide vaccination, recombinant Elisa." Doctoral thesis, 2010. https://ul.qucosa.de/id/qucosa%3A11152.

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The highly pathogenic Avian Influenza subtype H5N1 can lead to 100 % mortality in chickens. The main issue in prevention of H5N1 is the development of efficient poultry vaccines. Influenza haemagglutinin (HA) derived recombinant polypeptides would not elicit an immune response against internal viral proteins. Thus HA polypeptide use facilitates differentiation between infected and vaccinated animals (DIVA). Serological tests using recombinant immune-dominant proteins devoid of non-specific moieties present in whole cell preparations might have higher sensitivity and specificity. In the present
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Marini, Guendalina. "High-resolution insights into macromolecular assembly: a yeast’s survival strategy." Doctoral thesis, 2017. https://tud.qucosa.de/id/qucosa%3A30960.

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Cells grow in environments that can change suddenly. To cope with unpredictable perturbations, they have evolved mechanisms to adjust their metabolism according to the various types of environmental stress. Cells experiencing starvation, for example, have low energy levels and are forced to lower their metabolism and enter a protective quiescent state to survive until nutrients become available again. Recently, it has been shown that starved yeast cells experience a marked acidification of the cytoplasm, due to a passive influx of protons. This pH drop causes multiple rearrangements in the c
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Wolf, Sylvi. "Konstruktion und Charakterisierung von Reportergenassays in S. cerevisiae und S. pombe zum Nachweis anaboler androgener Substanzen." Doctoral thesis, 2011. https://tud.qucosa.de/id/qucosa%3A26054.

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Der Missbrauch anaboler Substanzen zur Leistungssteigerung wird von den meisten Sportverbänden, dem Olympischen Komitee und vor allem der Welt Anti Doping Agentur (WADA) abgelehnt und sanktioniert. Trotzdessen bleibt der Missbrauch derartiger Substanzen sowohl im Leistungs- als auch im Freizeitsport nicht zuletzt aufgrund der starken Nebenwirkungen ein schwerwiegendes Problem. Allein im Leistungssport sind 2009 ca. 2 % der von der WADA durchgeführten Dopingkontrollen positiv ausgefallen. Dabei konnten in 65 % der positiv-getesteten Proben muskelaufbauende anabole Substanzen nachgewiesen werden
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Kövesi, Zsolt. "Biotechnological production of value-added chemicals from cis-aconitate with the help of genetically engineered oleophilic yeasts." 2020. https://tud.qucosa.de/id/qucosa%3A72926.

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Hintergrund: Die Synthese von Chemikalien aus fossilen Rohstoffen wird wegen ihrer begrenzten Verfügbarkeit und ihren negativen Auswirkungen auf die Umwelt zunehmend kritisch bewertet. Eine Alternative bietet die „Weiße Biotechnologie“, insbesondere die Fermentation nachwachsender Rohstoffe mithilfe von Hefen. Die oleophilen Hefen Pseudozyma (P.) tsukubaensis und Yarrowia (Y.) lipolytica sind natürliche Säureproduzenten. Ihre Hauptprodukte sind Metabolite des Tricarbonsäurezyklus: Citrat (CA), α-Ketoglutarat und Malat. In kleineren Mengen werden auch andere Stoffe wie Isocitrat (ICA) oder Itac
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Lauffer, Heidemarie Susann. "Charakterisierung der mitochondrialen Außenmembranproteine Om14p und Om45p von Saccharomyces cerevisiae." Doctoral thesis, 2012. https://tud.qucosa.de/id/qucosa%3A27016.

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Aufgrund der vielfältigen metabolischen Prozesse und Funktionen von Mitochondrien finden durch beide mitochondriale Membranen zahlreiche Transportprozesse statt. Es wird weitgehend angenommen, dass der Transfer von metabolischen Intermediaten durch die äußere Membran von den zahlreichen Porinporen gewährleistet wird. Im Gegensatz dazu sind in der inneren Membran spezifische Transportproteine für die Translokationsprozesse verantwortlich. Neben dem gut untersuchten Porinmolekül (Por1p) gibt es in der Hefe S. cerevisiae unter respiratorischen Bedingungen zwei weitere abundante, aber funktionell
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Oswald, Annelie. "Hairy switches and oscillators - reconstructing the zebrafish segmentation clock." Doctoral thesis, 2013. https://tud.qucosa.de/id/qucosa%3A28010.

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Formation of segments during vertebrate embryogenesis is regulated by a biological clock. Models and experimental data indicate that the core of this clock consists of a cell- autonomous single cell oscillator. This oscillator likely involves a genetic feedback loop of transcriptional repressors belonging to the hairy gene family. In zebrafish, three her genes, her1, hes6 and her7, have been identified as core oscillator components. The main purpose of this project was to study the molecular mechanism of the hairy gene negative feedback oscillator in single cells. To determine whether a singl
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Altenkirch, Falko. "Alternative Auslesemöglichkeiten für Hefe-Ganzzellsensoren." 2018. https://tud.qucosa.de/id/qucosa%3A32740.

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Ganzzellsensoren sind potentielle Kandidaten für den Einsatz in der Umwelttechnik zur Detektion von Schwermetallen, organischen Lösungsmitteln oder Xenobiotika. Ebenso können mit ihrer Hilfe andauernde Prozesse, wie z.B. in der Biogasentwicklung, überwacht werden. Etablierte, auf Genexpression basierende Ausleseverfahren besitzen unterschiedliche Nachteile, die den Einsatz bisher weitestgehend auf das Labor beschränken. In der vorliegenden Arbeit wurden dazu drei alternative Auslesemöglichkeiten evaluiert, die durch kostengünstige Messverfahren und einem einfachen experimentellen Aufbau realis
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Schuster, Linda. "Entfernung von β-Lactam- und Makrolid-Antibiotika aus Wässern mit Hilfe von gentechnisch modifizierten Saccharomyces cerevisiae-Zellen". 2020. https://tud.qucosa.de/id/qucosa%3A73096.

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Antibiotika sind für die Behandlung von bakteriellen Infektionskrankheiten in der Human- und Veterinärmedizin von immenser Bedeutung. Angesichts der Korrelation zwischen Antibiotika-Einsatzmengen und der Häufigkeit resistenter Organismen ist eine unsachgemäße bzw. übermäßige Verwendung dieser antibakteriellen Wirkstoffe sowie deren Eintrag über die Kläranlagen in die Umwelt äußerst problematisch. Neben Vermeidungs- und Verminderungsstrategien besteht ein Ansatz zur Problemlösung in der Entwicklung innovativer Technologien zur Entfernung von Antibiotikarückständen aus Wässern, da konventionelle
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Ansel, Elisabeth. "Ein irischer 3. Mai? Jack B. Yeats’ Funeral of Harry Boland (1922) im Kontext der Stilkonstruktion einer irischen Moderne." 2019. https://tud.qucosa.de/id/qucosa%3A35262.

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Mitte der 1920er Jahre und im Kontext der irischen Unabhängigkeit erklärte der Kunstkritiker Thomas MacGreevy (1893–1967) den Maler Jack B. Yeats (1871–1957) zum Nationalkünstler Irlands und verglich sein Werk mit keinem Geringeren als dem Francisco de Goyas. Im Zuge dessen konstatierte er, „Yeats’ […] pictures such as A Republican Funeral will [one day] be considered as important a part of the nation’s history as Goya’s 3rd of May is to the Spanish art lover” (MacGreevy 7999/1, S. 8). Bemerkenswert ist dabei zunächst, dass MacGreevy die historischen Gegebenheiten im Spanien Goyas mit denen Ir
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