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Journal articles on the topic 'Yeast one-hybrid assay'

Author: Grafiati

Published: 4 June 2021

Last updated: 7 February 2022

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1

Okoye, Mercy E., Gerry L. Sexton, Eugene Huang, J. Michael McCaffery, and Prashant Desai. "Functional Analysis of the Triplex Proteins (VP19C and VP23) of Herpes Simplex Virus Type 1." Journal of Virology 80, no. 2 (2006): 929–40. http://dx.doi.org/10.1128/jvi.80.2.929-940.2006.

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ABSTRACT The triplex of herpesvirus capsids is a unique structural element. In herpes simplex virus type 1 (HSV-1), one molecule of VP19C and two of VP23 form a three-pronged structure that acts to stabilize the capsid shell through interactions with adjacent VP5 molecules. The interaction between VP19C and VP23 was inferred by yeast cryoelectron microscopy studies and subsequently confirmed by the two-hybrid assay. In order to define the functional domains of VP19C and VP23, a Tn7-based transposon was used to randomly insert 15 bp into the coding regions of these two proteins. The mutants wer

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2

Schulten, Hans-Jürgen, Wolfgang Engel, Karim Nayernia, and Peter Burfeind. "Yeast One-Hybrid Assay Identifies YY1 as a Binding Factor for a Proacrosin Promoter Element." Biochemical and Biophysical Research Communications 257, no. 3 (1999): 871–73. http://dx.doi.org/10.1006/bbrc.1999.0556.

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Chen, Gang, and Jumi A. Shin. "AhR/Arnt:XRE interaction: Turning false negatives into true positives in the modified yeast one-hybrid assay." Analytical Biochemistry 382, no. 2 (2008): 101–6. http://dx.doi.org/10.1016/j.ab.2008.07.026.

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Evans, David R. H., Kendra A. Swirsding, Bruce E. Taillon, and Jan F. Simons. "“One plate/three-reporter” assay format for the detection and validation of yeast two-hybrid interactions." BioTechniques 37, no. 5 (2004): 840–43. http://dx.doi.org/10.2144/04375pt02.

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5

Zhou, Zhong-xun, Bin He, Susan H. Hall, Elizabeth M. Wilson, and Frank S. French. "Domain Interactions between Coregulator ARA70 and the Androgen Receptor (AR)." Molecular Endocrinology 16, no. 2 (2002): 287–300. http://dx.doi.org/10.1210/mend.16.2.0765.

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Abstract The coregulator function of AR-associated protein 70 (ARA70) was investigated to further characterize its interaction with the AR. Using a yeast two-hybrid assay, androgen-dependent binding of ARA70 deletion mutants to the AR ligand-binding domain (LBD) was strongest with ARA70 amino acids 321–441 of the 614 amino acid ARA70 protein. Mutations adjacent to or within an FxxLF motif in this 120-amino acid region abolished androgen-dependent binding to the AR-LBD both in yeast and in glutathione-S-transferase affinity matrix assays. Yeast one-hybrid assays revealed an intrinsic ARA70 tran

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Wang, Dan, Changyue Jiang, Wandi Liu, and Yuejin Wang. "The WRKY53 transcription factor enhances stilbene synthesis and disease resistance by interacting with MYB14 and MYB15 in Chinese wild grape." Journal of Experimental Botany 71, no. 10 (2020): 3211–26. http://dx.doi.org/10.1093/jxb/eraa097.

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Abstract Resveratrol is notable not only for its functions in disease resistance in plants but also for its health benefits when it forms part of the human diet. Identification of new transcription factors helps to reveal the regulatory mechanisms of stilbene synthesis. Here, the WRKY53 transcription factor was isolated from the Chinese wild grape, Vitis quinquangularis. Vqwrky53 was expressed in a variety of tissues and responded to powdery mildew infection and to exogenous hormone application. VqWRKY53 was located in the nucleus and had transcriptional activation activity in yeast. A yeast t

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Saglam, Nihal Goren. "Identification of promoter DNA/protein interactions for MYB transcription factor in senescence using Yeast-One-Hybrid assay." Journal of Biotechnology 231 (August 2016): S20—S21. http://dx.doi.org/10.1016/j.jbiotec.2016.05.095.

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8

Huang, Kai, Tao Wu, Ziming Ma, et al. "Rice Transcription Factor OsWRKY55 Is Involved in the Drought Response and Regulation of Plant Growth." International Journal of Molecular Sciences 22, no. 9 (2021): 4337. http://dx.doi.org/10.3390/ijms22094337.

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WRKY transcription factors (TFs) have been reported to respond to biotic and abiotic stresses and regulate plant growth and development. However, the molecular mechanisms of WRKY TFs involved in drought stress and regulating plant height in rice remain largely unknown. In this study, we found that transgenic rice lines overexpressing OsWRKY55 (OsWRKY55-OE) exhibited reduced drought resistance. The OsWRKY55-OE lines showed faster water loss and greater accumulation of hydrogen peroxide (H2O2) and superoxide radical (O2−·) compared to wild-type (WT) plants under drought conditions. OsWRKY55 was

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Tang, Xuan, Junwei Shi, and Wubei Dong. "A yeast library-hybrid assay to screen maize- Rhizoctonia transcription factors and protein-protein interactions in one experimental pipeline." Agri Gene 1 (August 2016): 15–22. http://dx.doi.org/10.1016/j.aggene.2016.05.002.

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10

Schwartz, Katja, Kristy Richards, and David Botstein. "BIM1Encodes a Microtubule-binding Protein in Yeast." Molecular Biology of the Cell 8, no. 12 (1997): 2677–91. http://dx.doi.org/10.1091/mbc.8.12.2677.

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A previously uncharacterized yeast gene (YER016w) that we have named BIM1 (binding to microtubules) was obtained from a two-hybrid screen of a yeast cDNA library using as bait the entire coding sequence of TUB1 (encoding α-tubulin). Deletion of BIM1 results in a strong bilateral karyogamy defect, hypersensitivity to benomyl, and aberrant spindle behavior, all phenotypes associated with mutations affecting microtubules in yeast, and inviability at extreme temperatures (i.e., ≥37°C or ≤14°C). Overexpression of BIM1 in wild-type cells is lethal. A fusion of Bim1p with green fluorescent protein th

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WANG, QIANJIE, WEIWEI QI, YING WANG, et al. "Isolation and identification of an AP2/ERF factor that binds an allelic cis-element of rice gene LRK6." Genetics Research 93, no. 5 (2011): 319–32. http://dx.doi.org/10.1017/s0016672311000218.

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SummaryAllelic expression of the rice yield-related gene, leucine-rich receptor-like kinase 6 (LRK6), in the hybrid of 93-11 (Oryza sativa L. subsp. Indica var. 93-11) and Nipponbare (O. sativa L. subsp. Japonica var. Nipponbare) is determined by allelic promoter cis-elements. Using deletion analysis of the LRK6 promoter, we identified two distinct regions that might contribute to LRK6 expression. Sequence alignment revealed differences in these LRK6 promoter regions in 93-11 and Nipponbare. One of the segments, named differential sequence of LRK6 promoter 2 (DSLP2), contains potential transcr

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Clark, Sean W., and Mark D. Rose. "Arp10p Is a Pointed-End-associated Component of Yeast Dynactin." Molecular Biology of the Cell 17, no. 2 (2006): 738–48. http://dx.doi.org/10.1091/mbc.e05-05-0449.

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In metazoans, dynein-dependent vesicle transport is mediated by dynactin, containing an actin-related protein, Arp1p, together with a cargo-selection complex containing a second actin-related protein, Arp11. Paradoxically, in budding yeast, models of dynactin function imply an interaction with membranes, whereas the lack of microtubule-based vesicle transport implies the absence of a cargo-selection complex. Using both genetic and biochemical approaches, we demonstrate that Arp10p is the functional yeast homologue of Arp11, suggesting the possible existence of a pointed-end complex in yeast. S

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Khu, Yee-Ling, Esther Koh, Siew Pheng Lim, et al. "Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase." Journal of Virology 75, no. 1 (2001): 205–14. http://dx.doi.org/10.1128/jvi.75.1.205-214.2001.

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ABSTRACT Interaction between viral proteins is necessary for viral replication and viral particle assembly. We used the yeast two-hybrid assay to identify interactions among all the mature proteins of the hepatitis C virus. The interaction between NS3 and NS3 was one of the strongest viral protein-protein interactions detected. The minimal region required for this interaction was mapped to a specific subdomain of 174 amino acids in the N terminus of the helicase region. Random mutations in the minimal region were generated by PCR, and mutants that failed to interact with a wild-type minimal fr

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Qi, Qu, Gao, et al. "The Heterologous Expression of a Chrysanthemum nankingense TCP Transcription Factor Blocks Cell Division in Yeast and Arabidopsis thaliana." International Journal of Molecular Sciences 20, no. 19 (2019): 4848. http://dx.doi.org/10.3390/ijms20194848.

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Both the presence of, and the important contribution to growth and development made by TCP transcription factors, have been established in various plant species. Here, a TCP4 homolog isolated from Chrysanthemum nankingense was shown to be more strongly transcribed in the diploid than in the autotetraploid form of the species. CnTCP4 was shown to encode a member of the class II TCP family and to be transcribed most strongly in the leaf and ligulate flowers. Its transcription was found to be substantially inhibited by spraying the plant with the synthetic cytokinin 6-benzylaminopurine. The trans

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15

Haney, Steven A., Elizabeth Glasfeld, Cynthia Hale, David Keeney, Zhizhen He, and Piet de Boer. "Genetic Analysis of theEscherichia coliFtsZ·ZipA Interaction in the Yeast Two-hybrid System." Journal of Biological Chemistry 276, no. 15 (2001): 11980–87. http://dx.doi.org/10.1074/jbc.m009810200.

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The recruitment of ZipA to the septum by FtsZ is an early, essential step in cell division inEscherichia coli. We have used polymerase chain reaction-mediated random mutagenesis in the yeast two-hybrid system to analyze this interaction and have identified residues within a highly conserved sequence at the C terminus of FtsZ as the ZipA binding site. A search for suppressors of a mutation that causes a loss of interaction (ftsZD373G) identified eight different changes at two residues within this sequence.In vitro, wild type FtsZ interacted with ZipA with a high affinity in an enzyme-linked imm

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Guo, Fen, Yue-Qin Li, Shi-Qian Li, et al. "Interaction of Mouse Pem Protein and Cell Division Cycle 37 Homolog." Acta Biochimica et Biophysica Sinica 37, no. 11 (2005): 784–87. http://dx.doi.org/10.1111/j.1745-7270.2005.00102.x.

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Abstract Mouse Pem, a homeobox gene, encodes a protein consisting of 210 amino acid residues. To study the function of mouse Pem protein, we used the yeast two-hybrid system to screen the library of 7-day mouse embryo with full-length mouse Pem cDNA. Fifty-two colonies were obtained after 1.57×108 colonies were screened by nutrition limitation and β-galactosidase assay. Seven individual insert fragments were obtained from the library, and three of them were identified, one of which was confirmed to be the cell division cycle 37 (Cdc37) homolog gene by sequencing. The interaction between mouse

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17

Piron, Maria, Thierry Delaunay, Jeanne Grosclaude, and Didier Poncet. "Identification of the RNA-Binding, Dimerization, and eIF4GI-Binding Domains of Rotavirus Nonstructural Protein NSP3." Journal of Virology 73, no. 7 (1999): 5411–21. http://dx.doi.org/10.1128/jvi.73.7.5411-5421.1999.

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ABSTRACT The rotavirus nonstructural protein NSP3 is a sequence-specific RNA binding protein that binds the nonpolyadenylated 3′ end of the rotavirus mRNAs. NSP3 also interacts with the translation initiation factor eIF4GI and competes with the poly(A) binding protein. Deletion mutations and point mutations of NSP3 from group A rotavirus (NSP3A), expressed in Escherichia coli, indicate that the RNA binding domain lies between amino acids 4 and 149. Similar results were obtained with NSP3 from group C rotaviruses. Data also indicate that a dimer of NSP3A binds one molecule of RNA and that dimer

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18

Krajčíková, Daniela, Magda Lukáčová, Denisa Müllerová, Simon M. Cutting, and Imrich Barák. "Searching for Protein-Protein Interactions within the Bacillus subtilis Spore Coat." Journal of Bacteriology 191, no. 10 (2009): 3212–19. http://dx.doi.org/10.1128/jb.01807-08.

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ABSTRACT The capability of endospores of Bacillus subtilis to withstand extreme environmental conditions is secured by several attributes. One of them, the protein shell that encases the spore and is known as the coat, provides the spore with its characteristic resistance to toxic chemicals, lytic enzymes, and predation by unicellular and multicellular eukaryotes. Despite most of the components of the spore coat having been identified, we have only a vague understanding of how such a complex structure is assembled. Using the yeast two-hybrid system, we attempted to identify direct contacts amo

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Hussain, Amjad, Clement Wesley, Mohammad Khalid, Ashutosh Chaudhry, and Shahid Jameel. "Human Immunodeficiency Virus Type 1 Vpu Protein Interacts with CD74 and Modulates Major Histocompatibility Complex Class II Presentation." Journal of Virology 82, no. 2 (2007): 893–902. http://dx.doi.org/10.1128/jvi.01373-07.

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ABSTRACT The human immunodeficiency virus type 1 (HIV-1) Vpu accessory protein is a transmembrane protein that down regulates CD4 expression and promotes the release of new virions. We screened a human leukocyte-specific yeast two-hybrid expression library to discover novel Vpu-interacting cellular proteins. The major histocompatibility complex class II (MHC II) invariant chain, also called Ii or CD74, was found to be one such protein. We show direct binding of Vpu and CD74 by using a yeast two-hybrid assay and coimmunoprecipitation from HIV-1-infected cells. The cytoplasmic region of Vpu was

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20

Zhang, Nenggang, Sergey G. Kuznetsov, Shyam K. Sharan, Kaiyi Li, Pulivarthi H. Rao, and Debananda Pati. "A handcuff model for the cohesin complex." Journal of Cell Biology 183, no. 6 (2008): 1019–31. http://dx.doi.org/10.1083/jcb.200801157.

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The cohesin complex is responsible for the accurate separation of sister chromatids into two daughter cells. Several models for the cohesin complex have been proposed, but the one-ring embrace model currently predominates the field. However, the static configuration of the embrace model is not flexible enough for cohesins to perform their functions during DNA replication, transcription, and DNA repair. We used coimmunoprecipitation, a protein fragment complement assay, and a yeast two-hybrid assay to analyze the protein–protein interactions among cohesin subunits. The results show that three o

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Li, Xiao-Dong, Tomi P. Mäkelä, Deyin Guo, et al. "Hantavirus nucleocapsid protein interacts with the Fas-mediated apoptosis enhancer Daxx." Journal of General Virology 83, no. 4 (2002): 759–66. http://dx.doi.org/10.1099/0022-1317-83-4-759.

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Hantaviruses cause two severe diseases, haemorrhagic fever with renal syndrome in Eurasia and hantavirus pulmonary syndrome in the Americas. To understand more about the molecular mechanisms that lead to these diseases, the associations of Puumala virus nucleocapsid protein (PUUV-N) with cellular proteins were studied by yeast two-hybrid screening. Daxx, known as an apoptosis enhancer, was identified from a HeLa cDNA library and its interaction with PUUV-N was confirmed by GST pull-down assay, co-immunoprecipitation and co-localization studies. Furthermore, domains of interaction were mapped t

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WANG, Guozheng, Shu ZHANG, David G. FERNIG, et al. "Heterodimeric interaction and interfaces of S100A1 and S100P." Biochemical Journal 382, no. 1 (2004): 375–83. http://dx.doi.org/10.1042/bj20040142.

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With the widespread use of yeast two-hybrid systems, many heterodimeric forms of S100 proteins have been found, although their biological significance is unknown. In the present study, S100A1 was found to interact with another S100 protein, S100P, by using the yeast two-hybrid system. The binding parameters of the interaction were obtained using an optical biosensor and show that S100P has a slightly higher affinity for S100A1 (Kd=10–20 nM) when compared with that for self-association (Kd=40–120 nM). The physical interaction of S100A1 and S100P was also demonstrated in living mammalian cells u

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Yao, Jun, Zedan Shen, Yanli Zhang, et al. "Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance." Journal of Experimental Botany 71, no. 4 (2019): 1527–39. http://dx.doi.org/10.1093/jxb/erz493.

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Abstract Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter–reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 prote

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Li, Xieyu, Ting Wu, Hanting Liu, et al. "REVEILLE Transcription Factors Contribute to the Nighttime Accumulation of Anthocyanins in ‘Red Zaosu’ (Pyrus Bretschneideri Rehd.) Pear Fruit Skin." International Journal of Molecular Sciences 21, no. 5 (2020): 1634. http://dx.doi.org/10.3390/ijms21051634.

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Anthocyanin biosynthesis exhibits a rhythmic oscillation pattern in some plants. To investigate the correlation between the oscillatory regulatory network and anthocyanin biosynthesis in pear, the anthocyanin accumulation and the expression patterns of anthocyanin late biosynthetic genes (ALBGs) were investigated in fruit skin of ‘Red Zaosu’ (Pyrus bretschneideri Rehd.). The anthocyanin accumulated mainly during the night over three continuous days in the fruit skin, and the ALBGs’ expression patterns in ‘Red Zaosu’ fruit skin were oscillatory. However, the expression levels of typical anthocy

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Yeakley, Joanne M., Hélène Tronchère, James Olesen, Jacqueline A. Dyck, Huan-You Wang, and Xiang-Dong Fu. "Phosphorylation Regulates In Vivo Interaction and Molecular Targeting of Serine/Arginine-rich Pre-mRNA Splicing Factors." Journal of Cell Biology 145, no. 3 (1999): 447–55. http://dx.doi.org/10.1083/jcb.145.3.447.

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The SR superfamily of splicing factors and regulators is characterized by arginine/serine (RS)-rich domains, which are extensively modified by phosphorylation in cells. In vitro binding studies revealed that RS domain–mediated protein interactions can be differentially affected by phosphorylation. Taking advantage of the single nonessential SR protein–specific kinase Sky1p in Saccharomyces cerevisiae, we investigated RS domain interactions in vivo using the two-hybrid assay. Strikingly, all RS domain–mediated interactions were abolished by SKY1 deletion and were rescuable by yeast or mammalian

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Gammie, Alison E., and Naz Erdeniz. "Characterization of Pathogenic Human MSH2 Missense Mutations Using Yeast as a Model System: A Laboratory Course in Molecular Biology." Cell Biology Education 3, no. 1 (2004): 31–48. http://dx.doi.org/10.1187/cbe.03-08-0006.

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This work describes the project for an advanced undergraduate laboratory course in cell and molecular biology. One objective of the course is to teach students a variety of cellular and molecular techniques while conducting original research. A second objective is to provide instruction in science writing and data presentation by requiring comprehensive laboratory reports modeled on the primary literature. The project for the course focuses on a gene, MSH2, implicated in the most common form of inherited colorectal cancer. Msh2 is important for maintaining the fidelity of genetic material wher

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Li, Xiangyi, Lei He, Xiaohui An, et al. "VviWRKY40, a WRKY Transcription Factor, Regulates Glycosylated Monoterpenoid Production by VviGT14 in Grape Berry." Genes 11, no. 5 (2020): 485. http://dx.doi.org/10.3390/genes11050485.

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Glycosylated volatile precursors are important, particularly in wine grape berries, as they contribute to the final aroma in wines by releasing volatile aglycones during yeast fermentation and wine storage. Previous study demonstrated that VviGT14 was functioned as a critical monoterpene glucosyltransferase in grape berry, while the transcriptional regulation mechanism of VviGT14 was still unknown. Here we identified VviWRKY40 as a binding factor of VviGT14 promoter by both DNA pull-down and yeast one-hybrid screening, followed by a series of in vitro verification. VviWRKY40 expression pattern

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Yang, Guangzhe, Dong Chao, Zhenhua Ming, and Jixing Xia. "A Simple Method to Detect the Inhibition of Transcription Factor-DNA Binding Due to Protein–Protein Interactions In Vivo." Genes 10, no. 9 (2019): 684. http://dx.doi.org/10.3390/genes10090684.

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Binding of transcription factors (TFs) to cis-regulatory elements (DNA) could modulate the expression of downstream genes, while interactions between TFs and other proteins might inhibit them binding to DNA. Nowadays, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) approaches are usually employed to detect the inhibitory effect. However, EMSA might not reflect the inhibitory effect in vivo. ChIP requires preparation of specific antibody or stable genetic transformation and complicated experimental steps, making it laborious and time-consuming. Here, based o

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Thiel, Heike, Kamal Hleibieh, David Gilmer, and Mark Varrelmann. "The P25 Pathogenicity Factor of Beet necrotic yellow vein virus Targets the Sugar Beet 26S Proteasome Involved in the Induction of a Hypersensitive Resistance Response via Interaction with an F-box Protein." Molecular Plant-Microbe Interactions® 25, no. 8 (2012): 1058–72. http://dx.doi.org/10.1094/mpmi-03-12-0057-r.

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P25, a Beet necrotic yellow vein virus (BNYVV) pathogenicity factor, interacts with a sugar beet protein with high homology to Arabidopsis thaliana kelch repeat containing F-box family proteins (FBK) of unknown function in yeast. FBK are members of the Skp1-Cullin-F-box (SCF) complex that mediate protein degradation. Here, we confirm this sugar beet FBK–P25 interaction in vivo and in vitro and provide evidence for in planta interaction and similar subcellular distribution in Nicotiana tabacum leaf cells. P25 even interacts with an FBK from A. thaliana, a BNYVV nonhost. FBK functional classific

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Madina, Mst Hur, Md Saifur Rahman, Xiaoqiang Huang, Yang Zhang, Huanquan Zheng, and Hugo Germain. "A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility." Biology 9, no. 9 (2020): 294. http://dx.doi.org/10.3390/biology9090294.

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Melampsora larici-populina (Mlp), the causal agent of Populus leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic Arabidopsis line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement

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Smaldone, Silvia, Friedrich Laub, Cindy Else, Cecilia Dragomir, and Francesco Ramirez. "Identification of MoKA, a Novel F-Box Protein That Modulates Krüppel-Like Transcription Factor 7 Activity." Molecular and Cellular Biology 24, no. 3 (2004): 1058–69. http://dx.doi.org/10.1128/mcb.24.3.1058-1069.2004.

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ABSTRACT KLF7, a member of the Krüppel-like transcription factor family, is believed to regulate neurogenesis and cell cycle progression. Here, a yeast two-hybrid screen for KLF7 cofactors in the developing nervous system identified a novel 140-kDa protein named MoKA, for modulator of KLF7 activity. Interaction between MoKA and KLF7 was confirmed by the in vitro glutathione S-transferase pull-down assay and by coimmunoprecipitation of the proteins overexpressed in mammalian cells. Functional assays documented that MoKA is a KLF7 coactivator, and in situ hybridizations identified the developin

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Urano, Emiko, Noriko Kuramochi, Reiko Ichikawa, et al. "Novel Postentry Inhibitor of Human Immunodeficiency Virus Type 1 Replication Screened by Yeast Membrane-Associated Two-Hybrid System." Antimicrobial Agents and Chemotherapy 55, no. 9 (2011): 4251–60. http://dx.doi.org/10.1128/aac.00299-11.

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ABSTRACTHuman immunodeficiency virus (HIV) Gag protein targets to the plasma membrane and assembles into viral particles. In the next round of infection, the mature Gag capsids disassemble during viral entry. Thus, Gag plays a central role in the HIV life cycle. Using a yeast membrane-associated two-hybrid assay based on the SOS-RAS signaling system, we developed a system to measure the Gag-Gag interaction and isolated 6 candidates for Gag assembly inhibitors from a chemical library composed of 20,000 small molecules. When tested in the human MT-4 cell line and primary peripheral blood mononuc

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Hosoda, Kenta, Yuichiro Kanno, Masashi Sato, Jun Inajima, Yoshio Inouye та Kazuyuki Yanai. "Identification of CAR/RXR<i>α</i> Hetero-dimer Binding Sites in the Human Genome by a Modified Yeast One-Hybrid Assay". Advances in Biological Chemistry 05, № 02 (2015): 83–97. http://dx.doi.org/10.4236/abc.2015.52008.

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Li, Zhenghe, Daniel Barajas, Tadas Panavas, David A. Herbst, and Peter D. Nagy. "Cdc34p Ubiquitin-Conjugating Enzyme Is a Component of the Tombusvirus Replicase Complex and Ubiquitinates p33 Replication Protein." Journal of Virology 82, no. 14 (2008): 6911–26. http://dx.doi.org/10.1128/jvi.00702-08.

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ABSTRACT To identify host proteins interacting with Tomato bushy stunt virus (TBSV) replication proteins in a genome-wide scale, we have used a yeast (Saccharomyces cerevisiae) proteome microarray carrying 4,088 purified proteins. This approach led to the identification of 58 yeast proteins that interacted with p33 replication protein. The identified host proteins included protein chaperones, ubiquitin-associated proteins, translation factors, RNA-modifying enzymes, and other proteins with yet-unknown functions. We confirmed that 19 of the identified host proteins bound to p33 in vitro or in a

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Sun, Qinfu, Jueyi Xue, Li Lin, et al. "Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L." Agronomy 8, no. 10 (2018): 222. http://dx.doi.org/10.3390/agronomy8100222.

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Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated tha

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Simcha, Inbal, Catherine Kirkpatrick, Einat Sadot та ін. "Cadherin Sequences That Inhibit β-Catenin Signaling: A Study in Yeast and Mammalian Cells". Molecular Biology of the Cell 12, № 4 (2001): 1177–88. http://dx.doi.org/10.1091/mbc.12.4.1177.

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Drosophila Armadillo and its mammalian homologue β-catenin are scaffolding proteins involved in the assembly of multiprotein complexes with diverse biological roles. They mediate adherens junction assembly, thus determining tissue architecture, and also transduce Wnt/Wingless intercellular signals, which regulate embryonic cell fates and, if inappropriately activated, contribute to tumorigenesis. To learn more about Armadillo/β-catenin's scaffolding function, we examined in detail its interaction with one of its protein targets, cadherin. We utilized two assay systems: the yeast two-hybrid sys

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Pan, Hong-Yi, Yan-Jin Zhang, Xin-Ping Wang, Jian-Hong Deng, Fu-Chun Zhou, and Shou-Jiang Gao. "Identification of a Novel Cellular Transcriptional Repressor Interacting with the Latent Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus." Journal of Virology 77, no. 18 (2003): 9758–68. http://dx.doi.org/10.1128/jvi.77.18.9758-9768.2003.

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ABSTRACT The latent nuclear antigen (LNA) of Kaposi's sarcoma-associated herpesvirus (KSHV) has an essential role in viral latent infection. LNA maintains the stability of KSHV episomes and modulates the expression of cellular genes. A novel cellular protein KLIP1 was identified to interact with LNA through yeast two-hybrid screening, and confirmed by a glutathione S-transferase pull down assay. Domain mapping showed that KLIP1 interacted with the N-terminal domain of LNA. Northern blot hybridization with a KLIP1 probe identified a major transcript of 1.8 kb and a minor transcript of 2.8 kb. c

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Li, Shaofeng, Yaoxiang Zhang, Xuebing Xin, et al. "The Osmotin-Like Protein Gene PdOLP1 Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar." International Journal of Molecular Sciences 21, no. 11 (2020): 3993. http://dx.doi.org/10.3390/ijms21113993.

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Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated PdOLP1 from Populus deltoides (Marsh.). Expression analysis showed that PdOLP1 transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in P. deltoides. We conducted phenotypic, anatomical, and molecular analyses of PdOLP1-overexpressing lines and the PdOLP1-downregulated hybrid poplar 84K (Populus alba × Populus glandulosa

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TARDIF, Marianne, Laurence BROUCHON, Marie-Josèphe RABIET, and François BOULAY. "Direct binding of a fragment of the Wiskott-Aldrich syndrome protein to the C-terminal end of the anaphylatoxin C5a receptor." Biochemical Journal 372, no. 2 (2003): 453–63. http://dx.doi.org/10.1042/bj20021803.

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Migration of myeloid cells towards a source of chemoattractant, such as the C5a anaphylatoxin, is triggered by the activation of a G-protein-coupled receptor. In the present study, we have used a yeast two-hybrid approach to find unknown partners of the C5a receptor (C5aR). Using the cytosolic C-terminal region of C5aR as bait to screen a human leucocyte cDNA library, we identified the Wiskott–Aldrich syndrome protein (WASP) as a potential partner of C5aR. WASP is known to have an essential function in regulating actin dynamics at the cell leading edge. The interaction was detected with both t

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Luan, Hexiang, Haopeng Niu, Jinyan Luo, and Haijian Zhi. "Soybean Cytochrome b5 Is a Restriction Factor for Soybean Mosaic Virus." Viruses 11, no. 6 (2019): 546. http://dx.doi.org/10.3390/v11060546.

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Soybean mosaic virus (SMV) is one of the most destructive viral diseases in soybeans (Glycine max). In this study, an interaction between the SMV P3 protein and cytochrome b5 was detected by yeast two-hybrid assay, and bimolecular fluorescence complementation assay showed that the interaction took place at the cell periphery. Further, the interaction was confirmed by co-immunoprecipitation analysis. Quantitative real-time polymerase chain reaction analysis revealed that GmCYB5 gene was differentially expressed in resistant and susceptible soybean plants after inoculation with SMV-SC15 strain.

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Lee, Eun-Gyung, та Maxine L. Linial. "Basic Residues of the Retroviral Nucleocapsid Play Different Roles in Gag-Gag and Gag-Ψ RNA Interactions". Journal of Virology 78, № 16 (2004): 8486–95. http://dx.doi.org/10.1128/jvi.78.16.8486-8495.2004.

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ABSTRACT The Orthoretrovirus Gag interaction (I) domain maps to the nucleocapsid (NC) domain in the Gag polyprotein. We used the yeast two-hybrid system to analyze the role of Alpharetrovirus NC in Gag-Gag interactions and also examined the efficiency of viral assembly and release in vivo. We could delete either or both of the two Cys-His (CH) boxes without abrogating Gag-Gag interactions. We found that as few as eight clustered basic residues, attached to the C terminus of the spacer peptide separating the capsid (CA) and NC domains in the absence of NC, was sufficient for Gag-Gag interaction

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Wang, Yawen, Shuai Zhang, Yi Tang, and Youxiang Diao. "Screening of Duck Tembusu Virus NS3 Interacting Host Proteins and Identification of Its Specific Interplay Domains." Viruses 11, no. 8 (2019): 740. http://dx.doi.org/10.3390/v11080740.

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NS3 protein is a member of the non-structural protein of duck Tembusu virus (DTMUV), which contains three domains, each of which has serine protease, nucleotide triphosphatase, and RNA helicase activities, respectively. It performs a variety of biological functions that are involved in the regulation of the viral life cycle and host immune response. Based on the yeast two-hybrid system, we successfully transformed pGBKT7-NS3 bait plasmid into Y2H Gold, tested it to prove that it has no self-activation and toxicity, and then hybridized it with the prey yeast strain of the duck embryo fibroblast

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Jin, Yong-Mei, Rihua Piao, Yong-Feng Yan, et al. "Overexpression of a New Zinc Finger Protein Transcription FactorOsCTZFP8Improves Cold Tolerance in Rice." International Journal of Genomics 2018 (2018): 1–13. http://dx.doi.org/10.1155/2018/5480617.

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Cold stress is one of the most important abiotic stresses in rice. C2H2zinc finger proteins play important roles in response to abiotic stresses in plants. In the present study, we isolated and functionally characterized a new C2H2zinc finger protein transcription factorOsCTZFP8in rice.OsCTZFP8encodes a C2H2zinc finger protein, which contains a typical zinc finger motif, as well as a potential nuclear localization signal (NLS) and a leucine-rich region (L-box). Expression ofOsCTZFP8was differentially induced by several abiotic stresses and was strongly induced by cold stress. Subcellular local

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Nguyen, Thu, Dani B. N. Vinh, Douglas K. Crawford та Trisha N. Davis. "A Genetic Analysis of Interactions with Spc110p Reveals Distinct Functions of Spc97p and Spc98p, Components of the Yeast γ-Tubulin Complex". Molecular Biology of the Cell 9, № 8 (1998): 2201–16. http://dx.doi.org/10.1091/mbc.9.8.2201.

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The spindle pole body (SPB) in Saccharomyces cerevisiae functions as the microtubule-organizing center. Spc110p is an essential structural component of the SPB and spans between the central and inner plaques of this multilamellar organelle. The amino terminus of Spc110p faces the inner plaque, the substructure from which spindle microtubules radiate. We have undertaken a synthetic lethal screen to identify mutations that enhance the phenotype of the temperature-sensitive spc110–221 allele, which encodes mutations in the amino terminus. The screen identified mutations inSPC97 and SPC98, two gen

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Heath, Victoria L., Sidney L. Shaw, Sharmili Roy, and Martha S. Cyert. "Hph1p and Hph2p, Novel Components of Calcineurin-Mediated Stress Responses in Saccharomyces cerevisiae." Eukaryotic Cell 3, no. 3 (2004): 695–704. http://dx.doi.org/10.1128/ec.3.3.695-704.2004.

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ABSTRACT Calcineurin is a Ca2+- and calmodulin-dependent protein phosphatase that plays a key role in animal and yeast physiology. In the yeast Saccharomyces cerevisiae, calcineurin is required for survival during several environmental stresses, including high concentrations of Na+, Li+, and Mn2+ ions and alkaline pH. One role of calcineurin under these conditions is to activate gene expression through its regulation of the Crz1p transcription factor. We have identified Hph1p as a novel substrate of calcineurin. HPH1 (YOR324C) and its homolog HPH2 (YAL028W) encode tail-anchored integral membra

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Gringel, Susanne, Jeroen van Bergeijk, Kirsten Haastert, Claudia Grothe, and Peter Claus. "Nuclear fibroblast growth factor-2 interacts specifically with splicing factor SF3a66." Biological Chemistry 385, no. 12 (2004): 1203–8. http://dx.doi.org/10.1515/bc.2004.156.

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Abstract Fibroblast growth factor 2 (FGF-2) has a dual role as a classical extracellular signaling protein and as an intracellular factor. Isoforms of FGF-2, resulting from alternatively used start codons on one mRNA species, locate differentially to nuclear compartments. In this study we aimed to analyze functions of intracellular FGF-2 by identification of interacting proteins. We identified the 66-kDa subunit of splicing factor 3a (SF3a66) as a binding partner in a yeast two-hybrid screen and confirmed this interaction by pull-down assays. The splicing factor interacted with the 18-kDa (FGF

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Liu, Dan, Yang-Yang Li, Zhi-Cheng Zhou, et al. "Tobacco transcription factor bHLH123 improves salt tolerance by activating NADPH oxidase NtRbohE expression." Plant Physiology 186, no. 3 (2021): 1706–20. http://dx.doi.org/10.1093/plphys/kiab176.

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Abstract In plants, reactive oxygen species (ROS) produced following the expression of the respiratory burst oxidase homolog (Rboh) gene are important regulators of stress responses. However, little is known about how plants acclimate to salt stress through the Rboh-derived ROS signaling pathway. Here, we showed that a 400-bp fragment of the tobacco (Nicotiana tabacum) NtRbohE promoter played a critical role in the salt response. Using yeast one-hybrid (Y1H) screens, NtbHLH123, a bHLH transcription factor, was identified as an upstream partner of the NtRbohE promoter. These interactions were c

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Yang, Yanli, Hui-Guang Li, Jie Wang, et al. "ABF3 enhances drought tolerance via promoting ABA-induced stomatal closure by directly regulating ADF5 in Populus euphratica." Journal of Experimental Botany 71, no. 22 (2020): 7270–85. http://dx.doi.org/10.1093/jxb/eraa383.

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Abstract Water availability is a main limiting factor for plant growth, development, and distribution throughout the world. Stomatal movement mediated by abscisic acid (ABA) is particularly important for drought adaptation, but the molecular mechanisms in trees are largely unclear. Here, we isolated an ABA-responsive element binding factor, PeABF3, in Populus euphratica. PeABF3 was preferentially expressed in the xylem and young leaves, and was induced by dehydration and ABA treatments. PeABF3 showed transactivation activity and was located in the nucleus. To study its functional mechanism in

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Yong, Zhang, and Lyu. "A Stress-Responsive NAC Transcription Factor from Tiger Lily (LlNAC2) Interacts with LlDREB1 and LlZHFD4 and Enhances Various Abiotic Stress Tolerance in Arabidopsis." International Journal of Molecular Sciences 20, no. 13 (2019): 3225. http://dx.doi.org/10.3390/ijms20133225.

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Our previous studies have indicated that a partial NAC domain protein gene is strongly up-regulated by cold stress (4 °C) in tiger lily (Lilium lancifolium). In this study, we cloned the full-length of this NAC gene, LlNAC2, to further investigate the function of LlNAC2 in response to various abiotic stresses and the possible involvement in stress tolerance of the tiger lily plant. LlNAC2 was noticeably induced by cold, drought, salt stresses, and abscisic acid (ABA) treatment. Promoter analysis showed that various stress-related cis-acting regulatory elements were located in the promoter of L

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Wulf, Anne, Marianne G. Wetzel, Maxim Kebenko, et al. "The role of thyroid hormone receptor DNA binding in negative thyroid hormone-mediated gene transcription." Journal of Molecular Endocrinology 41, no. 1 (2008): 25–34. http://dx.doi.org/10.1677/jme-08-0023.

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Thyroid hormone 3,3′,5-tri-iodothyronine (T3) regulates gene expression in a positive and negative manner. Here, we analyzed the regulation of a positively (mitochondrial glycerol-3-phosphate dehydrogenase) and negatively T3-regulated target gene (TSHα). Thyroid hormone receptor (TR) activates mGPDH but not TSH promoter fragments in a mammalian one-hybrid assay. Furthermore, we investigated functional consequences of targeting TR to DNA independent of its own DNA-binding domain (DBD). Using a chimeric fusion protein of the DBD of yeast transcription factor Gal4 with TR, we demonstrated a posit

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