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Relevant bibliographies by topics / Yeast preparation

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Academic literature on the topic 'Yeast preparation'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 February 2022

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Journal articles on the topic "Yeast preparation"

1

Simpson, R. J. "Preparation of Extracts from Yeast." Cold Spring Harbor Protocols 2011, no. 1 (January 1, 2011): pdb.prot5545. http://dx.doi.org/10.1101/pdb.prot5545.

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Jairath, S., P. Sahota, and G. Pandove. "Preparation of non-alcoholic naturally carbonated beverage using yeast isolate from whey beverage." Czech Journal of Food Sciences 30, No. 2 (March 9, 2012): 135–43. http://dx.doi.org/10.17221/248/2010-cjfs.

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Four pure yeast isolates from feta cheese whey beverage were phenotypically characterised and D1/D2 domain of 26S rRNA and Internal Transcribed Spacer (ITS) region were sequenced. These isolates were identified as Clavispora lusitaniae (84), Candida sp. YS12A (86), Clavispora lusitaniae (B82), and Candida hyderabadensis (S82). The fermentation potentials of all yeast isolates were determined in plum, amla, lemon, guava, kinnow, and pineapple, and Clavispora lusitaniae (84) was reported as the best yeast for carrying out fermentation with CO<sub>2</sub> levels of 1.5 bar. Using Clavispora lusitaniae, a reliable, controllable, simple, and reproducible technology from astringent fruits has been developed for the production of non-alcoholic naturally carbonated beverage with improved tangy taste, appearance, aroma, extended shelf life, and retention of all the nutrients. This yeast on inoculation @ 0.5% in astringent in amla juice (13%), TSS adjusted to 16.0°B, and fermentetion at 20 ± 5°C for 36 h produces a new non-alcoholic naturally carbonated beverage. The physicochemical parameters of freshly prepared beverage juice 13%, pH 3.0, TSS 16.0°B, acidity 0.38%, Brix acid ratio 42.10, ascorbic acid 120.0 mg/100 ml. The physicochemical parameters did not change significantly during storage. The volatile components like propanol, butanol, acetaldehyde, methanol, ethyl acetate, and isopropanol were found to be absent while the percentage of ethanol was 1.16% after three months of storage. Shelf life of the beverage is three months under refrigerated conditions (4°C).  

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Kovaleva, T. S., G. V. Agafonov, A. N. Yakovlev, and S. F. Yakovleva. "Effect of protease and phytase on the physiological state of alcoholic yeast in cultivation." Proceedings of the Voronezh State University of Engineering Technologies 81, no. 4 (February 11, 2020): 98–102. http://dx.doi.org/10.20914/2310-1202-2019-4-98-102.

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Saccharomyces cerevisiae yeast is used in the production of ethyl alcohol. The main requirements for yeast used in the production of ethyl alcohol from starch-containing raw materials: alcohol yeast used in the processing of starchy raw materials must have high fermentation activity; complete fermentability of sugars, resistance to metabolic products, resistance to the development of extraneous microflora. Proteolytic enzyme preparation Prolive BS Liquid was used as a source of protease. Kingphos enzyme preparation was used as a source of phytase. The effect of the enzyme preparations of the neutral protease Prolive BS Liquid and Phytase Kingphos on the fermentation activity of alcohol yeast Saccharomyces cerevisiae race XII was studied. The maximum fermentation activity is possessed by yeast cultivated on the wort, obtained using protease and phytase. The duration of the exponential growth phase in the experiment is 14–16 hours, in the control -18–20 hours. The exponential phase is described by the Mono equation. Compared to the yeast in the control, the yeast in the experiment multiplies more intensively, and by 14–16 hours of growth, about 170 million yeast cells accumulate in the culture medium, and the yeast in the control-about 95 million yeast cells by 18–20 h of growth. The specific growth rate was maximum in the logarithmic phase and amounted to 0.35 million cells / cm3 • h in the experimental samples and 0.25 million cells / cm3 • h in the control. It was found that the maximum accumulation of yeast cells was observed when the neutral enzyme Prolive BS Liquid was added to the wort with a dosage of 0.2 units. PS/g of starch and enzyme preparation Phytase Kingfos with a dosage of 0.5 units. FS/g of starch, the yeast cell content in mature yeast reached 170 million cells / cm3 by 16-18 hours of cultivation, the yeast has a high fermentation activity.

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4

Alenkina, T. V., O. S. Zinina, M. V. Antonycheva, N. I. Vakhrushina, and A. K. Nikiforov. "Optimization of Reproduction Stage in Technology of Production of Plague Diagnostic Bacteriophage L-413C." Problems of Particularly Dangerous Infections, no. 2(108) (April 20, 2011): 79–82. http://dx.doi.org/10.21055/0370-1069-2011-2(108)-79-82.

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New nutrient media based on baker yeast autolizate were used for the first time for manufacturing of diagnostic preparation of plague bacteriophage L-413C. Experimental media provide high concentration of phage particles at the stage of propagation, and good survivability in lyophilization. Media in which yeast autolizate was a nutrient protein basis appeared to be more effective than those in which it was a stimulating additive. Phage preparations preserved stability of properties during storage at 4-8 °С, and at a higher temperature in the test of accelerated aging. Introduction of yeast nutrient media in technology of plague diagnostic bacteriophage L-413C manufacturing opens good prospects for increasing of production efficiency and decreasing of cost value of the preparation

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Holdsworth, E. S., D. V. Kaufman, and E. Neville. "A fraction derived from brewer's yeast inhibits cholesterol synthesis by rat liver preparations in vitro." British Journal of Nutrition 65, no. 2 (March 1991): 285–99. http://dx.doi.org/10.1079/bjn19910087.

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Brewer's yeast was grown on a defined medium containing tracer51Cr with or without added chromium. The two batches of yeast contained 10 μg/g (high-Cr) or 80 ng/g (low-Cr). Extracts were prepared and fractionated. A third batch of yeast (third batch) was grown with added Cr, and fractionated. Rats were reared on either rat cubes (normal diet) or on a low-Cr diet (low-Cr), or on rat cubes with added cholestyramine (cholestyramine diet). Preparations of rat liver, both cell-free and intact hepatocytes, incorporated acetate-carbon into fatty acids and cholesterol. These processes were inhibited by a yeast fraction containing small, neutral, water-soluble compounds. The degree of inhibition was the same whether the liver came from normal rats or rats fed on the low-Cr diet. Similarly the inhibitory effect was found with identical amounts of extracts from low- or high-Cr yeasts. Therefore, Cr compounds do not appear to account for the inhibitory effects of brewer's yeast. Use of other substrates indicated that the site of inhibition of sterol synthesis was apparently between acetyl-CoA and mevalonate. One inhibitory substance was isolated from yeast and was found to be nicotinamide riboside. This may have been produced from NAD(P) during the preparation of yeast extracts, and it may be produced from dietary yeast supplements during digestion in vivo. Nicotinamide riboside may be partly responsible for the reported effects of yeast supplements on plasma lipids in humans.

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Schwartz, Katja, and Gavin Sherlock. "Preparation of Yeast DNA Sequencing Libraries." Cold Spring Harbor Protocols 2016, no. 10 (October 2016): pdb.prot088930. http://dx.doi.org/10.1101/pdb.prot088930.

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Layne, Elizabeth A., and Sonja Zabel. "Impression Smear Agreement with Acetate Tape Preparation for Cytologic Sampling." Journal of the American Animal Hospital Association 53, no. 4 (July 1, 2017): 193–97. http://dx.doi.org/10.5326/jaaha-ms-6458.

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ABSTRACT Cutaneous cytologic sampling techniques are used to detect bacteria, yeast, and inflammatory cells for diagnosis and therapeutic monitoring. Studies have examined slide evaluation techniques, ear swab cytology staining methods, and observer variations; few studies compare common clinical sampling techniques. The primary aim of this study was to measure detection of microorganisms and neutrophils by impression smear compared to acetate tape preparation; comparison of agreement between two acetate tape staining methods was a secondary aim. Thirty lesions consistent with superficial pyoderma were sampled via impression smear and acetate tape preparation. Acetate tape preparations were either stained with modified Romanowksy stain solutions two and three or solution three alone. Impression smears were stained in the standard manner. Bacteria, yeast, and neutrophils were evaluated using a semi-quantitative scale [0-4]. Quantities were aggregated and compared using Cohen's kappa to measure agreement between methods. When impression smears were compared to acetate tape, the lowest agreement occurred for neutrophils, with impression smears detecting more neutrophils. Comparison of acetate tape staining methods had the highest agreement for yeast detection. Sampling technique and staining method did not differ for detection of bacteria. Impression smears detected more neutrophils, and yeast detection appeared equivalent for acetate tape staining methods.

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Darmohray, L. M., B. V. Gutyj, and О. О. Darmohray. "Antimicrobic activity concept of water extract of plants Galega orientalis (Lam.)." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 87 (April 26, 2018): 122–25. http://dx.doi.org/10.15421/nvlvet8724.

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It was first conducted testing on antimicrobial activity of Galega orientalis (La) on the growth pure cultural of bacteria gram positive (Micrococcus luteus), gram negative (Escherichia coli XL1, DH5) and yeasts (Saccharomyces cerevisiae W303). The material for the study was dried vegetative mass Galega orientalis (Lam) in the phase of budding and early flowering. In experiment used medium «Endo» for gram negative, medium LB for gram positive and gram negative, and suslo agar for yeasts. As a result of the experiment was revealed that 20% concentration of aqueous extract of this plant had inhibitory effects on the growth of pure cultures of bacteria and yeast. Antimicrobial effect of this drug on the growth of gram-negative bacteria (Escherichia coli XL1, DH5) were within 20.0–30.0% (P < 0.001), gram-positive bacteria (M. Luteus) – 12.0% (P < 0.05) and yeast (S. cerevisiae W303) – 30.5% (P < 0.01) compared with control. It should be noted that in all cases the addition of the drug Galega orientalis (La) did not alter the morphology of colonies (colony size) test strains. The questions of search the new antimicrobial agents, include natural origin, is very actual during last years. Increasing microbiological pure of eating products, feed grinders, veterinarian preparations are one of the urgent task of Scientifics and industrials. It has proved the influence of different concentrations of aqueous extract of this culture on the growth of pure cultures of gram-positive and gram-negative bacteria and yeast. Install antimicrobial influence 20–30% concentrations of preparation on the bacteries growth (Escherichia coli XL1, DH5). Bacterial action of preparation on the bacteries (Micrococcus luteus) and yeasts (Saccharomyces cerevisiae W303) were lower. It was described the prospects for further investigation of this problem. It has proved possible relationship between the antimicrobial activity of the extract of this plant and bloating of the rumen in ruminants.

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Szuba-Trznadel, Anna, Tomasz Hikawczuk, Małgorzata Korzeniowska, and Bogusław Fuchs. "Dietary Supplementation of a Yeast-Whey Preparation for Weaned Piglets." Acta Veterinaria 70, no. 1 (March 1, 2020): 126–35. http://dx.doi.org/10.2478/acve-2020-0009.

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AbstractWeaning is a stressful period for the piglets and the sow. Stress during weaning is related to the change of diet which can affect the physiology of the gastrointestinal tract, as well as the microbial and immunological status of the animals. In the experiment a yeast-whey preparation was used to decrease the transient growth depression related to reduction of feed intake by the piglets. The piglets were assigned to three treatments. In the control group (I) the animals obtained standard feed mixture used routinely at the farm. In the case of piglets from II and III treatment, the yeast-whey preparation was added in the quantity of 4 and 7%, respectively.Application of 7% yeast-whey preparation to the diet significantly increased the body weight of piglets (p<0.05) and in consequence the average daily body weight gain (p<0.01) in comparison with the control group of animals. Additionally, piglets which were fed the yeast-whey preparation diet had a higher feed intake (p<0.05) and better feed conversion ratio (p<0.05) than those fed a diet without the addition of this preparation. No significant differences were stated for most biological parameters (p>0.05), except for the blood urea level, which was significantly lower (p<0.05) in the treatments where the yeast-whey preparation was used. These results indicated that yeast-whey preparation efficiently suppressed post-weaning diarrhea and improved the performance of the animals.

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Fracassetti, Daniela, Stefano Massaglia, Andrea Viberti, Giulia Motta, Roberto Foschino, and Ileana Vigentini. "Wine Industry’s Attitude towards Oenological Yeasts: Italy as a Case Study." Beverages 6, no. 2 (May 17, 2020): 33. http://dx.doi.org/10.3390/beverages6020033.

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Yeast inoculation is a widespread practice in winemaking in order to control the must fermentation. However, the use of indigenous wine yeasts can enrich wine quality and differentiate wine styles. Yeast cream preparation (CRY), recently accepted by the International Organization of Vine and Wine, could allow an easier usage of autochthonous yeasts. This work aimed at investigating the actual Italian wine industry’s attitude towards the available formulations of commercial wine yeasts with attention to CRY. Moreover, this study evaluated the perception of wineries toward indigenous yeasts in both winemaking and marketing viewpoints. Data show different levels of knowledge and use about the available yeast formulations. In general, there is not a predominantly positive or negative participants’ opinion regarding the use of indigenous yeasts. Wineries using CRY (4% of the sample) mainly adopt them as a part of the production in order to compare the wines with the ones traditionally obtained with commercial yeasts. CRY is perceived by some interviewees as a potential tool to increase communication and product differentiation. This survey could have anticipated future trends in the use of yeast formulations, determined by the market demands for diversified, unique, and environmentally sustainable products, that can allow an accessible application of precision enology.

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Dissertations / Theses on the topic "Yeast preparation"

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Guzman-Juarez, M. "Yeast protein products : Preparation, characterisation and functional properties." Thesis, University of Reading, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376661.

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Klimaitė, Jūratė. "Karvių sergančių slaptuoju mastitu diagnostika, gydymas ir profilaktika." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2005. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050926_152516-82961.

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The distribution of yeast fungi has been ascertained as their influence on SCM etiology. Our developed preparation for treatment for yeast and microbe caused mastitis “Gentafung” is composition of wide spectrum antibiotics and antifungal medications. An evaluation was performed of commercially available homeopathic preparations in treating SCM. A new recommended preparation “OrbeScal” was evaluated for drying cows. A comparison of Candida genus commercial identification systems diagnostic accuracy was via classical methods.

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TIMMERMAN, JOHANNA. "Preparation and structural study of the dna-binding domain of the cyp1 protein of the yeast saccharomyces cerevisiae." Paris 11, 1994. http://www.theses.fr/1994PA112429.

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Dans la levure saccharomyces cerevisiae, la proteine cyp1 est un regulateur de la transcription de nombreux genes dont l'expression est dependante de l'oxygene. Nous nous sommes interesse a la determination de la structure tridimensionnelle du domaine de fixation a l'adn de cette proteine. Cette partie contient un motif cys-xaa(2)-cys-xaa(6)-cys-xaa(6)-cys-xaa(2)-cys-xaa(8)-cys, que l'on retrouve dans de nombreuses proteines de levure interagissant avec l'adn, en particulier dans la proteine gal4. Le but a long terme est de determiner le complexe cyp1-adn par resonance magnetique nucleaire. Dans ce travail nous presentons le clonage et l'expression dans escherichia coli des quatre fragments differents correspondant a la partie n-terminale de l'activateur cyp1 responsable de la fixation a l'adn. Le fragment cyp1(49-126) a ete choisi pour une etude structurale par resonance magnetique nucleaire. Pour ce fragment, un protocole de purification a ete mis au point. Nous montrons ensuite que le zinc est un element essentiel de la structure de la proteine et que le fragment cyp1(49-126) possede 2 atomes de zinc. Les memes resultats ont ete observes lorsqu'on substitue deux atomes de zinc par les atomes cadmium 113. Par resonance magnetique nucleaire, nous avons demontre que les six cysteines du motif ligandent les deux atomes de cadmium. Le fragment a ete ensuite marque avec de l'azote 15. L'ensemble des experiences rmn homonucleaires a deux dimensions (cosy, tocsy et noesy) et heteronucleaires a deux et trois dimensions (hmqc, noe-hmqc, hohaha-hmqc) a permis l'attribution de la partie n-terminale de cyp1(49-126). Les donnees experimentales obtenues par ces experiences ont ete utilisees dans des programmes de modelisation, diana et x-plor. Une structure tridimensionnelle du fragment a pu etre presentee et montre que notre fragment est replie en cluster a zinc

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Merrill, Melissa L. "The ability of a yeast-derived cell wall preparation to minimize toxic effects of high-alkaloid tall fescue straw in beef cattle /." Connect to this title online, 2007. http://hdl.handle.net/1957/4044.

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Riordan, Cathal G. "Studies on brewery yeast preparations for environmental bioremediation." Thesis, University of Ulster, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274562.

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Cool, Marc. "Etude des gènes codant pour le facteur d'élongation EF-1 alpha de levure : isolement et analyse du gène TEF2, contribution à la construction de vecteur d'expression de gène hétérologue à la levure." Paris 6, 1986. http://www.theses.fr/1986PA066363.

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Brümmer, Mieke. "The effect of yeast cell wall preparations on salmonella colonisation, gastrointestinal health and performance of broiler chickens." Pretoria : [s.n.], 2007. http://upetd.up.ac.za/thesis/available/etd-04212008-112014.

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Tan, Xiao. "DISCOVERY OF SELECTIVE PROBES TARGETING RNA POLYMERASE I." UKnowledge, 2019. https://uknowledge.uky.edu/chemistry_etds/113.

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RNR Polymerase I (RNA Pol I) is a “factory” that orchestrate the transcription of ribosomal rRNA for constructing ribosomes as a primary workshop for protein translation to sustain cell growth. Misregulation of RNA Pol I can cause uncontrolled cell proliferation, which leads to the development of cancer. Yeast (Saccharomyces cerevisiae) is a valuable model system to study RNA Pol I. Recently, the X-ray crystal structure of the yeast homologue of RNA Pol I was elucidated, offering the structural basis to selectively target this transcriptional machinery. The approach to selective RNA Pol I targeting was to disrupt the interaction between a specific transcription factor, RRN3 that bind distinct regions of RNA Pol I. For this purpose, a recombined plasmid was designed to carry human rDNA plus its promoter as target together with a selection marker gene. Therefore, this plasmid could not only introduce the target gene into the yeast (host), but also facilitate the passage of this target gene into a stable yeast strain. In this project, one uracil deficient yeast strain of YBR140C was transformed with the recombined yeast integrative plasmid of pHmrDNA-YIPlac211-TG1. This is a recombined plasmid containing not only the human rDNA but also the URA3 gene as a selection marker. PCR amplification of the human ribosomal DNA was indicative of successful integration of the human ribosomal DNA into the genome of the two yeast strains. Virtual screening using a library of 700 FDA-approved compounds was docked into the RRN3-RNA Pol I complex to identify small molecule disruptors of the RRN3-RNA Pol I as a selective strategy. Using growth assays, gel electrophoresis and transcriptional assays, we identified cerivastatin sodium as a lead virtual hit. The result implicates cerivastatin sodium as a selective RNA Pol I inhibitor worthy of further development with potential as targeted anticancer therapeutic.

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Nyman, Jonas, and Michael Lacintra. "Co-cultures of Yeasts and Zygomycetes in the Form of Pellets Methods for the Preparation of Pellets and Biocapsules, Their Properties and Applications." Thesis, Högskolan i Borås, Institutionen Ingenjörshögskolan, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-17277.

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Many industrially important fungi can grow in the form of small spherical pellets.Such pellets reduce the viscosity and enhances mass transfer rates in culture broths.The pelleted morphology also influences the fungus’s metabolism, directing it tospecific metabolites. The pellets are easily harvested from the broth and recycled.These properties makes pelleted morphology very attractive.The pelleted morphology of four Zygomycetes strains was studies. Several differentnutrient media used by other researchers for achieving pelleted growth was tested.The influence of eight factors on pelletization of Rhizopus sp. in a completely definedmedium was determined using a fractional central composite design and logistic regression.Pelleted growth of all four Zygomycetes was achieved, with very good results for twoRhizomucor sp. strains. A simple medium containing calcium carbonate was foundto induce pelletization with very high reproducibility.Immobilization of yeast cells was attempted in pellets of Rhizomucor. It was foundthat a flocculating yeast can be immobilized inside pellets of fungal mycelium, forming”biocapsules”. This is accomplished by first using a medium that induces pelletizationof the filamentous fungus and does not allow for growth of the yeast. Thepellets are then inoculated into a second medium that induces growth and flocculationof the yeast and inhibits further growth of the filamentous fungus.Non-flocculating yeasts could not be immobilized, suggesting that the flocculin proteinsin the cell wall of flocculating strains are important for proper immobilization.The flocculation and immobilization arises due to expression of several differentFLO-genes and the importance of these genes for successful immobilization isdiscussed.The results demonstrate that the morphology of Zygomycetes can be controlled andthat this may be useful in industrial fermentation. The new immobilization techniquereveals the great importance of flocculation and cell surface hydrophobicity. Usingyeast strains that express certain FLO-genes may be beneficial in fermentation oflignocellulosic hydrolysates.Microscopy techniques were developed that allows for high quality microphotographyof pellets and thin cross-sections of pellets and biocapsules.
Program: MSc in Resource Recovery - Industrial Biotechnology

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Zechmeisterová, Lucie. "Faktory ovlivňující kvalitu červeného vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216207.

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In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.

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Books on the topic "Yeast preparation"

1

Riordan, Cathal G. Studies on brewery yeast preparations for environmental bioremediation. [S.l: Cathal G. Riordan, 1998.

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Book chapters on the topic "Yeast preparation"

1

Kuroda, Kouichi. "Preparation of Functional Cells: Improvement of Stress Tolerance." In Yeast Cell Surface Engineering, 85–92. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_7.

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Dunn, Elizabeth A., and Stephen D. Rader. "Preparation of Yeast Whole Cell Splicing Extract." In Methods in Molecular Biology, 123–35. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-980-2_9.

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Norbeck, Joakim. "Preparation of Yeast Samples for 2-D PAGE." In Springer Protocols Handbooks, 155–58. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_17.

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Norbeck, Joakim. "Preparation of Yeast Samples for 2-D PAGE." In The Proteomics Protocols Handbook, 27–30. Totowa, NJ: Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-890-0:027.

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Matsuyama, Akihisa, Atsuko Shirai, and Minoru Yoshida. "Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation." In Methods in Molecular Biology, 125–33. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7546-4_12.

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Fuganti, Claudio. "Baker’s Yeast Mediated Preparation of Carbohydrate-Like Chiral Synthons." In Enzymes as Catalysts in Organic Synthesis, 3–17. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4686-6_1.

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Creanor, James. "Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces pombe." In Cell Cycle — Materials and Methods, 132–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-57783-3_12.

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Carneiro, Sónia, Rui Pereira, and Isabel Rocha. "Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis." In Methods in Molecular Biology, 197–207. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0563-8_12.

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Rosén, Knut. "Preparation of Yeast for Industrial use in Production of Beverages." In Biotechnology Applications in Beverage Production, 169–87. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-1113-0_12.

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Kamisaka, Yasushi. "Preparation of Oleaginous Yeast by Genetic Modification and Its Potential Applications." In Biocatalysis and Biomolecular Engineering, 57–65. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470608524.ch4.

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Conference papers on the topic "Yeast preparation"

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Yang, Chuang, Zhichao Qiu, Yuanwei Cao, and Junling Wang. "Study on the Factors Influencing the Preparation of Iron - Zinc Yeast." In 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/meita-16.2017.101.

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Huang, Wei-Hong, Wei-Bai Bian, Hong-Xiang Ou, Yun-Lei Zhang, Xiang-Mei Weng, Jian-Ming Pan, and Yong-Sheng Yan. "Preparation of magnetic Ce3+-imprinted Chitosan/yeast composites and selective adsorption of Ce3+." In 2013 International Conference on Advanced Materials and Information Technology Processing. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/amitp130021.

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Chiselitsa, Oleg, Natalia Chiselitsa, Alina Beshliu, Elena Tofan, Nadejda Efremova, Ana Lozan, and Marina Danilis. "BIOLOGICALLY ACTIVE PROTEIN PREPARATION BASED ON YEAST BIOMASSFROM THE WASTE OF THE BEER INDUSTRY." In XIth International Congress of Geneticists and Breeders from the Republic of Moldova. Scientific Association of Geneticists and Breeders of the Republic of Moldova, Institute of Genetics, Physiology and Plant Protection, Moldova State University, 2021. http://dx.doi.org/10.53040/cga11.2021.122.

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Chijun Zhou and Quan Zhou. "Preparation and characterization of a novel sludge conditioner by Microbial flocculant (MBF) extracted from waste yeast with nano SiO2 particles." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5964338.

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Kolpakova, Valentina, Denis Kulikov, Ruzaliya Ulanova, Nikolay Lukin, and Irina Gaivoronskaya. "BIOCONVERSION OF CEREAL SERUM - A SECONDARY PRODUCT FOR PRODUCING PROTEIN CONCENTRATES FROM PEA AND CHICK PEAS." In GEOLINKS International Conference. SAIMA Consult Ltd, 2020. http://dx.doi.org/10.32008/geolinks2020/b1/v2/06.

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Studies on the bioconversion of whey water formed from chickpea and pea grains in the preparation of protein concentrates have been performed. The serum remaining after precipitation of the main part of the protein was subjected to a symbiotic transformation of Saccharomyces cerevisiae 121 and Geotrichum candidum 977 yeast cultures with the formation of protein-containing products with a mass fraction of protein (52.27-57.90% of DS) and a complementary amino acid composition. A microbial-plant concentrate was used as an additive in the feeding of Wistar laboratory rats. After 25 days of feeding, there was no negative effect on the physiological parameters and behavior of animals, which indicates the high quality of the protein product and the prospects of its inclusion in the composition of animal feed and diets.

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Karges, H. E., G. Zettlemeiβl, H. Naumann, U. Eberhard, and M. Bröker. "PURIFICATION AND CHARACTERIZATION OF GENTECHNOLOGICALLY PREPARED ANTITHROMBIN III." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643684.

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Isolation and purification of antithrombin III (AT III) by affinity chromatography on immobilized heparin is a standard method for the large scale preparation of this protein from human or animal plasma. Hence, after AT III became available by gentechnological methods, we tried to adapt this procedure for the isolation of AT III from supernatants of mammalian- and yeast-cells. Indeed, it was possible to use this method also for the isolation of the recombinant gene products. Since, however, the cell growth media contain heterologous protein or peptide mixtures like fetal calf serum, the method had to be improved to avoid the adsorption of non human proteins or peptides. We are now able to purify AT III from CHO-cell-superna-tants to more than 95 % purity. The characterization of this AT III-product by double immuno diffusion revealed that it is immunologically totally identical with the authentic material from plasma. AT III antigen content, progressive inhibitor activity and heparin cofactor activity compare very well in the final product; hence, it is totally active compared to AT III from plasma.In polyacrylamidegel electrophoresis most of the material migrated differently to the authentic material showing 9 bands in equal distance to each other, instead four in the At III from plasma. After degradation with sialinidase from both AT III preparations identical cleavage products were obtained migrating predominantly as a single band. Hence, the electrophoretic heterogeneity seems to be due to a different degree of sialinyla-tion of the products.

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Matsumura, Takeko, and Y. Kanematsu. "DO A SCIENCE EXPERIMENT FOR FUTURE SCIENTISTS." In Ampere 2019. Valencia: Universitat Politècnica de València, 2019. http://dx.doi.org/10.4995/ampere2019.2019.9895.

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It has been realized that various chemical reactions are accelerated under irradiation of MW. Such Microwave chemistry is known as time-saving, clear and eco-friendly. MW ovens are world-wide domestic tools for cooking which can serve meals quickly. Regardless of its convenience, few understand the essential mechanism of MW ovens. For better understanding of MW chemistry, authors think it is necessary for to introduce elementary knowledge by holding a 1-day program of experiments by using microwave (MW) ovens.“Science with microwave oven”, 1-day program which we developed and named “Hirameki Tokimeki Science” was supported by Japan Society for the promotion of Science, has been performed over four years.More than 100 students of elementary and junior-high school have joined the program.Here we report the program, response from students.Program of experiments: “1: Dyeing handkerchief with onion peer (*1), 2: Cooking of pizza quickly yeast-leavened, 3: Preparation of shining slime with fluorescein dye synthesized in nonsolvent reaction. 4. Plasma in MW oven (*2), etc.”Students realized how MW accelerated chemical reactions and that dyeing under MW was faster and more fixed compared with the conventional methods. Besides, they could enjoy lunch with pizza and dealing with the slime, both they made. They had a good time with a bit of scientific knowledge. Through 1-day program, we can make science more familiar with students, and it will cause young students to become more interested in science, lead them to future research workers.In addition to the “Hirameki Tokimeki (Inspiration and Spark) Program, we have doneVolunteer activities at Ishinomaki, one of the most damaged cities at the Higashi Nihon Big Earthquake, in 2011.“Science with microwave oven” program surely gives students mysterious interest anddream for Science. That is “Inspire and Spark!” (*1) (*2)

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Krasnoshtanova, Alla, and Elisaveta Borovkova. "OBTAINING NUCLEIC ACID PREPARATIONS AND THEIR HYDROLYSATES FROM BIOMASS OF METHANE-OXIDIZING BACTERIA." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/14.

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"Due to the unfavourable environmental, social and economic situation, the need for the treatment of oncological diseases and diseases associated with impaired activity of the immune system is increasing. A lot of these drugs are made on the basis of nucleic acid components, the industrial production of which is practically non-existent in Russia. Therefore, a task of current interest is to develop the basis of the technology for obtaining components of nucleic acids, which can be widely used in medicine as immunomodulatory, wound-healing, antiviral, and diagnostic medicine, as well as for cancer treatment. Most of the described in literature methods of isolating nucleic acid components from plant, animal and microbial raw materials are based on the use of toxic and expensive organic solvents, that’s why it is impossible to apply these methods outside of laboratory conditions. The most promising source of raw materials for nucleic acids is the biomass of microorganisms (yeast and bacteria) from biomass, since the use of such source makes it possible to quickly obtain a large enough amount of biomass, and, consequently, a larger amount of nucleic acids. This allows obtaining DNA in addition to RNA. RNA and DNA substances can be used to obtain nucleosides and nitrogenous bases, which are also widely used in medicine. The purpose of these studies was to select the conditions for the extraction of RNA and DNA from the biomass of methane-oxidizing bacteria in one technological cycle, as well as to compare the efficiency of alkaline and acid hydrolysis of microbial RNA and DNA. The need for a two-stage extraction of nucleic acids from the biomass of methane-oxidizing bacteria in order to separately extract RNA and DNA was Substantiated. It was ascertained that at the first stage of extraction at a temperature of 90 ° C, pH 9.0 for 90 min, at least 85% of RNA is extracted. After the separation of the extract by centrifugation, the partially denuclearized biomass must be re-processed under the same conditions in order to extract DNA by at least 83%. The modes of concentration of RNA and DNA solutions by ultrafiltration were selected. It was found that in order to achieve effective deposition of nucleic acids at the isoelectric point, the concentration of the RNA solution must be carried out on the UPM-10 membrane at the concentration degree of 7, and the DNA solution on the UPM-100 membrane at the concentration degree 6. The dynamics of decomposition of nucleic-protein complexes in the medium of monoammonium phosphate was investigated. It was shown that the transition of NA into solution by at least 80% is achieved at a monoammonium phosphate concentration of 1.7 M, a temperature of 55 ° C for 90 min. The use of 5-fold washing of oligonucleotide substances with acidified water (pH 2.0) to remove excess mineral impurities was substantiated. А comparative assessment of acid and alkaline hydrolysis of RNA and DNA was carried out in order to obtain derivatives of nucleic acids."

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