Academic literature on the topic 'Zebrafish, light sheet microscopy, heart'

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Journal articles on the topic "Zebrafish, light sheet microscopy, heart"

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Scott, Aaron, Lorena Sueiro Ballesteros, Marston Bradshaw, et al. "In Vivo Characterization of Endogenous Cardiovascular Extracellular Vesicles in Larval and Adult Zebrafish." Arteriosclerosis, Thrombosis, and Vascular Biology 41, no. 9 (2021): 2454–68. http://dx.doi.org/10.1161/atvbaha.121.316539.

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Objective: Extracellular vesicles (EVs) facilitate molecular transport across extracellular space, allowing local and systemic signaling during homeostasis and in disease. Extensive studies have described functional roles for EV populations, including during cardiovascular disease, but the in vivo characterization of endogenously produced EVs is still in its infancy. Because of their genetic tractability and live imaging amenability, zebrafish represent an ideal but under-used model to investigate endogenous EVs. We aimed to establish a transgenic zebrafish model to allow the in vivo identific
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Logan, Savannah L., Christopher Dudley, Ryan P. Baker, Michael J. Taormina, Edouard A. Hay, and Raghuveer Parthasarathy. "Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish." PLOS ONE 13, no. 11 (2018): e0198705. http://dx.doi.org/10.1371/journal.pone.0198705.

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Keller, P. J., A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer. "Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy." Science 322, no. 5904 (2008): 1065–69. http://dx.doi.org/10.1126/science.1162493.

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Madrid-Wolff, Jorge, and Manu Forero-Shelton. "Protocol for the Design and Assembly of a Light Sheet Light Field Microscope." Methods and Protocols 2, no. 3 (2019): 56. http://dx.doi.org/10.3390/mps2030056.

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Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must col
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Holland, Daniel B., Thai V. Truong, Jason A. Junge, and Scott E. Fraser. "Immunoimaging with Light Sheet Microscopy: Microglial Dynamics in the Developing Zebrafish Brain." Biophysical Journal 110, no. 3 (2016): 148a. http://dx.doi.org/10.1016/j.bpj.2015.11.834.

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Qiu, Z., F. Cao, Y. Yang, and L. Sun. "Imaging of ultrasound stimulation on zebrafish neural development with light-sheet microscopy." Brain Stimulation 10, no. 2 (2017): 439. http://dx.doi.org/10.1016/j.brs.2017.01.309.

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Bassi, A., B. Schmid, and J. Huisken. "Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development." Development 142, no. 5 (2015): 1016–20. http://dx.doi.org/10.1242/dev.116970.

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Cook, Sophie R., Cerys Bladen, Johanna Smith, et al. "Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann–Pick type C disease and Smith–Lemli–Opitz syndrome using light sheet microscopy." Histochemistry and Cell Biology 154, no. 5 (2020): 565–78. http://dx.doi.org/10.1007/s00418-020-01925-2.

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AbstractLysosomal storage diseases are the most common cause of neurodegeneration in children. They are characterised at the cellular level by the accumulation of storage material within lysosomes. There are very limited therapeutic options, and the search for novel therapies has been hampered as few good small animal models are available. Here, we describe the use of light sheet microscopy to assess lipid storage in drug and morpholino induced zebrafish models of two diseases of cholesterol homeostasis with lysosomal dysfunction: First, Niemann–Pick type C disease (NPC), caused by mutations i
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Fulton, Timothy, Martin O. Lenz, Leila Muresan, Courtney Lancaster, Elizabeth Horton, and Benjamin Steventon. "Long-term in toto cell tracking using lightsheet microscopy of the zebrafish tailbud." Wellcome Open Research 3 (December 23, 2018): 163. http://dx.doi.org/10.12688/wellcomeopenres.14907.1.

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In toto light-sheet imaging allows the tracking of entire growing tissues with high spatial and temporal resolution for many hours. However, this technology requires a sample to be immobilised to ensure that the tissue of interest remains within the field of view throughout the image acquisition period. We have developed a method of mounting and image capture for long-term light-sheet imaging of a growing zebrafish tailbud from the 18 somite stage through to the end of somitogenesis. By tracking the global movement of the tailbud during image acquisition and feeding this back to the microscope
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Fulton, Timothy, Martin O. Lenz, Leila Muresan, et al. "Long-term in toto cell tracking using lightsheet microscopy of the zebrafish tailbud." Wellcome Open Research 3 (July 15, 2019): 163. http://dx.doi.org/10.12688/wellcomeopenres.14907.2.

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In toto light-sheet imaging allows the tracking of entire growing tissues with high spatial and temporal resolution for many hours. However, this technology requires a sample to be immobilised to ensure that the tissue of interest remains within the field of view throughout the image acquisition period. We have developed a method of mounting and image capture for long-term light-sheet imaging of a growing zebrafish tailbud from the 18 somite stage through to the end of somitogenesis. By tracking the global movement of the tailbud during image acquisition and feeding this back to the microscope
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Dissertations / Theses on the topic "Zebrafish, light sheet microscopy, heart"

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Žičkus, Vytautas. "Blood flow measurement in the zebrafish heart using light sheet microscopy." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/30993/.

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The link between haemodynamics and cardiac tissue mechanics is an active area of research in developmental biology. Nevertheless, previous study of fluid-structure interaction in the developing heart was mostly confined to single projection blood flow measurements or computational fluid dynamics simulations using only the information of the heart wall structure. Hence, techniques capable of direct 3D + time resolved blood flow and heart wall motion are necessary to deepen the understanding of the cardiac function in the developing heart. This work presents an imaging system which combines sele
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Weber, Michael. "All-Optical 4D In Vivo Monitoring And Manipulation Of Zebrafish Cardiac Conduction." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-166647.

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The cardiac conduction system is vital for the initiation and maintenance of the heartbeat. Over the recent years, the zebrafish (Danio rerio) has emerged as a promising model organism to study this specialized system. The embryonic zebrafish heart’s unique accessibility for light microscopy has put it in the focus of many cardiac researchers. However, imaging cardiac conduction in vivo remained a challenge. Typically, hearts had to be removed from the animal to make them accessible for fluorescent dyes and electrophysiology. Furthermore, no technique provided enough spatial and temporal reso
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Taormina, Michael. "Developing Methods Based on Light Sheet Fluorescence Microscopy for Biophysical Investigations of Larval Zebrafish." Thesis, University of Oregon, 2014. http://hdl.handle.net/1794/18342.

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Adapting the tools of optical microscopy to the large-scale dynamic systems encountered in the development of multicellular organisms provides a path toward understanding the physical processes necessary for complex life to form and function. Obtaining quantitatively meaningful results from such systems has been challenging due to difficulty spanning the spatial and temporal scales representative of the whole, while also observing the many individual members from which complex and collective behavior emerges. A three-dimensional imaging technique known as light sheet fluorescence microscopy
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Wolf, Sébastien. "The neural substrate of goal-directed locomotion in zebrafish and whole-brain functional imaging with two-photon light-sheet microscopy." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066468/document.

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La première partie de cette thèse présente une revue historique sur les méthodes d'enregistrements d'activité neuronale, suivie par une étude sur une nouvelle technique d'imagerie pour le poisson zèbre : la microscopie par nappe laser 2 photon. En combinant, les avantages de la microscopie 2 photon et l'imagerie par nappe de lumière, le microscope par nappe laser 2 photon garantie des enregistrements à haute vitesse avec un faible taux de lésions photoniques et permet d'éviter l'une des principales limitations du microscope à nappe laser 1 photon: la perturbation du système visuel. La deuxième
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Berndt, Frederic Carl [Verfasser], Jochen [Gutachter] Guck, Jan [Akademischer Betreuer] Brugués, and Jan [Gutachter] Huisken. "Adaptive light sheet microscopy for the systematic analysis of mitotic spindle scaling in zebrafish / Frederic Carl Berndt ; Gutachter: Jochen Guck, Jan Huisken ; Betreuer: Jan Brugués." Dresden : Technische Universität Dresden, 2019. http://d-nb.info/1226898890/34.

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Ma, Pei. "OPTICAL IMAGING OF EMBRYONIC CARDIAC CONDUCTION." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1464714110.

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Weber, Michael. "All-Optical 4D In Vivo Monitoring And Manipulation Of Zebrafish Cardiac Conduction." Doctoral thesis, 2014. https://tud.qucosa.de/id/qucosa%3A28685.

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The cardiac conduction system is vital for the initiation and maintenance of the heartbeat. Over the recent years, the zebrafish (Danio rerio) has emerged as a promising model organism to study this specialized system. The embryonic zebrafish heart’s unique accessibility for light microscopy has put it in the focus of many cardiac researchers. However, imaging cardiac conduction in vivo remained a challenge. Typically, hearts had to be removed from the animal to make them accessible for fluorescent dyes and electrophysiology. Furthermore, no technique provided enough spatial and temporal reso
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Berndt, Frederic Carl. "Adaptive light sheet microscopy for the systematic analysis of mitotic spindle scaling in zebrafish." 2018. https://tud.qucosa.de/id/qucosa%3A33666.

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Multicellular life is formed by an orchestrated interplay of processes on different scales in space and time. Observing and quantitatively measuring these processes in an intact, living organism requires gentle and adaptive imaging. One example of such a process is the scaling of the mitotic spindle during early development. The spindle segregates the chromosomes during cell division and the spindle length determines the positioning of the chromosomes in the successive daughter cells. Thus, adaptation of spindle size to cell size is crucial for proper functioning. Early development is an exce
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Huang, Yu-Hsuan, and 黄于軒. "Light sheet fluorescence microscopy quantify the concentration-dependent effects of alcohol on structural dynamic of zebrafish AV canal." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/fzsj24.

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碩士<br>國立交通大學<br>影像與生醫光電研究所<br>106<br>This research explores optical quantification of the structural dynamics of zebrafish atrioventricular (AV) canal which is a key component in the formation of heart beating rhythm and vital for prevention of backflow of blood from ventricle to atrium under ventricular contraction. Previous research and clinical studies have indicated congenital or acquired pathogenesis of canal diseases attributed to malfunction or malformation of AV valve in both human and vertebrate animal models. Therefore, the development of a method that measures valvular dynamics of z
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Book chapters on the topic "Zebrafish, light sheet microscopy, heart"

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Kugler, Elisabeth, Timothy Chico, and Paul Armitage. "Image Analysis in Light Sheet Fluorescence Microscopy Images of Transgenic Zebrafish Vascular Development." In Communications in Computer and Information Science. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-95921-4_32.

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Conference papers on the topic "Zebrafish, light sheet microscopy, heart"

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Schlaeppi, Anjalie. "How embryonic hearts pump: insights from multi-scale microscopy in zebrafish." In Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020. Royal Microscopical Society, 2020. http://dx.doi.org/10.22443/rms.lsfm2020.9.

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Mahou, Pierre, Julien Vermot, Emmanuel Beaurepaire, and Willy Supatto. "Multiphoton light-sheet microscopy using wavelength mixing: fast multicolor imaging of the beating Zebrafish heart with low photobleaching." In SPIE BiOS, edited by Ammasi Periasamy, Peter T. C. So, and Karsten König. SPIE, 2015. http://dx.doi.org/10.1117/12.2079176.

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Kugler, Elisabeth. "3D Quantification of Zebrafish Brain Vascular Architecture of Light Sheet Fluorescence Microscopy Datasets." In Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020. Royal Microscopical Society, 2020. http://dx.doi.org/10.22443/rms.lsfm2020.6.

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Kner, Peter, Yang Liu, Savannah Dale, et al. "Imaging a seizure model in zebrafish with structured illumination light sheet microscopy." In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, edited by Thomas G. Brown, Carol J. Cogswell, and Tony Wilson. SPIE, 2018. http://dx.doi.org/10.1117/12.2288326.

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Turrini, L., T. Alterini, C. Müllenbroich, et al. "Functional imaging of zebrafish neuronal activity by Bessel beam light-sheet microscopy." In 18th Italian National Conference on Photonic Technologies (Fotonica 2016). Institution of Engineering and Technology, 2016. http://dx.doi.org/10.1049/cp.2016.0926.

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Sanchez Posada, Juliana. "The heart in 4D: novel tools to quantify ECM dynamics and tissue morphology in cardiac development." In Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020. Royal Microscopical Society, 2020. http://dx.doi.org/10.22443/rms.lsfm2020.26.

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Keller, Philipp J., Annette D. Schmidt, Joachim Wittbrodt, and Ernst H. K. Stelzer. "The Zebrafish Digital Embryo: In Toto Reconstruction of Zebrafish Early Embryonic Development with Digital Scanned Laser Light Sheet Fluorescence Microscopy." In European Conference on Biomedical Optics. OSA, 2009. http://dx.doi.org/10.1364/ecbo.2009.7367_0g.

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Keller, Philipp J., Annette D. Schmidt, Joachim Wittbrodt, and Ernst H. K. Stelzer. "The zebrafish digital embryo: in toto reconstruction of zebrafish early embryonic development with digital scanned laser light sheet fluorescence microscopy." In European Conferences on Biomedical Optics, edited by Paul J. Campagnola, Ernst H. K. Stelzer, and Gert von Bally. SPIE, 2009. http://dx.doi.org/10.1117/12.831496.

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Kner, Peter, Yang Liu, Aqsa Malik, et al. "Imaging neural activity in zebrafish larvae with adaptive optics and structured illumination light sheet microscopy." In Adaptive Optics and Wavefront Control for Biological Systems V, edited by Thomas G. Bifano, Sylvain Gigan, and Na Ji. SPIE, 2019. http://dx.doi.org/10.1117/12.2507048.

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Yang, Zhe, Zhenfei Jiao, Shun Huang, and Ling Fu. "Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of larval zebrafish." In International Conference on Photonics and Imaging in Biology and Medicine. OSA, 2017. http://dx.doi.org/10.1364/pibm.2017.w3a.27.

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