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Journal articles on the topic 'Zebrafish, light sheet microscopy, heart'

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

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1

Scott, Aaron, Lorena Sueiro Ballesteros, Marston Bradshaw, et al. "In Vivo Characterization of Endogenous Cardiovascular Extracellular Vesicles in Larval and Adult Zebrafish." Arteriosclerosis, Thrombosis, and Vascular Biology 41, no. 9 (2021): 2454–68. http://dx.doi.org/10.1161/atvbaha.121.316539.

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Objective: Extracellular vesicles (EVs) facilitate molecular transport across extracellular space, allowing local and systemic signaling during homeostasis and in disease. Extensive studies have described functional roles for EV populations, including during cardiovascular disease, but the in vivo characterization of endogenously produced EVs is still in its infancy. Because of their genetic tractability and live imaging amenability, zebrafish represent an ideal but under-used model to investigate endogenous EVs. We aimed to establish a transgenic zebrafish model to allow the in vivo identific

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Logan, Savannah L., Christopher Dudley, Ryan P. Baker, Michael J. Taormina, Edouard A. Hay, and Raghuveer Parthasarathy. "Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish." PLOS ONE 13, no. 11 (2018): e0198705. http://dx.doi.org/10.1371/journal.pone.0198705.

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Keller, P. J., A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer. "Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy." Science 322, no. 5904 (2008): 1065–69. http://dx.doi.org/10.1126/science.1162493.

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4

Madrid-Wolff, Jorge, and Manu Forero-Shelton. "Protocol for the Design and Assembly of a Light Sheet Light Field Microscope." Methods and Protocols 2, no. 3 (2019): 56. http://dx.doi.org/10.3390/mps2030056.

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Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must col

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Holland, Daniel B., Thai V. Truong, Jason A. Junge, and Scott E. Fraser. "Immunoimaging with Light Sheet Microscopy: Microglial Dynamics in the Developing Zebrafish Brain." Biophysical Journal 110, no. 3 (2016): 148a. http://dx.doi.org/10.1016/j.bpj.2015.11.834.

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6

Qiu, Z., F. Cao, Y. Yang, and L. Sun. "Imaging of ultrasound stimulation on zebrafish neural development with light-sheet microscopy." Brain Stimulation 10, no. 2 (2017): 439. http://dx.doi.org/10.1016/j.brs.2017.01.309.

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Bassi, A., B. Schmid, and J. Huisken. "Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development." Development 142, no. 5 (2015): 1016–20. http://dx.doi.org/10.1242/dev.116970.

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8

Cook, Sophie R., Cerys Bladen, Johanna Smith, et al. "Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann–Pick type C disease and Smith–Lemli–Opitz syndrome using light sheet microscopy." Histochemistry and Cell Biology 154, no. 5 (2020): 565–78. http://dx.doi.org/10.1007/s00418-020-01925-2.

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AbstractLysosomal storage diseases are the most common cause of neurodegeneration in children. They are characterised at the cellular level by the accumulation of storage material within lysosomes. There are very limited therapeutic options, and the search for novel therapies has been hampered as few good small animal models are available. Here, we describe the use of light sheet microscopy to assess lipid storage in drug and morpholino induced zebrafish models of two diseases of cholesterol homeostasis with lysosomal dysfunction: First, Niemann–Pick type C disease (NPC), caused by mutations i

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Fulton, Timothy, Martin O. Lenz, Leila Muresan, Courtney Lancaster, Elizabeth Horton, and Benjamin Steventon. "Long-term in toto cell tracking using lightsheet microscopy of the zebrafish tailbud." Wellcome Open Research 3 (December 23, 2018): 163. http://dx.doi.org/10.12688/wellcomeopenres.14907.1.

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In toto light-sheet imaging allows the tracking of entire growing tissues with high spatial and temporal resolution for many hours. However, this technology requires a sample to be immobilised to ensure that the tissue of interest remains within the field of view throughout the image acquisition period. We have developed a method of mounting and image capture for long-term light-sheet imaging of a growing zebrafish tailbud from the 18 somite stage through to the end of somitogenesis. By tracking the global movement of the tailbud during image acquisition and feeding this back to the microscope

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Fulton, Timothy, Martin O. Lenz, Leila Muresan, et al. "Long-term in toto cell tracking using lightsheet microscopy of the zebrafish tailbud." Wellcome Open Research 3 (July 15, 2019): 163. http://dx.doi.org/10.12688/wellcomeopenres.14907.2.

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In toto light-sheet imaging allows the tracking of entire growing tissues with high spatial and temporal resolution for many hours. However, this technology requires a sample to be immobilised to ensure that the tissue of interest remains within the field of view throughout the image acquisition period. We have developed a method of mounting and image capture for long-term light-sheet imaging of a growing zebrafish tailbud from the 18 somite stage through to the end of somitogenesis. By tracking the global movement of the tailbud during image acquisition and feeding this back to the microscope

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11

Liu, Yang, Savannah Dale, Rebecca Ball, et al. "Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy." Neurophotonics 6, no. 01 (2019): 1. http://dx.doi.org/10.1117/1.nph.6.1.015009.

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12

Yang, Zhe, Li Mei, Fei Xia, Qingming Luo, Ling Fu, and Hui Gong. "Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish." Biomedical Optics Express 6, no. 5 (2015): 1797. http://dx.doi.org/10.1364/boe.6.001797.

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13

Keller, P. J., A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer. "Digital Scanned Laser Light-Sheet Fluorescence Microscopy (DSLM) of Zebrafish and Drosophila Embryonic Development." Cold Spring Harbor Protocols 2011, no. 10 (2011): pdb.prot065839. http://dx.doi.org/10.1101/pdb.prot065839.

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14

Aguet, François, Srigokul Upadhyayula, Raphaël Gaudin, et al. "Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy." Molecular Biology of the Cell 27, no. 22 (2016): 3418–35. http://dx.doi.org/10.1091/mbc.e16-03-0164.

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Membrane remodeling is an essential part of transferring components to and from the cell surface and membrane-bound organelles and for changes in cell shape, which are particularly critical during cell division. Earlier analyses, based on classical optical live-cell imaging and mostly restricted by technical necessity to the attached bottom surface, showed persistent formation of endocytic clathrin pits and vesicles during mitosis. Taking advantage of the resolution, speed, and noninvasive illumination of the newly developed lattice light-sheet fluorescence microscope, we reexamined their asse

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Eum, Juneyong, Jina Kwak, Hee Kim, et al. "3D Visualization of Developmental Toxicity of 2,4,6-Trinitrotoluene in Zebrafish Embryogenesis Using Light-Sheet Microscopy." International Journal of Molecular Sciences 17, no. 11 (2016): 1925. http://dx.doi.org/10.3390/ijms17111925.

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16

Taormina, Mike, Matthew Jemielita, April DeLaurier, and Raghuveer Parthasarathy. "Comparing Photodamage Induced by Confocal Microscopy and Light Sheet Fluorescence Imaging of Zebrafish Skeletal Development." Biophysical Journal 102, no. 3 (2012): 196a. http://dx.doi.org/10.1016/j.bpj.2011.11.1068.

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17

Wang, Zhaoqiang, Yichen Ding, Sandro Satta, Mehrdad Roustaei, Peng Fei, and Tzung K. Hsiai. "A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development." PLOS Computational Biology 17, no. 7 (2021): e1009175. http://dx.doi.org/10.1371/journal.pcbi.1009175.

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Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversin

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18

Bernardello, Matteo, Radoslaw J. Gora, Patrick Van Hage, et al. "Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy." Biomedical Optics Express 12, no. 10 (2021): 6205. http://dx.doi.org/10.1364/boe.435103.

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19

Bernardello, Matteo, Maria Marsal, Emilio J. Gualda, and Pablo Loza-Alvarez. "Light-sheet fluorescence microscopy for the in vivo study of microtubule dynamics in the zebrafish embryo." Biomedical Optics Express 12, no. 10 (2021): 6237. http://dx.doi.org/10.1364/boe.438402.

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20

Kugler, Elisabeth, Karen Plant, Timothy Chico, and Paul Armitage. "Enhancement and Segmentation Workflow for the Developing Zebrafish Vasculature." Journal of Imaging 5, no. 1 (2019): 14. http://dx.doi.org/10.3390/jimaging5010014.

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Zebrafish have become an established in vivo vertebrate model to study cardiovascular development and disease. However, most published studies of the zebrafish vascular architecture rely on subjective visual assessment, rather than objective quantification. In this paper, we used state-of-the-art light sheet fluorescence microscopy to visualize the vasculature in transgenic fluorescent reporter zebrafish. Analysis of image quality, vascular enhancement methods, and segmentation approaches were performed in the framework of the open-source software Fiji to allow dissemination and reproducibilit

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Jemielita, Matthew, Michael J. Taormina, April DeLaurier, Charles B. Kimmel, and Raghuveer Parthasarathy. "Comparing phototoxicity during the development of a zebrafish craniofacial bone using confocal and light sheet fluorescence microscopy techniques." Journal of Biophotonics 6, no. 11-12 (2012): 920–28. http://dx.doi.org/10.1002/jbio.201200144.

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22

d'Amora, Marta, Giuseppe Sancataldo, Francesca Cella Zanacchi, and Alberto Diaspro. "Influence of Nanoparticle Exposure on Nervous System Development in Zebrafish Studied by Means of Light Sheet Fluorescence Microscopy." Biophysical Journal 110, no. 3 (2016): 148a. http://dx.doi.org/10.1016/j.bpj.2015.11.835.

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23

Pudelko, Linda, Steven Edwards, Mirela Balan, et al. "An orthotopic glioblastoma animal model suitable for high-throughput screenings." Neuro-Oncology 20, no. 11 (2018): 1475–84. http://dx.doi.org/10.1093/neuonc/noy071.

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Abstract Background Glioblastoma (GBM) is an aggressive form of brain cancer with poor prognosis. Although murine animal models have given valuable insights into the GBM disease biology, they cannot be used in high-throughput screens to identify and profile novel therapies. The only vertebrate model suitable for large-scale screens, the zebrafish, has proven to faithfully recapitulate biology and pathology of human malignancies, and clinically relevant orthotopic zebrafish models have been developed. However, currently available GBM orthotopic zebrafish models do not support high-throughput dr

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Dyck, Papa K. Van, Natasha Hockaden, Emma C. Nelson, et al. "Cauterization as a Simple Method for Regeneration Studies in the Zebrafish Heart." Journal of Cardiovascular Development and Disease 7, no. 4 (2020): 41. http://dx.doi.org/10.3390/jcdd7040041.

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In the last two decades, the zebrafish has emerged as an important model species for heart regeneration studies. Various approaches to model loss of cardiac myocytes and myocardial infarction in the zebrafish have been devised, and have included resection, genetic ablation, and cryoinjury. However, to date, the response of the zebrafish ventricle to cautery injury has not been reported. Here, we describe a simple and reproducible method using cautery injury via a modified nichrome inoculating needle as a probe to model myocardial infarction in the zebrafish ventricle. Using light and electron

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Hammers, Matthew D., Michael J. Taormina, Matthew M. Cerda, et al. "A Bright Fluorescent Probe for H2S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy." Journal of the American Chemical Society 137, no. 32 (2015): 10216–23. http://dx.doi.org/10.1021/jacs.5b04196.

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26

Schulz, Alina, Jana Brendler, Orest Blaschuk, Kathrin Landgraf, Martin Krueger, and Albert M. Ricken. "Non-pathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish." Journal of Histochemistry & Cytochemistry 67, no. 5 (2019): 361–73. http://dx.doi.org/10.1369/0022155418824083.

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In the heart, unidirectional blood flow depends on proper heart valve function. As, in mammals, regulatory mechanisms of early heart valve and bone development are shown to contribute to adult heart valve pathologies, we used the animal model zebrafish (ZF, Danio rerio) to investigate the microarchitecture and differentiation of cardiac valve interstitial cells in the transition from juvenile (35 days) to end of adult breeding (2.5 years) stages. Of note, light microscopy and immunohistochemistry revealed major differences in ZF heart valve microarchitecture when compared with adult mice. We d

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Icha, Jaroslav, Christiane Kunath, Mauricio Rocha-Martins, and Caren Norden. "Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina." Journal of Cell Biology 215, no. 2 (2016): 259–75. http://dx.doi.org/10.1083/jcb.201604095.

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The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RG

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Truong, Thai V., Vikas Trivedi, Le Trinh, et al. "Live 4D Imaging of the Embryonic Vertebrate Heart with Two-Photon Light Sheet Microscopy and Simultaneous Optical Phase Stamping." Biophysical Journal 106, no. 2 (2014): 435a—436a. http://dx.doi.org/10.1016/j.bpj.2013.11.2453.

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29

Shifatu, Olubusola, Sarah Glasshagel-Chilson, Hannah Nelson, et al. "Heart Development, Coronary Vascularization and Ventricular Maturation in a Giant Danio (Devario malabaricus)." Journal of Developmental Biology 6, no. 3 (2018): 19. http://dx.doi.org/10.3390/jdb6030019.

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Giant danios (genus Devario), like zebrafish, are teleosts belonging to the danioninae subfamily of cyprinids. Adult giant danios are used in a variety of investigations aimed at understanding cellular and physiological processes, including heart regeneration. Despite their importance, little is known about development and growth in giant danios, or their cardiac and coronary vessels development. To address this scarcity of knowledge, we performed a systematic study of a giant danio (Devario malabaricus), focusing on its cardiac development, from the segmentation period to ten months post-fert

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Machikhin, Alexander S., Mikhail V. Volkov, Alexander B. Burlakov, Demid D. Khokhlov, and Andrey V. Potemkin. "Blood Vessel Imaging at Pre-Larval Stages of Zebrafish Embryonic Development." Diagnostics 10, no. 11 (2020): 886. http://dx.doi.org/10.3390/diagnostics10110886.

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The zebrafish (Danio rerio) is an increasingly popular animal model biological system. In cardiovascular research, it has been used to model specific cardiac phenomena as well as to identify novel therapies for human cardiovascular disease. While the zebrafish cardiovascular system functioning is well examined at larval stages, the mechanisms by which vessel activity is initiated remain a subject of intense investigation. In this research, we report on an in vivo stain-free blood vessel imaging technique at pre-larval stages of zebrafish embryonic development. We have developed the algorithm f

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Trivedi, Vikas, Thai V. Truong, Le A. Trinh, Daniel B. Holland, Michael Liebling, and Scott E. Fraser. "Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration." Biomedical Optics Express 6, no. 6 (2015): 2056. http://dx.doi.org/10.1364/boe.6.002056.

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Skjolding, L. M., G. Ašmonaitė, R. I. Jølck, et al. "An assessment of the importance of exposure routes to the uptake and internal localisation of fluorescent nanoparticles in zebrafish (Danio rerio), using light sheet microscopy." Nanotoxicology 11, no. 3 (2017): 351–59. http://dx.doi.org/10.1080/17435390.2017.1306128.

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33

Kondow, Akiko, Kiyoshi Ohnuma, Yasuhiro Kamei, et al. "Light‐sheet microscopy‐based 3D single‐cell tracking reveals a correlation between cell cycle and the start of endoderm cell internalization in early zebrafish development." Development, Growth & Differentiation 62, no. 7-8 (2020): 495–502. http://dx.doi.org/10.1111/dgd.12695.

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Parto, Paria, Mina Tadjalli, S. Reza Ghazi, and Mohammad Ali Salamat. "Distribution and Structure of Purkinje Fibers in the Heart of Ostrich (Struthio camelus) with the Special References on the Ultrastructure." International Journal of Zoology 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/293643.

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Purkinje fibers or Purkinje cardiomyocytes are part of the whole complex of the cardiac conduction system, which is today classified as specific heart muscle tissue responsible for the generation of the heart impulses. From the point of view of their distribution, structure and ultrastructural composition of the cardiac conduction system in the ostrich heart were studied by light and electron microscopy. These cells were distributed in cardiac conducting system including SA node, AV node, His bundle and branches as well as endocardium, pericardium, myocardium around the coronary arteries, mode

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McLeish, Jennifer, Timothy Chico, Harriet Taylor, Carl Tucker, Ken Donaldson, and Simon Brown. "Skin exposure to micro- and nano-particles can cause haemostasis in zebrafish larvae." Thrombosis and Haemostasis 103, no. 04 (2010): 797–807. http://dx.doi.org/10.1160/th09-06-0413.

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SummaryLow mass ambient exposure to airborne particles is associated with atherothrombotic events that may be a consequence of the combustion-derived nanoparticle content. There is concern also over the potential cardiovascular impact of manufactured nanoparticles. To better understand the mechanism by which toxic airborne particles can affect cardiovascular function we utilised zebrafish as a genetically tractable model. Using light and confocal fluorescence video-microscopy, we measured heart-rate and blood flow in the dorsal aorta and caudal artery of zebrafish larvae that had been exposed

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Davidson, L. A., and R. E. Keller. "Neural tube closure in Xenopus laevis involves medial migration, directed protrusive activity, cell intercalation and convergent extension." Development 126, no. 20 (1999): 4547–56. http://dx.doi.org/10.1242/dev.126.20.4547.

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We have characterized the cell movements and prospective cell identities as neural folds fuse during neural tube formation in Xenopus laevis. A newly developed whole-mount, two-color fluorescent RNA in situ hybridization method, visualized with confocal microscopy, shows that the dorsal neural tube gene xpax3 and the neural-crest-specific gene xslug are expressed far lateral to the medial site of neural fold fusion and that expression moves medially after fusion. To determine whether cell movements or dynamic changes in gene expression are responsible, we used low-light videomicroscopy followe

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37

Redder, Esther, Nils Kirschnick, Stefanie Bobe, René Hägerling, Nils Rouven Hansmeier, and Friedemann Kiefer. "Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities." PLOS ONE 16, no. 9 (2021): e0249256. http://dx.doi.org/10.1371/journal.pone.0249256.

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Lymphatic vessels are indispensable for tissue fluid homeostasis, transport of solutes and dietary lipids and immune cell trafficking. In contrast to blood vessels, which are easily visible by their erythrocyte cargo, lymphatic vessels are not readily detected in the tissue context. Their invisibility interferes with the analysis of the three-dimensional lymph vessel structure in large tissue volumes and hampers dynamic intravital studies on lymphatic function and pathofunction. An approach to overcome these limitations are mouse models, which express transgenic fluorescent proteins under the

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Scapin, Giorgia, Jennifer Cillis, Taylor Patch, et al. "Piezo1-Sensitive Biomechanical Pulsation Stimulates Long-Term Hematopoietic Stem Cell Formation." Blood 132, Supplement 1 (2018): 3826. http://dx.doi.org/10.1182/blood-2018-99-110265.

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Abstract The birth and development of hemat\opoietic stem cells (HSCs) remain a mystery. During fetal development, a subset of endothelial cells transitions to become HSCs in the aorta-gonad-mesonephros (AGM) region. Blood flow-mediated shear stress and activation of nitric oxide synthase (NOS) were demonstrated to stimulate the endothelial-to-HSC transition in the AGM. However, we showed that malbec (mlbbw306), a zebrafish mutant for cadherin 5, produces HSCs despite circulation arrest and the inhibition of NOS, suggesting that other biomechanical forces, mechanosensation pathways, or epigene

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La Verde, Giacinto, Maria Paola Bianchi, Giusy Antolino, et al. "Diagnostic Value of Minor Salivary Glands Biopsy in Systemic Amyloidosis." Blood 126, no. 23 (2015): 5381. http://dx.doi.org/10.1182/blood.v126.23.5381.5381.

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Abstract Amyloidosis is a potentially fatal condition characterized by deposition of extracellular protein in an abnormal fibrillary form with a β-sheet structure, named amyloid, in several organs, especially in heart, kidney and liver. The tissue biopsy, stained with Congo red and demonstrating amyloid deposits with apple-green birefringence, is required for diagnosis. The biopsy of visceral organs is characterized by higher sensitivity although it requires invasive procedures bearing higher risk of complications, such as bleeding, hematoma and perforation. Therefore, fine-needle abdominal fa

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Wertheimer, Tobias, Christopher Carl Kloss, Enrico Velardi, et al. "Endothelial Cells Promote Endogenous Thymic Regeneration after Injury Via BMP4 Signaling." Blood 124, no. 21 (2014): 2429. http://dx.doi.org/10.1182/blood.v124.21.2429.2429.

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Abstract Thymopoiesis is a complex process involving crosstalk between developing thymocytes and the non-hematopoietic stromal microenvironment, which includes thymic epithelial cells (TECs), fibroblasts and endothelial cells (ECs). Despite its importance, the thymus is exquisitely sensitive to cellular insults, including cytoreductive chemo- and radiation therapy required for successful hematopoietic stem cell transplantation; therefore identification of thymic repair mechanisms will offer promising therapeutic targets for immune regeneration. Recent studies in tissues such as liver, lung and

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Wertheimer, Tobias, Enrico Velardi, Jennifer Tsai, et al. "Mechanisms Governing Endogenous Thymic Regeneration." Blood 130, Suppl_1 (2017): 66. http://dx.doi.org/10.1182/blood.v130.suppl_1.66.66.

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Abstract Endogenous thymic regeneration is a crucial function that allows for renewal of immune competence following immunodepletion caused by common cancer therapies such as cytoreductive chemotherapy or radiation; however, the mechanisms governing this regeneration remain poorly understood. Moreover, despite this capacity, prolonged T cell deficiency is a major clinical hurdle in recipients of hematopoietic stem cell transplantation (HSCT) and can precipitate high morbidity and mortality from opportunistic infections, and may even facilitate malignant relapse. We have recently described a ce

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Huang, Zengxin, Panchun Gu, Dengfeng Kuang, Ping Mi, and Xizeng Feng. "Dynamic imaging of zebrafish heart with multi‐planar light sheet microscopy." Journal of Biophotonics, January 25, 2021. http://dx.doi.org/10.1002/jbio.202000466.

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Weber, Michael, Nico Scherf, Alexander M. Meyer, Daniela Panáková, Peter Kohl, and Jan Huisken. "Cell-accurate optical mapping across the entire developing heart." eLife 6 (December 29, 2017). http://dx.doi.org/10.7554/elife.28307.

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Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-d

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Messerschmidt, Victoria, Zachary Bailey, Kyung In Baek, et al. "Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart." Journal of Visualized Experiments, no. 138 (August 10, 2018). http://dx.doi.org/10.3791/57763.

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45

Zhang, Bohan, Kristofor E. Pas, Toluwani Ijaseun, Hung Cao, Peng Fei, and Juhyun Lee. "Automatic Segmentation and Cardiac Mechanics Analysis of Evolving Zebrafish Using Deep Learning." Frontiers in Cardiovascular Medicine 8 (June 9, 2021). http://dx.doi.org/10.3389/fcvm.2021.675291.

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Background: In the study of early cardiac development, it is essential to acquire accurate volume changes of the heart chambers. Although advanced imaging techniques, such as light-sheet fluorescent microscopy (LSFM), provide an accurate procedure for analyzing the heart structure, rapid, and robust segmentation is required to reduce laborious time and accurately quantify developmental cardiac mechanics.Methods: The traditional biomedical analysis involving segmentation of the intracardiac volume occurs manually, presenting bottlenecks due to enormous data volume at high axial resolution. Our

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Gudapati, Varun, Yichen Ding, Adam D. Langenbacher, Chih-Chiang Chang, Jau-Nian Chen, and Tzung K. Hsiai. "Abstract 17251: Visualization of Neural Crest Cell Migration to the Dorsal Surface of Developing Zebrafish Myocardium." Circulation 138, Suppl_1 (2018). http://dx.doi.org/10.1161/circ.138.suppl_1.17251.

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Introduction: Neural crest (NC) cells are involved in cardiac development, and NC defects account for over 10% of all congenital heart disease. The migratory and morphogenic patterns of these NC cells are not well understood because conventional optical microscopes struggle to acquire images of live embryos with sufficient temporal and spatial resolution without causing major photo-damage. To address these issues, the lab has designed and built a light-sheet fluorescence microscope (LSFM) that is capable of acquiring dual-channel signals with high spatial and temporal resolution. Hypothesis: W

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Weber, Michael, Michaela Mickoleit, and Jan Huisken. "Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish." Journal of Visualized Experiments, no. 84 (February 27, 2014). http://dx.doi.org/10.3791/51119.

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Icha, Jaroslav, Christopher Schmied, Jaydeep Sidhaye, Pavel Tomancak, Stephan Preibisch, and Caren Norden. "Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development." Journal of Visualized Experiments, no. 110 (April 10, 2016). http://dx.doi.org/10.3791/53966.

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Schlaeppi, Anjalie, Alyssa Graves, Michael Weber, and Jan Huisken. "Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos." Journal of Visualized Experiments, no. 174 (August 13, 2021). http://dx.doi.org/10.3791/62741.

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Greer, Cody J., and Timothy E. Holy. "Fast objective coupled planar illumination microscopy." Nature Communications 10, no. 1 (2019). http://dx.doi.org/10.1038/s41467-019-12340-0.

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Abstract:

Abstract Among optical imaging techniques light sheet fluorescence microscopy is one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. However light sheet microscopes are limited by volume scanning rate and/or camera speed. We present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy, a fast light sheet technique that avoids compromising image quality or photon efficiency. Our fast scan system supports 40 Hz i

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