Academic literature on the topic '"zero-length" dimer'

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Journal articles on the topic ""zero-length" dimer"

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Font, B., and E. Aubert-Foucher. "Detection by chemical cross-linking of bovine brain synapsin I self-association." Biochemical Journal 264, no. 3 (1989): 893–99. http://dx.doi.org/10.1042/bj2640893.

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Synapsin I is believed to play an important role in the regulation of neurotransmitter release, since it is able to bind to synaptic vesicles, to the cytoskeleton and to membrane proteins; in addition, it bundles F-actin and microtubules. These properties, which are controlled by phosphorylation, could be explained if synapsin has different and multiple binding sites or if synapsin I is able to form polymers by self-association. In this study we present experimental evidence that synapsin I at low concentration forms self-associated dimers, as revealed after mild treatments with cross-linking
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O'Brien, Lynn M., Christine F. Huggins, and Philip J. Fay. "Interacting Regions in the A1 and A2 Subunits of Factor VIIIa Identified by Zero-Length Cross-Linking." Blood 90, no. 10 (1997): 3943–50. http://dx.doi.org/10.1182/blood.v90.10.3943.

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Abstract Factor VIIIa is a heterotrimer of A1, A2, and A3-C1-C2 subunits, the activity of which is labile due to a weak affinity interaction of the A2 subunit with the A1/A3-C1-C2 dimer. We have used the zero-length cross-linking reagent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), to localize regions of interaction within the A1 and A2 subunits. Reaction of factor VIIIa with EDC resulted in the formation of a cross-linked product of approximately 90 kD consisting of the A1 and A2 subunits as judged by Western blotting. Alkaline resistance of this product indicated an a
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Perés Wingeyer, Silvia Daniela Amanda, Eleonora Roxana Cunto, Cristina Mabel Nogueras, Jorge Alejandro San Juan, Norberto Gomez, and Gabriela Fernanda De Larrañaga. "Biomarkers in sepsis at time zero: intensive care unit scores, plasma measurements and polymorphisms in Argentina." Journal of Infection in Developing Countries 6, no. 07 (2011): 555–62. http://dx.doi.org/10.3855/jidc.2108.

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Introduction: A patient's response to sepsis is influenced by their genetic background. Our objective was to use plasma markers, such as protein C (PC), D-dimer, Plasminogen Activator Inhibitor-1 (PAI-1) levels, and the PAI-1 rs1799889 4G/5G and Tumor Necrosis Factor-α rs1800629 G/A polymorphisms to improve classical intensive care unit (ICU) scores. Methodology: We studied 380 subjects, 166 with sepsis. We performed coagulation tests: plasma PAI-1 and PC levels were evaluated by chromogenic methods; and D-dimer was evaluated by immunoturbidimetric assay. Polymorphisms were performed using for
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Hatzfeld, M., and K. Weber. "The coiled coil of in vitro assembled keratin filaments is a heterodimer of type I and II keratins: use of site-specific mutagenesis and recombinant protein expression." Journal of Cell Biology 110, no. 4 (1990): 1199–210. http://dx.doi.org/10.1083/jcb.110.4.1199.

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Recombinant DNA technology has been used to analyze the first step in keratin intermediate filament (IF) assembly; i.e., the formation of the double stranded coiled coil. Keratins 8 and 18, lacking cysteine, were subjected to site specific in vitro mutagenesis to change one amino acid in the same relative position of the alpha-helical rod domain of both keratins to a cysteine. The mutations lie at position -36 of the rod in a "d" position of the heptad repeat pattern, and thus air oxidation can introduce a zero-length cystine cross-link. Mutant keratins 8 and 18 purified separately from Escher
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Lapan, Kirsty, and Philip Fay. "Interaction of the A1 Subunit of Factor VIIIa and the Serine Protease Domain of Factor X Identified by Zero-length Cross-linking." Thrombosis and Haemostasis 80, no. 09 (1998): 418–22. http://dx.doi.org/10.1055/s-0037-1615223.

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SummaryWe have previously used a solid phase binding assay to localize a Factor X (FX) interactive site to the acidic C-terminus of the A1 subunit of FVIIIa (Lapan KA, Fay PJ. J Biol Chem 1997; 272: 2082-2088). The complex of FVIII-FX was made covalent following reaction with the zero-length cross-linking reagent 1-ethyl-3-(3-dimethylaminopropyl-)carbodiimide hydrochloride (EDC). Western blotting of the thrombin-cleaved complex showed that the A1 subunit of FVIIIa associated with FX heavy chain. The FX-A1 product was also detected following cross-linking to the A1/A3-C1-C2 dimer, but not the a
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Mareev, V. Yu, Yu L. Begrambekova, and Yu V. Mareev. "How evaluate results of treatment in patients with COVID-19? Symptomatic Hospital and Outpatient Clinical Scale for COVID-19 (SHOCS-COVID)." Kardiologiia 60, no. 11 (2020): 35–41. http://dx.doi.org/10.18087/cardio.2020.11.n1439.

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Aim Development of a novel scale for assessing medical state in patients with new coronavirus infection based on clinical and laboratory disease severity's markers, named SHOKS-COVID scale.Material and Methods Clinical Assessment Scale (SHOKS-COVID) is based on1: clinical parameters (respiratory rate, Body temperature, SpO2 need and type of ventilation support) 2: Inflammation markers (C reactive protein (CRP) and prothrombotic marker (D-dimer)) and 3: percent of lungs injury by CT. This scale was used in several clinical studies in patients with varying severity of the course of the COVID 19.
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Li, Donghai, Hsin-Yao Tang, and David W. Speicher. "A Structural Model of the Erythrocyte Spectrin Heterodimer Initiation Site Determined Using Homology Modeling and Chemical Cross-linking." Journal of Biological Chemistry 283, no. 3 (2007): 1553–62. http://dx.doi.org/10.1074/jbc.m706981200.

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Spectrin assembles into an anti-parallel heterodimeric flexible rod-like molecule through a multistep process initiated by a high affinity interaction between discrete complementary homologous motifs or “repeats” near the actin binding domain. Attempts to determine crystallographic structures of this critical dimer initiation complex have so far been unsuccessful. Therefore, in this study we determined the subunit-subunit docking interface and a plausible medium resolution structure of the heterodimer initiation site using homology modeling coupled with structural refinement based on experimen
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Ortega, I., T. Koenig, R. Sinreich, D. Thomson, and R. Volkamer. "The CU 2-dimensional MAX-DOAS instrument – Part 1: Retrieval of NO<sub>2</sub> in 3 dimensions and azimuth dependent OVOC ratios." Atmospheric Measurement Techniques Discussions 7, no. 11 (2014): 11653–709. http://dx.doi.org/10.5194/amtd-7-11653-2014.

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Abstract. We present an innovative instrument telescope, and describe a retrieval method to probe 3-D distributions of atmospheric trace gases that are relevant to air pollution and tropospheric chemistry. The University of Colorado (CU) two dimensional (2-D) Multi-AXis-Differential Optical Absorption Spectroscopy (CU 2D-MAX-DOAS) instrument measures nitrogen dioxide (NO2), formaldehyde (HCHO), glyoxal (CHOCHO), oxygen dimer (O2-O2, or O4) and water vapor (H2O); also nitrous acid (HONO), bromine monoxide (BrO), iodine monoxide (IO) among other gases can in principle be measured. Information ab
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Ortega, I., T. Koenig, R. Sinreich, D. Thomson, and R. Volkamer. "The CU 2-D-MAX-DOAS instrument – Part 1: Retrieval of 3-D distributions of NO<sub>2</sub> and azimuth-dependent OVOC ratios." Atmospheric Measurement Techniques 8, no. 6 (2015): 2371–95. http://dx.doi.org/10.5194/amt-8-2371-2015.

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Abstract. We present an innovative instrument telescope and describe a retrieval method to probe three-dimensional (3-D) distributions of atmospheric trace gases that are relevant to air pollution and tropospheric chemistry. The University of Colorado (CU) two-dimensional (2-D) multi-axis differential optical absorption spectroscopy (CU 2-D-MAX-DOAS) instrument measures nitrogen dioxide (NO2), formaldehyde (HCHO), glyoxal (CHOCHO), oxygen dimer (O2–O2, or O4), and water vapor (H2O); nitrous acid (HONO), bromine monoxide (BrO), and iodine monoxide (IO) are among other gases that can in principl
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Vottariello, Francesca, Chiara Costanzo, Giovanni Gotte, and Massimo Libonati. "“Zero-Length” Dimers of Ribonuclease A: Further Characterization and No Evidence of Cytotoxicity." Bioconjugate Chemistry 21, no. 4 (2010): 635–45. http://dx.doi.org/10.1021/bc900407v.

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Dissertations / Theses on the topic ""zero-length" dimer"

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VOTTARIELLO, FRANCESCA. "OLIGOMERIZATION OF RNase A:a) A STUDY OF THE INFLUENCE OF SERINE 80 RESIDUE ON THE 3D DOMAIN SWAPPING MECHANISMb) “ZERO-LENGTH” DIMERS OF RNase A AND THEIR CATIONIZATION WITH PEI." Doctoral thesis, 2010. http://hdl.handle.net/11562/344075.

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"Zero-length" dimers of ribonuclease A, a novel type of dimers formed by two RNase A molecules bound to each other through a zero-length amide bond [Simons, B.L. et al. (2007) Proteins 66, 183-195], were analyzed, and tested for their possible in vitro cytotoxic activity. Results: (i) Besides dimers, also trimers and higher oligomers can be identified among the products of the covalently linking reaction. (ii) The "zero-length" dimers prepared by us appear not to be a unique species, as was instead reported by Simons et al. The product is heterogeneous, as shown by the involvement in the amide
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Book chapters on the topic ""zero-length" dimer"

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Mohan, V., and J. B. Anderson. "Quantum Monte Carlo calculations of three-body corrections in the interaction of three helium atoms." In Quantum Monte Carlo. Oxford University PressNew York, NY, 2007. http://dx.doi.org/10.1093/oso/9780195310108.003.0069.

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Abstract For inter nuclear distances of about 4 bohr and greater, the potential energy of three helium atoms is very close to pairwise additive. That is, the total interaction energy is very close to the sum of the interaction energies of the three pairs, each in the absence of a third atom. The energy may then be conveniently expressed as where E1 is an atom energy, LlE2 a pairwise interaction energy, and LlEa is a three-body correction term. For pairwise additivity, LlE3 is zero. The correction LlEa is extremely small and is certainly less than 30 microhartrees (about 10 K) for three He atom
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Conference papers on the topic ""zero-length" dimer"

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Smith, Eric, and Al Ferri. "Shock Isolation in Finite-Length Dimer Chains With Linear, Cubic and Hertzian Spring Interactions." In ASME 2013 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/detc2013-13229.

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A numerical investigation to mitigate the effects of shock in finite 1:1 dimer chains is performed. Dimer chains consist of alternating light and heavy masses. Changing the mass ratio has provided interesting results in previous research. In particular, in the case of Hertzian contacts with zero-preload, certain mass ratios have revealed minimal levels of transmitted force. This paper examines this phenomena from the perspective of utilizing it in practical isolation systems. The zero-preload Hertzian contact case is contrasted with chains connected by linear or cubic springs. Through numerica
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