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Journal articles on the topic '"zero-length" dimer'

Author: Grafiati

Published: 11 March 2023

Last updated: 31 July 2025

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1

Font, B., and E. Aubert-Foucher. "Detection by chemical cross-linking of bovine brain synapsin I self-association." Biochemical Journal 264, no. 3 (1989): 893–99. http://dx.doi.org/10.1042/bj2640893.

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Synapsin I is believed to play an important role in the regulation of neurotransmitter release, since it is able to bind to synaptic vesicles, to the cytoskeleton and to membrane proteins; in addition, it bundles F-actin and microtubules. These properties, which are controlled by phosphorylation, could be explained if synapsin has different and multiple binding sites or if synapsin I is able to form polymers by self-association. In this study we present experimental evidence that synapsin I at low concentration forms self-associated dimers, as revealed after mild treatments with cross-linking

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2

O'Brien, Lynn M., Christine F. Huggins, and Philip J. Fay. "Interacting Regions in the A1 and A2 Subunits of Factor VIIIa Identified by Zero-Length Cross-Linking." Blood 90, no. 10 (1997): 3943–50. http://dx.doi.org/10.1182/blood.v90.10.3943.

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Abstract Factor VIIIa is a heterotrimer of A1, A2, and A3-C1-C2 subunits, the activity of which is labile due to a weak affinity interaction of the A2 subunit with the A1/A3-C1-C2 dimer. We have used the zero-length cross-linking reagent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), to localize regions of interaction within the A1 and A2 subunits. Reaction of factor VIIIa with EDC resulted in the formation of a cross-linked product of approximately 90 kD consisting of the A1 and A2 subunits as judged by Western blotting. Alkaline resistance of this product indicated an a

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3

Perés Wingeyer, Silvia Daniela Amanda, Eleonora Roxana Cunto, Cristina Mabel Nogueras, Jorge Alejandro San Juan, Norberto Gomez, and Gabriela Fernanda De Larrañaga. "Biomarkers in sepsis at time zero: intensive care unit scores, plasma measurements and polymorphisms in Argentina." Journal of Infection in Developing Countries 6, no. 07 (2011): 555–62. http://dx.doi.org/10.3855/jidc.2108.

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Introduction: A patient's response to sepsis is influenced by their genetic background. Our objective was to use plasma markers, such as protein C (PC), D-dimer, Plasminogen Activator Inhibitor-1 (PAI-1) levels, and the PAI-1 rs1799889 4G/5G and Tumor Necrosis Factor-α rs1800629 G/A polymorphisms to improve classical intensive care unit (ICU) scores. Methodology: We studied 380 subjects, 166 with sepsis. We performed coagulation tests: plasma PAI-1 and PC levels were evaluated by chromogenic methods; and D-dimer was evaluated by immunoturbidimetric assay. Polymorphisms were performed using for

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4

Hatzfeld, M., and K. Weber. "The coiled coil of in vitro assembled keratin filaments is a heterodimer of type I and II keratins: use of site-specific mutagenesis and recombinant protein expression." Journal of Cell Biology 110, no. 4 (1990): 1199–210. http://dx.doi.org/10.1083/jcb.110.4.1199.

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Recombinant DNA technology has been used to analyze the first step in keratin intermediate filament (IF) assembly; i.e., the formation of the double stranded coiled coil. Keratins 8 and 18, lacking cysteine, were subjected to site specific in vitro mutagenesis to change one amino acid in the same relative position of the alpha-helical rod domain of both keratins to a cysteine. The mutations lie at position -36 of the rod in a "d" position of the heptad repeat pattern, and thus air oxidation can introduce a zero-length cystine cross-link. Mutant keratins 8 and 18 purified separately from Escher

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5

Lapan, Kirsty, and Philip Fay. "Interaction of the A1 Subunit of Factor VIIIa and the Serine Protease Domain of Factor X Identified by Zero-length Cross-linking." Thrombosis and Haemostasis 80, no. 09 (1998): 418–22. http://dx.doi.org/10.1055/s-0037-1615223.

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SummaryWe have previously used a solid phase binding assay to localize a Factor X (FX) interactive site to the acidic C-terminus of the A1 subunit of FVIIIa (Lapan KA, Fay PJ. J Biol Chem 1997; 272: 2082-2088). The complex of FVIII-FX was made covalent following reaction with the zero-length cross-linking reagent 1-ethyl-3-(3-dimethylaminopropyl-)carbodiimide hydrochloride (EDC). Western blotting of the thrombin-cleaved complex showed that the A1 subunit of FVIIIa associated with FX heavy chain. The FX-A1 product was also detected following cross-linking to the A1/A3-C1-C2 dimer, but not the a

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6

Mareev, V. Yu, Yu L. Begrambekova, and Yu V. Mareev. "How evaluate results of treatment in patients with COVID-19? Symptomatic Hospital and Outpatient Clinical Scale for COVID-19 (SHOCS-COVID)." Kardiologiia 60, no. 11 (2020): 35–41. http://dx.doi.org/10.18087/cardio.2020.11.n1439.

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Aim Development of a novel scale for assessing medical state in patients with new coronavirus infection based on clinical and laboratory disease severity's markers, named SHOKS-COVID scale.Material and Methods Clinical Assessment Scale (SHOKS-COVID) is based on1: clinical parameters (respiratory rate, Body temperature, SpO2 need and type of ventilation support) 2: Inflammation markers (C reactive protein (CRP) and prothrombotic marker (D-dimer)) and 3: percent of lungs injury by CT. This scale was used in several clinical studies in patients with varying severity of the course of the COVID 19.

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7

Li, Donghai, Hsin-Yao Tang, and David W. Speicher. "A Structural Model of the Erythrocyte Spectrin Heterodimer Initiation Site Determined Using Homology Modeling and Chemical Cross-linking." Journal of Biological Chemistry 283, no. 3 (2007): 1553–62. http://dx.doi.org/10.1074/jbc.m706981200.

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Spectrin assembles into an anti-parallel heterodimeric flexible rod-like molecule through a multistep process initiated by a high affinity interaction between discrete complementary homologous motifs or “repeats” near the actin binding domain. Attempts to determine crystallographic structures of this critical dimer initiation complex have so far been unsuccessful. Therefore, in this study we determined the subunit-subunit docking interface and a plausible medium resolution structure of the heterodimer initiation site using homology modeling coupled with structural refinement based on experimen

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8

Ortega, I., T. Koenig, R. Sinreich, D. Thomson, and R. Volkamer. "The CU 2-dimensional MAX-DOAS instrument – Part 1: Retrieval of NO<sub>2</sub> in 3 dimensions and azimuth dependent OVOC ratios." Atmospheric Measurement Techniques Discussions 7, no. 11 (2014): 11653–709. http://dx.doi.org/10.5194/amtd-7-11653-2014.

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Abstract. We present an innovative instrument telescope, and describe a retrieval method to probe 3-D distributions of atmospheric trace gases that are relevant to air pollution and tropospheric chemistry. The University of Colorado (CU) two dimensional (2-D) Multi-AXis-Differential Optical Absorption Spectroscopy (CU 2D-MAX-DOAS) instrument measures nitrogen dioxide (NO2), formaldehyde (HCHO), glyoxal (CHOCHO), oxygen dimer (O2-O2, or O4) and water vapor (H2O); also nitrous acid (HONO), bromine monoxide (BrO), iodine monoxide (IO) among other gases can in principle be measured. Information ab

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9

Ortega, I., T. Koenig, R. Sinreich, D. Thomson, and R. Volkamer. "The CU 2-D-MAX-DOAS instrument – Part 1: Retrieval of 3-D distributions of NO<sub>2</sub> and azimuth-dependent OVOC ratios." Atmospheric Measurement Techniques 8, no. 6 (2015): 2371–95. http://dx.doi.org/10.5194/amt-8-2371-2015.

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Abstract. We present an innovative instrument telescope and describe a retrieval method to probe three-dimensional (3-D) distributions of atmospheric trace gases that are relevant to air pollution and tropospheric chemistry. The University of Colorado (CU) two-dimensional (2-D) multi-axis differential optical absorption spectroscopy (CU 2-D-MAX-DOAS) instrument measures nitrogen dioxide (NO2), formaldehyde (HCHO), glyoxal (CHOCHO), oxygen dimer (O2–O2, or O4), and water vapor (H2O); nitrous acid (HONO), bromine monoxide (BrO), and iodine monoxide (IO) are among other gases that can in principl

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10

Vottariello, Francesca, Chiara Costanzo, Giovanni Gotte, and Massimo Libonati. "“Zero-Length” Dimers of Ribonuclease A: Further Characterization and No Evidence of Cytotoxicity." Bioconjugate Chemistry 21, no. 4 (2010): 635–45. http://dx.doi.org/10.1021/bc900407v.

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11

Traut, Robert R., Debendranath Dey, Dmitry E. Bochkariov, et al. "Location and domain structure of Escherichia coli ribosomal protein L7/L12: site specific cysteine cross-linking and attachment of fluorescent probes." Biochemistry and Cell Biology 73, no. 11-12 (1995): 949–58. http://dx.doi.org/10.1139/o95-102.

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Five different variants of L7/L12 containing single cysteine substitutions, two in the N-terminal (NTD) and three in the C-terminal domain (CTD), were produced, modified with [125I]N-[4-(p-azidosalicylamido)butyl]-3-(2′-pyridyldithio) propionamide ([125I]APDP), a sulfhydryl-specific, heterobifunctional, cleavable photo-cross-linking reagent, and reconstituted into ribosomes. These were irradiated, the total proteins were extracted and reductively cleaved, and the cross-linked proteins were identified. The effect of zero-length disulfide cross-linking on binding and activity was also determined

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12

Martin, Olufunke Y., Deepika S. Darbari, Stefanie Margulies, et al. "Pediatric Sickle Cell Disease and the COVID-19 Pandemic: A Year in Review at Children's National Hospital." Blood 138, Supplement 1 (2021): 3036. http://dx.doi.org/10.1182/blood-2021-149808.

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Abstract Background: Over the past year, COVID-19 was declared a global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) resulting in over 100 million cases and &gt;3 million deaths worldwide according to the World Health Organization (WHO). Morbidity and mortality have been highest in adults, particularly in those with underlying conditions including hypertension, diabetes, and obesity. Children, thus far, have largely remained either asymptomatic or presented with mild symptoms. However, sickle cell disease (SCD) was classified as a risk factor for severe C

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13

Yaghoubi, Hashem, Khosro Khajeh, Saman Hosseinkhani, Bijan Ranjbar, and Hossein Naderi-Manesh. "Application of zero-length cross-linking to form lysozyme, horseradish peroxidase and lysozyme–peroxidase dimers: Activity and stability." International Journal of Biological Macromolecules 41, no. 5 (2007): 624–30. http://dx.doi.org/10.1016/j.ijbiomac.2007.08.004.

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14

Walker, R. A., N. K. Pryer, and E. D. Salmon. "Dilution of individual microtubules observed in real time in vitro: evidence that cap size is small and independent of elongation rate." Journal of Cell Biology 114, no. 1 (1991): 73–81. http://dx.doi.org/10.1083/jcb.114.1.73.

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Although the mechanism of microtubule dynamic instability is thought to involve the hydrolysis of tubulin-bound GTP, the mechanism of GTP hydrolysis and the basis of microtubule stability are controversial. Video microscopy of individual microtubules and dilution protocols were used to examine the size and lifetime of the stabilizing cap. Purified porcine brain tubulin (7-23 microM) was assembled at 37 degrees C onto both ends of isolated sea urchin axoneme fragments in a miniature flow cell to give a 10-fold variation in elongation rate. The tubulin concentration in the region of microtubule

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15

Maniar, Rohan, Kushantha P. K. Withanage, Chandra Shahi, Aaron D. Kaplan, John P. Perdew, and Mark R. Pederson. "Symmetry breaking and self-interaction correction in the chromium atom and dimer." Journal of Chemical Physics 160, no. 14 (2024). http://dx.doi.org/10.1063/5.0180863.

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Density functional approximations to the exchange–correlation energy can often identify strongly correlated systems and estimate their energetics through energy-minimizing symmetry-breaking. In particular, the binding energy curve of the strongly correlated chromium dimer is described qualitatively by the local spin density approximation (LSDA) and almost quantitatively by the Perdew–Burke–Ernzerhof generalized gradient approximation (PBE-GGA), where the symmetry breaking is antiferromagnetic for both. Here, we show that a full Perdew–Zunger self-interaction-correction (SIC) to LSDA seems to g

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16

Gao, ning Ning, huilihan Han та Ting-Yun Shi. "Investigation of Eﬁmov features and universality in ⁸⁷Rb-⁴⁰K mixtures with ﬁnite-range interaction". Physica Scripta, 12 лютого 2025. https://doi.org/10.1088/1402-4896/adb52c.

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Abstract The study of Efimov features and their relationships in 40K-87Rb Mixtures has generated extensive discussion, yet the discrepancy between Efimov universality predictions based on the zero-range approximation and experimental observations remains unresolved. In this study, we investigate the three-body collision properties with J=0 symmetry for a 87Rb-87Rb-40K system on both sides of Rb-K scattering length to understand the mechanisms underlying this discrepancy. Our approach employs the R-matrix propagation method within a hyperspherical coordinate frame, utilizing the Lennard-Jones m

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17

Smith, Eric, and Aldo Ferri. "Shock Isolation in Finite-Length Dimer Chains With Linear, Cubic, and Hertzian Spring Interactions." Journal of Vibration and Acoustics 138, no. 1 (2015). http://dx.doi.org/10.1115/1.4031741.

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This paper investigates the use of finite 1:1 dimer chains to mitigate the transmission of shock disturbances. Dimer chains consist of alternating light and heavy masses with interconnecting compliance. Changing the mass ratio has provided interesting results in previous research. In particular, in the case of Hertzian contacts with zero-preload, certain mass ratios have revealed minimal levels of transmitted force. This paper examines this phenomenon from the perspective of utilizing it in practical isolation systems. The zero-preload Hertzian contact case is contrasted with chains connected

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18

Veríssimo, Luan Martins, Maria Socorro Pereira, Jozef Strecka, and Marcelo L. Lyra. "Ground-state phase diagram and universality of sequential topological valence-bond-solid quantum transitions in a mixed tetramer chain." Journal of Physics: Condensed Matter, January 9, 2024. http://dx.doi.org/10.1088/1361-648x/ad1ca4.

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Abstract The ground-state ordering of a quantum mixed-spin Heisenberg tetramer chain composed of an alternate sequence of $s=1$ and $S=3/2$ dimers is studied in detail as a function of two considered exchange interactions ascribed to similar and dissimilar spin pairs. At zero magnetic field, the ferrimagnetic mixed spin-(1, 1, 3/2, 3/2) Heisenberg tetramer chain displays, depending on a mutual interplay between two considered exchange interactions, three distinct gapped valence-bond-solid phases separated by gap-closing quantum critical points. Using density-matrix renormalization group calcul

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19

Urai, Mizuki, Kazuya Miyagawa, Yuta Watanabe та ін. "Anomalously field-susceptible spin clusters emerging in the electric-dipole liquid candidate κ-(ET) ₂ Hg(SCN) ₂ Br". Science Advances 8, № 51 (2022). http://dx.doi.org/10.1126/sciadv.abn1680.

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Mutual interactions in many-body systems bring about various exotic phases, among which liquid-like states failing to order due to frustration are of keen interest. The organic system with an anisotropic triangular lattice of molecular dimers, κ-(ET) 2 Hg(SCN) 2 Br, has been suggested to host a dipole liquid arising from intradimer charge-imbalance instability, possibly offering an unprecedented stage for the spin degrees of freedom. Here, we show that an extraordinary unordered/unfrozen spin state having soft matter–like spatiotemporal characteristics emerges in this system. 1 H nuclear magne

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