Academic literature on the topic 'Ziehl-Neelsen staining'

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Journal articles on the topic "Ziehl-Neelsen staining"

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Halawa, Felisitas Putri Abadi, Seri Rayani Bangun, and Rica Vera Br Taringan. "Perbandingan Skor Basil Tahan Asam Pewarnaan Ziehl Neelsen dan Ziehl Neelsen Bleach 2% Spesimen Sputum pada Penderita Tuberkulosis Paru di Rumah Sakit Santa Elisabeth Medan." MAHESA : Malahayati Health Student Journal 3, no. 10 (2023): 3225–33. http://dx.doi.org/10.33024/mahesa.v3i10.11170.

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ABSTRACT Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. One way to diagnose this disease is by microscopic examination using the Ziehl Neelsen method. Bleach is a decontaminant solution that can help reduce microscopic dirt and sterilize microscopic fields of view. This study aims to determine the comparison of Ziehl Neelsen staining acid fast bacilli scores and Ziehl Neelsen Bleach 2% sputum specimens in patients with pulmonary tuberculosis. The design used is pre-experimental with 36 sputum samples taken. Data analysis is performed by bivariate analysis usin
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Che, Nanying, Yang Qu, Chen Zhang, Li Zhang, and Haiqing Zhang. "Double staining of bacilli and antigen Ag85B improves the accuracy of the pathological diagnosis of pulmonary tuberculosis." Journal of Clinical Pathology 69, no. 7 (2015): 600–606. http://dx.doi.org/10.1136/jclinpath-2015-203244.

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BackgroundA pathological examination plays an important role in the confirmation of a diagnosis of tuberculosis, especially for smear- and culture-negative cases. However, conventional Ziehl–Neelsen staining and histological tests lack sensitivity and specificity.ObjectiveTo evaluate the diagnostic value of immunohistochemical staining to detect Mycobacterium tuberculosis protein Ag85B and a newly developed double staining (ZC staining) method that can simultaneously detect acid-fast bacilli and M. tuberculosis antigen in the same histological section.MethodsA total of 282 formalin-fixed paraf
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Zahra, Fatima Tuz, Muhammad Zeeshan Saleem, Irfan Ali Mirza, Fatima Sana, Ashfaq Hussain, and Syed Qamar Zaman. "Comparison of Light Emitting Diode (LED) Fluorescent Microscopy with Ziehl-Neelsen Microscopy on Sputum Specimens for Diagnosis of Pulmonary Tuberculosis Keeping Culture as ‘Gold Standard’." Pakistan Armed Forces Medical Journal 72, no. 3 (2022): 763–66. http://dx.doi.org/10.51253/pafmj.v72i3.4323.

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Objective: To compare LED fluorescence microscopy and Ziehl-Neelsen staining in terms of their diagnostic performance in diagnosing pulmonary tuberculosis, taking sputum specimens from patients suspected of pulmonary tuberculosis.
 Study Design: Prospective longitudinal study.
 Place and Duration of Study: Microbiology Department, Armed Forces Institute of Pathology Rawalpindi, from Jan 2019 to Dec 2019.
 Methodology: Sputum samples from patients with clinical suspicion of pulmonary tuberculosis were stained using Ziehl-Neelsen (ZN) stain, fluorescent stain with Auramine O stain
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Slesak, Günther, Saythong Inthalad, Phadsana Basy, et al. "Ziehl-Neelsen Staining Technique Can Diagnose Paragonimiasis." PLoS Neglected Tropical Diseases 5, no. 5 (2011): e1048. http://dx.doi.org/10.1371/journal.pntd.0001048.

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Purbasha, Ghosh, Kar Soumen, Mandal Kaushik, and Nandi Poulami. "A Hospital-Based Comparative Study of Modified Ziehl Neelsen Staining in The Diagnosis of Osteoarticular Tuberculosis Patients." International Journal of Pharmaceutical and Clinical Research 15, no. 6 (2023): 234–38. https://doi.org/10.5281/zenodo.12119605.

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<strong>Objectives:</strong>&nbsp;In this study, the prevalence of osteoarticular tuberculosis (OTB) was assessed and the diagnostic efficacy of modified Ziehl Neelsen staining (MZN) vs standard Ziehl Neelsen staining was compared.&nbsp;<strong>Methods:</strong>&nbsp;In this cross sectional and comparative research, individuals with clinically proven cases of OTB who were older than 18years were included. Exclusion criteria included patients who are not willing to participate or less than 18years of age. As an outpatient procedure, FNAC is done to obtain the clinical sample following all the s
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Bhatt, Chandra Prakash, B. Timalsina, B. Kutu, R. Pradhan, B. Maharjan, and B. Shrestha. "A Comparision of Laboratory Diagnostic Methods of Tuberculosis and Aetiology of Suspected Cases of Pulmonary Tuberculosis." SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS 11, no. 2 (2015): 1–6. http://dx.doi.org/10.3126/saarctb.v11i2.12427.

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Introduction: Tuberculosis remains a worldwide public health problem despite the highly effective drugs and vaccines are available making tuberculosis a preventable and curable disease. The objective of this study was to compare the different laboratory diagnostic methods of tuberculosis and determine its prevalence. Methodology: Morning sputum samples were collected from suspected cases of pulmonary tuberculosis and preceded for Ziehl Neelsen staining, fluorescent staining (auramin-O) and mycobacterium culture in Lowenstein Jensen medium. Results: Total 78 suspected cases of pulmonary tubercu
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Broor, Shobha, Mukesh Sharma, Monika Sharma, Megha Maheshwari, and Dharampal Singh Sudan. "Auramine-O Staining vs Ziehl Neelsen Staining: Advantages and Disadvantages." Indian Journal of Chest Diseases and Allied Sciences 65, no. 1 (2023): 39–43. http://dx.doi.org/10.5005/jp-journals-11007-0062.

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Sinaga, Eka Margaretha, Maniur A. Siahaan, and Apriska Dewi Sipayung. "ANALISA BAKTERI TAHAN ASAM PADA DAHAK PEROKOK AKTIF PENDERITA TUBERKULOSIS PARU." JURNAL ANALIS LABORATORIUM MEDIK 8, no. 1 (2023): 45–49. http://dx.doi.org/10.51544/jalm.v8i1.3834.

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Acid fast bacteria (AFB) are cell walls containing complex lipids so that in Ziehl-Neelsen staining they can bind dyes because they are not washed off by 3% HCl Alcohol (Alcoholic Acid). One of the host factors in pulmonary tuberculosis is smoking habits and exposure. Smoking habits will damage the defense mechanism of the lungs, which causes the lungs to be more easily infected with tuberculosis germs. The purpose of this study was to determine the results of analysis of acid-fast bacteria in the sputum of active smokers. The research method used was descriptive cross-sectional by examining a
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Thakur, Brijesh, Ravi Mehrotra, and Jitendra Singh Nigam. "Correlation of Various Techniques in Diagnosis of Tuberculous Lymphadenitis on Fine Needle Aspiration Cytology." Pathology Research International 2013 (September 19, 2013): 1–4. http://dx.doi.org/10.1155/2013/824620.

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Objective. To study the correlation of cytomorphological features in fine needle aspiration smears from patients suspected of having tuberculous lymphadenitis with Ziehl-Neelsen staining (ZN), auramine-rhodamine staining (ARS), and autofluorescence (AF). Methods. A total of 145 lymph nodes were aspirated, 3 air-dried smears were stained with Giemsa, Ziehl-Neelsen, and auramine-rhodamine stains, and 1 smear was wet fixed for Papanicolaou staining. Needle washes were incubated in Lowenstein-Jensen medium for culture. Papanicolaou and auramine-rhodamine stained smears were examined under fluoresc
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Smith, H. V., A. McDiarmid, A. L. Smith, A. R. Hinson, and R. A. Gilmour. "An analysis of staining methods for the detection ofCryptosporidiumspp. oocysts in water-related samples." Parasitology 99, no. 3 (1989): 323–27. http://dx.doi.org/10.1017/s0031182000059023.

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SummaryThe ability of five staining techniques, originally developed for the rapid identification ofCryptosporidiumspp. oocysts in faecal samples, to detect oocysts in water and water-related samples was assessed. All the stains used (modified Ziehl Neelsen, auramine-phenol (Lempert), Wright-Giemsa, safranin-methylene blue and FITC-labelled monoclonal antibody) stained oocysts after storage in water for 2 months at 4 °C (71–89 % of control values). Storage of oocysts below 0 °C greatly reduced the staining ability of auramine-phenol. With the exception of oocysts stored in raw and final waters
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Dissertations / Theses on the topic "Ziehl-Neelsen staining"

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Rabie, Ursula. "The contribution of the placenta to the diagnosis of congenital tuberculosis." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86623.

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Thesis (MMed)--Stellenbosch University, 2014.<br>ENGLISH ABSTRACT: The aim of this pilot project was to determine whether mothers with laboratory confirmed or clinically suspected tuberculosis (TB) had evidence of TB in the placenta. A secondary objective was to correlate evidence of placental TB with neonatal outcome. A total of 56 placentas were examined to determine if there were any specific histopathological features predictive of tuberculosis together with Ziehl-Neelsen (ZN) staining. A total of 30 cases were positive for maternal TB and one case was a false positive maternal diagnosi
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Book chapters on the topic "Ziehl-Neelsen staining"

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Mehlhorn, Heinz. "Ziehl-Neelsen Staining." In Encyclopedia of Parasitology. Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-43978-4_3449.

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Mehlhorn, Heinz. "Ziehl-Neelsen Staining." In Encyclopedia of Parasitology. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-27769-6_3449-2.

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Harrison, Michael. "Microscopy Skills: Cell Counts, Gram Stains, Ziehl-Neelsen Staining (ZN) and Blood Films." In Revolutionizing Tropical Medicine. John Wiley & Sons, Inc., 2019. http://dx.doi.org/10.1002/9781119282686.ch17.

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SenGupta, Manideepa, and Mallika Sengupta. "Ziehl Neelsen Staining." In Practicals in Microbiology. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12695_7.

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Patwardhan, Neeta, Sharad Bhat, and Satish Patwardhan. "Ziehl-Neelsen Staining." In Handbook of Practical Examination in Microbiology. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12706_8.

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Mithra, K. S. "Principles of Respiratory Diseases - Tuberculosis a Brief Study." In Intelligent Diagnosis of Lung Cancer and Respiratory Diseases. BENTHAM SCIENCE PUBLISHERS, 2022. http://dx.doi.org/10.2174/9789815050509122010004.

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Tuberculosis (TB) is one of the worst lung infections caused by bacteria called Mycobacterium tuberculosis. It is a disease that can be controlled in cases where it is quickly identified and treated. As sputum microscopy is a simple and low-cost approach, most countries use this technique as an initial step in the diagnosis of TB. For this, the patients' morning sputum is collected and submitted to the Ziehl Neelsen staining procedure before the examination. Then a digital microscope is used, where the images of the sputum slides can be recorded for analysis. In this context, numerous research
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Kaewphinit, Thongchai, Somchai Santiwatanakul, and Kosum Chansiri. "The Detection of Tuberculosis by Loop-Mediated Isothermal Amplification (LAMP) Combined with a Lateral Flow Dipstick." In Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering. IGI Global, 2015. http://dx.doi.org/10.4018/978-1-4666-6363-3.ch013.

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Tuberculosis (TB) is an airborne infectious disease caused by the bacterium Mycobacterium Tuberculosis (MTB) and is a persistent problem in developing countries. Present methods for its detection include normal or nested Polymerase Chain Reaction (PCR) followed by electrophoresis, real-time PCR, Ziehl-Neelsen staining, and culture assay. These techniques entail various disadvantages such as high cost, long assay time and use of toxic substances. Novel loop-mediated isothermal amplification (LAMP) permits DNA to be amplified rapidly under constant temperature. The combination of LAMP and chroma
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Conference papers on the topic "Ziehl-Neelsen staining"

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Shah, Wasib. "To determine diagnostic accuracy of gene xpert and sputum Ziehl-Neelsen staining taking sputum culture as gold standard." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa2779.

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Pompermaier, Carolina, Cassio Fernando Paganini, Willian Ely Pin, Mateus Xavier Schenato, and Tales Antunes Franzini. "TUBERCULOUS LYMPHADENITIS: A CASE REPORT." In XXIV Congresso Brasileiro de Mastologia. Mastology, 2022. http://dx.doi.org/10.29289/259453942022v32s1079.

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Tuberculous lymphadenitis is the infection of lymph nodes by Mycobacterium tuberculosis. In the USA, about 8.5% of the cases of tuberculosis (TB) were characterized by lymphadenitis. The peak occurs between 30 and 40 years of age, primarily in women. Extrapulmonary TB is usually diagnosed in immunocompromised patients. The diagnosis is given by positivity in the AFB (Alcohol-Acid Resistant Bacillus) in Ziehl-Neelsen staining by sample collected by fine-needle puncture or lymph node excision. Cyto and histological analysis demonstrate epithelial cells, caseous necrosis, and necrotic cells. Such
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