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Journal articles on the topic 'Ziehl-Neelsen staining'

Author: Grafiati

Published: 3 June 2025

Last updated: 20 July 2025

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1

Halawa, Felisitas Putri Abadi, Seri Rayani Bangun, and Rica Vera Br Taringan. "Perbandingan Skor Basil Tahan Asam Pewarnaan Ziehl Neelsen dan Ziehl Neelsen Bleach 2% Spesimen Sputum pada Penderita Tuberkulosis Paru di Rumah Sakit Santa Elisabeth Medan." MAHESA : Malahayati Health Student Journal 3, no. 10 (2023): 3225–33. http://dx.doi.org/10.33024/mahesa.v3i10.11170.

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ABSTRACT Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. One way to diagnose this disease is by microscopic examination using the Ziehl Neelsen method. Bleach is a decontaminant solution that can help reduce microscopic dirt and sterilize microscopic fields of view. This study aims to determine the comparison of Ziehl Neelsen staining acid fast bacilli scores and Ziehl Neelsen Bleach 2% sputum specimens in patients with pulmonary tuberculosis. The design used is pre-experimental with 36 sputum samples taken. Data analysis is performed by bivariate analysis usin

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Che, Nanying, Yang Qu, Chen Zhang, Li Zhang, and Haiqing Zhang. "Double staining of bacilli and antigen Ag85B improves the accuracy of the pathological diagnosis of pulmonary tuberculosis." Journal of Clinical Pathology 69, no. 7 (2015): 600–606. http://dx.doi.org/10.1136/jclinpath-2015-203244.

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BackgroundA pathological examination plays an important role in the confirmation of a diagnosis of tuberculosis, especially for smear- and culture-negative cases. However, conventional Ziehl–Neelsen staining and histological tests lack sensitivity and specificity.ObjectiveTo evaluate the diagnostic value of immunohistochemical staining to detect Mycobacterium tuberculosis protein Ag85B and a newly developed double staining (ZC staining) method that can simultaneously detect acid-fast bacilli and M. tuberculosis antigen in the same histological section.MethodsA total of 282 formalin-fixed paraf

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Zahra, Fatima Tuz, Muhammad Zeeshan Saleem, Irfan Ali Mirza, Fatima Sana, Ashfaq Hussain, and Syed Qamar Zaman. "Comparison of Light Emitting Diode (LED) Fluorescent Microscopy with Ziehl-Neelsen Microscopy on Sputum Specimens for Diagnosis of Pulmonary Tuberculosis Keeping Culture as ‘Gold Standard’." Pakistan Armed Forces Medical Journal 72, no. 3 (2022): 763–66. http://dx.doi.org/10.51253/pafmj.v72i3.4323.

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Objective: To compare LED fluorescence microscopy and Ziehl-Neelsen staining in terms of their diagnostic performance in diagnosing pulmonary tuberculosis, taking sputum specimens from patients suspected of pulmonary tuberculosis. Study Design: Prospective longitudinal study. Place and Duration of Study: Microbiology Department, Armed Forces Institute of Pathology Rawalpindi, from Jan 2019 to Dec 2019. Methodology: Sputum samples from patients with clinical suspicion of pulmonary tuberculosis were stained using Ziehl-Neelsen (ZN) stain, fluorescent stain with Auramine O stain

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Slesak, Günther, Saythong Inthalad, Phadsana Basy, et al. "Ziehl-Neelsen Staining Technique Can Diagnose Paragonimiasis." PLoS Neglected Tropical Diseases 5, no. 5 (2011): e1048. http://dx.doi.org/10.1371/journal.pntd.0001048.

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Purbasha, Ghosh, Kar Soumen, Mandal Kaushik, and Nandi Poulami. "A Hospital-Based Comparative Study of Modified Ziehl Neelsen Staining in The Diagnosis of Osteoarticular Tuberculosis Patients." International Journal of Pharmaceutical and Clinical Research 15, no. 6 (2023): 234–38. https://doi.org/10.5281/zenodo.12119605.

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<strong>Objectives:</strong> In this study, the prevalence of osteoarticular tuberculosis (OTB) was assessed and the diagnostic efficacy of modified Ziehl Neelsen staining (MZN) vs standard Ziehl Neelsen staining was compared. <strong>Methods:</strong> In this cross sectional and comparative research, individuals with clinically proven cases of OTB who were older than 18years were included. Exclusion criteria included patients who are not willing to participate or less than 18years of age. As an outpatient procedure, FNAC is done to obtain the clinical sample following all the s

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Bhatt, Chandra Prakash, B. Timalsina, B. Kutu, R. Pradhan, B. Maharjan, and B. Shrestha. "A Comparision of Laboratory Diagnostic Methods of Tuberculosis and Aetiology of Suspected Cases of Pulmonary Tuberculosis." SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS 11, no. 2 (2015): 1–6. http://dx.doi.org/10.3126/saarctb.v11i2.12427.

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Introduction: Tuberculosis remains a worldwide public health problem despite the highly effective drugs and vaccines are available making tuberculosis a preventable and curable disease. The objective of this study was to compare the different laboratory diagnostic methods of tuberculosis and determine its prevalence. Methodology: Morning sputum samples were collected from suspected cases of pulmonary tuberculosis and preceded for Ziehl Neelsen staining, fluorescent staining (auramin-O) and mycobacterium culture in Lowenstein Jensen medium. Results: Total 78 suspected cases of pulmonary tubercu

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Broor, Shobha, Mukesh Sharma, Monika Sharma, Megha Maheshwari, and Dharampal Singh Sudan. "Auramine-O Staining vs Ziehl Neelsen Staining: Advantages and Disadvantages." Indian Journal of Chest Diseases and Allied Sciences 65, no. 1 (2023): 39–43. http://dx.doi.org/10.5005/jp-journals-11007-0062.

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8

Sinaga, Eka Margaretha, Maniur A. Siahaan, and Apriska Dewi Sipayung. "ANALISA BAKTERI TAHAN ASAM PADA DAHAK PEROKOK AKTIF PENDERITA TUBERKULOSIS PARU." JURNAL ANALIS LABORATORIUM MEDIK 8, no. 1 (2023): 45–49. http://dx.doi.org/10.51544/jalm.v8i1.3834.

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Acid fast bacteria (AFB) are cell walls containing complex lipids so that in Ziehl-Neelsen staining they can bind dyes because they are not washed off by 3% HCl Alcohol (Alcoholic Acid). One of the host factors in pulmonary tuberculosis is smoking habits and exposure. Smoking habits will damage the defense mechanism of the lungs, which causes the lungs to be more easily infected with tuberculosis germs. The purpose of this study was to determine the results of analysis of acid-fast bacteria in the sputum of active smokers. The research method used was descriptive cross-sectional by examining a

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9

Thakur, Brijesh, Ravi Mehrotra, and Jitendra Singh Nigam. "Correlation of Various Techniques in Diagnosis of Tuberculous Lymphadenitis on Fine Needle Aspiration Cytology." Pathology Research International 2013 (September 19, 2013): 1–4. http://dx.doi.org/10.1155/2013/824620.

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Objective. To study the correlation of cytomorphological features in fine needle aspiration smears from patients suspected of having tuberculous lymphadenitis with Ziehl-Neelsen staining (ZN), auramine-rhodamine staining (ARS), and autofluorescence (AF). Methods. A total of 145 lymph nodes were aspirated, 3 air-dried smears were stained with Giemsa, Ziehl-Neelsen, and auramine-rhodamine stains, and 1 smear was wet fixed for Papanicolaou staining. Needle washes were incubated in Lowenstein-Jensen medium for culture. Papanicolaou and auramine-rhodamine stained smears were examined under fluoresc

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Smith, H. V., A. McDiarmid, A. L. Smith, A. R. Hinson, and R. A. Gilmour. "An analysis of staining methods for the detection ofCryptosporidiumspp. oocysts in water-related samples." Parasitology 99, no. 3 (1989): 323–27. http://dx.doi.org/10.1017/s0031182000059023.

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SummaryThe ability of five staining techniques, originally developed for the rapid identification ofCryptosporidiumspp. oocysts in faecal samples, to detect oocysts in water and water-related samples was assessed. All the stains used (modified Ziehl Neelsen, auramine-phenol (Lempert), Wright-Giemsa, safranin-methylene blue and FITC-labelled monoclonal antibody) stained oocysts after storage in water for 2 months at 4 °C (71–89 % of control values). Storage of oocysts below 0 °C greatly reduced the staining ability of auramine-phenol. With the exception of oocysts stored in raw and final waters

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Anwar, Aan Yulianingsih, Marini Tri Putri Handayani, Erpi Nurdin, Irma Berliana,HI Lewa, and Artati Artati. "Comparison of Tuberculosis Examination Using Ziehl-Neelsen Method and Molecular Rapid Test." Jurnal Kesehatan Manarang 9, no. 3 (2023): 215. http://dx.doi.org/10.33490/jkm.v9i3.848.

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Tuberculosis is an infectious disease of the respiratory tract caused by infection with the bacteria Mycobacterium tuberculosis. Fast and accurate diagnostic methods are very important for diagnosing and controlling pulmonary TB disease in Indonesia. TB examination using the Zn method is very commonly used even though it has lower sensitivity than TCM. The aim of this study was to compare the results of TB examination using BTA staining with the Molecular Rapid Test. This type of research uses analytical observational methods with a cross-sectional approach. The sample was 40 respondents taken

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Shah, Pooja, Sae Pol, Esther Prabhakaran, et al. "Utility of GeneXpert in Diagnosis of Pediatric Tuberculosis and Detection of Rifampicin Resistance." Perspectives in Medical Research 12, no. 02 (2024): 47–51. http://dx.doi.org/10.47799/pimr.1202.09.

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Abstract Background: Tuberculosis (TB) remains a significant public health challenge in developing countries, including India, where children under 15 years account for 12% of all TB cases. The National Tuberculosis Elimination Programme (NTEP) recommends the use of GeneXpert/Cartridge-Based Nucleic Acid Amplification Test (CBNAAT) for TB diagnosis, followed by Line Probe Assay (LPA) for detecting drug resistance. This study aimed to determine the prevalence of TB and rifampicin resistance in pediatric patients and to evaluate the utility of CBNAAT in comparison with LPA and Ziehl-Neelsen stai

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Hasanuddin, Asni, Jurnal Syarif, and Dita Ellyana Artha. "Identification of Mycobacterium Tuberculosis in Active Smokers with Ziehl–Neelsen Staining Method." International Journal of Public Health Excellence (IJPHE) 2, no. 1 (2022): 266–72. http://dx.doi.org/10.55299/ijphe.v2i1.204.

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Tuberculosis is still a health problem for the people of Indonesia, especially active smokers. There are many risk factors that can cause tuberculosis in active smokers including age at starting smoking, number of cigarettes consumed per day, and how long smoking has been done. The purpose of this study was to determine the results of identifying mycobacterium tuberculosis in active smokers with the Ziehl–Neelsen staining acid resistance test method. This type of research is a descriptive-analytic study that aims to determine the results of identifying Mycobacterium tuberculosis in active smok

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Khanna, Vinay, Kriti Tilak, Archi Ghosh, and Chiranjay Mukhopadhyay. "Modified Negative Staining of Heine for Fast and Inexpensive Screening of Cryptosporidium, Cyclospora, and Cystoisospora spp." International Scholarly Research Notices 2014 (October 20, 2014): 1–4. http://dx.doi.org/10.1155/2014/165424.

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Negative staining technique of Heine is an easy, inexpensive, and rapid way of screening for coccidian parasites of the intestinal tract. But its use as a routine technique for screening of Cryptosporidium, Cyclospora, and Cystoisospora is restricted due to its sensitivity being lower than the gold standard method of modified Ziehl-Neelsen staining. This paper emphasises the modification of original Heine staining technique which has been attempted in order to increase the sensitivity and detection of oocysts of Cryptosporidium, Cyclospora, and Cystoisospora. Modified Heine staining technique

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Vasini Rosell, Brenda, Bernardo Lagleyze, Claudia Morsella, et al. "Evidence of in utero infection by Mycobacterium avium subsp. paratuberculosis using Multiple-Locus Variable-number tandem-repeat Analysis:." Brazilian Journal of Veterinary Research and Animal Science 57, no. 1 (2020): e161653. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2020.161653.

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A pregnant heifer with an advanced clinical stage of paratuberculosis was reported in a herd in Argentina. Thus, the animal was euthanized and samples of organs of the cow and its fetus was taken and cultured for bacteriology in specific medium. Tissues were analyzed by histopathology (hematoxylin-eosin and Ziehl-Neelsen staining). Histopathological analysis of the cow’s samples revealed the presence of lesions consistent with paratuberculosis, and Ziehl-Neelsen staining revealed the presence of acid-fast bacilli, whereas the fetal tissues showed absence of lesions but the presence of acid-fas

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Prafulla, Yerraguntla Divya, Sameeranandana Reddy, and Yerraguntla Shashidhar. "Comparision of pap stained smears by using auto fluorescence method with ziehl-neelsen method for detection of acid fast bacilli (AFB)." Panacea Journal of Medical Sciences 13, no. 3 (2023): 676–80. http://dx.doi.org/10.18231/j.pjms.2023.124.

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The Ziehl-Neelsen staining method though it plays an important role in detecting acid fast bacilli (AFB) by direct microscopy its low sensitivity makes to look for the other alternatives. : This study was undertaken to evaluate the utility of auto fluorescence method on Papanicolaou stain (Pap) in comparison tothe traditional Ziehl-Neelsen stain (ZN) method in detection of AFB from lymph node aspirates. Fine needle aspirations (FNAs) were done in 153 patients with clinical suspicion of tubercular lymphadenitis. Smears from aspirate were processed for routine cytology for Hematoxyllin & Eos

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Chopra, Mukul, Shashi Chopra, Silky Mahajan, and Yadwinder Singh. "Comparative evaluation of Ziehl- Neelsen staining and kinyoun’s staining in the diagnosis of clinically suspected cases of tuberculosis." IP International Journal of Medical Microbiology and Tropical Diseases 8, no. 2 (2022): 149–53. http://dx.doi.org/10.18231/j.ijmmtd.2022.031.

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: Tuberculosis commonly affects lungs but can also be extra-pulmonary. The increasing prevalence of extra-pulmonary manifestation of tuberculosis is a cause for concern. Microbiological diagnosis is essence for the effective treatment of mycobacterium tuberculosis. The smear detection tests differentiate infectious from non-infectious cases. Both Ziehl-Neelsen (Z-N) and kinyoun stain (KC) methods are over 100 years old; these are most economical in terms of processing time, needed equipment and personnel. The objective of research was to evaluate the two smear microscopy methods for the detec

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Silas, Goodluck, Nwakulite Arvin, Williams Azibagir, et al. "Evaluating the Prevalence of Mycobacterium tuberculosis among Symptomatic Individuals in Uyakama Community, Ahoada West, Rivers State, Nigeria." South Asian Journal of Research in Microbiology 19, no. 6 (2025): 15–21. https://doi.org/10.9734/sajrm/2025/v19i6438.

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Tuberculosis is caused by a bacterium called Mycobacterium tuberculosis. The bacteria usually attack the lungs; they can also attack other parts of the body. Over the years, the bacteria strain that causes tuberculosis (Mycobacterium tuberculosis) has developed a lot of resistance, mainly as a result of a lack of compliance with treatment. This work aimed to evaluate the prevalence of Mycobacterium tuberculosis among symptomatic individuals in the Uyakama community, Ahoada West, Rivers State, Nigeria. The objectives are to isolate Mycobacterium tuberculosis among male and female subjects and t

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Ahmed, Gehan Mohammed, Ali Seed Ahmed Mohammed, Albadawi Abdulbagi Taha, et al. "Comparison of the Microwave-Heated Ziehl-Neelsen Stain and Conventional Ziehl-Neelsen Method in the Detection of Acid-Fast Bacilli in Lymph Node Biopsies." Open Access Macedonian Journal of Medical Sciences 7, no. 6 (2019): 903–7. http://dx.doi.org/10.3889/oamjms.2019.215.

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BACKGROUND: Tuberculosis is a chronic inflammatory disease with lymphadenopathy being the most common extra-pulmonary manifestation. The conventional Ziehl–Neelsen method plays an essential role in the diagnosis of tuberculosis; however, it has a low sensitivity in detecting acid-fast bacilli. AIM: The present study emphasises the role of the microwave-heated method (modified Ziehl–Neelsen) over conventional Ziehl-Neelsen stain and to set at the best condition for irradiation. MATERIAL AND METHODS: The study included 90 patients with clinically suspected tuberculous lymphadenopathy

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DADDA, A., A. MOHAMED-CHERIF, KH AIT-OUDHIA, L. AOUN, and D. KHELEF. "Epidemiology of cryptosporidiosis in dairy calves in central and eastern Algeria." Journal of the Hellenic Veterinary Medical Society 72, no. 4 (2022): 3285. http://dx.doi.org/10.12681/jhvms.29361.

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The purpose of our survey is to estimate the frequency of Cryptosporidium spp in dairy calves under 60 days of age, the pathogenic role of Cryptosporidium spp in the development of diarrhea, the intervention and association of other enteropathogens often involved in neonatal diarrhea and the possible relationship between infection and certain farming practices in order to estimate the share of risk factors, and on the other hand, to compare between the modified Ziehl-Neelsen staining method and the copro-ELISA test. We found 35 positive samples out of 223 faecal samples analyzed by modified Zi

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Pohjola, S., L. Jokipii, and A. Jokipii. "Dimethylsulphoxide-Ziehl-Neelsen staining technique for detection of cryptosporidial oocysts." Veterinary Record 116, no. 16 (1985): 442–43. http://dx.doi.org/10.1136/vr.116.16.442.

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Widodo, Widodo, Devi Etivia Purlinda, and Ahmad Riadi. "COMPARATIVE EXAMINATION OF CONVENTIONAL DIRECT SPUTUM AND INDIRECT SEDIMENTATION ON CYTOSPIN TUBERCULOSIS PATIENT SPUTUM SAMPLES." Jurnal Riset Kesehatan 11, no. 1 (2022): 60–66. http://dx.doi.org/10.31983/jrk.v11i1.8406.

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Microscopic examination of sputum using the Ziehl-Neelsen stain is the gold standard for Tuberculosis (TB), but it must be performed by experts with special skills. The purpose of this study is to accelerate the determination of the microscopic results using the Ziehl-Neelsen stain. This research is an experimental method in which the test sample is treated and the sputum sample is controlled with up to 25 samples. The method development is very important to increase the sensitivity and specificity of the results of TB examination using microscopes. This observation shows that the indirect cyt

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Kodigehalli Chikkanna Swamy, Ashwini, and Anjan Kumar Patra. "A Study on Comparison between Ziehl Neelsen Staining with Auramine Rhodamine Staining in Diagnosis of Paucibacillary Leprosy." International Journal of Science and Research (IJSR) 10, no. 4 (2021): 177–81. https://doi.org/10.21275/sr21402173642.

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Karabey, Mehmet, Hüseyin Can, Tülay Öncü Öner, et al. "Cryptosporidium spp. during chemotherapy: a cross-sectional study of 94 patients with malignant solid tumor." Annals of Saudi Medicine 41, no. 5 (2021): 293–98. http://dx.doi.org/10.5144/0256-4947.2021.293.

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BACKGROUND: Cryptosporidium spp . is a protozoan parasite that infects many vertebrate animals, including humans. Since Cryptosporidium spp . can cause chronic life-threatening diarrhea and severe malabsorption in immunocompromised patients, we investigated the prevalence of this parasite among patients undergoing chemotherapy for malignant solid tumors. OBJECTIVE: Investigate the prevalence of Cryptosporidium spp . in stool samples. DESIGN: Cross-sectional. SETTING: Tertiary care. PATIENTS AND METHODS: Stool samples were collected from adult patients with malignant solid tumors receiving chem

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Karimi, Shirin, Masoud Shamaei, Mihan Pourabdollah, et al. "Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis." Tuberculosis Research and Treatment 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/858396.

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Purpose. The histological diagnosis of Mycobacterium tuberculosis (MTB) remains a diagnostic challenge despite different methods. Immunohistochemistry (IHC) not only could confirm granulomatous tissue involvement but also can demonstrate MTB antigen immunolocalization. This study tries to clarify the details of immunohistochemical staining for MTB with pAbBCG.Materials/Methods. Twenty-three confirmed TB granulomatous tissue samples were studied by Ziehl-Neelsen and immunohistochemistry (IHC) staining with pAbBCG. Samples were selected from the archive of the Department of Pathology, National R

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Sonhaye, Lantam, Abdoulatif Amadou, Faré Gnandi-Piou, et al. "Tuberculous Abscess of the Chest Wall Simulate Pyogenic Abscess." Case Reports in Radiology 2015 (2015): 1–3. http://dx.doi.org/10.1155/2015/195412.

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The chest wall tuberculosis abscesses is rare. We present a case of a 27-year-old immunocompetent male who presented chest wall abscesses. Imaging (chest radiographic, ultrasound, and computed tomography) and Ziehl-Neelsen staining demonstrated chest wall tuberculosis abscesses.

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Clarke, S. C., and M. McIntyre. "Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis." Journal of Clinical Pathology 49, no. 6 (1996): 511–12. http://dx.doi.org/10.1136/jcp.49.6.511.

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Joseph, A. A., and G. O. Popoola. "Comparison of various staining techniques in the diagnosis of Coccidian parasitosis in HIV infection." Journal of Medical and Biomedical Sciences 5, no. 3 (2017): 28–35. http://dx.doi.org/10.4314/jmbs.v5i3.3.

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Diarrhoea due to oppourtunistic coccidian parasites is a common clinical presentation in HIV infection. Its management differs from that of diarrhoea due to other protozoa, improvement of immune status being the mainstay while specific drug treatment is available for other aetiologies, hence, the need for its accurate identification when present. This can be achieved via various diagnostic techniques, commonly microscopy in this environment, hence the need to compare the efficacy of the commonly used stains in our locality. The objective of the study is to identify the most effective of the co

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KILINÇ, ÖZLEM ORUNÇ, ALI BILGIN YILMAZ, YASAR GÖZ, CUMALI ÖZKAN, and VURAL DENIZHAN. "Determination of Cryptosporidium spp. in Van cats by nested PCR." Medycyna Weterynaryjna 74, no. 1 (2018): 6008–2018. http://dx.doi.org/10.21521/mw.6008.

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The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identif

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A., Ankamma, Rao Kola Kondala, Koyya Gayatri, and Jaya Chandra T. "A Study to Find the Utility of Modified Ziehl Neelsen Staining in the Diagnosis of Pulmonary Tuberculosis among the Pregnant Women." International Journal of Toxicological and Pharmacological Research 12, no. 8 (2022): 366–69. https://doi.org/10.5281/zenodo.11619419.

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<strong>Introduction: </strong>The utility of modified Ziehl Neelsen (MZN) staining is similar to Ziehl Neelsen (ZN) staining in the diagnosis of pulmonary tuberculosis (PT). With this background, a study was conducted to find the diagnostic utility of MZN staining in the pregnant women. <strong>Methods: </strong>It was a prospective study, conducted in the department of Microbiology, GSL Medical College. Pregnant women with diabetes mellitus (DM), symptoms of cough for > 2 weeks were considered. Non cooperative members and non DM were not considered in this research. Initial

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Tilak, K., VK Eshwara, C. Tellapragada, and C. Mukhopadhyay. "Stamp’s modified Ziehl–Neelsen staining for Brucella: Beware of the first impressions." Indian Journal of Medical Microbiology 34, no. 4 (2016): 561–62. http://dx.doi.org/10.4103/0255-0857.195363.

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Gurung, Rajendra, R. Shrestha, N. Poudyal, and SK Bhattacharya. "Ziehl Neelsen vs. Auramine staining technique for detection of acid fast bacilli." Journal of BP Koirala Institute of Health Sciences 1, no. 1 (2018): 59–66. http://dx.doi.org/10.3126/jbpkihs.v1i1.19755.

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Background: The culture and molecular test are the best methods for isolation and identification of Mycobacterium tuberculosis in developed countries. But, in developing countries like Nepal with a significant number of tuberculosis (TB) cases and limited resources, the diagnosis of TB relies primarily on smear microscopy for Acid fast bacilli (AFB).Objective: To compare the results of direct sputum examination for AFB stained by Ziehl Neelsen and Auramine technique.Method: Cross sectional comparative study was conducted in tuberculosis research laboratory, BPKIHS from April to June 2013. A to

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Webster, A., and D. J. Wright. "Ziehl-Neelsen staining of urine deposits in the diagnosis of genitourinary tuberculosis." Journal of Clinical Pathology 38, no. 2 (1985): 236. http://dx.doi.org/10.1136/jcp.38.2.236-a.

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Abdelaziz, Maha M., Wafaa M. K. Bakr, Somya M. Hussien, and Amira E. K. Amine. "Diagnosis of pulmonary tuberculosis using Ziehl–Neelsen stain or cold staining techniques?" Journal of the Egyptian Public Health Association 91, no. 1 (2016): 39–43. http://dx.doi.org/10.1097/01.epx.0000481358.12903.af.

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Danko, M. M., O. L. Tishyn та R. V. Khomyak. "ПОРІВНЯЛЬНА ОЦІНКА КОПРОСКОПІЧНИХ МЕТОДІВ ДІАГНОСТИКИ КРИПТОСПОРИДІОЗУ ВЕЛИКОЇ РОГАТОЇ ХУДОБИ". Scientific Messenger of LNU of Veterinary Medicine and Biotechnology 18, № 3(70) (2016): 71–74. http://dx.doi.org/10.15421/nvlvet7016.

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Cryptosporidiosis in cattle is a significant problem in most countries with intensive livestock, causing significant economic losses. The disease manifests appetite loss, depression, diarrhea, decreased growth, weight loss, retarded growth, and in some cases – death of sick animals. Equally important in the complex treatment and prevention of bovine cryptosporidiosis owned laboratory diagnostics, which allows early disease promptly detect and identify the pathogen. The article presents data on comparative assessment of three coprological methods of laboratory diagnosis of cryptosporidiosis in

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Manrique, Wilson G., Gustavo S. Claudiano, Mayra A. P. Figueiredo, Thalita R. Petrillo, Julieta R. E. Moraes, and Flávio R. de Moraes. "Myxosporidiosis in intensively-reared Piaractus mesopotamicus: Histopathological diagnosis by means of Ziehl-Neelsen staining." Pesquisa Veterinária Brasileira 32, no. 11 (2012): 1133–37. http://dx.doi.org/10.1590/s0100-736x2012001100010.

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Samples of different organs from intensively-reared Piaractus mesopotamicus were collected and processed using routine histological techniques in order to produce thin sections for staining with hematoxylin-eosin and with the Ziehl-Neelsen method. Through examination under an optical microscope, myxosporidians of the genera Henneguya sp. and Myxobolus sp. were identified, respectivelyin the gills and kidneys of P. mesopotamicus. Plasmodia with immature spores of Henneguya sp. were located along the secondary lamellae, with total length of 30.45±4.84µm and width of 3.52±0.33µm. Spores of Myxobo

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Qaiser, Aisha, Muhammad Babar Sohail Niazi, Zaheer Ul Hassan, Shahid Farroq Khattak, Shahid Iqbal, and Sunarays Akbar. "COMPARISON OF VALIDITY OF FNACAND ZIEHL–NEELSEN STAINS IN DIAGNOSING CERVICAL TUBERCULOUS LYMPHADENITIS." PAFMJ 71, Suppl-3 (2021): S484–86. http://dx.doi.org/10.51253/pafmj.v1i1.7928.

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Objective: To compare the validity of FNAC & ZN staining in diagnosing tuberculous lymphadenitis taking mycobacterial culture as gold standard. Study Design: Cross sectional study. Place and Duration of Study: Pathology department, Fauji Foundation Hospital Peshawar, from Oct 2019 to Sep 2020. Methodology: This study includes 100 patients with enlarged cervical lymph nodes who presented as outdoor patients. After routine baseline & radiological investigations, FNAC & ZN staining was carried out, followed by mycobacterial culture. Results: FNAC of tuberculous

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Naureen Saeed, Fatima-tuz-zuhra, Nadeem Ikram, Farhana Shaukat, and Fareeha Sardar. "Comparison of Auramine Phenol and ZN Staining in Diagnosis of Pulmonary Tuberculosis with GeneXpert MTB/RIF as Gold Standard." Journal of Islamabad Medical & Dental College 9, no. 3 (2020): 190–94. http://dx.doi.org/10.35787/jimdc.v9i3.435.

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Background: Pakistan faces an immense burden of pulmonary tuberculosis (TB) due to large number of cases and limited resources. Despite the recent advancement in the diagnostic techniques for pulmonary TB, smear microscopy is still a useful technique for the diagnosis of this disease. This study was conducted in order to compare the diagnostic value of Auramine stain with the conventional Ziehl-Neelsen (ZN) stain on the sputum smear for the diagnosis of pulmonary TB, keeping GeneXpert MTB/RIF as the gold standard. Method: This cross-sectional study was conducted on 356 suspected patients

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Sutriadi, Sutriadi, and Muhammad Taufiq Qurrohman. "UJI KESUAIAN PEMERIKSAAN BTA METODE ZIEHL NEELSEN DENGAN GENEXPERT PADA PASIEN TUBERKULOSIS PARU." Journal of Nursing and Health 9, no. 4 (2024): 465–70. https://doi.org/10.52488/jnh.v9i4.402.

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Tuberculosis is a persistent bacterial infection caused by MycobacteriumTuberculosis. While it primarily affects the lungs, leading to pulmonary TB, the bacteria can also invade other organs, resulting in extrapulmonary TB. Early detection of TB cases is crucial for effective disease management. Combining traditional microscopic methods like Ziehl-Neelsen (ZN) staining with modern molecular technologies such as the GeneXpert molecular rapid test offers significant advantages in TB diagnosis. This approach enhances accuracy and expedites results, facilitating more effective identification and t

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SURYAWATI, BETTY, LELI SAPTAWATI, ASTARI FEBYANE PUTRI, and JATU APHRIDASARI. "Sensitivitas Metode Pemeriksaan Mikroskopis Fluorokrom dan Ziehl-Neelsen untuk Deteksi Mycobacterium tuberculosis pada Sputum." Smart Medical Journal 1, no. 2 (2019): 56. http://dx.doi.org/10.13057/smj.v1i2.28704.

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<p class="AbstractNormal"><em>Background: Detection of fast acid bacteria (FAB) using smear microscopy is used as a primary screening for tuberculosis diagnosis. Previous studies have shown that fluorochrome </em>(<em>Auroamine-rhodamine</em>) <em>staining showed better sensitivity compared to Ziehl-Neelsen (ZN) method in the detection of FAB in sputum. However this method has not been recommended for routine use including in Indonesia. This study aimed to evaluate the sensitivity and specificity of fluorochrome compared to ZN to detect FAB in patient’s sput

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Jain, Suraj Kumar, Kumuda Bandhu Sahoo, and Jaya Chandra T. "Modified Ziehl Neelsen Staining in the Diagnosis of Confirmed Cases of Osteoarticular Tuberculosis." Journal of Evidence Based Medicine and Healthcare 7, no. 41 (2020): 2341–44. http://dx.doi.org/10.18410/jebmh/2020/485.

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Vieira, Soraia, Silvana Belo, and Thomas Hänscheid. "Ziehl–Neelsen in Schistosomiasis: Much More Than Staining the Shell and Species Identification." American Journal of Tropical Medicine and Hygiene 94, no. 4 (2016): 699–700. http://dx.doi.org/10.4269/ajtmh.15-0798.

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MERT, Ali, Resat OZARAS, Muammer BILIR, Fehmi TABAK, Hilal AKI, and Recep OZTURK. "Ziehl-Neelsen staining and polymerase chain reaction study of tissue from tuberculous granulomas." Respirology 8, no. 4 (2003): 548. http://dx.doi.org/10.1046/j.1440-1843.2003.00513.x.

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Hoss, D., A. Berke, M. Murphy, P. Kerr, and J. Grant-Kels. "Acid-Fast (Ziehl-Neelsen) Staining Facilitates Follicular Counting in Horizontally-Sectioned Scalp Biopsies." Journal of Cutaneous Pathology 32, no. 1 (2008): 93. http://dx.doi.org/10.1111/j.0303-6987.2005.320cv.x.

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Ventura-Flores, Roberto. "Diagnóstico de <i>Mycobacterium leprae</i> con Ziehl Neelsen en frotis de piel." Anales de la Facultad de Medicina 85, no. 3 (2024): 378–79. http://dx.doi.org/10.15381/anales.v85i3.28072.

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The diagnosis of leprosy or Hansen's disease is based on a multifaceted approach that includes clinical evidence and laboratory methods that allow the demonstration of Mycobacterium spp to establish the treatment and prognosis of patients. Ziehl Neelsen staining is an alternative to detect the bacillus in skin smears, determine the classification of leprosy based on its bacillary load and control treatment in confirmed patients.

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Indrasari, Witri, Iis Kurniati, Asep Dermawan, and Hafizah Ilmi Sufa. "Confirmation of Mycobacterium tuberculosis culture results with Ziehl-Neelsen staining and MPT64 antigen test." Current Biomedicine 3, no. 1 (2025): 11–15. https://doi.org/10.29244/currbiomed.3.1.11.

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Background Culture of Mycobacterium tuberculosis (MTB) using egg-based solid media like Lowenstein Jensen (LJ) is the gold standard for tuberculosis diagnosis but requires extended incubation time. Rapid diagnostic tests, such as Ziehl-Neelsen (ZN) staining and the MPT64 antigen rapid test, are essential for early detection. Objective This study aimed to evaluate the effectiveness of ZN staining and the MPT64 rapid test in detecting MTB and Mycobacterium other than tuberculosis (MOTT) during different culture times. Methods Using a cross-sectional design, 110 culture-positive samples were anal

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Thapa, Ashok, P. Gurung, and G. R. Ghimire. "Evaluation of Gene Xpert Mtb/Rif Assay for the Detection of Mycobacterium Tuberculosis in Sputum of Patients Suspected of Pulmonary Tuberculosis Visiting National Tuberculosis Centre, Thimi, Bhaktapur, Nepal." SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS 13, no. 1 (2017): 16–22. http://dx.doi.org/10.3126/saarctb.v13i1.16924.

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Introduction: Tuberculosis (TB) is one of the most deadly and common major infectious diseases in developing countries. Rapid and accurate diagnosis of tuberculosis is indispensable to adequately manage the disease and control its transmission. The objective of this study was to evaluate Gene Xpert MTB/RIF Assay for detection of M. tuberculosis in sputum of patients suspected of pulmonary tuberculosis and its comparison with traditional conventional methods.Methodology: A total of 138 patients sputum samples were collected and processed. Gene Xpert MTB/ RIF Assay, culture method and smear micr

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Ahmed, Saqib, Hena A. Ansari, and Nazish Fatima. "A Clinicopathological Study of Fine Needle Aspirates of Lymph Nodes from Patients with Suspected Tubercular Lymphadenopathy: Analysis of 640 Cases from a Tertiary Health Care Centre in North India." International Journal of Health Sciences and Research 12, no. 1 (2022): 201–8. http://dx.doi.org/10.52403/ijhsr.20220127.

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Introduction: Fine needle aspiration cytology (FNAC) of lymph nodes is an out-patient, simple and, quick, cost-effective procedure that helps in the diagnosis of tubercular lymphadenopathy. This technique has high sensitivity and specificity which in addition to the establishment of cytological diagnosis, also helps to perform ancillary tests like ZN staining for AFB Bacilli and GeneXpert MTB/RIF Assay. Aims: To study the clinical and cytopathological features of fine-needle aspirates of lymph nodes from patients with suspected tubercular lymphadenopathy along with the role of ZN staining And

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Velasco, Michele, Marcela Videira, José Ledamir Sindeaux-Neto, et al. "Infection by Henneguya sp. (Myxozoa) in the bone tissue of the gill filaments of the Amazonian catfish Hypophthalmus marginatus (Siluriformes)." Revista Brasileira de Parasitologia Veterinária 24, no. 3 (2015): 365–69. http://dx.doi.org/10.1590/s1984-29612015021.

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This study describes aspects of the infection caused by the myxosporean genus Henneguya, which forms cysts in the bony portion of the gill filaments of Hypophthalmusmarginatus. Specimens of this catfish were acquired dead from artisanal fishermen near the town of Cametá, state of Pará, northern Brazil, between July 2011 and May 2012. They were transported in refrigerated containers to the Carlos Azevedo Research Laboratory at the Federal Rural University of Amazonia, in Belém, where analyses were performed. After confirmation of parasitism by the genus Henneguya, observation were made using op

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Aristoteles, Yuliana Maya Sari, and Denny Juraijin. "DIFFERENCES IN THE RESULTS OF ACID-RESISTANT BACTERIA BY THE ZIEHL NEELLSEN METHOD AND THE RAPID MOLECULAR TEST (TCM) WITH A STORAGE LENGTH OF 48 HOURS." Journal Health Applied Science and Technology 2, no. 1 (2024): 14–19. http://dx.doi.org/10.52523/jhast.v2i1.33.

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Background: Tuberculosis (TBC) is an infectious disease caused by Mycobacterium tuberculosis, a dorect human-to-human infectious disease not caused by witchcraft or curses TB germ most often attack the lungs but can also attack other organs such as the lymph nodes, bones, brain, skin and others. The diagnosis of pulmonary TB is established based on the clinical picture, physical examination, radiological picture, laboratory examination and tuberculin test. The diagnosis of pulmonary tuberculosis currently used routinely in labpratories including hospitals and health centers is a bacteriologica

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