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Journal articles on the topic "Zif268"
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Penke, Zsuzsa, Elise Morice, Alexandra Veyrac, Alexandra Gros, Carine Chagneau, Pascale LeBlanc, Nathalie Samson, et al. "Zif268 / Egr1 gain of function facilitates hippocampal synaptic plasticity and long-term spatial recognition memory." Philosophical Transactions of the Royal Society B: Biological Sciences 369, no. 1633 (January 5, 2014): 20130159. http://dx.doi.org/10.1098/rstb.2013.0159.
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It is well established that Zif268/Egr1 , a member of the Egr family of transcription factors, is critical for the consolidation of several forms of memory; however, it is as yet uncertain whether increasing expression of Zif268 in neurons can facilitate memory formation. Here, we used an inducible transgenic mouse model to specifically induce Zif268 overexpression in forebrain neurons and examined the effect on recognition memory and hippocampal synaptic transmission and plasticity. We found that Zif268 overexpression during the establishment of memory for objects did not change the ability to form a long-term memory of objects, but enhanced the capacity to form a long-term memory of the spatial location of objects. This enhancement was paralleled by increased long-term potentiation in the dentate gyrus of the hippocampus and by increased activity-dependent expression of Zif268 and selected Zif268 target genes. These results provide novel evidence that transcriptional mechanisms engaging Zif268 contribute to determining the strength of newly encoded memories.
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Christy, B., and D. Nathans. "Functional serum response elements upstream of the growth factor-inducible gene zif268." Molecular and Cellular Biology 9, no. 11 (November 1989): 4889–95. http://dx.doi.org/10.1128/mcb.9.11.4889.
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The zif268 gene, which encodes a protein with three typical zinc finger sequences, is induced in mouse 3T3 cells by serum, phorbol 12-myristate 13-acetate platelet-derived growth factor, and fibroblast growth factor. The induction is coordinate with that of c-fos. The 5'-flanking region of zif268 contains sequences that resemble known regulatory elements, including four CC(A or T)6GG sequences similar to the core serum response elements (SREs) found upstream of c-fos and actin genes. To determine whether the zif268 SRE-like elements mediate induction, CAT (chloramphenicol acetyltransferase) plasmids with different lengths of zif268 upstream sequences were tested for inducibility in 3T3 cells by serum, platelet-derived growth factor, or phorbol 12-myristate 13-acetate. In addition, double-stranded oligonucleotides corresponding to each of the four zif268 putative SREs were tested individually for responsiveness when placed upstream of a thymidine kinase gene promoter. Each of the four SREs conferred inducibility by the agents tested, and multiple SREs resulted in greater inducibility than did a single element. Each of the zif268 SREs also competed with the c-fos SRE for binding by serum response factor present in HeLa cell nuclear extract. We conclude that the zif268 SRE-like sequences are functional and probably account for the coordinate induction of zif268 and c-fos.
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Christy, B., and D. Nathans. "Functional serum response elements upstream of the growth factor-inducible gene zif268." Molecular and Cellular Biology 9, no. 11 (November 1989): 4889–95. http://dx.doi.org/10.1128/mcb.9.11.4889-4895.1989.
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The zif268 gene, which encodes a protein with three typical zinc finger sequences, is induced in mouse 3T3 cells by serum, phorbol 12-myristate 13-acetate platelet-derived growth factor, and fibroblast growth factor. The induction is coordinate with that of c-fos. The 5'-flanking region of zif268 contains sequences that resemble known regulatory elements, including four CC(A or T)6GG sequences similar to the core serum response elements (SREs) found upstream of c-fos and actin genes. To determine whether the zif268 SRE-like elements mediate induction, CAT (chloramphenicol acetyltransferase) plasmids with different lengths of zif268 upstream sequences were tested for inducibility in 3T3 cells by serum, platelet-derived growth factor, or phorbol 12-myristate 13-acetate. In addition, double-stranded oligonucleotides corresponding to each of the four zif268 putative SREs were tested individually for responsiveness when placed upstream of a thymidine kinase gene promoter. Each of the four SREs conferred inducibility by the agents tested, and multiple SREs resulted in greater inducibility than did a single element. Each of the zif268 SREs also competed with the c-fos SRE for binding by serum response factor present in HeLa cell nuclear extract. We conclude that the zif268 SRE-like sequences are functional and probably account for the coordinate induction of zif268 and c-fos.
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McDade, Donna M., Ann-Marie Conway, Allan B. James, and Brian J. Morris. "Activity-dependent gene transcription as a long-term influence on receptor signalling." Biochemical Society Transactions 37, no. 6 (November 19, 2009): 1375–77. http://dx.doi.org/10.1042/bst0371375.
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The regulation of synaptic glutamate receptor and GABAAR (γ-aminobutyric acid subtype A receptor) levels is a key component of synaptic plasticity. Most forms of neuronal plasticity are associated with the induction of the transcription factor zif268 (egr1). Hence, it is predicted that zif268 may regulate transcription of genes associated with glutamate receptors and/or GABAARs. It turns out that receptor regulation by zif268 tends to be indirect. Induction of zif268 in neurons leads to altered expression of proteasome subunit and proteasome-regulatory genes, thereby changing the capacity of the neuron to degrade synaptic proteins, including receptors and receptor subunits. In addition, zif268 alters the transcription of genes associated with GABAAR expression and trafficking, such as ubiquilin and gephyrin. This indirect regulation of receptor turnover is likely to contribute to the delayed, but long-lasting, phases of synaptic plasticity and also to the synaptic dysfunction associated with diseases such as schizophrenia and Alzheimer's disease, where zif268 expression is reduced.
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Chaudhuri, Avi, Joanne A. Matsubara, and Max S. Cynader. "Neuronal activity in primate visual cortex assessed by immunostaining for the transcription factor Zif268." Visual Neuroscience 12, no. 1 (January 1995): 35–50. http://dx.doi.org/10.1017/s095252380000729x.
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AbstractIt is now well established that environmental signals mediated via neurotransmitters and hormones can induce responses in cells which involve a cascade of receptors, G proteins, and second messengers. These in turn can induce transcription factors which regulate long-term changes in gene expression. It has been proposed that the stimulus-transcription coupling properties of these DNA-binding proteins can be exploited to visualize activated neurons by way of immunostaining. We have used standard immunohistochemical techniques to detect the expression of one specific transcription factor, Zif268, in the visual cortex (area 17, V1) of vervet monkeys (Cercopithecus aethiops). Immunopositive neurons were present in large numbers throughout the visual cortex of the normal animal, being concentrated in layers 2/3 and 6 and at moderate levels in 4Cβ and 5. To determine if Zif268 expression was affected by visual stimulation in the monkey, we restricted light input to one eye with the aim of revealing ocular-dominance columns in striate cortex. We found that short-term monocular deprivation induced either by enucleation, intravitreal TTX injection, or eyelid suturing resulted in dramatic changes in Zif268 levels, revealing vertically oriented columns of reduced Zif268 staining interdigitated with columns of normal expression. Furthermore, these columns were discernible after just 2 h of monocular blockade. A comparison of the ocular-dominance pattern obtained with Zif268 immunostaining and cytochrome oxidase histochemistry in long-term monocularly deprived animals showed a coincident reduction of both markers along columns that were precisely aligned in adjacent sections, indicating that Zif268 expression is restricted to cortical regions of high metabolic activity. Simultaneous immunostaining for Zif268 and the calcium-binding proteins calbindin and parvalbumin showed a negative correlation, suggesting that the Zif268 protein may be expressed selectively within excitatory neurons. A similar approach with immunostaining for neurofilament and microtubule-associated proteins (SMI-32 and MAP2) revealed pyramidal neurons which were regularly found to contain a Zif268-positive nucleus. Furthermore, confocal images of lucifer yellow filled neurons possessing Zif268-positive nuclei all showed pyramidal morphology. Taken together, these results point to activity-dependent expression of Zif268 within a subset of excitatory neurons.
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Suva, L. J., M. Ernst, and G. A. Rodan. "Retinoic acid increases zif268 early gene expression in rat preosteoblastic cells." Molecular and Cellular Biology 11, no. 5 (May 1991): 2503–10. http://dx.doi.org/10.1128/mcb.11.5.2503.
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In this study we demonstrate that retinoic acid (RA) increases the expression of transcription factor zif268 mRNA in primary cultures of fetal rat calvarial cells and in simian virus 40-immortalized clonal rat calvarial preosteoblastic cells (RCT-1), which differentiate in response to RA, but not in the more differentiated RCT-3 and ROS 17/2.8 cells. The increased expression of zif268 mRNA is rapid (maximal within 1 h), transient (returns to basal levels by 3 h), detectable at RA doses of 10(-12)M, and independent of protein synthesis. The relative stimulation of zif268 mRNA by RA was much larger than that of other early genes, including c-fos, c-jun, and junB. The rate of transcription of RA-stimulated RCT-1 cells, estimated by nuclear run-on assays, was elevated, suggesting that RA regulation of zif268 gene transcription was at least in part transcriptional. Moreover, RA stimulated the transcriptional activity of a Zif268CAT (chloramphenicol acetyltransferase) plasmid containing 632 bp of zif268 5' regulatory sequences in RCT-1 cells but not in the more differentiated RCT-3 cells. These in vitro data support the in vivo observations which localize zif268 and RA receptor-gamma transcripts to bone and cartilage during development, suggesting that both RA and zif268 may play a role in osteoblast differentiation.
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Suva, L. J., M. Ernst, and G. A. Rodan. "Retinoic acid increases zif268 early gene expression in rat preosteoblastic cells." Molecular and Cellular Biology 11, no. 5 (May 1991): 2503–10. http://dx.doi.org/10.1128/mcb.11.5.2503-2510.1991.
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In this study we demonstrate that retinoic acid (RA) increases the expression of transcription factor zif268 mRNA in primary cultures of fetal rat calvarial cells and in simian virus 40-immortalized clonal rat calvarial preosteoblastic cells (RCT-1), which differentiate in response to RA, but not in the more differentiated RCT-3 and ROS 17/2.8 cells. The increased expression of zif268 mRNA is rapid (maximal within 1 h), transient (returns to basal levels by 3 h), detectable at RA doses of 10(-12)M, and independent of protein synthesis. The relative stimulation of zif268 mRNA by RA was much larger than that of other early genes, including c-fos, c-jun, and junB. The rate of transcription of RA-stimulated RCT-1 cells, estimated by nuclear run-on assays, was elevated, suggesting that RA regulation of zif268 gene transcription was at least in part transcriptional. Moreover, RA stimulated the transcriptional activity of a Zif268CAT (chloramphenicol acetyltransferase) plasmid containing 632 bp of zif268 5' regulatory sequences in RCT-1 cells but not in the more differentiated RCT-3 cells. These in vitro data support the in vivo observations which localize zif268 and RA receptor-gamma transcripts to bone and cartilage during development, suggesting that both RA and zif268 may play a role in osteoblast differentiation.
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Lonergan, Mary E., Georgette M. Gafford, Timothy J. Jarome, and Fred J. Helmstetter. "Time-Dependent Expression of Arc and Zif268 after Acquisition of Fear Conditioning." Neural Plasticity 2010 (2010): 1–12. http://dx.doi.org/10.1155/2010/139891.
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Memory consolidation requires transcription and translation of new protein. Arc, an effector immediate early gene, and zif268, a regulatory transcription factor, have been implicated in synaptic plasticity underlying learning and memory. This study explored the temporal expression profiles of these proteins in the rat hippocampus following fear conditioning. We observed a time-dependent increase of Arc protein in the dorsal hippocampus 30-to-90-minute post training, returning to basal levels at 4 h. Zif268 protein levels, however, gradually increased at 30-minute post training before peaking in expression at 60 minute. The timing of hippocampal Arc and zif268 expression coincides with the critical period for protein synthesis-dependent memory consolidation following fear conditioning. However, the expression of Arc protein appears to be driven by context exploration, whereas, zif268 expression may be more specifically related to associative learning. These findings suggest that altered Arc and zif268 expression are related to neural plasticity during the formation of fear memory.
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Waye, Shannon C., O. Chandani Dinesh, SM Nageeb Hasan, Joshua D. Conway, Roger Raymond, José N. Nobrega, Jacqueline Blundell, and Francis Rodriguez Bambico. "Antidepressant action of transcranial direct current stimulation in olfactory bulbectomised adolescent rats." Journal of Psychopharmacology 35, no. 8 (April 28, 2021): 1003–16. http://dx.doi.org/10.1177/02698811211000765.
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Background: Antidepressant drugs in adolescent depression are sometimes mired by efficacy issues and paradoxical effects. Transcranial direct current stimulation (tDCS) could represent an alternative. Aims/methods: We tested the antidepressant action of prefrontal tDCS and paroxetine (20 mg/kg, intraperitoneal) in olfactory bulbectomised (OBX) adolescent rats. Using enzyme-linked immunosorbent assays and in situ hybridisation, we examined treatment-induced changes in plasma brain-derived neurotrophic factor (BDNF) and brain serotonin transporter (SERT) and 5-HT-1A mRNA. Results: OBX-induced anhedonia-like reductions in sucrose preference (SP) correlated with open field (OF) hyperactivity. These were accompanied by decreased zif268 mRNA in the piriform/amygdalopiriform transition area, and increased zif268 mRNA in the hypothalamus. Acute paroxetine (2 days) led to a profound SP reduction, an effect blocked by combined tDCS–paroxetine administration. Chronic (14 days) tDCS attenuated hyperlocomotion and its combination with paroxetine blocked OBX-induced SP reduction. Correlations among BDNF, SP and hyperlocomotion scores were altered by OBX but were normalised by tDCS–paroxetine co-treatment. In the brain, paroxetine increased zif268 mRNA in the hippocampal CA1 subregion and decreased it in the claustrum. This effect was blocked by tDCS co-administration, which also increased zif268 in CA2. tDCS-paroxetine co-treatment had variable effects on 5-HT1A receptors and SERT mRNA. 5-HT1A receptor changes were found exclusively within depression-related parahippocampal/hippocampal subregions, and SERT changes within fear/defensive response-modulating brainstem circuits. Conclusion: These findings point towards potential synergistic efficacies of tDCS and paroxetine in the OBX model of adolescent depression via mechanisms associated with altered expression of BDNF, 5-HT1A, SERT and zif268 in discrete corticolimbic areas.
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Powell, Anna L., Emma Hindley, Andrew JD Nelson, Moira Davies, Eman Amin, John P. Aggleton, and Seralynne D. Vann. "Lesions of retrosplenial cortex spare immediate-early gene activity in related limbic regions in the rat." Brain and Neuroscience Advances 2 (January 2018): 239821281881123. http://dx.doi.org/10.1177/2398212818811235.
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The retrosplenial cortex forms part of a network of cortical and subcortical structures that have particular importance for spatial learning and navigation in rodents. This study examined how retrosplenial lesions affect activity in this network by visualising the expression of the immediate-early genes c- fos and zif268 after exposure to a novel location. Groups of rats with extensive cytotoxic lesions (areas 29 and 30) and rats with lesions largely confined to area 30 (dysgranular cortex) were compared with their respective control animals for levels of c- fos expression measured by immunohistochemistry. These cortical lesions had very limited effects on distal c- fos activity. Evidence of a restricted reduction in c-fos activity was seen in the septal dentate gyrus (superior blade) but not in other hippocampal and parahippocampal subareas, nor in the anterior cingulate and prelimbic cortices. Related studies examined zif268 activity in those cases with combined area 29 and 30 lesions. The only clear evidence for reduced zif268 activity following retrosplenial cell loss came from the septal CA3 area. The confined impact of retrosplenial tissue loss is notable as, by the same immediate-early gene measures, retrosplenial cortex is itself highly sensitive to damage in related limbic areas, showing a marked c- fos and zif268 hypoactivity across all of its subareas. This asymmetry in covert pathology may help to explain the apparent disparity between the severity of learning deficits after retrosplenial cortex lesions and after lesions in either the hippocampus or the anterior thalamic nuclei.
Dissertations / Theses on the topic "Zif268"
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Green, Andrew F. D. "Metal ligation in ZIF268, a zinc finger protein, effects on DNA binding." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ45850.pdf.
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McDade, Donna Marie. "Identification of novel target genes for the plasticity-related transcription factor Zif268." Thesis, University of Glasgow, 2007. http://theses.gla.ac.uk/28/.
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Activity based alterations in synaptic connectivity are thought to underlie the processes involved in learning and memory. Measurable changes in neuronal activation by long-term potentiation (LTP) are widely investigated as a possible cellular correlate of this phenomenon, as it can be induced quickly to elicit long-lasting modifications. These long-term changes in the activity of neuronal circuits are sustained by an altered pattern of gene expression and protein synthesis. The inducible transcription factor Zif268 has been implicated in almost all models of neuronal plasticity. Downstream targets of zif268 are widely believed to contribute to the duration and stabilisation of NMDA receptor dependent LTP which, in turn, can be linked to various models of learning and memory. However, these downstream targets are only just starting to receive attention. By utilising a wide range of contemporary neuroscience techniques covering molecular & cell biology approaches, this thesis proposes two known proteins, gephyrin and ubiquilin, as well as a novel gene (urma), as potential downstream targets of Zif268. Both gephyrin and ubiquilin are associated with GABAA receptors at inhibitory synapses. Gephyrin is thought to cluster and anchor GABAA receptors at postsynaptic sites whilst ubiquilin is reported to regulate receptor surface expression. We found that gephyrin mRNA and protein expression levels were downregulated in response to increased levels of zif268 by transient transfection in PC-12 cells and NMDA stimulation in primary cultured cortical neurones. In addition, ubiquilin mRNA and protein levels were also downregulated within the same experimental paradigms, implying that both gephyrin and ubiquilin are downstream transcriptional targets of this plasticity-related gene. A previously reported microarray experiment (James et al. 2005) contained 144 ESTs significantly affected by the transient transfection of Zif268 in PC-12 cells compared to control. Bioinformatic analyses of these tags revealed interesting genomic areas pertaining to little published information. After further investigation, EST AI169020 revealed a novel transcript that was downregulated in response to NMDA treatment of primary cortical neurones. Additional data mining suggests that urma may be a rarely expressed transcription factor. Basal levels of urma mRNA were also decreased in the Zif268 knockout mouse, as were ubiquilin mRNA levels. Zif268 is a regulatory immediate early gene, activating or suppressing downstream targets that play a role in the duration and stabilisation of LTP. These results indicate that gephyrin and ubiquilin are potential mediators of NMDA receptor-dependent plasticity, by modifying inhibitory-signalling. In addition, Zif268 may actively suppress a novel plasticity-related transcription factor, urma. These findings may underlie important processes in learning and memory.
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Shilling, Paul D. "Chronic amphetamine treatment : behavioral responses,molecular mechanisms and differential regional Zif268 mRNA expression /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9835289.
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Maroteaux, Matthieu. "Implication du facteur de transcription Zif268 dans les apprentissages guidés par la récompense." Paris 6, 2010. http://www.theses.fr/2010PA066305.
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Les drogues détournent les circuits neuronaux de la récompense et y provoquent des altérations responsables des comportements d’addiction. Ces circuits impliquent les ganglions de la base (GB), qui s’organisent en boucles fonctionnelles formées par les voies directe et indirecte qui contrôlent l’activité de ses structures de sortie. Le point d’entrée des GB est le striatum (STR) qui recoit des afférences glutamatergiques du cortex, et des projections des neurones dopaminergiques. Une activation des récepteurs NMDA et D1 de la dopamine (D1R), déclenche la cascade de signalisation des ERK1/2 dans les neurones striataux de la voie directe et recrute ses cibles, dont le facteur de transcription Zif268 et active la DARPP-32. Tous deux ont des rôles cruciaux dans les effets des drogues. Nous avons cherché à comprendre les rôles de la DARPP-32 et de Zif268 dans les mécanismes d’apprentissage par la nourriture. Nous avons pour cela caractérisé l’expression de Zif268 après des séances d’apprentissage opérant pour de la nourriture dans les deux voies des GB grâce à des souris exprimant la GFP sous la dépendance du promoteur du D1R. Nous avons ensuite mis en évidence une motivation réduite pour ce comportement opérant chez des souris portant une DARPP-32 mutée, ainsi que chez des souris où Zif268 a été invalidé. De plus nous montrons que la surexpression de Zif268 dans le STR dorso-médian est corrélée avec la réponse opérante au début de l’apprentissage opérant. Nous montrons que la DARPP-32 et Zif268 sont des acteurs majeurs dans le système de la récompense. A l’avenir leur étude permettra de mieux comprendre la mise en place de comportements tels que l’addiction ou l’obésité.
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Czerny, Florian. "Development of Zinc-Finger-Based Artificial Restriction Endonucleases and Fluorescent Peptidyl Metal Sensors." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://hdl.handle.net/11858/00-1735-0000-002B-7CAB-3.
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Gros, Alexandra. "Neurogenèse adulte hippocampique : Rôle fonctionnel dans la mémoire épisodique et recrutement des nouveaux neurones lors de la mémorisation." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA11T051.
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La neurogenèse adulte du gyrus denté de l’hippocampe joue un rôle essentiel dans les processus mnésiques dépendants de l’hippocampe, mais son rôle dans des formes complexes de mémoire comme la mémoire épisodique n’a jamais été exploré. Le travail de cette thèse porte sur l’étude de l’implication des nouveaux neurones de l’hippocampe dans la mise en mémoire d’un souvenir épisodique à long terme. Nous avons développé une nouvelle tâche de mémoire épisodique reposant sur la présentation occasionnelle d’épisodes permettant d’encoder des informations de type « Quoi – Où – Dans quel contexte ». Nous montrons pour la première fois que les rats sont capables de se souvenir à très long terme de brefs épisodes de vie et d’utiliser cette mémoire d’une manière flexible. La caractérisation des profils de rétention permet d’accéder aux capacités individuelles de recollection des différents éléments du souvenir et montre que le rappel fiable de la mémoire épisodique nécessite l’intégrité de l’hippocampe et met en jeu un vaste réseau hippocampo-cortical dont l’activation est corrélée au rappel. Les performances de rats soumis à une irradiation focale de l’hippocampe montrent que la neurogenèse adulte hippocampique contribue de façon significative à la consolidation et au rappel fiable du souvenir épisodique. Ces résultats sont discutés dans le cadre d’une implication de la neurogenèse adulte dans la résolution de la mise en mémoire d’événements occasionnels dans le but de discriminer deux épisodes de vie proches, en lien avec les fonctions de séparation et de complétion de patterns de l’hippocampe. Par ailleurs, les mécanismes moléculaires qui sous-tendent le recrutement des nouveaux neurones lors d’un apprentissage restent inconnus. Nous avons analysé le rôle du gène immédiat précoce Zif268, acteur moléculaire essentiel dans les processus mnésiques, et montrons que ce gène joue un rôle crucial dans la sélection et le recrutement des nouveaux neurones lors de la mémorisation au cours de leur période critique d’intégration dans les réseaux neuronaux de l’hippocampe. Ce travail apporte des éléments nouveaux sur la participation des nouveaux neurones hippocampiques dans les processus mnésiques dans une situation à forte demande cognitive basée sur l’encodage d’une représentation intégrée et résolue d’événements occasionnels complexes, ainsi que sur les mécanismes qui sous-tendent leur recrutementAdult hippocampal neurogenesis plays a critical role in hippocampal-dependent memory, however its role in complex forms of memory such as episodic memory has not as yet been explored. The work presented in this thesis focuses on the issue of the involvement of newborn hippocampal neurons in long term episodic memory. We developed a new episodic memory task based on the presentation of occasional episodes allowing rats to encode “What – Where – In which context” information. We show for the first time that rats are able to remember on the long term brief past episodes of life and to use their episodic memory in a flexible manner. The characterization of retention profiles allows us to identify individual abilities in the recollection of the various elements of the memory and shows that episodic memory recall requires the integrity of the hippocampus and involves a hippocampo-cortical network, the activation of which correlates with recall performance. Performance of rats subjected to focal irradiation of the hippocampus shows that adult hippocampal neurogenesis contributes significantly to the consolidation and faithful recall of episodic memory. These results are discussed in the context of the implication of hippocampal newborn neurons in the resolution of memories of occasional events in order to discriminate different, but closely related episodes of life in relation to pattern separation and pattern completion functions of the hippocampus. Furthermore, the molecular mechanisms underlying the recruitment of newborn hippocampal neurons by learning remain to date unknown. We investigated the role of Zif268, an immediate early gene known to play an essential role in memory processes, and show that this gene plays a crucial role in the selection and recruitment of newborn hippocampal neurons by learning during their critical period of integration in hippocampal neural networks. Overall, this work brings new knowledge on the contribution of newborn hippocampal neurons to memory processes in a highly demanding cognitive situation based on the encoding of an integrated and high-resolution representation of complex occasional events, and on the mechanisms underlying their recruitment
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Gheidi, Ali, Erin Azzopardi, Allison Adams, and Diano Marrone. "Experience-dependent persistent expression of zif268 during rest is preserved in the aged dentate gyrus." BioMed Central, 2013. http://hdl.handle.net/10150/610073.
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BACKGROUND:Aging is typically accompanied by memory decline and changes in hippocampal function. Among these changes is a decline in the activity of the dentate gyrus (DG) during behavior. Lasting memory, however, is thought to also require recapitulation of recent memory traces during subsequent rest - a phenomenon, termed memory trace reactivation, which is compromised in hippocampal CA1 with progressive age. This process has yet to be assessed in the aged DG, despite its prominent role in age-related memory impairment. Using zif268 transcription to measure granule cell recruitment, DG activity in adult and aged animals was assessed both during spatial exploration and as animals remained at rest in the home cage in order to detect potential memory-related replay.RESULTS:Consistent with the observation of memory trace reactivation in DG, the probability that an individual granule cell transcribes zif268 during rest in the animal's home cage is increased by recent experience in a novel environment. Surprisingly, a comparable increase was observed in the probability of granule cells in the aged DG expressing zif268 during rest. Moreover, no significant age-related difference was observed in the number of granule cells expressing zif268 during rest. Thus, the number and pattern of granule cell expression of zif268 during rest is preserved in aged animals, despite a significant decline in exploration-related zif268 expression.CONCLUSIONS:These data lead to the hypothesis that the input the aged DG receives from backprojections from CA3 (the region widely hypothesized to mediate reactivation) remains functionally intact despite loss of innervation from the perforant path.
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Lee, Peter S. M. Massachusetts Institute of Technology. "Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/34490.
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Thesis (S.M.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006.Includes bibliographical references (p. 42-43).
Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of force on the single molecule. Measuring the force applied in real-time is dependent on detecting the bead's displacement from the trapping laser beam axis. Back-focal-plane detection provides a way of measuring the displacement, in two-dimensions, at nanometer or better resolution. The first part of this work will describe the design of a simple and inexpensive position sensing module customized for optical tweezers applications. Single molecule fluorescence is another powerful technique used to obtain microscopic details in biological systems. This technique can detect the arrival of a single molecule into a small volume of space or detect the conformational changes of a single molecule. Combining optical tweezers with single-molecule fluorescence so that one can apply forces on a single molecule while monitoring its effects via single molecule fluorescence provides an even more powerful experimental platform to perform such microscopic studies. Due to the enhanced photobleaching of fluorophores caused by the trapping laser, this combined technology has only been demonstrated under optimized conditions.
(cont.) The second part of this work will describe a straightforward and noninvasive method of eliminating this problem. The study of mechanotransduction in biological systems is critical to understanding the coupling between mechanical forces and biochemical reactions. Due to the recent advances in single molecule technology, it is now possible to probe such mechanisms at the single molecule level. The third and final part of this work will describe a basic mechanotransduction experiment using the well-studied ZIF268 protein-DNA system. An experimental assay and method of analysis will be outlined.
by Peter Lee.
S.M.
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Bozon, Bruno. "Implication du gène immédiat précoce zif268 dans la plasticité synaptique, la consolidation et la reconsolidation mnésique." Paris 11, 2003. http://www.theses.fr/2003PA112121.
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On admet généralement que la mémoire à long terme repose sur des d'efficacité synaptique dans les réseaux neuronaux activés par l'apprentissage et que cette plasticité dépend de régulations transcriptionnelles. Nous nous sommes attachés à caractériser le rôle du gène immédiat précoce zif268 dans la plasticité synaptique et dans les processus de consolidation et de reconsolidation mnésique, et de situer sa place dans la cascade de signalisation impliquant le facteur de transcription CREB, par l'utilisation de souris génétiquement modifiées. Des analyses électrophysiologiques montrent que l'invalidation du gène zif268 n'altère pas la transmission synaptique dans le gyrus denté de l'hippocampe, mais que la potentialisation à long terme (LTP) ne peut se maintenir au-delà de 24h, suggérant que zif268 est nécessaire au maintien des phases tardives de la LTP. L'étude de différentes formes de mémoires montre que l'invalidation de zif268 empêche la formation d'une mémoire à long terme dans des tâches spatiales et non spatiales, sans altérer la mémoire à court terme. Ce déficit de consolidation est soumis à un effet de dosage génique dépendant du type d'apprentissage mais peut cependant être compensé par un apprentissage distribué et intensif. Nos résultats montrent aussi que l'inactivation fonctionnelle de CREB chez des souris transgéniques conditionnelles, comme l'inactivation de zif268, provoque un déficit de mémoire de reconnaissance à long terme. Enfin, nous avons examiné l'implication de zif268 dans les processus de reconsolidation d'une mémoire de reconnaissance après rappel. En l'absence de zif268, lorsque l'on réactive une trace précédemment consolidée, cette dernière n'est pas reconsolidée, conduisant à un déficit de rappel ultérieur. Ces résultats mettent en évidence le rôle crucial du gène zif268 dans le maintien à long terme de la plasticité synaptique hippocampique ainsi que dans la consolidation et à la reconsolidation mnésiqueIt is a commonly accepted premise that long-term memory is based on persistent changes in synaptic strength in neuronal networks activated by learning and that plasticity relies on transcriptional regulation. Here we have focused on the potential roles primarily of the immediate early gene zif268 and to a lesser extent, the transcription factor CREB, in long-lasting synaptic plasticity and in the processes of memory consolidation and reconsolidation, using genetically modified mice. Our electrophysiological analyses show that invalidation of zif268 does not impair synaptic transmission in the dentate gyrus of the hippocampus, but that long-term potentiation (LTP) does not persist beyond 24h, suggesting zif268 is necessary for the maintenance of the late phases of LTP. Our behavioural experiments show that invalidation of zif268 prevents the formation of a long-term memory in both spatial and non-spatial memory tasks, without effecting short-term memory. This consolidation deficit relies on a task-dependent gene-dose effect, but the deficit can be compensated for by an extended and distributed learning. Our results also show that functional inactivation of CREB in conditional transgenic mice leads, as shown with inactivation of zif268, to long-term recognition memory impairment. Finally, we examined the implication of zif268 in the process of reconsolidation of recognition memory after reactivation of the memory trace. Although with extended training zif268 mutant mice have normal recognition memory when tested 5 days later, if the memory trace is reactivated after learning they are unable to reconsolidate the information, leading to a deficit in later recall. These results show a critical role of zif268 in the maintenance of long-term synaptic plasticity in the hippocampus and in memory consolidation and reconsolidation under optimal conditions
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Santos, Jos? Ronaldo dos. "Express?o de zif268 no c?rebro do lagarto Tropidurus Hispidus ap?s explora??o de um ambiente enriquecido." Universidade Federal do Rio Grande do Norte, 2010. http://repositorio.ufrn.br:8080/jspui/handle/123456789/17291.
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Previous issue date: 2010-02-24Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
In the present work, we investigated behavioral changes associated with the increase in Zif268 protein expression within telencephalic areas of the tropical lizard Tropidurus hispidus that correspond to the mammalian hippocampus (HC). We used 13 male individuals of this species, collected at the Federal Agrotechnical School of Rio Grande do Norte, under SISBIO license number 19561-1. Four animals had their brains removed and were submitted to a Western blot with antibodies for the Zif268 protein. The remaining animals were separated in two different groups: a control group (n=4) and an exploration group (n=5). Animals from the exploration group were exposed to an enriched environment with many sensory cues novel to them. Control group animals stayed in the environment they were already habituated to. After 90 min from the onset of exposure to the new environment, animals from both groups were submitted to intracardiac perfusion with fixative, and the brains were removed, cryoprotected and frozen. After that, brains were sectioned at 20 μm and the sections were subjected to immunohistochemistry for the Zif268 protein. We verified that the Zif268 protein is likely conserved in the brain of T. hispidus, which showed antigenicity for the antibody anti-Zif268 made in mammals. In animals from the exploration group, we detected an increase of the Zif268 protein in the Septum, Striatum, Dorsoventricular Area and in cortical areas corresponding to the HC. This increase was proportional to the amount of environmental exploration, with maximum positive correlation in the hippocampal subareas Medial Cortex (R = 0.94 and p = 0.004) and Dorsomedial Cortex (R = 0.92 and p = 0.006). The data corroborate the notion that the reptilian hippocampus, as well as the mammalian HC, plays an important role in spatial exploration.
Neste trabalho, foram investigadas altera??es comportamentais associadas ao aumento da express?o da prote?na Zif268 em ?reas telencef?licas do lagarto tropical Tropidurus hispidus correspondentes ao Hipocampo (HC) de mam?feros. Foram utilizados 13 animais machos do lagarto T. hispidus, coletados no campus da Escola Agrot?cnica Federal do RN, sob a licen?a do SISBIO (n.19561-1). Quatro animais tiveram seus c?rebros removidos a fresco e submetidos a Western blot com anticorpo para a prote?na Zif268. Os animais restantes foram separados em dois grupos distintos, grupo controle (n=4) e grupo explora??o (n=5). Animais do grupo explora??o foram expostos a um Ambiente Enriquecido (AE) com diversas pistas espaciais desconhecidas pelos animais. Os animais do grupo controle permaneceram no ambiente ao qual j? estavam previamente habituados. Transcorridos 90min do in?cio da exposi??o ao ambiente, animais de ambos os grupos, foram submetidos a perfus?o intracard?aca com fixador, e os c?rebros foram removidos crioprotegidos e congelados. Posteriormente os c?rebros foram seccionados a 20μm e submetidos ? imunohistoqu?mica para Zif268. Verificamos a conserva??o da prote?na Zif268 no c?rebro do T. hispidus, com antigenicidade para o anticorpo anti-Zif268 produzido em mam?feros. Nos lagartos do grupo explora??o detectou-se aumento da express?o da prote?na Zif268 no Septo, Estriado, ?rea Dorsoventricular e ?reas corticais que correspondem ao HC (C?rtices Medial CM, Dorsal CD, Dorsomedial CDM). Esse aumento ? proporcional ? explora??o do ambiente novo, com m?xima correla??o nas sub?reas hipocampais do grupo explora??o, C?rtex Medial (R = 0,94 e p = 0,004) e Dorsomedial (R = 0,92 e p = 0,006). Os dados corroboram a no??o de que o hipocampo reptiliano, assim como o HC de mam?feros, desempenham um papel importante na explora??o de novos ambientes.
Books on the topic "Zif268"
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Metal ligation in ZIF268, a zinc finger protein: Effects on DNA binding. Ottawa: National Library of Canada, 1996.
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Roxtrom, Git. Towards Simulating Fully Charged Protein-DNA Systems in Water: Zif268-DNA As a Test Case (Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 492). Uppsala Universitet, 1999.
Find full textBook chapters on the topic "Zif268"
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Chen, Yashun, Fangfang He, Wei Liu, and Jucheng Zhang. "Spectroscopic Studies on the Interaction of Pyridinoline Cross-Linking in Type 1 Collagen with ZIF8-HB." In Advances in Experimental Medicine and Biology, 63–67. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-38810-6_9.
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Veyrac, Alexandra, Antoine Besnard, Jocelyne Caboche, Sabrina Davis, and Serge Laroche. "The Transcription Factor Zif268/Egr1, Brain Plasticity, and Memory." In Progress in Molecular Biology and Translational Science, 89–129. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-12-420170-5.00004-0.
Full textConference papers on the topic "Zif268"
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Zhang, Daohong. "A paper-based device for double-stranded DNA detection with Zif268." In MATERIALS SCIENCE, ENERGY TECHNOLOGY, AND POWER ENGINEERING I: 1st International Conference on Materials Science, Energy Technology, Power Engineering (MEP 2017). Author(s), 2017. http://dx.doi.org/10.1063/1.4982427.
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