Academic literature on the topic 'ZTGC DNA'

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Journal articles on the topic "ZTGC DNA"

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Czernecki, Dariusz, Haidai Hu, Filippo Romoli та Marc Delarue. "Structural dynamics and determinants of 2-aminoadenine specificity in DNA polymerase DpoZ of vibriophage ϕVC8". Nucleic Acids Research 49, № 20 (2021): 11974–85. http://dx.doi.org/10.1093/nar/gkab955.

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Abstract All genetic information in cellular life is stored in DNA copolymers composed of four basic building blocks (ATGC-DNA). In contrast, a group of bacteriophages belonging to families Siphoviridae and Podoviridae has abandoned the usage of one of them, adenine (A), replacing it with 2-aminoadenine (Z). The resulting ZTGC-DNA is more stable than its ATGC-DNA counterpart, owing to the additional hydrogen bond present in the 2-aminoadenine:thymine (Z:T) base pair, while the additional amino group also confers resistance to the host endonucleases. Recently, two classes of replicative protein
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Gao, Shuliang, Huiwen Guan, Hanan Bloomer, et al. "Harnessing non-Watson–Crick’s base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cells." Proceedings of the National Academy of Sciences 121, no. 2 (2023). http://dx.doi.org/10.1073/pnas.2308415120.

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Genomic DNA of the cyanophage S-2L virus is composed of 2-aminoadenine (Z), thymine (T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates Watson–Crick base pairing rules. The Z-base has an extra amino group on the two position that allows the formation of a third hydrogen bond with thymine in DNA strands. Here, we explored and expanded applications of this non-Watson–Crick base pairing in protein expression and gene editing. Both ZTGC-DNA (Z-DNA) and ZUGC-RNA (Z-RNA) produced in vitro show detectable compatibility and can be decoded in mammalian cells, including
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Czernecki, Dariusz, Frédéric Bonhomme, Pierre-Alexandre Kaminski, and Marc Delarue. "Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA." Nature Communications 12, no. 1 (2021). http://dx.doi.org/10.1038/s41467-021-25064-x.

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AbstractCyanophage S-2L is known to profoundly alter the biophysical properties of its DNA by replacing all adenines (A) with 2-aminoadenines (Z), which still pair with thymines but with a triple hydrogen bond. It was recently demonstrated that a homologue of adenylosuccinate synthetase (PurZ) and a dATP triphosphohydrolase (DatZ) are two important pieces of the metabolism of 2-aminoadenine, participating in the synthesis of ZTGC-DNA. Here, we determine that S-2L PurZ can use either dATP or ATP as a source of energy, thereby also depleting the pool of nucleotides in dATP. Furthermore, we ident
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Dissertations / Theses on the topic "ZTGC DNA"

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Czernecki, Dariusz. "Discovery and structure-function studies of key factors behind the non-canonical ZTGC-DNA observed in Siphoviridae family." Electronic Thesis or Diss., Sorbonne université, 2020. http://www.theses.fr/2020SORUS199.

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Le but de cette thèse est de décrire le chemin métabolique permettant de remplacer l’adénine (A) par la 2-aminoadénine (Z) dans le génome de bactériophages Siphoviridae. La 2-aminoadénine et la thymine (T) forment la paire Z:T, liée par trois liaisons hydrogène. Avec la paire G:C classique, ils forment un ADN « ZTGC » qui est résistant aux enzymes de réstriction de l’hôte. En premier lieu, mes travaux se sont focalisés sur le cyanophage S-2L, décrit comme porteur de 2-aminoadénine. J’ai d’abord étudié la primase-polymérase, PrimPol, responsable de la réplication de l’ADN chez ces phages, et qu
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Book chapters on the topic "ZTGC DNA"

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Oliveira, Daniele S., Alexander Sachse, Vinícius P. S. Caldeira, and Sibele B. C. Pergher. "VALORIZAÇÃO DO GLICEROL PARA A SÍNTESE DE MATERIAIS NANOESTRUTURADOS À BASE DE CARBONO UTILIZANDO ZEÓLITAS: UMA REVISÃO." In Anais do II Congresso da Rede Brasileira de Bioquerosene e Hidrocarbonetos Sustentáveis de Aviação. Editora Científica Digital, 2023. http://dx.doi.org/10.37885/230914444.

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O excesso de glicerol como subproduto do biodiesel, tornou-se um obstáculo na cadeia de produção de biodiesel, criando novos desafios para seu uso sustentável. Com isso, a obtenção de produtos de maior valor agregado deve ser estudada para melhorar a viabilidade econômica da indústria de biodiesel e valorizar o glicerol. Este trabalho apresenta uma ideia/sugestão de utilização do glicerol como precursor na obtenção de carbonos microporosos com molde de zeólita (Zeolite Templated Carbon - ZTC). Apresentando as vantagens e oportunidades da obtenção de ZTC com glicerol. Apesar de interessante, es
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