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Dissertations / Theses on the topic 'Zygotic embryos'

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Published: 4 June 2021

Last updated: 26 July 2025

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1

Lucas, Tanguy. "Spatio-temporal regulation of hunchback during the zygotic genome activation in Drosophila." Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066707.

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Les gradients morphogénétiques contrôlent l'émergence de polarités axiales au cours du développement. Bien que la dynamique d'établissement de ces gradients soit bien comprise, la précision des mécanismes d'activation agissant en aval restent à élucider. Nous abordons cette question avec le gradient de Bicoid qui fournit rapidement une réponse transcriptionnelle robuste dans l'embryon de drosophile. Cette robustesse survient malgré le challenge imposé par de fréquentes mitoses dépourvues de transcription. Un calcul théorique intégrant les paramètres physiques de Bicoid (concentration, diffusio

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2

Lucas, Tanguy. "Spatio-temporal regulation of hunchback during the zygotic genome activation in Drosophila." Electronic Thesis or Diss., Paris 6, 2015. http://www.theses.fr/2015PA066707.

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Les gradients morphogénétiques contrôlent l'émergence de polarités axiales au cours du développement. Bien que la dynamique d'établissement de ces gradients soit bien comprise, la précision des mécanismes d'activation agissant en aval restent à élucider. Nous abordons cette question avec le gradient de Bicoid qui fournit rapidement une réponse transcriptionnelle robuste dans l'embryon de drosophile. Cette robustesse survient malgré le challenge imposé par de fréquentes mitoses dépourvues de transcription. Un calcul théorique intégrant les paramètres physiques de Bicoid (concentration, diffusio

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3

Laurie, John Drummond. "In vitro production and long term storage of maize zygotic embryos derived from isolated embryo sacs." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq22624.pdf.

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4

Ozkurt, Zeynep. "Induction Of Embryogenic Tissue From Immature Zygotic Embryos In Pinus Nigra Subspecies Pallasiana Lamb." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/12607615/index.pdf.

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Cloning of trees using somatic embryogenesis could have a major impact on tree breeding and commercial plantation forestry. To initiate somatic embryogenesis in Anatolian black pine (Pinus nigra Arnold. subspecies pallasiana), one-year old cones containing immature seeds were collected from eight trees located in METU campus, Ankara. Embryogenic tissues were derived from immature zygotic embryos excised from the seeds. The zygotic embryos at the time of collection were at the precotyledonary stage of development. For this study, Douglas-fir cotyledon revised medium (DCR) supplemented with 13.6

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5

Santanen, Arja. "Polyamine metabolism during development of somatic and zygotic embryos of Picea abies (Norway spruce)." Helsinki : University of Helsinki, 2000. http://ethesis.helsinki.fi/julkaisut/mat/bioti/vk/santanen/.

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6

Lopes, Carla Aparecida Ragonezi Gomes. "In vitro plantlet regeneration from mature zygotic embryos of Pinus pinea L.: overcoming the rooting problems." Doctoral thesis, Universidade de Évora, 2013. http://hdl.handle.net/10174/16017.

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Regeneração in vitro de plântulas de embriões zigóticos maduros de Pinus pinea L.: superando os problemas de enraizamento Na natureza, as plantas e os microrganismos estabelecem associações de várias ordens. Nas culturas in vitro de plantas, mesmo as associações favoráveis com microrganismos foram, durante muitos anos, consideradas como contaminantes. Só mais tarde, as vantagens da inoculação in vitro (co-cultura) foram demonstradas e as técnicas de biotização (bacterização ou micorrização) usadas com o objetivo de melhorar as condições de crescimento in vitro. As dificuldades do enraizamento

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7

Hovey, Kathryn Marie Carleton University Dissertation Biology. "The detection of glucosinolates during development of Brassica juncea (var. RLM 514) anther-derived and zygotic embryos." Ottawa, 1990.

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8

Morrow, Carl. "Cryopreservation of Podocarpus elongatus (Ait.) L'Herit. ex Pers. and Podocarpus falcatus (Thunb.) R. Br. ex Mirb. zygotic embryos." Thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/25771.

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9

Aye, Felicia Yeboaa Okraku. "Cultural and environmental effects on lipid accumulation during embryogenesis of zygotic and somatic embryos of Theobroma cacao L. cultured in vitro." Thesis, University of Reading, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269743.

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10

Almerei, Ayman. "Agrobacterium-mediated transformation of Syrian maize with anti-stress genes." Thesis, University of Plymouth, 2016. http://hdl.handle.net/10026.1/5336.

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Agrobacterium is widely considered, when suitably modified, to be the most effective vector for gene transfer into plant cells. For a long time, many cereals crops (monocotyledonous plants) were recalcitrant species to genetic modification, mainly as a result of their recalcitrance to in-vitro regeneration and their resistance to Agrobacterium infection. However, recently Agrobacterium-mediated transformation has been used to transform monocot crops such as maize (Zea mays) but with severe restrictions on genotype suitability. This study was carried out to evaluate the transformation amenabili

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11

Machado, Caroline de Araújo. "Estratégias para conservação de acessos de coqueiro anão do BAG da Embrapa Tabuleiros Costeiros." Universidade Federal de Sergipe, 2012. http://ri.ufs.br:8080/xmlui/handle/123456789/3100.

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The conservation of the coconut palm has been investigated in recent years by research organizations, public and private. These studies have concentrated on obtaining conservation protocols in vitro using slow growth and cryopreservation. Storage as a means of maintaining the viability of pollen, it is important for the preservation of genetic variability, facilitates the exchange of germplasm and contributes greatly to the generation of variability obtained from artificial crosses increasing the efficiency of breeding programs. The objective of this work was to study the effect of mannitol an

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12

Shah, Sheetal Mansukhlal. "Genetic and molecular studies of segmentation and axon guidance in Drosophila." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312177.

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13

Pires, Camilla Valente. "Regulação gênica dos processos iniciais do desenvolvimento de embriões haploides e diploides de Apis mellifera." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-21052014-090558/.

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O desenvolvimento embrionário é o resultado de uma sequência controlada de eventos modulados por sinais ambientais e mecanismos intracelulares. Em Hymenoptera, esse processo tem um caráter especial devido ao sistema de determinação do sexo (Haplodiploide). Neste sistema, os ovos fecundados se desenvolvem em fêmeas (diploides) e os ovos não fecundados em machos (haploides). Assim, eventos importantes, como a ativação do ovo e transição materno-zigótica, eventos iniciais da embriogênese, são elementos-chave para compreender o desenvolvimento de ambos os tipos de embriões. Ativação do ovo é um ev

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14

Papadopoulos, George. "Chromosome anomalies in human lymphocytes, oocytes, zygotes and preimplantation embryos." Thesis, University of Aberdeen, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278882.

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Chromosome aberrations are very important in human life because they can lead to disease or death. They have three important dimensions, formation, transmission and effect. They may occur spontaneously or be induced by physical or chemical agents. Human reproductive failure is characterised by a high rate of spontaneous chromosome anomalies while chromosome aberrations in human cancers are in most cases induced. In a proliferating cell system chromosome aberrations may be eliminated or transmitted for one or more cell divisions. In the former case the daughter cells may revert to normality or

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15

Zhu, Meng. "Investigating the mechanisms and the temporal regulation of the first cell polarity establishment in the mouse embryo." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/288353.

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Embryonic cells of many species polarise and the cell polarity is often important for the normal developmental progression. In the mouse embryo, the prototype of epithelial cell polarity, namely apico-basal polarisation, become established at the 2.5 days' post-fertilisation, when the embryos are at the 8-cell stage. The formation of apical domain is necessary and sufficient for the first segregation of extra-embryonic and embryonic cell lineages, as well as the following up morphogenetic transitions, such as the blastocyst formation. This study aims to explore the molecular pathways triggerin

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16

Rosfelter, Anne. "Le positionnement du fuseau mitotique chez le zygote d'ascidie et son rôle dans la répartition des organelles." Electronic Thesis or Diss., Sorbonne université, 2023. http://www.theses.fr/2023SORUS063.

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Après la fécondation d’un ovocyte, un aster de microtubules se forme autour de l’ADN mâle. Cet aster spermatique permet d’amener le pro-noyau femelle jusqu’au pro-noyau mâle pour qu’ils puissent fusionner. Il permet aussi de déplacer l’ADN fusionné jusqu’au centre de la cellule pour assurer une division cellulaire équitable. Les mécanismes de centration d’un aster ou d’un fuseau ont donné lieu à de nombreuses recherches, que ce soit par modélisation, expérimentalement chez des espèces telles C. elegans, P. lividus, M.musculus ou in vitro sur des extraits de Xenopus laevis. Trois mécanismes pri

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17

Morel, Alexandre. "Physiologie moléculaire du développement des embryons somatiques de pin maritime (Pinus pinaster Ait.) : approches transcriptomique et protéomique." Phd thesis, Université d'Orléans, 2014. http://tel.archives-ouvertes.fr/tel-01069408.

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Chez le pin maritime l'embryogenèse somatique, méthode de multiplication végétative performante, n'est pas optimisée la limite étant le contrôle du développement des embryons somatiques (ES). Nos objectifs ont été 1) d'étudier les mécanismes physiologiques, cellulaires et moléculaires précoces contrôlant la différenciation des ES en réponse à une disponibilité en eau réduite 2) d'évaluer pour l'ES cotylédonaire de 12 semaines son état de maturité, son protéome afin de les comparer à l'embryon zygotique (EZ). 1) Pour le 1er objectif, le rapprochement de données de transcriptomique et de protéom

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18

Salah, Wed Mohammed K. "Impact of Assisted Reproductive Technology on the Expression of Epigenetic Marker Histone 3 Lysine 9 in Zygotes." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29256.

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Normal zygotic maturation entails significant chromatin remodelling and epigenetic reprogramming of parental genomes to provide the framework for embryonic genome activation during the second cell cycle. Embryonic genome activation is required for proper embryonic development and involves a shift from maternal to embryonic regulation of gene transcription. Activation of the embryonic genome necessitates the transformation of chromatin from a transcriptionally repressed to active state. This event is disrupted by environmental factors such as culture in vitro, resulting in decreased embryonic v

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19

Zhu, Cuihua. "Serine palmitoyltransferase and ceramide kinase in embryo development of loblolly pine." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/22671.

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Thesis (Ph. D.)--Biology, Georgia Institute of Technology, 2008.<br>Committee Chair: Cairney, John; Committee Member: Merrill, Alfred Jr.; Committee Member: Nichols, Wylie; Committee Member: Pullman, Jerry; Committee Member: Streelman,Todd.

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20

Ryu, Junghyun. "The direct injection of CRISPR/Cas9 system into porcine zygotes for genetically modified pig production." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/101763.

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The pig has similar features to the human in aspects such as physiology, immunology, and organ size. Because of these similarities, genetically modified pigs have been generated for xenotransplantation. Also, when using the pig as a model for human diseases (e.g. cystic fibrosis transmembrane conductance regulator), the pig exhibited similar symptoms to those that human patients present. The main goal of this work was to examine the efficacy of direct injection of the CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/ CRISPR associated protein 9) in pigs and to over

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21

Dunlap-Brown, Marya. "The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes." Thesis, Virginia Tech, 2010. http://hdl.handle.net/10919/33743.

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Pronuclear stage murine embryos received electrical stimulation in 5, 10, or 20 Âµs pulse lengths, and 0, 100, 200, 250, 300 or 400 voltages. Minimal embryo development occurred with 400 V. Irreversible electroporation occurred in embryos electroporated for 5 Âµs pulse length at 100 and 400 V, 10 Âµs pulse length at 400 V, and 20 Âµs pulse length at 100, 250, 300 and 400 V. Electroporated embryos that underwent reversible electroporation received 100 V for 5 Âµs, 400 V for 10 Âµs, and 250 for 20 Âµs and had similar development (P > 0.05) between the best and worst developed groups. Enhan

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22

Berger, Frédéric. "Quelques aspects de la biologie cellulaire du développement précoce (polarisation, embryogenèse) du zygote de Fucus." Lyon 1, 1994. http://www.theses.fr/1994LYO1T310.

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23

Dutta, Rahul Verfasser], Angelika [Akademischer Betreuer] [Gutachter] [Schnieke, and Wilhelm M. [Gutachter] Windisch. "Genome editing in livestock : optimisation of porcine embryo culture condition for direct zygotic manipulation / Rahul Dutta. Betreuer: Angelika Schnieke. Gutachter: Wilhelm M. Windisch ; Angelika Schnieke." München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/1103135392/34.

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24

Hajdu, Melissa Anne. "Effect of culture conditions, donor source, and injection site on in vitro development of deoxyribonucleic acid microinjected porcine zygotes." Thesis, This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-12172008-063219/.

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25

Flinn, Barry Stanley. "Storage protein gene expression in zygotic and somatic embryos of interior spruce." Thesis, 1992. http://hdl.handle.net/2429/2914.

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Storage proteins from interior spruce (Picea glauca/engelmanhi complex) were identified, partially characterized and used as markers to compare the developmental fidelity between zygotic and somatic embryos. The major storage proteins expressed in both embryo types had molecular weights of approximately 41, 35, 33, 24 and 22 kD. The 41 kD protein was buffer and low salt-soluble, whereas the 35-33 kD and 24-22 kD proteins were high salt-soluble and disulfide linked. All of the proteins possessed several isoelectric variants. Based on solubility and disulfide linkage characteristics, as

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26

Ragonezi, Carla. "In vitro plantlet regeneration from mature zygotic embryos of Pinus pinea L.: overcoming the rooting problems." Doctoral thesis, 2013. http://hdl.handle.net/10400.13/4066.

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Na natureza, as plantas e os microrganismos estabelecem associações de várias ordens. Nas culturas in vitro de plantas, mesmo as associações favoráveis com microrganismos foram, durante muitos anos, consideradas como contaminantes. Só mais tarde, as vantagens da inoculação in vitro (co-cultura) foram demonstradas e as técnicas de biotização (bacterização ou micorrização) usadas com o objetivo de melhorar as condições de crescimento in vitro. As dificuldades do enraizamento in vitro de uma das espécies mais importantes da floresta mediterrânica portuguesa, Pinus pinea L., conduziu

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27

Arnold, Marianne. "An Analysis of the Development of Shoot Apices in Excised Immature Zygotic Cotton Embryos (Gossypium hirsutum cv Texas Marker-1)." Thesis, 2011. http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10638.

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Although cottonseed is an important source of oil and fiber, the development of cotton embryos has not been investigated as well as development of cotton fiber. The development of cotton embryos in late heart-stage and early cotyledonary stage is less well investigated than the first 10-14 days after anthesis, or the late stages of embryo development during seed-fill and desiccation. This analysis focused on cotton embryos in the late heart-stage and early cotyledonary stage of development (1.5-4.0 mm or about 13-18 DPA). In vitro analyses are important tools for studying embryos in isolat

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28

Naidoo, Sershen. "Studies on factors influencing viability after cryopreservation of excised zygotic embryos from recalcitrant seeds of two amaryllid species." Thesis, 2010. http://hdl.handle.net/10413/5571.

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Recalcitrant unlike orthodox seeds do not show a sharp border between maturation and germination and remain highly hydrated and desiccation-sensitive at all developmental and post-harvest stages. In contrast with recalcitrant seeds, orthodox types retain viability for predictably long periods in the dry state and hence can be stored under low relative humidity and temperature conditions. Storage of recalcitrant seeds under conditions allowing little to no water loss, at moderate temperatures, allows for short- to medium-term storage but only facilitates viability retention for a matter of a fe

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29

Ngobese, Nomali Ziphorah. "Effects of some of the procedural steps of cyropreservation on cryo-recalcitrant zygotic embryos of three amaryllid species producing desiccation-sensitive seeds." Thesis, 2013. http://hdl.handle.net/10413/11223.

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Cryopreservation is the most promising method for the long-term conservation of germplasm of plants producing desiccation-sensitive seeds. While such seeds are generally termed recalcitrant in the context of conventional storage practices, the term ‘cryo-recalcitrant’ is used for germplasm which is not readily amenable to cryopreservation. Cryo procedures usually involve a sequential combination of steps which must be optimised to limit the stresses experienced by specimens, thus promoting their survival. The present contribution reports on the effects of some of the steps involved in cryopres

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30

Ganesh, Sravya. "Dlouhé nekódující RNA během přeměny vajíčka na embryo." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-391390.

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(English) Oocyte-to-embryo transition (OET) is one of the most complex developmental events, during which a differentiated oocyte gives rise to a totipotent zygote. During OET a transcriptionally silent oocyte undergoes massive reprogramming of gene expression, which transforms it into a transcriptionally active zygote. Although numerous studies have contributed to understanding the mechanism of OET, many genes involved in OET are yet to be identified. A whole new level of possible regulation of OET came with the discovery of long non-coding RNAs (lncRNA). LncRNAs are pol II transcripts longer

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31

Lin, Hui-Ju, and 林惠茹. "Zygotic embryo somatic embryogenesis and hypocotyl organogenesis in Psidium guajava L." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/82450005087211004309.

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碩士<br>國立屏東科技大學<br>農園生產系所<br>100<br>Guava (Psidium guajava L.) is a tropical fruit which belongs to the family Myrtaceae and the genus Psidium. It is an important economical fruit crop in Taiwan. To establish an efficient regeneration system for guava micropropagation, zygotic embryos for somatic embryogenesis and hypocotyls for organogenesis were used in this study. Among zygotic embryos with different week-old ages, the best induction rate of somatic embryogenesis was achieved with 10 week-old zygotic embryos from summer fruits. Regardless the week-old ages of zygotic embryos, the best in

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32

Eid, Laila N. "Paternal regulation of the zygotic cell cycle in the bovine embryo." 1995. http://catalog.hathitrust.org/api/volumes/oclc/34415376.html.

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33

Zuccaro, Michael V. "Genome stability in the preimplantation embryo." Thesis, 2021. https://doi.org/10.7916/d8-kqyg-zm58.

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The mammalian zygote and resulting embryo is the starting point of life, and thus must overcome continuous insult from DNA stress and damage while maintaining genome stability and integrity. This thesis examines genome stability in the context of chromosome changes, both in the context of ploidy and whole genome duplications as well as double-strand DNA breakage and chromosome loss. Regarding the ploidy portion of this work, while possible to derive and maintain, mammalian haploid stem cells undergo spontaneous, irreversible diploidization. Here, we investigated the mechanisms driving

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34

Kräußling, Michael. "Analysis on division patterns and transcriptional activity in embryos from medaka "Oryzias latipes" before the midblastula transition." Doctoral thesis, 2011. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-66911.

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Das Studium der Entwicklung von Tieren ist eine der ältesten Disziplinen in der Biologie. Die gesammelten Daten von unzähligen Untersuchungen an den verschiedensten Spezies wurden dazu benutzt, um ein generelles Verständnis des tierischen Lebenszykluses zu formulieren. Ein wichtiges Ergebnis der intensiven Untersuchungen war vor etwa einem Jahrhundert die Entdeckung spezifischer morphologischer Veränderungen, die sich während der Teilungsphase, der Zeitperiode die der Befruchtung und Aktivierung des Eies am Anfang der Embryogenese folgt, vollziehen. Diese Befunde führten schlussendlich zur For

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35

Nghiem, QC. "Effects of ploidy level on the reproductive biology of tropical Acacia species." Thesis, 2012. https://eprints.utas.edu.au/16138/10/Nghiem_whole_thesis_ex_pub_mat.pdf.

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Acacia mangium Willd., A. auriculiformis A. Cunn. ex Benth and their natural hybrid, A. mangium x A. auriculiformis, are important plantation species for timber production in the tropics. However, their potential to invade natural ecosystems has been a concern because of their prolific production of long-lived seeds. Deployment of triploid Acacia clones in plantations might reduce fertility and associated weed risk. Studies were conducted in a hybridizing orchard that was established in southern Vietnam in 2003 with alternate rows of diploid A. mangium (AM-2x), diploid A. auriculiformis (AA-2

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Tsagareli, Victoria. "The effects of short-term energy restriction in overweight/obese females on reproductive outcomes." Thesis, 2008. http://hdl.handle.net/2440/47585.

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In the general population, one in five couples experiences difficulty in conceiving a child. The role of obesity on women’s fecundity has become a focus of attention in recent years. Successful treatment of infertility through Assisted Reproductive Technology (ART) is also compromised by the presence of obesity, which occurs in 30 % of women seeking treatment. A negative correlation exists between increased body mass index (BMI) and the number of collected oocytes and a lower birth rate after ART. Furthermore, a number of studies have established that weight loss improves natural conception r

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37

Martin, Jacinta. "Quality control mechanisms responsible for the maintenance of genomic integrity in the female germline." Thesis, 2019. http://hdl.handle.net/1959.13/1395720.

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Research Doctorate - Doctor of Philosophy (PhD)<br>DNA is the genetic repository containing the necessary information for cellular viability, fate decisions and development. In the female germline, genetic integrity also underpins successful conception, embryonic development, pregnancy and the future health of the offspring. In spite of its importance, DNA remains a chemical entity prone to structural alteration. If left unresolved, these structural lesions have the potential to lead to mutation and broader-scale genomic aberrations, which may elevate the predisposition of individuals to non-c

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38

Tsagareli, Victoria. "The effects of short-term energy restriction in overweight/obese females on reproductive outcomes." 2008. http://hdl.handle.net/2440/47585.

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In the general population, one in five couples experiences difficulty in conceiving a child. The role of obesity on women’s fecundity has become a focus of attention in recent years. Successful treatment of infertility through Assisted Reproductive Technology (ART) is also compromised by the presence of obesity, which occurs in 30 % of women seeking treatment. A negative correlation exists between increased body mass index (BMI) and the number of collected oocytes and a lower birth rate after ART. Furthermore, a number of studies have established that weight loss improves natural conception ra

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