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Literatura académica sobre el tema "Production de mutants de trypanosomes"

Autor: Grafiati

Publicado: 25 de enero de 2025

Última modificación: 31 de julio de 2025

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Artículos de revistas sobre el tema "Production de mutants de trypanosomes"

Villafraz, Oriana, Marc Biran, Erika Pineda, et al. "Procyclic trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in the presence of physiological amounts of proline." PLOS Pathogens 17, no. 3 (2021): e1009204. http://dx.doi.org/10.1371/journal.ppat.1009204.

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Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), is transmitted by the tsetse fly where the procyclic forms of the parasite develop in the proline-rich (1–2 mM) and glucose-depleted digestive tract. Proline is essential for the midgut colonization of the parasite in the insect vector, however other carbon sources could be available and used to feed its central metabolism. Here we show that procyclic trypanosomes can consume and metabolize metabolic intermediates, including those excreted from glucose catabolism (succinate, alanine and pyruvate),

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Olayinka-Adefemi, Folayemi, Chukwunonso Onyilagha, Nipun Jayachandran та ін. "The Function of Phosphatidylinositol 3-Kinase delta (PI3Kδ) Enzyme in Protective Immunity to Trypanosoma congolense Infection in Mice: The Role of Regulatory B cells". Journal of Immunology 204, № 1_Supplement (2020): 82.39. http://dx.doi.org/10.4049/jimmunol.204.supp.82.39.

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Abstract The delta isoform of PI 3-kinase (PI3Kδ) has important functions in B cell activation. Trypanosoma parasites utilize several mechanisms to exploit and evade host B cell and antibody responses critical for immunity. We sought to determine the impact of PI3Kδ in immunity to Trypanosoma congolense infection. We found that PI3KδD910A mutant mice show a surprisingly enhanced control of parasitemia in early infection (7–9 days) when compared to wild-type (WT) C57BL/6 mice. Drug treatment with Idelalisib to acutely inhibit PI3Kδ during infection in WT mice led to improved early control of pa

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Beneke, Tom, Ross Madden, Laura Makin, Jessica Valli, Jack Sunter, and Eva Gluenz. "A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids." Royal Society Open Science 4, no. 5 (2017): 170095. http://dx.doi.org/10.1098/rsos.170095.

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Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids. CRISPR technology provides the opportunity to develop scalable methods for high-throughput production of mutant phenotypes. Here, we report development of a CRISPR-Cas9 toolkit that allows rapid tagging and gene knockout in diverse kinetoplastid species without requiring the user to perform any DNA cloning. We developed a new protocol for single-guide RNA (sgRNA) delivery using PCR-generated DNA t

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Sass, Gabriele, Laura C. Miller Conrad, Terrence-Thang H. Nguyen, and David A. Stevens. "The Pseudomonas aeruginosa product pyochelin interferes with Trypanosoma cruzi infection and multiplication in vitro." Transactions of The Royal Society of Tropical Medicine and Hygiene 114, no. 7 (2020): 492–98. http://dx.doi.org/10.1093/trstmh/trz136.

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Abstract Background Bacteria are sources of numerous molecules used in treatment of infectious diseases. We investigated effects of molecules produced by 26 Pseudomonas aeruginosa strains against infection of mammalian cell cultures with Trypanosoma cruzi, the aetiological agent of Chagas disease. Methods Vero cells were infected with T. cruzi in the presence of wild-type P. aeruginosa supernatants or supernatants of mutants with defects in the production of various virulence, quorum sensing and iron acquisition factors. Quantification of T. cruzi infection (percentage of infected cells) and m

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Vassella, Erik, Peter Bütikofer, Markus Engstler, Jennifer Jelk, and Isabel Roditi. "Procyclin Null Mutants ofTrypanosoma bruceiExpress Free Glycosylphosphatidylinositols on Their Surface." Molecular Biology of the Cell 14, no. 4 (2003): 1308–18. http://dx.doi.org/10.1091/mbc.e02-10-0694.

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Procyclins are abundant, glycosylphosphatidylinositol (GPI)-anchored proteins on the surface of procyclic (insect) form trypanosomes. To investigate whether trypanosomes are able to survive without a procyclin coat, all four procyclin genes were deleted sequentially. Bloodstream forms of the null mutant exhibited no detectable phenotype and were able to differentiate to procyclic forms. Initially, differentiated null mutant cells were barely able to grow, but after an adaptation period of 2 mo in culture they proliferated at the same rate as wild-type trypanosomes. Analysis of these culture-ad

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Lüscher, Alexandra, Pinar Önal, Anne-Marie Schweingruber, and Pascal Mäser. "Adenosine Kinase of Trypanosoma brucei and Its Role in Susceptibility to Adenosine Antimetabolites." Antimicrobial Agents and Chemotherapy 51, no. 11 (2007): 3895–901. http://dx.doi.org/10.1128/aac.00458-07.

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ABSTRACT Trypanosoma brucei cannot synthesize purines de novo and relies on purine salvage from its hosts to build nucleic acids. With adenosine being a preferred purine source of bloodstream-form trypanosomes, adenosine kinase (AK; EC 2.7.1.20) is likely to be a key player in purine salvage. Adenosine kinase is also of high pharmacological interest, since for many adenosine antimetabolites, phosphorylation is a prerequisite for activity. Here, we cloned and functionally characterized adenosine kinase from T. brucei (TbAK). TbAK is a tandem gene, expressed in both procyclic- and bloodstream-fo

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Mensa-Wilmot, K., JH LeBowitz, KP Chang, et al. "A glycosylphosphatidylinositol (GPI)-negative phenotype produced in Leishmania major by GPI phospholipase C from Trypanosoma brucei: topography of two GPI pathways." Journal of Cell Biology 124, no. 6 (1994): 935–47. http://dx.doi.org/10.1083/jcb.124.6.935.

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The major surface macromolecules of the protozoan parasite Leishmania major, gp63 (a metalloprotease), and lipophosphoglycan (a polysaccharide), are glycosylphosphatidylinositol (GPI) anchored. We expressed a cytoplasmic glycosylphosphatidylinositol phospholipase C (GPI-PLC) in L. major in order to examine the topography of the protein-GPI and polysaccharide-GPI pathways. In L. major cells expressing GPI-PLC, cell-associated gp63 could not be detected in immunoblots. Pulse-chase analysis revealed that gp63 was secreted into the culture medium with a half-time of 5.5 h. Secreted gp63 lacked ant

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Guerra-Giraldez, Cristina, Luis Quijada, and Christine E. Clayton. "Compartmentation of enzymes in a microbody, the glycosome, is essential in Trypanosoma brucei." Journal of Cell Science 115, no. 13 (2002): 2651–58. http://dx.doi.org/10.1242/jcs.115.13.2651.

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All kinetoplastids contain membrane-bound microbodies known as glycosomes,in which several metabolic pathways including part of glycolysis are compartmentalized. Peroxin 2 is essential for the import of many proteins into the microbodies of yeasts and mammals. The PEX2 gene of Trypanosoma brucei was identified and its expression was silenced by means of tetracycline-inducible RNA interference. Bloodstream-form trypanosomes, which rely exclusively on glycolysis for ATP generation, died rapidly upon PEX2 depletion. Insect-form (procyclic) trypanosomes do not rely solely on glycolysis for ATP syn

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Lillico, Simon, Mark C. Field, Pat Blundell, Graham H. Coombs, and Jeremy C. Mottram. "Essential Roles for GPI-anchored Proteins in African Trypanosomes Revealed Using Mutants Deficient in GPI8." Molecular Biology of the Cell 14, no. 3 (2003): 1182–94. http://dx.doi.org/10.1091/mbc.e02-03-0167.

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The survival of Trypanosoma brucei, the causative agent of Sleeping Sickness and Nagana, is facilitated by the expression of a dense surface coat of glycosylphosphatidylinositol (GPI)-anchored proteins in both its mammalian and tsetse fly hosts. We have characterized T. brucei GPI8, the gene encoding the catalytic subunit of the GPI:protein transamidase complex that adds preformed GPI anchors onto nascent polypeptides. Deletion ofGPI8 (to give Δgpi8) resulted in the absence of GPI-anchored proteins from the cell surface of procyclic form trypanosomes and accumulation of a pool of non–protein-l

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Ralston, Katherine S., Neville K. Kisalu, and Kent L. Hill. "Structure-Function Analysis of Dynein Light Chain 1 Identifies Viable Motility Mutants in Bloodstream-Form Trypanosoma brucei." Eukaryotic Cell 10, no. 7 (2011): 884–94. http://dx.doi.org/10.1128/ec.00298-10.

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ABSTRACT The flagellum of Trypanosoma brucei is an essential and multifunctional organelle that is receiving increasing attention as a potential drug target and as a system for studying flagellum biology. RNA interference (RNAi) knockdown is widely used to test the requirement for a protein in flagellar motility and has suggested that normal flagellar motility is essential for viability in bloodstream-form trypanosomes. However, RNAi knockdown alone provides limited functional information because the consequence is often loss of a multiprotein complex. We therefore developed an inducible syste

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Tesis sobre el tema "Production de mutants de trypanosomes"

El, Kadri Mohammad. "Role(s) of glycerol metabolism in the biology of African trypanosomes." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0456.

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Trypanosoma brucei, un parasite extracellulaire responsable de la trypanosomiase africaine, doit s’adapter à différents environnements dans ses hôtes mammifères et l’insecte vecteur (la mouche tsétsé). Dans le sang des mammifères, le glucose est la principale source de carbone soutenant une croissance rapide du parasite, en alimentant son métabolisme et la production d'ATP. Lorsque les formes sanguines prolifératives « slender » atteignent des densités cellulaires élevées, un quorum sensing induit leur différenciation en formes non réplicatives « stumpy » (stumpy-QS). Ces formes stumpy-QS prot

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Fitzek, Elisabeth. "Production and characterization of mutants of UDP-glucose pyrophosphorylase." Thesis, Umeå University, Plant Physiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-26081.

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<p>UDP-glucose pyrophosphorylase (UGPase) is a key component of carbohydrate production in plants, especially with respect to sucrose synthesis/ metabolism, by producing UDP-glucose, a key precursor to sucrose and to many polysaccharides in cell walls. UDP-glucose is also utilized in the synthesis of carbohydrate moiety of glycolipids, glycoproteins and a variety of secondary metabolites, among other functions. The UGPase enzyme may have a rate-limiting function in sugar biosynthesis, and its activity is now known to increase upon variety of abiotic stresses, with possible effects on an overal

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Niazy, Abdurahman. "Virulence Factor Production in PyrE Mutants of Pseudomonas Aeruginosa." Thesis, University of North Texas, 2010. https://digital.library.unt.edu/ark:/67531/metadc28458/.

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It has been shown previously in our lab that mutations in the pyrimidine pathway reduced the ability of Pseudomonas aeruginosa to produce virulence factors. Knockout mutations in pyrB, pyrC and pyrD genes of the pyrimidine pathway showed that virulence factor production was decreased. Pyoverdin, pyocyanin, hemolysin, iron chelation, motility, and adherence are all considered virulence factors. Here I further investigate the effects of mutations in the pyrimidine pathway by studying a pyrE mutant. I studied the effect of the pyrE mutation on the production of the above virulence factors. Just l

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Kuriakose, Shiby. "Molecular regulation of Trypanosoma congolense-induced proinflammatory cytokine production in macrophages and its modulation by diminazene aceturate (Berenil)." PLOS, Frontiers in Immunology, Elsevier, Sage, 2016. http://hdl.handle.net/1993/31677.

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African trypanosomiasis remains a major health problem to both humans and animals due to lack of effective treatment or vaccine to control the disease. Animal trypanosomiasis is considered one of the most important diseases affecting livestock production and agricultural development in sub-Saharan Africa. Although the use of trypanocides remain the most important method for controlling the disease in animals, the mechanisms of action of these compounds are not completely known. The overall aim of this thesis is to decipher the molecular mechanisms involved in Trypanosoma congolense-induced cyt

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Zouheiry, Hassane. "La pénicilline amidase d'Escherichia coli : recherche des meilleurs conditions de production par bactéries recombinées, mise au point de production et de caractérisation de mutants, étude de ces mutants." Nancy 1, 1991. http://www.theses.fr/1991NAN10021.

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Ce travail, réalisé en collaboration avec Rhône-Poulenc santé, a consisté d'une part, à étudier la régulation de l'expression du gène cloné de la pénicilline amidase (PA) dans une souche mutante, d'autre part, à développer une technique de mutagenèse chimique adaptée à l'étude d'enzyme, à l'appliquer au gène PA et à caractériser les mutants obtenus. La souche transformée présente les caractéristiques suivantes par rapport à la souche non transformée: une exigence plus importante en source de carbone, un effet non pas négatif mais positif du glucose sur la production de PA, une expression en pa

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Zeyniyev, Araz. "Cephamycin C Production By Streptomyces Clavuligerus Mutants Impaired In Regulation Of Aspartokinase." Master's thesis, METU, 2006. http://etd.lib.metu.edu.tr/upload/12607641/index.pdf.

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Aspartokinase is the first enzyme of the aspartate family amino acids biosynthetic pathway. Cephamycin C is a &amp<br>#946<br>-lactam antibiotic produced as a secondary metabolite via the enzymatic reactions in the lysine branch of this pathway in Streptomyces clavuligerus. The aspartokinase activity of S. clavuligerus is under concerted feedback inhibition by two of the end product amino acids, lysine plus threonine. It is also known that carbon flow through the lysine branch of the aspartate pathway is rate limiting step in the formation of cephamycin C. Therefore, genetic alterations in th

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Liu, Xiaoguang. "Production of butyric acid and hydrogen by metabolically engineered mutants of Clostridium tyrobutyricum." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1124114266.

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Thesis (Ph. D.)--Ohio State University, 2005.<br>Title from first page of PDF file. Document formatted into pages; contains xv, 220 p.; also includes graphics (some col.). Includes bibliographical references (p. 189-201). Available online via OhioLINK's ETD Center

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Wahab, Adbul. "Regulation of antibiotic production in Streptomyces coelicolor : phenotypic and transcriptomic analysis of AbsA mutants." Thesis, University of Surrey, 2007. http://epubs.surrey.ac.uk/843422/.

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The AbsA two-component system is a negative regulator of antibiotic biosynthesis in Streptomyces coelicolor. To gain further insight into the role of the AbsA system different in-frame deletion mutations were made in the absA1A2 operon in S. coelicolor MT1110, MT1110DeltacdaR and S. lividans 1326. Phenotypic analysis of DeltaabsA1A2 and DeltaabsA2 in the MT1110 derivatives showed that CDA was produced early on Oxoid nutrient agar relative to the parent strain. The absA mutants in S. lividans 1326 (both DeltaabsA1 and DeltaabsA1A2) did not show early CDA production. On solid R2YE both MT1110Del

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Xue, Liu. "HIGH-ACTIVITY MUTANTS OF HUMAN BUTYRYLCHOLINESTERASE FOR COCAINE ABUSE TREATMENT." UKnowledge, 2013. http://uknowledge.uky.edu/pharmacy_etds/40.

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Cocaine is a widely abused drug without an FDA-approved medication. It has been recognized as an ideal anti-cocaine medication to accelerate cocaine metabolism producing biologically inactive metabolites via a route similar to the primary cocaine-metabolizing pathway, i.e. butyrylcholinesterase (BChE)-catalyzed hydrolysis. However, the native BChE has a low catalytic activity against cocaine. We recently designed and discovered a set of BChE mutants with a high catalytic activity specifically for cocaine. An ideal, therapeutically valuable mutant of human BChE should have not only a significan

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Merah, Mostefa. "Obtention et étude de mutants de Myxococcus xanthus affectés dans la production d'activités enzymatiques exocellulaires." Rouen, 1992. http://www.theses.fr/1992ROUES017.

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Myxococcus xanthus est une bactérie à gram-négatif qui sécrète de nombreuses activités enzymatiques durant la phase exponentielle de croissance. Nous avons tenté dans une première étape de cloner un gène de structure de l'une des trois activités sécrétées par cette bactérie: protéase, endoprotéase acide et glucanase en utilisant le promoteur mobile Ptac permettant de créer des phénotypes conditionnels inductibles par l'IPTG. Les résultats que nous avons obtenus ont montré que le clonage d'un fragment d'ADN de M xanthus situé en aval du Ptac dans E. Coli est faisable. Mais l'obtention condition

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Libros sobre el tema "Production de mutants de trypanosomes"

Simpson, Duncan Robert. Characterisation of morphological mutants arising during production of Quorn myco-protein. University of Manchester, 1996.

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Stephens, Sarah Katharine. The production and characterisation of extracellular enzyme mutants in Coprinus bilanatus. University of Birmingham, 1988.

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Ikushujō, Hōshasen, ed. Production of mutants in tree crops: Report of symposium held on July 20-21, 1989. Institute of Radiation Breeding, NIAR MAFF, 1991.

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Capítulos de libros sobre el tema "Production de mutants de trypanosomes"

Moguilevsky, N., F. Varsalona, J. P. Guillaume, C. Roobol, and A. Bollen. "Recombinant Human Proapolipoprotein A-I: Experimental Strategies for the Production of an Authentic Molecule." In Human Apolipoprotein Mutants III. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84634-2_14.

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Sarsu, Fatma, Suprasanna Penna, and Ganesh C. Nikalje. "Strategies for Screening Induced Mutants for Stress Tolerance." In Mutation Breeding for Sustainable Food Production and Climate Resilience. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-16-9720-3_6.

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Awazuhara, Motoko, Akio Furuhashi, Toru Fujiwara, and Mitsuo Chino. "Screening of Arabidopsis thaliana mutants sensitive to sulfur deficiency." In Plant Nutrition for Sustainable Food Production and Environment. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0047-9_54.

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Park, Gyungsoon, Hildur V. Colot, Patrick D. Collopy, et al. "High-Throughput Production of Gene Replacement Mutants in Neurospora crassa." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-040-9_13.

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Nishiwaki, Toshikazu, Takashi Sato, Hiroyuki Yashima, et al. "Changes in concentration of leghemoglobin components in hypernodulation mutants of soybean." In Plant Nutrition for Sustainable Food Production and Environment. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0047-9_222.

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Bakshi, Suman, Johar Singh, and Sanjay J. Jambhulkar. "Isolation and characterization of yellow rust resistant mutants in wheat." In Mutation breeding, genetic diversity and crop adaptation to climate change. CABI, 2021. http://dx.doi.org/10.1079/9781789249095.0010.

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Abstract Stripe rust, also known as yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat production leading to yield losses up to 84%. Due to climate change, new races of the yellow rust pathogen are appearing for which no durable source of resistance has been observed in the present high-yielding varieties. A mutation breeding programme was initiated in two popular varieties, namely PBW343 and HD2967, using gamma-ray and electron beam irradiation. Gamma-ray doses of 250, 300 and 350 Gy and electron beam doses of 150, 200 and 250 Gy were used for seed ir

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Pühler, A., M. F. Hynes, D. Kapp, P. Müller, and K. Niehaus. "Infection Mutants of Rhizobium Meliloti are Altered in Acidic Exopolysaccharide Production." In Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71652-2_3.

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Ruengjitchatchawalya, M., S. Chamutpong, M. Ponglikitmongkol, R. Chaiklahan, and M. Tanticharoen. "Spirulina Platensis Mutants Defective in γ-Linolenic Acid Production: Molecular characterization." In Advanced Research on Plant Lipids. Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0159-4_23.

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Faull, J. L., and K. Graeme-Cook. "Characterization of Mutants of Trichoderma Harzianum with Altered Antibiotic Production Characteristics." In Biological Control of Plant Diseases. Springer US, 1992. http://dx.doi.org/10.1007/978-1-4757-9468-7_48.

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Alvarez, W. Navarro. "Production of salt tolerant rice mutants using gamma rays and anther culture." In Mutations, In Vitro and Molecular Techniques for Environmentally Sustainable Crop Improvement. Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-015-9996-2_9.

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Actas de conferencias sobre el tema "Production de mutants de trypanosomes"

Solarte, Ana Lucía, Rafael Jesús Astorga, Fabiana de Aguiar, Alfonso Maldonado, Ángela Galán-Relaño, and Belén Huerta. "Induction of resistant mutants of Salmonella typhimurium under enrofloxacin and natural alternatives for control in pigs." In Safe Pork 2015: Epidemiology and control of hazards in pork production chain. Iowa State University, Digital Press, 2017. http://dx.doi.org/10.31274/safepork-180809-380.

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Gammanpila, H. W., and K. R. Manjula. "Exploring the Influence of Intermittent Heat Exposure on Spontaneous Mutations in Drosophila melanogaster: Assessing the Role of Vitamin C in Mitigating Heat Stress and Examining Inheritance Patterns of Induced Mutations." In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/thuh5711.

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Climate change poses a significant threat to the well-being of organisms. It has a detrimental impact on the survival of smaller organisms in response to climatic shifts, posing a substantial danger to biodiversity, which is already under stress due to habitat loss, emerging invasive species, and diseases. This study aimed to assess the influence of fluctuating temperatures on the physiology and behavior of Drosophila melanogaster, as well as to investigate whether such temperature fluctuations have any effect on phenotypic expression through potential spontaneous mutations. Genotypic changes

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Alkotami, Linah, Brice Jarvis, Chaofu Lu, et al. "Targeted genome editing of industrial oilseed crops to enhance synthesis of functional lipids." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/orfd6797.

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Acetyl-triacylglycerols (acetyl-TAG) produced in the seeds of different Euonymus species are triacylglycerols (TAG) that possess an sn-3 acetate group instead of a long chain fatty acid. This unusual structure confers useful properties to acetyl-TAG, including reduced kinematic viscosity and improved cold temperature performance. Acetyl-TAG are synthesized by unique diacylglycerol acetyltransferases (DAcTs), expressed in the endosperm of Euonymus seeds, that use acetyl-CoA to acetylate the sn-3 position of diacylglycerol (DAG) molecules. Isolation and expression of DAcT enzymes from different

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Informes sobre el tema "Production de mutants de trypanosomes"

Ori, Naomi, and Jason W. Reed. Engineering parthenocarpic fruit production in tomato. United States-Israel Binational Agricultural Research and Development Fund, 2021. http://dx.doi.org/10.32747/2021.8134175.bard.

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Normally, fruits are formed only following fertilization. In tomato, fertilization is sensitive to extreme temperatures, resulting in reduced yield. Yield stability would therefore benefit from tomato varieties with parthenocarpic fruits, which develop independently of fertilization. The objective of the research was to generate parthenocarpic tomato lines by mutating PRC2 components, to investigate how PRC2 and auxin signaling regulate fruit initiation and growth, and to generate parthenocarpic lines for breeding. We reasoned that heterozygous prc2 mutations would generate parthenocarpic frui

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Horwitz, Benjamin A., and Barbara Gillian Turgeon. Fungal Iron Acquisition, Oxidative Stress and Virulence in the Cochliobolus-maize Interaction. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7709885.bard.

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Our project focused on genes for high affinity iron acquisition in Cochliobolus heterostrophus, a necrotrophic pathogen of maize, and their intertwined relationship to oxidative stress status and virulence of the fungus on the host. An intriguing question was why mutants lacking the nonribosomal peptide synthetase (NRPS) gene (NPS6) responsible for synthesis of the extracellular siderophore, coprogen, are sensitive to oxidative stress. Our overall objective was to understand the mechanistic connection between iron stress and oxidative stress as related to virulence of a plant pathogen to its h

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Sooksai, Sarintip, and Kobkul Laoteng. Genetic study of fatty acid and lipid biosynthesis in Hansenula polymorpha. Chulalongkorn University, 2011. https://doi.org/10.58837/chula.res.2011.121.

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Two groups of Hansenula polymorpha mutants were screened by their fatty acid requirement, one requires saturated fatty acids for growth (sfa- mutamts) and the other requires unsaturated fatty acids (mfa- and pfa- mutants). Two of the sfa- mutants, S7 and S16, showed significantly difference in the fatty acid composition. S7 clearly defected in the production of C18:2∆9,12 and C18:3∆9,12,15, while S16 significantly accumulated medium-chain saturated fatty acids, C12:0 and C14:0. By tetrad analysis, the results showed that S7 had double mutation which composed of fatty acid synthesis mutation (H

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Eshel, Amram, Jonathan P. Lynch, and Kathleen M. Brown. Physiological Regulation of Root System Architecture: The Role of Ethylene and Phosphorus. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585195.bard.

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Specific Objectives and Related Results: 1) Determine the effect of phosphorus availability on ethylene production by roots. Test the hypothesis that phosphorus availability regulates ethylene production Clear differences were found between the two plants that were studied. In beans ethylene production is affected by P nutrition, tissue type, and stage of development. There are genotypic differences in the rate of ethylene production by various root types and in the differential in ethylene production when P treatments are compared. The acceleration in ethylene production with P deficiency inc

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Gantt, Elisabeth, Avigad Vonshak, Sammy Boussiba, Zvi Cohen, and Amos Richmond. Carotenoid-Rich Algal Biomass for Aquaculture: Astaxanthin Production by Haematococcus Pluvialis. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613036.bard.

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The synthesis of carotenoids has been studied toward enhancing the production of ketocarotenoids, since fish and crustaceans raised by aquaculture require astaxanthin and other ketocaroteinoids in their feed for desirable pigmentation. Notable progress has been made in attaining the goals of determining improved conditions for ketocarotenoid production in Haematococcus pluvialis and in elucidating the carotenoid biosynthetic pathway. For production of astaxanthin a number of strains of the green alga Haematococcus were evaluated, a strain CCAG was found to be optimal for photoautotrophic growt

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Antignus, Yehezkiel, Ernest Hiebert, Shlomo Cohen, and Susan Webb. Approaches for Studying the Interaction of Geminiviruses with Their Whitefly Vector Bemisia tabaci. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604928.bard.

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The DNA of tomato yellow leaf curl virus (TYLCB) was detected in its whitefly vector, Bemisia tabaci, by dot spot hybridization as early as 1 h after acquisition access. The retention of the virus nucleic acid in the vector was at least 23 days after a 48 h acquisition access. However, the retention of TYLCV coat protein did not exceed 10 days. No replicative forms of TYLCV could be detected in B. tabaci, indicating a non-propagative relationship with the vector. Whiteflies were not able to accumulate naked virion ssDNA, virus cloned dsDNA, or virions with impaired coat protein. Deletion, fram

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Prusky, Dov, Noel T. Keen, and Stanley Freeman. Elicitation of Preformed Antifungal Compounds by Non-Pathogenic Fungus Mutants and their Use for the Prevention of Postharvest Decay in Avocado Fruits. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7570573.bard.

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C. gloeosporioides attacks unripe avocado fruits in the orchard. Germinated spores produce appressoria that germinate and breach the cuticle, but the resultant subcuticular hyphae become quiescent and do not develop further until fruit is harvested and ripens. Resistance of unripe avocado to attach by C. gloeosporioides is correlated with the presence of fungitoxic concentrations of the preformed antifungal compound, 1-acetoxy-2-hydroxy-4-oxoheneicosa-12, 15 diene in the pericarp of unripe fruits. The objective of this proposal was to study the signal transduction process by which elicitors in

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Alfano, James, Isaac Barash, Thomas Clemente, Paul E. Staswick, Guido Sessa, and Shulamit Manulis. Elucidating the Functions of Type III Effectors from Necrogenic and Tumorigenic Bacterial Pathogens. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592638.bard.

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Many phytopathogenic bacteria use a type III protein secretion system (T3SS) to inject type III effectors into plant cells. In the experiments supported by this one-year feasibility study we investigated type III effector function in plants by using two contrasting bacterial pathogens: Pseudomonas syringae pv. tomato, a necrotrophic pathogen and Pantoea agglomerans, a tumorigenic pathogen. The objectives are listed below along with our major conclusions, achievements, and implications for science and agriculture. Objective 1: Compare Pseudomonas syringae and Pantoea agglomerans type III effect

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Lindow, Steven, Yedidya Gafni, Shulamit Manulis, and Isaac Barash. Role and In situ Regulation of Growth Regulators Produced in Plant-Microbe Interactions by Erwinia herbicola. United States Department of Agriculture, 1992. http://dx.doi.org/10.32747/1992.7561059.bard.

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The main objective of this work was to gain a better understanding of how some strains of Erwinia herbicola have evolved into serious plant pathogens while also commonly existing as epiphytes on the surface of healthy plants. The focus of our studies was to determine the nature of, and regulation, of virulence factors, including the phytohormones IAA and cytokinins, which are encoded on a large plasmid (pPATH) found in gall-forming strains of this species. In addition, the in situ regulation and contribution to epiphytic fitness of a second, chromosomal, IAA biosynthetic locus (ipdC) was deter

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Splitter, Gary A., Menachem Banai, and Jerome S. Harms. Brucella second messenger coordinates stages of infection. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7699864.bard.

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Aim 1: To determine levels of this second messenger in: a) B. melitensiscyclic-dimericguanosinemonophosphate-regulating mutants (BMEI1448, BMEI1453, and BMEI1520), and b) B. melitensis16M (wild type) and mutant infections of macrophages and immune competent mice. (US lab primary) Aim 2: To determine proteomic differences between Brucelladeletion mutants BMEI1453 (high cyclic-dimericguanosinemonophosphate, chronic persistent state) and BMEI1520 (low cyclicdimericguanosinemonophosphate, acute virulent state) compared to wild type B. melitensisto identify the role of this second messenger in esta

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