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Статті в журналах з теми "TnAg"

Автор: Grafiati

Опубліковано: 15 вересня 2021

Оновлено: 30 липня 2025

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1

hÓgáin, Fionnán Ó. "Bíodh TnaG Againn!" Comhar 51, no. 5 (1992): 220. http://dx.doi.org/10.2307/25571800.

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2

Sedlacek, Abigail L., David Osei-Hwedieh, Archibald Agyekum-Yamoah, et al. "Identification of immunogenic peptide neoantigens expressed in sarcomas and their therapeutic potential." Journal of Immunology 208, no. 1_Supplement (2022): 179.12. http://dx.doi.org/10.4049/jimmunol.208.supp.179.12.

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Abstract T cells recognize tumor neoantigens (TNag) in the context of MHC and eliminate cells which express these antigens, leaving those lacking immunodominant epitopes. Central to investigating tumor immunoediting is the ability to define immunogenic TNag. Differential aggretope index (DAI) is a measure of the difference in MHC binding affinity between mutated and unmutated peptides and has emerged as a highly efficient way of identifying immunogenic TNag. Our previous studies have demonstrated that in mice, TNag with a DAI &gt;8 are considered immunogenic and elicit T cells that lead to

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3

Feinneadha, Ciarán Ó. "TnaG - an ród a bhí romhainn." Comhar 54, no. 5 (1995): 10. http://dx.doi.org/10.2307/25572644.

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4

de Fréine, Celia. "A Soap for TnaG: Writing “ Ros na Rún ”." New Hibernia Review 3, no. 1 (1999): 142–48. http://dx.doi.org/10.1353/nhr.1999.a926667.

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5

Konan, Kouacou Vincent, and Charles Yanofsky. "Role of Ribosome Release in Regulation oftna Operon Expression in Escherichia coli." Journal of Bacteriology 181, no. 5 (1999): 1530–36. http://dx.doi.org/10.1128/jb.181.5.1530-1536.1999.

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ABSTRACT Expression of the degradative tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. In cultures growing in the absence of added tryptophan, transcription of the structural genes of the tna operon is limited by Rho-dependent transcription termination in the leader region of the operon. Tryptophan induction prevents this Rho-dependent termination, and requires in-frame translation of a 24-residue leader peptide coding region, tnaC, that contains a single, crucial, Trp codon. Studies with a lacZ reporter

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6

Tovey, Hilary. "Book Review: Comparison of the Campaigns for Raidio na Gaeltachta and TnaG." Irish Journal of Sociology 11, no. 2 (2002): 147–49. http://dx.doi.org/10.1177/079160350201100209.

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7

Rafian, T., and Y. Yurnalis. "Polymorphism of Insulin-Like Growth Factor-1 (IGF-1) Gene on Bayang Ducks Using PCR-RFLP Method." Jurnal Sain Peternakan Indonesia 18, no. 3 (2023): 162–68. http://dx.doi.org/10.31186/jspi.id.18.3.162-168.

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This study aims to determine the polymorphism of the IGF-1 gene in Bayang ducks using the PCR-RFLP method. This study used 120 blood samples of Bayang ducks in Kampung Binuang Dalam, Padang City, West Sumatra Province. Bayang duck blood samples were taken through the brachial vein for ± 1 mL. DNA extraction was taken from the blood using the Genomic DNA Purification Kit (Promega) Protocol. DNA was amplified using a pair of primers F: 5'- CCA GGA ATA TCT TTG GAA GCT GT-3 and R: 5'- TGC TAC GTT ACC AGC CTT GA -3 'which produced a 433 bp fragment of exon 3 IGF-1 gene, and F: 5'- CTG GAG CAG GCA G

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8

Konan, Kouacou Vincent, and Charles Yanofsky. "Rho-Dependent Transcription Termination in the tnaOperon of Escherichia coli: Roles of the boxASequence and the rut Site." Journal of Bacteriology 182, no. 14 (2000): 3981–88. http://dx.doi.org/10.1128/jb.182.14.3981-3988.2000.

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ABSTRACT Expression of the tryptophanase (tna) operon ofEscherichia coli is regulated by catabolite repression and by tryptophan-induced transcription antitermination. Tryptophan induction prevents Rho-dependent transcription termination in the leader region of the operon. Induction requires translation of a 24-residue leader peptide-coding region, tnaC, containing a single, crucial Trp codon. Studies with a lacZ reporter construct lacking the tnaC-tnaA spacer region suggest that, in the presence of excess tryptophan, the TnaC leader peptide acts incis on the ribosome translating tnaC to inhib

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9

Manicardi, G. C., D. Bizzaro, E. Galli, and U. Bianchi. "Heterochromatin heterogeneity in the holocentric X chromatin of Megoura viciae (Homoptera, Aphididae)." Genome 39, no. 2 (1996): 465–70. http://dx.doi.org/10.1139/g96-059.

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Holocentric chromosomes, prepared by spreading embryo cells obtained from Megoura viciae parthenogenetic females, have been C-banded, enzymatically digested in situ using the specific endonucleases DdeI (C↓TNAG), DraI (TTT↓AAA), Tru9I (TT↓AA), and CfoI (GCG↓C), and subsequently stained with Giemsa, DAPI, CMA3, and AgNO3. We observed that the X chromosome had the best defined banding patterns. In the M. viciae X chromosome there is a certain amount of heterogeneity in heterochromatic DNA composition. In fact, the GC-rich NOR-associated heterochromatin differs from other heterochromatic bands th

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10

Almansour, Haidara, Johann Jacoby, Heiko Baumgartner, Marie K. Reumann, Konstantin Nikolaou, and Fabian Springer. "Injury of the Tibial Nutrient Artery Canal during External Fixation for Lower Extremity Fractures: A Computed Tomography Study." Journal of Clinical Medicine 9, no. 7 (2020): 2235. http://dx.doi.org/10.3390/jcm9072235.

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The tibial nutrient artery (TNA) is the major diaphyseal artery of the tibia supplying two thirds of the inner osseous cortex. Hence, iatrogenic injury of the TNA endangers the integrity of the tibial blood supply and may compromise fracture healing. The incidence of its injury in the setting of external fixation for lower limb fractures has not been previously investigated. The aim of this study was to evaluate the incidence of TNA injury in the context of external fixation and to characterize the topography of the fixator pins in relation to the TNA canal (TNAC). Patients who underwent exter

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11

Cruz-Vera, Luis R., and Charles Yanofsky. "Conserved Residues Asp16 and Pro24 of TnaC-tRNAPro Participate in Tryptophan Induction of tna Operon Expression." Journal of Bacteriology 190, no. 14 (2008): 4791–97. http://dx.doi.org/10.1128/jb.00290-08.

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ABSTRACT In Escherichia coli, interactions between the nascent TnaC-tRNAPro peptidyl-tRNA and the translating ribosome create a tryptophan binding site in the ribosome where bound tryptophan inhibits TnaC-tRNAPro cleavage. This inhibition delays ribosome release, thereby inhibiting Rho factor binding and action, resulting in increased tna operon transcription. Replacing Trp12 of TnaC with any other amino acid residue was previously shown to prevent tryptophan binding and induction of tna operon expression. Genome-wide comparisons of TnaC amino acid sequences identify Asp16 and Pro24, as well a

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12

Cruz-Vera, Luis R., Rui Yang, and Charles Yanofsky. "Tryptophan Inhibits Proteus vulgaris TnaC Leader Peptide Elongation, Activating tna Operon Expression." Journal of Bacteriology 191, no. 22 (2009): 7001–6. http://dx.doi.org/10.1128/jb.01002-09.

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ABSTRACT Expression of the tna operon of Escherichia coli and of Proteus vulgaris is induced by l-tryptophan. In E. coli, tryptophan action is dependent on the presence of several critical residues (underlined) in the newly synthesized TnaC leader peptide, WFNIDXXL/IXXXXP. These residues are conserved in TnaC of P. vulgaris and of other bacterial species. TnaC of P. vulgaris has one additional feature, distinguishing it from TnaC of E. coli; it contains two C-terminal lysine residues following the conserved proline residue. In the present study, we investigated l-tryptophan induction of the P.

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13

Johnson, Morgan B., Emil F. Khisamutdinov, Kirill A. Afonin, and Ian Marriott. "Engineered immunomodulatory activity in therapeutic nucleic acids." Journal of Immunology 198, no. 1_Supplement (2017): 199.21. http://dx.doi.org/10.4049/jimmunol.198.supp.199.21.

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Abstract Therapeutic nucleic acids (TNAs) can be engineered to deliver functional moieties such as siRNAs to target cells. An additional benefit of TNAs is that they can be complexed with amphiphilic molecules for delivery into sites such as the central nervous system (CNS) that are difficult to access due to the blood-brain barrier. However, previous TNA constructs have failed clinical trials due to the induction of severe systemic inflammatory responses. We have developed a new generation of TNAs in which immunomodulatory activity, in addition to chemical and thermodynamic stability, can be

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14

Gong, Ming, Luis R. Cruz-Vera, and Charles Yanofsky. "Ribosome Recycling Factor and Release Factor 3 Action Promotes TnaC-Peptidyl-tRNA Dropoff and Relieves Ribosome Stalling during Tryptophan Induction of tna Operon Expression in Escherichia coli." Journal of Bacteriology 189, no. 8 (2007): 3147–55. http://dx.doi.org/10.1128/jb.01868-06.

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ABSTRACT Upon tryptophan induction of tna operon expression in Escherichia coli, the leader peptidyl-tRNA, TnaC- \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(tRNA_{2}^{Pro}\) \end{document} , resists cleavage, resulting in ribosome stalling at the tnaC stop codon. This stalled ribosome blocks Rho factor binding and action, preventing transcription termination in the tna operon's leader region. Plasmid-mediated overexpression of tnaC was previously shown to inhibit cell growth by re

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15

AL-BARZINJI, YOUSIF M. S. "MOLECULAR ANALYSIS OF FECGH GENE IN HAMDANI SHEEP BREED IN IRAQI KURDISTAN REGION." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 53, no. 1 (2022): 1. http://dx.doi.org/10.36103/ijas.v53i1.1498.

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The objective of this study was to investigate association between FecGH-GDF-9 gene polymorphism and litter size in Hamdani fat-tailed sheep. The genomic DNA was extracted from eighty-seven blood samples of Hamdani ewes. The FecGH-GDF-9 locus was detected by PCRs, and identified DNA genotyped by DNA sequencing. The evaluate several parameters included fertility (%), conception (%), letter size at birth, twining rate (%), triple rate (%), barrenness (%) and productivity (%) were measured and arrived 91.95, 95.40, 1.825, 150, 11.25, 8.05 and 159.77, respectively. The blast tree view results in N

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16

Allen, Randall, and Toshifumi Yokota. "Endosomal Escape and Nuclear Localization: Critical Barriers for Therapeutic Nucleic Acids." Molecules 29, no. 24 (2024): 5997. https://doi.org/10.3390/molecules29245997.

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Therapeutic nucleic acids (TNAs) including antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) have emerged as promising treatment strategies for a wide variety of diseases, offering the potential to modulate gene expression with a high degree of specificity. These small, synthetic nucleic acid-like molecules provide unique advantages over traditional pharmacological agents, including the ability to target previously “undruggable” genes. Despite this promise, several biological barriers severely limit their clinical efficacy. Upon administration, TNAs primarily enter cells thro

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17

Ren, Yanyu, Xiumin Shi, Pengcheng Xia, et al. "In Situ Raman Investigation of TiO2 Nanotube Array-Based Ultraviolet Photodetectors: Effects of Nanotube Length." Molecules 25, no. 8 (2020): 1854. http://dx.doi.org/10.3390/molecules25081854.

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TiO2 nanotube arrays (TNAs) with tube lengths of 4, 6, and 7 μm were prepared via two-step anodization. Thereafter, ultraviolet (UV) photodetectors (PDs) with Au/TiO2/Au structures were prepared using these TNAs with different tube lengths. The effects of TNA length and device area on the performance of the device were investigated using in situ Raman spectroscopy. The maximum laser/dark current ratio was achieved by using a TNA with a size of 1 × 1 cm2 and a length of 7 μm, under a 532 nm laser. In addition, when the device was irradiated with a higher energy laser (325 nm), the UV Raman spec

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18

Tallberg, Jonas, Thomas Sommerer, Theresa Squatrito, and Christer Jönsson. "Explaining the Transnational Design of International Organizations." International Organization 68, no. 4 (2014): 741–74. http://dx.doi.org/10.1017/s0020818314000149.

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AbstractPast decades have witnessed a shift in international cooperation toward growing involvement of transnational actors (TNAs), such as nongovernmental organizations, multinational corporations, and philanthropic foundations. This article offers a comprehensive theoretical and empirical account of TNA access to IOs. The analysis builds on a novel data set, covering formal TNA access to 298 organizational bodies from fifty IOs over the time period 1950 to 2010. We identify the most profound patterns in TNA access across time, issue areas, policy functions, and world regions, and statistical

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19

Cruz-Vera, Luis R., Aaron New, Catherine Squires, and Charles Yanofsky. "Ribosomal Features Essential for tna Operon Induction: Tryptophan Binding at the Peptidyl Transferase Center." Journal of Bacteriology 189, no. 8 (2007): 3140–46. http://dx.doi.org/10.1128/jb.01869-06.

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ABSTRACT Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It was previously shown that inserting an adenine residue at position 751 or introducing the U2609C change in 23S rRNA or introducing the K90W replacement in ribosomal protein L22 prevented tryptophan induction of tna operon expression. It was also observed that an adenine a

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20

Shafiekhani, Azizollah, Maryam Zare, and Abdollah Mortezaali. "Tuning the density distribution of deep localized states of TiO 2 nanotube arrays through decoration with Pt and Pt@DLC." Journal of Photochemistry and Photobiology A: Chemistry 380 (May 15, 2019): 1–9. https://doi.org/10.1016/j.jphotochem.2019.111858.

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We demonstrate the possibility to tune the distribution of deep localized states and electronic transport properties of TiO 2 nanotube arrays (TNA)through decoration its surface with Pt or Pt@DLC (Diamond-like carbon). A quantitative calculation of the position of the deep trap states in the band gap and the amount of accumulated charge in deep traps of Pt/TNA and Pt-DLC/TNA electrodes are presented and compared based on photocurrent-voltage curves. Our findings demonstrate that both Pt NPs and Pt@DLC depositions could decrease the quantity of deep localized states in the band gap by saturatin

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21

D'Angio, Richard G., Karen C. Riechers, Robert B. Gilsdorf, and John M. Constantino. "Effect of the Mode of Lipid Administration on Parenteral Nutrition-Related Infections." Annals of Pharmacotherapy 26, no. 1 (1992): 14–17. http://dx.doi.org/10.1177/106002809202600103.

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OBJECTIVE: To determine if total nutrient admixtures (TNAs) influence the rate of infection in clinical practice. DESIGN: Prospective, randomized trial. SETTING: Department of Veterans Affairs Medical Center. PATIENTS: All patients were administered parenteral nutrition (PN) via a central venous catheter and received daily lipids. INTERVENTION: Patients were randomized as to the mode of administration of lipids. Lipids were either administered with other PN components in a TNA or were piggybacked (PB) into the PN solution. MAIN OUTCOME MEASURES: Treatment groups were compared for the rate of o

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22

Bernasconi, Camilla, Giorgio Volponi, Claudia Picozzi, and Roberto Foschino. "Use of the tna Operon as a New Molecular Target for Escherichia coli Detection." Applied and Environmental Microbiology 73, no. 19 (2007): 6321–25. http://dx.doi.org/10.1128/aem.00606-07.

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ABSTRACT A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon.

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23

Yang, Rui, Luis R. Cruz-Vera, and Charles Yanofsky. "23S rRNA Nucleotides in the Peptidyl Transferase Center Are Essential for Tryptophanase Operon Induction." Journal of Bacteriology 191, no. 11 (2009): 3445–50. http://dx.doi.org/10.1128/jb.00096-09.

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ABSTRACT Distinct features of the ribosomal peptide exit tunnel are known to be essential for recognition of specific amino acids of a nascent peptidyl-tRNA. Thus, a tryptophan residue at position 12 of the peptidyl-tRNA TnaC-tRNAPro leads to the creation of a free tryptophan binding site within the ribosome at which bound tryptophan inhibits normal ribosome functions. The ribosomal processes that are inhibited are hydrolysis of TnaC-tRNAPro by release factor 2 and peptidyl transfer of TnaC of TnaC-tRNAPro to puromycin. These events are normally performed in the ribosomal peptidyl transferase

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24

Abdulrahman, Amal, Zaina Algarni, Nejib Ghazouani, Saad Sh Sammen, Abdelfattah Amari, and Miklas Scholz. "Enhancing Photocatalytic Activity with the Substantial Optical Absorption of Bi2S3-SiO2-TiO2/TiO2 Nanotube Arrays for Azo Dye Wastewater Treatment." Water 17, no. 13 (2025): 1875. https://doi.org/10.3390/w17131875.

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One-dimensional TiO2 nanotube arrays (TNAs) were vertically aligned and obtained via the electrochemical anodization method. In this study, Bi2S3-TiO2-SiO2/TNA heterojunction photocatalysts were successfully prepared with different amounts of Bismuth(III) sulfide (Bi2S3) loading on the TNAs by the successive ionic layer adsorption and reaction (SILAR) method and characterized by X-ray diffraction (XRD) patterns, field-emission scanning electron microscope–energy-dispersive spectroscopy (FESEM-EDS), Fourier transform infrared (FTIR) spectra, ultraviolet-visible diffuse reflectance spectra (UV–V

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25

Л.А., КАЛАШНИКОВА, БАГАЛЬ И.Е., МУРУГИНА Н.Е., КАЛАШНИКОВ В.Е. та СЕНИНА Р.Ю. "ПОЛИМОРФИЗМ ГЕНА TNF -824A/GУ КРУПНОГО РОГАТОГО СКОТА МОЛОЧНЫХ ПОРОД". Molochnoe i miasnoe skotovodstvo, № 5 (25 листопада 2024): 8–10. http://dx.doi.org/10.33943/mms.2024.98.85.002.

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Представлены результаты генотипирования крупного рогатого скота по полиморфизму гена фактора некроза опухоли TNF -824A/G. У 5 изученных пород скота разного происхождения (n=159) установлено преобладание аллеля TNFG: частота его встречаемости у коров голштинской породы составила 0,58, холмогорской — 0,61, айрширской — 0,80, симментальской — 0,93 и бурой швицкой — 1,0. Все исследованные животные бурой швицкой породы (n=36) имели гомозиготный генотип TNFGG. Высокая частота встречаемости этого генотипа выявлена у особей симментальской (86%) и айрширской (66%) пород. Большинство коров голштинской (

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26

Gong, Ming, Feng Gong, and Charles Yanofsky. "Overexpression of tnaC of Escherichia coli Inhibits Growth by Depleting tRNA2Pro Availability." Journal of Bacteriology 188, no. 5 (2006): 1892–98. http://dx.doi.org/10.1128/jb.188.5.1892-1898.2006.

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ABSTRACT Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome stalling after translation of tnaC, the coding region for a 24-residue leader peptide. The last sense codon of tnaC, proline codon 24 (CCU), is translated by tRNA2 Pro. We analyzed the consequences of overexpression of tnaC from a multicopy plasmid and observed that under inducing conditions more than 60% of the tRNA2 Pro in the cell was sequestered in ribosomes as TnaC-tRNA2 Pro. The half-life o

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27

Dzmitruk, Volha, Evgeny Apartsin, Aliaksei Ihnatsyeu-Kachan, Viktar Abashkin, Dzmitry Shcharbin, and Maria Bryszewska. "Dendrimers Show Promise for siRNA and microRNA Therapeutics." Pharmaceutics 10, no. 3 (2018): 126. http://dx.doi.org/10.3390/pharmaceutics10030126.

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The lack of an appropriate intracellular delivery system for therapeutic nucleic acids (TNAs) is a major problem in molecular biology, biotechnology, and medicine. A relatively new class of highly symmetrical hyperbranched polymers, called dendrimers, shows promise for transporting small TNAs into both cells and target tissues. Dendrimers have intrinsic advantages for this purpose: their physico-chemical and biological properties can be controlled during synthesis, and they are able to transport large numbers of TNA molecules that can specifically suppress the expression of single or multiple

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28

Oudeman, Eline A., Esther E. Bron, Renske M. Van den Berg-Vos, et al. "Cerebral Perfusion and the Occurrence of Nonfocal Transient Neurological Attacks." Cerebrovascular Diseases 47, no. 5-6 (2019): 303–8. http://dx.doi.org/10.1159/000502334.

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Introduction: Nonfocal transient neurological attacks (TNAs) are associated with an increased risk of cardiac events, stroke and dementia. Their etiology is still unknown. Global cerebral hypoperfusion has been suggested to play a role in their etiology, but this has not been investigated. We assessed whether lower total brain perfusion is associated with a higher occurrence of TNAs. Methods: Between 2015 and 2018, patients with heart failure were included in the Heart Brain Connection study. Patients underwent brain magnetic resonance imaging, including quantitative magnetic resonance angiogr

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29

Liu, Min-Hsien, Cheng Chen, and Yaw-Shun Hong. "Theoretical study of the unimolecular decomposition mechanisms of energetic TNAD and TNAZ explosives." International Journal of Quantum Chemistry 102, no. 4 (2005): 398–408. http://dx.doi.org/10.1002/qua.20284.

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30

Shafiekhani, Azizollah, Maryam Zare, and Abdollah Mortezaali. "Photoelectrochemical determination of shallow and deep trap states of platinum-decorated TiO2 nanotube arrays for photocatalytic applications." Journal of Physical Chemistry C 120, no. 17 (2016): 9017–27. https://doi.org/10.1021/acs.jpcc.5b11987.

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A novel insight into the effect of Pt decoration on electrochemical and photoelectrochemical behavior of TiO2 nanotube arrays (TNA) was developed in this study. TNA samples were prepared via a two-step anodization of a titanium foil and decorated with Pt by a facile photodeposition method. The formation of metallic Pt were confirmed by X-ray photoelectron spectroscopy (XPS). On the basis of our calculations, the localized states and surface states induced by Pt deposition into the bandgap of titania nanotubes, play a dominant role in trapping/detrapping charge carriers and electron transfer to

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31

Oudeman, Eline A., Eline J. Volkers, Jacoba P. Greving, Catharina JM Klijn, Ale Algra, and L. Jaap Kappelle. "Nonfocal transient neurological attacks in patients with carotid artery occlusion." European Stroke Journal 4, no. 1 (2018): 50–54. http://dx.doi.org/10.1177/2396987318818779.

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Introduction Nonfocal transient neurological attacks (TNAs) are episodes with atypical, nonlocalizing cerebral symptoms. We examined the prevalence of nonfocal TNAs, in patients with and without carotid artery occlusion (CAO). Methods We included 67 patients with CAO and 62 patients without CAO. In both groups, patients had a history of transient ischemic attack (TIA) or nondisabling ischemic stroke in the anterior circulation that had occurred >6 months before inclusion. Patients without CAO did not have ipsilateral or contralateral carotid artery stenosis of ≥50%. All patients were interv

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Shafiekhani, Azizollah, Maryam Zare, Shahram Solaymani, Slawomir Kulesza, Ştefan Ţălu, and Miroslaw Bramowicz. "Evolution of rough-surface geometry and crystalline structures of aligned TiO2 nanotubes for photoelectrochemical water splitting." Scientific Reports 8, no. 1 (2018): 1–11. https://doi.org/10.1038/s41598-018-29247-3.

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Nowadays, increasing awareness of environment and fossil fuels protection stimulates intensive research on clean and renewable sources of energy. Production of hydrogen from water through solar-driven splitting reactions is one of the most promising approaches in the field of photoelectrochemistry (PEC). In this work we have fabricated well-aligned, highly-ordered, smooth-mouth TiO2 nanotube arrays (TNAs) in a two-step anodization process of titanium foil, which were then used as photoelectrodes for PEC water splitting. It demonstrates for the first time correspondence between non-linear compo

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Oshchepkova, Anastasiya, Marina Zenkova, and Valentin Vlassov. "Extracellular Vesicles for Therapeutic Nucleic Acid Delivery: Loading Strategies and Challenges." International Journal of Molecular Sciences 24, no. 8 (2023): 7287. http://dx.doi.org/10.3390/ijms24087287.

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Extracellular vesicles (EVs) are membrane vesicles released into the extracellular milieu by cells of various origins. They contain different biological cargoes, protecting them from degradation by environmental factors. There is an opinion that EVs have a number of advantages over synthetic carriers, creating new opportunities for drug delivery. In this review, we discuss the ability of EVs to function as carriers for therapeutic nucleic acids (tNAs), challenges associated with the use of such carriers in vivo, and various strategies for tNA loading into EVs.

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Łętocha, Aneta, Daniel Toboła, and Tatiana Miller. "Surface topography analysis of Vanadis 6 tool steel after selected sequential surface treatment processes." Mechanik 91, no. 12 (2018): 1124–28. http://dx.doi.org/10.17814/mechanik.2018.12.200.

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Issues related with an influence of selected sequential surface treatment processes on the stereometric features of the formed surface layer (SL) of Vanadis 6 steel are presented. Some variants of machining include: turning (T), turning–burnishing (TN), turning–burnishing–nitriding (TNA), turning–burnishing–sulfonitriding (TNAS) and turning–burnishing–PVD coating (TNPVD). Studies were carried out by contact profiling method with use TOPO 01P device – produced by The Institute of Advanced Manufacturing Technology. Qualitative changes in the graphs of examined surfaces and also changes in the re

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Bai, Long, Peiru Chen, Bin Tang, Ruiqiang Hang, and Yin Xiao. "Correlation between LncRNA Profiles in the Blood Clot Formed on Nano-Scaled Implant Surfaces and Osseointegration." Nanomaterials 11, no. 3 (2021): 674. http://dx.doi.org/10.3390/nano11030674.

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Implant surfaces with a nanoscaled pattern can dominate the blood coagulation process resulting in a defined clot structure and its degradation behavior, which in turn influence cellular response and the early phase of osseointegration. Long non-coding (Lnc) RNAs are known to regulate many biological processes in the skeletal system; however, the link between the LncRNA derived from the cells within the clot and osseointegration has not been investigated to date. Hence, the sequence analysis of LncRNAs expressed within the clot formed on titania nanotube arrays (TNAs) with distinct nano-scaled

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Gilbert, Francis, Jacques E. Leclerc, Marianne Deschênes, Anne-Sophie Julien, and Iseult Grenier-Ouellette. "Furlow Palatoplasty, Nasopharyngeal Size, and Sleep Oximetry." Cleft Palate-Craniofacial Journal 57, no. 7 (2020): 819–27. http://dx.doi.org/10.1177/1055665619900865.

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Objectives: (1) To assess the evolution of prepalatoplasty sleep oximetry (PRESO) and postpalatoplasty sleep oximetry (POSSO) in cleft patients and (2) to evaluate the impact of the size of the nasopharynx on PRESO and POSSO values. Study Design: Retrospective cohort study. Patients and Methods: In 81 patients with cleft palate and/or cleft lip, the following data were prospectively collected: patient demographics and prepalatoplasty cleft palate measurements. All the patients had at least 1 PRESO and POSSO. A Kaplan-Meier curve was obtained from all the sleep oximetry results. Transverse naso

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Konan, K. V., and C. Yanofsky. "Regulation of the Escherichia coli tna operon: nascent leader peptide control at the tnaC stop codon." Journal of bacteriology 179, no. 5 (1997): 1774–79. http://dx.doi.org/10.1128/jb.179.5.1774-1779.1997.

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Lu, Hang, Jason Catania, Katalin Baranji, et al. "Characterization of the Native Form of Anthrax Lethal Factor for Use in the Toxin Neutralization Assay." Clinical and Vaccine Immunology 20, no. 7 (2013): 986–97. http://dx.doi.org/10.1128/cvi.00046-13.

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ABSTRACTThe cell-based anthrax toxin neutralization assay (TNA) is used to determine functional antibody titers of sera from animals and humans immunized with anthrax vaccines. The anthrax lethal toxin is a critical reagent of the TNA composed of protective antigen (PA) and lethal factor (LF), which are neutralization targets of serum antibodies. Cytotoxic potency of recombinant LF (rLF) lots can vary substantially, causing a challenge in producing a renewable supply of this reagent for validated TNAs. To address this issue, we characterized a more potent rLF variant (rLF-A) with the exact nat

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Kwon, Young-Man, and Bernard Weiss. "Production of 3-Nitrosoindole Derivatives by Escherichia coli during Anaerobic Growth." Journal of Bacteriology 191, no. 17 (2009): 5369–76. http://dx.doi.org/10.1128/jb.00586-09.

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ABSTRACT When Escherichia coli K-12 is grown anaerobically in medium containing tryptophan and sodium nitrate, it produces red compounds. The reaction requires functional genes for trytophanase (tnaA), a tryptophan permease (tnaB), and a nitrate reductase (narG), as well as a natural drop in the pH of the culture. Mass spectrometry revealed that the purified chromophores had mass/charge ratios that closely match those for indole red, indoxyl red, and an indole trimer. These compounds are known products of chemical reactions between indole and nitrous acid. They are derived from an initial reac

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Firth, Dawn H. "The trainee nursing associates' experience of academic learning: the first 6 months." British Journal of Healthcare Assistants 17, no. 10 (2023): 362–70. http://dx.doi.org/10.12968/bjha.2023.17.10.362.

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Background: The introduction of the nursing associate role has aimed to provide a different route into nursing careers. Education standards have been structured in line with Nursing and Midwifery Council (NMC) standards for nursing associates for them to meet the proficiency requirement to enter the NMC register ( NMC, 2018c ). However, there are pedagogic challenges due to the inclusive nature of recruitment, which requires only baseline entry qualifications. Objectives: The purpose of this research is to investigate the experience that trainee nursing associates (TNAs) have of academic learn

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Kamath, A. V., and C. Yanofsky. "Roles of the tnaC-tnaA spacer region and Rho factor in regulating expression of the tryptophanase operon of Proteus vulgaris." Journal of bacteriology 179, no. 5 (1997): 1780–86. http://dx.doi.org/10.1128/jb.179.5.1780-1786.1997.

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Wang, Dandan, Xuedong Ding, and Philip N. Rather. "Indole Can Act as an Extracellular Signal inEscherichia coli." Journal of Bacteriology 183, no. 14 (2001): 4210–16. http://dx.doi.org/10.1128/jb.183.14.4210-4216.2001.

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ABSTRACT Previous work has shown that lacZ fusions to thecysK, astD, tnaB, and gabT genes inEscherichia coli are activated by self-produced extracellular signals. Using a combination of ethyl acetate extraction, reversed-phase C18 chromatography, and thin-layer chromatography, we have purified an extracellular activating signal from E. coli supernatants. Mass spectrometry revealed a molecule with an m/z peak of 117, consistent with indole. Nuclear magnetic resonance analysis of the purified E. colifactor and synthetic indole revealed identical profiles. Using synthetic indole, a dose-dependent

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Costa, Sergio Olavo Pinto da. "Gene tnaA." Genética na Escola 8, no. 1 (2013): 104–9. http://dx.doi.org/10.55838/1980-3540.ge.2013.152.

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Bactérias com alto nível de resistência a um dado antibiótico podem, curiosamente, ajudar altruisticamente bactérias vizinhas, menos resistentes a esse antibiótico, graças à produção de indol que leva estas bactérias menos resistentes a expulsar o antibiótico de seu interior.

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Martin, Kimberly, Gregory Morlin, Arnold Smith, Andrea Nordyke, Abraham Eisenstark, and Miriam Golomb. "The Tryptophanase Gene Cluster of Haemophilus influenzae Type b: Evidence for Horizontal Gene Transfer." Journal of Bacteriology 180, no. 1 (1998): 107–18. http://dx.doi.org/10.1128/jb.180.1.107-118.1998.

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ABSTRACT Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, including meningitis. As a first step in investigating the relationship between tryptophan catabolism and virulence, we have identified genes in pathogenic H. influenzae which are homologous to the tryptophanase (tna) operon of Escherichia coli. The tna genes are located o

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Do, Tho Chau Minh Vinh, Duy Quoc Nguyen, Kien Trung Nguyen, and Phuoc Huu Le. "TiO2 and Au-TiO2 Nanomaterials for Rapid Photocatalytic Degradation of Antibiotic Residues in Aquaculture Wastewater." Materials 12, no. 15 (2019): 2434. http://dx.doi.org/10.3390/ma12152434.

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Antibiotic residues in aquaculture wastewater are considered as an emerging environmental problem, as they are not efficiently removed in wastewater treatment plants. To address this issue, we fabricated TiO2 nanotube arrays (TNAs), TiO2 nanowires on nanotube arrays (TNWs/TNAs), Au nanoparticle (NP)-decorated-TNAs, and TNWs/TNAs, which were applied for assessing the photocatalytic degradation of eight antibiotics, simultaneously. The TNAs and TNWs/TNAs were synthesized by anodization using an aqueous NH4F/ethylene glycol solution. Au NPs were synthesized by chemical reduction method, and used

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Nguyen, Phuong Hoang, Tho Truong Nguyen, Thi Minh Cao, Hien Duy Tong, and Viet Van Pham. "Photoelectrochemical water oxidation over TiO₂ nanotubes modified with MoS₂ and g-C₃N₄." Beilstein Journal of Nanotechnology 13 (December 16, 2022): 1541–50. http://dx.doi.org/10.3762/bjnano.13.127.

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TiO2 nanotube arrays (TNAs) have been studied for photoelectrochemical (PEC) water splitting. However, there are two major barriers of TNAs, including a low photo-response and the fast charge carrier recombination in TNAs, leading to poor photocatalytic efficiency. Through a comparison of MoS2/TNAs and g-C3N4/TNAs, it was found that TNAs modified with MoS2 and g-C3N4 exhibited a current density of, respectively, 210.6 and 139.6 μA·cm−2 at an overpotential of 1.23 V vs RHE, which is 18.2 and 12 times higher than that of pure TNAs under the same conditions. The stability of the MoS2/TNAs heteroj

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Lazen, Philipp, Pedro Lima Cardoso, Sukrit Sharma, et al. "A Comparison of 7 Tesla MR Spectroscopic Imaging and 3 Tesla MR Fingerprinting for Tumor Localization in Glioma Patients." Cancers 16, no. 5 (2024): 943. http://dx.doi.org/10.3390/cancers16050943.

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This paper investigated the correlation between magnetic resonance spectroscopic imaging (MRSI) and magnetic resonance fingerprinting (MRF) in glioma patients by comparing neuro-oncological markers obtained from MRSI to T1/T2 maps from MRF. Data from 12 consenting patients with gliomas were analyzed by defining hotspots for T1, T2, and various metabolic ratios, and comparing them using Sørensen–Dice similarity coefficients (DSCs) and the distances between their centers of intensity (COIDs). The median DSCs between MRF and the tumor segmentation were 0.73 (T1) and 0.79 (T2). The DSCs between MR

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Zhang, Zhi, Hwa Kyung Nam, Spencer Crouch, and Nan E. Hatch. "Tissue Nonspecific Alkaline Phosphatase Function in Bone and Muscle Progenitor Cells: Control of Mitochondrial Respiration and ATP Production." International Journal of Molecular Sciences 22, no. 3 (2021): 1140. http://dx.doi.org/10.3390/ijms22031140.

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Tissue nonspecific alkaline phosphatase (TNAP/Alpl) is associated with cell stemness; however, the function of TNAP in mesenchymal progenitor cells remains largely unknown. In this study, we aimed to establish an essential role for TNAP in bone and muscle progenitor cells. We investigated the impact of TNAP deficiency on bone formation, mineralization, and differentiation of bone marrow stromal cells. We also pursued studies of proliferation, mitochondrial function and ATP levels in TNAP deficient bone and muscle progenitor cells. We find that TNAP deficiency decreases trabecular bone volume f

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Johnson, K. A., L. Hessle, S. Vaingankar, et al. "Osteoblast tissue-nonspecific alkaline phosphatase antagonizes and regulates PC-1." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 279, no. 4 (2000): R1365—R1377. http://dx.doi.org/10.1152/ajpregu.2000.279.4.r1365.

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Tissue-nonspecific alkaline phosphatase (TNAP) is essential for bone matrix mineralization, but the central mechanism for TNAP action remains undefined. We observed that ATP-dependent 45Ca precipitation was decreased in calvarial osteoblast matrix vesicle (MV) fractions from TNAP−/− mice, a model of infantile hypophosphatasia. Because TNAP hydrolyzes the mineralization inhibitor inorganic pyrophosphate (PPi), we assessed phosphodiesterase nucleotide pyrophosphatase (PDNP/NTPPPH) activity, which hydrolyzes ATP to generate PPi. Plasma cell membrane glycoprotein-1 (PC-1), but not the isozyme B10

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Chen, Chia-Hung, Yen-Ping Peng, Ming-Hsun Lin, Ken-Lin Chang, Yung-Chang Lin, and Jian Sun. "Iron Modified Titanate Nanotube Arrays for Photoelectrochemical Removal of E. coli." Nanomaterials 11, no. 8 (2021): 1944. http://dx.doi.org/10.3390/nano11081944.

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This study used iron modified titanate nanotube arrays (Fe/TNAs) to remove E. coli in a photoelectrochemical system. The Fe/TNAs was synthesized by the anodization method and followed by the square wave voltammetry electrochemical deposition (SWVE) method with ferric nitrate as the precursor. Fe/TNAs were characterized by SEM, XRD, XPS, and UV-vis DRS to investigate the surface properties and light absorption. As a result, the iron nanoparticles (NPs) were successfully deposited on the tubular structure of the TNAs, which showed the best light utilization. Moreover, the photoelectrochemical (P

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