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Relevant bibliographies by topics / 16S rDNA gene sequencing

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Academic literature on the topic '16S rDNA gene sequencing'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "16S rDNA gene sequencing"

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Reischl, U., K. Feldmann, L. Naumann, et al. "16S rRNA Sequence Diversity in Mycobacterium celatum Strains Caused by Presence of Two Different Copies of 16S rRNA Gene." Journal of Clinical Microbiology 36, no. 6 (1998): 1761–64. http://dx.doi.org/10.1128/jcm.36.6.1761-1764.1998.

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Direct sequencing of the 16S rRNA gene (16S rDNA) ofMycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.

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Wang, Haiyin, Pengcheng Du, Juan Li, et al. "Comparative analysis of microbiome between accurately identified 16S rDNA and quantified bacteria in simulated samples." Journal of Medical Microbiology 63, no. 3 (2014): 433–40. http://dx.doi.org/10.1099/jmm.0.060616-0.

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Although 16S rRNA gene (rDNA) sequencing is the gold standard for categorizing bacteria or characterizing microbial communities its clinical utility is limited by bias in metagenomic studies, in either the experiments or the data analyses. To evaluate the efficiency of current metagenomic methods, we sequenced seven simulated samples of ten bacterial species mixed at different concentrations. The V3 region of 16S rDNA was targeted and used to determine the distribution of bacterial species. The number of target sequences in individual simulated samples was in the range 1–1000 to provide a bett

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Chatellier, Sonia, Nathalie Mugnier, Françoise Allard, et al. "Comparison of two approaches for the classification of 16S rRNA gene sequences." Journal of Medical Microbiology 63, no. 10 (2014): 1311–15. http://dx.doi.org/10.1099/jmm.0.074377-0.

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The use of 16S rRNA gene sequences for microbial identification in clinical microbiology is accepted widely, and requires databases and algorithms. We compared a new research database containing curated 16S rRNA gene sequences in combination with the lca (lowest common ancestor) algorithm (RDB-LCA) to a commercially available 16S rDNA Centroid approach. We used 1025 bacterial isolates characterized by biochemistry, matrix-assisted laser desorption/ionization time-of-flight MS and 16S rDNA sequencing. Nearly 80 % of isolates were identified unambiguously at the species level by both classificat

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Korczak, Bożena, Henrik Christensen, Stefan Emler, Joachim Frey, and Peter Kuhnert. "Phylogeny of the family Pasteurellaceae based on rpoB sequences." International Journal of Systematic and Evolutionary Microbiology 54, no. 4 (2004): 1393–99. http://dx.doi.org/10.1099/ijs.0.03043-0.

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Sequences of the gene encoding the β-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained wit

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Lawton, Samantha J., Allison M. Weis, Barbara A. Byrne, et al. "Comparative analysis of Campylobacter isolates from wild birds and chickens using MALDI-TOF MS, biochemical testing, and DNA sequencing." Journal of Veterinary Diagnostic Investigation 30, no. 3 (2018): 354–61. http://dx.doi.org/10.1177/1040638718762562.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1). The test results for all 27 isolates were in 100% agreement between MALDI

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Li, Yong Feng, Yi Xuan Wang, Lu Wang, and Zhan Qing Wang. "Sequence Length Variation of Internal Genic Space of 16S rDNA-23S rDNA in Bacterium with High Yield of Hydrogen Production." Advanced Materials Research 183-185 (January 2011): 1413–16. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.1413.

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To develop the identification of species for fermentative biohydrogen-producing bacterium, scholars have found a method which is based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions. In the study, a large fragment of the rDNA operon, including the 16S rDNA, the intergenic spacer region (ISR) and approximately 2000 bases of the 23S rDNA, were polymerasechain reaction (PCR) amplified. The PCR amplification of the genomic DNA of Leptonema ilk strain 3055 using primers directed against conserved regions of the rRNA operon provided evidence that the 16S and 23S rRNA ge

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Carvalho-Netto, Osmar Vaz de, Daniel Dias Rosa, and Luis Eduardo Aranha Camargo. "Identificarion of contaminant bacteria in cachaça yeast by 16s rDNA gene sequencing." Scientia Agricola 65, no. 5 (2008): 508–15. http://dx.doi.org/10.1590/s0103-90162008000500010.

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Cachaça is a typical Brazilian liquor produced from the distillation of fermented sugarcane juice mainly by Saccharomyces cerevisiae. Most of the domestic production is artisanal, and producers usually are not concerned regarding microbiological control of the fermentation. This study aimed to characterize the contaminant bacterial community of the yeast used in the production of cachaça in an artisanal still. Four samples were collected, of which one (NA) was used for comparison purposes and was collected one year earlier. The remaining samples were collected at three different periods: at th

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Agudelo-Pérez, Sergio, A. Melissa Moreno, Juliana Martínez-Garro, et al. "16S rDNA Sequencing for Bacterial Identification in Preterm Infants with Suspected Early-Onset Neonatal Sepsis." Tropical Medicine and Infectious Disease 9, no. 7 (2024): 152. http://dx.doi.org/10.3390/tropicalmed9070152.

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Background: The high prevalence of suspected early-onset neonatal sepsis among preterm infants leads to immediate antibiotic administration upon admission. Notably, most blood cultures for suspected early-onset neonatal sepsis do not yield a causative pathogen. This study aimed to assess polymerase chain reaction (PCR) targeting the variable region V4 of the 16S ribosomal gene (16S rDNA) and Sanger sequencing for bacterial identification in preterm infants with suspected early-onset neonatal sepsis. Methods: Therefore, this prospective study was conducted. Preterm infants with suspected early-

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Hinrikson, Hans Peter, Fabrizio Dutly, and Martin Altwegg. "Evaluation of a Specific Nested PCR Targeting Domain III of the 23S rRNA Gene of “Tropheryma whippelii” and Proposal of a Classification System for Its Molecular Variants." Journal of Clinical Microbiology 38, no. 2 (2000): 595–99. http://dx.doi.org/10.1128/jcm.38.2.595-599.2000.

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“Tropheryma whippelii”-associated infections are usually confirmed histopathologically by using light microscopy. PCR assays targeting the 16S rRNA gene (16S rDNA) of “T. whippelii” are increasingly being applied for this purpose. Compared to microscopic analysis, PCR seems to be more sensitive, as indicated by the fact that several cases of Whipple's disease with negative histopathological findings but positive PCR results have been reported. Considering the lack of pathognomonic clinical features for this disease and the fact that “T. whippelii” DNA has repeatedly been found in patients with

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da Silva, Cleiziane Bispo, Hellen Ribeiro Martins dos Santos, Phellippe Arthur Santos Marbach, et al. "First-tier detection of intragenomic 16S rRNA gene variation in culturable endophytic bacteria from cacao seeds." PeerJ 7 (November 20, 2019): e7452. http://dx.doi.org/10.7717/peerj.7452.

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Background Intragenomic variability in 16S rDNA is a limiting factor for taxonomic and diversity characterization of Bacteria, and studies on its occurrence in natural/environmental populations are scarce. In this work, direct DNA amplicon sequencing coupled with frequent-cutter restriction analysis allowed detection of intragenomic 16S rDNA variation in culturable endophytic bacteria from cacao seeds in a fast and attractive manner. Methods Total genomic DNA from 65 bacterial strains was extracted and the 16S rDNA hyper variable V5–V9 regions were amplified for enzyme digestion and direct San

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Dissertations / Theses on the topic "16S rDNA gene sequencing"

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Ziegler, Katie. "Phylogenetic Analysis of a Group of Enteric Bacteria Based on 16S rDNA Gene Sequencing." Miami University Honors Theses / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111684418.

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Torres, Adalgisa Ribeiro. "Diversidade genética de enterobactérias endofíticas de diferentes hospedeiros e colonização de Catharantus roseus por endófitos expressando o gene gfp." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-03062005-152149/.

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Bactérias endofíticas são aquelas que habitam o interior de tecidos vegetais, sem causar dano aparente aos mesmos, além de desempenhar funções importantes no processo de adaptação das plantas. Especial interesse tem sido dado a tais bactérias devido ao seu potencial no controle biológico. Por isso, é muito importante estudar a diversidade genética de endófitos, além de avaliar o impacto da introdução de endófitos geneticamente modificados no ambiente. Estudos vêm sendo feitos nesse sentido, mas não com bactérias endofíticas da família Enterobacteriaceae. Desta forma, o presente trabalho tev

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Fogler, Kendall Wilson. "Effect of Soil Amendments from Antibiotic Treated Cows on Antibiotic Resistant Bacteria and Genes Recovered from the Surfaces of Lettuce and Radishes: Field Study." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/92587.

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Cattle are commonly treated with antibiotics that may survive digestion and promote antibiotic resistance when manure or composted manure is used as a soil amendment for crop production. This study was conducted to determine the effects of antibiotic administration and soil amendment practices on microbial diversity and antibiotic resistance of bacteria recovered from the surfaces of lettuce and radishes grown using recommended application rates. Vegetables were planted in field plots amended with raw manure from antibiotic-treated dairy cows, composted-manure from cows with different historie

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ARAÚJO, Livia Caroline Alexandre de. "Caracterização molecular das linhagens de Zymomonas mobilis da coleção de micro-organismos UFPEDA." Universidade Federal de Pernambuco, 2014. https://repositorio.ufpe.br/handle/123456789/17179.

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Tam, Man-wah. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/hkuto/record/B31970783.

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Li, Kwan-hing. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31971982.

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Li, Kwan-hing, and 李群卿. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31971982.

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譚文華 and Man-wah Tam. "Identification of bacterial pathogens by 16S ribosomal RNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31970783.

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Netto, Osmar Vaz de Carvalho. "Identificação de bactérias contaminantes de fermento de cachaça por seqüenciamento do gene 16S rDNA." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08082007-164157/.

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A cachaça é uma bebida típica brasileira produzida a partir da destilação do caldo de canade- açúcar fermentado principalmente por Saccharomyces cerevisiae. Grande parte da produção nacional é artesanal, e não há uma preocupação por parte dos produtores quanto ao controle microbiológico da fermentação. Este trabalho objetivou caracterizar a comunidade bacteriana contaminante do fermento utilizado na produção de cachaça. Foram coletadas quatro amostras em um alambique artesanal. A primeira (NA) foi coletada um ano anterior às outras três e utilizada como controle. As restantes foram coletadas a

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鄧莉莉 and Lee-lee Jade Teng. "Identification of thermo-tolerant campylobacter fetus by 16S ribosomalRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B3196994X.

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Books on the topic "16S rDNA gene sequencing"

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Goldenberger, Daniel. Detection of bacterial pathogens in clinical specimens by broad-range PCR amplification and direct sequencing of part of the 16S rRNA gene. 1997.

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Kirchman, David L. Community structure of microbes in natural environments. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198789406.003.0004.

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Community structure refers to the taxonomic types of microbes and their relative abundance in an environment. This chapter focuses on bacteria with a few words about fungi; protists and viruses are discussed in Chapters 9 and 10. Traditional methods for identifying microbes rely on biochemical testing of phenotype observable in the laboratory. Even for cultivated microbes and larger organisms, the traditional, phenotype approach has been replaced by comparing sequences of specific genes, those for 16S rRNA (archaea and bacteria) or 18S rRNA (microbial eukaryotes). Cultivation-independent appro

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Book chapters on the topic "16S rDNA gene sequencing"

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Einarsson, Gisli G., and Sébastien Boutin. "Techniques: culture, identification and 16S rRNA gene sequencing." In The Lung Microbiome. European Respiratory Society, 2019. http://dx.doi.org/10.1183/2312508x.10000819.

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Pimenta, Mélanie, Margaux Gaschet, and Sylvain Meyer. "Targeted 16S rRNA Gene Sequencing for Water Samples." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4726-4_6.

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Fantini, Elio, Giulio Gianese, Giovanni Giuliano, and Alessia Fiore. "Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-1720-4_5.

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James, Greg. "Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene." In PCR for Clinical Microbiology. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9039-3_28.

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Oskay, Mustafa. "Optimization of Colony Polymerase Chain Reaction for the Identification of Actinobacteria by Sequencing of the 16S rDNA." In Protocols of Actinomycetes. CRC Press, 2024. http://dx.doi.org/10.1201/9781003398400-36.

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Formenti, Fabio, Gabriel Rinaldi, Cinzia Cantacessi, and Alba Cortés. "Helminth Microbiota Profiling Using Bacterial 16S rRNA Gene Amplicon Sequencing: From Sampling to Sequence Data Mining." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1681-9_15.

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Matsuo, Yoshiyuki. "Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2996-3_14.

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Naqib, Ankur, Silvana Poggi, Weihua Wang, Marieta Hyde, Kevin Kunstman, and Stefan J. Green. "Making and Sequencing Heavily Multiplexed, High-Throughput 16S Ribosomal RNA Gene Amplicon Libraries Using a Flexible, Two-Stage PCR Protocol." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7834-2_7.

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Urumbil, Sithara K. "METAGENOMICS THROUGH NEXT GENERATION SEQUENCING- A LEAP IN THE EXPLORATION OF THE HIDDEN WORLD OF ENDOPHYTIC MICROBIAL COMMUNITY." In Recent Trends in Endophyte Research. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/nbennurch265.

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Endophytes are microbes residing in plant tissue and they are clandestine masters of plant growth regulation. Quantifying taxon frequency and community membership of this microflora will allow us to assess their potential at unprecedented depth. The generation of highly accurate genomic data with advanced computational tools points to application-based perspectives of these phytomicrobes. Whole metagenome analysis and metagenome analysis of 16S rDNA sequences are indispensable technologies to explore this valuable resource of microorganisms. Applications of metagenomic analysis in the field of

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Shah, Neethu, Haixu Tang, Thomas G. Doak, and Yuzhen Ye. "COMPARING BACTERIAL COMMUNITIES INFERRED FROM 16S rRNA GENE SEQUENCING AND SHOTGUN METAGENOMICS." In Biocomputing 2011. WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814335058_0018.

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Conference papers on the topic "16S rDNA gene sequencing"

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Le Borgne, S., J. Jan, J. M. Romero, and M. Amaya. "Impact of Molecular Biology Techniques on the Detection and Characterization of Microorganisms and Biofilms Involved in MIC." In CORROSION 2002. NACE International, 2002. https://doi.org/10.5006/c2002-02461.

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Abstract Recently, the application of molecular techniques has greatly improved our knowledge of microbial diversity and distribution in environmental samples. In this paper, we have reviewed the molecular biology techniques that can be used to study microorganisms and biofilms involved in the MIC phenomenon. These techniques include 16S ribosomal DNA (16S rDNA) gene based techniques. In particular, 16S rDNA sequencing, the construction of 16S rDNAs libraries, Fluorescence In Situ Hybridization (FISH), Denaturing Gradient Gel Electrophoresis (DGGE) and Random Amplification of Polymorphic DNA (

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Bartling, Craig, Angela Minard-Smith, Kate H. Kucharzyk, Jennifer Busch-Harris, and Larry Mullins. "Interpreting Omic Data for Microbially Influenced Corrosion: Lessons from a Case Study Involving a Seawater Injection System." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09445.

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Abstract Microbiologically influenced corrosion (MIC) is a significant source of pitting corrosion affecting oil and gas pipelines, wells, and a variety of surface facilities. Understanding of MIC is greatly enhanced through DNA and protein sequencing technologies. This paper highlights the need to understand the methods used to generate the data, the data quality, and the limitations associated with data interpretation through a case study involving the metagenomics and proteomic analysis of pig envelope debris and seawater samples from various locations within a seawater injection system sus

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De Paula, Renato, Cruz St Peter, Ian Alex Richardson, et al. "DNA Sequencing of Oilfield Samples: Impact of Protocol Choices on the Microbiological Conclusions." In CORROSION 2018. NACE International, 2018. https://doi.org/10.5006/c2018-11662.

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Abstract In the last decade, molecular microbiology techniques have significantly expanded the understanding of the resident microflora in hydrocarbon reservoirs and production systems. These methods have been steadily accepted by the industry and are widely viewed as accurate, comprehensive and highly valuable tools that augment or may eventually replace conventional methods. The resulting information has helped operators and service companies to develop better monitoring programs, assess risks and tailor mitigation strategies to control undesired microbial activities in wells, flowlines and

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Le Borgne, S., J. Jan, J. L. Garcia Villalobos, M. Amaya, and J. M. Romero. "Initial Developments of a Database of Bacteria Involved in Microbiologically Influenced Corrosion." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04058.

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Abstract An initial database of bacteria involved in MIC is proposed. It is composed of bacteria detected by Molecular Biology techniques through the sequencing of their 16S rDNA genes in bio films, consortia and samples collected in sites potentially affected by MIC problems. The bacteria were not isolated and their participation in MIC should be evaluated. The use of such data is discussed and future directions are also proposed to continue this work.

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Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor

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Romero, J. M., E. Velázquez, J. L. García Villalobos, M. Amaya, and S. Le Borgne. "Genetic Monitoring of Bacterial Populations in a Seawater Injection System. Identification of Biocide Resistant Bacteria and Study of Their Corrosive Effect." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05483.

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Abstract DNA was extracted from a water sample taken from an offshore seawater injection system. DNA was also extracted from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the Polymerase Chain Reaction (PCR) and bacterial diversity was studied using Denaturing Gel Gradient Electrophoresis (DGGE). The obtained results showed that microbial evaluation was biased by the use of artificial culture media although recommended media were used, indicating that microbiological analysis of waters in industrial systems by culturing methods may n

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Demeter, Marc, Shawna Johnston, Kim Dockens, and Raymond J. Turner. "Molecular MIC Diagnoses from ATP Field Test: Streamlined Workflow from Field to 16S rRNA Gene Metagenomics Results." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09420.

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Abstract Microbiologically influenced corrosion (MIC) describes the negative impacts of microbial growth within various industries. While culture based growth tests are the traditional means of assessing MIC, they are not accurate. New culture-independent assays have been developed; one of which is the adenosine triphosphate (ATP) assay. This field assay allows for indirect enumeration of microorganisms; however, it does not divulge which microorganisms are present. Molecular methods such as 16S metagenomics (16S rRNA gene sequencing and data interpretation) are often used for characterizing t

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Manna, Kathleen, Joseph Moore, Kenneth Wunch, et al. "Relative Performance of Various 16S iTag Amplicon Sequencing Primer Pairs in Profiling Microbial Communities Relevant to Oil & Gas Operations." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-13442.

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Abstract The oil and gas industry's efforts to characterize microbial communities in oilfield process fluids has shed increasing light on the influence of bacteria and archaea on hydrocarbon production. Notably, topside or downhole water contamination by microorganisms such as sulfate-reducing bacteria, acid-producing bacteria, and/or other halophiles can negatively impact asset integrity and reduce the quality and quantity of produced hydrocarbons. Molecular microbiology methods have contributed to our understanding of these complex processes by providing unprecedented resolution of resident

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Almahamedh, Hussain H., Charles Williamson, John R. Spear, Brajendra Mishra, and David L. Olson. "Identification of Microorganisms and Their Effects on Corrosion of Carbon Steels Pipelines." In CORROSION 2011. NACE International, 2011. https://doi.org/10.5006/c2011-11231.

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Abstract Microbial influenced corrosion on different grades of carbon steel coupons used in pipelines were investigated in this study. Microbes were isolated and cultivated from oil well produced water samples, and immersion tests were conducted with these waters, microbes and coupons. Bacteria were identified with 16S rRNA gene sequencing. Sequencing analyses revealed the existence of different kinds of bacteria including iron, manganese, and sulfate-reducing bacteria. Corrosion products were characterized by X-ray diffraction (XRD), and scanning electron microscopy (SEM) coupled with energy

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McCulloch, Iain, Michael Whitman, Danika Nicoletti, Greg Howard, and Neil Sharma. "Validation of an Optimized qPCR Workflow for MIC Risk Identification and Oilfield Microbial Monitoring." In MECC 2023. AMPP, 2023. https://doi.org/10.5006/mecc2023-20130.

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In this work, a scalable workflow for field sample preservation, DNA extraction, and quantitative polymerase chain reaction (qPCR) was developed and validated for accurate and rapid oilfield microbial monitoring and microbiologically influenced corrosion (MIC) risk identification. Validation experiments were performed on a variety of challenging oilfield sample types including produced water and pigging sludge to assess the complete optimized qPCR workflow and eight MIC-related qPCR targets including sulfate reducing prokaryotes (SRP) and corrosive methanogens (micH). The predicted in silico t

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Reports on the topic "16S rDNA gene sequencing"

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วิเวกแว่ว, อัมพร, та วิเชฏฐ์ คนซื่อ. ความหลากหลายทางพันธุกรรมของอึ่งบางชนิดในสกุล Microhyla ในพื้นที่สวนสัตว์เปิดเขาเขียว จังหวัดชลบุรี โดยวิเคราะห์จากลำดับนิวคลีโอไทด์ ของยีน COI และยีน 16S rRNA ในไมโทคอนเดรียลดีเอ็นเอ. จุฬาลงกรณ์มหาวิทยาลัย, 2014. https://doi.org/10.58837/chula.res.2014.55.

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การผสมข้ามสายพันธุ์ (hybridization) เป็นการผสมพันธุ์ระหว่างสิ่งมีชีวิตที่มีความใกล้ชิดกันเชิงวิวัฒนาการที่มีโครงสร้างทางพันธุกรรมของประชากรหรือสปีชีส์ที่แตกต่างกัน ทั้งนี้ลูกผสม (hybrids) ที่เกิดขึ้นอาจทำให้เกิดการถ่ายเทเคลื่อนย้ายของยีน (gene flow) ระหว่างประชากรหรือสปีชีส์ได้หากลูกผสมดังกล่าวสามารถอยู่รอดและสืบพันธุ์ได้ ซึ่งสามารถตรวจสอบได้โดยใช้เทคนิคทางด้านอณูชีววิทยา อึ่งน้ำเต้า (Microhyla fissipes) อึ่งข้างดำ (M. heymonsi) และอึ่งลายเลอะ (M. butleri) จัดเป็นสัตว์สะเทินน้ำสะเทินบกที่อยู่ในสกุลเดียวกัน มีขนาดลำตัวใกล้เคียงกันและมีการกระจายอยู่ในทุกภาคของประเทศไทย จากการสำรวจเบื้องต้นพบว่าอ

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Thurston, Alison, Karen Foley, Shelby Rosten, Susan Taylor, Robert Haehnel, and Robyn Barbato. Microbial activity in dust-contaminated Antarctic snow. Engineer Research and Development Center (U.S.), 2023. http://dx.doi.org/10.21079/11681/47681.

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During weather events, particles can accumulate on the snow near the Pegasus ice and Phoenix compacted-snow Runways at the US McMurdo Station in Antarctica. The deposited particles melt into the surface, initially forming steep-sided holes, which can widen into patches of weak and rotten snow and ice. These changes negatively impact the ice and snow runways and snow roads trafficked by vehicles. To understand the importance of microbes on this process, we examined deposited dust particles and their microbial communities in snow samples collected near the runways. Snow samples were analyzed at

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VanderGheynst, Jean, Michael Raviv, Jim Stapleton, and Dror Minz. Effect of Combined Solarization and in Solum Compost Decomposition on Soil Health. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7594388.bard.

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In soil solarization, moist soil is covered with a transparent plastic film, resulting in passive solar heating which inactivates soil-borne pathogen/weed propagules. Although solarization is an effective alternative to soil fumigation and chemical pesticide application, it is not widely used due to its long duration, which coincides with the growing season of some crops, thereby causing a loss of income. The basis of this project was that solarization of amended soil would be utilized more widely if growers could adopt the practice without losing production. In this research we examined three

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