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Dissertations / Theses on the topic '2-dimensional electrophoresis'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Yao, Mingyi. "Proteomic studies of Pseudomonas putida KT2440 in carcinogenicity screening via 2-dimensional gel electrophoresis /." Online version of thesis, 2005. https://ritdml.rit.edu/dspace/handle/1850/1118.

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Misztal, David Richard Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Identification of cellular changes associated with increased production of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41326.

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A proteomics approach was used to identify proteins potentially implicated in the cellular response concurrent with elevated production levels of human follicle stimulating hormone in a recombinant Chinese hamster ovary cell line (Darren cells), using zinc and sodium butyrate in the production media to increase expression. To this end, 2-dimensional gel electrophoresis (2-DGE) was utilized. Firstly, several aspects of 2-DGE were developed for this investigation. Gel drying conditions were optimized, and a glycine-free blotting method is described which achieved greater efficiency in rapid tran

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3

Trein, Cristina Rodrigues. "Eletroforese bidimensional em gel de poliocrilamida do plasma seminal equino e a sua relação com a congelabilidade do sêmen." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/39292.

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O objetivo deste estudo foi avaliar o perfil proteico do plasma seminal equino utilizando eletroforese bidimensional de gel de acrilamida (2D-PAGE) e determinar se algumas das proteínas presentes estavam relacionadas com a congelabilidade do sêmen. O plasma seminal foi coletado de dez garanhões, de alta e baixa congelabilidade de sêmen, provenientes do Haras Estatal da Baixa Saxônia, na cidade de Celle, na Alemanha e rotineiramente utilizados em programas de inseminação artificial. Vinte e cinco bandas proteicas foram encontradas nos géis bidimensionais (12%) e sete delas foram identificadas p

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Apraiz, Larrucea Itxaso. "Development and application of a proteomic approach to the assessment of pollution in the marine environment." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-26150.

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Today, assessment of the health of coastal waters is recognized as being important for both the conservation of nature and well-being of humans. Anthropogenic pollution has been the focus of extensive research for some time and a variety of programs for the monitoring and assessment of environmental pollution have been developed. Determination of the levels of pollution in sensitive ‘sentinels’ such as mussels, allows monitoring of these levels in a given area over a prolonged period of time. Furthermore, the biological effects of pollution are reflected in a series of biomarkers, none of whic

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5

Coaker, Gitta Laurel. "Genetic and biochemical characterization of resistance to bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1069188955.

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6

Amelina, Hanna. "Proteomics in biomarker research : Insights into the effects of aging and environment on biological systems." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-56483.

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Proteomics is the global analysis of proteins that covers a broad range of technologies aimed at determining the identity and quantity of proteins expressed in the cell, their three-dimensional structure and interaction partners. In contrast to genome, proteome reflects more accurately on the dynamic state of the cell, tissue, or an organism. Therefore much is expected from proteomics to yield better disease markers for early diagnosis and therapy monitoring, as well as biomarkers that would indicate environmental exposure or provide prediction of biological age. In this thesis, I have develop

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Qiu, Linghua. "Differentially Expressed Proteins in the Pancreas of Diabetic Mice." Ohio University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1125866599.

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8

ARBA, MORENA. "Caratterizzazione proteomica di fluidi e tessuti in diverse condizioni fisio-patologiche." Doctoral thesis, Università degli Studi di Cagliari, 2015. http://hdl.handle.net/11584/266808.

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This thesis has been focused on the proteomic characterization of human saliva from donors of different ages, starting from birth up to adult age, and pediatric brain tumor tissues. The first study has been performed in order to compare the acid-insoluble fraction of saliva from preterm with at-term newborns and adults and establish if differences exist. In the second study medulloblastoma and pilocytic astrocytoma pediatric brain tumor extracts have been compared. In both studies 2- DE analysis was coupled with high resolution tandem mass spectrometry (MS/MS). The proteomic characteriz

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9

Kierul, Kinga. "Comprehensive proteomic study of Bacillus amyloliquefaciens strain FZB42 and its response to plant root exudates." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16805.

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Bacillus amyloliquefaciens FZB42 ist ein frei lebendes Bakterium, das Pflanzenwurzeln besiedelt und das Pflanzenwachstum durch viele verschiedene Wirkmechanismen anregt. In dieser Arbeit wurden die molekularen Grundlagen dieser positiven Wirkungen, die dieses „Pflanzenwachstum fördernde Rhizobakterium“ (PGPR) auf seine Wirte ausübt, untersucht. Um den gegenseitigen Austausch von B. amyloliquefaciens und seinen Wirtspflanzen zu entschlüsseln, wurden umfangreiche Proteomstudien durchgeführt. Es wurden Referenzkarten der extrazellulären und zytosolischen Proteinfraktionen erstellt. Die größte A

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10

Rajagopal, Meena Uma. "METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS." UKnowledge, 2006. http://uknowledge.uky.edu/gradschool_diss/292.

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The development of a highly sensitive ruthenium-based fluorescent staining solution isdescribed in this dissertation. The in-house synthesized ruthenium complex (RuMS)containing both sulfonated and non-sulfonated ligand has detection limit of 1 ng ofprotein that is better than colloidal coomassie, silver and ruthenium complex containingall sulfonated ligands (RuBPS). RuMS stain has 100-fold dynamic range and does notinterfere with subsequent mass spectral identification of proteins. The capability of inhousesynthesis of the staining solution makes it a viable cost-effective alternative to thee

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11

Hadjem, Ammar Saïd. "Traitement d'image du gel d'électrophorèse bidimensionnelle." Nancy 1, 1988. http://www.theses.fr/1988NAN10143.

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L'objectif de ce travail est le traitement d'images du gel d'électrophorès bidimensionnelle (détection des taches de protéines, détermination automatique du nombre de taches de protéines sur le gel, la surface de chaque tache, leur position ainsi que leur densité optique ou en d'autres termes leur intensité lumineuse respective) en utilisant l'interface de numérisation associé à une caméra vidéo et à un micro-calculateur. Le travail a consisté, d'une part à mettre en œuvre une façon pratique cette nouvelle méthode d'investigation et d'autre part, à créer le logiciel assurant un fonctionnement

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12

Anduri, Sridevi. "Differential protein expression profiles in normal and intersex male smallmouth bass determined using one- and two-dimensional gel electrophoresis a thesis presented to the faculty of the Graduate School, Tennessee Technological University /." Click to access online, 2009. http://proquest.umi.com/pqdweb?index=0&did=2000377671&SrchMode=1&sid=2&Fmt=6&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1277817194&clientId=28564.

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13

Lundblad, Dan. "Studies on the antiproliferative action of interferon : effects on proteins synthesized in the G1 and S phase of the cell cycle in 2 anchorage-dependent cell lines." Doctoral thesis, Umeå universitet, Molekylärbiologi (Teknat- och Medfak), 1991. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-100575.

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Interferons (IFNs) are a class of structurally related proteins first discovered to be produced by virus-infected cells. By now, several other inducing agents have been described. IFNs exert multiple effects on cells exemplified by the establishment of an antiviral state, inhibition of cell proliferation and alteration of different immune reactions. In the present thesis the inhibition of cellular growth concentrated on effects in the early cell cycle have been studied. The human glioma cell line 251 MG was found to be blocked in the S phase of the cell cycle upon addition of IFN both to expon

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14

Roda, Noguera Oriol. "Caracterització dels receptors de l'activador tissular del plasminogen (tPA) en càncer de pàncrees." Doctoral thesis, Universitat Pompeu Fabra, 2006. http://hdl.handle.net/10803/7100.

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El càncer de pàncrees és altament agressiu i representa la cinquena causa de mort al mon occidental. Anteriorment, en el nostre laboratori, vam identificar que el receptor tissular del plasminogen (tPA) hi està sobre-expressat i juga un paper important el la progressió tumoral. En la present tesi hem profunditzat en l'estudi del mecanisme molecular de tPA i seus receptors en aquest càncer. <br/>En primer lloc hem caracteritzat en detall la interacció de tPA amb Annexina A2 (principal receptor de tPA en endoteli i altament expressada en pàncrees) demostrant que les dades publicades sobre la seq

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15

Lomri, Abderrahim. "Effets de la pth, de la 1,25(oh)2 d3 et du tgf-b sur la synthese et l'organisation du cytosquelette des osteoblastes en culture." Paris 6, 1988. http://www.theses.fr/1988PA066369.

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16

De-Yian, Li, and 李德彥. "Salt-induced artifacts in 2-dimensional gel electrophoresis and my strategy." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/58737389253301130967.

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碩士<br>國立臺灣大學<br>生化科學研究所<br>90<br>Abstract “Proteomics” is the large-scale screening of the proteins of a cell, organism or biological fluid, a process which requires many stringently controlled steps. The core technology of proteomics is 2-dimensional (2-D) electrophoresis. At present, there is no other technique that is capable of simultaneously resolving thousands of proteins in one separation procedure. The 2-D process begins with sample preparation. Proper sample preparation is absolutely essential for a good 2-D result. Ideally, the preparation process will result i

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17

Mohanty, Swomitra. "Development of an accessible 2-dimensional micro capillary electrophoresis device for immunological studies." 2007. http://www.library.wisc.edu/databases/connect/dissertations.html.

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18

Chi, Huang Chun, and 黃鈞琦. "Construction of two sets of the human blood proteome resolved by two-dimensional electrophoresis (2-DE)." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/35100721369484752130.

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碩士<br>輔仁大學<br>生命科學系碩士班<br>96<br>As the blood is most utilized in clinical diagnosis, human plasma proteome may be the proteome drawing highest attentions. In this study, we constructed a series of 2-D gel maps to dissect human plasma proteome. To enrich minor proteins in human plasma, two depletion strategies, the self-optimized Cibacron blue/Protein A affinity chromatography (CBA) and the commercial multiple affinity removal spin cartridge (MARS), were utilized to remove abundant proteins from plasma samples. For human plasma samples, the 2-DE procedures of large format gels (18 x20 cm) were

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19

HAO, CHIU HUA, and 邱華浩. "The expression of Dihydrodiol dehydrogenase isoforms in patients with resected lung cancer and lung carcinoma cell lines detected by using 2-dimensional electrophoresis." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/92480725609806468786.

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碩士<br>中國醫藥學院<br>醫學研究所<br>91<br>Malignant neoplasm has been the primary cause of death in Taiwan since 1982. Lung cancer is the leading and the second -leading cause of cancer death among women and men, respectively, in Taiwan. Dihydrodiol dehydrogenase（DDH） is a member of aldo-keto reductase superfamily. Human tissue contains at least four forms of DDH（DDH1~DDH4） with a similar molecular mass around 36 kDa. High expression of DDH in patients with lung cancer has been shown to be a prognostic indicator of poor disease outcome.DDH overexpression in non-small cell lung cancer (NSCLC) would sugge

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20

Petráčková, Denisa. "Využití gelově-založených proteomových technik při analýze genové exprese u prokaryotních a eukaryotních modelů." Doctoral thesis, 2011. http://www.nusl.cz/ntk/nusl-312065.

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This PhD thesis showed the applicability of a gel-based proteomic separation tool, 2-D electrophoresis in three independent projects. Supplemented with results obtained using different techniques the proteomic studies enabled a global imaging of proteoms in the studied biological systems. Comparing total proteoms of E. coli 61 protein changes were identified and connected with the development of the bacterial population in the presence of an antibiotic compound, erythromycin. This classic proteomic approach included sample extraction, optimization of its 2D separation followed by 2D gel analys

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