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Journal articles on the topic '2-dimensional electrophoresis'

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Published: 2 June 2025

Last updated: 31 July 2025

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1

Lehr, Stefan, and Reiner Westermeier. "2-dimensional gel electrophoresis reloaded." Archives of Physiology and Biochemistry 119, no. 3 (2013): 93. http://dx.doi.org/10.3109/13813455.2013.812122.

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Akhremko, Anastasiya, Ekaterina Romanovna Vasilevskaya, and Liliya Fedulova. "Adaptation of two-dimensional electrophoresis for muscle tissue analysis." Potravinarstvo Slovak Journal of Food Sciences 14 (August 28, 2020): 595–601. http://dx.doi.org/10.5219/1380.

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It is important to understand the molecular mechanisms that take place in muscle tissues and to predict meat quality characteristics. One of the most popular methods is two-dimensional electrophoresis, which allows us to visualize, share and identify different molecules, including meat proteins. However, the standard conditions of this method are not universal for all types of raw material, so the authors suggest a new variation of two-dimensional electrophoresis for muscle tissue analysis. Samples were tested by the classical version of isoelectric focusing (cathode buffer in the top and anod

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Kusari, J., and P. Roy. "Molecular and genetic comparisons of two serotypes of epizootic hemorrhagic disease of deer virus." American Journal of Veterinary Research 47, no. 8 (1986): 1713–17. https://doi.org/10.2460/ajvr.1986.47.08.1713.

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SUMMARY The virus-specific double-stranded genome RNA of 2 serotypes of epizootic hemorrhagic disease of deer virus (ehdv) was evaluated by use of coelectrophoresis in polyacrylamide and agarose gel systems. The molecular weights of virion RNA segments were 0.32 to 2.57 × 106 for ehdv-1 and 0.33 to 2.54 × 106 for ehdv-2. Seven of 10 double-stranded RNA segments of the 2 serotypes had different electrophoretic mobilities in the polyacrylamide-gel electrophoresis system. Although the individual RNA segments of each serotype contained unique RNA sequences determined on the basis of 2-dimensional

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Parent, Jean-Guy, Richard Hogue, and Alain Asselin. "Glycoproteins, enzymatic activities, and b proteins in intercellular fluid extracts from hypersensitive Nicotiana species infected with tobacco mosaic virus." Canadian Journal of Botany 63, no. 5 (1985): 928–31. http://dx.doi.org/10.1139/b85-123.

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Intercellular fluid b proteins from hypersensitive Nicotiana tabacum L. cv. Xanthi-nc and N. sylvestris Speg. and Comes infected with tobacco mosaic virus were compared by two-dimensional (2-D) polyacrylamide gel electrophoresis. Except for missing bands b2, b6a, b6b, and b7b, the overall 2-D electrophoretic pattern of N. sylvestris intercellular fluid proteins was similar to the one observed with 'Xanthi-nc' tobacco. Intercellular proteins were also studied by chromatography on con-canavalin A. Glycoproteins corresponding to b6a and b7a proteins of N. tabacum and the [Formula: see text] analo

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Tsai, Shuo-Wen, Michael Loughran, and Isao Karube. "Development of a microchip for 2-dimensional capillary electrophoresis." Journal of Micromechanics and Microengineering 14, no. 12 (2004): 1693–99. http://dx.doi.org/10.1088/0960-1317/14/12/014.

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Jeng, Robert S., Shiyuan Yu, and Morris Wayman. "Isoenzyme and protein patterns of pentose-fermenting yeasts." Canadian Journal of Microbiology 33, no. 11 (1987): 1017–23. http://dx.doi.org/10.1139/m87-179.

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Soluble proteins were extracted from the vegetative cells of four pentose-fermenting yeasts, Candida shehatae, Pichia stipitis, R-1, and R-2, the R strains being of uncertain taxonomy, while the other two are culture collection yeasts. Isoenzyme patterns, protein patterns, and two-dimensional polypeptide mapping of these four strains were compared by polyacrylamide gel electrophoresis. The two R strains showed great similarity in two-dimensional polypeptide mapping, the pattern of sodium dodecyl sulfate – polyacrylamide gel electrophoresis, isoelectrofocusing, and isoenzymes, and may be one sp

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Tokur, Bahar, and Koray Korkmaz. "Electrophoretic Methods for Identifying the Species of Seafood and Its Derivatives." Food Bulletin 2, no. 2 (2023): 61–70. http://dx.doi.org/10.61326/foodb.v2i2.121.

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The identification of the species of seafood and their products, whether they are fresh or cooked, is one of the key concerns of food regulations in many countries that have a significant intake of seafood. In point of fact, a commercial fraud happens when a species that is less value is intentionally substituted for a species that is more valuable, and a sanitary fraud takes place when a product that is potentially harmful is introduced into the market. A primary responsibility of veterinary inspection of seafood products is the detection of harmful species with the aim of removing them from

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8

Shelton, G. Diane, Everett Bandman, and George H. Cardinet. "Electrophoretic comparison of myosins from masticatory muscles and selected limb muscles in the dog." American Journal of Veterinary Research 46, no. 2 (1985): 493–98. https://doi.org/10.2460/ajvr.1985.46.02.493.

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SUMMARY Myosins from canine limb muscles (triceps brachii [medial and long heads], anconeus, and extensor carpi radialis) were compared biochemically with myosins from canine masticatory muscles (temporalis and masseter). Compared with the limb muscles, the temporalis and masseter muscles had: a unique myosin isoform pattern as determined by nondenaturing pyrophosphate gel electrophoresis; unique light chains as determined by 1-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, 2-dimensional gel electrophoresis, and peptide mapping; and a unique heavy chain as determined by

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9

Guo, X., C. Zhao, F. Wang, et al. "Investigation of Human Testis Protein Heterogeneity Using 2-Dimensional Electrophoresis." Journal of Andrology 31, no. 4 (2010): 419–29. http://dx.doi.org/10.2164/jandrol.109.007534.

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10

Hochstrasser, D., V. Augsburger, T. Pun, D. Weber, C. Pellegrini, and A. F. Muller. ""High-resolution" mini-two-dimensional gel electrophoresis automatically run and stained in less than 6 h with small, ready-to-use slab gels." Clinical Chemistry 34, no. 1 (1988): 166–70. http://dx.doi.org/10.1093/clinchem/34.1.166.

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Abstract Although two-dimensional (2-D) gel electrophoresis is one of the most powerful techniques for analyzing protein mixtures, its application in routine clinical laboratories is currently limited, because it is time-consuming, complex, and relatively expensive. Here we describe a method for automatically running and staining "high-resolution" mini 2-D electrophoresis gels in less than 6 h, by using "ready-to-use" slab gels and a PhastSystem electrophoresis apparatus. We present 2-D gel electrophoretograms of 25 nL of plasma, as well as their automatic computer analysis. For comparison, a

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Deng, Ruixue, Zhaohui Lu, Yuanjia Chen, Lu Zhou, and Xinghua Lu. "Plasma Proteomic Analysis of Pancreatic Cancer by 2-Dimensional Gel Electrophoresis." Pancreas 34, no. 3 (2007): 310–17. http://dx.doi.org/10.1097/mpa.0b013e31802f2483.

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12

MARULLO, OSVALDO, ALESSIO SOGGIU, and ENRICO CAPOBIANCO. "TWO-DIMENSIONAL ELECTROPHORESIS GEL IMAGES SCAN FOR DECOMPOSITION AND DEPLETION ANALYSIS." Advances in Adaptive Data Analysis 02, no. 03 (2010): 359–71. http://dx.doi.org/10.1142/s1793536910000525.

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Two-dimensional Electrophoresis Gel Images Scan (2dEGIS) implements a mix of computational methods for processing two-dimensional electrophoresis gel images. For advancing the analysis in case-control sample studies, a multi-component decomposition-approximation approach is presented, based on: (1) A global scan aimed to detect discriminative patterns with just a few components; (2) A more localized image scan through aggregated components; (3) The exploration of specific regions with maximal localization power. The tool 2dEGIS represents a novel unifying instrument for the computational analy

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Zhang, Sheng, Ling-Ling Zhang, Kai-Kai Zhou, Yu-Jing Liu, and Zhong Zhao. "Evaluation of three types of protein extraction methods for tetraploid black locust (Robinia pseudoacacia L.) phloem tissue proteome analysis by two-dimensional electrophoresis." Analytical Methods 7, no. 3 (2015): 1008–17. http://dx.doi.org/10.1039/c4ay02038c.

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Urban-Chmiel, Renata, Marta Dec, Andrzej Puchalski, and Andrzej Wernicki. "Characterization of heat-shock proteins in Escherichia coli strains under thermal stress in vitro." Journal of Medical Microbiology 62, no. 12 (2013): 1897–901. http://dx.doi.org/10.1099/jmm.0.064857-0.

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The aim of this study was to evaluate the effect of heat stress in in vitro conditions on the induction of heat-shock protein (Hsp)70 by Escherichia coli cells, and to determine the localization of Hsps in cell fractions. The material consisted of wild strains of E. coli isolated from the digestive tract of calves, suspended in an exponential-phase culture and subjected to 41.5 °C for 2 h. Individual fractions were analysed by SDS-PAGE and two-dimensional electrophoresis. Western blotting with mouse anti-Hsp70 and anti-Hsp60 mAbs was used to identify the proteins. Electrophoretic analysis of t

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We, Jong-Sung, Hee-Soo Park, and Ki-Rok Kwon. "Proteome Analysis of various types of Panax ginseng using 2-Dimensional Electrophoresis." Journal of Korean Institute of Herbal Acupuncture 10, no. 2 (2007): 5–18. http://dx.doi.org/10.3831/kpi.2007.10.2.005.

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Robinson, P. J., I. Lefkovits, and K. Fischer Lindahl. "MOLECULAR COMPLEXITY OF Qa-2 ANTIGENS DEMONSTRATED BY TWO-DIMENSIONAL GEL ELECTROPHORESIS." European Journal of Immunogenetics 14, no. 2-3 (1987): 81–87. http://dx.doi.org/10.1111/j.1744-313x.1987.tb00366.x.

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17

Coulonval, Katia, Laurence Bockstaele, Sabine Paternot, and Pierre P. Roger. "Phosphorylations of Cyclin-dependent Kinase 2 Revisited Using Two-dimensional Gel Electrophoresis." Journal of Biological Chemistry 278, no. 52 (2003): 52052–60. http://dx.doi.org/10.1074/jbc.m307012200.

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18

Posch, Anton, Thomas Franz, Sonja Hartwig, et al. "2D-ToGo workflow: increasing feasibility and reproducibility of 2-dimensional gel electrophoresis." Archives of Physiology and Biochemistry 119, no. 3 (2013): 108–13. http://dx.doi.org/10.3109/13813455.2013.791699.

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Takano, K. J., T. Takano, Y. Yamanouchi, and Y. Katoh. "Analysis of Pressure Response of Human Cells Using 2-Dimensional Gel Electrophoresis." High Pressure Research 22, no. 3-4 (2002): 743–46. http://dx.doi.org/10.1080/08957950212425.

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20

Mainente, Federica, Gianni Zoccatelli, Marilinda Lorenzini, et al. "Red wine proteins: Two dimensional (2-D) electrophoresis and mass spectrometry analysis." Food Chemistry 164 (December 2014): 413–17. http://dx.doi.org/10.1016/j.foodchem.2014.05.051.

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21

Leven, RM, PK Schick, and AZ Budzynski. "Fibrinogen biosynthesis in isolated guinea pig megakaryocytes." Blood 65, no. 2 (1985): 501–4. http://dx.doi.org/10.1182/blood.v65.2.501.501.

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Abstract Fibrinogen synthesis was investigated in guinea pig megakaryocytes. Purified megakaryocytes were incubated with 35S-methionine in methionine-free incubation medium for 18 hours. Newly synthesized fibrinogen in megakaryocyte lysates enriched with purified carrier guinea pig fibrinogen was immunoprecipitated with a specific anti- guinea pig fibrinogen antiserum produced in rabbits. Proteins in the immunoprecipitates were analyzed with a 3.5% to 10.0% gradient polyacrylamide slab gel electrophoresis and auto-radiography. Radioactivity was detected in a protein band of 340,000 daltons. In

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Leven, RM, PK Schick, and AZ Budzynski. "Fibrinogen biosynthesis in isolated guinea pig megakaryocytes." Blood 65, no. 2 (1985): 501–4. http://dx.doi.org/10.1182/blood.v65.2.501.bloodjournal652501.

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Fibrinogen synthesis was investigated in guinea pig megakaryocytes. Purified megakaryocytes were incubated with 35S-methionine in methionine-free incubation medium for 18 hours. Newly synthesized fibrinogen in megakaryocyte lysates enriched with purified carrier guinea pig fibrinogen was immunoprecipitated with a specific anti- guinea pig fibrinogen antiserum produced in rabbits. Proteins in the immunoprecipitates were analyzed with a 3.5% to 10.0% gradient polyacrylamide slab gel electrophoresis and auto-radiography. Radioactivity was detected in a protein band of 340,000 daltons. In order to

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Mohamed Nasir, Nurdiena, Jumriah Hiji, Jaime Jacqueline Jayapalan, and Onn Haji Hashim. "Potential use of human hair shaft keratin peptide signatures to distinguish gender and ethnicity." PeerJ 8 (January 30, 2020): e8248. http://dx.doi.org/10.7717/peerj.8248.

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Background Most human hairs collected at old crime scenes do not contain nuclear DNA and are therefore of less value for forensic investigations. In the present study, hair shaft proteins were extracted from 40 healthy subjects between the ages of 21 to 40 years and profiled using gel electrophoresis-based proteomics to determine if they can be used to distinguish gender and ethnicity. Methods Extraction of the human hair shaft proteins was performed using a newly developed alkaline solubilisation method. The extracts were profiled by 2-dimensional electrophoresis and resolved protein spots we

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Cheng, Hao-Tsai, Sen-Yung Hsieh, Chang-Mu Sung, Betty Chien-Jung Pai, Nai-Jen Liu, and Carl PC Chen. "Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis." BioMed Research International 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/5185317.

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Aims. Bile is an important body fluid which assists in the digestion of fat and excretion of endogenous and exogenous compounds. In the present study, an improved sample preparation for human bile was established.Methods and Material. The method involved acetone precipitation followed by protein extraction using commercially available 2D Clean-Up kit. The effectiveness was evaluated by 2-dimensional electrophoresis (2DE) profiling quality, including number of protein spots and spot distribution.Results. The total protein of bile fluid in benign biliary disorders was 0.797 ± 0.465 μg/μL. The sa

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Straub, Christof, Jason P. Burnham, A. Clinton White, et al. "Altered Eosinophil Proteome in a Patient with Hypereosinophilia from Acute Fascioliasis." Clinical and Vaccine Immunology 18, no. 11 (2011): 1999–2002. http://dx.doi.org/10.1128/cvi.05373-11.

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ABSTRACTWe used comparative proteomics to analyze eosinophils from a patient with hypereosinophilia due to fascioliasis. Using 2-dimensional electrophoresis and mass spectrometry, we demonstrated that the eosinophil proteome was significantly altered compared to those of healthy controls.

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Kumar, Manoj, Rajendra Singh, Anil Meena, et al. "An Improved 2-Dimensional Gel Electrophoresis Method for Resolving Human Erythrocyte Membrane Proteins." Proteomics Insights 8 (January 1, 2017): 117864181770088. http://dx.doi.org/10.1177/1178641817700880.

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The 2-dimensional gel electrophoresis (2-DE) technique is widely used for the analysis of complex protein mixtures extracted from biological samples. It is one of the most commonly used analytical techniques in proteomics to study qualitative and quantitative protein changes between different states of a cell or an organism (eg, healthy and diseased), conditionally expressed proteins, posttranslational modifications, and so on. The 2-DE technique is used for its unparalleled ability to separate thousands of proteins simultaneously. The resolution of the proteins by 2-DE largely depends on the

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Goez, Manuel Mauricio, Maria Constanza Torres-Madroñero, Sarah Röthlisberger, and Edilson Delgado-Trejos. "Preprocessing of 2-Dimensional Gel Electrophoresis Images Applied to Proteomic Analysis: A Review." Genomics, Proteomics & Bioinformatics 16, no. 1 (2018): 63–72. http://dx.doi.org/10.1016/j.gpb.2017.10.001.

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JUNGBLUT, P. "Myocardial proteins in human heart failure ? Evaluation with two-dimensional electrophoresis (2-DE)." Journal of Molecular and Cellular Cardiology 23 (July 1991): S64. http://dx.doi.org/10.1016/0022-2828(91)90706-r.

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Sekhon, Simranjeet Singh, Ji-Young Ahn, Gonhyung Kim, et al. "Proteomic profiling of pig heart and lung samples using 2-dimensional gel electrophoresis." Toxicology and Environmental Health Sciences 7, no. 3 (2015): 190–93. http://dx.doi.org/10.1007/s13530-015-0237-x.

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Azri, Wassim, Amel Ennajah, and Mai Jing. "Comparative study of six methods of protein extraction for two-dimensional gel electrophoresis of proteomic profiling in poplar stems." Canadian Journal of Plant Science 93, no. 5 (2013): 895–901. http://dx.doi.org/10.4141/cjps2013-113.

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Azri, W., Ennajah, A. and Jing, M. 2013. Comparative study of six methods of protein extraction for two-dimensional gel electrophoresis of proteomic profiling in poplar stems. Can. J. Plant Sci. 93: 895–901. Protein extraction is a crucial step in two-dimensional gel electrophoresis (2-DE) analysis of proteins, since it can have significant impact on both the quantity and the quality of protein detection. The present study is a comparison between six previously published protocols of protein extraction (A, B, C, D, E, and F) aiming to determine a suitable method to extract total proteins from

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Wang, Lining. "Proteomic Profile in Glomeruli of Type-2 Diabetic KKAy Mice using 2-Dimensional Differential Gel Electrophoresis." Medical Science Monitor 20 (2014): 2705–13. http://dx.doi.org/10.12659/msm.893078.

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Swatton, J. E., S. Prabakaran, N. A. Karp, K. S. Lilley, and S. Bahn. "Protein profiling of human postmortem brain using 2-dimensional fluorescence difference gel electrophoresis (2-D DIGE)." Molecular Psychiatry 9, no. 2 (2004): 128–43. http://dx.doi.org/10.1038/sj.mp.4001475.

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Yoshikawa, Ken-ichi, Yohtaro Katagata, Shin-ichi Ansai, and Kazuo Aso. "A comparative study of keratins in several skin tumors by 2-dimensional gel electrophoresis (2-DE)." Journal of Dermatological Science 2, no. 3 (1991): 239. http://dx.doi.org/10.1016/0923-1811(91)90186-2.

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Cichy, Aleksandra, Alicja Dratwa-Chałupnik, Weronika Medeńska, Małgorzata Ożgo, and Ryszard Pikuła. "Preparation of mare's colostrum and milk sample for 2-DE separation without acetone precipitation." Acta Scientiarum Polonorum Zootechnica 19, no. 3 (2021): 71–78. http://dx.doi.org/10.21005/asp.2020.19.3.09.

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Before electrophoretic separation is performed, the samples must be dissolved in a lysis buffer (necessary to keep proteins dissolved and unbound during proteomic analyses during for a separation of proteins on polyacrylamide gels). The first step in preparing samples for proteomic analyses is their precipitation using e.g. acetone. The aim of precipitation is to obtain proteins from the sample and to remove the compounds interfering with 2-D electrophoresis. Due to difficulties in dissolving some colostrum and mare's milk samples in buffer lysis electrophoretic separation of this biological m

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Tucker, DJ, AHF Hudson, A. Laudani, RC Marshall, and DE Rivett. "Variation in goat fibre protein." Australian Journal of Agricultural Research 40, no. 3 (1989): 675. http://dx.doi.org/10.1071/ar9890675.

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The proteins from a range of cashmere, mohair, angoratcashmere crossbred and wool fibre samples were extracted at pH 8 with 8 M urea containing dithiothreitol, and were then radiolabelled by S-carboxymethylation using iodo(2-14C) acetate. The proteins from each sample were examined by two dimensional polyacrylamide gel electrophoresis in which the separation in the first dimension was according to charge at pH 8.9 and in the second dimension according to apparent molecular weight in the presence of sodium dodecyl sulfate. After electrophoresis the proteins were detected by fluorography. Protei

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Gaylinn, B. D., T. J. Eddinger, P. A. Martino, P. L. Monical, D. F. Hunt, and R. A. Murphy. "Expression of nonmuscle myosin heavy and light chains in smooth muscle." American Journal of Physiology-Cell Physiology 257, no. 5 (1989): C997—C1004. http://dx.doi.org/10.1152/ajpcell.1989.257.5.c997.

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We have compiled evidence that nonmuscle isoforms of both myosin heavy chain (NM MHC) and myosin regulatory light chain (NM LC20) are present in fully differentiated smooth muscles (SM). In swine carotid media sodium dodecyl sulfate-gel electrophoresis separated three MHC bands. The upper two bands were identified by immunoblotting as SM-specific isoforms. The lowest MHC band amounted to 14 +/- 2% of the total MHC and was electrophoretically and antigenically similar to platelet MHC. Two-dimensional gel electrophoresis of swine carotid media extracts resolved multiple LC20 species, including p

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Chapdelaine, Pierre, Jean Y. Dubé, Gilles Frenette, and Roland R. Tremblay. "In vitro translation of mRNA for arginine esterase, the major secretory protein of dog prostate, and in vitro processing of the translation product." Canadian Journal of Biochemistry and Cell Biology 63, no. 7 (1985): 705–10. http://dx.doi.org/10.1139/o85-088.

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Poly(A)+ rich RNA was isolated from prostate of adult dogs and translated in the rabbit reticulocyte lysate cell-free protein-synthesizing system. Two-dimensional gel electrophoresis of the translation products showed that a protein with a molecular weight of 31 000 was predominantly synthesized. This protein was immunoprecipitated with antibodies directed against purified arginine esterase from dog seminal plasma. mRNA isolated from the prostate of animals castrated for 1 or 2 weeks was unable to direct the synthesis of arginine esterase. However, the synthesis of the enzyme could be stimulat

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Matsuyama, T., J. Schwenzer, J. Silver, and R. Winchester. "Structural relationships between the DR beta 1 and DR beta 2 subunits in DR4, 7, and w9 haplotypes and the DRw53 (MT3) specificity." Journal of Immunology 137, no. 3 (1986): 934–40. http://dx.doi.org/10.4049/jimmunol.137.3.934.

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Abstract The class II molecules of DR4, DR7, and DRw9 haplotypes were analyzed by immunoprecipitation, followed by two-dimensional gel electrophoresis and N-terminal amino acid sequencing. By using HLA-DR chain-specific monoclonal antibodies, two distinct DR beta-chains were identified. One beta-chain, designated DR beta 2, had a characteristic acidic mobility. In all three DR types the DR beta 2-chains were indistinguishable by two-dimensional gel electrophoresis and partial N-terminal sequencing. A second DR beta-chain designated beta 1 had a more basic mobility on two-dimensional gel electr

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John, Huw A., та Ian F. Purdom. "Heterogeneity of human haptoglobin α chains detected by two-dimensional gel electrophoresis". Genetical Research 50, № 1 (1987): 17–21. http://dx.doi.org/10.1017/s0016672300023284.

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SummaryThe protein spots representing the haptoglobin α1F, α1Sand α2chains in two-dimensional gels of human plasma samples representative of the six common haptoglobin phenotypes were identified by comparing their position with those of purified haptoglobin and distinguished from other spots in the vicinity by comparison with plasma with undetectably low levels of haptoglobin. Silver staining indicated that the α1Fchain was represented by one spot in subtypes 1F-1F, 1F-1S and 1F-2, the α1Schain by three spots in subtypes 1S-1S, 1F-1S and 1S-2 and the α2chain by six spots in 1F-2, 1S-2 and 2–2

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Addeo, Francesco, Rosalba Mauriello, and Aldo Di Luccia. "A gel electrophoretic study of caprine casein." Journal of Dairy Research 55, no. 3 (1988): 413–21. http://dx.doi.org/10.1017/s0022029900028661.

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SummaryTo compare the resolving power of starch-urea gel (SUGE) and polyacrylamide-agarose gel electrophoresis (PAAGE) in caprine casein analysis, polyacrylamide gel isoelectric focusing (PAGIF) was used as reference method. The PAAGE or SUGE patterns in the first dimension were allowed to migrate by PAGIF in an orthogonal direction giving rise to two-dimensional (2-D) separations. Using this procedure, some individual bands considered to be homogeneous by SUGE or PAAGE were found to be complex mixtures of casein components. A detailed analysis of the analytical capabilities of SUGE and PAAGE

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Yanagawa, S., H. Yokozeki, and K. Sato. "Origin of periodic acid-Schiff-reactive glycoprotein in human eccrine sweat." Journal of Applied Physiology 60, no. 5 (1986): 1615–22. http://dx.doi.org/10.1152/jappl.1986.60.5.1615.

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To evaluate the possible involvement of ductal blockade with periodic acid-Schiff (PAS)-positive materials in the mechanism of hidromeiosis in humans, skin slices were incubated with methacholine for 2 h and PAS-positive materials localized histologically in the ductal lumen. In 20% of the glands complete ductal blockade with PAS-positive materials was noted. The characteristics and origin of such PAS-positive glycoproteins in human sweat were then studied using various electrophoretic techniques. One-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis (1-D SDS-PAGE) demonstra

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Schauder, Stephan, Lucia Penna, Adeline Ritton, Catherine Manin, Fabienne Parker, and Geneviève Renauld-Mongénie. "Proteomics Analysis by Two-Dimensional Differential Gel Electrophoresis Reveals the Lack of a Broad Response of Neisseria meningitidis to In Vitro-Produced AI-2." Journal of Bacteriology 187, no. 1 (2005): 392–95. http://dx.doi.org/10.1128/jb.187.1.392-395.2005.

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ABSTRACT To investigate the effect of the autoinducer AI-2 on protein expression in Neisseria meningitidis, a luxS mutant of strain MC58 was grown in the presence and absence of in vitro-produced AI-2, and differential protein expression was assessed by two-dimensional differential gel electrophoresis. N. meningitidis did not show a global response to AI-2 signaling activity.

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Akhremko, A. G., and E. S. Vetrova. "Comparative proteomic study of pig muscle proteins during growth and development of an animal." Theory and practice of meat processing 6, no. 4 (2022): 320–27. http://dx.doi.org/10.21323/2414-438x-2021-6-4-320-327.

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The production of high-quality pork is closely related to the growth and development of muscle tissue. The present article provides a comparative proteomic research of l. dorsi, b. femoris, m. brachiocephalicus during the pigs’ growth and development (at age of 60 days and 180 days). This work was supported by data of electrophoretic methods: one-dimensional electrophoresis according to Laemmli with densitometric assessment in the ImageJ software and two-dimensional electrophoresis according to O’Farrell method with its further processing on the software ImageMaster. The mass spectrometric ide

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Mobbs, Charles, Joshua Berman, Marianne Marquardt, and Donald Pfaff. "Comprehensive polypeptide analysis of microdissected rat brain areas: combining 2-dimensional gel electrophoresis with 2-dimensional HPLC and immunoanalysis and sequencing procedures." Journal of Neuroscience Methods 29, no. 1 (1989): 5–15. http://dx.doi.org/10.1016/0165-0270(89)90103-9.

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Rodríguez-Vázquez, Raquel, Daniel Mouzo, and Carlos Zapata. "Phosphoproteome Analysis Using Two-Dimensional Electrophoresis Coupled with Chemical Dephosphorylation." Foods 11, no. 19 (2022): 3119. http://dx.doi.org/10.3390/foods11193119.

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Abstract:

Protein phosphorylation is a reversible post-translational modification (PTM) with major regulatory roles in many cellular processes. However, the analysis of phosphoproteins remains the most challenging barrier in the prevailing proteome research. Recent technological advances in two-dimensional electrophoresis (2-DE) coupled to mass spectrometry (MS) have enabled the identification, characterization, and quantification of protein phosphorylation on a global scale. Most research on phosphoproteins with 2-DE has been conducted using phosphostains. Nevertheless, low-abundant and low-phosphoryla

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Valcu, Cristina-Maria, Céline Lalanne, Gerhard Müller-Starck, Christophe Plomion, and Katja Schlink. "Protein Polymorphism between 2 Picea abies Populations Revealed by 2-Dimensional Gel Electrophoresis and Tandem Mass Spectrometry." Journal of Heredity 99, no. 4 (2008): 364–75. http://dx.doi.org/10.1093/jhered/esn007.

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LIANG, XU, JING-RONG WANG, KAM-WAI V. WONG, et al. "Optimization of 2-dimensional gel electrophoresis for proteomic studies of solid tumor tissue samples." Molecular Medicine Reports 9, no. 2 (2013): 626–32. http://dx.doi.org/10.3892/mmr.2013.1815.

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LIU, Zhiyuan, Jianrong LI, Xuepeng LI, et al. "Establishment of two-dimensional electrophoresis(2-DE)technique in muscle proteome of Fenneropenaeus chinensis." Journal of Fisheries of China 37, no. 2 (2013): 288. http://dx.doi.org/10.3724/sp.j.1231.2013.38099.

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Pattarakitkhomjon, S., S. Wongkham, W. Yutanawiboonchai, and C. Wongkham. "Analysis of plasma proteins derived from cholangiocar-cinoma patients using 2-dimensional gel electrophoresis." Biochemical Society Transactions 28, no. 5 (2000): A226. http://dx.doi.org/10.1042/bst028a226c.

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Rouquié, David, Annabelle Capt, William H. Eby, Vaithilingam Sekar, and Corinne Hérouet-Guicheney. "Investigation of endogenous soybean food allergens by using a 2-dimensional gel electrophoresis approach." Regulatory Toxicology and Pharmacology 58, no. 3 (2010): S47—S53. http://dx.doi.org/10.1016/j.yrtph.2010.09.013.

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