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Journal articles on the topic '2D-PAGE'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Matera, Robert, Katalin V. Horvath, Hari Nair, Ernst J. Schaefer, and Bela F. Asztalos. "HDL Particle Measurement: Comparison of 5 Methods." Clinical Chemistry 64, no. 3 (2018): 492–500. http://dx.doi.org/10.1373/clinchem.2017.277632.

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Abstract BACKGROUND HDL cell cholesterol efflux capacity has been documented as superior to HDL cholesterol (HDL-C) in predicting cardiovascular disease risk. HDL functions relate to its composition. Compositional assays are easier to perform and standardize than functional tests and are more practical for routine testing. Our goal was to compare measurements of HDL particles by 5 different separation methods. METHODS HDL subfractions were measured in 98 samples using vertical auto profiling (VAP), ion mobility (IM), nuclear magnetic resonance (NMR), native 2-dimensional gel electrophoresis (2

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Chorney, M. J., J. S. Tung, Y. Bushkin, and F. W. Shen. "Structural characteristics of Tla products." Journal of Experimental Medicine 162, no. 3 (1985): 781–89. http://dx.doi.org/10.1084/jem.162.3.781.

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Biochemical study of thymus leukemia antigen (TL) from thymocytes of various Tla genotypes and from leukemia cells revealed features that, given present evidence, are peculiar to TL among class I products of the H-2:Qa:Tla region of chromosome 17. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of TL from thymocytes of all TL+ mouse strains, precipitated by anti-TL antiserum or monoclonal antibodies, showed two closely migrating bands of equal intensity in the heavy (H) chain position (45-50,000 mol wt). Comparison of these two bands by two-dimensional isoelectric focusing

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Hanneken, Marina, Doreen Ackermann, Simone König, and Günter Thesseling. "Koordinatenbestimmung von Proteinpunkten in 2D-PAGE-Gelen." BIOspektrum 20, no. 6 (2014): 655–57. http://dx.doi.org/10.1007/s12268-014-0503-5.

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4

Vijayendran, Chandran, Sebastian Burgemeister, Karl Friehs, Karsten Niehaus, and Erwin Flaschel. "2DBase: 2D-PAGE database of Escherichia coli." Biochemical and Biophysical Research Communications 363, no. 3 (2007): 822–27. http://dx.doi.org/10.1016/j.bbrc.2007.09.050.

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Pietrogrande, Maria Chiara, Nicola Marchetti, Francesco Dondi, and Pier Giorgio Righetti. "Decoding 2D-PAGE complex maps: Relevance to proteomics☆." Journal of Chromatography B 833, no. 1 (2006): 51–62. http://dx.doi.org/10.1016/j.jchromb.2005.12.051.

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Griebel, Anja, Christian Obermaier, Reiner Westermeier, Martin Moche, and Knut Büttner. "Fluoreszenzmarkierung von Proteinen für 1D- und 2D-PAGE." BIOspektrum 19, no. 6 (2013): 653–55. http://dx.doi.org/10.1007/s12268-013-0375-0.

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Li, Feng, Françoise Seillier-Moiseiwitsch, and Valeriy R. Korostyshevskiy. "Region-based statistical analysis of 2D PAGE images." Computational Statistics & Data Analysis 55, no. 11 (2011): 3059–72. http://dx.doi.org/10.1016/j.csda.2011.05.013.

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Sutton, C. A., M. W. Shirley, and M. H. Wisher. "Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis." Parasitology 99, no. 2 (1989): 175–87. http://dx.doi.org/10.1017/s0031182000058613.

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SummaryTwo dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS–PAGE) has been used to produce ‘fingerprint’ maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few differences were detected between strains of the same species. Alterations to the polypeptide array associated with the stage of sporulation of the oocysts were observed. lodination of sporozoites, 2D SDS–PAGE, autoradiography and immunoblotting techniques were combined to identify polypeptides with a su

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Pavlicevic, Milica, Sladjana Stanojevic, and Biljana Vucelic-Radovic. "Influence of extraction method on protein profile of soybeans." Chemical Industry 67, no. 4 (2013): 687–94. http://dx.doi.org/10.2298/hemind120919115p.

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Comparison between protein profiles of soybean obtained by commonly used methods of extraction (Tris buffer and Tris-urea buffer) with methods used for extraction of plant proteins for 2D PAGE analysis (direct solubilization in IEF buffer, acetone extraction, phenol extraction, extraction with urea solubilization buffer and thiourea-urea extraction) was investigated. 2D profiles of samples extracted directly in IEF buffer, in urea solubilization buffer and in acetone were characterized with low number of spots. Analysis of 2D PAGE profiles of Tris buffer and Tris-urea buffer extracts showed hi

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Tao, R., H. Yamane, H. Sassa, et al. "Stylar Proteins Associated with Gametophytic Self-incompatibility in the Prunus." HortScience 32, no. 3 (1997): 514E—514. http://dx.doi.org/10.21273/hortsci.32.3.514e.

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Stylar proteins of four Prunus species, P. avium, P. dulcis, P. mume, and P. salicina, were surveyed by 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-proteins associated with gametophytic SI in the Prunus. All four S-allelic products tested for P. dulcis could be identified in the highly basic zone of the gel. These S-proteins had Mr of about 28–30 kDa and reacted with the anti-S4-serum prepared from Japanese pear (Pyrus serotina). Two of six S-allelic products tested for P. avium could be also identified in the 2D-PAGE profiles, with roughly the sa

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11

Wang, Yong, Yi Ren Jiang, and Li Qin. "Using 2D-PAGE and Mass Spectrometry (LC-MS/MS) for Embryo Proteins Analysis in Antheraea pernyi." Advanced Materials Research 884-885 (January 2014): 556–59. http://dx.doi.org/10.4028/www.scientific.net/amr.884-885.556.

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To study the dynamic variation of protein components and contents inAntheraea pernyi, 2D-PAGE, in this job, was used to investigate embryo development. Some special protein spots expression profiles changed obviously were analyzed by mass spectrometry. 2D-PAGE results showed that the protein spots increased from 370 at the development stage of 12 h to 422 at 300 h. Eighteen special protein spots were identified byLC-MS/MS, and 8 kinds of proteins are found to include heat shock proteins families, Vitellogenin, KK-42-binding protein, actin-depolymerizing factor 1, etc., which are mainly involve

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Nguyen, Thien An, and Jaejin Lee. "Simplified Two-Dimensional Generalized Partial Response Target of Holographic Data Storage Channel." Applied Sciences 12, no. 8 (2022): 4070. http://dx.doi.org/10.3390/app12084070.

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With a high capacity and fast data access rate, holographic data storage (HDS) is a potential candidate for future storage systems. However, for page-oriented data processing, two-dimensional (2D) interference appears intensely in the HDS systems. Therefore, the new 2D generalized partial response (GPR) target is introduced to estimate the 2D interference. In addition, we also propose a method to analyze the 2D GPR target into two serial one-dimensional (1D) GPR targets. It makes us design a simple detection scheme composed of two serial 1D detectors instead of a complicated 2D detector. In si

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Nishioka, Keiji, Yusuke Kato, Shin-ichiro Ozawa, Yuichiro Takahashi, and Wataru Sakamoto. "Phos-tag-based approach to study protein phosphorylation in the thylakoid membrane." Photosynthesis Research 147, no. 1 (2020): 107–24. http://dx.doi.org/10.1007/s11120-020-00803-1.

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AbstractProtein phosphorylation is a fundamental post-translational modification in all organisms. In photoautotrophic organisms, protein phosphorylation is essential for the fine-tuning of photosynthesis. The reversible phosphorylation of the photosystem II (PSII) core and the light-harvesting complex of PSII (LHCII) contribute to the regulation of photosynthetic activities. Besides the phosphorylation of these major proteins, recent phosphoproteomic analyses have revealed that several proteins are phosphorylated in the thylakoid membrane. In this study, we utilized the Phos-tag technology fo

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Issaq, Haleem J., and Timothy D. Veenstra. "Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives." BioTechniques 44, no. 5 (2008): 697–700. http://dx.doi.org/10.2144/000112823.

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15

Bäck, Peter, Sofia Bengtsson, and Peter James. "Automated PreScan Function for Scanning Fluorescently Stained 2D-PAGE Gels." Journal of Proteome Research 4, no. 5 (2005): 1511–15. http://dx.doi.org/10.1021/pr0500408.

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MIKAMI, Satoshi, Tadashi KISHIMOTO, Hidetaka HORI, and Toshiaki MITSUI. "Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins." Bioscience, Biotechnology, and Biochemistry 66, no. 5 (2002): 1170–73. http://dx.doi.org/10.1271/bbb.66.1170.

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Nowacka, Martyna, Paulina Jackowiak, Agnieszka Rybarczyk, et al. "2D-PAGE as an effective method of RNA degradome analysis." Molecular Biology Reports 39, no. 1 (2011): 139–46. http://dx.doi.org/10.1007/s11033-011-0718-1.

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18

Müller, D. R., P. Schindler, M. Coulot, H. Voshol, and J. van Oostrum. "Mass spectrometric characterization of stathmin isoforms separated by 2D PAGE." Journal of Mass Spectrometry 34, no. 4 (1999): 336–45. http://dx.doi.org/10.1002/(sici)1096-9888(199904)34:4<336::aid-jms765>3.0.co;2-u.

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19

Huber, Sylvia, Sabine Minnebusch, Stefan Wuertz, Peter A. Wilderer, and Brigitte Helmreich. "Impact of different substrates on biomass protein composition during wastewater treatment investigated by two-dimensional electrophoresis." Water Science and Technology 37, no. 4-5 (1998): 363–66. http://dx.doi.org/10.2166/wst.1998.0667.

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The influence of different influent substrates on biomass protein composition was examined by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Activated sludge from six sequencing batch reactors (SBRs) was investigated; four reactors were fed with model substrates and two received effluents from a wood milling process and a paper production process, respectively. Our investigations showed that in 2D-PAGE complex substrates caused a less diverse protein pattern than model wastewater composed of simple and low molecular weight compounds. This may be caused by complex formation by hi

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ÁLVAREZ-GARCÍA, G., A. PITARCH, A. ZABALLOS, et al. "The NcGRA7gene encodes the immunodominant 17 kDa antigen ofNeospora caninum." Parasitology 134, no. 1 (2006): 41–50. http://dx.doi.org/10.1017/s0031182006001284.

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ANeospora caninum17–19 kDa antigenic protein fraction (p17) in one-dimensional polyacrylamide gel electrophoresis (SDS-PAGE) is the immunodominant antigen recognized by sera from bovines naturally infected byN. caninum. To identify the proteins making up the p17 fraction, we screened a newN. caninumtachyzoite cDNA library with an affinity-purified antibody against p17 (APA17). We isolated several cDNA clones with 100% sequence identity to the NcGRA7gene. This previously described gene encodes a dense granule protein with an apparent molecular mass of 33 kDa. A second line of evidence emerged t

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21

Krause, Ingolf, Ursula M�ller, and Hans-Dieter Belitz. "Charakterisierung von Weizensorten durch SDS-Polyacrylamidgel-Elektrophorese (SDS-PAGE) und zweidimensionale Elektrophorese (2D-PAGE) der Glutenine." Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung 186, no. 5 (1988): 398–406. http://dx.doi.org/10.1007/bf01127299.

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22

Tiwari, Alka, W. Paul Williams, and Xueyan Shan. "MatGel: A MATLAB program for quantitative analysis of 2D polyacrylamide electrophoresis (2D-PAGE) protein gel images." MethodsX 9 (2022): 101930. http://dx.doi.org/10.1016/j.mex.2022.101930.

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23

Xing, Weirong, Yian Chen, Anakha Udayakumar, Haibo Zhao, and Subburaman Mohan. "Leucine-Rich Repeat Kinase 1 Signaling Targets Proteins Critical for Endosome/Lysosome Sorting and Trafficking in Osteoclasts." Biology 14, no. 4 (2025): 326. https://doi.org/10.3390/biology14040326.

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Global knockout (KO) of the Lrrk1 gene in mice causes severe osteopetrosis because of the failure of osteoclasts to resorb bone. The molecular mechanism of LRRK1 regulation of osteoclast function is not fully understood. Here, we performed a 2D DIGE phosphor-proteomics analysis to identify potential LRRK1 targets in osteoclasts. Splenocytes from Lrrk1 KO and wild-type (WT) mice were differentiated into osteoclasts for protein extraction. Lysates from Lrrk1 KO and WT cells were labeled with Cy3- and Cy5-dye, respectively. Labeled proteins were mixed and analyzed on the same 2D SDS PAGE for prot

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24

Cadoni, Mariano, and Andrea P. Sanna. "Unitarity and Page Curve for Evaporation of 2D AdS Black Holes." Entropy 24, no. 1 (2022): 101. http://dx.doi.org/10.3390/e24010101.

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We explore the Hawking evaporation of two-dimensional anti-de Sitter (AdS2), dilatonic black hole coupled with conformal matter, and derive the Page curve for the entanglement entropy of radiation. We first work in a semiclassical approximation with backreaction. We show that the end-point of the evaporation process is AdS2 with a vanishing dilaton, i.e., a regular, singularity-free, zero-entropy state. We explicitly compute the entanglement entropies of the black hole and the radiation as functions of the horizon radius, using the conformal field theory (CFT) dual to AdS2 gravity. We use a si

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Manabe, Takashi. "The technique of 2D-PAGE for the analysis of native proteins." SEIBUTSU BUTSURI KAGAKU 34, no. 6 (1990): 317–20. http://dx.doi.org/10.2198/sbk.34.317.

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26

Skvortsov, V. S., N. N. Alekseychuk, and A. V. Rybina. "Correction of the electrophoretic shift in virtual 2D SDS-PAGE electrophoresis." Biomeditsinskaya Khimiya 63, no. 3 (2017): 278–83. http://dx.doi.org/10.18097/pbmc20176303278.

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Virtual electrophoresis in proteomics can be used to search localization of proteins and their proteoforms (especially those existing in low concentrations), to identify proteoforms found in experiments etc. Although the problem of predicting the isoelectric point is well studied, the need of electrophoretic shift correction is usually ignored. Researchers simply use the brutto molecular weight of the protein. In this study four data sets taken from the literature sources and the SWISS-2DPAGE database have been used to build correction equations for prediction of the electrophoretic shift (123

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27

Hagen-Ansert, Sandra. "Beyond the Page: Creating a 3D Understanding of a 2D Image." Journal of Diagnostic Medical Sonography 20, no. 2 (2004): 147–49. http://dx.doi.org/10.1177/8756479304263207.

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Arrigoni, Giorgio, Celine Fernandez, Cecilia Holm, Michaela Scigelova, and Peter James. "Comparison of MS/MS Methods for Protein Identification from 2D-PAGE." Journal of Proteome Research 5, no. 9 (2006): 2294–300. http://dx.doi.org/10.1021/pr0601281.

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Moerman, P. P., K. Sergeant, G. Debyser, I. Timperman, B. Devreese, and B. Samyn. "Automation of C-terminal sequence analysis of 2D-PAGE separated proteins." EuPA Open Proteomics 3 (June 2014): 250–61. http://dx.doi.org/10.1016/j.euprot.2014.03.004.

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Reisinger, Veronika, and Lutz Andreas Eichacker. "How to Analyze Protein Complexes by 2D Blue Native SDS-PAGE." PROTEOMICS 7, S1 (2007): 6–16. http://dx.doi.org/10.1002/pmic.200700205.

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Katsani, K. R., P. Tsiboli, K. Anagnostopoulos, H. Urlaub, and T. Choli-Papadopoulou. "Identification of the 50S Ribosomal Proteins from the Eubacterium Thermus thermophilus." Biological Chemistry 381, no. 11 (2000): 1079–87. http://dx.doi.org/10.1515/bc.2000.133.

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Abstract The total protein mixture from the 50S subunit (TP-50) of the eubacterium Thermus thermophilus was characterized after blotting onto PVDF membranes from two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and sequencing. The proteins were numbered according to their primary structure similarity with their counterparts from other species. One of them has been marked with an asterisk, namely L*23, because unlike the other known ribosomal proteins it shows a very low degree of homology. A highly acidic 5S rRNA binding protein, TL5, was characterized and compared with the availab

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Centlow, Magnus, Stefan R. Hansson, and Charlotte Welinder. "Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction." Journal of Biomedicine and Biotechnology 2010 (2010): 1–9. http://dx.doi.org/10.1155/2010/458748.

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The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen. Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation protocol for placental proteins was developed. Two different buffers, urea/CHAPS and Hepes, were used for solubilization in combination with six different precipitation methods. The removal of glycogen

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Nguyen, Thien An, and Jaejin Lee. "Exploiting Extrinsic Information for Serial MAP Detection by Utilizing Estimator in Holographic Data Storage Systems." Applied Sciences 15, no. 1 (2024): 139. https://doi.org/10.3390/app15010139.

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In the big data era, data are created in huge volume. This leads to the development of storage devices. Many technologies are proposed for the next generation of storage fields. However, among them, holographic data storage (HDS) has attracted much attention and has been introduced as the promising candidate to meet the increasing demand for capacity and speed. For signal processing, HDS faces two major challenges: inter-page interference (IPI) and two-dimensional (2D) interference. To access the IPI problem, we can use balanced coding, which converts user data into an intensity level with uni

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Lister, Adrian M. "Correction for Lister, The impact of Quaternary Ice Ages on mammalian evolution." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 359, no. 1452 (2004): 1953–54. http://dx.doi.org/10.1098/rstb.2004.2000.

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Correction for ‘The impact of Quaternary Ice Ages on mammalian evolution’ by A. M. Lister (Phil. Trans. R. Soc. Lond. B 357 , 1643–1667. (doi: 10.1098/rstb.2003.1436 )). On page 230, the stippling in figure 2d,e was missing. The corrected figure and its caption appear below.

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Andringa, Kelly K., Adrienne L. King, Heather B. Eccleston, et al. "Analysis of the liver mitochondrial proteome in response to ethanol and S-adenosylmethionine treatments: novel molecular targets of disease and hepatoprotection." American Journal of Physiology-Gastrointestinal and Liver Physiology 298, no. 5 (2010): G732—G745. http://dx.doi.org/10.1152/ajpgi.00332.2009.

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S-adenosylmethionine (SAM) minimizes alcohol hepatotoxicity; however, the molecular mechanisms responsible for SAM hepatoprotection remain unknown. Herein, we use proteomics to determine whether the hepatoprotective action of SAM against early-stage alcoholic liver disease is linked to alterations in the mitochondrial proteome. For this, male rats were fed control or ethanol-containing liquid diets ± SAM and liver mitochondria were prepared for proteomic analysis. Two-dimensional isoelectric focusing (2D IEF/SDS-PAGE) and blue native gel electrophoresis (BN-PAGE) were used to determine changes

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36

Weinkauf, Marc, Grit Hutter, Yvonne Zimmermann, et al. "RNA-Expression and Proteome Analysis Identify Complementary but Not Identical Molecular Targets in Enzastaurin-Treated Mantle Cell Lymphoma." Blood 114, no. 22 (2009): 1915. http://dx.doi.org/10.1182/blood.v114.22.1915.1915.

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Abstract Abstract 1915 Poster Board I-938 Introduction: Protein kinase C beta (PKCbeta), a pivotal enzyme in B-cell signaling and survival, is over-expressed in most cases of mantle cell lymphoma (MCL) and results in activation of PI3K/AKT pathway. Enzastaurin, an oral serine/threonine kinase inhibitor, suppresses signaling through PKCbeta/PI3K/AKT pathways, induces apoptosis, reduces proliferation, and suppresses tumor-induced angiogenesis. Aim: To optimize the treatment options with this promising inhibitor the goal of this study was to elucidate the molecular pathways altered by Enzastaurin

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Tjahyadi, Surya, and Rio Fernando. "Pembuatan Design Halaman Perusahaan pada Ami Florist." Social Engagement: Jurnal Pengabdian Kepada Masyarakat 1, no. 4 (2023): 160–68. http://dx.doi.org/10.37253/se.v1i4.8558.

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Ami Florist, located in Batam City, Riau Islands, is a partner that focuses on selling flower board services, including imported and artificial flowers. Founded in 2016 under the leadership of Johan Sun, Ami Florist faced challenges in attracting customer interest for its flower board services. In his efforts to overcome this problem, Johan felt the need to have a company page that was effective in promoting his services to the wider community. In this community service project, we aim to help Ami Florist in designing and implementing an innovative company page design using 2D design methods a

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Raberg, Matthias, Frank Reinecke, Rudolf Reichelt, et al. "Ralstonia eutropha H16 Flagellation Changes According to Nutrient Supply and State of Poly(3-Hydroxybutyrate) Accumulation." Applied and Environmental Microbiology 74, no. 14 (2008): 4477–90. http://dx.doi.org/10.1128/aem.00440-08.

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ABSTRACT Two-dimensional polyacrylamide gel electrophoresis (2D PAGE), in combination with matrix-assisted laser desorption ionization-time of flight analysis, and the recently revealed genome sequence of Ralstonia eutropha H16 were employed to detect and identify proteins that are differentially expressed during different phases of poly(3-hydroxybutyric acid) (PHB) metabolism. For this, a modified protein extraction protocol applicable to PHB-harboring cells was developed to enable 2D PAGE-based proteome analysis of such cells. Subsequently, samples from (i) the exponential growth phase, (ii)

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Fuchs, Dagmar, Isabel Winkelmann, Ian T. Johnson, Edwin Mariman, Uwe Wenzel, and Hannelore Daniel. "Proteomics in nutrition research: principles, technologies and applications." British Journal of Nutrition 94, no. 3 (2005): 302–14. http://dx.doi.org/10.1079/bjn20051458.

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The global profiling of the whole protein complement of the genome expressed in a particular cell or organ, or in plasma or serum, makes it possible to identify biomarkers that respond to alterations in diet or to treatment, and that may have predictive value for the modelling of biological processes. Proteomics has not yet been applied on a large scale in nutritional studies, yet it has advantages over transcriptome profiling techniques in that it directly assesses the entities that carry out the biological functions. The present review summarizes the different approaches in proteomics resear

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Nguyen, Thien An, and Jaejin Lee. "Serial Maximum a Posteriori Detection of Two-Dimensional Generalized Partial Response Target for Holographic Data Storage Systems." Applied Sciences 13, no. 9 (2023): 5247. http://dx.doi.org/10.3390/app13095247.

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Holographic data storage (HDS) is an emerging technology that promises to revolutionize digital data storage and access. Unlike traditional storage media such as hard drives and flash memory, HDS uses light to write and read page-oriented two-dimensional (2D) data from volume media. This allows for significantly higher densities and faster data transfer rates in HDS systems. However, 2D interference is a serious issue in HDS due to hologram dispersion during the reading process. Therefore, we present a novel detection algorithm based on maximum a posteriori (MAP) detection to mitigate 2D inter

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Perrin, Clarisse, Chantal Poirson, Patrice Bracquart, Jean-Luc Gaillard, and Christiane Guimont. "Etablissement de l'empreinte protéique de base de Streptococcus thermophilus par 2D-PAGE." Sciences des Aliments 20, no. 1 (2000): 97–104. http://dx.doi.org/10.3166/sda.20.97-104.

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Saledekh, H., F. Shekari, B. Jordan, and H. Baharvand. "Proteome Analysis of Mouse Liver Microsomal Fraction Using 2D BN/SDS-PAGE." Journal of Proteomics & Bioinformatics S2, no. 01 (2008): 118. http://dx.doi.org/10.4172/jpb.s1000093.

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Wu, Steven H., Michael A. Black, Robyn A. North, Kelly R. Atkinson, and Allen G. Rodrigo. "A Statistical Model to Identify Differentially Expressed Proteins in 2D PAGE Gels." PLoS Computational Biology 5, no. 9 (2009): e1000509. http://dx.doi.org/10.1371/journal.pcbi.1000509.

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Xia, K., M. Manning, H. Hesham, Q. Lin, C. Bystroff, and W. Colon. "Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE." Proceedings of the National Academy of Sciences 104, no. 44 (2007): 17329–34. http://dx.doi.org/10.1073/pnas.0705417104.

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Stochaj, W. R., T. Berkelman, and N. Laird. "Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: SDS-PAGE of Proteins." Cold Spring Harbor Protocols 2006, no. 28 (2006): pdb.prot4559. http://dx.doi.org/10.1101/pdb.prot4559.

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COLLINS, Richard F., Toby D. FLINT, Andreas HOLZENBURG, and Robert C. FORD. "Structural changes in photosystem II after treatment with the zero-length bifunctional cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide: an electron microscopic study." Biochemical Journal 319, no. 2 (1996): 585–89. http://dx.doi.org/10.1042/bj3190585.

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Two-dimensional (2D) crystals of photosystem II (PS II) treated with various concentrations of the zero-length crosslinker 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide (EDC) were analysed by electron microscopy in conjunction with crystallographic image processing. The preparations were characterized by SDS/PAGE and oxygen-evolution measurements, and the effectiveness of cross-linking was monitored by measuring the level of protection afforded against high concentrations of NaCl and CaCl2, which normally remove extrinsic proteins from PS II. We found that low concentrations of EDC (0.25%) in

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Natale, Massimo, Bernardetta Maresca, Paolo Abrescia, and Enrico M. Bucci. "Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ." Proteomics Insights 4 (January 2011): PRI.S7971. http://dx.doi.org/10.4137/pri.s7971.

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A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow, have prompted several groups to develop freeware systems to perform certain steps of gel analysis. Unfortunately, to the best of our knowledge none of them offer a package that performs all the steps envisaged in a 2D-GE gel analysis. Here we describe an ImageJ-based procedure, able to manage all the steps of a 2D-GE gel analysis. ImageJ is a f

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Ukolova, I. V., I. G. Kondratov, M. A. Kondakova, I. V. Lyubushkina, O. I. Grabelnykh та G. B. Borovskii. "Mitochondrial сomplexome of etiolated pea shoots". Proceedings of Universities. Applied Chemistry and Biotechnology 11, № 4 (2022): 570–80. http://dx.doi.org/10.21285/2227-2925-2021-11-4-570-580.

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Studies into mitochondrial сomplexomes in various organisms provide an insight into the native organization of proteins and metabolic pathways in the organelles of the subject under study. “Complexome” is a relatively recent concept describing the proteome of protein complexes, supercomplexes, and oligomeric proteins. Complexome analysis is performed using current electrophoretic and mass spectrometric techniques, in particular, by two-dimensional electrophoresis (2D BN/SDS-PAGE) in combination with mass spectrometry (MS). Unlike 2D IEF/SDS-PAGE, this method enables analysis of not only hydrop

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S., Arunima, Alakesh Das, Prakash Jyoti Kalita, et al. "Improved methods for total and chloroplast protein extraction from Cajanus species for two-dimensional gel electrophoresis and mass spectrometry." PLOS ONE 19, no. 8 (2024): e0308909. http://dx.doi.org/10.1371/journal.pone.0308909.

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The recent advances in pigeon pea genomics, including high-quality whole genome and chloroplast genome sequence information helped develop improved varieties. However, a comprehensive Cajanus proteome, including the organelle proteome, is yet to be fully mapped. The spatial delineation of pigeon pea proteins at sub-cellular levels and inter-organelle communication could offer valuable insights into its defense mechanism against various stresses. However, the major bottleneck in the proteomic study is the lack of a suitable method of protein extraction and sample preparation compatible with two

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Gillespie, Paul F., Yanjie Wang, Kuo Yin, et al. "Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line." Vaccines 12, no. 12 (2024): 1373. https://doi.org/10.3390/vaccines12121373.

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Background/Objectives: Host cell protein (HCP) content is a major attribute for biological and vaccine products that must be extensively characterized prior to product licensure. Enzyme Linked Immunosorbent Assay (ELISA) and Mass Spectrometry (MS) are conventional methods for quantitative host cell protein analysis in biologic and vaccine products. Both techniques are usually very tedious, labor-intensive, and challenging to transfer to other laboratories. In addition, the ELISA methodology requires 2D SDS PAGE and 2D western blot antibody reagent validation to establish reagent coverage. This

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