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Journal articles on the topic 'Ajmaline'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Polz, Leo, Helmut Schübel та Joachim Stoekigt. "Characterization of 2β(R)-17-O-AcetylajmaIan: Acetylesterase — a Specific Enzyme Involved in the Biosynthesis of the Rauwolfia Alkaloid Ajmaline". Zeitschrift für Naturforschung C 42, № 4 (1987): 333–42. http://dx.doi.org/10.1515/znc-1987-0403.

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A novel enzyme was isolated, partially purified (217-fold) and characterized from cell suspension cultures of Rauwolfia serpentina Benth. The enzyme catalyzes one of the late biochemical reactions in the biosynthesis of ajmaline by hydrolysis of 17-O-acetylated alkaloids of the ajmalan group forming the appropriate deacetylated compounds. This esterase exhibits an unusually high substrate selectivity and exclusively accepts acetylated ajmaline derivatives with the naturally occurring 2β(R)-configuration. The properties of the enzyme were determined showing an optimum pH at 7.5, an isoelectric

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2

&NA;. "Ajmaline." Reactions Weekly &NA;, no. 1370 (2011): 6. http://dx.doi.org/10.2165/00128415-201113700-00015.

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3

&NA;. "Ajmaline." Reactions Weekly &NA;, no. 1335 (2011): 6. http://dx.doi.org/10.2165/00128415-201113350-00020.

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4

Pfitzner, Artur, Leo Polz, and Joachim Stöckigt. "Properties of Vinorine Synthase — the Rauwolfia Enzyme Involved in the Formation of the Ajmaline Skeleton." Zeitschrift für Naturforschung C 41, no. 1-2 (1986): 103–14. http://dx.doi.org/10.1515/znc-1986-1-217.

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Abstract Vinorine synthase, a key enzyme in the formation of the Rauwolfia alkaloid ajmaline and its derivatives, has been isolated from Rauwolfia serpentina cell suspension cultures. The new enzyme has been 160-fold purified and characterized in detail. The synthase catalyses a single step in the biosynthesis of ajmalan alkaloids by the acetyl-coenzyme A and 16-epi-vellosimine dependent formation of vinorine, which shows the basic ajmaline skeleton. Besides a characteristic substrate specificity the major properties of the enzyme are a relatively high pH optimum (pH 8.5), a temperature optim

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5

&NA;. "Ajmaline/isoprenaline." Reactions Weekly &NA;, no. 1185 (2008): 5. http://dx.doi.org/10.2165/00128415-200811850-00014.

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6

Thapanasuta, Mananchaya, Ronpichai Chokesuwattanaskul, Pattranee Leelapatana, Voravut Rungpradubvong, and Somchai Prechawat. "T-Peak to T-End Interval for Prediction of Positive Response to Ajmaline Challenge Test in Suspected Brugada Syndrome Patients." Medical Sciences 10, no. 4 (2022): 69. http://dx.doi.org/10.3390/medsci10040069.

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Background: Brugada syndrome (BrS) is diagnosed in patients with ST-segment elevation with coved-type morphology in the right precordial leads, occurring spontaneously or after provocative drugs. Due to electrocardiographic (ECG) inconsistency, provocative drugs, such as sodium-channel blockers, are useful for unmasking BrS. Ajmaline is superior to flecainide and procainamide to provoke BrS. Prolonged T-peak to T-end (TpTe) is associated with an increased risk of ventricular arrhythmia and sudden cardiac death in Brugada syndrome patients. Objective: This study aimed to investigate the predict

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7

Hollman, A. "Reserpine and ajmaline." Heart 66, no. 6 (1991): 434. http://dx.doi.org/10.1136/hrt.66.6.434.

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8

Singh, Dhruv, Bhavana Srivastava, and Archana Sahu. "Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent." Journal of the Serbian Chemical Society 68, no. 8-9 (2003): 685–90. http://dx.doi.org/10.2298/jsc0309685s.

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Arapid and simple spectrophotometric procedure is described for the determination of ajmaline and brucine. The method is based on the development of blue colored product due to reduction of tungstate and/or molybdate in Folin Ciocalteu?s reagent (FCR) by ajmaline and brucine in alkaline medium. The color is stable for more than 48 h. The chromogenic reaction has ?max at 540 nm with molar absorptivity 1.64 x 104 and 2.37 x 103 l mol-1 cm-1 in the Beer?s law range 1?8 ?g ml-1 and 10?100 ?g ml-1 for ajmaline and brucine, respectively.

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9

Monali, V. Chavan* Prof. Poonam P. Khade Dr. Megha T. Salve. "Utilization Of Radix Rauwolfia As Therapeutic Agent: A Comprehensive Review." International Journal in Pharmaceutical Sciences 1, no. 11 (2023): 293–300. https://doi.org/10.5281/zenodo.10136807.

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Rauwolfia serpentina is an important medicinal plant in the pharmaceutical world due to the presence of its immense therapeutic properties. The plant is known for curing various disorders because of the presence of alkaloids, carbohydrates, flavonoids, glycosides, phlobatannins, phenols, resins, saponins sterols, tannins and terpenes. The plant parts, root and rhizome have been used since centuries in Ayurvedic medicines for curing a large number of diseases such as high blood pressure, mental agitation, epilepsy, traumas, anxiety, excitement, schizophrenia, sedative insomnia and insanity. The

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10

Veltmann, C., C. Wolpert, F. Sacher, et al. "Response to intravenous ajmaline: a retrospective analysis of 677 ajmaline challenges." Europace 11, no. 10 (2009): 1345–52. http://dx.doi.org/10.1093/europace/eup189.

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11

Falkenhagen, Heike, and Joachim Stöckigt. "Enzymatic Biosynthesis of Vomilenine, a Key Intermediate of the Ajmaline Pathway, Catalyzed by a Novel Cytochrome P 450-Dependent Enzyme from Plant Cell Cultures of Rauwolfia serpentina." Zeitschrift für Naturforschung C 50, no. 1-2 (1995): 45–53. http://dx.doi.org/10.1515/znc-1995-1-208.

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Abstract Microsomal preparations from Rauwolfia serpentina Benth. cell suspension cultures catalyze a key step in the biosynthesis of ajmaline -the enzymatic hydroxylation of the indole alkaloid vinorine at the allylic C-21 resulting in vomilenine. Vomilenine is an important branch-point intermediate, leading not only to ajmaline but also to several side reactions of the biosynthetic pathway to ajmaline. The investigation of the taxonomical distribution of the enzyme indicated that vinorine hydroxylase is exclusively present in ajmaline-producing plant cells. The novel enzyme is strictly depe

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12

Bieda, O. A., I. I. Konvaliuk, L. P. Mozhylevska, S. S. Lukashov, V. A. Kunakh, and S. M. Yarmoluk. "Determination of content of indole alkaloids in cell biomass of Rauwolfia serpentina Benth. ex Kurz tissue culture." Current issues in pharmacy and medicine: science and practice 14, no. 1 (2021): 73–78. http://dx.doi.org/10.14739/2409-2932.2021.1.226810.

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Cardiovascular diseases are the most common human diseases, hence, the production of cardiological (in particular, anti-arrhythmic) medications from the natural sources is an ever-actual task. Rauwolfia serpentina Benth. is a tropical fruticose plant that is able to produce and concentrate indole alkaloids, especially ajmaline and its derivatives, which are the most effective medications against ventricular arrhythmia with low side effects. Aim of the study. Determination of the qualitative and quantitative content of indole alkaloids in cell biomass of Rauwolfia serpentina tissue cultur

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13

Köppel, C., J. Tenczer, and I. Arndt. "Metabolic disposition of ajmaline." European Journal of Drug Metabolism and Pharmacokinetics 14, no. 4 (1989): 309–16. http://dx.doi.org/10.1007/bf03190117.

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14

Khromov, O. S., N. V. Dobrelia, O. V. Parshikov, et al. "THE HYPOTENSIVE ACTIVITY OF DIFERENT FRACTIONS OF THE EXTRACTS FROM TISSUE CULTURE BIOMASS OF RAUWOLFIA SERPENTINA BENTH." Fiziolohichnyĭ zhurnal 69, no. 4 (2023): 45–53. http://dx.doi.org/10.15407/fz69.04.045.

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Tissue culture of Rauwolfia serpentina is an alternative source of more than 20 indole alkaloids that have antiarrhythmic, hypotensive, psychotropic, and anti-inflammatory effects. The paper was aimed at determining the hypotensive activity of five fractions (containing different combinations of alkaloids) of the extracts from the biomass of R. serpentina tissue culture (K-27M line). To achieve this aim the following methods were used: in vitro plant tissue culture, HPLC analysis of indole alkaloids, assessment of the effects of fractions on the smooth muscle of the isolated rat thoracic aorta

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15

Rahma, Annisa Nur, Revin Rindra Aghalfi Aghalfi, Diva Selviana Panggabean, et al. "Molecular docking of alkaloid compounds from the pule pandak plant (Rauvolfia serpentina L.) as inhibitors of angiotensin-converting enzyme." Pharmacy Reports 2, no. 3 (2024): 69. https://doi.org/10.51511/pr.69.

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Hypertension is a major global health issue requiring effective treatments with minimal side effects. The angiotensin-converting enzyme (ACE) is a key target in hypertension therapy, and plant-derived compounds are being explored as potential ACE inhibitors. The pule pandak plant (Rauvolfia serpentina L.) contains alkaloid compounds that may have antihypertensive properties. This study aimed to evaluate the potential of alkaloid compounds (ajmaline, rescinnamine, reserpine, and serpentine) from the pule pandak plant as antihypertensive agents using an in silico molecular docking approach. Mole

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16

Bailey, Patrick D., Mark A. Beard, Mark Cresswell, et al. "Stereoselectivity and conformational control in the synthesis of ajmaline and epi-ajmaline alkaloids." Tetrahedron Letters 54, no. 13 (2013): 1726–29. http://dx.doi.org/10.1016/j.tetlet.2013.01.074.

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17

Lounasmaa, Mauri, and Ari M. P. Koskinen. "2D NMR Study of Ajmaline." HETEROCYCLES 24, no. 2 (1986): 331. http://dx.doi.org/10.3987/r-1986-02-0331.

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18

Ikeda, Noriaki, Kazuo Umetsu, Tsuneo Suzuki, Kunio Gonmori, and Kenkiehi Takahashi. "An Infant Fatality Involving Ajmaline." Journal of Forensic Sciences 33, no. 2 (1988): 11973J. http://dx.doi.org/10.1520/jfs11973j.

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19

Mandelburger, Daniela, Alexander Teubl, and Georg Röggla. "Ajmaline challenge in Brugada syndrome." Resuscitation 74, no. 2 (2007): 393–94. http://dx.doi.org/10.1016/j.resuscitation.2007.04.008.

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20

Kaul, U., J. C. Mohan, J. Narula, C. S. Nath, and M. L. Bhatia. "Ajmaline-Induced Torsade de pointes." Cardiology 72, no. 3 (1985): 140–43. http://dx.doi.org/10.1159/000173854.

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21

Talwar, K. K., V. Dev, and M. L. Bhatia. "Ajmaline induced intra-Hisian block." International Journal of Cardiology 15, no. 1 (1987): 109–12. http://dx.doi.org/10.1016/0167-5273(87)90298-1.

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22

Tadros, Rafik, Hanno L. Tan, Sulayman el Mathari, et al. "Predicting cardiac electrical response to sodium-channel blockade and Brugada syndrome using polygenic risk scores." European Heart Journal 40, no. 37 (2019): 3097–107. http://dx.doi.org/10.1093/eurheartj/ehz435.

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Abstract Aims Sodium-channel blockers (SCBs) are associated with arrhythmia, but variability of cardiac electrical response remains unexplained. We sought to identify predictors of ajmaline-induced PR and QRS changes and Type I Brugada syndrome (BrS) electrocardiogram (ECG). Methods and results In 1368 patients that underwent ajmaline infusion for suspected BrS, we performed measurements of 26 721 ECGs, dose–response mixed modelling and genotyping. We calculated polygenic risk scores (PRS) for PR interval (PRSPR), QRS duration (PRSQRS), and Brugada syndrome (PRSBrS) derived from published geno

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23

Khan, M. Ataullah, and Humaira Raees. "Synthetic Epimers of Ajmaline and Isoajmaline." Zeitschrift für Naturforschung B 51, no. 12 (1996): 1768–70. http://dx.doi.org/10.1515/znb-1996-1214.

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Two new epimers of ajmaline (1) and isoajmaline (2) were synthesized by means of tosylation followed by hydrolysis with NaOH/CH3OH. Displacement of tosyl with hydroxyl group yielded C21 epimers of these alkaloids. The structures were identified by various spectroscopic techniques.

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24

Rizzo, Alessandro, Gianluca Borio, Juan Sieira, et al. "Ajmaline Testing and the Brugada Syndrome." American Journal of Cardiology 135 (November 2020): 91–98. http://dx.doi.org/10.1016/j.amjcard.2020.08.024.

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25

Haverkamp, W., G. Mönnig, P. Kirchhof, L. Eckardt, M. Borggrefe, and G. Breithardt. "Torsade de pointes induced by ajmaline." Zeitschrift für Kardiologie 90, no. 8 (2001): 586–90. http://dx.doi.org/10.1007/s003920170128.

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26

Beermann, Björn, Jan L. E. Ericsson, Kjell Hellström, Bo Wengle, and Bengt Werner. "TRANSIENT CHOLESTASIS DURING TREATMENT WITH AJMALINE, AND CHRONIC XANTHOMATOUS CHOLESTASIS AFTER ADMINISTRATION OF AJMALINE, METHYLTESTOSTERONE AND ETHINYLESTRADIOL." Acta Medica Scandinavica 190, no. 1-6 (2009): 241–50. http://dx.doi.org/10.1111/j.0954-6820.1971.tb07425.x.

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27

Ahmad, S. S., Tanveer Akhtar, S. Malik, S. I. Haider, and S. H. Khan. "Conversion of ajmaline, isoajmaline, sandwicine and isosandwicine to their acylation products." Collection of Czechoslovak Chemical Communications 51, no. 10 (1986): 2240–42. http://dx.doi.org/10.1135/cccc19862240.

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Ajmaline, isoajmaline, sandwicine and isosandwicine were converted to their new acylation products in 68-94% yields using isobutyric anhydride and isovaleric anhydride. The structures of these compounds were established on the basis of their spectroscopic studies.

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28

Tadros, Rafik, Hanno L. Tan, the ESCAPE-NET Investigators for, et al. "Predicting cardiac electrical response to sodium-channel blockade and Brugada syndrome using polygenic risk scores." European Heart Journal 40, no. 37 (2019): 3097–107. https://doi.org/10.1093/eurheartj/ehz43.

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Sodium-channel blockers (SCBs) are associated with arrhythmia, but variability of cardiac electrical response remains unexplained. We sought to identify predictors of ajmaline-induced PR and QRS changes and Type I Brugada syndrome (BrS) electrocardiogram (ECG).

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29

Si, Ying-Ying, Wei-Wei Wang, Qing-Mei Feng, et al. "Neuroinflammatory inhibitors from Gardneria nutans Siebold & Zuccarini." RSC Advances 11, no. 44 (2021): 27085–91. http://dx.doi.org/10.1039/d1ra05204g.

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Four new compounds were isolated from G. nutans. Compounds 1–2 are two rare monoterpene indole alkaloids with the glucosyl moiety located at C-12 and represent the first two examples of enantiomer of ajmaline type monoterpene indole alkaloids.

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30

Padrini, Roberto, Donatella Piovan, Antonio Javarnaro, Francesco Cucchini, and Mariano Ferrari. "Pharmacokinetics and Electrophysiological Effects of Intravenous Ajmaline." Clinical Pharmacokinetics 25, no. 5 (1993): 408–14. http://dx.doi.org/10.2165/00003088-199325050-00006.

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31

Velagić, Vedran, Davor Puljević, Mislav Puljević, Borka Pezo-Nikolić, and Davor Miličić. "Brugada syndrome – first results of ajmaline testing." Cardiologia Croatica 11, no. 10-11 (2016): 437. http://dx.doi.org/10.15836/ccar2016.437.

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32

Martini, B. "The ajmaline challenge and a strange ECG." Europace 11, no. 10 (2009): 1406. http://dx.doi.org/10.1093/europace/eup224.

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33

Mellor, Greg, Ian Fellows, and Ian Williams. "‘Intrahepatic cholestatic hepatitis following diagnostic ajmaline challenge’." EP Europace 15, no. 3 (2012): 314. http://dx.doi.org/10.1093/europace/eus206.

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34

Larrey, Dominique, Dominique Pessayre, Gilles Duhamel, et al. "Prolonged cholestasis after ajmaline-induced acute hepatitis." Journal of Hepatology 2, no. 1 (1986): 81–87. http://dx.doi.org/10.1016/s0168-8278(86)80011-3.

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35

Bajaj, Rajiv, Upendra Kaul, and Jagat Narula. "Unmasking of sinus node dysfunction by ajmaline." International Journal of Cardiology 23, no. 3 (1989): 402–4. http://dx.doi.org/10.1016/0167-5273(89)90203-9.

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36

Arens, H., B. Deus-Neumann, and M. Zenk. "Radioimmunoassay for the Quantitative Determination of Ajmaline." Planta Medica 53, no. 02 (1987): 179–83. http://dx.doi.org/10.1055/s-2006-962667.

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37

Endress, Susanne, and Joachim Stöckigt. "‘One-Pot’ Synthesis of Raumacline from Ajmaline." Helvetica Chimica Acta 76, no. 7 (1993): 2544–46. http://dx.doi.org/10.1002/hlca.19930760713.

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38

Uziębło-Życzkowska, Beata, Grzegorz Gielerak, Paweł Siedlecki, and Beata Pająk. "Genetic Diversity ofSCN5AGene and Its Possible Association with the Concealed Form of Brugada Syndrome Development in Polish Group of Patients." BioMed Research International 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/462609.

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Brugada Syndrome (BS) is an inherited channelopathy associated with a high incidence of sudden cardiac death. The paper presents the discovery of new genetic variants ofSCN5Agene which might be associated with the development of a concealed form of Brugada Syndrome. The study involved a group of 59 patients (37 men) with suspected concealed form of Brugada Syndrome. Pharmacological provocation with intravenous ajmaline administration was performed. Six patients with positive test results were subjected to molecular analysis ofSCN5Agene with MSSCP method. Additionally, MSSCP genotyping was perf

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39

Srivastava, A., A. K. Tripathi, R. Pandey, R. K. Verma, and M. M. Gupta. "Quantitative Determination of Reserpine, Ajmaline, and Ajmalicine in Rauvolfia serpentina by Reversed- Phase High-Performance Liquid Chromatography." Journal of Chromatographic Science 44, no. 9 (2006): 557–60. http://dx.doi.org/10.1093/chromsci/44.9.557.

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40

Bestetti, Reinaldo B., Lucimara Z. Pinto, Edson G. Soares, Gerson Muccillo, and J. Samuel M. Oliveira. "Changes in electrocardiographic patterns at different stages of Chagas' heart disease in rats." Clinical Science 80, no. 1 (1991): 33–37. http://dx.doi.org/10.1042/cs0800033.

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1. The resting electrocardiogram was obtained from 25 Trypanosoma cruzi-infected rats 30 days after infection (phase I). The resting electrocardiogram was abnormal in 12 (group I) and normal in 13 (group II) animals. Nineteen similar but non-infected animals served as controls. Both the resting electrocardiogram and the ajmaline test were performed 120 and 350 days after infection (phases II and III, respectively). 2. With regard to the resting electrocardiogram of group I animals, left axis deviation was found in 10 of 12 (83%) in phase I, one of 12 (8%) in phase II (P <0.05) and in none i

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41

Rukachaisirikul, Thitima, Suwadee Chokchaisiri, Parichat Suebsakwong, Apichart Suksamrarn, and Chainarong Tocharus. "A New Ajmaline-type Alkaloid from the Roots of Rauvolfia serpentina." Natural Product Communications 12, no. 4 (2017): 1934578X1701200. http://dx.doi.org/10.1177/1934578x1701200408.

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A new ajmaline-type alkaloid, 21- O-methylisoajmaline (1), together with twenty-one known compounds, a mixture ofβ-sitosterol (2) and stigmasterol (3), reserpinine (4), tetrahydroalstonine (5), reserpine (6), venoterpine (7), yohimbine (8), 6'- O-(3,4,5-trimethoxybenzoyl)glomeratose A (9), isoajmaline (10), 3- epi-α-yohimbine (11), methyl 3,4,5-trimethoxy- trans-cinnamate (12), a mixture of β-sitosterol 3- O-β-D-glucopyranoside (13) and stigmasterol 3- O-β-D-glucopyranoside (14), rescidine (15), 7-deoxyloganic acid (16), ajmaline (17), suaveoline (18), (+)-tetraphyllicine (19), loganic acid (2

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42

Syed Iqbaluddin, Juwairiya, Fathima Murthuza, and Sumaiya Iqbal. "Diagnosis of Brugada Syndrome, a Rare Inherited Arrhythmogenic Disorder." Dubai Medical Journal 3, no. 2 (2020): 70–73. http://dx.doi.org/10.1159/000507572.

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Brugada syndrome (BrS) is a rare, autosomal dominant genetic disorder with mutation in the SCN5A gene. It is associated with an increased risk of arrhythmias and sudden cardiac death. BrS can be diagnosed by characteristic electrocardiogram (ECG) findings and significant events, such as syncope, palpitations, nocturnal respiratory agonia, and family history of sudden cardiac death below the age of 45 years. Special investigations, such as electrophysiology study, ajmaline provocation test, and genetic testing, play an important role in its diagnosis. This case report describes a patient who pr

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43

Veltmann, C., C. Wolpert, R. Schimpf, et al. "The ajmaline challenge and a strange ECG: reply." Europace 11, no. 10 (2009): 1406–7. http://dx.doi.org/10.1093/europace/eup254.

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44

Belhassen, Bernard, Shimon Bar-David, and Shlomo Laniado. "Diagnosis of Dual Accessory Pathways by Ajmaline Test." Cardiology 76, no. 1 (1989): 53–57. http://dx.doi.org/10.1159/000174471.

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45

Lounasmaa, Mauri, and Pirjo Hanhinen. "Biogenetic Formation of Sarpagine/Ajmaline Type Indole Alkaloids." Planta Medica 64, no. 06 (1998): 572–74. http://dx.doi.org/10.1055/s-2006-957518.

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46

Probst, Vincent, and Herve Le Marec. "Ajmaline challenge: To stop or not to stop…" Heart Rhythm 6, no. 5 (2009): 632–33. http://dx.doi.org/10.1016/j.hrthm.2009.03.009.

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47

Roten, Laurent, Nicolas Derval, Frédéric Sacher, et al. "Ajmaline attenuates electrocardiogram characteristics of inferolateral early repolarization." Heart Rhythm 9, no. 2 (2012): 232–39. http://dx.doi.org/10.1016/j.hrthm.2011.09.013.

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48

Lounasmaa, Mauri, and Pirjo Hanhinen. "ChemInform Abstract: The Ajmaline Group of Indole Alkaloids." ChemInform 32, no. 15 (2001): no. http://dx.doi.org/10.1002/chin.200115281.

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49

"Ajmaline." Reactions Weekly 1872, no. 1 (2021): 24. http://dx.doi.org/10.1007/s40278-021-01950-9.

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50

"Ajmaline." Reactions 243, no. 1 (1989): 3–4. http://dx.doi.org/10.1007/bf03283118.

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