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Dissertations / Theses on the topic 'Amino acids sensing'

Author: Grafiati
Published: 26 October 2024
Last updated: 31 July 2025

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1

Nakato, Junya. "Physiological studies on gastrointestinal sensing of peptides and amino acids." Kyoto University, 2018. http://hdl.handle.net/2433/232349.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第21148号<br>農博第2274号<br>新制||農||1058(附属図書館)<br>学位論文||H30||N5122(農学部図書室)<br>京都大学大学院農学研究科食品生物科学専攻<br>(主査)教授 金本 龍平, 教授 保川 清, 教授 谷 史人<br>学位規則第4条第1項該当
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2

Chiang, Mengying. "A Study on the Regulation of Amino Acids and Glucose Sensing Pathways in Saccharomyces cerevisiae." ScholarWorks@UNO, 2013. http://scholarworks.uno.edu/td/1713.

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Nutrient availability regulates eukaryotic cell growth. This study focuses on two signaling pathways, involved in sensing amino acids and carbon sources, which allow cells to respond appropriately to their presence. The first part of this study shows that Ssy1, a plasma membrane localized sensor in the Ssy1-Ptr3-Ssy5 (SPS) amino acid sensing pathway, can detect 19 common L-amino acids with different potencies and affinities based on the physiochemical structure of amino acids. Substituents around alpha carbon are critical for amino acid sensing by Ssy1. Furthermore, a high concentration of cys
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3

Price, Michelle B. "Functional Analysis of Plant Glutamate Receptors." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/51946.

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The plant glutamate receptors (GLRs) are homologs of mammalian ionotropic glutamate receptors (iGluRs) and are hypothesized to be potential amino acid sensors in plants. Since their first discovery in 1998, the members of plant GLRs have been implicated in diverse processes such as C/N ratio sensing, root formation, pollen germination and plant-pathogen interaction. However, the exact properties of these channels, such as the spectrum of ligands, ion specificities, and subunit compositions are still not well understood. It is well established that animal iGluRs form homo- or hetero-tetramers
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4

Hoe, Nancy Palme. "Analysis of Temperature Sensing in Yersinia pestis: A Dissertation." eScholarship@UMMS, 1994. https://escholarship.umassmed.edu/gsbs_diss/98.

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The lcrF gene of Yersinia pestis, the etiological agent of plague, encodes a transcription activator responsible for inducing expression of several virulence-related proteins (Yops) in response to temperature. The mechanism of this thermoregulation was investigated. Using a yopE::lacZ reporter fusion, lcrF-mediated thermal regulation was observed in Y. pestis and Escherichia coli. The lcrF gene was sequenced, the 30.8 kDa. LcrF protein identified and purified, and LcrF-dependent yopE-specific DNA binding activity was detected. A sequence similarity search revealed that LcrF exhibits 98% homolo
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5

Springauf, Andreas [Verfasser]. "Electrophysiological characterization of the acid sensing ion channel shark ASIC1b and identification of amino acids controlling the gating of ASIC1 / Andreas Springauf." Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2011. http://d-nb.info/1018222596/34.

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6

Pushina, Mariia. "Sensing of Anions, Amines, Diols, and Saccharides by Supramolecular Fluorescent Sensors." Bowling Green State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1558539245401457.

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7

Cardoch, Sebastian. "Computational study of single protein sensing using nanopores." Thesis, Uppsala universitet, Materialteori, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-423441.

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Identifying the protein content in a cell in a fast and reliable manner has become a relevant goal in the field of proteomics. This thesis computationally explores the potential for silicon nitride nanopores to sense and distinguish single miniproteins, which are small domains that promise to facilitate the systematic study of larger proteins. Sensing and identification of these biomolecules using nanopores happens by studying modulations in ionic current during translocation. The approach taken in this work was to study two miniproteins of similar geometry, using a cylindrical-shaped pore. I
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8

Han, Ling. "Physiology of Escherichia coli in batch and fed-batch cultures with special emphasis on amino acid and glucose metabolism." Doctoral thesis, KTH, Biotechnology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3334.

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<p>The objective of this work is to better understand themetabolism and physiology of<i>Escherichiacoli</i>(W3110) in defined medium cultures with thelong-term goal of improving cell yield and recombinant proteinproductivity.</p><p>The order of amino acid utilization in<i>E. coli</i>batch cultures was investigated in a medium with16 amino acids and glucose. Ser, Pro, Asp, Gly, Thr, Glu andAla were rapidly consumed and depleted at the end of theexponential phase, while His, Arg, Val, Met, Ile, Leu, Phe, Lysand Tyr were consumed slowly during the following linear growthphase. The uptake order co
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9

Delescluse, Julie. "MND, un transporteur d’acides aminés, acteur clef de la réponse neuronale aux acides aminés des corps pédonculés, chez l’adulte Drosophila melanogaster." Electronic Thesis or Diss., Bourgogne Franche-Comté, 2024. http://www.theses.fr/2024UBFCK029.

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Chaque organisme vivant évolue dans son écosystème, où il doit être capable de détecter et d’intégrer de multiples facteurs environnementaux (température, hygrométrie, composés chimiques organiques ou non…). Ces signaux jouent un rôle important dans la communication entre organismes. Chaque individu doit donc mettre en lien ces stimuli externes avec ses propres signaux internes (nutritionnel, métabolique, hormonal, infection…), et adapter son comportement en conséquence pour assurer sa survie et sa reproduction. Pour ce faire des mécanismes complexes de détection se sont développés, notamment
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10

Lucchesi, Pamela A. "Plasma Membrane Processes in Smooth Muscle: Characterization of Ca2+ Transport and Muscarinic Cholinergic Receptors: A Thesis." eScholarship@UMMS, 1989. https://escholarship.umassmed.edu/gsbs_diss/135.

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The thesis research was designed to study the characteristics of two important physiological processes in smooth muscle: Ca2+ transport mediated by the plasmalemmal Ca2+-ATPase and muscarinic receptor-G protein interactions. In resting smooth muscle, several Ca2+ extrusion or sequestration processes offset the passive inward leak of Ca2+. Although biochemical evidence suggests that the plasmalemmal Ca2+ pump plays a key role in this process, the precise role of this enzyme could not be proven until a reliable estimate of the inward Ca2+ leak was measured. Recent studies using dispersed smooth
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11

Saxton, Robert A. (Robert Andrew). "Mechanisms of amino acid sensing by the mTORC1 pathway." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/117880.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, June 2018.<br>Cataloged from PDF version of thesis. "June 2018."<br>Includes bibliographical references.<br>In order to coordinate growth with organismal physiology, cells must couple anabolic and catabolic metabolism with a variety of environmental cues. In humans, cell growth is controlled primarily by the mechanistic Target of Rapamycin Complex 1 (mTORC1), which senses a diverse array of environmental inputs and in turn regulates nearly all aspects of growth and metabolism. Consistent with its central role in thes
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12

Lazutkaite, Greta. "Amino acid sensing in hypothalamic tanycytes via umami taste receptors." Thesis, University of Warwick, 2018. http://wrap.warwick.ac.uk/109627/.

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Energy homeostasis is controlled in the hypothalamus. Hypothalamic tanycytes are a type of glial cell that lie in the wall of the third ventricle, between ventricular CSF and the key nuclei controlling feeding, and are potentially linked to this regulation. Tanycytes have been found to express sweet taste receptors; similar receptors exist for amino acids and could be present in tanycytes. Therefore, the aims of this work were to understand the tanycyte amino acid signalling process, determine the impact of diet on tanycyte function, and link tanycytes to energy homeostasis. Using ratiometric
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13

Chantranupong, Lynne. "Discovering regulators of the amino acid sensing pathway of mTORC1." Thesis, Massachusetts Institute of Technology, 2017. http://hdl.handle.net/1721.1/108886.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, February 2017.<br>Cataloged from PDF version of thesis. "February 2017."<br>Includes bibliographical references.<br>The mechanistic target of rapamycin complex I (mTORC1) protein kinase functions as a master regulator of growth, and its deregulation is common in human disease, including cancer and diabetes. mTORC1 integrates multiple environmental cues to control anabolic and catabolic processes. A key input is amino acids, which function to promote the translocation of mTORC1 to the lysosomal surface, its site of ac
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14

Wang, Shuyu Ph D. Massachusetts Institute of Technology. "Studies of amino acid sensing by the mTORC 1 pathway." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/106737.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2016.<br>Cataloged from PDF version of thesis. "September 2016."<br>Includes bibliographical references.<br>The mTOR complex 1 (mTORC 1) protein kinase is a master growth regulator that responds to multiple cues from the local and systemic environment surrounding the cell. Nutrients and growth factors are both required to activate mTORC 1 and to promote growth. While the mechanisms of growth factor signaling have been reasonably well established, we have only begun to unravel in recent years how amino acids signal to
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15

Trott, Amy Elizabeth. "Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/34070.

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<I>Vibrio fischeri</I>, a symbiotic bioluminescent bacterium, serves as one of the best understood model systems for a mechanism of cell-density dependent bacterial gene regulation known as quorum sensing. During quorum sensing in <I>V. fischeri</I>, an acylated homoserine chemical signal (autoinducer) is synthesized by the bacteria and used to sense their own species in a given environment. As the autoinducer levels rise, complexes form between the autoinducer and the N-terminal domain of a regulatory protein, LuxR. In response to autoinducer binding, LuxR is believed to undergo a conformatio
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16

Wang, Jiefu [Verfasser], Siegfried [Akademischer Betreuer] Ussar, Siegfried [Gutachter] Ussar, and Alfred [Gutachter] Kleusberg. "Defining the roles of Kindlin-2 and PAT2 in adipose tissue plasticity and amino acid sensing, respectively / Jiefu Wang ; Gutachter: Siegfried Ussar, Alfred Kleusberg ; Betreuer: Siegfried Ussar." München : Universitätsbibliothek der TU München, 2020. http://d-nb.info/1213447550/34.

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17

Palmer, Gregory Charles. "Investigations into the role of aromatic amino acids in quorum sensing-mediated virulence in Pseudomonas aeruginosa." Thesis, 2012. http://hdl.handle.net/2152/ETD-UT-2012-08-6200.

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Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that is a primary constituent of chronic, polymicrobial infections in the lungs of individuals with cystic fibrosis (CF). A significant consequence of CF is production of thick mucus along epithelial surfaces. In the lungs, this mucus collects and serves as an excellent growth substrate for a range of bacteria including. CF lung fluids (sputum) also enhance the virulence of P. aeruginosa, as production of a signaling molecule critical for virulence, the Pseudomonas quinolone signal (PQS), is enhanced in the presence of phenylalan
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18

Chiou, Ya-Ping, and 邱雅萍. "Development and Characterization of novel cross-linked infrared sensing phase for selective determination of negative charged amino acids." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/66980498174764581006.

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碩士<br>國立中興大學<br>化學系<br>93<br>Infrared spectroscopy provides a simple but powerful method to detect organic compounds. However, the detection of high polar compounds, such as negatively charged amino acid, is limited due to the high polarity and the negatively charged characteristics. In order to improve the selectivity and the sensitivity to the negatively charged amino acids, in this investigation, high polarity amine compounds was successfully immobilized on the internal reflection element by cross-linking method. The prepared sensing phase shows no obvious variation between pH=3 to 9 in aqu
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19

CHEN, YI-SHAO, and 陳奕劭. "Application of Aniline Oligomer Based Chemical Sensors for Anion, Cation and Amino Acid Sensing." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/e62bpp.

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碩士<br>逢甲大學<br>化學工程學系<br>106<br>We’ve successfully synthesized a series of efficient chemical sensors, including two aniline trimer based colorimetric chemical sensors. Colorimetric dual responsive sensor (CDRS) with thiourea binding site can sense CN- anion and changes in pH. Cation-Amino acid sensor (CAS) with chiral thiourea binding site can sense Ag+, Cu2+, Fe3+ and L- Tryptophan. Upon addition of CN- to CDRS in 30% aqueous DMSO, the colorimetric response of solution of CDRS showed a color change from orange to purple. For CAS, upon addition of Ag+, Cu2+, and Fe3+ to it in 50% aqueous DMSO
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20

Lo, Kai-Yin, and 羅凱尹. "Analysis of N-acyl-D-amino acid amidohydrolase operon and the metabolism of quorum-sensing factor in Variovorax paradoxus Iso1." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/40082612879373953521.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>91<br>We sequenced a gene fragment in pBluescriptII KS (+), our lab cloned before, containing N-acyl-D-amino acid amidohydrolase (N-D-AAase) from Variovorax paradoxus Iso1. Sequence analysis revealed ten open reading frames, except N-acyl-D-amino acid amidohydrolase, including intergrase, D-amino acid dehydrogenase, two regulators and three ABC transporters. The putative D-amino acid dehydrogenase (DAAO), encodes 416 amino acids, was through gene cloning, expression, and measure activity to confirm its function. We choused two expression systems-pQE30 and pET21a-to e
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21

Joseph, Alex. "Synthesis and Characterization of Functionalized Electroactive Polymers for Metal Ion Sensing." Thesis, 2014. http://etd.iisc.ac.in/handle/2005/3056.

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Metal ion contamination in surface and ground water is a major threat as it has a direct implication on the health of terrestrial and aquatic flora and fauna. Lead (Pb2+), mercury (Hg2+), cadmium (Cd2+), nickel (Ni2+), copper (Cu2+) and cobalt (Co2+) are few of these metal ions which are classified under the high risk category. Of these, lead and mercury are of greater concern, as even nanomolar concentrations can be lethal, as they can be bio-accumulated and result in physiological as well as neurological disorders. In Asian countries like India and China, heavy metal pollution is more preval
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22

Joseph, Alex. "Synthesis and Characterization of Functionalized Electroactive Polymers for Metal Ion Sensing." Thesis, 2014. http://hdl.handle.net/2005/3056.

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Metal ion contamination in surface and ground water is a major threat as it has a direct implication on the health of terrestrial and aquatic flora and fauna. Lead (Pb2+), mercury (Hg2+), cadmium (Cd2+), nickel (Ni2+), copper (Cu2+) and cobalt (Co2+) are few of these metal ions which are classified under the high risk category. Of these, lead and mercury are of greater concern, as even nanomolar concentrations can be lethal, as they can be bio-accumulated and result in physiological as well as neurological disorders. In Asian countries like India and China, heavy metal pollution is more preval
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