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Dissertations / Theses on the topic 'Artificial chromosomes'

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Published: 4 June 2021

Last updated: 25 July 2025

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1

Morroll, Shaun Michael. "Mapping of yeast artificial chromosomes from Arabidopsis chromosome 5." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308922.

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2

Heller, Raoul. "Engineering of human artificial mini-chromosomes." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360317.

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3

Mandegar, Mohammad Ali. "Analysis of artificial chromosomes in human embryonic stem cells." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:81d118c3-dd01-40e4-9fea-2c335d9f3101.

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The development of safe and efficient gene delivery systems in pluripotent human embryonic stem cells (hESc) is essential to realising their full potential for basic and clinical research. The purpose of this study was to develop an efficient, non-integrating gene expression system in pluripotent hESc using human artificial chromosomes (HAC). Similar to endogenous chromosomes, HAC are capable of gene expression, replication and segregation during cell division. Unlike retroviral-mediated gene delivery vectors, HAC do not integrate into the host genome and can encompass large genomic regions fo

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4

Mann, Kathryn Louise. "Investigation of possible cloning hosts for mammalian artificial chromosomes." Thesis, Imperial College London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393888.

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5

Meaney, Paul James. "Mapping the Plasmodium falciparum genome with yeast artificial chromosomes." Thesis, University of Edinburgh, 1995. http://hdl.handle.net/1842/12640.

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Yeast Artificial Chromosome cloning vectors, with their capacity to maintain up to 1 Mb of cloned DNA in a stable form, have proved extremely useful in mapping the genomes of higher eukaryotes. These vectors possess features which can circumvent some of the problems associated with classical molecular manipulation in <I>Plasmodium falciparum. </I>The research presented in this thesis is aimed at contributing to genome mapping in <I>P. falciparum. </I>The primary objective is the construction of a complete, detailed YAC- based physical map of chromosome 6, with a resolution of 10 Kb. To accompl

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6

Chan, David Yiu Leung. "Analysis of artificial chromosomes and factors affecting stability in murine and human cultured and embryonic stem cells." Thesis, University of Oxford, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.568074.

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Human Artificial Chromosomes (HAC) are fascinating extrachromosomal molecules that stay independently from the host genome and are capable of segregating as efficiently as endogenous chromosomes. It has been proven that HAC are potential tools for both basic chromosome behavioural research and agents for gene therapy purposes. My DPhil project is divided into two main themes. The first theme was to develop a novel artificial chromosome in mouse embryonic stem cells. The second theme was to understand the factors affecting chromosome stability which may also affect the efficiency of artificial

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7

Alazami, Anas Muhammad. "The establishment and validation of artificial chromosomes in mammalian cells." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.400456.

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8

Tonga, Lavon Paongo Hutt-Fletcher Lindsey M. "Development of an Akata-based bacterial artificial chromosome." Diss., UMK access, 2006.

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Thesis (Ph. D.)--School of Biological Sciences. University of Missouri--Kansas City, 2006.<br>"A dissertation in molecular biology and biochemistry and cell biology and biophysics." Advisor: Lindsey M. Hutt-Fletcher. Typescript. Vita. Title from "catalog record" of the print edition Description based on contents viewed Nov. 9, 2007. Includes bibliographical references (leaves 89-103). Online version of the print edition.

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9

Howe, Steven James. "orip-EBNA-1 artificial chromosomes as expression vectors for gene therapy." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271147.

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10

Francis, Michael J. "Physical mapping around the SMA gene using yeast artificial chromosomes (YACs)." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259879.

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11

Davies, Nicholas Paul. "Modification, transfer and expression of yeast artificial chromosomes carrying human immunoglobulin genes." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318326.

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12

Rudd, Mary Katharine. "Organization, evolution and function of alpha satellite DNA at human centromeres." Case Western Reserve University School of Graduate Studies / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=case1091493781.

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13

Meyer, Knut. "Positional cloning of the ABI1 locus of Arabidopsis thaliana using yeast artificial chromosomes /." [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10974.

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14

White, Jacqueline Katie. "Analysis of human NRAMP, IL8R and V1L1 genes (2q35) using yeast artificial chromosomes." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339595.

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15

Van, Brabant Anja Josifa. "Characterizing YAC replication : identification and deletion of replication origins within a human DNA insert /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/10274.

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16

McGuigan, Amanda Jane. "Functional analysis of DNA cloned in yeast artificial chromosomes for replication and centromere function in mammalian cells." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299885.

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17

Kong, Sim Yee. "Plasmid DNA and bacterial artificial chromosomes processing for gene therapy and vaccination : studies on membrane sterile filtration." Thesis, University College London (University of London), 2007. http://discovery.ucl.ac.uk/1444899/.

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Plasmids currently applied in clinical trials are generally < 20 kb, but the interest in larger circular vectors is rising. However, due to their size and character, filterability and irreversible damage intensified by elongational shear are major concerns during a sterilising filtration procedure. Therefore, key parameters affecting the normal-flow membrane filtration performance of solutions containing purified plasmid DNA and bacterial artificial chromosomes were investigated in this study. Two small scale filtration systems were designed to enable information on material properties to be o

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18

Somridhivej, Benjaporn Liu Zhanjiang. "Characterization, polymorphism assessment, and database construction for microsatellites from BAC end sequences of catfish a resource for integration of linkage and physical maps /." Auburn, Ala., 2007. http://repo.lib.auburn.edu/Send%2011-10-07/SOMRIDHIVEJ_BENJAPORN_30.pdf.

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19

McDowell, Erin. "Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 106 p, 2009. http://proquest.umi.com/pqdweb?did=1885544371&sid=6&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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20

Rudd, M. Katharine. "Organization, evolution and function of alpha satellite DNA at human centromeres." Connect to text online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1091493781.

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21

Mathrubutham, Umamaheshwar. "An investigation into the use of yeast artificial chromosomes for the identification of origins of replication in human DNA." Thesis, University of Southampton, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296259.

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22

Miyagi, Mikiko. "Exploitation of bacterial artificial chromosome (BAC) libraries to enhance the efficiency of genome mapping." Thesis, Queensland University of Technology, 2002. https://eprints.qut.edu.au/37140/6/37140_Digitised%20Thesis.pdf.

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23

Buchanan, Christina Diane. "Identification and characterization of a checkpoint triggered by delayed replication in S. cerevisiae /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/10253.

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24

Farrell, Patrick James. "The development of stably transformed lepidopteran insect cell technology for both the expression of recombinant proteins and the generation of baculovirus artificial chromosomes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ34672.pdf.

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25

Guo, Yufang. "Quantitative genetic analysis for flowering time in primitive Upland cotton, Gossypium hirsutum L., and chromosome assignment of BAC-derived SSR markers." Diss., Mississippi State : Mississippi State University, 2007. http://library.msstate.edu/etd/show.asp?etd=etd-11062007-142438.

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26

Khoja, Suhail. "HSV-1 amplicon system for human artificial chromosome formation in human ES/iPS cells and pluripotency induction." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:6b04170b-f2d9-4114-9511-05a1a98ccfec.

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Development of safe and efficient approaches for gene delivery in human embryonic stem cells (hESc) and particularly in human induced pluripotent stem (hiPS) cells, which can be derived in a person-specific manner, is considered to be imperative for harnessing their full potential in both the basic and applied research. The aim of this study was to evaluate the potential of human artificial chromosome (HAC) for gene delivery and expression in hESc and hiPS cells. HAC offers many potential advantages including the provision for carrying large genes with corresponding regulatory elements to obta

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27

Wood, Lynn. "High resolution physical mapping of an 840KB region at the 5' end of the dystrophin gene by construction of ordered phage lambda sublibraries from yeast artificial chromosomes." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242219.

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28

Siva, Subramaniam Nitthiya. "An analysis of the Class 1 Gene region in sheep major histocompatibility complex." Thesis, Curtin University, 2012. http://hdl.handle.net/20.500.11937/773.

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The major histocompatibility complex (MHC) is a chromosomal region associated with immune responsiveness in vertebrates. Over four decades many studies have demonstrated important associations between MHC loci and disease resistance or susceptibility in a variety of mammals, especially humans and mice. However, characterisation of the sheep MHC has not been widely studied compared to other domestic species. Since sheep provide food and fibre to many of the world’s populations, and is a major industry in Australia a better understanding of the sheep MHC will deliver many benefits when used in c

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29

Hughes, Amanda L. "Dissecting cis and trans Determinants of Nucleosome Positioning: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/743.

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Eukaryotic DNA is packaged in chromatin, whose repeating subunit, the nucleosome, consists of an octamer of histone proteins wrapped by about 147bp of DNA. This packaging affects the accessibility of DNA and hence any process that occurs on DNA, such as replication, repair, and transcription. An early observation from genome-wide nucleosome mapping in yeast was that genes had a surprisingly characteristic structure, which has motivated studies to understand what determines this architecture. Both sequence and trans acting factors are known to influence chromatin packaging, but the relative con

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30

Hughes, Amanda L. "Dissecting cis and trans Determinants of Nucleosome Positioning: A Dissertation." eScholarship@UMMS, 2011. http://escholarship.umassmed.edu/gsbs_diss/743.

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Eukaryotic DNA is packaged in chromatin, whose repeating subunit, the nucleosome, consists of an octamer of histone proteins wrapped by about 147bp of DNA. This packaging affects the accessibility of DNA and hence any process that occurs on DNA, such as replication, repair, and transcription. An early observation from genome-wide nucleosome mapping in yeast was that genes had a surprisingly characteristic structure, which has motivated studies to understand what determines this architecture. Both sequence and trans acting factors are known to influence chromatin packaging, but the relative con

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31

ROTOMONDO, FRANCOISE. "Caracterisation de la region pericentromerique du chromosome 19 murin, a l'aide de chromosomes artificiels de levures (yacs)." Nice, 1996. http://www.theses.fr/1996NICE5012.

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Avec une longueur estimee genetiquement aux alentours de 55cm, le chromosome 19 est le plus petit des automes murins. La region pericentromerique, qui s'etend environ sur 15 cm, contient de nombreux genes impliques dans diverses pathologies murines. De plus, cette region est homologue a la bande q13 du chr11 humain, elle-meme siege de nombreuses alterations responsables de pathologies humaines variees. Nous avons entrepris, en etroite collaboration avec une autre equipe menant une etude cartographique approfondie sur 11q13, l'etablissement d'une carte physique hautement resolutive de la region

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32

Trapp, Sascha. "Klonierung und Mutagenese des Bovinen Herpesvirus Typ 1 als ein infektiöses künstliches bakterielles Chromosom (bacterial artificial chromosome, BAC)." Diss., lmu, 2003. http://nbn-resolving.de/urn:nbn:de:bvb:19-11529.

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33

Willars, Kate. "Development of targeted bacterial artificial chromosome cloning techniques." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620355.

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34

Taylor, Stephen S. "Manipulation of YACs to construct a mammalian artificial chromosome." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260750.

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35

Kotzamanis, George. "Construction of mammalian artificial chromosome vectors for expression of therapeutic genes." Thesis, Imperial College London, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411348.

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36

Young, Dorothy J. D. "S. pombe artificial chromosome (SPARC) vectors for cloning large DNA fragments." Thesis, University of Edinburgh, 1997. http://hdl.handle.net/1842/11657.

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The fusion yeast, <I>Schizosaccharomyces pombe, </I>has several potential advantages as a host for a cloning system for large DNA molecules when compared to the budding yeast, <I>Saccharomyces cerevisiae. </I>The possibility of constructing a large DNA cloning system in <I>S. pombe </I>has been investigated. <I>S. pombe </I>has 3 large chromosomes (greater than 3.5 Mb) and may therefore have the ability to carry megabase sized artificial chromosomes. In addition artificial chromosomes of less than 3.5 Mb will migrate below the host chromosomes on pulsed field gels and thus will be easier to se

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37

Fairhead, Cécile. "Proprietes et organisation des chromosomes naturels et artificiels de levure." Paris 6, 1993. http://www.theses.fr/1993PA066089.

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La levure est un eucaryote unicellulaire dont le genome contient toute l'information necessaire a la vie d'une cellule. Ce genome de petite taille est actuellement en cours de sequencage par un ensemble d'equipes internationales. Nous avons participe au sequencage du chromosome iii. L'analyse de la sequence totale du iii confirme que la levure possede un genome compact, sans sequence repetee. Cent quatre-vingt-deux phases ouvertes de lecture de plus de cent codons ont ete revelees sur ce chromosome. La plupart d'entre elles correspondent a des fonctions inconnues jusqu'alors. La grande majorit

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38

Palma, Federica Di. "Analysis and mapping of bovine MHC class I gene." Thesis, University of Reading, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248145.

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39

Hassock, Sheila Ruth. "Physical and transcriptional mapping in the distal Xq28 region of the human X chromosome." Thesis, King's College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312021.

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40

Chew, Joyce Siew Khim. "Isolation and characterization of Oreochromis niloticus DNA yeast artifical chromosome clones." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ36348.pdf.

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41

Allouis, Sebastien. "Construction of a bacterial artificial chromosome library and gene targeting in pearl millet." Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327450.

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42

Cirmirakis, A. K. "An investigation of the artificial chromosome expression system for production of recombinant proteins." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1454072/.

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The requirement to produce complex, therapeutic proteins that possess human-like post-translational modifications for treatment of disease has accelerated the use of mammalian cell lines, in particular Chinese hamster ovary (CHO) cells, in the biopharmaceutical industry. However, producing stable, high expressing cell lines quickly, reproducibly and with relative simplicity whilst achieving a desired product quality profile proves challenging. Conventional methods involve random integration of the expression vector carrying the gene of interest (GOI) into the host genome, often resulting in cl

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43

Lindqvist, Emil Gedda &amp Kalle. "Genetically modelled Artificial Neural Networks for Optical Character Recognition : An evaluation of chromosome encodings." Thesis, Blekinge Tekniska Högskola, Sektionen för datavetenskap och kommunikation, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-4504.

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Context. Custom solutions to optical character recognition problems are able to reach higher recognition rates then a generic solution by their ability to exploiting the limitations in the problem domain. Such solutions can be generated with genetic algorithms. This thesis evaluates two different chromosome encodings on an optical character recognition problem with a limited problem domain. Objectives. The main objective for this study is to compare two different chromosome encodings used in a genetic algorithm generating neural networks for an optical character recognition problem to evaluate

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44

Gill, Clare Alexandra. "Use of an ovine bacterial artificial chromosome library for the study of Bovidae genomes." Title page, table of contents and abstract only, 1999. http://web4.library.adelaide.edu.au/theses/09ANP/09anpg475.pdf.

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45

Wussow, Felix [Verfasser]. "Seamless manipulation of the varicella-zoster virus genome via bacterial artificial chromosome engineering / Felix Wussow." Kiel : Universitätsbibliothek Kiel, 2010. http://d-nb.info/1019951206/34.

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46

Tanaka, Karo. "Characterisation of the murine homologue of the CIC-5 gene : a voltage-gated chloride channel implicated in human X-linked hereditary nephrolithiasis." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302335.

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47

Martins, Nuno Miguel Marques Vitória Cabrita. "HACking centrochromatin : on the relationship between centromeres and repressive chromatin." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/17599.

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The centromere is a chromosomal locus required for accurate segregation of sister chromatids during cell division. They are maintained epigenetically in most eukaryotes, by incorporating the H3 variant CENP-A, and can, in rare instances, change location on the chromosome throughout generations. Centromeres are transcribed, and an active transcription chromatin signature is required for centromere maintenance. For this reason, insight into the nature of this so-called “centrochromatin” is essential for understanding a centromere’s place in the chromosome. The body of work contained in this thes

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48

Cunha, Kelly de Paula. "Aplicação de mapas auto-organizáveis na classificação de aberrações cromossômicas utilizando imagens de cromossomos humanos submetidos à radiação ionizante." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/85/85133/tde-05062015-140631/.

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O presente trabalho é resultado da colaboração de pesquisadores do Centro de Engenharia Nuclear (CEN) e de pesquisadores do Centro de Biotecnologia (CB), ambos pertencentes ao IPEN, para o desenvolvimento de uma metodologia que visa auxiliar os profissionais citogeneticistas fornecendo uma ferramenta que automatize parte da rotina necessária para a avaliação qualitativa e quantitativa de danos biológicos em termos de aberração cromossômica. A técnica citogenética, sobre a qual esta ferramenta é desenvolvida, é a técnica de aberrações cromossômicas. Nela, são realizadas preparações citológicas

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49

Christensen, Heather R. "Molecular and Integrated Systems Physiology of Prolactin." University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1314040076.

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50

McCullough, Kevin. "EXPLORING THE RELATIONSHIP OF THE CLOSENESS OF A GENETIC ALGORITHM’S CHROMOSOME ENCODING TO ITS PROBLEM SPACE." DigitalCommons@CalPoly, 2010. https://digitalcommons.calpoly.edu/theses/247.

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For historical reasons, implementers of genetic algorithms often use a haploid binary primitive type for chromosome encoding. I will demonstrate that one can reduce development effort and achieve higher fitness by designing a genetic algorithm with an encoding scheme that closely matches the problem space. I will show that implicit parallelism does not result in binary encoded chromosomes obtaining higher fitness scores than other encodings. I will also show that Hamming distances should be understood as part of the relationship between the closeness of an encoding to the problem instead of

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